• dr Michał Krzyżowski
Stanowisko: Adiunkt
Jednostka: Wydział Nauk Przyrodniczych
Adres: 40-032 Katowice, ul. Bankowa 9
Piętro: II
Numer pokoju: 219
Telefon: (32) 359 1278
E-mail: michal.krzyzowski@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 57191821718
Publikacje z bazy Scopus
2023
Baran, B.; Krzyżowski, M.; Rádai, Z.; Francikowski, J.; Hohol, M.
In: Animal Cognition, vol. 26, no. 3, pp. 755-770, 2023, ISSN: 14359448.
@article{2-s2.0-85141777425,
title = {Geometry-based navigation in the dark: layout symmetry facilitates spatial learning in the house cricket, Acheta domesticus, in the absence of visual cues},
author = { B. Baran and M. Krzyżowski and Z. Rádai and J. Francikowski and M. Hohol},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85141777425&doi=10.1007%2fs10071-022-01712-7&partnerID=40&md5=c8ccbf1255c8147dabdc9685104561da},
doi = {10.1007/s10071-022-01712-7},
issn = {14359448},
year = {2023},
date = {2023-01-01},
journal = {Animal Cognition},
volume = {26},
number = {3},
pages = {755-770},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {The capacity to navigate by layout geometry has been widely recognized as a robust strategy of place-finding. It has been reported in various species, although most studies were performed with vision-based paradigms. In the presented study, we aimed to investigate layout symmetry-based navigation in the house cricket, Acheta domesticus, in the absence of visual cues. For this purpose, we used a non-visual paradigm modeled on the Tennessee Williams setup. We ensured that the visual cues were indeed inaccessible to insects. In the main experiment, we tested whether crickets are capable of learning to localize the centrally positioned, inconspicuous cool spot in heated arenas of various shapes (i.e.; circular; square; triangular; and asymmetric quadrilateral). We found that the symmetry of the arena significantly facilitates crickets’ learning to find the cool spot, indicated by the increased time spent on the cool spot and the decreased latency in locating it in subsequent trials. To investigate mechanisms utilized by crickets, we analyzed their approach paths to the spot. We found that crickets used both heuristic and directed strategies of approaching the target, with the dominance of a semi-directed strategy (i.e.; a thigmotactic phase preceding direct navigation to the target). We propose that the poor performance of crickets in the asymmetrical quadrilateral arena may be explained by the difficulty of encoding its layout with cues from a single modality. © 2022, The Author(s).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The capacity to navigate by layout geometry has been widely recognized as a robust strategy of place-finding. It has been reported in various species, although most studies were performed with vision-based paradigms. In the presented study, we aimed to investigate layout symmetry-based navigation in the house cricket, Acheta domesticus, in the absence of visual cues. For this purpose, we used a non-visual paradigm modeled on the Tennessee Williams setup. We ensured that the visual cues were indeed inaccessible to insects. In the main experiment, we tested whether crickets are capable of learning to localize the centrally positioned, inconspicuous cool spot in heated arenas of various shapes (i.e.; circular; square; triangular; and asymmetric quadrilateral). We found that the symmetry of the arena significantly facilitates crickets’ learning to find the cool spot, indicated by the increased time spent on the cool spot and the decreased latency in locating it in subsequent trials. To investigate mechanisms utilized by crickets, we analyzed their approach paths to the spot. We found that crickets used both heuristic and directed strategies of approaching the target, with the dominance of a semi-directed strategy (i.e.; a thigmotactic phase preceding direct navigation to the target). We propose that the poor performance of crickets in the asymmetrical quadrilateral arena may be explained by the difficulty of encoding its layout with cues from a single modality. © 2022, The Author(s).
2022
Woźnica, A.; Karczewski, J.; Bernaś, T.; Świątek, P.; Drab, M.; Surma, R.; Krzyżowski, M.; Łozowski, B.; Gwiazda, R.; Libera, M.; Absalon, D.; Babczyńska, A.
In: European Zoological Journal, vol. 89, no. 1, pp. 1002-1017, 2022, ISSN: 24750263, (1).
