• dr Małgorzata Nawrot
Stanowisko: St. spec. inż.-techn.
Jednostka: Biuro ds. Infrastruktury Badawczo-Dydaktycznej WNP
Adres: 40-032 Katowice, ul. Jagiellońska 28
Piętro: II
Numer pokoju: C-249
Telefon: (32) 2009 562
E-mail: malgorzata.nawrot@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 7004343637
Publikacje z bazy Scopus
2025
Szurman-Zubrzycka, M. E.; Kocjan, A.; Spałek, E.; Gajecka, M.; Jędrzejek, P.; Nawrot, M.; Szarejko, I.; Kwaśniewska, J.
To divide or not to divide? NAC8 (SOG1) as a key regulator of DNA damage response in barley (Hordeum vulgare L.) Journal Article
In: DNA Repair, vol. 146, 2025, ISSN: 15687864, (0).
@article{2-s2.0-85217713663,
title = {To divide or not to divide? NAC8 (SOG1) as a key regulator of DNA damage response in barley (Hordeum vulgare L.)},
author = { M.E. Szurman-Zubrzycka and A. Kocjan and E. Spałek and M. Gajecka and P. Jędrzejek and M. Nawrot and I. Szarejko and J. Kwaśniewska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85217713663&doi=10.1016%2fj.dnarep.2025.103810&partnerID=40&md5=5e3bacb7fa861c5fe7e7014ee926f570},
doi = {10.1016/j.dnarep.2025.103810},
issn = {15687864},
year = {2025},
date = {2025-01-01},
journal = {DNA Repair},
volume = {146},
publisher = {Elsevier B.V.},
abstract = {We identified several new TILLING mutants of barley (Hordeum vulgare L.) with missense mutations in the HvNAC8 gene, a homolog of the SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1) gene in Arabidopsis thaliana. In Arabidopsis, SOG1 is the primary regulator of the DNA Damage Response (DDR) pathway. We aimed to transfer this knowledge to barley, an agriculturally important crop. Our detailed analysis of the hvnac8.k mutant revealed an impaired DDR pathway. The hvnac8.k mutant accumulates DNA damage under genotoxic stress induced by zeocin, but it also shows increased DNA damage under normal growth conditions. Despite this, the frequency of dividing cells in the root meristem of the mutant treated with zeocin is much less affected than in the wild type. This suggests that the mutant bypasses the typical DDR regulation, where cell division is halted to allow DNA repair following damage. We also analyzed our mutant under aluminum (Al³⁺) stress. Aluminum ions, present in acidic soils that constitute approximately 50 % of arable land, are a common stressor that significantly reduce barley yield. Al³ ⁺ is known to cause DNA damage and activate DDR. Consequently, we aimed to assess whether the hvnac8.k phenotype could confer a beneficial effect under aluminum stress, a widespread agronomic challenge. Our findings suggest that modulation of the DDR pathway has the potential to improve aluminum tolerance in barley. © 2025 The Authors},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We identified several new TILLING mutants of barley (Hordeum vulgare L.) with missense mutations in the HvNAC8 gene, a homolog of the SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1) gene in Arabidopsis thaliana. In Arabidopsis, SOG1 is the primary regulator of the DNA Damage Response (DDR) pathway. We aimed to transfer this knowledge to barley, an agriculturally important crop. Our detailed analysis of the hvnac8.k mutant revealed an impaired DDR pathway. The hvnac8.k mutant accumulates DNA damage under genotoxic stress induced by zeocin, but it also shows increased DNA damage under normal growth conditions. Despite this, the frequency of dividing cells in the root meristem of the mutant treated with zeocin is much less affected than in the wild type. This suggests that the mutant bypasses the typical DDR regulation, where cell division is halted to allow DNA repair following damage. We also analyzed our mutant under aluminum (Al³⁺) stress. Aluminum ions, present in acidic soils that constitute approximately 50 % of arable land, are a common stressor that significantly reduce barley yield. Al³ ⁺ is known to cause DNA damage and activate DDR. Consequently, we aimed to assess whether the hvnac8.k phenotype could confer a beneficial effect under aluminum stress, a widespread agronomic challenge. Our findings suggest that modulation of the DDR pathway has the potential to improve aluminum tolerance in barley. © 2025 The Authors
2021
Szurman-Zubrzycka, M. E.; Chwiałkowska, K.; Niemira, M.; Kwaśniewski, M.; Nawrot, M.; Gajecka, M.; Larsen, P. B.; Szarejko, I.
