@article{2-s2.0-85211618567,

title = {The paradoxical effects of beneficial bacteria on Solanum lycopersicum under Cd stress},

author = { M. Noszczyńska and M. Pawlik and M. Rudnicka and D. Wójcik and M. Gajecka and K. Kukucz and M. Skowronek and I.W. Potocka and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85211618567&doi=10.1016%2fj.envpol.2024.125430&partnerID=40&md5=4ce49372be49e0ed95cdb86bd4376fe1},

doi = {10.1016/j.envpol.2024.125430},

year  = {2025},

date = {2025-01-01},

journal = {Environmental Pollution},

volume = {366},

publisher = {Elsevier Ltd},

abstract = {This study investigated the complex interactions between a novel consortium and tomato seedlings under cadmium (Cd) stress. The consortium consists of two bacteria, Pseudomonas sp. HS4 and Paenarthrobacter sp. AS8, both with proven plant growth-promoting (PGP) properties, isolated from Cd hyperaccumulators. Our research highlights the paradoxical effects of these bacteria, revealing their dual role in reducing Cd uptake while simultaneously inducing oxidative stress in plants. Hydroponic experiments showed that the consortium reduced Cd accumulation in tomato shoots by 52% compared to uninoculated controls. However, this reduction was accompanied by decreased plant biomass and increased oxidative stress, with malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) levels up to 80% and 160% higher, respectively, in inoculated plants. Root H₂O₂ production increased by 38% under 50 μM Cd without a corresponding rise in catalase (CAT) activity. Despite Cd exposure, the consortium promoted chlorophyll and carotenoid synthesis, restoring pigment levels to those of unstressed controls. Gene expression analysis revealed a complex impact on stress responses, with inoculation suppressing Sl1 gene expression in roots and upregulating the oxidative stress-related GR-1 gene in shoots. These findings highlight the complex and multifaceted relationship between beneficial bacteria and plant fitness under heavy metal stress, with significant implications for sustainable agriculture. The study raises new questions regarding the broader physiological and ecological impacts of applying hyperaccumulator-associated bacteria in crop management, emphasizing the necessity for deeper mechanistic insights into these interactions to fully harness their potential in improving crop resilience and productivity. © 2024 Elsevier Ltd},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85184448307,

title = {Functional diversity of microbial communities in herbaceous vegetation patches in coal mine heaps},

author = { M. Malicka and W.M. Bierza and M. Szalbot and A. Kompała-Bąba and A. Błońska and F. Magurno and Z. Piotrowska-Seget and G. Woźniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184448307&doi=10.1002%2fldr.5055&partnerID=40&md5=514fdcf0817d751b97b60e09a6312d13},

doi = {10.1002/ldr.5055},

year  = {2024},

date = {2024-01-01},

journal = {Land Degradation and Development},

volume = {35},

number = {6},

pages = {2214-2225},

publisher = {John Wiley and Sons Ltd},

abstract = {Coal mine heaps represent unique novel environments, suitable for studying plant succession and its influence on the activity of microbes inhabiting the rhizosphere. Our aim was to verify if the functional diversity and catabolic activity of soil microorganisms would increase along with the plant succession from non-vegetated and forbs-dominated to grass-dominated communities. The study was conducted on coal mine heaps located in Upper Silesia (Southern Poland), focusing on non-vegetated patches, patches dominated by forbs–Tussilago farfara and Daucus carota (in the early stages of succession), and by grasses–Poa compressa and Calamagrostis epigejos (in later stages of primary succession). The catabolic activity and functional diversity of soil microbial communities were analyzed based on community-level physiological profiles using BIOLOG EcoPlatesTM and the activity of dehydrogenase, alkaline phosphatase, acid phosphatase, and urease. Our results showed that spontaneous vegetation on coal mine heaps strongly affects the physicochemistry of the substrate and the functional diversity of soil microbial communities. Grasses' rhizosphere was hosting more active and functional diversified microbial communities, while non-vegetated and T. farfara-vegetated patches were accompanied by a reduced development of soil microbiota. Furthermore, grasses were mainly associated with a substantial delivery of plant litter to the substrate, providing a source of carbon for microorganisms. © 2024 John Wiley & Sons Ltd.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85163682040,

title = {The microbial removal of bisphenols in aquatic microcosms and associated alteration in bacterial community},

author = { M. Noszczyńska and M. Pacwa-Płociniczak and K. Bondarczuk and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163682040&doi=10.1007%2fs11356-023-28305-2&partnerID=40&md5=db05224a4ae882bbfa3bf5f18d5f2125},

doi = {10.1007/s11356-023-28305-2},

issn = {09441344},

year  = {2023},

date = {2023-01-01},

journal = {Environmental Science and Pollution Research},

volume = {30},

number = {36},

pages = {85292-85304},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {The concept of the study resulted from numerous concerns around bisphenol A (BPA) and bisphenol S (BPS) in aquatic environments. In this study, river water and sediment microcosms highly polluted with bisphenols and bioaugmented with two BPs-removing bacterial strains were constructed. The study aimed to determine the rate of high-concentrated BPA and BPS (BPs) removal from river water and sediment microniches, and the effect of water bioaugmentation with bacterial consortium on the removal rates of these pollutants. Moreover, the impact of introduced strains and exposure to BPs on the structural and functional composition of the autochthonous bacterial communities was elucidated. Our findings indicate that the removal activity of autochthonous bacteria was sufficient for effectively BPA elimination and reducing BPS content in the microcosms. The number of introduced bacterial cells decreased continuously until day 40, and on consecutive sampling days, no bioaugmented cells were detected. Sequencing analysis of the total 16S rRNA genes revealed that the community composition in bioaugmented microcosms amended with BPs differed significantly from those treated either with bacteria or BPs. A metagenomic analysis found an increase in the abundance of proteins responsible for xenobiotics removal in BPs-amended microcosms. This study provides new insights into the effects of bioaugmentation with a bacterial consortium on bacterial diversity and BPs removal in aquatic environments. © 2023, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85156137329,

title = {The Effect of Plant Diversity and Soil Properties on Soil Microbial Biomass and Activity in a Novel Ecosystem},

author = { W.M. Bierza and G. Woźniak and A. Kompała-Bąba and F. Magurno and M. Malicka and D. Chmura and A. Błońska and A.M. Jagodziński and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85156137329&doi=10.3390%2fsu15064880&partnerID=40&md5=a9e44f6c8cd6ece6521cdc2e09110f47},

doi = {10.3390/su15064880},

issn = {20711050},

year  = {2023},

date = {2023-01-01},

journal = {Sustainability (Switzerland)},

volume = {15},

number = {6},

publisher = {MDPI},

abstract = {Plant–microbial relations have not yet been fully disclosed in natural or seminatural ecosys-tems, nor in novel ecosystems developing spontaneously on post-coal mine heaps. The aim of this study was to determine which factor, biotic (plant taxonomic diversity vs. plant functional diversity) or abiotic (physicochemical substrate parameters), affects the biomass of soil microbial communities the most, as well as soil in situ respiration in novel ecosystems. The study was carried out on unreclaimed plots selected according to four different combinations of taxonomic and functional plant diversity. Additionally, plots on a reclaimed heap served as a comparison between the two management types. The biomass of several soil microbial groups was analysed using phospholipid fatty acids profiles. We detected that soil microbial biomass was more impacted by abiotic parameters (explaining 23% of variance) than plant diversity (explaining 12% of variance). Particularly, we observed that substrate pH was the most important factor shaping microbial community biomass, as shown in the RDA analysis. The highest microbial biomass was found in plots with low taxonomic and functional diversity. This finding can be explained by the fact that these plots represented a more advanced phase of vegetation development in the early stages of plant succession. © 2023 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85149460033,

title = {Structure of the lipopolysaccharide O-antigen of endophytic Pseudomonas sp. strain L1},

author = { A. Choma and Z. Kaczyński and I. Komaniecka and A. Swatek and K. Kasperkiewicz and M. Pawlik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149460033&doi=10.1016%2fj.carres.2023.108779&partnerID=40&md5=1de182885720d739852b026656a7d36f},

doi = {10.1016/j.carres.2023.108779},

issn = {00086215},

year  = {2023},

date = {2023-01-01},

journal = {Carbohydrate Research},

volume = {525},

publisher = {Elsevier Ltd},

abstract = {The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Pseudomonas sp. Strain L1, the endophytic bacteria of Lolium perenne (ryegrass) plants growing in soil in an industrial area in the Silesia region (Zabrze; Southern Poland). The high-molecular-weight O-PS fraction liberated from Pseudomonas sp. L1 lipopolysaccharide by mild acid hydrolysis was studied using chemical methods, MALDI-TOF mass spectrometry, and 1D and 2D NMR spectroscopy techniques. It was found that the O-specific polysaccharide was built of tetrasaccharide repeating units composed of D-FucpN, D-Fucp4N, and two D-QuipN residues. The following structure of the O-PS of Pseudomonas sp. Strain L1 was established: [Formula presented] © 2023},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85141000751,

title = {Phenol and Polyaromatic Hydrocarbons Are Stronger Drivers Than Host Plant Species in Shaping the Arbuscular Mycorrhizal Fungal Component of the Mycorrhizosphere},

author = { M. Malicka and F. Magurno and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85141000751&doi=10.3390%2fijms232012585&partnerID=40&md5=19652d42bf272d5cc6138268a4af743f},

doi = {10.3390/ijms232012585},

issn = {16616596},

year  = {2022},

date = {2022-01-01},

journal = {International Journal of Molecular Sciences},

volume = {23},

number = {20},

publisher = {MDPI},

abstract = {Changes in soil microbial communities in response to hydrocarbon pollution are critical indicators of disturbed ecosystem conditions. A core component of these communities that is functionally adjusted to the life-history traits of the host and environmental factors consists of arbuscular mycorrhizal fungi (AMF). AMF communities associated with Poa trivialis and Phragmites australis growing at a phenol and polynuclear aromatic hydrocarbon (PAH)-contaminated site and at an uncontaminated site were compared based on LSU rDNA sequencing. Dissimilarities in species composition and community structures indicated soil pollution as the main factor negatively affecting the AMF diversity. The AMF communities at the contaminated site were dominated by fungal generalists (Rhizophagus; Funneliformis; Claroideoglomus; Paraglomus) with wide ecological tolerance. At the control site, the AMF communities were characterized by higher taxonomic and functional diversity than those exposed to the contamination. The host plant identity was the main driver distinguishing the two AMF metacommunities. The AMF communities at the uncontaminated site were represented by Polonospora, Paraglomus, Oehlia, Nanoglomus, Rhizoglomus, Dominikia, and Microdominikia. Polonosporaceae and Paraglomeraceae were particularly dominant in the Ph. australis mycorrhizosphere. The high abundance of early diverging AMF could be due to the use of primers able to detect lineages such as Paraglomeracae that have not been recognized by previously used 18S rDNA primers. © 2022 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85140470531,

title = {The Effect of Heavy Metals on Microbial Communities in Industrial Soil in the Area of Piekary Śląskie and Bukowno (Poland)},

author = { A. Jarosławiecka and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140470531&doi=10.3390%2fmicrobiolres13030045&partnerID=40&md5=bdfe7b2f20f5e3ab1e4c94aa669eee52},

doi = {10.3390/microbiolres13030045},

issn = {20367473},

year  = {2022},

date = {2022-01-01},

journal = {Microbiology Research},

volume = {13},

number = {3},

pages = {626-642},

publisher = {MDPI},

abstract = {The aim of this study was to determine the activity and structure of microbial communities in soils contaminated with heavy metals (HMs). To achieve this goal, soil samples were taken from two contaminated sites (i.e.; Piekary Śląskie and Bukowno) in Poland. A wide range of methods were applied, including: total and metal-tolerant culturable bacteria enumeration; microbial community structure analysis using the phospholipid fatty acid method (PLFA); denaturing gradient gel electrophoresis (PCR-DGGE); and metabolic activity using BIOLOG and EcoPlateTM. Our studies showed that HMs negatively affected microbial community structure and activity in polluted soils. Apart from the contamination with HMs, other soil parameters like soil pH and water also impacted microbial community structure and growth. Metal-tolerant bacterial strains were isolated, identified and tested for presence of genes encoding HM tolerance using the polymerase chain reaction (PCR) methodology. Contamination with HMs in the tested areas was found to lead to development of metallotolerant bacteria with multiple tolerances toward Zn, Ni, Cd and Cu. Different genes (e.g.; czcA; cadA and nccA) encoding HM efflux pumps were detected within isolated bacteria. Culturable bacteria isolated belonged to Proteobacteria, Actinobacteria and Bacteroidetes genera. Among non-culturable bacteria in soil samples, a significant fraction of the total bacteria and phyla, such as Gemmatimonadetes and Acidobacteria, were found to be present in all studied soils. In addition, bacteria of the Chloroflexi genus was present in soil samples from Piekary Śląskie, while bacteria of the Firmicutes genus were found in soil samples from Bukowno. © 2022 by the authors.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85129509887,

title = {Plant association with dark septate endophytes: When the going gets tough (and stressful), the tough fungi get going},

author = { M. Malicka and F. Magurno and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129509887&doi=10.1016%2fj.chemosphere.2022.134830&partnerID=40&md5=1f24aebe0a8254837975f7fc3f2c2432},

doi = {10.1016/j.chemosphere.2022.134830},

issn = {00456535},

year  = {2022},

date = {2022-01-01},

journal = {Chemosphere},

volume = {302},

publisher = {Elsevier Ltd},

abstract = {Dark septate endophytes (DSEs) comprise a diverse and ubiquitous group of fungal generalists with broad habitat niches that robustly colonize the roots of plants in stressful environments. DSEs possess adaptation strategies that determine their high tolerance to heavy metal (HM) contamination, drought, and salinity. Most DSEs developed efficient melanin-dependent and melanin-independent mechanisms of HM detoxification and osmoprotection, including intracellular immobilization and extracellular efflux of HMs and excess ions, and the scavenging of reactive oxygen species. DSEs form mutualistic relationship with plants according to the hypothesis of “habitat-adapted associations”, supporting the survival of their hosts under stressful conditions. As saprophytes, DSEs mineralize a complex soil substrate improving plants’ nutrition and physiological parameters. They can protect the host plant from HMs by limiting HM accumulation in plant tissues and causing their sequestration in root cell walls as insoluble compounds, preventing further HM translocation to shoots. The presence of DSE in drought-affected plants can substantially ameliorate the physiology and architecture of root systems, improving their hydraulic properties. Plant growth-promoting features, supported by the versatility and easy culturing of DSEs, determine their high potential to enhance phytoremediation and revegetation projects for HM-contaminated, saline, and desertic lands reclamation. © 2022 The Authors},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85125539700,

title = {High concentrations of HgS, MeHg and toxic gas emissions in thermally affected waste dumps from hard coal mining in Poland},

author = { Á. Nádudvari and J. Cabała and L. Marynowski and M. Jabłońska and M. Dziurowicz and D. Malczewski and B. Kozielska and P. Siupka and Z. Piotrowska-Seget and B.R.T. Simoneit and M. Szczyrba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125539700&doi=10.1016%2fj.jhazmat.2022.128542&partnerID=40&md5=dc4d622cf940b6c3ae23b6a2bebc9f65},

doi = {10.1016/j.jhazmat.2022.128542},

issn = {03043894},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Hazardous Materials},

volume = {431},

publisher = {Elsevier B.V.},

abstract = {This study aims to provide numerous environmental research approaches to understand the formation of mineral and organic mercury compounds in self-heating coal waste dumps of the Upper Silesian Coal Basin (USCB). The results are combined with environmental and health risk assessments. The mineralogy comprised accessory minerals in the fine fraction of thermally affected waste, i.e., Hg sulfides, most likely cinnabar or metacinnabar. Moreover, other metals, e.g., Pb, Zn and Cu, were found as sulfide forms. Apart from Hg, the ICP-ES/MS data confirmed the high content of Mn, Zn, Pb, Hg, Cr and Ba in these wastes. The high concentration of available Hg resulted in elevated MeHg concentrations in the dumps. There were no correlations or trends between MeHg concentrations and elemental Hg, TS, TOC, and pH. Furthermore, we did not detect microbial genes responsible for Hg methylation. The organic compounds identified in waste and emitted gases, such as organic acids, or free methyl radicals, common in such burn environments, could be responsible for the formation of MeHg. The concentration levels of gases, e.g., benzene, formaldehyde, NH3, emitted by the vents, reached or surpassed acceptable levels numerous times. The potential ecological and human health risks of these dumps were moderate to very high due to the significant influence of the high Hg concentrations. © 2022 Elsevier B.V.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85132818018,

title = {Outer Membrane Vesicles as Mediators of Plant–Bacterial Interactions},

author = { M. Rudnicka and M. Noszczyńska and M. Malicka and K. Kasperkiewicz and M. Pawlik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85132818018&doi=10.3389%2ffmicb.2022.902181&partnerID=40&md5=46f103a6c4d53db69a0da7d9312fbd41},