@article{2-s2.0-85135441745,
title = {The spatial structure (3D) and mechanical properties of the sponge Spongilla lacustris L. (Porifera: Spongillida) skeleton as a potential tensegral architecture},
author = { A. Woźnica and J. Karczewski and T. Bernaś and P. Świątek and M. Drab and R. Surma and M. Krzyżowski and B. Łozowski and R. Gwiazda and M. Libera and D. Absalon and A. Babczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135441745&doi=10.1080%2f24750263.2022.2105964&partnerID=40&md5=d32c8660b692cf631fb930f88c6b6495},
doi = {10.1080/24750263.2022.2105964},
issn = {24750263},
year = {2022},
date = {2022-01-01},
journal = {European Zoological Journal},
volume = {89},
number = {1},
pages = {1002-1017},
publisher = {Taylor and Francis Ltd.},
abstract = {Complex biological systems often provide ready solutions for contemporary engineering. One such organism might be sponges, primitive, tissueless animals whose evolution over 600 million years has allowed them to become highly specialized. An example of such an organism is the freshwater sponge Spongilla lacustris L., an organism that filters water. This study aimed to investigate the 3D structure of the aforementioned sponge using a broad spectrum of techniques such as Microcomputed Tomography (µCT), Scanning Electron Microscopy (SEM), Confocal Laser Scanning Microscopy (CLSM), and Light Microscopy. Additionally, these techniques have been used to correlate sponge architecture with mechanical properties using the concept of tensegrity, i.e., the feature of architectural structures that self-stabilize by balancing multidirectional, often opposing, tensile and compressive forces. A more detailed look at the structure of the sponge skeleton reveals that it is based on two elements: rigid siliceous spicules, chitin in in fibres with cementing collagen-type spongin material. The coexistence of these elements in the sponge structure determines the mechanical properties and, consequently, the sponge skeleton’s postulated tensegrity. Our observations indicate that the integrity of loose megascleres is realized by sponging material surrounding the bundles of spicules. Our distinction of skeletal elements was determined by the number of spicules in the bundle, the direction of spicule position relative to the main body axis, and the way the elements were connected. The arrangement of the bundles described above has important implications for the mechanical properties of the sponge skeleton and, consequently, for the tensegrity hypothesis. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Complex biological systems often provide ready solutions for contemporary engineering. One such organism might be sponges, primitive, tissueless animals whose evolution over 600 million years has allowed them to become highly specialized. An example of such an organism is the freshwater sponge Spongilla lacustris L., an organism that filters water. This study aimed to investigate the 3D structure of the aforementioned sponge using a broad spectrum of techniques such as Microcomputed Tomography (µCT), Scanning Electron Microscopy (SEM), Confocal Laser Scanning Microscopy (CLSM), and Light Microscopy. Additionally, these techniques have been used to correlate sponge architecture with mechanical properties using the concept of tensegrity, i.e., the feature of architectural structures that self-stabilize by balancing multidirectional, often opposing, tensile and compressive forces. A more detailed look at the structure of the sponge skeleton reveals that it is based on two elements: rigid siliceous spicules, chitin in in fibres with cementing collagen-type spongin material. The coexistence of these elements in the sponge structure determines the mechanical properties and, consequently, the sponge skeleton’s postulated tensegrity. Our observations indicate that the integrity of loose megascleres is realized by sponging material surrounding the bundles of spicules. Our distinction of skeletal elements was determined by the number of spicules in the bundle, the direction of spicule position relative to the main body axis, and the way the elements were connected. The arrangement of the bundles described above has important implications for the mechanical properties of the sponge skeleton and, consequently, for the tensegrity hypothesis. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Woźnica, A.; Kwaśniewski, M.; Chwiałkowska, K.; Łozowski, B.; Absalon, D.; Libera, M.; Krzyżowski, M.; Babczyńska, A.
Microbiome dynamics modeling and analysis in relation to spatio-temporal changes in physicochemical conditions of the water ecosystem Journal Article
In: Archives of Environmental Protection, vol. 48, no. 1, pp. 18-30, 2022, ISSN: 20834772.
@article{2-s2.0-85128691739,
title = {Microbiome dynamics modeling and analysis in relation to spatio-temporal changes in physicochemical conditions of the water ecosystem},
author = { A. Woźnica and M. Kwaśniewski and K. Chwiałkowska and B. Łozowski and D. Absalon and M. Libera and M. Krzyżowski and A. Babczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128691739&doi=10.24425%2faep.2022.140542&partnerID=40&md5=62aef1f191ab748b40b4223bc7d4161e},
doi = {10.24425/aep.2022.140542},
issn = {20834772},
year = {2022},
date = {2022-01-01},
journal = {Archives of Environmental Protection},
volume = {48},
number = {1},
pages = {18-30},
publisher = {Polska Akademia Nauk},
abstract = {In this paper, we consider the development of reliable tools to assess the water quality and state of aquatic ecosystems in dynamic conditions a crucial need to address. One of such tools could be devised by monitoring the taxonomic structure of reservoirs’ microbiomes. Microbial taxa’s ecological and metabolic characteristics suggest their essential roles in maintaining the water ecosystem’s environmental equilibrium. The study aimed to explain the role of diversity and seasonal variability of the microbial communities in the ecosystem stability on the example of Goczałkowice Reservoir (Poland). The structure of the reservoir microbiome was studied using bioinformatics and modeling techniques. Water was sampled periodically in July & November 2010, and April 2011 at four representative sites. The abundance and relative fraction of the limnetic taxonomic units were determined in respect to the physicochemical indices. Significant seasonal variations in the number of operational taxonomic units (OTU) were observed within the reservoir basin’s main body but not at the main tributary’s mouth. The highest values of the correlation coefficients between OTU and physicochemical variables were obtained for Burkholderiales, Pseudoanabenales, Rickettsiales, Roseiflexales, Methylophilales, Actinomycetales, and Cryptophyta. These microorganisms are proposed as indicators of environmental conditions and water quality. Metataxonomic analyses of the freshwater microbiome in the reservoir, showed that microorganisms constitute conservative communities that undergo seasonal and local changes regarding the relative participation of the identified taxa. Therefore, we propose that monitoring those variations could provide a reliable measure of the state of aquatic ecosystems. © 2022. The Author(s).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this paper, we consider the development of reliable tools to assess the water quality and state of aquatic ecosystems in dynamic conditions a crucial need to address. One of such tools could be devised by monitoring the taxonomic structure of reservoirs’ microbiomes. Microbial taxa’s ecological and metabolic characteristics suggest their essential roles in maintaining the water ecosystem’s environmental equilibrium. The study aimed to explain the role of diversity and seasonal variability of the microbial communities in the ecosystem stability on the example of Goczałkowice Reservoir (Poland). The structure of the reservoir microbiome was studied using bioinformatics and modeling techniques. Water was sampled periodically in July & November 2010, and April 2011 at four representative sites. The abundance and relative fraction of the limnetic taxonomic units were determined in respect to the physicochemical indices. Significant seasonal variations in the number of operational taxonomic units (OTU) were observed within the reservoir basin’s main body but not at the main tributary’s mouth. The highest values of the correlation coefficients between OTU and physicochemical variables were obtained for Burkholderiales, Pseudoanabenales, Rickettsiales, Roseiflexales, Methylophilales, Actinomycetales, and Cryptophyta. These microorganisms are proposed as indicators of environmental conditions and water quality. Metataxonomic analyses of the freshwater microbiome in the reservoir, showed that microorganisms constitute conservative communities that undergo seasonal and local changes regarding the relative participation of the identified taxa. Therefore, we propose that monitoring those variations could provide a reliable measure of the state of aquatic ecosystems. © 2022. The Author(s).