Aluminum or Low pH – Which Is the Bigger Enemy of Barley? Transcriptome Analysis of Barley Root Meristem Under Al and Low pH Stress Journal Article
In: Frontiers in Genetics, vol. 12, 2021, ISSN: 16648021, (4).
@article{2-s2.0-85107199912,
title = {Aluminum or Low pH – Which Is the Bigger Enemy of Barley? Transcriptome Analysis of Barley Root Meristem Under Al and Low pH Stress},
author = { M.E. Szurman-Zubrzycka and K. Chwiałkowska and M. Niemira and M. Kwaśniewski and M. Nawrot and M. Gajecka and P.B. Larsen and I. Szarejko},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107199912&doi=10.3389%2ffgene.2021.675260&partnerID=40&md5=74ea8683f454a79bd22287d33605b161},
doi = {10.3389/fgene.2021.675260},
issn = {16648021},
year = {2021},
date = {2021-01-01},
journal = {Frontiers in Genetics},
volume = {12},
publisher = {Frontiers Media S.A.},
abstract = {Aluminum (Al) toxicity is considered to be the most harmful abiotic stress in acidic soils that today comprise more than 50% of the world’s arable lands. Barley belongs to a group of crops that are most sensitive to Al in low pH soils. We present the RNA-seq analysis of root meristems of barley seedlings grown in hydroponics at optimal pH (6.0), low pH (4.0), and low pH with Al (10 μM of bioavailable Al3+ ions). Two independent experiments were conducted: with short-term (24 h) and long-term (7 days) Al treatment. In the short-term experiment, more genes were differentially expressed (DEGs) between root meristems grown at pH = 6.0 and pH = 4.0, than between those grown at pH = 4.0 with and without Al treatment. The genes upregulated by low pH were associated mainly with response to oxidative stress, cell wall organization, and iron ion binding. Among genes upregulated by Al, overrepresented were those related to response to stress condition and calcium ion binding. In the long-term experiment, the number of DEGs between hydroponics at pH = 4.0 and 6.0 were lower than in the short-term experiment, which suggests that plants partially adapted to the low pH. Interestingly, 7 days Al treatment caused massive changes in the transcriptome profile. Over 4,000 genes were upregulated and almost 2,000 genes were downregulated by long-term Al stress. These DEGs were related to stress response, cell wall development and metal ion transport. Based on our results we can assume that both, Al3+ ions and low pH are harmful to barley plants. Additionally, we phenotyped the root system of barley seedlings grown in the same hydroponic conditions for 7 days at pH = 6.0},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Aluminum (Al) toxicity is considered to be the most harmful abiotic stress in acidic soils that today comprise more than 50% of the world’s arable lands. Barley belongs to a group of crops that are most sensitive to Al in low pH soils. We present the RNA-seq analysis of root meristems of barley seedlings grown in hydroponics at optimal pH (6.0), low pH (4.0), and low pH with Al (10 μM of bioavailable Al3+ ions). Two independent experiments were conducted: with short-term (24 h) and long-term (7 days) Al treatment. In the short-term experiment, more genes were differentially expressed (DEGs) between root meristems grown at pH = 6.0 and pH = 4.0, than between those grown at pH = 4.0 with and without Al treatment. The genes upregulated by low pH were associated mainly with response to oxidative stress, cell wall organization, and iron ion binding. Among genes upregulated by Al, overrepresented were those related to response to stress condition and calcium ion binding. In the long-term experiment, the number of DEGs between hydroponics at pH = 4.0 and 6.0 were lower than in the short-term experiment, which suggests that plants partially adapted to the low pH. Interestingly, 7 days Al treatment caused massive changes in the transcriptome profile. Over 4,000 genes were upregulated and almost 2,000 genes were downregulated by long-term Al stress. These DEGs were related to stress response, cell wall development and metal ion transport. Based on our results we can assume that both, Al3+ ions and low pH are harmful to barley plants. Additionally, we phenotyped the root system of barley seedlings grown in the same hydroponic conditions for 7 days at pH = 6.0
2019
Szurman-Zubrzycka, M. E.; Nawrot, M.; Jelonek, J.; Dziekanowski, M.; Kwaśniewska, J.; Szarejko, I.
