@article{2-s2.0-85152003184,

title = {Differences in hemicellulose composition and pectin detection in Eocene and Miocene xylites},

author = { L. Marynowski and M. Goryl and M. Lempart-Drozd and M. Bucha and M.A. Majewski and M. Stępień and R. Loręc and J.J. Brocks and B.R.T. Simoneit},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85152003184&doi=10.1016%2fj.chemgeo.2023.121416&partnerID=40&md5=8c204141aef9c55417784ccfe863983d},

doi = {10.1016/j.chemgeo.2023.121416},

issn = {00092541},

year  = {2023},

date = {2023-01-01},

journal = {Chemical Geology},

volume = {624},

publisher = {Elsevier B.V.},

abstract = {The composition of hemicelluloses differ considerably in extant hardwood and softwood. In this study, we demonstrate that there are also significant differences between the hemicellulose composition of angiosperm and gymnosperm fossil wood (Eocene to Miocene). Because of the excellent preservation and high hemicellulose contents, Fourier-transform infrared (FT-IR) spectroscopy of xylites shows bands that are characteristic of various hemicelluloses and pectin (despite the overlapping of cellulose and lignin IR bands). In particular, fossil softwood samples show IR bands typical of mannose-containing hemicellulose. The composition of hemicellulose is determined through methanolysis gas chromatography–mass spectrometry (GC–MS). The results correspond with those of extant softwood and hardwood hemicelluloses because of the excellent preservation of 13–35 Myr fossil wood deposited under reducing marine conditions. The main saccharide building blocks found in angiosperm Eocene and Miocene wood are glucose and xylose. In contrast, mannose, galactose, and glucose are dominant in gymnosperms. The relationship among xylose, mannose, and rhamnose suitably differentiates both fossil and extant woods. Moreover, the relatively high contents of rhamnose and galacturonic acid identified mainly in angiosperms indicate the presence of preserved pectin, which has not been previously documented in fossil organic matter. Nuclear magnetic resonance (NMR) spectroscopy reveals the presence of xylogalacturonan fragments in a separate fraction of the angiosperm Eocene wood. The presence of pectin, a significantly labile carbohydrate polymer, confirms the perfect preservation of fossil wood in marine deposits. This study sheds new light on the identification of xylite affinities based on their hemicellulose composition and promotes research toward the palaeochemotaxonomy of fossil plants. © 2023},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85107339961,

title = {Evaluation of acidogenesis products’ effect on biogas production performed with metagenomics and isotopic approaches},

author = { A. Detman and M. Bucha and L. Treu and A. Chojnacka and Ł. Pleśniak and A. Salamon and E.B. Łupikasza and R. Gromadka and J. Gawor and A. Gromadka and W. Drzewicki and M. Jakubiak and M. Janiga and I. Matyasik and M.K. Błaszczyk and M.O. Jędrysek and S. Campanaro and A. Sikora},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107339961&doi=10.1186%2fs13068-021-01968-0&partnerID=40&md5=874a262863a4c7bc7faf512bf08736a6},