2021
Francikowski, J.; Potrzebska, M.; Szulińska, E.; Tarnawska, M.; Rádai, Z.; Łozowski, B.; Baran, B.; Krzyżowski, M.
In: Scientific Reports, vol. 11, no. 1, 2021, ISSN: 20452322.
@article{2-s2.0-85117927480,
title = {The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)},
author = { J. Francikowski and M. Potrzebska and E. Szulińska and M. Tarnawska and Z. Rádai and B. Łozowski and B. Baran and M. Krzyżowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117927480&doi=10.1038%2fs41598-021-00393-5&partnerID=40&md5=1c5df52d5f93052a5bf849a3c33f4229},
doi = {10.1038/s41598-021-00393-5},
issn = {20452322},
year = {2021},
date = {2021-01-01},
journal = {Scientific Reports},
volume = {11},
number = {1},
publisher = {Nature Research},
abstract = {Tryptophan (TRP) is one of the essential amino acids in the animal body. Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level. It has been shown to have a number of essential functions, such as in the production of other biologically active molecules. The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect—house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)—white eye. This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition. A parallel goal was to compare the response of both cricket strains to HMD. ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio. Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content. A decrease in food intake was also observed in the groups on HMD. However, no changes in imago body weight and water content were observed. The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain. At the same time, a stronger detrimental effect of fructose than of glucose was apparent. Sex was found to be a modulating factor in the response to HMD. © 2021, The Author(s).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Tryptophan (TRP) is one of the essential amino acids in the animal body. Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level. It has been shown to have a number of essential functions, such as in the production of other biologically active molecules. The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect—house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)—white eye. This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition. A parallel goal was to compare the response of both cricket strains to HMD. ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio. Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content. A decrease in food intake was also observed in the groups on HMD. However, no changes in imago body weight and water content were observed. The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain. At the same time, a stronger detrimental effect of fructose than of glucose was apparent. Sex was found to be a modulating factor in the response to HMD. © 2021, The Author(s).
Krzyżowski, M.; Baran, B.; Francikowski, J.
Intergenerational transmission of resistance of callosobruchus maculatus to essential oil treatment Journal Article
In: Molecules, vol. 26, no. 15, 2021, ISSN: 14203049, (3).
@article{2-s2.0-85111617741,
title = {Intergenerational transmission of resistance of callosobruchus maculatus to essential oil treatment},
author = { M. Krzyżowski and B. Baran and J. Francikowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85111617741&doi=10.3390%2fmolecules26154541&partnerID=40&md5=c7beb40bdefa43205ef41277bcc9536c},
doi = {10.3390/molecules26154541},
issn = {14203049},
year = {2021},
date = {2021-01-01},
journal = {Molecules},
volume = {26},
number = {15},
publisher = {MDPI AG},
abstract = {Due to the rise of numerous legal restrictions as well as the increasing emergence of resistant populations, the number of available pesticides is decreasing significantly. One of the potential alternatives often described in the literature are essential oils (EOs). However, there is a lack of research addressing the potential emergence of resistance to this group of substances. In this paper, we investigated the multi-generational effects of sublethal concentrations of rosemary oil (Rosmarinus officinalis) on physiological and biochemical parameters of the cowpea weevil (Callosobruchus maculatus) such as egg laying, hatchability, oxygen consumption and acetylcholinesterase activity. Imago, which as larvae were exposed to EO at concentrations equivalent to LC25, showed significantly lower mortality. The results obtained indicate the potential development of resistance in insects exposed to EO in concentrations corresponding to LC25. In addition, in the case of the group treated with an EO concentration corresponding to LC3.12, a stimulation effect of the above-mentioned parameters was observed, which may indicate the occurrence of a hormesis effect. The obtained results may be an important reference for the development of future guidelines and EO-based insecticides. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Due to the rise of numerous legal restrictions as well as the increasing emergence of resistant populations, the number of available pesticides is decreasing significantly. One of the potential alternatives often described in the literature are essential oils (EOs). However, there is a lack of research addressing the potential emergence of resistance to this group of substances. In this paper, we investigated the multi-generational effects of sublethal concentrations of rosemary oil (Rosmarinus officinalis) on physiological and biochemical parameters of the cowpea weevil (Callosobruchus maculatus) such as egg laying, hatchability, oxygen consumption and acetylcholinesterase activity. Imago, which as larvae were exposed to EO at concentrations equivalent to LC25, showed significantly lower mortality. The results obtained indicate the potential development of resistance in insects exposed to EO in concentrations corresponding to LC25. In addition, in the case of the group treated with an EO concentration corresponding to LC3.12, a stimulation effect of the above-mentioned parameters was observed, which may indicate the occurrence of a hormesis effect. The obtained results may be an important reference for the development of future guidelines and EO-based insecticides. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
2020
Krzyżowski, M.; Baran, B.; Łozowski, B.; Francikowski, J.
The role of dilution mediums in studies of fumigant insecticidal activity of essential oils Journal Article
In: Journal of Pest Science, vol. 93, no. 4, pp. 1119-1124, 2020, ISSN: 16124758, (5).