doi = {10.3389/fmicb.2022.902181},

issn = {1664302X},

year  = {2022},

date = {2022-01-01},

journal = {Frontiers in Microbiology},

volume = {13},

publisher = {Frontiers Media S.A.},

abstract = {Plants have co-evolved with diverse microorganisms that have developed different mechanisms of direct and indirect interactions with their host. Recently, greater attention has been paid to a direct “message” delivery pathway from bacteria to plants, mediated by the outer membrane vesicles (OMVs). OMVs produced by Gram-negative bacteria play significant roles in multiple interactions with other bacteria within the same community, the environment, and colonized hosts. The combined forces of innovative technologies and experience in the area of plant–bacterial interactions have put pressure on a detailed examination of the OMVs composition, the routes of their delivery to plant cells, and their significance in pathogenesis, protection, and plant growth promotion. This review synthesizes the available knowledge on OMVs in the context of possible mechanisms of interactions between OMVs, bacteria, and plant cells. OMVs are considered to be potential stimulators of the plant immune system, holding potential for application in plant bioprotection. Copyright © 2022 Rudnicka, Noszczyńska, Malicka, Kasperkiewicz, Pawlik and Piotrowska-Seget.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85109547295,

title = {Antifungal activity and biosynthetic potential of new streptomyces sp. MW-W600-10 strain isolated from coal mine water},

author = { P. Siupka and F.T. Hansen and A. Schier and S. Rocco and T. Sørensen and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109547295&doi=10.3390%2fijms22147441&partnerID=40&md5=13775f8fa8f0c93edaeabb59cf4538e2},

doi = {10.3390/ijms22147441},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {14},

publisher = {MDPI AG},

abstract = {Crop infections by fungi lead to severe losses in food production and pose risks for human health. The increasing resistance of pathogens to fungicides has led to the higher usage of these chemicals, which burdens the environment and highlights the need to find novel natural biocontrol agents. Members of the genus Streptomyces are known to produce a plethora of bioactive compounds. Recently, researchers have turned to extreme and previously unexplored niches in the search for new strains with antimicrobial activities. One such niche are underground coal mine environments. We isolated the new Streptomyces sp. MW-W600-10 strain from coal mine water samples collected at 665 m below ground level. We examined the antifungal activity of the strain against plant pathogens Fusarium culmorum DSM62188 and Nigrospora oryzae roseF7. Furthermore, we analyzed the strain’s biosynthetic potential with the antiSMASH tool. The strain showed inhibitory activity against both fungi strains. Genome mining revealed that it has 39 BGCs, among which 13 did not show similarity to those in databases. Additionally, we examined the activity of the Streptomyces sp. S-2 strain isolated from black soot against F. culmorum DSM62188. These results show that coal-related strains could be a source of novel bioactive compounds. Future studies will elucidate their full biotechnological potential. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85105830610,

title = {Differences in the effects of single and mixed species of AMF on the growth and oxidative stress defense in Lolium perenne exposed to hydrocarbons},

author = { M. Malicka and F. Magurno and K. Posta and D. Chmura and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105830610&doi=10.1016%2fj.ecoenv.2021.112252&partnerID=40&md5=293ad4644e65945d552f956a9ad8f128},

doi = {10.1016/j.ecoenv.2021.112252},

issn = {01476513},

year  = {2021},

date = {2021-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {217},

publisher = {Academic Press},

abstract = {Arbuscular mycorrhizal fungi (AMF) are ubiquitous mutualistic plant symbionts that promote plant growth and protect them from abiotic stresses. Studies on AMF-assisted phytoremediation have shown that AMF can increase plant tolerance to the presence of hydrocarbon contaminants by improving plant nutrition status and mitigating oxidative stress. This work aimed to evaluate the impact of single and mixed-species AMF inocula (Funneliformis caledonium; Diversispora varaderana; Claroideoglomus walkeri), obtained from a contaminated environment, on the growth, oxidative stress (DNA oxidation and lipid peroxidation), and activity of antioxidative enzymes (superoxide dismutase; catalase; peroxidase) in Lolium perenne growing on a substrate contaminated with 0/0–30/120 mg phenol/polynuclear aromatic hydrocarbons (PAHs) kg−1. The assessment of AMF tolerance to the presence of contaminants was based on mycorrhizal root colonization, spore production, the level of oxidative stress, and antioxidative activity in AMF spores. In contrast to the mixed-species AMF inoculum, single AMF species significantly enhanced the growth of host plants cultured on the contaminated substrate. The effect of inoculation on the level of oxidative stress and the activity of antioxidative enzymes in plant tissues differed between the AMF species. Changes in the level of oxidative stress and the activity of antioxidative enzymes in AMF spores in response to contamination also depended on AMF species. Although the concentration of phenol and PAHs had a negative effect on the production of AMF spores, low (5/20 mg phenol/PAHs kg−1) and medium (15/60 mg phenol/PAHs kg−1) substrate contamination stimulated the mycorrhizal colonization of roots. Among the studied AMF species, F. caledonium was the most tolerant to phenol and PAHs and showed the highest potential in plant growth promotion. The results presented in this study might contribute to the development of functionally customized AMF-assisted phytoremediation strategies with indigenous AMF, more effective than commercial AMF inocula, as a result of their selection by the presence of contaminants. © 2021 The Authors},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85096144505,

title = {A comprehensive study on bisphenol A degradation by newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12},

author = { M. Noszczyńska and M. Chodór and Ł. Jałowiecki and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096144505&doi=10.1007%2fs10532-020-09919-6&partnerID=40&md5=1dd7c00524c59806843995f2ec3955fc},

doi = {10.1007/s10532-020-09919-6},

issn = {09239820},

year  = {2021},

date = {2021-01-01},

journal = {Biodegradation},

volume = {32},

number = {1},

publisher = {Springer Science and Business Media B.V.},

abstract = {Bisphenol A (BPA) is an endocrine disrupting chemical. Its extensive use has led to the wide occurrence of BPA in various environmental ecosystems, at levels that may cause negative effects to the ecosystem and public health. Although there are many bacteria able to BPA utilization, only a few of them have a strong capacity for its biodegradation. Therefore, it is important to search for new bacteria strains, investigate their BPA biodegradation ability and potential effect of pH and other organic compounds on the process. These tasks have become the object of the present study. The results of our research show that for the newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12 after 15 days, with an initial BPA concentration of 100 mg L− 1, the highest BPA removal was achieved at pH 8, while sodium glutamate as a biostimulant best accelerated BPA degradation. Kinetic data for BPA biodegradation by both strains best fitted the Monod model. The specific degradation rate and the half saturation constant were estimated respectively as 8.75 mg L− 1 day− 1 and 111.27 mg L− 1 for Acinetobacter sp. K1MN, and 8.6 mg L− 1 day− 1 and 135.79 mg L− 1 for Pseudomonas sp. BG12. The half-maximal effective concentration (EC50) of BPA for Acinetobacter sp. K1MN was 120 mg L− 1 and for Pseudomonas sp. BG12 it was 123 mg L− 1. The toxicity bioassay (Microtox test) showed that elimination of BPA by both strains is accompanied by reduction of its toxic effect. The ability of tested strains to degrade BPA combined with their high resistance to this xenobiotic indicates that Acinetobacter sp. K1MN and Pseudomonas sp. BG12 are potential tools for BPA removal during wastewater treatment plant. © 2020, The Author(s).},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85096676877,

title = {Application of erythromycin and/or raoultella sp. Strain mc3 alters the metabolic activity of soil microbial communities as revealed by the community level physiological profiling approach},

author = { M. Cycoń and A. Markowicz and T.J. Wąsik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096676877&doi=10.3390%2fmicroorganisms8121860&partnerID=40&md5=ff253774567f12d907dba38a841165e6},

doi = {10.3390/microorganisms8121860},

issn = {20762607},

year  = {2020},

date = {2020-01-01},

journal = {Microorganisms},

volume = {8},

number = {12},

pages = {1-16},

publisher = {MDPI AG},

abstract = {Erythromycin (EM), a macrolide antibiotic, by influencing the biodiversity of microorganisms, might change the catabolic activity of the entire soil microbial community. Hence, the goal of this study was to determine the metabolic biodiversity in soil treated with EM (1 and 10 mg/kg soil) using the community-level physiological profiling (CLPP) method during a 90-day experiment. In addition, the effect of soil inoculation with antibiotic-resistant Raoultella sp. strain MC3 on CLPP was evaluated. The resistance and resilience concept as well as multifactorial analysis of data was exploited to interpret the outcomes obtained. EM negatively affected the metabolic microbial activity, as indicated by the values of the CLPP indices, i.e., microbial activity expressed as the average well-color development (AWCD), substrate richness (R), the Shannon–Wiener (H) and evenness (E) indices and the AWCD values for the six groups of carbon substrate present in EcoPlates until 15 days. The introduction of strain MC3 into soil increased the degradative activity of soil microorganisms in comparison with non-inoculated control. In contrast, at the consecutive sampling days, an increase in the values of the CLPP parameters was observed, especially for EM-10 + MC3-treated soil. Considering the average values of the resistance index for all of the measurement days, the resistance of the CLPP indices and the AWCD values for carbon substrate groups were categorized as follows: E > H > R > AWCD and polymers > amino acids > carbohydrates > miscellaneous > amines > carboxylic acids. The obtained results suggest a low level of resistance of soil microorganisms to EM and/or strain MC3 at the beginning of the exposure time, but the microbial community exhibited the ability to recover its initial decrease in catabolic activity over the experimental period. Despite the short-term effects, the balance of the soil ecosystem may be disturbed. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85090876104,

title = {Metagenomic Functional Profiling Reveals Differences in Bacterial Composition and Function During Bioaugmentation of Aged Petroleum-Contaminated Soil},

author = { M. Pacwa-Płociniczak and Pa. Biniecka and K. Bondarczuk and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090876104&doi=10.3389%2ffmicb.2020.02106&partnerID=40&md5=6be3628cada1c6c91e4f223debdaf477},

doi = {10.3389/fmicb.2020.02106},

issn = {1664302X},

year  = {2020},

date = {2020-01-01},

journal = {Frontiers in Microbiology},

volume = {11},

publisher = {Frontiers Media S.A.},

abstract = {Our objective was to study the bacterial community changes that determine enhanced removal of petroleum hydrocarbons from soils subjected to bioaugmentation with the hydrocarbon-degrading strains Rhodococcus erythropolis CD 130, CD 167, and their combination. To achieve this, a high-throughput sequencing of the 16S rRNA gene was performed. The changes in the bacterial community composition were most apparent the day after bacterial inoculation. These changes represented an increase in the percentage abundance of Rhodococcus and Pseudomonas genera. Surprisingly, members of the Rhodococcus genus were not present after day 91. At the end of the experiment, the bacterial communities from the CD 130, CD 167, and control soils had a similar structure. Nevertheless, the composition of the bacteria in the CD 130 + CD 167 soil was still distinct from the control. Metagenomic predictions from the 16S rRNA gene sequences showed that the introduction of bacteria had a significant influence on the predicted pathways (metabolism of xenobiotics; lipids; terpenoids; polyketides; and amino acids) on day one. On day 182, differences in the abundance of functional pathways were also detected in the CD 130 and CD 130 + CD 167 soils. Additionally, we observed that on day one, in all bioaugmented soils, the alkH gene was mainly contributed by the Rhodococcus and Mycobacterium genera, whereas in non-treated soil, this gene was contributed only by the Mycobacterium genus. Interestingly, from day 91, the Mycobacterium genus was the main contributor for the tested genes in all studied soils. Our results showed that hydrocarbon depletion from the analyzed soils resulted from the activity of the autochthonous bacteria. However, these changes in the composition and function of the indigenous bacterial community occurred under the influence of the introduced bacteria. © Copyright © 2020 Pacwa-Płociniczak, Biniecka, Bondarczuk and Piotrowska-Seget.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85090893280,

title = {Comparison of two inoculation methods of endophytic bacteria to enhance phytodegradation efficacy of an aged petroleum hydrocarbons polluted soil},

author = { M. Pawlik and T. Płociniczak and S. Thijs and I. Pintelon and J. Vangronsveld and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090893280&doi=10.3390%2fagronomy10081196&partnerID=40&md5=f2f787ff1be30dda008aa9d732e2907f},

doi = {10.3390/agronomy10081196},

issn = {20734395},

year  = {2020},

date = {2020-01-01},

journal = {Agronomy},

volume = {10},

number = {8},

publisher = {MDPI AG},

abstract = {Endophyte-enhanced phytodegradation is a promising technology to clean up polluted soils. To improve the success rate of this nature-based remediation approach, it is important to advance the inoculation method as this has been shown to strongly affect the final outcome. However, studies evaluating inoculation strategies and their effect on hydrocarbon degradation are limited. This study aims to investigate two different manners of endophyte inoculation in Lolium perenne growing in an aged petroleum hydrocarbon polluted soil: (1) direct soil inoculation (SI), and (2) pre-inoculation of the caryopses followed by soil inoculation (PI). Different endophytic bacterial strains, Rhodococcus erythropolis 5WK and Rhizobium sp. 10WK, were applied individually as well as in combination. Depending on the method of inoculation, the petroleum hydrocarbon (PHC) degradation potential was significantly different. The highest PHC removal was achieved after pre-inoculation of ryegrass caryopses with a consortium of both bacterial strains. Moreover, both strains established in the aged-polluted soil and could also colonize the roots and shoots of L. perenne. Importantly, used endophytes showed the selective colonization of the environment compartments. Our findings show that the method of inoculation determines the efficiency of the phytodegradation process, especially the rate of PHC degradation. This study provides valuable information for choosing the most cost-effective and beneficial means to optimize phytodegradation. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85089798964,

title = {A High manganese-tolerant pseudomonas sp. strain isolated from metallurgicalwaste heap can be a tool for enhancing manganese removal from contaminated soil},

author = { M. Noszczyńska and K. Łakomy and K. Nowacki and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089798964&doi=10.3390%2fapp10165717&partnerID=40&md5=fcced5b5bec5c0cdf10d6a679b4efe5c},

doi = {10.3390/app10165717},

issn = {20763417},

year  = {2020},

date = {2020-01-01},

journal = {Applied Sciences (Switzerland)},

volume = {10},

number = {16},

publisher = {MDPI AG},

abstract = {Manganese (Mn) is widely used in industry. However, its extensive applications have generated a great amount of manganese waste, which has become an ecological problem and has led to a decrease in natural resources. The use of microorganisms capable of accumulating Mn ions from contaminated ecosystems offers a potential alternative for the removal and recovery of this metal. The main aim of this work was an investigation of removal potential of Mn from soil by isolated bacterial. For this purpose, eleven bacterial strains were isolated from the soil from metallurgical waste heap in Upper Silesia, Poland. Strain named 2De with the highest Mn removal potential was selected and characterized taking into account its ability for Mn sorption and bioaccumulation from soil and medium containing manganese dioxide. Moreover, the protein profile of 2De strain before and after exposition to Mn was analyzed using SDS/PAGE technique. The 2De strain was identified as a Pseudomonas sp. The results revealed that this strain has an ability to grow at high Mn concentration and possesses an enhanced ability to remove it from the solution enriched with the soil or manganese dioxide via a biosorption mechanism. Moreover, changes in cellular protein expression of the isolated strain were observed. This study demonstrated that autochthonous 2De strain can be an effective tool to remove and recover Mn from contaminated soil. © 2020 by the authors.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85081964757,

title = {Microbial communities from subglacial water of naled ice bodies in the forefield of Werenskioldbreen, Svalbard},

author = { S. Sułowicz and K. Bondarczuk and D. Ignatiuk and J.A. Jania and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081964757&doi=10.1016%2fj.scitotenv.2020.138025&partnerID=40&md5=05556fab5373c731310fc10a0763a5d6},