doi = {10.1186/s13068-021-01968-0},

issn = {17546834},

year  = {2021},

date = {2021-01-01},

journal = {Biotechnology for Biofuels},

volume = {14},

number = {1},

publisher = {BioMed Central Ltd},

abstract = {Background: During the acetogenic step of anaerobic digestion, the products of acidogenesis are oxidized to substrates for methanogenesis: hydrogen, carbon dioxide and acetate. Acetogenesis and methanogenesis are highly interconnected processes due to the syntrophic associations between acetogenic bacteria and hydrogenotrophic methanogens, allowing the whole process to become thermodynamically favorable. The aim of this study is to determine the influence of the dominant acidic products on the metabolic pathways of methane formation and to find a core microbiome and substrate-specific species in a mixed biogas-producing system. Results: Four methane-producing microbial communities were fed with artificial media having one dominant component, respectively, lactate, butyrate, propionate and acetate, for 896 days in 3.5-L Up-flow Anaerobic Sludge Blanket (UASB) bioreactors. All the microbial communities showed moderately different methane production and utilization of the substrates. Analyses of stable carbon isotope composition of the fermentation gas and the substrates showed differences in average values of δ13C(CH4) and δ13C(CO2) revealing that acetate and lactate strongly favored the acetotrophic pathway, while butyrate and propionate favored the hydrogenotrophic pathway of methane formation. Genome-centric metagenomic analysis recovered 234 Metagenome Assembled Genomes (MAGs), including 31 archaeal and 203 bacterial species, mostly unknown and uncultivable. MAGs accounted for 54%–67% of the entire microbial community (depending on the bioreactor) and evidenced that the microbiome is extremely complex in terms of the number of species. The core microbiome was composed of Methanothrix soehngenii (the most abundant), Methanoculleus sp., unknown Bacteroidales and Spirochaetaceae. Relative abundance analysis of all the samples revealed microbes having substrate preferences. Substrate-specific species were mostly unknown and not predominant in the microbial communities. Conclusions: In this experimental system, the dominant fermentation products subjected to methanogenesis moderately modified the final effect of bioreactor performance. At the molecular level, a different contribution of acetotrophic and hydrogenotrophic pathways for methane production, a very high level of new species recovered, and a moderate variability in microbial composition depending on substrate availability were evidenced. Propionate was not a factor ceasing methane production. All these findings are relevant because lactate, acetate, propionate and butyrate are the universal products of acidogenesis, regardless of feedstock. © 2021, The Author(s).},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85111334635,

title = {Preservation of hemicellulose remnants in sedimentary organic matter},

author = { L. Marynowski and M. Bucha and M. Lempart-Drozd and M. Stępień and M. Kondratowicz and J. Smolarek-Lach and M. Rybicki and M. Goryl and J.J. Brocks and B.R.T. Simoneit},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111334635&doi=10.1016%2fj.gca.2021.07.003&partnerID=40&md5=3db5f77e50b98e7da685875bb2ec57e8},

doi = {10.1016/j.gca.2021.07.003},

issn = {00167037},

year  = {2021},

date = {2021-01-01},

journal = {Geochimica et Cosmochimica Acta},

volume = {310},

pages = {32-46},

publisher = {Elsevier Ltd},

abstract = {Cellulose has been identified in fossil material, but the preservation potential of hemicelluloses, which are less resistant to decomposition than cellulose, is generally considered very low. Here we present the discovery of hemicellulose in Miocene xylites (fossil wood) and Cretaceous xylo-detritic coals from Poland. The main building blocks of hemicelluloses in softwood are mannose, glucose, xylose with lesser amounts of galactose and arabinose. These saccharides were detected in the coals and xylites using independent geochemical methods. Based on chemical analysis, the lignites contained significant holocellulose (22–37%), α-cellulose (8–29%) and hemicellulose (7–13%). In the smoke from a xylite burn test, levoglucosan and mannosan were dominant, the latter a specific hemicellulose alteration product. Glucose and mannose products dominated after methanolysis, with minor galactose and xylose. The main hemicellulosic polysaccharides in lignite appear to be glucomannan and/or galactoglucomannan but with a lower mannose content, possibly connected to wood degradation by fungi. The preservation of hemicelluloses in fossil material may be due to structural interconnection between lignin, cellulose and hemicellulose (i.e. lignocellulose), common in extant wood. This is the first documentation of hemicelluloses in fossil material. Our results show that not only cellulose, but also hemicelluloses can persist for millions of years under favorable conditions with only minor structural changes due to slow microbial and/or diagenetic decay. In fossil wood, types of hemicellulose can help assess whether the ancient plants were related to gymnosperms or angiosperms. © 2021 Elsevier Ltd},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85102972550,

title = {Monoterpenylabietenoids, novel biomarkers from extant and fossil Taxodioideae and sedimentary rocks},

author = { B.R.T. Simoneit and M. Rybicki and M. Goryl and M. Bucha and A. Otto and L. Marynowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102972550&doi=10.1016%2fj.orggeochem.2020.104172&partnerID=40&md5=f330e429889bbdaac445f551219bde7e},