@article{2-s2.0-85085872844,
title = {The role of dilution mediums in studies of fumigant insecticidal activity of essential oils},
author = { M. Krzyżowski and B. Baran and B. Łozowski and J. Francikowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085872844&doi=10.1007%2fs10340-020-01241-7&partnerID=40&md5=43db2bfe4f914d484e78cd9c69d81a38},
doi = {10.1007/s10340-020-01241-7},
issn = {16124758},
year = {2020},
date = {2020-01-01},
journal = {Journal of Pest Science},
volume = {93},
number = {4},
pages = {1119-1124},
publisher = {Springer},
abstract = {The essential oils (EOs) are volatile plant extracts that are commonly considered as potential biopesticides. They present promising properties as relatively safe and potent insecticides, primarily delivered via fumigation. Such specificity of the EOs has led to the growing interest of researchers and, subsequently, to an increasing body of publications. The studies focusing on assessing the insecticidal action of EOs frequently require testing the effects of several dilutions of a given EO. To this end, researchers employ a range of solvents differing widely between the studies. While informative, the standardization concerning either tested oil or pest species is lacking in these studies. The presented study herein aimed to test whether the solvent used could affect the observed insecticidal activity of tested EO. As the model setup for studying the possible influence of solvents on the insecticidal effect of EOs, two EOs: mint and rosemary were chosen. The EOs were applied in fumigation assay against three economically important species of stored product pests: Callosobruchus maculatus, Sitophilus oryzae, and Tribolium castaneum. Each EO was tested in three concentrations—diluted with a range of solvents; dimethyl sulfoxide, acetone, methanol, ethanol, paraffin (mineral) oil, ultrapure water with 0.1% polysorbate 20 (TWEEN® 20). The results confirm the hypothesis that the applied solvent could significantly alter the observed efficiency of tested EO. Such a result is of crucial value for conducting credible meta-analysis as well as for establishing a high standard of reproducibility. © 2020, The Author(s).},
note = {5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The essential oils (EOs) are volatile plant extracts that are commonly considered as potential biopesticides. They present promising properties as relatively safe and potent insecticides, primarily delivered via fumigation. Such specificity of the EOs has led to the growing interest of researchers and, subsequently, to an increasing body of publications. The studies focusing on assessing the insecticidal action of EOs frequently require testing the effects of several dilutions of a given EO. To this end, researchers employ a range of solvents differing widely between the studies. While informative, the standardization concerning either tested oil or pest species is lacking in these studies. The presented study herein aimed to test whether the solvent used could affect the observed insecticidal activity of tested EO. As the model setup for studying the possible influence of solvents on the insecticidal effect of EOs, two EOs: mint and rosemary were chosen. The EOs were applied in fumigation assay against three economically important species of stored product pests: Callosobruchus maculatus, Sitophilus oryzae, and Tribolium castaneum. Each EO was tested in three concentrations—diluted with a range of solvents; dimethyl sulfoxide, acetone, methanol, ethanol, paraffin (mineral) oil, ultrapure water with 0.1% polysorbate 20 (TWEEN® 20). The results confirm the hypothesis that the applied solvent could significantly alter the observed efficiency of tested EO. Such a result is of crucial value for conducting credible meta-analysis as well as for establishing a high standard of reproducibility. © 2020, The Author(s).
Krzyżowski, M.; Baran, B.; Łozowski, B.; Francikowski, J.
In: Insects, vol. 11, no. 6, 2020, ISSN: 20754450, (18).
@article{2-s2.0-85086038670,
title = {The effect of rosmarinus officinalis essential oil fumigation on biochemical, behavioral, and physiological parameters of callosobruchus maculatus},
author = { M. Krzyżowski and B. Baran and B. Łozowski and J. Francikowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086038670&doi=10.3390%2finsects11060344&partnerID=40&md5=a9d60d61e37ffdd900d1b75781071297},
doi = {10.3390/insects11060344},
issn = {20754450},
year = {2020},
date = {2020-01-01},
journal = {Insects},
volume = {11},
number = {6},
publisher = {MDPI AG},
abstract = {This study explores the influence of rosemary, Rosmarinus officinalis (L.) essential oil (EO) on the biochemical (acetylcholinesterase; catalase; and glutathione S-transferase), physiological (oxygen consumption), and behavioral (open field test; repellency) parameters of an important stored product insect: cowpea weevil, Callosobruchus maculatus (F.). R. officinalis EO exhibited effective insecticidal action against C. maculatus even at relatively low concentrations. LC50 = 15.69 µL/L air, and was highly repellent at concentrations equal to or above LC25. Statistically significant inhibition in locomotor activity occurred only after the acute exposure to the EO at LC12.5 and LC25. The oxygen consumption test showed metabolism increase only at LC50. An increase in activity was observed in the case of all three enzymes examined. The presented data provides a potentially valuable resource in designing more environmentally friendly and safer insecticide agents. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {18},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study explores the influence of rosemary, Rosmarinus officinalis (L.) essential oil (EO) on the biochemical (acetylcholinesterase; catalase; and glutathione S-transferase), physiological (oxygen consumption), and behavioral (open field test; repellency) parameters of an important stored product insect: cowpea weevil, Callosobruchus maculatus (F.). R. officinalis EO exhibited effective insecticidal action against C. maculatus even at relatively low concentrations. LC50 = 15.69 µL/L air, and was highly repellent at concentrations equal to or above LC25. Statistically significant inhibition in locomotor activity occurred only after the acute exposure to the EO at LC12.5 and LC25. The oxygen consumption test showed metabolism increase only at LC50. An increase in activity was observed in the case of all three enzymes examined. The presented data provides a potentially valuable resource in designing more environmentally friendly and safer insecticide agents. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Krzyżowski, M.; Francikowski, J.; Baran, B.; Babczyńska, A.