doi = {10.1016/j.scitotenv.2020.138025},

issn = {00489697},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {723},

publisher = {Elsevier B.V.},

abstract = {We assessed the structure of microbial communities in the subglacial drainage system of the Werenskioldbreen glacier, Svalbard, which consists of three independent channels. Dome-shaped naled ice bodies that had been forming and releasing subglacial water in the glacial forefield during accumulations season were used to study glacial microbiome. We tested the hypothesis that the properties of the water transported by these channels are site-dependent and influence bacterial diversity. We therefore established the phylogenetic structure of the subglacial microbial communities using next generation sequencing (NGS) of the 16S rRNA gene and performed bioinformatics analyses. A total of 1409 OTUs (operational taxonomic units) belonged to 40 phyla; mostly Proteobacteria, Gracilibacteria, Bacteroidetes, Actinobacteria and Parcubacteria were identified. Sites located on the edge of Werenskioldbreen forefield (Angell; Kvisla) were mainly dominated by Betaproteobacteria. In the central site (Dusan) domination of Epsilonproteobacteria class was observed. Gracilibacteria (GN02) and Gammaproteobacteria represented the dominant taxa only in the sample Kvisla 2. Principal Coordinate Analysis (PCoA) of beta diversity revealed that phylogenetic profiles grouped in three different clusters according to the sampling site. Moreover, higher similarity of bacterial communities from Angell and Kvisla compared to Dusan was confirmed by cluster analysis and Venn diagrams. The highest alpha index values was measured in Dusan. Richness and phylogenetic diversity indices were significantly (p < .05) and positively correlated with pH values of subglacial water and negatively with concentration of Cl−, Br−, and NO3 − anions. These anions negatively impacted the values of richness indices but positively correlated with abundance of some microbial phyla. Our results indicated that subglacial water from naled ice bodies offer the possibility to study the glacial microbiome. In the studied subglacial water, the microbial community structure was sampling site specific and dependent on the water properties, which in turn were probably influenced by the local bedrock composition. © 2018},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85081025496,

title = {Response of rhizospheric and endophytic bacterial communities of white mustard (Sinapis alba) to bioaugmentation of soil with the Pseudomonas sp. H15 strain},

author = { T. Płociniczak and M. Pacwa-Płociniczak and M. Kwaśniewski and K. Chwiałkowska and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081025496&doi=10.1016%2fj.ecoenv.2020.110434&partnerID=40&md5=d8a193f0065138b2d4d3d54523d47078},

doi = {10.1016/j.ecoenv.2020.110434},

issn = {01476513},

year  = {2020},

date = {2020-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {194},

publisher = {Academic Press},

abstract = {A factor that may significantly increase the efficacy of phytoextraction is soil bioaugmentation with specific bacteria, which can alter the composition of rhizospheric and endophytic bacterial communities. The aim of this study was to compare the effect of soil treatment with living (bioaugmentation) and dead (control) cells of the plant growth-promoting metal-resistant endophytic strain Pseudomonas sp. H15 on the bacterial community composition in the rhizo- and endo-sphere of white mustard during enhanced phytoextraction. The bacterial communities in the rhizosphere were dominated (51.7–68.2%) by Proteobacteria, regardless of the soil treatment or sampling point. A temporary increase in the number of sequences belonging to Gammaproteobacteria (up to 37.3%) was only observed 24 h after the soil treatment with living Pseudomonas sp. H15 cells, whereas for the remaining samples, the relative abundance of this class did not exceed 7.1%. The relative abundance of Proteobacteria in the endosphere of the roots, stems, and leaves of white mustard was higher in the control than in bioaugmented plants. The most pronounced dominance of the Gammaproteobacteria sequences was observed in the stems and leaves of the control plants at the first sampling point, which strongly indicates the ability of the plants to rapidly uptake DNA from soil and translocate it to the aboveground parts of the plants. Additionally, the bioaugmentation of the soil caused a diverse shift in the bacterial communities in the rhizo- and endo-sphere of white mustard compared to control. The most distinct differences, which were dependent on the treatment, were observed in the endosphere of plants at the beginning of the experiment and decreased over time. These results indicate that the rhizo- and endo-biome of white mustard reacts to soil bioaugmentation and may influence the efficiency of bacterial-assisted phytoextraction. © 2020 Elsevier Inc.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85083227042,

title = {Genome mining revealed a high biosynthetic potential for antifungal streptomyces sp. S-2 isolated from black soot},

author = { P. Siupka and A. Piński and D. Babicka and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85083227042&doi=10.3390%2fijms21072558&partnerID=40&md5=8d03614905f0d09dac5423ffa3cca837},

doi = {10.3390/ijms21072558},

issn = {16616596},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Molecular Sciences},

volume = {21},

number = {7},

publisher = {MDPI AG},

abstract = {The increasing resistance of fungal pathogens has heightened the necessity of searching for new organisms and compounds to combat their spread. Streptomyces are bacteria that are well-known for the production of many antibiotics. To find novel antibiotic agents, researchers have turned to previously neglected and extreme environments. Here, we isolated a new strain, Streptomyces sp. S-2, for the first time, from black soot after hard coal combustion (collected from an in-use household chimney). We examined its antifungal properties against plant pathogens and against fungi that potentially pose threat to human health (Fusarium avenaceum; Aspergillus niger and the environmental isolates Trichoderma citrinoviridae Cin-9; Nigrospora oryzae sp. roseF7; and Curvularia coatesieae sp. junF9). Furthermore, we obtained the genome sequence of S-2 and examined its potential for secondary metabolites production using anti-SMASH software. The S-2 strain shows activity against all of the tested fungi. Genome mining elucidated a vast number of biosynthetic gene clusters (55), which distinguish this strain from closely related strains. The majority of the predicted clusters were assigned to non-ribosomal peptide synthetases or type 1 polyketide synthetases, groups known to produce compounds with antimicrobial activity. A high number of the gene clusters showed no, or low similarity to those in the database, raising the possibility that S-2 could be a producer of novel antibiotics. Future studies on Streptomyces sp. S-2 will elucidate its full biotechnological potential. © 2020 by the authors.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85079037401,

title = {Arbuscular mycorrhizal and microbial profiles of an aged phenol–polynuclear aromatic hydrocarbon-contaminated soil},

author = { M. Malicka and F. Magurno and Z. Piotrowska-Seget and D. Chmura},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079037401&doi=10.1016%2fj.ecoenv.2020.110299&partnerID=40&md5=2ccb36e5091ba5ffbfd34a3b3060fa2f},

doi = {10.1016/j.ecoenv.2020.110299},

issn = {01476513},

year  = {2020},

date = {2020-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {192},

publisher = {Academic Press},

abstract = {Arbuscular mycorrhizal fungi (AMF) are ubiquitous, obligatory plant symbionts that have a beneficial influence on plants in contaminated environments. This study focused on evaluating the biomass and biodiversity of the AMF and microbial communities associated with Poa trivialis and Phragmites australis plants sampled at an aged site contaminated with phenol and polynuclear aromatic hydrocarbons (PAHs) and an uncontaminated control site. We analyzed the soil phospholipid fatty acid profile to describe the general structure of microbial communities. PCR-denaturing gradient gel electrophoresis with primers targeting the 18S ribosomal RNA gene was used to characterize the biodiversity of the AMF communities and identify dominant AMF species associated with the host plants in the polluted and control environments. The root mycorrhizal colonization and AMF biomass in the soil were negatively affected by the presence of PAHs and phenol, with no significant differences between the studied plant species, whereas the biodiversity of the AMF communities were influenced by the soil contamination and plant species. Soil contamination was more detrimental to the biodiversity of AMF communities associated with Ph. australis, compared to P. trivialis. Both species favored the development of different AMF species, which might be related to the specific features of their different root systems and soil microbial communities. The contaminated site was dominated by AMF generalists like Funneliformis and Rhizophagus, whereas in the control site Dominikia, Archaeospora, Claroideoglomus, Glomus, and Diversispora were also detected. © 2020 Elsevier Inc.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85074128230,

title = {Impact of temperature on the physicochemical, structural and biological features of copper-silica nanocomposites},

author = { M. Dulski and K. Dudek and J. Podwórny and S. Sułowicz and Z. Piotrowska-Seget and K. Malarz and A. Mrozek-Wilczkiewicz and K. Wolnica and K. Matus and J. Peszke and A. Nowak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074128230&doi=10.1016%2fj.msec.2019.110274&partnerID=40&md5=4ca0bcc3c03e0a4eaa660bc1dbe57b03},

doi = {10.1016/j.msec.2019.110274},

issn = {09284931},

year  = {2020},

date = {2020-01-01},

journal = {Materials Science and Engineering C},

volume = {107},

publisher = {Elsevier Ltd},

abstract = {Classical wet chemical synthesis was used to fabricate a hybrid composite that contained copper nanoparticles (average size ∼1 nm), which were embedded into a silicon oxide carrier. The structural and chemical alternations in the copper-functionalized silica were investigated in systems that were sintered at 573 K, 873 K, 1173 K, and 1473 K. A general trend, which was associated with the transformation of metallic copper with a cubic structure into copper(II) oxide with a monoclinic structure in the heat-treated systems, was found. XPS and FTIR spectroscopies also revealed the presence of copper(I) oxide, which formed a shell around the CuO. SEM and TEM showed gradual densification of the hybrid system at ever higher sintering temperatures, which corresponded with the gradual copper agglomeration. A temperature of 873 K was determined to be the temperature at which amorphous silica was transformed into cristoballite and tridymite, as well as the formation of a bulk-like copper structure. In relation to the physicochemical and structural data, high antimicrobial features that had a relatively low toxicity effect on the normal human fibroblasts (NHDF) below 250 mg/L was found for the initial copper-silica composite and the samples that were sintered at 573 K. In turn, a significant decrease in the biological impact was observed in the samples that were sintered at temperatures above 573 K. As a result, the paper discusses the model of structural modifications in copper-silica nanocomposite concerning their biological impact that was developed. © 2019 Elsevier B.V.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85066952395,

title = {Physicochemical and structural features of heat treated silver-silica nanocomposite and their impact on biological properties},

author = { M. Dulski and J. Peszke and J. Włodarczyk and S. Sułowicz and Z. Piotrowska-Seget and K. Dudek and J. Podwórny and K. Malarz and A. Mrozek-Wilczkiewicz and M. Zubko and A. Nowak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066952395&doi=10.1016%2fj.msec.2019.109790&partnerID=40&md5=fff97a32db51f403cd8ec9a6905fae62},

doi = {10.1016/j.msec.2019.109790},

issn = {09284931},

year  = {2019},

date = {2019-01-01},

journal = {Materials Science and Engineering C},

volume = {103},

publisher = {Elsevier Ltd},

abstract = {In the last few decades, many nanostructures with varying properties and possible applications have been developed. These materials have been intended to work in various environmental temperature conditions. In this context, the main challenge has been to comprehend the impact of synergic interaction between individual elements included in non-annealed materials in relation to systems subjected to temperature impact. Another problem has corresponded to the impact of thermal modification on organisms such as bacteria and human cells. Such problems can be solved by the fabrication of a nanocomposite with mono-dispersed 8 nm silver (Ag0 or Ag+) embedded into a silica carrier, followed by the analysis of the impact of heat treatment under various temperature conditions on its physicochemical features. Therefore, methodical studies reported in this text have shown an increase of silver particle size up to 170 nm, a decrease of its concentration, as well as the formation of sub-nanometer Ag+ and/or Ag2+ clusters as the temperature rises to 1173 K. In turn, the structurally disordered silica carrier had been entirely transformed to cristobalite and tridymite only at 1473 K as well as partial reduction of Ag2+ to Ag+. Simultaneously, inhibition of growth of Gram-positive and Gram-negative bacteria, as well as an increase in cytotoxicity towards human cells was observed as the temperature rose. As a final point, for the first time, a “pseudo” phase diagram of the structural alterations in the Ag/SiO2 nanocomposite has been created, as well as a model of silver-silica transformation to biological systems has been developed. © 2019 Elsevier B.V.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85072842358,

title = {Toward the Development of an Innovative Implant: NiTi Alloy Functionalized by Multifunctional β-TCP+Ag/SiO2 Coatings},

author = { M. Dulski and K. Dudek and D. Chalon and J. Kubacki and S. Sułowicz and Z. Piotrowska-Seget and A. Mrozek-Wilczkiewicz and R. Gawecki and A. Nowak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072842358&doi=10.1021%2facsabm.8b00510&partnerID=40&md5=785458b6b09dd22d299d70bb052dd9ea},

doi = {10.1021/acsabm.8b00510},

issn = {25766422},

year  = {2019},

date = {2019-01-01},

journal = {ACS Applied Bio Materials},

volume = {2},

number = {3},

pages = {987-998},

publisher = {American Chemical Society},

abstract = {In recent years, one of the more important and costly problems of modern medicine is the need to replace or supplement organs in order to improve the quality of human life. In this field, promising solutions seem to have been implants which are based on NiTi alloys with shape memory effects. Unfortunately, this material is susceptible to the corrosion and release of toxic nickel to the human organism. Hence, its application as a long-term material is strongly limited. Therefore, this paper presents a new solution which should help to improve the functionality of the NiTi alloy and elongate its medical stability to use. The idea was focused on functionalization of the implant surface by a biocompatible, multifunctional coating without any impact on the features of the substrate, i.e., the martensitic transformation responsible for shape memory effects. For this purpose, we prepared a colloidal suspension, composed of β-TCP (particle size ∼450 nm) and the Ag/SiO2 nanocomposite which due to the electrophoretic deposition (EPD) led to the formation of structurally atypical calcium phosphosilicate coating. Those biomaterials formed a crack-free coating, adhering well to the NiTi surface when distributed over the entire surface, with low concentration of metallic and oxide silver (<3 at. %). At the same time, the coat-forming materials had resulted in the growth of a Gram-negative bacterial biofilm. Additionally, the additive of the silver-silica composite enhances cell proliferation, effectively a few times higher than commonly used coat-forming materials (e.g.; pure β-TCP). © 2019 American Chemical Society.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85058032943,

title = {Metal-tolerant endophytic bacteria associated with Silene vulgaris support the Cd and Zn phytoextraction in non-host plants},

author = { T. Płociniczak and M. Chodór and M. Pacwa-Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058032943&doi=10.1016%2fj.chemosphere.2018.12.018&partnerID=40&md5=22d7bcbc236a88cd13ff51a612ec9e27},

doi = {10.1016/j.chemosphere.2018.12.018},

issn = {00456535},

year  = {2019},

date = {2019-01-01},

journal = {Chemosphere},

volume = {219},

pages = {250-260},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to isolate and characterise metal-resistant endophytic bacteria from the tissues of Silene vulgaris collected within the vicinity of non-ferrous steelworks in Katowice, Upper Silesia, Southern Poland. Twenty-four strains of metal-resistant endophytic bacteria that belong to 15 genera were isolated from the stems and leaves of Silene vulgaris. Most of these strains showed multiple plant growth-promoting capabilities. The most promising strains, Proteus vulgaris H7, Pseudomonas sp. H15, and Pseudomonas helmanticensis H16, were used in a pot experiment, and their impact on the biomass of white mustard and Zn and Cd accumulation was examined. Soil inoculation with the tested strains resulted in a higher fresh biomass of shoots, which increased by 74.5% (Proteus vulgaris H7), 121.7% (Pseudomonas sp. H15), and 142.2% (P. helmanticensis H16) compared to the control plants. The highest phytoextraction enhancement was caused by P. helmanticensis H16, which increased Zn and Cd accumulation in the shoot tissues by 43.8% and 112.6%, respectively. All of the tested strains were detected in the soil at the last sampling points, but only Proteus vulgaris H7 and Pseudomonas sp. H15 were capable of temporary colonisation of the roots of white mustard. None of the inoculants were found in the stems and leaves of the plants during the experimental period. The plant growth-promoting features of the isolates combined with their resistance to heavy metals and high survival in soil after inoculation make these strains good candidates for the promotion of plant growth and increased phytoremediation efficiency. © 2018 Elsevier Ltd},

note = {37},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85057054295,

title = {The effect of bioaugmentation of petroleum-contaminated soil with Rhodococcus erythropolis strains on removal of petroleum from soil},

author = { M. Pacwa-Płociniczak and J. Czapla and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057054295&doi=10.1016%2fj.ecoenv.2018.11.081&partnerID=40&md5=216b4d448e42c30bd416107f744858a2},

doi = {10.1016/j.ecoenv.2018.11.081},

issn = {01476513},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {169},

pages = {615-622},

publisher = {Academic Press},

abstract = {The aim of the study was to assess the impact of inoculation of petroleum-contaminated soil with the hydrocarbon-degrading bacterial strains Rhodococcus erythropolis CD 130 and CD 167 or their consortium on the removal of hydrocarbons from the soil. Additionally, changes in the activity and structure of soil autochthonous bacterial communities were studied. At the end of the experiment, the fastest hydrocarbon removal was seen in the soil treated with the CD 167 strain (38.40%) and was statistically higher compared to the removal of total petroleum hydrocarbons (TPH) observed in soils inoculated with strain CD 130 (29.8%) or bacterial consortium CD 130 + CD 167 (29.72%). The rifampicin-resistant CD 130 and CD 167 strains, introduced as single strains or a consortium, survived in the soil for 42 days. The introduction of gram-positive strains of R. erythropolis primarily caused an increase in the biomass of branched phospholipid fatty acids (PLFAs), characteristic for gram-positive bacteria. Nevertheless, changes in the concentrations of gram-positive and gram-negative PLFA markers were periodic, and at the end of the experiment, significant changes were observed only in the case of the soil bioaugmented with the CD 167 strain. After the bioaugmentation, higher values of substrate-induced respiration (SIR) were observed in all the inoculated soils compared to the non-inoculated control. Nonetheless, after 91 days of incubation, a significant decrease in soil respiration was observed in the soil treated with single CD 130 or CD 167 strains or with their consortium. The number of transcripts of the CYP153 gene obtained on days 91 and 182 reflected the results of the hydrocarbon loss. The level of expression of the alkH gene in experimental soil was estimated and found to be higher than the level of expression of the CYP153 gene but did not coincide with the loss of hydrocarbons. The introduction of strains CD 130, CD 167, or CD 130 + CD 167 caused temporary changes in the composition of the soil autochthonous bacterial community, but it seems that these changes were needed for the enhanced removal of hydrocarbons from this soil. © 2018 Elsevier Inc.},