ATR, a DNA Damage Signaling Kinase, Is Involved in Aluminum Response in Barley Journal Article
In: Frontiers in Plant Science, vol. 10, 2019, ISSN: 1664462X, (5).
@article{2-s2.0-85074643338,
title = {ATR, a DNA Damage Signaling Kinase, Is Involved in Aluminum Response in Barley},
author = { M.E. Szurman-Zubrzycka and M. Nawrot and J. Jelonek and M. Dziekanowski and J. Kwaśniewska and I. Szarejko},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074643338&doi=10.3389%2ffpls.2019.01299&partnerID=40&md5=0b22bc4166d9f0fff9b0eacbdcc23881},
doi = {10.3389/fpls.2019.01299},
issn = {1664462X},
year = {2019},
date = {2019-01-01},
journal = {Frontiers in Plant Science},
volume = {10},
publisher = {Frontiers Media S.A.},
abstract = {Ataxia Telangiectasia and Rad-3-related protein (ATR) is a DNA damage signaling kinase required for the monitoring of DNA integrity. Together with ATM and SOG1, it is a key player in the transcriptional regulation of DNA damage response (DDR) genes in plants. In this study, we describe the role of ATR in the DDR pathway in barley and the function of the HvATR gene in response to DNA damages induced by aluminum toxicity. Aluminum is the third most abundant element in the Earth’s crust. It becomes highly phytotoxic in acidic soils, which comprise more than 50% of arable lands worldwide. At low pH, Al is known to be a genotoxic agent causing DNA damage and cell cycle arrest. We present barley mutants, hvatr.g and hvatr.i, developed by TILLING strategy. The hvatr.g mutant carries a G6054A missense mutation in the ATR gene, leading to the substitution of a highly conserved amino acid in the protein (G1015S). The hvatr.g mutant showed the impaired DDR pathway. It accumulated DNA damages in the nuclei of root meristem cells when grown in control conditions. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) analysis revealed that 60% of mutant nuclei possessed DNA nicks and breaks, whereas in the wild type only 2% of the nuclei were TUNEL-positive. The high frequency of DNA damages did not lead to the inhibition of the cell cycle progression, but the mutant showed an increased number of cells in the G2/M phase. In response to treatments with different Al doses, hvatr.g showed a high level of tolerance. The retention of root growth, which is the most evident symptom of Al toxicity, was not observed in the mutant, as it was in its parent variety. Furthermore, Al treatment increased the level of DNA damages, but did not affect the mitotic activity and the cell cycle profile in the hvatr.g mutant. A similar phenotype was observed for the hvatr.i mutant, carrying another missense mutation leading to G903E substitution in the HvATR protein. Our results demonstrate that the impaired mechanism of DNA damage response may lead to aluminum tolerance. They shed a new light on the role of the ATR-dependent DDR pathway in an agronomically important species. © Copyright © 2019 Szurman-Zubrzycka, Nawrot, Jelonek, Dziekanowski, Kwasniewska and Szarejko.},
note = {5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Ataxia Telangiectasia and Rad-3-related protein (ATR) is a DNA damage signaling kinase required for the monitoring of DNA integrity. Together with ATM and SOG1, it is a key player in the transcriptional regulation of DNA damage response (DDR) genes in plants. In this study, we describe the role of ATR in the DDR pathway in barley and the function of the HvATR gene in response to DNA damages induced by aluminum toxicity. Aluminum is the third most abundant element in the Earth’s crust. It becomes highly phytotoxic in acidic soils, which comprise more than 50% of arable lands worldwide. At low pH, Al is known to be a genotoxic agent causing DNA damage and cell cycle arrest. We present barley mutants, hvatr.g and hvatr.i, developed by TILLING strategy. The hvatr.g mutant carries a G6054A missense mutation in the ATR gene, leading to the substitution of a highly conserved amino acid in the protein (G1015S). The hvatr.g mutant showed the impaired DDR pathway. It accumulated DNA damages in the nuclei of root meristem cells when grown in control conditions. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) analysis revealed that 60% of mutant nuclei possessed DNA nicks and breaks, whereas in the wild type only 2% of the nuclei were TUNEL-positive. The high frequency of DNA damages did not lead to the inhibition of the cell cycle progression, but the mutant showed an increased number of cells in the G2/M phase. In response to treatments with different Al doses, hvatr.g showed a high level of tolerance. The retention of root growth, which is the most evident symptom of Al toxicity, was not observed in the mutant, as it was in its parent variety. Furthermore, Al treatment increased the level of DNA damages, but did not affect the mitotic activity and the cell cycle profile in the hvatr.g mutant. A similar phenotype was observed for the hvatr.i mutant, carrying another missense mutation leading to G903E substitution in the HvATR protein. Our results demonstrate that the impaired mechanism of DNA damage response may lead to aluminum tolerance. They shed a new light on the role of the ATR-dependent DDR pathway in an agronomically important species. © Copyright © 2019 Szurman-Zubrzycka, Nawrot, Jelonek, Dziekanowski, Kwasniewska and Szarejko.
2016
Szarejko, I.; Szurman-Zubrzycka, M. E.; Nawrot, M.; Marzec, M.; Gruszka, D.; Kurowska, M. M.; Chmielewska, B.; Zbieszczyk, J.; Jelonek, J.; Maluszynski, M.
Creation of a TILLING population in barley after chemical mutagenesis with sodium azide and MNU Book Chapter
In: pp. 91-111, Springer International Publishing, 2016, ISBN: 9783319450216; 9783319450193, (13).
@inbook{2-s2.0-85021060188,
title = {Creation of a TILLING population in barley after chemical mutagenesis with sodium azide and MNU},
author = { I. Szarejko and M.E. Szurman-Zubrzycka and M. Nawrot and M. Marzec and D. Gruszka and M.M. Kurowska and B. Chmielewska and J. Zbieszczyk and J. Jelonek and M. Maluszynski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021060188&doi=10.1007%2f978-3-319-45021-6_6&partnerID=40&md5=769f75988eadba3f52354d86c8d9403e},
doi = {10.1007/978-3-319-45021-6_6},
isbn = {9783319450216; 9783319450193},
year = {2016},
date = {2016-01-01},
journal = {Biotechnologies for Plant Mutation Breeding: Protocols},
pages = {91-111},
publisher = {Springer International Publishing},
abstract = {Since the development of the Targeting Induced Local Lesions in Genome (TILLING) strategy, it has been applied in both plants and animals in many studies. The creation of an appropriate population is the first and most crucial step of TILLING. The goal is to obtain a highly mutagenized population that allows many mutations in any gene of interest to be found. Therefore, an effective method of mutation induction should be developed. A high mutation density is associated with saving time, costs, and the labor required for the development of a TILLING platform. The proper handling of the mutated generations, the establishment of a seed bank, and the development of a DNA library are essential for creating a TILLING population. The database in which all of the data from the molecular and phenotypic analyses are collected is a very useful tool for maintaining such population. Once developed, a TILLING population can serve as a renewable resource of mutations for research that uses both forward and reverse genetic approaches. In this chapter, we describe the methods for the development and maintenance of a TILLING population in barley. © International Atomic Energy Agency 2017.},
note = {13},
keywords = {},
pubstate = {published},
tppubtype = {inbook}
}
Since the development of the Targeting Induced Local Lesions in Genome (TILLING) strategy, it has been applied in both plants and animals in many studies. The creation of an appropriate population is the first and most crucial step of TILLING. The goal is to obtain a highly mutagenized population that allows many mutations in any gene of interest to be found. Therefore, an effective method of mutation induction should be developed. A high mutation density is associated with saving time, costs, and the labor required for the development of a TILLING platform. The proper handling of the mutated generations, the establishment of a seed bank, and the development of a DNA library are essential for creating a TILLING population. The database in which all of the data from the molecular and phenotypic analyses are collected is a very useful tool for maintaining such population. Once developed, a TILLING population can serve as a renewable resource of mutations for research that uses both forward and reverse genetic approaches. In this chapter, we describe the methods for the development and maintenance of a TILLING population in barley. © International Atomic Energy Agency 2017.