The short-chain fatty acids as potential protective agents against Callosobruchus maculatus infestation Journal Article
In: Journal of Stored Products Research, vol. 86, 2020, ISSN: 0022474X, (7).
@article{2-s2.0-85078676350,
title = {The short-chain fatty acids as potential protective agents against Callosobruchus maculatus infestation},
author = { M. Krzyżowski and J. Francikowski and B. Baran and A. Babczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078676350&doi=10.1016%2fj.jspr.2020.101570&partnerID=40&md5=13712bbc0f197facfc6c5a57afa57c5b},
doi = {10.1016/j.jspr.2020.101570},
issn = {0022474X},
year = {2020},
date = {2020-01-01},
journal = {Journal of Stored Products Research},
volume = {86},
publisher = {Elsevier Ltd},
abstract = {The cowpea weevil, Callosobruchus maculatus, is one of the most common pests of stored legumes. Its occurrence adversely affects the quality of stored beans, making them unfit for consumption, resulting in substantial financial losses. The aim of this study was to investigate the potential insecticidal properties of the volatile fatty acids (VFAs) (C1 – C5) and their influence on the insect's physiology and behavior. All VFAs in concentrations equal to 4 μl and 8 μl showed fumigant toxicity significantly higher from the control. The strongest effect was observed in the case of propionic and valeric acid in volume of 4 μl and 8 μl, where mortality was close to 100%. Except for butyric acid, all acids showed a significant repellent effect. Additionally, all VFAs significantly decreased the number of infested beans and influenced the locomotor activity. Of all tested acids only the formic acid did not affect the oxygen consumption of the insects. As the studied VFAs have noteworthy properties against C. maculatus, they could be considered as promising agents in new strategies for stored products pest management. © 2020 The Authors},
note = {7},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The cowpea weevil, Callosobruchus maculatus, is one of the most common pests of stored legumes. Its occurrence adversely affects the quality of stored beans, making them unfit for consumption, resulting in substantial financial losses. The aim of this study was to investigate the potential insecticidal properties of the volatile fatty acids (VFAs) (C1 – C5) and their influence on the insect's physiology and behavior. All VFAs in concentrations equal to 4 μl and 8 μl showed fumigant toxicity significantly higher from the control. The strongest effect was observed in the case of propionic and valeric acid in volume of 4 μl and 8 μl, where mortality was close to 100%. Except for butyric acid, all acids showed a significant repellent effect. Additionally, all VFAs significantly decreased the number of infested beans and influenced the locomotor activity. Of all tested acids only the formic acid did not affect the oxygen consumption of the insects. As the studied VFAs have noteworthy properties against C. maculatus, they could be considered as promising agents in new strategies for stored products pest management. © 2020 The Authors
2019
Krzyżowski, M.; Francikowski, J.; Baran, B.; Babczyńska, A.
Physiological and behavioral effects of different concentrations of diatomaceous earth on common stored product pest Callosobruchus maculatus Journal Article
In: Journal of Stored Products Research, vol. 82, pp. 110-115, 2019, ISSN: 0022474X, (8).
@article{2-s2.0-85066086872,
title = {Physiological and behavioral effects of different concentrations of diatomaceous earth on common stored product pest Callosobruchus maculatus},
author = { M. Krzyżowski and J. Francikowski and B. Baran and A. Babczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066086872&doi=10.1016%2fj.jspr.2019.05.004&partnerID=40&md5=463e91e3a5a4fb5331b73f703795bed5},
doi = {10.1016/j.jspr.2019.05.004},
issn = {0022474X},
year = {2019},
date = {2019-01-01},
journal = {Journal of Stored Products Research},
volume = {82},
pages = {110-115},
publisher = {Elsevier Ltd},
abstract = {The presented research focuses on the assessment of the changes in the behavioral and physiological parameters of Callosobruchus maculatus (F.)(Coleoptera: Chrysomelidae), treated with amorphous diatomaceous earth (DE)(Perma- Guard™). During the experiments, parameters such as stimulated and spontaneous locomotor activity, oxygen consumption and fecundity were tested. Increasing DE concentration caused significant inhibition in both oxygen consumption and locomotor activity. Moreover, the treated insects had significantly lower fecundity, due to decreased egg laying, lower number of infested beans as well as lowered hatchability. By limiting the progeny number, DE possibly prevents or at least reduces the size of the C. maculatus outbreak. Additionally, scanning electron microscope (SEM)micrographs revealed that DE particles accumulate especially in joints or hairy regions of the insect body close to the sensilla. This, along with data on locomotor activity, indicates a possible presence of additional, previously undescribed, mode of DE action - as an irritant. In conclusion, the presented results contribute to the understanding of the insecticidal activity of DE and provide novel data on the potential application of oxygen consumption as a pesticide efficiency marker. © 2019 Elsevier Ltd},
note = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The presented research focuses on the assessment of the changes in the behavioral and physiological parameters of Callosobruchus maculatus (F.)(Coleoptera: Chrysomelidae), treated with amorphous diatomaceous earth (DE)(Perma- Guard™). During the experiments, parameters such as stimulated and spontaneous locomotor activity, oxygen consumption and fecundity were tested. Increasing DE concentration caused significant inhibition in both oxygen consumption and locomotor activity. Moreover, the treated insects had significantly lower fecundity, due to decreased egg laying, lower number of infested beans as well as lowered hatchability. By limiting the progeny number, DE possibly prevents or at least reduces the size of the C. maculatus outbreak. Additionally, scanning electron microscope (SEM)micrographs revealed that DE particles accumulate especially in joints or hairy regions of the insect body close to the sensilla. This, along with data on locomotor activity, indicates a possible presence of additional, previously undescribed, mode of DE action - as an irritant. In conclusion, the presented results contribute to the understanding of the insecticidal activity of DE and provide novel data on the potential application of oxygen consumption as a pesticide efficiency marker. © 2019 Elsevier Ltd
Francikowski, J.; Krzyżowski, M.; Kochańska, B.; Potrzebska, M.; Baran, B.; Chajec, Ł.; Urbisz, A. Z.; Małota, K.; Łozowski, B.; Kloc, M.; Kubiak, J.
Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus Journal Article
In: PLoS ONE, vol. 14, no. 5, 2019, ISSN: 19326203, (5).
@article{2-s2.0-85065662274,
title = {Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus},
author = { J. Francikowski and M. Krzyżowski and B. Kochańska and M. Potrzebska and B. Baran and Ł. Chajec and A.Z. Urbisz and K. Małota and B. Łozowski and M. Kloc and J. Kubiak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065662274&doi=10.1371%2fJOURNAL.PONE.0216281&partnerID=40&md5=f7c1822cf373b0ac1bceaf810a30d257},
doi = {10.1371/JOURNAL.PONE.0216281},
issn = {19326203},
year = {2019},
date = {2019-01-01},
journal = {PLoS ONE},
volume = {14},
number = {5},
publisher = {Public Library of Science},
abstract = {Two eye-colour mutant strains, white (W) and yellow (Y) of house cricket Acheta domesticus were established in our laboratory. We phenotyped and genotyped the mutants, performed genetic crossings and studied the eye structure and pigment composition using light and electron microscopy and biochemical analysis. We show that W and Y phenotypes are controlled by a single autosomal recessive allele, as both traits are metabolically independent. The analysis of the mutants'eye structure showed a reduced number of dark pigment granules while simultaneously, and an increased amount of light vacuoles in white eye mutants was observed. Significant differences in eye pigment composition between strains were also found. The Y mutant had a lower number of ommochromes, while the W mutant had a lower number of ommochromes and pteridines. This indicates that mutated genes are involved in two different, independent metabolic pathways regulating tryptophan metabolism enzymes, pigment transporter granules or pigment granule formation. Copyright: © 2019 Francikowski et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},
note = {5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Two eye-colour mutant strains, white (W) and yellow (Y) of house cricket Acheta domesticus were established in our laboratory. We phenotyped and genotyped the mutants, performed genetic crossings and studied the eye structure and pigment composition using light and electron microscopy and biochemical analysis. We show that W and Y phenotypes are controlled by a single autosomal recessive allele, as both traits are metabolically independent. The analysis of the mutants'eye structure showed a reduced number of dark pigment granules while simultaneously, and an increased amount of light vacuoles in white eye mutants was observed. Significant differences in eye pigment composition between strains were also found. The Y mutant had a lower number of ommochromes, while the W mutant had a lower number of ommochromes and pteridines. This indicates that mutated genes are involved in two different, independent metabolic pathways regulating tryptophan metabolism enzymes, pigment transporter granules or pigment granule formation. Copyright: © 2019 Francikowski et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Tarnawska, M.; Augustyniak, M.; Łaszczyca, P.; Migula, P.; Irnazarow, I.; Krzyżowski, M.; Babczyńska, A.
Immune response of juvenile common carp (Cyprinus carpio L.) exposed to a mixture of sewage chemicals Journal Article
In: Fish and Shellfish Immunology, vol. 88, pp. 17-27, 2019, ISSN: 10504648, (6).
@article{2-s2.0-85062391000,
title = {Immune response of juvenile common carp (Cyprinus carpio L.) exposed to a mixture of sewage chemicals},
author = { M. Tarnawska and M. Augustyniak and P. Łaszczyca and P. Migula and I. Irnazarow and M. Krzyżowski and A. Babczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062391000&doi=10.1016%2fj.fsi.2019.02.049&partnerID=40&md5=d42712874f989fa17f5c77ff077e7bc5},
doi = {10.1016/j.fsi.2019.02.049},
issn = {10504648},
year = {2019},
date = {2019-01-01},
journal = {Fish and Shellfish Immunology},
volume = {88},
pages = {17-27},
publisher = {Academic Press},
abstract = {Pharmaceuticals and household chemicals are important components of municipal sewage. Many of them are biologically active, disrupting not only hormonal regulation of aquatic animals but also, indirectly, disturbing their immunological protection. In the environment, chemicals rarely act as individual substances, but as elements of mixtures. Therefore, the aim of this study was to check whether the acute laboratory exposure of common carp juveniles to a mixture of ibuprofen, sodium dodecyl sulphate (SDS), dimethyl sulfoxide (DMSO) and 17 α-ethynylestradiol in increasing concentrations, modifies the levels of innate immunity (lysozyme; C-reactive protein) as well as general stress (metallothioneins; heat shock proteins HSP70) markers in brain, liver, gills, spleen and mucus. The levels of the markers were measured by an immunodetection technique. Not only do the pharmaceuticals and household chemicals impair immunological reactions of young carp in various tissues but also do that in a concentration-dependent manner in the liver, gills, spleen and mucus. This has a very important implication, since it may result in higher sensitivity of young fish to pathogens due to energy allocation to defence processes. The comparisons of the pattern of stress reactions in the studied organ samples indicated that mucus appeared to be a good, non-invasive material for monitoring of environmental state and fish conditions. © 2019},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Pharmaceuticals and household chemicals are important components of municipal sewage. Many of them are biologically active, disrupting not only hormonal regulation of aquatic animals but also, indirectly, disturbing their immunological protection. In the environment, chemicals rarely act as individual substances, but as elements of mixtures. Therefore, the aim of this study was to check whether the acute laboratory exposure of common carp juveniles to a mixture of ibuprofen, sodium dodecyl sulphate (SDS), dimethyl sulfoxide (DMSO) and 17 α-ethynylestradiol in increasing concentrations, modifies the levels of innate immunity (lysozyme; C-reactive protein) as well as general stress (metallothioneins; heat shock proteins HSP70) markers in brain, liver, gills, spleen and mucus. The levels of the markers were measured by an immunodetection technique. Not only do the pharmaceuticals and household chemicals impair immunological reactions of young carp in various tissues but also do that in a concentration-dependent manner in the liver, gills, spleen and mucus. This has a very important implication, since it may result in higher sensitivity of young fish to pathogens due to energy allocation to defence processes. The comparisons of the pattern of stress reactions in the studied organ samples indicated that mucus appeared to be a good, non-invasive material for monitoring of environmental state and fish conditions. © 2019
Francikowski, J.; Baran, B.; Cup, M.; Janiec, J.; Krzyżowski, M.