doi = {10.1016/j.orggeochem.2020.104172},

issn = {01466380},

year  = {2021},

date = {2021-01-01},

journal = {Organic Geochemistry},

volume = {154},

publisher = {Elsevier Ltd},

abstract = {The presence of 7-p-cymenylferruginol and its diagenetic biomarkers occurring in Cenozoic fossil plants and Mesozoic siltstones and coals has been studied using gas chromatography-mass spectrometry. Both 7α- and 7β-p-cymenylferruginol isomers were the dominant natural products, with two novel minor additional isomers, namely 3α- and 3β-p-cymenylferruginol, and two hydrocarbons, 7α- and 7β-p-cymenyldehydroabietane. The other tentatively assigned aromatic hydrocarbons were 9,10-p-cymenylretene and 9-p-cymenylsimonellite, which were present in Upper Cretaceous and Lower Jurassic sedimentary rocks with their further dehydrogenation products. Diterpenoid dimers were also tentatively identified in trace amounts, with 11- and 14-ferruginylferruginol as the dominant compounds. Our results show that the p-cymenylferruginols and p-cymenyldehydroabietanes are not thermally stable and that their occurrence in sedimentary rocks is controlled by the maturity of the organic matter (OM). Also, chamaecydin, identified for the first time in pre-Paleogene strata, was not detectable when the OM maturity based on vitrinite reflectance was higher than ∼0.45–0.5% Rr. © 2020 Elsevier Ltd},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85088918438,

title = {Microbial methane formation from different lithotypes of Miocene lignites from the Konin Basin, Poland: Geochemistry of the gases and composition of the microbial communities},

author = { M. Bucha and A. Detman and Ł. Pleśniak and W. Drzewicki and D. Kufka and A. Chojnacka and D. Mielecki and J. Krajniak and M.O. Jędrysek and A. Sikora and L. Marynowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088918438&doi=10.1016%2fj.coal.2020.103558&partnerID=40&md5=442f5f2d3c0f2c443cd6208b7f56e7e9},

doi = {10.1016/j.coal.2020.103558},

issn = {01665162},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Coal Geology},

volume = {229},

publisher = {Elsevier B.V.},

abstract = {The present research compares the yield of microbial methane production from different lithotypes of Miocene lignite from the Konin Basin in Poland and attempts to establish an understanding of the processes responsible for methanogenesis. A series of batch experiments were carried out with detritic and xylitic lignites inoculated with microorganisms from an external source (the anaerobic chamber of a wastewater treatment plant in a sugar factory). Biogas volume, concentration and stable carbon isotopes of microbial methane were measured. It was found that detritic lignites are a slightly more suitable raw material for microbial methane production than xylites. Methane yield for detritic lignites equalled 14.3 μmol CH4/g of total organic carbon (TOC); for xylites, 13.7 μmol CH4/g of TOC. The mean δ13C(CH4) value in experiments with detritic coal from Konin equalled −36.3‰; for fossil wood fragments, −47.3‰ and −42.7‰. We suppose that differences in mean δ13C(CH4) values from the biodegradation of different lithotypes of lignite from the Konin Basin most probably depend on cellulose and lignin content (%). The holocellulose content in xylites decreased over time, suggesting the important role of the enzymatic hydrolysis of cellulose to glucose. Methane from detritic lignite was formed due to lignin decomposition. Methane-producing microbial communities were dominated by Bacteria mainly from the phyla Proteobacteria (Alpha-; Beta- and Gamma- or Deltaproteobacteria), Firmicutes (Clostridia and Bacilli), Actinobacteria, and Bacteroidetes. Archaea constituted only 2–6% of the microbial community, including Methanosarcinales, Methanomicrobiales, and Methanobacteriales. The data presented here show no clear correlations between lignite type and specific bacterial or archaeal taxa. However, certain tendencies were observed. Exiguobacterium and Pseudomonas are more abundant in xylites, Rhizobacteriaceae in detritic lignites. This indicates the complexity and diversity of lignite material and processes leading to lignite degradation. In the presented research model, anaerobic oxidation of methane may occur. © 2020 Elsevier B.V.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85081047423,

title = {Terpenoid biomarkers of ambers from Miocene tropical paleoenvironments in Borneo and of their potential extant plant sources},