note = {33},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85054396540,

title = {Microbial diversity and antibiotic resistance in a final effluent-receiving lake},

author = { K. Bondarczuk and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054396540&doi=10.1016%2fj.scitotenv.2018.10.050&partnerID=40&md5=244cc16958de805d2f016af2384b4e68},

doi = {10.1016/j.scitotenv.2018.10.050},

issn = {00489697},

year  = {2019},

date = {2019-01-01},

journal = {Science of the Total Environment},

volume = {650},

pages = {2951-2961},

publisher = {Elsevier B.V.},

abstract = {Wastewater treatment plants have been recognised as hotspots for antibiotic resistance genes and antibiotic-resistant bacteria which enter the environment. However, the persistence of these genes and bacteria in receiving ecosystems remains poorly understood. The aim of the study was to evaluate the effect of final effluent release on microbial diversity and the antibiotic resistance gene pool in a final effluent-receiving lake. The numbers of total culturable heterotrophs and unculturable bacteria (represented as the 16S rRNA gene copy number) were significantly reduced during the treatment process. The number of ampicillin-resistant bacteria was higher in the sediment than in water samples, suggesting accumulation of ampicillin-resistant bacteria in freshwater sediments. Using an exogenous method, we captured 56 resistance plasmids which were further characterised. Next-generation sequencing revealed that the microbial phyla represented in the studied metagenomes were typical of corresponding environments. The highest relative abundance of antibiotic resistance genes was observed in the final effluent, suggesting that a considerable number of genes were released from the wastewater treatment plant. However, the lowest relative abundance and lowest diversity of the genes in the lake water, compared to the other studied metagenomes, suggest a negligible effect of treated sewage release on antibiotic resistance within water microbial communities of the lake. Furthermore, uncontrolled sewage dumping into this reservoir in the past as well as lower quality of the water upstream of the lake indicated that the wastewater treatment plant protected the studied ecosystem. © 2018 Elsevier B.V.},

note = {41},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85063987541,

title = {Antibiotics in the soil environment—degradation and their impact on microbial activity and diversity},

author = { M. Cycoń and A. Mrozik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063987541&doi=10.3389%2ffmicb.2019.00338&partnerID=40&md5=6c2e129b8c40a681605e54101c9850a5},

doi = {10.3389/fmicb.2019.00338},

issn = {1664302X},

year  = {2019},

date = {2019-01-01},

journal = {Frontiers in Microbiology},

volume = {10},

number = {MAR},

publisher = {Frontiers Media S.A.},

abstract = {Antibiotics play a key role in the management of infectious diseases in humans, animals, livestock, and aquacultures all over the world. The release of increasing amount of antibiotics into waters and soils creates a potential threat to all microorganisms in these environments. This review addresses issues related to the fate and degradation of antibiotics in soils and the impact of antibiotics on the structural, genetic and functional diversity of microbial communities. Due to the emergence of bacterial resistance to antibiotics, which is considered a worldwide public health problem, the abundance and diversity of antibiotic resistance genes (ARGs) in soils are also discussed. When antibiotic residues enter the soil, the main processes determining their persistence are sorption to organic particles and degradation/transformation. The wide range of DT50 values for antibiotic residues in soils shows that the processes governing persistence depend on a number of different factors, e.g., physicochemical properties of the residue, characteristics of the soil, and climatic factors (temperature; rainfall; and humidity). The results presented in this review show that antibiotics affect soil microorganisms by changing their enzyme activity and ability to metabolize different carbon sources, as well as by altering the overall microbial biomass and the relative abundance of different groups (i.e.; Gram-negative bacteria; Gram-positive bacteria; and fungi) in microbial communities. Studies using methods based on analyses of nucleic acids prove that antibiotics alter the biodiversity of microbial communities and the presence of many types of ARGs in soil are affected by agricultural and human activities. It is worth emphasizing that studies on ARGs in soil have resulted in the discovery of new genes and enzymes responsible for bacterial resistance to antibiotics. However, many ambiguous results indicate that precise estimation of the impact of antibiotics on the activity and diversity of soil microbial communities is a great challenge. Copyright © 2019 Cycoń, Mrozik and Piotrowska-Seget. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.},

note = {308},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85062723495,

title = {Glomalin gene as molecular marker for functional diversity of arbuscular mycorrhizal fungi in soil},

author = { F. Magurno and M. Malicka and K. Posta and G. Woźniak and E. Lumini and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062723495&doi=10.1007%2fs00374-019-01354-x&partnerID=40&md5=3e6b1c250d6b2f433e83e0599d5eda31},

doi = {10.1007/s00374-019-01354-x},

issn = {01782762},

year  = {2019},

date = {2019-01-01},

journal = {Biology and Fertility of Soils},

publisher = {Springer Verlag},

abstract = {Among the ecological services provided by arbuscular mycorrhizal fungi (AMF), the process of soil aggregation is hypothesized to be partially mediated by glomalin, an alkaline-soluble glycoprotein released by AM fungi into soil during hyphal turnover and after the death of the fungus in the soil. The protein is characterized by abundant production and hydrophobic properties. Although glomalin has been identified in Rhizophagus irregularis DAOM 197198 as a putative homolog of heat shock protein 60, the use of expressed fungal genes encoding glomalin as a marker for functional AMF diversity was never exploited. The present work describes the first attempt to identify the glomalin gene in several AMF species, verify its reliability as gene marker for the identification and discrimination of AMF, and test the possibility to detect its expression in soil. We designed a specific PCR primers set able to amplify many known lineages of AMF glomalin gene. We demonstrated its applicability to create a new reference glomalin sequence dataset for comparative sequence analysis. The designed primer set was successfully used to amplify glomalin transcript from soil cDNA template. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85054413580,

title = {Functional diversity of soil microbial communities in response to the application of cefuroxime and/or Antibiotic-resistant Pseudomonas putida strain MC1},

author = { K. Orlewska and A. Markowicz and Z. Piotrowska-Seget and J. Smoleń-Dzirba and M. Cycoń},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054413580&doi=10.3390%2fsu10103549&partnerID=40&md5=cc3a57be52885ff8a3c4b654eed24bc5},

doi = {10.3390/su10103549},

issn = {20711050},

year  = {2018},

date = {2018-01-01},

journal = {Sustainability (Switzerland)},

volume = {10},

number = {10},

publisher = {MDPI},

abstract = {Cefuroxime (XM), the most commonly prescribed antibiotic from the cephalosporin group, may cause changes in the structure of the soil microbial community, and these changes may also be reflected in the alteration of its functionality. Therefore, due to the lack of studies on this topic, the scope of this study was to assess the functional diversity and catabolic activity of the microbial community in soil treated with XM (1 mg/kg and 10 mg/kg soil) using the community-level physiological profile (CLPP) approach during a 90-day experiment. In addition, the effect of antibiotic-resistant Pseudomonas putida strain MC1 (Ps) was also evaluated. The resistance/resilience concept and multifactorial analysis were used to interpret the data. The results showed that the introduction of XM and/or Ps into the soil caused changes in the catabolic activity and functional diversity of the microbial community. A decrease in the values of the CLPP indices (i.e.; microbial activity expressed as the average well-color development (AWCD); substrate richness (R); the Shannon-Wiener (H) and evenness (E) indices and the AWCD values for the six carbon substrate groups) for the XM-treated soil was generally detected up to 30 days. In turn, at the same time, the activity measured in the Ps-inoculated soil was higher compared to the control soil. A stimulatory effect of XM at 10 mg/kg (XM10) and XM10+Ps on the utilization pattern of each substrate group was found at the following sampling times (days 60 and 90). The AWCD values for the utilization of amines, amino acids, carbohydrates, carboxylic acids, miscellaneous compounds and polymers for these treatments were found to be up to 2.3-, 3.1-, 2.3-, 13-, 3.4- and 3.3-fold higher compared to the values for the nontreated control, respectively. The resistance of the CLPP indices and the AWCD values for the carbon substrate groups were categorized as follows: E > H > R > AWCD and amino acids = carbohydrates > polymers > amines > miscellaneous > carboxylic acids, respectively. The results suggest a low initial resistance of the soil microbial community to XM and/or Ps, and despite the short-term negative effect, the balance of the soil ecosystem may be disturbed. © 2018 by the authors.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85050109911,

title = {Plant species and heavy metals affect biodiversity of microbial communities associated with metal-tolerant plants in metalliferous soils},

author = { S. Borymski and M. Cycoń and M. Beckmann and L.A.J. Mur and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050109911&doi=10.3389%2ffmicb.2018.01425&partnerID=40&md5=410b9d149c0eb79c9f95085bc3fd34e4},

doi = {10.3389/fmicb.2018.01425},

issn = {1664302X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Microbiology},

volume = {9},

number = {JUL},

publisher = {Frontiers Media S.A.},

abstract = {We here assess the biodiversity of the rhizosphere microbial communities of metal-tolerant plant species Arabidopsis arenosa, Arabidopsis halleri, Deschampsia caespitosa, and Silene vulgaris when growing on various heavy metal polluted sites. Our broad-spectrum analyses included counts for total and metal-tolerant culturable bacteria, assessments of microbial community structure by phospholipid fatty acid (PLFA) profiling and community-level analysis based on BIOLOG-CLPP to indicate functional diversity. The genetic-biochemical diversity was also measured by denaturing gradient gel electrophoresis (PCR-DGGE) and metabolomic analysis (HPLC-MS). Different rhizospheres showed distinctive profiles of microbial traits, which also differed significantly from bulk soil, indicating an influence from sampling site as well as plant species. However, total bacterial counts and PCR-DGGE profiles were most affected by the plants, whereas sampling site-connected variability was predominant for the PLFA profiles and an interaction of both factors for BIOLOG-CLPP. Correlations were also observed between pH, total and bioavailable Cd or Zn and measured microbial traits. Thus, both plant species and heavy-metals were shown to be major determinants of microbial community structure and function. © 2018 Borymski, Cycon, Beckmann, Mur and Piotrowska-Seget.},

note = {39},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85049081023,

title = {Use of the PCR-DGGE method for the analysis of the bacterial community structure in soil treated with the cephalosporin antibiotic cefuroxime and/or inoculated with a multidrug-resistant Pseudomonas putida strain MC1},

author = { K. Orlewska and Z. Piotrowska-Seget and M. Cycoń},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049081023&doi=10.3389%2ffmicb.2018.01387&partnerID=40&md5=f455b614ec7c962bb82d3e32404787ae},

doi = {10.3389/fmicb.2018.01387},

issn = {1664302X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Microbiology},

volume = {9},

number = {JUN},

publisher = {Frontiers Media S.A.},

abstract = {The widespread use of cefuroxime (XM) has resulted in the increase in its concentration in hospital and domestic wastewaters. Due to the limited removal of antibiotics and antibiotic-resistant genes in conventional systems, the drugs enter the surface water and soils. Moreover, the introduction of XM and/or XM-resistant bacteria into soil may cause a significant modification of the biodiversity of soil bacterial communities. Therefore, the goal of this research was to assess the genetic diversity of a bacterial community in the cefuroxime (XM1-1 mg/kg and XM10-10 mg/kg) and/or antibiotic-resistant Pseudomonas putida strain MC1 (Ps - 1.6 × 107 cells/g)-treated soils as determined by the DGGE (denaturing gradient gel electrophoresis) method. The obtained data were also evaluated using a multivariate analysis and the resistance (RS)/resilience (RL) concept. Strain MC1 was isolated from raw sewage in the presence of XM and was resistant not only to this antibiotic but also to vancomycin, clindamycin and erythromycin. The DGGE patterns revealed that the XM10 and XM10+Ps treatments modified the composition of the bacterial community by the alteration of the DGGE profiles as well as a decline in the DGGE indices, in particular on days 30, 60, and 90. In turn, the XM1 and XM1+Ps or Ps treatments did not affect the values of richness and diversity of the soil bacteria members. A principal component analysis (PCA) also indicated that XM markedly changed the diversity of bacterial assemblages in the second part of the experiment. Moreover, there were differences in the RS/RL of the DGGE indices to the disturbances caused by XM and/or Ps. Considering the mean values of the RS index, the resistance was categorized in the following order: diversity (0.997) > evenness (0.993) > richness (0.970). The soil RL index was found to be negative, thus reflecting the progressing detrimental impact of XM on the genetic biodiversity of bacteria within the experiment. These results indicate that the introduction of XM at higher dosages into the soil environment may exert a potential risk for functioning of microorganism. © 2018 Orlewska, Piotrowska-Seget and Cycon.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85042908378,

title = {Bisphenols: Application, occurrence, safety, and biodegradation mediated by bacterial communities in wastewater treatment plants and rivers},

author = { M. Noszczyńska and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042908378&doi=10.1016%2fj.chemosphere.2018.02.179&partnerID=40&md5=513698b7a546da494f121d268e6cdd83},

doi = {10.1016/j.chemosphere.2018.02.179},

issn = {00456535},

year  = {2018},

date = {2018-01-01},

journal = {Chemosphere},

volume = {201},

pages = {214-223},

publisher = {Elsevier Ltd},

abstract = {Numerous data indicate that most of bisphenols (BPs) are endocrine disrupters and exhibit cytotoxicity, neurotoxicity, genotoxicity and reproductive toxicity against vertebrates. Nevertheless, they are widely applied in material production what result in their ubiquitous occurrence in ecosystems. While BPA is the most frequently detected in environment, BPAF, BPF and BPS are also often found. Ecosystem particularly exposed to BPs pollution is industrial and municipal wastewater being a common source of BPA in river waters. Different techniques to remove BPs from these ecosystems have been applied, among which biodegradation seems to be the most effective. In this review the current state of knowledge in the field of BPs application, distribution in the environment, effects on animal and human health, and biodegradation mediated by bacterial populations in wastewater treatment plants and rivers is presented. © 2018 Elsevier Ltd},

note = {93},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85047296763,

title = {Vancomycin and/or multidrug-resistant Citrobacter Freundii altered the metabolic pattern of soil microbial community},

author = { M. Cycoń and K. Orlewska and A. Markowicz and A. Żmijowska and J. Smoleń-Dzirba and J. Bratosiewicz-Wąsik and T.J. Wąsik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047296763&doi=10.3389%2ffmicb.2018.01047&partnerID=40&md5=0db1de08bc9a069f674bf3dae722c3c2},

doi = {10.3389/fmicb.2018.01047},

issn = {1664302X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Microbiology},

volume = {9},

number = {MAY},

publisher = {Frontiers Media S.A.},

abstract = {Despite many studies, our knowledge on the impact of antibiotics and antibiotic-resistant bacteria on the metabolic activity of soil microbial communities is still limited. To ascertain this impact, the community level physiological profiles (CLPPs) and the activity of selected enzymes (dehydrogenase; urease; and phosphatases) in soils treated with vancomycin (VA) and/or multidrug resistant Citrobacter freundii were determined during a 90-day experiment. A multivariate analysis and the resistance (RS)/resilience (RL) concept were used to assess the potential of native microorganisms to maintain their catabolic activity under exposure of VA and/or a high level of C. freundii. In addition, the dissipation rate of VA was evaluated in non-sterile (nsS) and sterile (sS) soils. The results revealed a negative impact of VA on the metabolic activity of soil microorganisms on days 1, 15, and 30 as was showed by a decrease in the values of the CLPP indices (10-69%) and the enzyme activities (6-32%) for treated soils as compared to the control. These observations suggested a low initial resistance of soil microorganisms to VA and/or C. freundii but they were resilient in the long term. Considering the mean values of the RS index, the resistance of measured parameters was categorized in the following order: alkaline phosphatase (0.919) > acid phosphatase (0.899) > dehydrogenase (0.853) > the evenness index (0.840) > urease (0.833) > the Shannon-Wiener index (0.735) > substrate richness (0.485) > the AWCD (0.301). The dissipation process of VA was relatively fast and independent of the concentration used. The DT50 values for VA applied at both concentrations were about 16 days. In addition, the dissipation of VA in nsS was three times faster compared to the dissipation of antibiotic in sS. In conclusion, both CLPP and enzyme activities assays appeared to be useful tool for the determination of disturbances within soil microbial communities and used together may be helpful to understand the changes in their catabolic features. The entry of large quantities of VA and/or C. freundii into soil may temporarily change microbial activity thus pose a potential risk for soil functioning. © 2018 Cycon, Orlewska, Markowicz, Zmijowska, Smolen-Dzirba, Bratosiewicz-Wasik, Wasik and Piotrowska-Seget.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85042165581,