Commercially available essential oil formulas as repellents against the stored-product pest Alphitobius diaperinus Journal Article
In: Insects, vol. 10, no. 4, 2019, ISSN: 20754450, (13).
@article{2-s2.0-85067617560,
title = {Commercially available essential oil formulas as repellents against the stored-product pest Alphitobius diaperinus},
author = { J. Francikowski and B. Baran and M. Cup and J. Janiec and M. Krzyżowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067617560&doi=10.3390%2finsects10040096&partnerID=40&md5=c363c642e10755a72da43ff5ca1a11c9},
doi = {10.3390/insects10040096},
issn = {20754450},
year = {2019},
date = {2019-01-01},
journal = {Insects},
volume = {10},
number = {4},
publisher = {MDPI AG},
abstract = {The main aim of the presented paper is to assess the potential repellent effect of selected essential oils (EOs) against the lesser mealworm (Alphitobius diaperinus), which can cause economic losses in storage and in the poultry industry. Due to the development of pesticide resistance in A. diaperinus populations, as well as an attempt to limit extensive use of potentially harmful pesticides in food-related industries, there is a strong need for the development of alternative methods of dealing with A. diaperinus infestations. Because of their cost-effectiveness, availability and low vertebrate toxicity, EOs are promising agents in pest management. In the presented paper four off-the-shelf EOs: Mint, vanilla, lemon and citronella (and mixtures of them) were tested as potential repellents. Moreover, a novel preference assay, providing an extended analysis of the preference and the locomotor response, was used. The most effective EOs were: Citronella and lemon. EOs mixtures were generally more repellent than individual EOs, with the lemon and vanilla 1:1 mixture acting as the strongest repellent. A few of the tested EOs caused significant alterations to the locomotor activity, although no direct relation was observed. In conclusion, EOs can be potentially used as repellent agents in A. diaperinus management. Additionally, data on the locomotor activity may lead to designing better push-pull strategies in pest management. © 2019 by the authors. Licensee MDPI, Basel, Switzerland},
note = {13},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The main aim of the presented paper is to assess the potential repellent effect of selected essential oils (EOs) against the lesser mealworm (Alphitobius diaperinus), which can cause economic losses in storage and in the poultry industry. Due to the development of pesticide resistance in A. diaperinus populations, as well as an attempt to limit extensive use of potentially harmful pesticides in food-related industries, there is a strong need for the development of alternative methods of dealing with A. diaperinus infestations. Because of their cost-effectiveness, availability and low vertebrate toxicity, EOs are promising agents in pest management. In the presented paper four off-the-shelf EOs: Mint, vanilla, lemon and citronella (and mixtures of them) were tested as potential repellents. Moreover, a novel preference assay, providing an extended analysis of the preference and the locomotor response, was used. The most effective EOs were: Citronella and lemon. EOs mixtures were generally more repellent than individual EOs, with the lemon and vanilla 1:1 mixture acting as the strongest repellent. A few of the tested EOs caused significant alterations to the locomotor activity, although no direct relation was observed. In conclusion, EOs can be potentially used as repellent agents in A. diaperinus management. Additionally, data on the locomotor activity may lead to designing better push-pull strategies in pest management. © 2019 by the authors. Licensee MDPI, Basel, Switzerland
2018
Baran, B.; Krzyżowski, M.; Cup, M.; Janiec, J.; Grabowski, M.; Francikowski, J.
Repellent effect of volatile fatty acids on lesser mealworm (Alphitobius diaperinus) Journal Article
In: Insects, vol. 9, no. 1, 2018, ISSN: 20754450, (6).