author = { B.R.T. Simoneit and D.R. Oros and Ł. Karwowski and Ł. Szendera and J. Smolarek-Lach and M. Goryl and M. Bucha and M. Rybicki and L. Marynowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081047423&doi=10.1016%2fj.coal.2020.103430&partnerID=40&md5=bbc4d9da365e778a7079a51113ac8268},

doi = {10.1016/j.coal.2020.103430},

issn = {01665162},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Coal Geology},

volume = {221},

publisher = {Elsevier B.V.},

abstract = {The chemical composition of ambers is highly diverse, characterized by the occurrence of a variety of terpenoids including: mono-, sesqui-, di-, and triterpenoids. The direct analyses of their chemical constituents in total extracts using polar solvents permits the elucidation of unaltered natural products, which are characteristic of the source plants or paleobiome. At this time, knowledge is limited about the plant origins of fossil resins that formed in tropical climatic conditions. Here, we present the complex chemical characteristics of Miocene fossil resins (resinites; termed here as ambers) from the tropics of Kalimantan (Borneo; Indonesia). Extant plant resins from the same geoclimatic region were also analyzed to identify the potential botanical sources of the ambers. Gas chromatography-mass spectrometry (GC–MS) analyses of total extracts (silylated and methylated) of natural and amber samples were carried out and compared with standard compounds. The main producers of resins forming these Miocene ambers were angiosperms - probably resins of Shorea and less likely Hopea, but not Dipterocarpus species. The key chemotaxonomic marker, present in the ambers and extant Shorea species, was asiatic acid. All samples were composed of sesquiterpenoids and triterpenoids in various proportions, without diterpenoids, characteristic for flowering plants. The sesquiterpenoids in the resins of both ambers and extant plants were primarily natural products with the cadinane skeleton. The triterpenoids of the extant resins of the Dipterocarpaceae and Miocene ambers were characterized by a prevalence of ursane over oleanane types. Polymerization of cadinoids in resins from Shorea species and in the ambers was not extensive. Based on the amber compositions we conclude that the molecular alteration of the Miocene deposits from Kalimantan is rather low, but differs depending on their location. © 2020 Elsevier B.V.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85057341552,

title = {Trehalose, mannitol and arabitol as indicators of fungal metabolism in Late Cretaceous and Miocene deposits},

author = { L. Marynowski and M. Goryl and M. Bucha and J. Smolarek-Lach and A. Detman and A. Sikora and A. Chojnacka and B.R.T. Simoneit},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057341552&doi=10.1016%2fj.coal.2018.11.003&partnerID=40&md5=9b63c4825a8532e9e734e81fc419aff9},

doi = {10.1016/j.coal.2018.11.003},

issn = {01665162},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Coal Geology},

volume = {201},

pages = {51-61},

publisher = {Elsevier B.V.},

abstract = {Trehalose, mannitol and arabitol are the main saccharides of extant fungal metabolism, but their occurrence and distribution in geological materials have rarely been considered. Here, we identify these sugars in Miocene lignites and for the first time in Late Cretaceous mudstones and coals. The co-occurrence of trehalose, mannitol and arabitol in the sedimentary rocks investigated suggests their fungal origin, because these three saccharides are major compounds present in most modern fungi, including the very common mycorrhizal and wood-rotting groups. Therefore, we conclude that these sugars should be treated as new fungal biomarkers (biomolecules) present in geological rocks. Trehalose and mannitol are major compounds in total extracts of the samples and a sum of their concentration reaches 4.6 μg/g of sample. The arabitol concentrations do not exceed 0.5 μg/g, but in contrast to trehalose, the concentration correlates well with mannitol (R2 = 0.94), suggesting that they have the same, translocatory role in fungi. Based on the trehalose vs. mannitol and arabitol distributions in Cretaceous samples and their comparison with data for modern fungi, we preliminarily conclude that the coal seams from the Rakowice Małe (SW Poland) section were formed during warmer climatic periods than the overlying sediments. Furthermore, no DNA could be isolated from the samples of lignites and overlying sediments, whereas it was abundant in the control samples of maple, birch and oak wood degraded by fungi. This indicates an absence of recent fungi responsible for decay in lignites and implies that the saccharide origin is connected with ancient fungi. Other sugar alcohols and acids like D-pinitol, quinic acid and shikimic acid, were found for the first time in sedimentary rocks, and their source is inferred to be from higher plants, most likely conifers. The preservation of mono- and disaccharides of fungal origins in pre-Palaeogene strata implies that compounds previously thought as unstable can survive for tens to hundreds of millions of years without structural changes in immature rocks unaffected by secondary processes. © 2018},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85055562699,