title = {Characterization of bacterial diversity in soil contaminated with the macrolide antibiotic erythromycin and/or inoculated with a multidrug-resistant Raoultella sp. strain using the PCR-DGGE approach},

author = { K. Orlewska and Z. Piotrowska-Seget and J. Bratosiewicz-Wąsik and M. Cycoń},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042165581&doi=10.1016%2fj.apsoil.2018.02.019&partnerID=40&md5=eed711f76f5b3608499eb6361e49b2f6},

doi = {10.1016/j.apsoil.2018.02.019},

issn = {09291393},

year  = {2018},

date = {2018-01-01},

journal = {Applied Soil Ecology},

volume = {126},

pages = {57-64},

publisher = {Elsevier B.V.},

abstract = {Antibiotics and microorganisms that are resistant to these drugs enter the soil via the use of sewage sludge, effluents and manure from antibiotic-treated animals and may cause significant changes in the diversity of microorganisms. The goal of this research was to evaluate the changes in the genetic diversity of a bacterial community in the soil after the addition of two different doses of erythromycin (EM) (1 and 10 mg/kg soil) and/or a multidrug-resistant Raoultella sp. strain (1.6 × 107 cells/g soil). The Raoultella sp. strain was isolated from raw sewage and expressed a resistance to erythromycin, clindamycin, tetracycline and vancomycin. A denaturing gradient gel electrophoresis (DGGE) analysis revealed that the pattern for the EM10-treated soil was significantly different from the one that was obtained for the control soil, which was also shown by a significant decrease in the values of the DGGE indices (diversity and richness) on days 15, 30 and 60. In turn, a lower dose of EM and/or the introduced bacterial strain had no effect on the diversity and richness of the dominant member of the soil bacterial community. A multivariate analysis demonstrated that the DGGE indices were significantly influenced by the dose of EM and the duration of the incubation. A principal component analysis (PCA) also revealed that EM and/or the bacterial strain significantly changed the microbial community structure on days 15, 30 and 60, which was also demonstrated by analyses of the PC scores using the MANOVA analysis for all of the sampling days and each day. Although the negative effect of a higher dose of EM on the biodiversity of soil bacteria (less number of genus) was transient, the application of EM into soil may temporarily pose a potential risk for soil functioning. © 2018 Elsevier B.V.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040236045,

title = {Effect of Silene vulgaris and Heavy Metal Pollution on Soil Microbial Diversity in Long-Term Contaminated Soil},

author = { M. Pacwa-Płociniczak and T. Płociniczak and D. Yu and J.M. Kurola and A. Sinkkonen and Z. Piotrowska-Seget and M. Romantschuk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040236045&doi=10.1007%2fs11270-017-3655-3&partnerID=40&md5=c3554399dc403e2252677ae08796fad4},

doi = {10.1007/s11270-017-3655-3},

issn = {00496979},

year  = {2018},

date = {2018-01-01},

journal = {Water, Air, and Soil Pollution},

volume = {229},

number = {1},

publisher = {Springer International Publishing},

abstract = {In this study, we analysed the impact of heavy metals and plant rhizodeposition on the structure of indigenous microbial communities in rhizosphere and bulk soil that had been exposed to heavy metals for more than 150 years. Samples of the rhizosphere of Silene vulgaris and non-rhizosphere soils 250 and 450 m from the source of emission that had different metal concentrations were collected for analyses. The results showed that soils were collected 250 m from the smelter had a higher number of Cd-resistant CFU compared with the samples that were collected from 450 m, but no significant differences were observed in the number of total and oligotrophic CFU or the equivalent cell numbers between rhizosphere and non-rhizosphere soils that were taken 250 and 450 m from the emitter. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis of the denaturing gradient gel electrophoresis (DGGE) profiles, as well as a cluster analysis that was generated on the phospholipid fatty acid (PLFA) profiles, showed that the bacterial community structure of rhizosphere soils depended more on the plant than on the distance and metal concentrations. The sequencing of the 16S rDNA fragments that were excised from the DGGE gel revealed representatives of the phyla Bacteroidetes, Acidobacteria, Gemmatimonadetes, Actinobacteria and Betaproteobacteria in the analysed soil with a predominance of the first three groups. The obtained results demonstrated that the presence of S. vulgaris did not affect the number of CFUs, except for those of Cd-resistant bacteria. However, the presence of S. vulgaris altered the soil bacterial community structure, regardless of the sampling site, which supported the thesis that plants have a higher impact on soil microbial community than metal contamination. © 2017, The Author(s).},

note = {34},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021779386,

title = {Hydrocarbon degradation potential and plant growth-promoting activity of culturable endophytic bacteria of Lotus corniculatus and Oenothera biennis from a long-term polluted site},

author = { M. Pawlik and B. Cania and S. Thijs and J. Vangronsveld and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021779386&doi=10.1007%2fs11356-017-9496-1&partnerID=40&md5=61028a54082d5dbe39342dba80f99a81},

doi = {10.1007/s11356-017-9496-1},

issn = {09441344},

year  = {2017},

date = {2017-01-01},

journal = {Environmental Science and Pollution Research},

volume = {24},

number = {24},

pages = {19640-19652},

publisher = {Springer Verlag},

abstract = {Many endophytic bacteria exert beneficial effects on their host, but still little is known about the bacteria associated with plants growing in areas heavily polluted by hydrocarbons. The aim of the study was characterization of culturable hydrocarbon-degrading endophytic bacteria associated with Lotus corniculatus L. and Oenothera biennis L. collected in long-term petroleum hydrocarbon-polluted site using culture-dependent and molecular approaches. A total of 26 hydrocarbon-degrading endophytes from these plants were isolated. Phylogenetic analyses classified the isolates into the phyla Proteobacteria and Actinobacteria. The majority of strains belonged to the genera Rhizobium, Pseudomonas, Stenotrophomonas, and Rhodococcus. More than 90% of the isolates could grow on medium with diesel oil, approximately 20% could use n-hexadecane as a sole carbon and energy source. PCR analysis revealed that 40% of the isolates possessed the P450 gene encoding for cytochrome P450-type alkane hydroxylase (CYP153). In in vitro tests, all endophytic strains demonstrated a wide range of plant growth-promoting traits such as production of indole-3-acetic acid, hydrogen cyanide, siderophores, and phosphate solubilization. More than 40% of the bacteria carried the gene encoding for the 1-aminocyclopropane-1-carboxylic acid deaminase (acdS). Our study shows that the diversity of endophytic bacterial communities in tested plants was different. The results revealed also that the investigated plants were colonized by endophytic bacteria possessing plant growth-promoting features and a clear potential to degrade hydrocarbons. The properties of isolated endophytes indicate that they have the high potential to improve phytoremediation of petroleum hydrocarbon-polluted soils. © 2017, The Author(s).},

note = {34},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85019267226,

title = {Improvement of phytoremediation of an aged petroleum hydrocarbon-contaminated soil by Rhodococcus erythropolis CD 106 strain},

author = { T. Płociniczak and E. Fic and M. Pacwa-Płociniczak and M. Pawlik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019267226&doi=10.1080%2f15226514.2016.1278420&partnerID=40&md5=2ccee0f9bdf71f304543948e2d5fd67f},

doi = {10.1080/15226514.2016.1278420},

issn = {15226514},

year  = {2017},

date = {2017-01-01},

journal = {International Journal of Phytoremediation},

volume = {19},

number = {7},

pages = {614-620},

publisher = {Taylor and Francis Inc.},

abstract = {The aim of this study was to assess the impact of soil inoculation with the Rhodococcus erythropolis CD 106 strain on the effectiveness of the phytoremediation of an aged hydrocarbon-contaminated [approx. 1% total petroleum hydrocarbon (TPH)] soil using ryegrass (Lolium perenne). The introduction of CD 106 into the soil significantly increased the biomass of ryegrass and the removal of hydrocarbons in planted soil. The fresh weight of the shoots and roots of plants inoculated with CD 106 increased by 49% and 30%, respectively. After 210 days of the experiment, the concentration of TPH was reduced by 31.2%, whereas in the planted, non-inoculated soil, it was reduced by 16.8%. By contrast, the concentration of petroleum hydrocarbon decreased by 18.7% in non-planted soil bioaugmented with the CD 106 strain. The rifampicin-resistant CD 106 strain survived after inoculation into soil and was detected in the soil during the entire experimental period, but the number of CD 106 cells decreased constantly during the enhanced phytoremediation and bioaugmentation experiments. The plant growth-promoting and hydrocarbon-degrading properties of CD 106, which are connected with its long-term survival and limited impact on autochthonous microflora, make this strain a good candidate for improving the phytoremediation efficiency of soil contaminated with hydrocarbons. © 2017 Taylor & Francis Group, LLC.},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021678961,

title = {Unique properties of silver and copper silica-based nanocomposites as antimicrobial agents},

author = { J. Peszke and M. Dulski and A. Nowak and K. Balin and M. Zubko and S. Sułowicz and B. Nowak and Z. Piotrowska-Seget and E. Talik and M. Wojtyniak and A. Mrozek-Wilczkiewicz and K. Malarz and J. Szade},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021678961&doi=10.1039%2fc7ra00720e&partnerID=40&md5=6c05b26f09aa8376a25c5d4cf449157d},

doi = {10.1039/c7ra00720e},

issn = {20462069},

year  = {2017},

date = {2017-01-01},

journal = {RSC Advances},

volume = {7},

number = {45},

pages = {28092-28104},

publisher = {Royal Society of Chemistry},

abstract = {The paper reports a new route for the fabrication and determination of physicochemical properties and biological activity, of metallic silica-based nanostructure (Ag/SiO2; Cu/SiO2). A research studies shows mono-dispersed nanoparticles in silica matrix with an average size of 12 nm for silver, as well as 12 nm and 4 nm, respectively for copper in hydrophobic and hydrophilic silica composites. The chemical analysis highlights metallic silver and copper ions heterogeneously distributed in the composite as well as metallic oxides such as Ag2O, Cu2O and CuO in hydrophobic system, and CuO in hydrophilic one. Structural research evidences the presence of amorphous, stoichiometric and non-stoichiometric crystalline phase of silica. Biological studies reveal potentially inhibition of growth gram-positive and gram-negative bacteria as well as microscopic fungi. The size of metal nanoparticles and level of silica hydrophobicity show the highest inhibition bacterial growth for hydrophilic system with embedding inside them, 4 nm in size copper nanoparticles. Finally, cytotoxic interaction against human cells with respect to silver and copper silica-based nanocomposites was not found. © 2017 The Royal Society of Chemistry.},

note = {32},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85007578640,

title = {Bioaugmentation as a strategy for the remediation of pesticide-polluted soil: A review},

author = { M. Cycoń and A. Mrozik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85007578640&doi=10.1016%2fj.chemosphere.2016.12.129&partnerID=40&md5=2937e4a9fbe5e1878ebf5f683b298531},

doi = {10.1016/j.chemosphere.2016.12.129},

issn = {00456535},

year  = {2017},

date = {2017-01-01},

journal = {Chemosphere},

volume = {172},

pages = {52-71},

publisher = {Elsevier Ltd},

abstract = {Bioaugmentation, a green technology, is defined as the improvement of the degradative capacity of contaminated areas by introducing specific microorganisms, has emerged as the most advantageous method for cleaning-up soil contaminated with pesticides. The present review discusses the selection of pesticide-utilising microorganisms from various sources, their potential for the degradation of pesticides from different chemical classes in liquid media as well as soil-related case studies in a laboratory, a greenhouse and field conditions. The paper is focused on the microbial degradation of the most common pesticides that have been used for many years such as organochlorinated and organophosphorus pesticides, triazines, pyrethroids, carbamate, chloroacetamide, benzimidazole and derivatives of phenoxyacetic acid. Special attention is paid to bacterial strains from the genera Alcaligenes, Arthrobacter, Bacillus, Brucella, Burkholderia, Catellibacterium, Pichia, Pseudomonas, Rhodococcus, Serratia, Sphingomonas, Stenotrophomonas, Streptomyces and Verticillum, which have potential applications in the bioremediation of pesticide-contaminated soils using bioaugmentation technology. Since many factors strongly influence the success of bioaugmentation, selected abiotic and biotic factors such as pH, temperature, type of soil, pesticide concentration, content of water and organic matter, additional carbon and nitrogen sources, inoculum size, interactions between the introduced strains and autochthonous microorganisms as well as the survival of inoculants were presented. © 2016 Elsevier Ltd},

note = {225},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85007072461,

title = {Activity and functional diversity of microbial communities in long-term hydrocarbon and heavy metal contaminated soils},

author = { A. Markowicz and G.A. Płaza and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85007072461&doi=10.1515%2faep-2016-0041&partnerID=40&md5=02b3df5c9b1d50a144d2330b194f62e2},

doi = {10.1515/aep-2016-0041},

issn = {03248461},

year  = {2016},

date = {2016-01-01},

journal = {Archives of Environmental Protection},

volume = {42},

number = {4},

pages = {3-11},

publisher = {De Gruyter Open Ltd},

abstract = {The impacts of long-term polycyclic aromatic hydrocarbons (PAHs) and heavy metal pollution on soil microbial communities functioning were studied in soils taken from an old coke plant. The concentrations of PAHs in the tested soils ranged from 171 to 2137 mg kg-1. From the group of tested heavy metals, concentrations of lead were found to be the highest, ranging from 57 to 3478 mg kg-1, while zinc concentrations varied from 247 to 704 mg kg-1 and nickel from 10 to 666 mg kg-1. High dehydrogenase, acid and alkaline phosphatase activities were observed in the most contaminated soil. This may indicate bacterial adaptation to long-term heavy metal and hydrocarbon contamination. However, the Community Level Physiological Profiles (CLPPs) analysis showed that the microbial functional diversity was reduced and influenced to a higher extent by some metals (Pb; Ni), moisture and conductivity than by PAHs. © 2016 Polish Academy of Sciences.},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84979735879,

title = {Prospects for arbuscular mycorrhizal fungi (AMF) to assist in phytoremediation of soil hydrocarbon contaminants},

author = { M. Malicka and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979735879&doi=10.1016%2fj.chemosphere.2016.07.071&partnerID=40&md5=df03e2ac4950cc5102c9911225e68928},

doi = {10.1016/j.chemosphere.2016.07.071},

issn = {00456535},

year  = {2016},

date = {2016-01-01},

journal = {Chemosphere},

volume = {162},

pages = {105-116},

publisher = {Elsevier Ltd},

abstract = {Arbuscular mycorrhizal fungi (AMF) form mutualistic associations with the roots of 80–90% of vascular plant species and may constitute up to 50% of the total soil microbial biomass. AMF have been considered to be a tool to enhance phytoremediation, as their mycelium create a widespread underground network that acts as a bridge between plant roots, soil and rhizosphere microorganisms. Abundant extramatrical hyphae extend the rhizosphere thus creating the hyphosphere, which significantly increases the area of a plant's access to nutrients and contaminants. The paper presents and evaluates the role and significance of AMF in phytoremediation of hydrocarbon contaminated sites. We focused on (1) an impact of hydrocarbons on arbuscular mycorrhizal symbiosis, (2) a potential of AMF to enhance phytoremediation, (3) determinants that influence effectiveness of hydrocarbon removal from contaminated soils. This knowledge may be useful for selection of proper plant and fungal symbionts and crucial to optimize environmental conditions for effective AMF-mediated phytoremediation. It has been concluded that three-component phytoremediation systems based on synergistic interactions between plant roots, AMF and hydrocarbon-degrading microorganisms demonstrated high effectiveness in dissipation of organic pollutants in soil. © 2016 Elsevier Ltd},

note = {58},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84975509465,

title = {Endophytic and rhizosphere bacteria associated with the roots of the halophyte Salicornia europaea L. - community structure and metabolic potential},

author = { S. Szymańska and T. Płociniczak and Z. Piotrowska-Seget and K. Hrynkiewicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975509465&doi=10.1016%2fj.micres.2016.05.012&partnerID=40&md5=c211d4eda85ae3e4d7080ee027477f32},