2014
Chmielewska, B.; Janiak, A.; Karcz, J.; Guzy-Wróbelska, J.; Forster, B. P.; Nawrot, M.; Rusek, A.; Smyda, P.; Kędziorski, P.; Maluszynski, M.; Szarejko, I.
Morphological, genetic and molecular characteristics of barley root hair mutants Journal Article
In: Journal of Applied Genetics, vol. 55, no. 4, pp. 433-447, 2014, ISSN: 12341983, (14).
@article{2-s2.0-84927173271,
title = {Morphological, genetic and molecular characteristics of barley root hair mutants},
author = { B. Chmielewska and A. Janiak and J. Karcz and J. Guzy-Wróbelska and B.P. Forster and M. Nawrot and A. Rusek and P. Smyda and P. Kędziorski and M. Maluszynski and I. Szarejko},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927173271&doi=10.1007%2fs13353-014-0225-x&partnerID=40&md5=6c3835a594fbf5122995a378c846e7ad},
doi = {10.1007/s13353-014-0225-x},
issn = {12341983},
year = {2014},
date = {2014-01-01},
journal = {Journal of Applied Genetics},
volume = {55},
number = {4},
pages = {433-447},
publisher = {Springer Verlag},
abstract = {Root hairs are tubular outgrowths of specialized epidermal cells called trichoblasts. They affect anchoring plants in soil, the uptake of water and nutrients and are the sites of the interaction between plants and microorganisms. Nineteen root hair mutants of barley representing different stages of root hair development were subjected to detailed morphological and genetic analyses. Each mutant was monogenic and recessive. An allelism test revealed that nine loci were responsible for the mutated root hair phenotypes in the collection and 1–4 mutated allelic forms were identified at each locus. Genetic relationships between the genes responsible for different stages of root hair formation were established. The linkage groups of four loci rhl1, rhp1, rhi1 and rhs1, which had previously been mapped on chromosomes 7H, 1H, 6H and 5H, respectively, were enriched with new markers that flank the genes at a distance of 0.16 cM to 4.6 cM. The chromosomal position of three new genes – two that are responsible for the development of short root hairs (rhs2 and rhs3) and the gene that controls an irregular root hair pattern (rhi2) – were mapped on chromosomes 6H, 2H and 1H, respectively. A comparative analysis of the agrobotanical parameters between some mutants and their respective parental lines showed that mutations in genes responsible for root hair development had no effect on the agrobotanical performance of plants that were grown under controlled conditions. The presented mutant collection is a valuable tool for further identification of genes controlling root hair development in barley. © The Author(s) 2014},
note = {14},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Root hairs are tubular outgrowths of specialized epidermal cells called trichoblasts. They affect anchoring plants in soil, the uptake of water and nutrients and are the sites of the interaction between plants and microorganisms. Nineteen root hair mutants of barley representing different stages of root hair development were subjected to detailed morphological and genetic analyses. Each mutant was monogenic and recessive. An allelism test revealed that nine loci were responsible for the mutated root hair phenotypes in the collection and 1–4 mutated allelic forms were identified at each locus. Genetic relationships between the genes responsible for different stages of root hair formation were established. The linkage groups of four loci rhl1, rhp1, rhi1 and rhs1, which had previously been mapped on chromosomes 7H, 1H, 6H and 5H, respectively, were enriched with new markers that flank the genes at a distance of 0.16 cM to 4.6 cM. The chromosomal position of three new genes – two that are responsible for the development of short root hairs (rhs2 and rhs3) and the gene that controls an irregular root hair pattern (rhi2) – were mapped on chromosomes 6H, 2H and 1H, respectively. A comparative analysis of the agrobotanical parameters between some mutants and their respective parental lines showed that mutations in genes responsible for root hair development had no effect on the agrobotanical performance of plants that were grown under controlled conditions. The presented mutant collection is a valuable tool for further identification of genes controlling root hair development in barley. © The Author(s) 2014
2001
Nawrot, M.; Szarejko, I.; Maluszynski, M.