title = {Decomposition of carbon-bearing compounds and their influence on methane formation in a lignite incubation experiment},

author = { M. Bucha and D. Kufka and Ł. Pleśniak and J. Krajniak and K. Kubiak and L. Marynowski and M.K. Błaszczyk and M.O. Jędrysek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055562699&doi=10.1080%2f01490451.2018.1506528&partnerID=40&md5=e0bd2a2a6daae2b3596e1aedb965a22c},

doi = {10.1080/01490451.2018.1506528},

issn = {01490451},

year  = {2019},

date = {2019-01-01},

journal = {Geomicrobiology Journal},

volume = {36},

number = {1},

pages = {63-74},

publisher = {Taylor and Francis Inc.},

abstract = {Carbon-bearing compounds (glucose; sodium acetate; methanol; yeast extract; and nutrient broth) were added in different proportions to cultures to stimulate methanogenesis in a lignite incubation experiment. Their addition significantly influenced the isotopic composition of methane generated during the fermentation of lignite. Glucose was degraded mainly in the first 2 weeks of incubation, when the atmospheric air was present in the headspace and used for biomass growth. Sodium acetate, methanol, and, presumably, lignite were decomposed in the next phase, in which anaerobic conditions occurred. The simultaneous decomposition of sodium acetate and methanol (as single substrates or as a mixture) with lignite resulted in the formation of methane with δ 13 C(CH 4 ) values typical for methyl-type fermentation. The identification of decomposed compounds in the mixture of sodium acetate and methanol was accomplished via isotopic analysis of carbon and hydrogen in the methane. The δ 2 H(CH 4 ) values in the case of methanol biodegradation were characterized by a negative trend over time, in contrast to a positive trend observed when sodium acetate decomposed. This observation may help to identify a very good tracer for the determination of methane precursors during methyl-type fermentation. © 2018, © 2018 Taylor & Francis Group, LLC.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85073757866,

title = {Stable isotopes of C and H in methane fermentation of agriculture substrates at different temperature conditions},

author = { D. Kufka and M. Bucha and Ł. Pleśniak and M.O. Jędrysek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073757866&doi=10.1515%2fgeo-2019-0039&partnerID=40&md5=a2261dd0f0493070345678ed4ef5508d},

doi = {10.1515/geo-2019-0039},

issn = {23915447},

year  = {2019},

date = {2019-01-01},

journal = {Open Geosciences},

volume = {11},

number = {1},

pages = {471-481},

publisher = {De Gruyter Open Ltd},

abstract = {Agricultural substrates (maize silage and cattle manure) were used to carry out methane fermentation process in bioreactors under laboratory conditions. Identical mixtures of these substrates were incubated for 43 days at 20, 30 and 40°C to determine how different temperature conditions affect the δ13C(CH4), δH(CH4), and δ13C(CO2) values. To ensure correct anaerobic digestion, the following parameters of the organic substrates and fermentation solutions were monitored: total organic carbon (TOC), volatile solids (VS), volatile fatty acids (VFA), chemical oxygen demand (COD) and carbon to nitrogen ratio (C/N). The variants with higher incubation temperature yielded higher amounts of biogas (20°C=84.5; 30°C=101.8 and 40°C=133.3 dm3/kg VS). In the case of gas products of methane fermentation, it was observed that the higher temperature of incubation affects the depletion in heavy isotopes. At 20°C, 30°C, and 40°C mean values of δ13C(CH4) reached -26.4, -29.7, and -35.4‰, respectively. Mean values of δ2H(CH4) were -311.6, -354.0, and -398.5permil, and of δ13C(CO2) +8.9, +3.7, and -2.3‰, respectively. Moreover, the apparent fractionation coefficient α13C(CO2-CH4) were calculated, which decreased when the temperature increased. This isotopic tool was used to identify acetoclastic reaction as a dominant methanogenesis pathway. Observed changes in the isotopic composition of gaseous products obtained at different incubation temperatures may indicate decomposition of different carbon sources (e.g. lactate; propionate) to acetate and its fermentation by acetoclastic methanogens. It is possible that this was also related to the observation of the various metabolic models due to the varied methanogenic community composition. © 2019 Dominika Kufka et al.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85051109042,