doi = {10.1016/j.micres.2016.05.012},

issn = {09445013},

year  = {2016},

date = {2016-01-01},

journal = {Microbiological Research},

volume = {192},

pages = {37-51},

publisher = {Elsevier GmbH},

abstract = {The main objective of our study was to assess density and diversity of rhizosphere (R) and endophytic (E) microorganisms associated with the halophyte S. europaea. Microorganisms were isolated from two saline sites (S1: 55 dS m-1; anthropogenic origin; S2: 112 dS m-1; natural salinity) located in central Poland and compared with microbial populations in the soil (S) using culture-independent (phospholipid fatty acids analysis; PLFA) and culture-dependent techniques. The endophytic and rhizosphere bacteria were identified and screened for nifH and acdS genes, and their metabolic properties were assessed. Strains with the potential to promote plant growth were selected for further study. PLFA analysis revealed that Gram-negative bacteria were dominant at both saline test sites; the total microbial biomass depended on the site (S1 < S2) and the zone of isolation (R > E > S). In contrast, culture-dependent techniques revealed that Gram-positive bacteria (Actinobacteria and Firmicutes) were dominant (S1: E-77.3%; R-86.3% and S2: E-59.1%; R-87.5%). Proteobacteria were observed in the rhizosphere at the lowest frequency (S1: 13.7% and S2: 12.5%). Greater salinity decreased the range and specificity of metabolic activity among the endophytes. These conditions also resulted in a broader spectrum of metabolic abilities in rhizobacteria; however, these metabolic processes were present at lower levels. © 2016 Elsevier GmbH.},

note = {42},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84993967987,

title = {Pyrethroid-degrading microorganisms and their potential for the bioremediation of contaminated soils: A review},

author = { M. Cycoń and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84993967987&doi=10.3389%2ffmicb.2016.01463&partnerID=40&md5=11a21c25798dfc06365e64213e289ab1},

doi = {10.3389/fmicb.2016.01463},

issn = {1664302X},

year  = {2016},

date = {2016-01-01},

journal = {Frontiers in Microbiology},

volume = {7},

number = {SEP},

publisher = {Frontiers Media S.A.},

abstract = {Pyrethroid insecticides have been used to control pests in agriculture, forestry, horticulture, public health and for indoor home use for more than 20 years. Because pyrethroids were considered to be a safer alternative to organophosphate pesticides (OPs), their applications significantly increased when the use of OPs was banned or limited. Although, pyrethroids have agricultural benefits, their widespread and continuous use is a major problem as they pollute the terrestrial and aquatic environments and affect non-target organisms. Since pyrethroids are not degraded immediately after application and because their residues are detected in soils, there is an urgent need to remediate pyrethroid-polluted environments. Various remediation technologies have been developed for this purpose; however, bioremediation, which involves bioaugmentation and/or biostimulation and is a cost-effective and eco-friendly approach, has emerged as the most advantageous method for cleaning-up pesticide-contaminated soils. This review presents an overview of the microorganisms that have been isolated from pyrethroid-polluted sites, characterized and applied for the degradation of pyrethroids in liquid and soil media. The paper is focused on the microbial degradation of the pyrethroids that have been most commonly used for many years such as allethrin, bifenthrin, cyfluthrin, cyhalothrin, cypermethrin, deltamethrin, fenpropathrin, fenvalerate, and permethrin. Special attention is given to the bacterial strains from the genera Achromobacter, Acidomonas, Bacillus, Brevibacterium, Catellibacterium, Clostridium, Lysinibacillus, Micrococcus, Ochrobactrum, Pseudomonas, Serratia, Sphingobium, Streptomyces, and the fungal strains from the genera Aspergillus, Candida, Cladosporium, and Trichoderma, which are characterized by their ability to degrade various pyrethroids. Moreover, the current knowledge on the degradation pathways of pyrethroids, the enzymes that are involved in the cleavage of pesticide molecules, the factors/conditions that influence the survival of strains that are introduced into soil and the rate of the removal of pyrethroids are also discussed. This knowledge may be useful to optimize the environmental conditions of bioremediation and may be crucial for the effective removal of pyrethroids from polluted soils. � 2016 Cycon and Piotrowska-Seget.},

note = {122},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84963591516,

title = {Monitoring the changes in a bacterial community in petroleum-polluted soil bioaugmented with hydrocarbon-degrading strains},

author = { M. Pacwa-Płociniczak and G.A. Płaza and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84963591516&doi=10.1016%2fj.apsoil.2016.04.005&partnerID=40&md5=08f6293adbad0098aea02d5034247bc4},

doi = {10.1016/j.apsoil.2016.04.005},

issn = {09291393},

year  = {2016},

date = {2016-01-01},

journal = {Applied Soil Ecology},

volume = {105},

pages = {76-85},

publisher = {Elsevier B.V.},

abstract = {Bioaugmentation is a strategy used to enhance degradation of petroleum compounds in contaminated soils, however little is known about the interactions between introduced bacteria and autochthonous microflora. Therefore the objective of the study was to assess any changes in the structure and metabolic activity of the soil bacterial communities as a result of the introduction of Bacillus subtilis T'-1 or Pseudomonas sp. P-1, as well their consortium, into petroleum-contaminated soil. The bioaugmentation experiment was carried out under laboratory conditions using soil taken from an industrial area located around a refinery in Czechowice-Dziedzice. After the bioaugmentation process, a significant (P < 0.05) decrease in the TPH content was reported in all inoculated soils. Inoculation of the soil with the bacterial consortium resulted in a three times greater removal of TPH compared to soils inoculated with single strains. It has been reported that all of the strains had an ability to survive in the environment during the experimental period. The introduction of bacterial strains led to increase in the number of 16S rRNA gene copies in soil on 1 and 7 days of the experiment as well as alkB gene copies during 91 days of the study compared to the non-treated soil. Analysis of the 16S rRNA and alkB genes-based DGGE fingerprints showed that introduced bacteria changed the genetic diversity of the total bacterial communities as well as the communities that have the genes involved in the degradation of hydrocarbons. Analysis of the PLFA profiles showed that the bacterial strains caused short-term changes in the amounts of fatty acids characteristic for Gram-positive and Gram-negative bacteria. The CLPPs indicated differences in soil metabolic activity between the inoculated and non-inoculated soils after the bioaugmentation process. © 2016 Elsevier B.V.},

note = {31},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84964290483,

title = {Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management},

author = { S. Sułowicz and M. Cycoń and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84964290483&doi=10.1007%2fs10646-016-1661-7&partnerID=40&md5=962c642379ac1442ccc8926e6cc36bb3},

doi = {10.1007/s10646-016-1661-7},

issn = {09639292},

year  = {2016},

date = {2016-01-01},

journal = {Ecotoxicology},

volume = {25},

number = {6},

pages = {1047-1060},

publisher = {Springer New York LLC},

abstract = {Effect of the fungicide tetraconazole on microbial community in silt loam soils from orchard with long history of triazole application and from grassland with no known history of fungicide usage was investigated. Triazole tetraconazole that had never been used on these soils before was applied at the field rate and at tenfold the FR. Response of microbial communities to tetraconazole was investigated during 28-day laboratory experiment by determination of changes in their biomass and structure (phospholipid fatty acids method—PLFA), activity (fluorescein diacetate hydrolysis—FDA) as well as changes in genetic (DGGE) and functional (Biolog) diversity. Obtained results indicated that the response of soil microorganisms to tetraconazole depended on the management of the soils. DGGE patterns revealed that both dosages of fungicide affected the structure of bacterial community and the impact on genetic diversity and richness was more prominent in orchard soil. Values of stress indices—the saturated/monounsaturated PLFAs ratio and the cyclo/monounsaturated precursors ratio, were almost twice as high and the Gram-negative/Gram-positive ratio was significantly lower in the orchard soil compared with the grassland soil. Results of principal component analysis of PLFA and Biolog profiles revealed significant impact of tetraconazole in orchard soil on day 28, whereas changes in these profiles obtained for grassland soil were insignificant or transient. Obtained results indicated that orchards soil seems to be more vulnerable to tetraconazole application compared to grassland soil. History of pesticide application and agricultural management should be taken into account in assessing of environmental impact of studied pesticides. © 2016, The Author(s).},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84976334412,

title = {Microbial community structure and diversity in long-term hydrocarbon and heavy metal contaminated soils},

author = { A. Markowicz and M. Cycoń and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84976334412&partnerID=40&md5=3b6357c3810e7048c0bbf0da6e7397a0},

issn = {17356865},

year  = {2016},

date = {2016-01-01},

journal = {International Journal of Environmental Research},

volume = {10},

number = {2},

pages = {321-323},

publisher = {University of Tehran},

abstract = {The influence of long-term mixed organic and inorganic contamination on soil microbial activity, community structure and genetic diversity was investigated in soil samples from a coke oven plant located in Upper Silesia, Poland. The tested soils were heavily contaminated with polycyclic aromatic hydrocarbons (PAHs) and heavy metals. The microbial communities were characterized using the phospholipid fatty acid (PLFA) and the denaturing gradient gel electrophoresis (DGGE) methods. Analysis of the PLFA profiles showed that the biomass of Gram-negative bacteria and fungi was affected by heavy metals but not by PAHs. Similar results were obtained for total microbial activity measured as the rate of fluorescein acetate hydrolysis. Statistical analysis of the obtained results revealed that heavy metals rather than PAHs were primarily responsible for the reduction in microbial activity and the differences in the microbial community structure as showed by PLFA. The DGGE analysis showed that the most contaminated soil had a very low biodiversity and richness but a very high evenness index. The correlation analysis revealed that the biodiversity and richness indices were negatively correlated with PAHs but not with heavy metals. However, there was a positive relationship between the evenness index and tested metals as well as the PAH content. The partial 16S rRNA sequence analysis showed that some of the clones were closely related to the genera Pseudomonas, Sphingomonas and Arthrobacter, which are well-known hydrocarbon degraders. Obtained results indicated that a high level of contamination suppress the some bacterial community member giving finally a reduction in the genetic diversity. © 2016, University of Tehran. All Rights Reserved.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84949845363,

title = {Isolation of hydrocarbon-degrading and biosurfactant-producing bacteria and assessment their plant growth-promoting traits},

author = { M. Pacwa-Płociniczak and T. Płociniczak and J. Iwan and M. Zarska and M. Chorazewski and M. Dzida and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949845363&doi=10.1016%2fj.jenvman.2015.11.058&partnerID=40&md5=a87f69d8c6d64170f34a59d83a5c2740},

doi = {10.1016/j.jenvman.2015.11.058},

issn = {03014797},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Environmental Management},

volume = {168},

pages = {175-184},

publisher = {Academic Press},

abstract = {Forty-two hydrocarbon-degrading bacterial strains were isolated from the soil heavily contaminated with petroleum hydrocarbons. Forty-one strains were identified based on their whole-cell fatty acid profiles using the MIDI-MIS method. Thirty-three of them belong to species Rhodococcus erythropolis, while the others to the genera Rahnella (4), Serratia (3) and Proteus (1). Isolates were screened for their ability to produce biosurfactants/bioemulsifiers. For all of them the activity of several mechanisms characteristic for plant growth-promoting bacteria was also determined. In order to investigate surface active and emulsifying abilities of isolates following methods: oil-spreading, blood agar, methylene blue agar and determination of emulsification index, were used. Among studied bacteria 12 strains (CD 112; CD 126; CD 131; CD 132; CD 135; CD 147; CD 154; CD 155; CD 158; CD 161; CD 166 and CD 167) have been chosen as promising candidates for the production of biosurfactants and/or bioemulsifiers. Among them 2 strains (R. erythropolis CD 126 and Rahnella aquatilis CD 132) had the highest potential to be used in the bioaugmentation of PH-contaminated soil. Moreover, 15 of tested strains (CD 105; CD 106; CD 108; CD 111; CD 116; CD 120; CD 124; CD 125; CD 130; CD 132; CD 134; CD 154; CD 156; CD 161 and CD 170) showed the activity of four mechanisms (ACC deaminase activity; IAA and siderophore production; phosphate solubilization) considered to be characteristic for plant growth-promoting bacteria. Two of them (R. erythropolis CD 106 and R. erythropolis CD 111) showed the highest activity of above-mentioned mechanisms and thus are considered as promising agents in microbe assisted phytoremediation. © 2015 Elsevier Ltd.},

note = {44},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84960129136,

title = {Rhizospheric bacterial strain brevibacterium casei MH8a colonizes plant tissues and enhances Cd, Zn, Cu phytoextraction by white mustard},

author = { T. Płociniczak and A. Sinkkonen and M. Romantschuk and S. Sułowicz and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960129136&doi=10.3389%2ffpls.2016.00101&partnerID=40&md5=e5f857a1ee07a62caa63d2653e3266aa},

doi = {10.3389/fpls.2016.00101},

issn = {1664462X},

year  = {2016},

date = {2016-01-01},

journal = {Frontiers in Plant Science},

volume = {7},

number = {FEB2016},

publisher = {Frontiers Media S.A.},

abstract = {Environmental pollution by heavy metals has become a serious problem in the world. Phytoextraction, which is one of the plant-based technologies, has attracted the most attention for the bioremediation of soils polluted with these contaminants. The aim of this study was to determine whether the multiple-tolerant bacterium, Brevibacterium casei MH8a isolated from the heavy metal-contaminated rhizosphere soil of Sinapis alba L., is able to promote plant growth and enhance Cd, Zn, and Cu uptake by white mustard under laboratory conditions. Additionally, the ability of the rifampicin-resistant spontaneous mutant of MH8a to colonize plant tissues and its mechanisms of plant growth promotion were also examined. In order to assess the ecological consequences of bioaugmentation on autochthonous bacteria, the phospholipid fatty acid (PLFA) analysis was used. The MH8a strain exhibited the ability to produce ammonia, 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid and HCN but was not able to solubilize inorganic phosphate and produce siderophores. Introduction of MH8a into soil sigNificantly increaSed S. alba biomass and the accumulation of Cd (208%), Zn (86%), and Cu (39%) in plant shoots in comparison with those grown in non-inoculated soil. Introduced into the soil, MH8a was able to enter the plant and was found in the roots and leaves of inoculated plants thus indicating its endophytic features. PLFA analysis revealed that the MH8a that was introduced into soil had a temporary influence on the structure of the autochthonous bacterial communities. The plant growth-promoting features of the MH8a strain and its ability to enhance the metal uptake by white mustard and its long-term survival in soil as well as its temporary impact on autochthonous microorganisms make the strain a suitable candidate for the promotion of plant growth and the efficiency of phytoextraction. © 2016, Płociniczak, Sinkkonen, Romantschuk, Sułowicz and Piotrowska-Seget.},

note = {38},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84961969270,

title = {The urgent need for risk assessment on the antibiotic resistance spread via sewage sludge land application},

author = { K. Bondarczuk and A. Markowicz and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961969270&doi=10.1016%2fj.envint.2015.11.011&partnerID=40&md5=3bd51b4e196ea839327eb4ed82636d9d},

doi = {10.1016/j.envint.2015.11.011},

issn = {01604120},

year  = {2016},

date = {2016-01-01},

journal = {Environment International},

volume = {87},

pages = {49-55},

publisher = {Elsevier Ltd},

abstract = {Sewage sludge is an ever-increasing by-product of the wastewater treatment process frequently used as a soil fertiliser. To control its quality and prevent any possible hazardous impact of fertilisation, some mandatory limits of heavy metal content have been established by the European Commission (Sewage Sludge Directive). However, since the implementation of the limits, new emerging contaminants have been reported worldwide. Regardless of the wastewater treatment process, sewage sludge contains antibiotics, antibiotic-resistant bacteria and antibiotic resistance genes, which can be released into the environment through its land application. Such a practice may even boost the dissemination and further development of antibiotic resistance phenomenon - already a global problem challenging modern medicine. Due to the growing pharmaceutical pollution in the environment, the time is ripe to assess the risk for the human and environmental health of sewage sludge land application in the context of antibiotic resistance spread. In this review we present the current knowledge in the field and we emphasise the necessity for more studies. © 2015 Elsevier Ltd.},

note = {175},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84945561417,

title = {Response of microbial communities from an apple orchard and grassland soils to the first-time application of the fungicide tetraconazole},

author = { S. Sułowicz and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84945561417&doi=10.1016%2fj.ecoenv.2015.10.025&partnerID=40&md5=b5a8e7f8783fa4973fcccfa6bd7990b0},

doi = {10.1016/j.ecoenv.2015.10.025},

issn = {01476513},

year  = {2016},

date = {2016-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {124},

pages = {193-201},

publisher = {Academic Press},

abstract = {The aim of the study was to assess the impact of the triazole fungicide tetraconazole applied at the field rate (FR) and at ten-fold the FR (10FR) on microorganisms in orchard soil with a long-term history of fungicides application and in grassland soil that had not previously been treated with pesticides. To ascertain this impact, the microbial activity determined by fluorescein diacetate (FDA) hydrolysis, the culturable number of bacteria, fungi and tetraconazole-resistant fungi, and the phospholipid microbial biomass and the structural and functional biodiversity assessed by the PLFA and Biolog approaches, respectively, were examined under laboratory conditions during 28-day experiment. The response of soil microorganisms to the fungicide tetraconazole, which had never been used before in these soils, depended on the management of the soils. In apple orchard soil that had been treated with FR or 10FR tetraconazole, a decrease in microbial activity was still observed on the 28th day after the application of the fungicide. In contrast, a significant impact of tetraconazole on the number of bacteria was still observed at the end of experiment in grassland soil. Results of principal component analysis (PCA) indicated that the application of tetraconazole significantly changed the structure of the microbial communities in the orchard soil. In addition, analysis of the Biolog profiles revealed a decrease in the catabolic activity of the microbial communities in grassland soil that had been treated with tetraconazole at both rates over time. The evaluation of the structural and functional diversity of microbial communities using PCA appears to be the most valuable monitoring tool for assessing the impact of tetraconazole application on soil microorganisms. © 2015 Elsevier Inc.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85008975728,