Barley mutants with increased tolerance to aluminium toxicity Journal Article
In: Euphytica, vol. 120, no. 3, pp. 345-356, 2001, ISSN: 00142336, (19).
@article{2-s2.0-0034797393,
title = {Barley mutants with increased tolerance to aluminium toxicity},
author = { M. Nawrot and I. Szarejko and M. Maluszynski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034797393&doi=10.1023%2fA%3a1017565121835&partnerID=40&md5=a36911538f66d278ee9d755d17e8ca34},
doi = {10.1023/A:1017565121835},
issn = {00142336},
year = {2001},
date = {2001-01-01},
journal = {Euphytica},
volume = {120},
number = {3},
pages = {345-356},
abstract = {Acid soil and associated aluminium toxicity are considered as the number one abiotic factor limiting crop production. Over 2 billion hectares of acid soils exist world-wide, both in tropical and moderate climatic zones. In Poland acid soils represent up to 60% of arable land. At soil pH<5.0 Al ions become soluble in water and toxic to plants. Genetic improvement of Al tolerance in crops is the only alternative to soil liming, a traditional but short term and expensive agricultural cure to raise soil pH. Of the various cereals, barley is the most sensitive to Al toxicity. The known sources of Al tolerance in barley are limited to old cultivars and landraces. While they represent multiple alleles of a single locus, there is no potential to improve Al tolerance through recombination of non-allelic additive genes. In the Department of Genetics, Silesian University we have employed induced mutations for rapid creation of variability for Al tolerance in barley. Thirteen mutants with increased levels of tolerance to Al toxicity have been selected in M3 generation after mutagenic treatment of four barley varieties with N-methyl-N-nitroso urea (MNH) and sodium azide. Six further Al tolerant mutants were identified in the collection of semi-dwarf mutants of the Department. All selected mutants confirmed Al tolerance with the use of three different methods of screening, i.e., root re-growth, root tolerance index and hematoxylin staining. Fourteen mutants exhibited significant root re-growth after 48 hour incubation with 3 ppm Al+3 and two of them, namely RL819/2 and RL820/6 were tolerant even to 6 ppm Al+3. Crosses of two selected mutants with their respective parent varieties indicated that Al tolerance in each mutant was controlled by a single recessive gene. Out of three methods tested, the root re-growth method facilitated by hematoxylin staining proved to be the most reliable technique for large scale testing. Double treatment with MNH or combined treatment with sodium azide and MNH and 6h inter-incubation germination between treatments were the most successful treatment combinations for induction of aluminium tolerance in barley.},
note = {19},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Acid soil and associated aluminium toxicity are considered as the number one abiotic factor limiting crop production. Over 2 billion hectares of acid soils exist world-wide, both in tropical and moderate climatic zones. In Poland acid soils represent up to 60% of arable land. At soil pH<5.0 Al ions become soluble in water and toxic to plants. Genetic improvement of Al tolerance in crops is the only alternative to soil liming, a traditional but short term and expensive agricultural cure to raise soil pH. Of the various cereals, barley is the most sensitive to Al toxicity. The known sources of Al tolerance in barley are limited to old cultivars and landraces. While they represent multiple alleles of a single locus, there is no potential to improve Al tolerance through recombination of non-allelic additive genes. In the Department of Genetics, Silesian University we have employed induced mutations for rapid creation of variability for Al tolerance in