title = {Lignite biodegradation under conditions of acidic molasses fermentation},

author = { A. Detman and M. Bucha and B.R.T. Simoneit and D. Mielecki and C. Piwowarczyk and A. Chojnacka and M.K. Błaszczyk and M.O. Jędrysek and L. Marynowski and A. Sikora},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051109042&doi=10.1016%2fj.coal.2018.07.015&partnerID=40&md5=1f8699de70dced6cab2f6d1692fad27e},

doi = {10.1016/j.coal.2018.07.015},

issn = {01665162},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Coal Geology},

volume = {196},

pages = {274-287},

publisher = {Elsevier B.V.},

abstract = {Lignite is difficult to degrade, thus stimulation of the autochthonous lignite microflora and introduction of additional microorganisms are required for lignite decomposition. Here, a packed bed reactor, filled with lignite samples from the Konin region (central Poland) was supplied continuously with M9 medium, supplemented with molasses (a by-product from the sugar industry), for 124 days to stimulate the autochthonous lignite microflora. Acidic fermentation of molasses was observed in the bioreactor. The simultaneous decomposition of lignite occurred under this acidic molasses fermentation condition. Our results show decay of free (non-bound) organic compounds during anaerobic lignite biodegradation. The concentrations of n-alkanes, n-alkanols, n-alkanoic acids, diterpenoids, triterpenoids and steroids present in non-biodegraded samples decreased significantly (some compounds to zero) during biodegradation. Interestingly, other compound classes like phenols, ketones and certain organic compounds increased. We interpret this phenomenon as a gradual decomposition of polymers, lignin and cellulose, present in the lignite. These changes resulted from microbial activity since they were not observed in pure solutions of short-chain fatty acids. The 16SrRNA profiling of the microbial community selected in the bioreactor revealed that the dominant bacteria belonged to the Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes, furthermore representatives of 16 other phyla were also found. All the known taxa of lignocellulolytic bacteria were represented in the microbial community. Synergistic relations between bacteria fermenting molasses and bacteria degrading lignite are assumed. The results confirm lignin degradation in acidic medium by bacteria under anaerobic conditions. © 2018 Elsevier B.V.},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85045929865,

title = {Methane-yielding microbial communities processing lactate-rich substrates: A piece of the anaerobic digestion puzzle},

author = { A. Detman and D. Mielecki and Ł. Pleśniak and M. Bucha and M. Janiga and I. Matyasik and A. Chojnacka and M.O. Jędrysek and M.K. Błaszczyk and A. Sikora},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045929865&doi=10.1186%2fs13068-018-1106-z&partnerID=40&md5=4fe24fc45a38e860e246e47713295918},