title = {Variable effects of non-steroidal anti-inflammatory drugs (NSAIDs) on selected biochemical processes mediated by soil microorganisms},

author = { M. Cycoń and S. Borymski and B. Zolnierczyk and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008975728&doi=10.3389%2ffmicb.2016.01969&partnerID=40&md5=2ef801e4453382647b6ddc916bcb945b},

doi = {10.3389/fmicb.2016.01969},

issn = {1664302X},

year  = {2016},

date = {2016-01-01},

journal = {Frontiers in Microbiology},

volume = {7},

number = {DEC},

publisher = {Frontiers Research Foundation},

abstract = {Non-steroidal anti-inflammatory drugs (NSAIDs) are the most frequently used group of pharmaceuticals. The high consumption and the uncontrolled disposal of unused drugs into municipal waste or their deposit in landfills can result in an increased concentration of these compounds in soils. Moreover, these drugs can affect the microbial activity. However, there is a lack of knowledge about these effects or it is very limited. Therefore, the objective of this study was to compare the impact of selected commercially available NSAIDs, i.e., diclofenac (DCF), naproxen (NPX), ibuprofen (IBF) and ketoprofen (KTP), applied at concentrations of 1 and 10 mg/kg soil, on the activity of soil microorganisms during the 90-day experiment. To ascertain this impact, substrate-induced respiration (SIR), soil enzyme activities, i.e., dehydrogenase (DHA), acid and alkaline phosphatases (PHOS-H and PHOS-OH) and urease (URE) as well as changes in the rates of nitrification and ammonification processes were determined. In addition, the number of culturable bacteria and fungi were enumerated. In general, the obtained data showed a significant stimulatory effect of NSAIDs on the microbial activity. Higher concentrations of NSAIDs caused a greater effect, which was observed for SIR, PHOS-H, PHOS-OH, URE, N-NO3- and N-NH4+, even during the whole incubation period. Moreover, the number of heterotrophic bacteria and fungi increased significantly during the experiment, which was probably a consequence of the evolution of specific microorganisms that were capable of degrading NSAIDs and used them as an additional source of carbon and energy. However, an inhibitory effect of NPX, IBF or KTP for SIR, DHA, on both phosphatases and culturable bacteria and fungi was observed at the beginning of the experiment. At lower concentrations of NSAIDs, in turn, the effects were negligible or transient. In conclusion, the application of NSAIDs altered the biochemical and microbial activity of soil what may cause the disturbance in soil functioning. It is reasonable to assume that some components of the NSAID formulations could stimulate soil microorganisms, thus resulting in an increase in biochemical activities of the soil. © 2016 Cycon, Borymski, Zolnierczyk and Piotrowska-Seget.},

note = {34},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84980384753,

title = {An analysis of the effects of vancomycin and/or vancomycin-resistant citrobacter freundii exposure on the microbial community structure in soil},

author = { M. Cycoń and S. Borymski and K. Orlewska and T.J. Wąsik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84980384753&doi=10.3389%2ffmicb.2016.01015&partnerID=40&md5=7721dc2e82ea50a36bac725b49913c32},

doi = {10.3389/fmicb.2016.01015},

issn = {1664302X},

year  = {2016},

date = {2016-01-01},

journal = {Frontiers in Microbiology},

volume = {7},

number = {JUN},

publisher = {Frontiers Research Foundation},

abstract = {The occurrence of antibiotics and antibiotic resistance genes in the environment has become a subject of growing concern. The extensive use of vancomycin and other pharmaceuticals may alter the biodiversity of soil microbial communities and select antibiotic-resistant bacteria. Therefore, the purpose of the study was to evaluate the impact of vancomycin and/or vancomycin-resistant Citrobacter freundii on soil microbial communities using the denaturing gradient gel electrophoresis (DGGE) and the phospholipid fatty acid (PLFA) approaches. The experiment had a completely randomized block design with the following treatments: Control soil (C), soil with vancomycin (1 mg/kg soil-VA1), soil with vancomycin (10 mg/kg soil-VA10), soil with C. freundii (Cit), soil with vancomycin (1 mg/kg soil) and C. freundii (VA1+Cit), and soil with vancomycin (10 mg/kg soil) and C. freundii (VA10+Cit). A bacterial strain resistant to vancomycin was isolated from raw sewage collected from the municipal sewage treatment plant. The obtained results indicated that the antibiotic and/or the bacterial strain exerted a selective pressure that resulted in qualitative and quantitative changes in the population of soil microorganisms. However, a multivariate analysis showed that the genetic and structural diversity of the soil microbial community was primarily affected by the incubation time and to a lesser extent by the antibiotic and introduced bacteria. DGGE analysis clearly showed that certain species within the bacterial community were sensitive to vancomycin as was evidenced by a decrease in the values of S (richness) and H (Shannon-Wiener) indices. Moreover, a PLFA method-based analysis revealed alterations in the structure of the soil microbial community as indicated by changes in the biomass of the PLFA biomarkers specific for Gram-positive and Gram-negative bacteria as well as fungi. The changes observed in the community of soil microorganisms may decrease the rate of microbial-mediated processes, which can lead to a disturbance in the ecological balance of the soil ecosystem. © 2016 Cycon, Borymski, Orlewska, Wasik and Piotrowska-Seget.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84963818721,

title = {The impact of fungicides on soil microorganisms [Oddziaływanie fungicydów na mikroorganizmy w srodowisku glebowym]},

author = { S. Sułowicz and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84963818721&partnerID=40&md5=bce6f5daef61d50179a21ff30d52296a},

issn = {00794252},

year  = {2016},

date = {2016-01-01},

journal = {Postepy Mikrobiologii},

volume = {55},

number = {1},

pages = {12-18},

publisher = {Polish Society of Microbiologists},

abstract = {Modern agriculture depends heavily on pesticides, including fungicides. Fungicides such as triazoles, when applied every year, may accumulate in soils leading to the development of resistance to the applied compounds and subsequently to the spread of resistance genes to other fungi. Additionally, fungicides can impact non-target soil microorganisms by reducing their biomass, changing microbial activity, and altering functional and structural diversity of bacterial and fungal communities. Soil quality is closely linked to the microbial activity, therefore, the effects of fungicides on non-target soil microorganisms increase concerns about the fertility of soil. This new knowledge about specific interaction between fungicides and soil microorganisms has to be taken into consideration in designing a new strategy for soil protection.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84949965050,

title = {Metabolic potential and community structure of endophytic and rhizosphere bacteria associated with the roots of the halophyte Aster tripolium L.},

author = { S. Szymańska and T. Płociniczak and Z. Piotrowska-Seget and M. Złoch and S. Ruppel and K. Hrynkiewicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949965050&doi=10.1016%2fj.micres.2015.09.007&partnerID=40&md5=76e362b94f74e4bc57cf0d1b286542e2},

doi = {10.1016/j.micres.2015.09.007},

issn = {09445013},

year  = {2016},

date = {2016-01-01},

journal = {Microbiological Research},

volume = {182},

pages = {68-79},

publisher = {Elsevier GmbH},

abstract = {The submitted work assumes that the abundance and diversity of endophytic and rhizosphere microorganisms co-existing with the halophytic plant Aster tripolium L. growing in a salty meadow in the vicinity of a soda factory (central Poland) represent unique populations of cultivable bacterial strains.Endophytic and rhizosphere bacteria were (i) isolated and identified based on 16S rDNA sequences; (ii) screened for nifH and acdS genes; and (iii) analyzed based on selected metabolic properties. Moreover, total microbial biomass and community structures of the roots (endophytes), rhizosphere and soil were evaluated using a cultivation-independent technique (PLFA) to characterize plant-microbial interactions under natural salt conditions.The identification of the isolated strains showed domination by Gram-positive bacteria (mostly Bacillus spp.) both in the rhizosphere (90.9%) and roots (72.7%) of A. tripolium. Rhizosphere bacterial strains exhibited broader metabolic capacities, while endophytes exhibited higher specificities for metabolic activity. The PLFA analysis showed that the total bacterial biomass decreased in the following order (rhizosphere < soil < endophytes) and confirmed the dominance of Gram-positive endophytic bacteria in the roots of the halophyte.The described strain collection provides a valuable basis for a subsequent applications of bacteria in improvement of site adaptation of plants in saline soils. © 2015 Elsevier GmbH.},

note = {46},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84945469350,

title = {Links in the functional diversity between soil microorganisms and plant communities during natural succession in coal mine spoil heaps},

author = { A. Markowicz and G. Woźniak and S. Borymski and Z. Piotrowska-Seget and D. Chmura},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84945469350&doi=10.1007%2fs11284-015-1301-3&partnerID=40&md5=ed2138c5482a3b345d7d8e3a0aef24b9},

doi = {10.1007/s11284-015-1301-3},

issn = {09123814},

year  = {2015},

date = {2015-01-01},

journal = {Ecological Research},

volume = {30},

number = {6},

pages = {1005-1014},

publisher = {Springer Tokyo},

abstract = {The successful establishment of vegetation, soil development and biogeochemical cycling during the restoration process of mine tailings requires a diverse and metabolically active microbial communities. The objective of this study was to test whether there is any link between the functional groups of both the dominant plant species and soil microbial communities on unreclaimed coal mine spoil heaps of different age located in the Silesian Upland (Poland). At each sampling site the dominant plant species were recorded and characterised based on their Raunkiaer’s life form, socio-ecological group and their potential to form mycorrhiza. The functional diversity of the plant-associated microbial communities was assessed using the microbial carbon-utilisation guilds generated using the Biolog method. We observed no differences in the microbial functional diversity, but a gradual increase in the plant functional diversity with the age of the heap. Our results indicate that trees, plants with the potential to form ectomycorrhiza, and deciduous plants strongly affected the carbon-utilisation profiles. The mean proportion of microbe guilds in dominant plant patches accounts for 60 % of the variance while the soil physicochemical parameters explained only 30 % of the variance. This suggest that in post-industrial habitats the biotic features of the soil substratum are more important for the vegetation development than the abiotic parameters. © 2015, The Author(s).},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84946717905,

title = {The relationship between successional vascular plant assemblages and associated microbial communities on coal mine spoil heaps},

author = { G. Woźniak and A. Markowicz and S. Borymski and Z. Piotrowska-Seget and D. Chmura and L. Besenyei},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946717905&doi=10.1556%2f168.2015.16.1.3&partnerID=40&md5=081e835c5d76e5bd2b3f851e1eb3c48b},

doi = {10.1556/168.2015.16.1.3},

issn = {15858553},

year  = {2015},

date = {2015-01-01},

journal = {Community Ecology},

volume = {16},

number = {1},

pages = {23-32},

publisher = {Akademiai Kiado Rt.},

abstract = {The aim of the study was to investigate the relationships between the vascular plant species and the associated soil microbial properties at various stages of vegetation development on unclaimed hard coal mine spoil heaps in Upper Silesia (south Poland). The spontaneous vegetation, soil chemistry as well as the activity and structure of microbial communities were recorded on this specific habitat. The colliery heaps were divided into four age classes and the plant species composition and cover abundance were recorded on established plots (2 m × 2 m). The soil microbial activity under the vegetation patches was assessed using fluorescein diacetate hydrolytic activity (FDHA) and the soil microbial biomass and community composition were determined by phospholipid fatty acid (PLFA) biomarkers. Total microbial biomass in soils from the older vegetation plots was significantly higher than those in soils from the younger plots. In all studied samples, microbial communities consisted primarily of bacteria with the dominance of Gram negative bacteria over Gram positive and aerobic microorganisms were more dominant than anaerobic ones. Statistical analysis revealed a correlation between the type of vegetation and microbial community structure.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84942428396,

title = {Endophytic bacteria and their role in environmental microbiology, medicine and industry [Bakterie endofityczne i ich znaczenie w mikrobiologii s̈rodowiskowej, medycynie i przemys̈le]},

author = { M. Pawlik and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942428396&partnerID=40&md5=05709f76f31dd8658cc135b2bf0a4e0a},

issn = {00794252},

year  = {2015},

date = {2015-01-01},

journal = {Postepy Mikrobiologii},

volume = {54},

number = {2},

pages = {115-122},

publisher = {Polish Society of Microbiologists},

abstract = {Endophytic bacteria have been known for more than 120 years. They live inside plant tissues without causing any apparent symptoms of disease or negative effects on the host. Each of the nearly 300 000 plant species that exist on the earth is host to one or more endophytes. Only a few of these plants have ever been completely studied relative to their endophytic biology. Consequently, the opportunity to find new and beneficial endophytic microorganisms among the diversity of plants in different ecosystems is considerable. During the long co-evolutionary process with their hosts, endophytes have developed many significant and novel characteristics. The relationships between plants and endophytic bacteria are very close. The endophytes which reside inside plant tissues can be classified as 'obligate' or 'facultative'. The first group is strictly dependent on the host plant for their growth and survival and transmission to other plants. The second group has a stage in their life cycle in which they are able to exist outside the host plant. Recent studies have shown that endophytes accelerate the adaptation of plants to unbalanced environmental conditions such as saline soil, drought, stress caused by pesticides, heavy metals or hydrocarbons. Therefore, the exploitation of the interaction of plant endophytes for the remediation of contaminated soils is a promising area; however, role of these microorganisms is still unclear.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84927623488,

title = {Biochemical and microbial soil functioning after application of the insecticide imidacloprid},

author = { M. Cycoń and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927623488&doi=10.1016%2fj.jes.2014.05.034&partnerID=40&md5=fdf788d03c908aee08a3494fd2e8132c},

doi = {10.1016/j.jes.2014.05.034},

issn = {10010742},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Environmental Sciences (China)},

volume = {27},

number = {C},

pages = {147-158},

publisher = {Chinese Academy of Sciences},

abstract = {Imidacloprid is one of the most commonly used insecticides in agricultural practice, and its application poses a potential risk for soil microorganisms. The objective of this study was to assess whether changes in the structure of the soil microbial community after imidacloprid application at the field rate (FR; 1mg/kg soil) and 10 times the FR (10× FR; 10mg/kg soil) may also have an impact on biochemical and microbial soil functioning. The obtained data showed a negative effect by imidacloprid applied at the FR dosage for substrate-induced respiration (SIR), the number of total bacteria, dehydrogenase (DHA), both phosphatases (PHOS-H and PHOS-OH), and urease (URE) at the beginning of the experiment. In 10× FR treated soil, decreased activity of SIR, DHA, PHOS-OH and PHOS-H was observed over the experimental period. Nitrifying and N2-fixing bacteria were the most sensitive to imidacloprid. The concentration of NO3- decreased in both imidacloprid-treated soils, whereas the concentration of NH4+ in soil with 10× FR was higher than in the control. Analysis of the bacterial growth strategy revealed that imidacloprid affected the r- or K-type bacterial classes as indicated also by the decreased eco-physiological (EP) index. Imidacloprid affected the physiological state of culturable bacteria and caused a reduction in the rate of colony formation as well as a prolonged time for growth. Principal component analysis showed that imidacloprid application significantly shifted the measured parameters, and the application of imidacloprid may pose a potential risk to the biochemical and microbial activity of soils. © 2014 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences.},

note = {37},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84924039820,

title = {Community structure of ammonia-oxidizing archaea and ammonia-oxidizing bacteria in soil treated with the insecticide imidacloprid},

author = { M. Cycoń and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924039820&doi=10.1155%2f2015%2f582938&partnerID=40&md5=45ca38ac6cb1636e9d52cd7f77c179a5},

doi = {10.1155/2015/582938},

issn = {23146133},

year  = {2015},

date = {2015-01-01},

journal = {BioMed Research International},

volume = {2015},

publisher = {Hindawi Publishing Corporation},

abstract = {The purpose of this experiment was to assess the effect of imidacloprid on the community structure of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in soil using the denaturing gradient gel electrophoresis (DGGE) approach. Analysis showed that AOA and AOB community members were affected by the insecticide treatment. However, the calculation of the richness (S) and the Shannon-Wiener index (H) values for soil treated with the field rate (FR) dosage of imidacloprid (1 mg/kg soil) showed no changes in measured indices for the AOA and AOB community members. In turn, the 10× FR dosage of insecticide (10 mg/kg soil) negatively affected the AOA community, which was confirmed by the decrease of the S and H values in comparison with the values obtained for the control soil. In the case of AOB community, an initial decline followed by the increase of the S and H values was obtained. Imidacloprid decreased the nitrification rate while the ammonification process was stimulated by the addition of imidacloprid. Changes in the community structure of AOA and AOB could be due to an increase in the concentration of N-NH4+, known as the most important factor which determines the contribution of these microorganisms to soil nitrification. © 2015 Mariusz Cycoń and Zofia Piotrowska-Seget.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84910056367,