barley. Thirteen mutants with increased levels of tolerance to Al toxicity have been selected in M3 generation after mutagenic treatment of four barley varieties with N-methyl-N-nitroso urea (MNH) and sodium azide. Six further Al tolerant mutants were identified in the collection of semi-dwarf mutants of the Department. All selected mutants confirmed Al tolerance with the use of three different methods of screening, i.e., root re-growth, root tolerance index and hematoxylin staining. Fourteen mutants exhibited significant root re-growth after 48 hour incubation with 3 ppm Al+3 and two of them, namely RL819/2 and RL820/6 were tolerant even to 6 ppm Al+3. Crosses of two selected mutants with their respective parent varieties indicated that Al tolerance in each mutant was controlled by a single recessive gene. Out of three methods tested, the root re-growth method facilitated by hematoxylin staining proved to be the most reliable technique for large scale testing. Double treatment with MNH or combined treatment with sodium azide and MNH and 6h inter-incubation germination between treatments were the most successful treatment combinations for induction of aluminium tolerance in barley.
1999
Gaj, M. D.; Czaja, G.; Nawrot, M.
Selection of valine-resistance in callus culture of Arabidopsis thaliana (L.) Heynh. Derived from leaf explants Journal Article
In: Acta Societatis Botanicorum Poloniae, vol. 68, no. 3, pp. 211-215, 1999, ISSN: 00016977, (1).
@article{2-s2.0-0033265539,
title = {Selection of valine-resistance in callus culture of Arabidopsis thaliana (L.) Heynh. Derived from leaf explants},
author = { M.D. Gaj and G. Czaja and M. Nawrot},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033265539&partnerID=40&md5=605a01483d2938d9cc057244f279742d},
issn = {00016977},
year = {1999},
date = {1999-01-01},
journal = {Acta Societatis Botanicorum Poloniae},
volume = {68},
number = {3},
pages = {211-215},
publisher = {Polish Botanical Society},
abstract = {The selection of valine-resistant mutants was carried out in leaf explant cultures of three Arabidopsis thaliana (L.) Heynh. ecotypes: C-24, RLD and Columbia. The valine concentration used for in vitro selection, lethal for seed-growing plants, has not affected callus formation and growth. However, strong inhibition of shoot regeneration ability of calli growing under selection pressure was noticed. In total, 1043 explants were cultured on valine medium and 18 shoots were regenerated with an average frequency of 1.7 shoots per 100 calli. Most R1 shoots were sterile and seeds were collected from 3 plants. The transmission of valine-resistance to the sexual progeny of these plants was scored and the increased level of valine-resistance was found in progeny of one line - 61C. This line originated from the culture of Columbia leaf explant and displayed tetraploid chromosome number.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The selection of valine-resistant mutants was carried out in leaf explant cultures of three Arabidopsis thaliana (L.) Heynh. ecotypes: C-24, RLD and Columbia. The valine concentration used for in vitro selection, lethal for seed-growing plants, has not affected callus formation and growth. However, strong inhibition of shoot regeneration ability of calli growing under selection pressure was noticed. In total, 1043 explants were cultured on valine medium and 18 shoots were regenerated with an average frequency of 1.7 shoots per 100 calli. Most R1 shoots were sterile and seeds were collected from 3 plants. The transmission of valine-resistance to the sexual progeny of these plants was scored and the increased level of valine-resistance was found in progeny of one line - 61C. This line originated from the culture of Columbia leaf explant and displayed tetraploid chromosome number.