doi = {10.1186/s13068-018-1106-z},

issn = {17546834},

year  = {2018},

date = {2018-01-01},

journal = {Biotechnology for Biofuels},

volume = {11},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: Anaerobic digestion, whose final products are methane and carbon dioxide, ensures energy flow and circulation of matter in ecosystems. This naturally occurring process is used for the production of renewable energy from biomass. Lactate, a common product of acidic fermentation, is a key intermediate in anaerobic digestion of biomass in the environment and biogas plants. Effective utilization of lactate has been observed in many experimental approaches used to study anaerobic digestion. Interestingly, anaerobic lactate oxidation and lactate oxidizers as a physiological group in methane-yielding microbial communities have not received enough attention in the context of the acetogenic step of anaerobic digestion. This study focuses on metabolic transformation of lactate during the acetogenic and methanogenic steps of anaerobic digestion in methane-yielding bioreactors. Results: Methane-yielding microbial communities instead of pure cultures of acetate producers were used to process artificial lactate-rich media to methane and carbon dioxide in up-flow anaerobic sludge blanket reactors. The media imitated the mixture of acidic products found in anaerobic environments/digesters where lactate fermentation dominates in acidogenesis. Effective utilization of lactate and biogas production was observed. 16S rRNA profiling was used to examine the selected methane-yielding communities. Among Archaea present in the bioreactors, the order Methanosarcinales predominated. The acetoclastic pathway of methane formation was further confirmed by analysis of the stable carbon isotope composition of methane and carbon dioxide. The domain Bacteria was represented by Bacteroidetes, Firmicutes, Proteobacteria, Synergistetes, Actinobacteria, Spirochaetes, Tenericutes, Caldithrix, Verrucomicrobia, Thermotogae, Chloroflexi, Nitrospirae, and Cyanobacteria. Available genome sequences of species and/or genera identified in the microbial communities were searched for genes encoding the lactate-oxidizing metabolic machinery homologous to those of Acetobacterium woodii and Desulfovibrio vulgaris. Furthermore, genes for enzymes of the reductive acetyl-CoA pathway were present in the microbial communities. Conclusions: The results indicate that lactate is oxidized mainly to acetate during the acetogenic step of AD and this comprises the acetotrophic pathway of methanogenesis. The genes for lactate utilization under anaerobic conditions are widespread in the domain Bacteria. Lactate oxidation to the substrates for methanogens is the most energetically attractive process in comparison to butyrate, propionate, or ethanol oxidation. © 2018 The Author(s).},

note = {47},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040625332,

title = {Methanogenic fermentation of lignite with carbon-bearing additives, inferred from stable carbon and hydrogen isotopes},

author = { M. Bucha and M.O. Jędrysek and D. Kufka and Ł. Pleśniak and L. Marynowski and K. Kubiak and M.K. Błaszczyk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040625332&doi=10.1016%2fj.coal.2017.11.020&partnerID=40&md5=6f1e34f6e03a0b600146325b3a2b5209},

doi = {10.1016/j.coal.2017.11.020},

issn = {01665162},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Coal Geology},

volume = {186},

pages = {65-79},

publisher = {Elsevier B.V.},

abstract = {Lignite from the Konin area (Poland) was used as a substrate for incubation experiments to evaluate its potential for simulation of biogenic methane production. Lignite was incubated with a bacterial inoculum enriched from lake sediments, mineral media, and various supplemental components for microbial life. Additives, such as acetate, methanol, glucose, nutrient broth, and yeast extract, can significantly increase methane production. At the same time, biodegradation of these additional carbon sources leads to overestimation of methane yield. In this paper, selected geochemical properties (total organic carbon content; stable isotopic composition of carbon δ13C) were analyzed in order to evaluate changes in the organic matter of fermented lignite. Stable isotope analysis of carbon and hydrogen was applied in order to identify sources of methane and carbon dioxide formation. TOC decreased in range from 1.4 to 9.6% in lignite after fermentation. The δ13C value of lignite used in the experiments (−25.2‰) decreased after incubation to values in range from −27.1 to −26.2‰. Methane yield per g of TOC (lignite + organic carbon in nutrients) ranged from 0.47 to 2.60 mM/g. Glucose, acetate, and methanol significantly increased biogas production. Nutrient broth and yeast extract were not a source of organic carbon for methane formation, but their presence enhanced biogas production. Values of δ13C(CH4) and δ2H(CH4) across incubation conditions ranged from −70.2 to −24.2‰ and from −396.6 to −290.5‰ respectively. Values of δ13C(CO2) ranged from –55.2 to 45.0‰. The high level of variation of δ13C(CH4) and δ13C(CO2) suggests mixing of gases from different carbon sources during incubation, but can also be caused by mixing of metabolic modes by the microbial community. Understanding the δ2H(CH4) variation is even more difficult than the δ13C(CH4) and δ13C(CO2), since many substrates often contain exchangeable hydrogen (e.g. in water; lignite; and elements of nutrients). The combined values of δ13C(CH4) and δ13C(CO2) suggests that the dominant methanogenesis pathway in our experiments may be acetate fermentation. In biodegraded lignite, high relative concentrations of p-cresol (one of the most abundant in the sample) and n-(2-acetylphenyl)formamide were identified. These compounds are most probably lignin decomposition products, or, in the case of the latter, bacterial by-products or remnants. Organic compounds with low molecular weights, n-alkanes, and biomolecules including ferruginol, sugiol, and 6,7-dehydroferruginol, as well as amyrins and tocopherols, were preferentially degraded. The potential for methane production from lignite spiked with carbon-bearing additives is at least one magnitude lower than that from agricultural wastes. The lignite utilization as the single substrate for methanogenic fermentation is economically unprofitable. Mixing of lignite with the external substrate as biomass may be an alternative for consideration and future research. © 2017 Elsevier B.V.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85037356247,