@article{2-s2.0-85046398169,
title = {Repellent effect of volatile fatty acids on lesser mealworm (Alphitobius diaperinus)},
author = { B. Baran and M. Krzyżowski and M. Cup and J. Janiec and M. Grabowski and J. Francikowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046398169&doi=10.3390%2finsects9010035&partnerID=40&md5=b6f2d6ebe02b9a7a359441cb092e3ac1},
doi = {10.3390/insects9010035},
issn = {20754450},
year = {2018},
date = {2018-01-01},
journal = {Insects},
volume = {9},
number = {1},
publisher = {MDPI AG},
abstract = {Volatile fatty acids (VFAs) are a group of common metabolites and semiochemicals mediating information transfer between higher organisms and bacteria, either from microbiome or external environment. VFAs commonly occur among various insect orders. There are numerous studies exploring their influence on the behavior of different insect species. In relation to the papers published by J. E. McFarlane in 1985, we assessed the effects of formic, acetic, propionic, butyric and valeric acids on the spatial preference of the lesser mealworm (Alphitobius diaperinus), a common pest of stored food grain products and the poultry industry. The main aim of the presented study was to provide new angles in VFA research, recreating the classical study both with new methods and on economically significant pest species. This paper presents a novel method of continuous, simultaneous assessment of site preference and the travelled distance in a constant-flow olfactometer. All the tested VFAs, except valeric acid, had a significant repellent effect, with formic acid being effective even at the lowest used concentration. Additionally, the VFAs significantly altered the distance travelled by the insects. The obtained results indicate a potential role for VFAs in the olfactory guided behavior of A. diaperinus. It is suspected that the reaction to the presence of VFAs may deviate from the specificity of species’ original habitat. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Volatile fatty acids (VFAs) are a group of common metabolites and semiochemicals mediating information transfer between higher organisms and bacteria, either from microbiome or external environment. VFAs commonly occur among various insect orders. There are numerous studies exploring their influence on the behavior of different insect species. In relation to the papers published by J. E. McFarlane in 1985, we assessed the effects of formic, acetic, propionic, butyric and valeric acids on the spatial preference of the lesser mealworm (Alphitobius diaperinus), a common pest of stored food grain products and the poultry industry. The main aim of the presented study was to provide new angles in VFA research, recreating the classical study both with new methods and on economically significant pest species. This paper presents a novel method of continuous, simultaneous assessment of site preference and the travelled distance in a constant-flow olfactometer. All the tested VFAs, except valeric acid, had a significant repellent effect, with formic acid being effective even at the lowest used concentration. Additionally, the VFAs significantly altered the distance travelled by the insects. The obtained results indicate a potential role for VFAs in the olfactory guided behavior of A. diaperinus. It is suspected that the reaction to the presence of VFAs may deviate from the specificity of species’ original habitat. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
2017
Hohol, M.; Baran, B.; Krzyżowski, M.; Francikowski, J.
Does spatial navigation have a blind-spot? Visiocentrism is not enough to explain the navigational behavior comprehensively Journal Article
In: Frontiers in Behavioral Neuroscience, vol. 11, 2017, ISSN: 16625153, (7).
@article{2-s2.0-85027884159,
title = {Does spatial navigation have a blind-spot? Visiocentrism is not enough to explain the navigational behavior comprehensively},
author = { M. Hohol and B. Baran and M. Krzyżowski and J. Francikowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027884159&doi=10.3389%2ffnbeh.2017.00154&partnerID=40&md5=bd9645a2152a3a63770065439c8aba9e},
doi = {10.3389/fnbeh.2017.00154},
issn = {16625153},
year = {2017},
date = {2017-01-01},
journal = {Frontiers in Behavioral Neuroscience},
volume = {11},
publisher = {Frontiers Media S.A.},
abstract = {[No abstract available]},
note = {7},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
[No abstract available]
2016
Francikowski, J.; Baran, B.; Płachetka-Bożek, A.; Krzyżowski, M.; Augustyniak, M.
Caffeine effects on AdoR mRNA expression in Drosophila melanogaster Journal Article
In: Open Life Sciences, vol. 11, no. 1, pp. 244-249, 2016, ISSN: 23915412, (1).
@article{2-s2.0-84994000474,
title = {Caffeine effects on AdoR mRNA expression in Drosophila melanogaster},
author = { J. Francikowski and B. Baran and A. Płachetka-Bożek and M. Krzyżowski and M. Augustyniak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84994000474&doi=10.1515%2fbiol-2016-0034&partnerID=40&md5=4ed7fa379ebd2558062e919116cadecf},
doi = {10.1515/biol-2016-0034},
issn = {23915412},
year = {2016},
date = {2016-01-01},
journal = {Open Life Sciences},
volume = {11},
number = {1},
pages = {244-249},
publisher = {De Gruyter Open Ltd},
abstract = {In this study, we aimed to evaluate whether exposure to caffeine in the early stages of development affect AdoR mRNA expression levels in the fruit fly (Drosophila melanogaster) and how this will relate to the developmental success of flies. Adenosine receptors are seen as the most important biochemical targets of caffeine. Simultaneously adenosine signaling orchestrates the development and growth of insects. We demonstrate that AdoR mRNA expression in D. melanogaster is persistent from early stages till imago. Strong alterations in AdoR expression were observed in larvae that had been treated with caffeine. However, after the imaginal molt, the differences in AdoR expression between the insects from all of the test groups evened out despite a wide range of developmental success in the groups. Taken together, these results suggest that caffeine affects the expression of its cellular targets even from the early stages of fruit fly development and thus there is a significantly lower larvae-to-adult survival rate. Moreover, we also proved that the expression of AdoR undergoes a peculiar reset during the maturation of D. melanogaster despite the conditions in which larvae developed. © 2016 Jacek Francikowski et al.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this study, we aimed to evaluate whether exposure to caffeine in the early stages of development affect AdoR mRNA expression levels in the fruit fly (Drosophila melanogaster) and how this will relate to the developmental success of flies. Adenosine receptors are seen as the most important biochemical targets of caffeine. Simultaneously adenosine signaling orchestrates the development and growth of insects. We demonstrate that AdoR mRNA expression in D. melanogaster is persistent from early stages till imago. Strong alterations in AdoR expression were observed in larvae that had been treated with caffeine. However, after the imaginal molt, the differences in AdoR expression between the insects from all of the test groups evened out despite a wide range of developmental success in the groups. Taken together, these results suggest that caffeine affects the expression of its cellular targets even from the early stages of fruit fly development and thus there is a significantly lower larvae-to-adult survival rate. Moreover, we also proved that the expression of AdoR undergoes a peculiar reset during the maturation of D. melanogaster despite the conditions in which larvae developed. © 2016 Jacek Francikowski et al.