title = {Occurrence and significance of mono-, di- and anhydrosaccharide biomolecules in Mesozoic and Cenozoic lignites and fossil wood},

author = { L. Marynowski and M. Bucha and J. Smolarek-Lach and M. Wendorff-Belon and B.R.T. Simoneit},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85037356247&doi=10.1016%2fj.orggeochem.2017.11.008&partnerID=40&md5=2d3cf3128c5a6ef57eaa5c7b988222f1},

doi = {10.1016/j.orggeochem.2017.11.008},

issn = {01466380},

year  = {2018},

date = {2018-01-01},

journal = {Organic Geochemistry},

volume = {116},

pages = {13-22},

publisher = {Elsevier Ltd},

abstract = {Mono-, di- and polysaccharides are common constituents of living organisms, but their occurrence and state of preservation in geological materials have only rarely been considered. Here, we present the monosaccharide, and for the first time the di- and anhydrosaccharide, identifications and distributions in Middle Miocene lignite and Middle Jurassic fossil wood samples. Detritic lignites contain fructose and glucose as dominant monosaccharides, and sucrose and trehalose as important disaccharides. Xylites contain monosaccharides (arabinose; arabinofuranose; glucose; and minor xylose and fructose), saccharols (erythritol; arabitol and mannitol), and also some disaccharides. The Middle Jurassic fossil wood samples contain glucose, glucofuranose and levoglucosan. The high content of holocellulose (up to 55 wt%) and co-occurrence of characteristic monosaccharides as arabinose, xylose and mannose in xylites suggests that not only cellulose, but also hemicellulose was preserved in samples as old as 13 Ma. Compounds like trehalose and mannitol appear to be products of wood-degrading fungi. Surprisingly, glucose, the most stable monosaccharide, and levoglucosan can occur in much older organic matter (ca. 168 Ma) as products from cellulose degradation, and possibly a remnant from wildfire burning of wood, respectively. Our findings confirm that saccharides can be preserved under favorable conditions in sedimentary organic matter of the Mesozoic to the Cenozoic eras, and can be used as specific biomarkers of cellulose and hemicellulose degradation, fungal metabolism, and wildfire events. However, we cannot exclude the possibility that at least part of the saccharides may be preserved in sedimentary rocks as the free compounds, common in plants and microorganisms. © 2017 Elsevier Ltd},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84940745916,

title = {A Special Issue (Part-II): Mafic-ultramafic rocks and alkaline-carbonatitic magmatism and associated hydrothermal mineralization - Dedication to Lia N. Kogarko},

author = { L.G. Gwalani and K. Rogers and F. Stoppa and P.J. Downes and K. Randive and K.R. Hari and M. Bucha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940745916&doi=10.1515%2fgeo-2015-0026&partnerID=40&md5=2b7bf0adde27958a154282feffe9ebde},

doi = {10.1515/geo-2015-0026},

issn = {23915447},

year  = {2015},

date = {2015-01-01},

journal = {Open Geosciences},

volume = {7},

number = {1},

pages = {193-196},

publisher = {De Gruyter Open Ltd},

abstract = {[No abstract available]},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}