title = {Changes in enzyme activities and microbial community structure in heavy metal-contaminated soil under in situ aided phytostabilization},

author = { D. Wasilkowski and A. Mrozik and Z. Piotrowska-Seget and J. Krzyżak and M. Pogrzeba and G.A. Płaza},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84910056367&doi=10.1002%2fclen.201300631&partnerID=40&md5=977a3d2d10c88755edc3d959ed2669b5},

doi = {10.1002/clen.201300631},

issn = {18630650},

year  = {2014},

date = {2014-01-01},

journal = {Clean - Soil, Air, Water},

volume = {42},

number = {11},

pages = {1618-1625},

publisher = {Wiley-VCH Verlag},

abstract = {The effect of lignite and lime on the structural biodiversity and enzyme activities of soil microorganisms in heavy metal-contaminated soil during an aided phytostabilization was studied. Over the experiment the amount of bioavailable fractions of Zn and Cd declined threefold and in the end reached the values of 87.8 and 4.2mgkg-1 for Zn and Cd, respectively. The activities of enzymes increased markedly in soil under aided phytostabilization. On the last sampling day the activities of dehydrogenase, alkaline and acid phosphatase, as well urease, were 15-, 8-, 4-, and 19-fold higher as compared to the initial values. The analyses of fatty acid methyl ester and phospholipid fatty acid (PLFA) patterns showed a shift in the structure of microbial populations in the treated soil as compared to untreated soil. In general, the microbial biomass of total, Gram-positive and Gram-negative bacteria (GNBB), as well actinomycetes and fungi (FB), was higher in the soil under aided phytostabilization in comparison with control soil. The highest biomass increase from 2.3 to 2.9 and from 0.4 to 0.8nmolPLFAg-1 soil (dry weight), was found for GNBB and FB, respectively. Phytostabilization supported by lignite and lime appeared to be an effective method to decrease Zn and Cd content in heavy metal-contaminated soil. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84942902179,

title = {Fitoremediacja terenów skażonych związkami ropopochodnymi z wykorzystaniem bakterii endofitycznych [Phytoremediation of petroleum hydrocarbons-contaminated soils by using endophytic bacteria]},

author = { M. Kukla and B. Cania and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942902179&doi=10.12916%2fprzemchem.2014.355&partnerID=40&md5=c4c47f68c81138f33496465cb92979de},

doi = {10.12916/przemchem.2014.355},

issn = {00332496},

year  = {2014},

date = {2014-01-01},

journal = {Przemysl Chemiczny},

volume = {93},

number = {3},

pages = {355-359},

publisher = {Wydawnictwo SIGMA-NOT},

abstract = {[No abstract available]},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84922754626,

title = {Molecular basis of bacterial resistance to ampicillin [Molekularne podstawy bakteryjnej opornos̈ci na ampicylinę]},

author = { K. Bondarczuk and S. Sułowicz and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84922754626&partnerID=40&md5=8bd5979c0fea5faf79ba888c27098f8d},

issn = {00794252},

year  = {2014},

date = {2014-01-01},

journal = {Postepy Mikrobiologii},

volume = {53},

number = {4},

pages = {360-365},

publisher = {Polish Society of Microbiologists},

abstract = {β-lactam antibiotics are regularly used in medicine to control bacterial infections. However the overuse of these compounds in many human activities has led to dissemination of resistance determinants among pathogenic strains. Several mechanisms underlining β-lactam resistance, e.g. enzymatic inactivation, PBP modification, active efflux, and finally the reduction of cell wall permeability have been identified. Understanding of such mechanisms is the first step to overcome the resistance. Ampicillin was one of the first broad-spectrum amino penicillins introduced to therapy. Nowadays, five decades after the introduction of the antibiotic, ampicillin-resistant microbes are isolated all over the world. Current knowledge about the mechanisms involved in ampicillin resistance is presented in this review.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84919597980,

title = {Diversity of endophytic bacteria in Lolium perenne and their potential to degrade petroleum hydrocarbons and promote plant growth},

author = { M. Kukla and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84919597980&doi=10.1016%2fj.chemosphere.2014.05.055&partnerID=40&md5=4f0ba66db9a3d4853e04b6239853f7e5},

doi = {10.1016/j.chemosphere.2014.05.055},

issn = {00456535},

year  = {2014},

date = {2014-01-01},

journal = {Chemosphere},

volume = {117},

number = {1},

pages = {40-46},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to assess the ability of twenty-nine endophytic bacteria isolated from the tissues of ryegrass (Lolium perenne L.) to promote plant growth and the degradation of hydrocarbon. Most of the isolates belonged to the genus Pseudomonas and showed multiple plant growth-promoting abilities. All of the bacteria that were tested exhibited the ability to produce indole-3-acetic acid and were sensitive to streptomycin. These strains were capable of phosphate solubilization (62%), cellulolytic enzyme production (62%), a capacity for motility (55%) as well as for the production of siderophore (45%), ammonium (41%) and hydrogen cyanide (38%). Only five endophytes had the emulsification ability that results from the production of biosurfactants. The 1-aminocyclopropane-1-carboxylate deaminase (ACCD) gene (acdS) was found in ten strains. These bacteria exhibited ACCD activities in the range from 1.8 to 56.6μmol of α-ketobutyrate mg-1h-1, which suggests that these strains may be able to modulate ethylene levels and enhance plant growth. The potential for hydrocarbon degradation was assessed by PCR amplification on the following genes: alkH, alkB, C23O, P450 and pah. The thirteen strains that were tested had the P450 gene but the alkH and pah genes were found only in the Rhodococcus fascians strain (L11). Four endophytic bacteria belonging to Microbacterium sp. and Rhodococcus sp. (L7; S12; S23; S25) showed positive results for the alkB gene. © 2014 Elsevier Ltd.},

note = {64},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84905699798,

title = {Rhizosphere of metallophytes and its role in bioremediation of heavy metals},

author = { S. Borymski and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84905699798&partnerID=40&md5=72c239b2e87f24d70a41f7d8a529b547},

issn = {00092886},

year  = {2014},

date = {2014-01-01},

journal = {Chemik},

volume = {68},

number = {6},

pages = {554-559},

publisher = {Chem Press},

abstract = {[No abstract available]},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84905679951,

title = {Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil},

author = { M. Pacwa-Płociniczak and G.A. Płaza and A. Poliwoda and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84905679951&doi=10.1007%2fs11356-014-2872-1&partnerID=40&md5=ec6d3cfa413b19cb9a07ef1ebb31b50d},

doi = {10.1007/s11356-014-2872-1},

issn = {09441344},

year  = {2014},

date = {2014-01-01},

journal = {Environmental Science and Pollution Research},

volume = {21},

number = {15},

pages = {9385-9395},

publisher = {Springer Verlag},

abstract = {The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27; 39; 27 and 13 % of hydrocarbons added to culture medium were degraded; respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils. © 2014 The Author(s).},

note = {68},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84902385351,

title = {Microbial diversity in waters, sediments and microbial mats evaluated using fatty acid-based methods},

author = { A. Mrozik and A. Nowak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84902385351&doi=10.1007%2fs13762-013-0449-z&partnerID=40&md5=b6b64e15d99cba8ebb6301231b950f3d},

doi = {10.1007/s13762-013-0449-z},

issn = {17351472},

year  = {2014},

date = {2014-01-01},

journal = {International Journal of Environmental Science and Technology},

volume = {11},

number = {5},

pages = {1487-1496},

publisher = {Center for Environmental and Energy Research and Studies},

abstract = {The review summarises recent advances towards a greater comprehensive assessment of microbial diversity in aquatic environments using the fatty acid methyl esters and phospholipid fatty acids approaches. These methods are commonly used in microbial ecology because they do not require the culturing of micro-organisms, are quantitative and reproducible and provide valuable information regarding the structure of entire microbial communities. Because some fatty acids are associated with taxonomic and functional groups of micro-organisms, they allow particular groups of micro-organisms to be distinguished. The integration of fatty acid-based methods with stable isotopes, RNA and DNA analyses enhances our knowledge of the role of micro-organisms in global nutrient cycles, functional activity and phylogenetic lineages within microbial communities. Additionally, the analysis of fatty acid profiles enables the shifts in the microbial diversity in pristine and contaminated environments to be monitored. The main objective of this review is to present the use of lipid-based approaches for the characterisation of microbial communities in water columns, sediments and biomats. © 2013 The Author(s).},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84902373720,

title = {Enhancement of deltamethrin degradation by soil bioaugmentation with two different strains of Serratia marcescens},

author = { M. Cycoń and A. Żmijowska and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84902373720&doi=10.1007%2fs13762-013-0322-0&partnerID=40&md5=a3c8cf0fdd4ca7f55eeb3a6d4cefe626},

doi = {10.1007/s13762-013-0322-0},

issn = {17351472},

year  = {2014},

date = {2014-01-01},

journal = {International Journal of Environmental Science and Technology},

volume = {11},

number = {5},

pages = {1305-1316},

publisher = {Center for Environmental and Energy Research and Studies},

abstract = {Deltamethrin is one of the most commonly used pyrethroid in agricultural practice in different geographic regions of the world. It is detected in many environments, especially in soil and water, and can exhibit toxic effect to human and other organisms. In this study, we describe two bacterial strains DeI-1 and DeI-2, isolated from soil, and both identified as Serratia marcescens based on profile of the fatty acid methyl esters, biochemical test, and 16S RNA gene analysis, which were shown to efficiently degrade deltamethrin. Degradation of deltamethrin in mineral salt medium (50 mg l -1 ) proceeded by strains DeI-1 or DeI-2 reached the values of 88.3 or 82.8 % after 10 days, and DT50 was 2.8 or 4.0 days, respectively. Bioaugmentation of deltamethrin-contaminated non-sterile soils (100 mg kg -1 ) with strains DeI-1 or DeI-2 (3 × 10 6 cells g -1 of soil) enhanced the disappearance rate of pyrethroid, and its DT50 was reduced by 44.9, 33.1, 44.4, and 58.2 days or 39.1, 25.8, 35.6, and 46.0 days in sandy, sandy loam, silty loam, and silty soils, respectively, in comparison with non-sterile soils with only indigenous microflora. The three-way ANOVA indicated that DT50 of deltamethrin was significantly (P < 0.01) affected by soil type, microflora presence, and inoculum, and the interaction between these factors. Generally, the lower content of clay and organic carbon in soil, the higher degradation rate of deltamethrin was observed. Obtained results show that both strains of S. marcescens may possess potential to be used in bioremediation of deltamethrin-contaminated soils. © 2013 The Author(s).},

note = {75},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84891618950,

title = {Lead resistance in micro-organisms},

author = { A. Jarosławiecka and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891618950&doi=10.1099%2fmic.0.070284-0&partnerID=40&md5=0eb60c3171b8f8f2d8aef9fd3b4067a4},

doi = {10.1099/mic.0.070284-0},

issn = {13500872},

year  = {2014},

date = {2014-01-01},

journal = {Microbiology (United Kingdom)},

volume = {160},

number = {PART 1},

pages = {12-25},

abstract = {Lead (Pb) is an element present in the environment that negatively affects all living organisms. To diminish its high toxicity, micro-organisms have developed several mechanisms that allow them to survive exposure to Pb(II). The main mechanisms of lead resistance involve adsorption by extracellular polysaccharides, cell exclusion, sequestration as insoluble phosphates, and ion efflux to the cell exterior. This review describes the various lead resistance mechanisms, and the regulation of their expression by lead binding regulatory proteins. Special attention is given to the Pbr system from Cupriavidus metallidurans CH34, which involves a unique mechanism combining efflux and lead precipitation. © 2014 SGM.},

note = {127},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84886257604,

title = {Imidacloprid induces changes in the structure, genetic diversity and catabolic activity of soil microbial communities},

author = { M. Cycoń and A. Markowicz and S. Borymski and M. Wójcik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886257604&doi=10.1016%2fj.jenvman.2013.09.041&partnerID=40&md5=d0547dc811446de6ec4a2d491dabe5c2},

doi = {10.1016/j.jenvman.2013.09.041},

issn = {03014797},

year  = {2013},

date = {2013-01-01},

journal = {Journal of Environmental Management},

volume = {131},

pages = {55-65},

abstract = {This is the first report describing the effect of imidacloprid applied at field rate (FR; 1mg/kg of soil) and 10 times the FR (10*FR; 10mg/kg of soil) on the structural, genetic and physiological diversity of soil bacterial community as determined by the phospholipid fatty acid (PLFA), the denaturing gradient gel electrophoresis (DGGE), and the community level physiological profile (CLPP) approaches. PLFA profiles showed that imidacloprid significantly shifted the microbial community structure and decreased the biomass of the total, bacterial and fungal PLFAs, however, this effect was transient at the FR dosage. The alterations in DGGE patterns caused by imidacloprid application, confirmed considerable changes in the overall richness and diversity of dominant bacteria. Although, as a result of imidacloprid application, the metabolic activity of microbial communities was generally lower, the richness and functional biodiversity of the soil microbial community were not negatively affected. In general, the analysis of the variance indicated that the measured parameters were significantly affected by treatment and the incubation time, however, the incubation time effect explained most of the observed variance. Imidacloprid degradation and the appearance of some new bands in DGGE profiles suggest the evolution of bacteria capable of degrading imidacloprid among indigenous microflora. © 2013 Elsevier Ltd.},

note = {68},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84890633918,

title = {Molecular basis of active copper resistance mechanisms in Gram-negative bacteria},

author = { K. Bondarczuk and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84890633918&doi=10.1007%2fs10565-013-9262-1&partnerID=40&md5=0783c8716bf8b7a798cfd3b7fd489e49},

doi = {10.1007/s10565-013-9262-1},

issn = {07422091},

year  = {2013},

date = {2013-01-01},

journal = {Cell Biology and Toxicology},

volume = {29},

number = {6},

pages = {397-405},

abstract = {Copper is a metallic element that is crucial for cell metabolism; however, in extended concentrations, it is toxic for all living organisms. The dual nature of copper has forced organisms, including bacteria, to keep a tight hold on cellular copper content. This challenge has led to the evolution of complex mechanisms that on one hand enable them to deliver the essential element and on the other to protect cells against its toxicity. Such mechanisms have been found in both eukaryotic and prokaryotic cells. In bacteria a number of different systems such as extra- and intracellular sequestration, enzymatic detoxification, and metal removal from the cell enabling them to survive in the presence of high concentration of copper have been identified. Gram-negative bacteria, due to their additional compartment, need to deal with both cytoplasmic and periplasmic copper. Therefore, these bacteria have evolved intricate and precisely regulated systems which interact with each other. In this review the active mechanisms of copper resistance at their molecular level are discussed. © 2013 The Author(s).},

note = {127},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84886592804,

title = {Cadmium increases catechol 2,3-dioxygenase activity in Variovorax sp. 12S, a metal-tolerant and phenol-degrading strain},

author = { K.T. Hupert-Kocurek and A. Saczyńska and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886592804&doi=10.1007%2fs10482-013-9997-y&partnerID=40&md5=ba31ec309fe2261131dc2c0cd59e1f3f},

doi = {10.1007/s10482-013-9997-y},

issn = {00036072},

year  = {2013},

date = {2013-01-01},

journal = {Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology},

volume = {104},

number = {5},

pages = {845-853},

abstract = {A Gram-negative bacterium, designated as strain 12S, was isolated from a heavy metal-polluted soil. According to the biochemical characteristics, FAME analysis, and 16S rRNA gene sequence analysis, the isolated strain was identified as Variovorax sp. 12S. In the presence of 0.1 mM cadmium, 12S was able to completely utilize up to 1.5 mM of phenol as the sole carbon and energy source in an MSM-TRIS medium. Degradation of phenol was accompanied by a slow bacterial growth rate and an extension of the lag phase. The cells grown on phenol showed catechol 2,3-dioxygenase (C23O) activity. The activity of C23O from 12S cultivated in medium with Cd2+ was almost 20 % higher than in the control. Since environmental contamination with aromatic compounds is often accompanied by the presence of heavy metals, Variovorax sp. 12S and its C23O appear to be very powerful and useful tools in the biotreatment of wastewaters and soil decontamination. © 2013 Springer Science+Business Media Dordrecht.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84882968565,

title = {Structural and functional diversity of bacterial community in soil treated with the herbicide napropamide estimated by the DGGE, CLPP and r/K-strategy approaches},

author = { M. Cycoń and A. Markowicz and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84882968565&doi=10.1016%2fj.apsoil.2013.07.015&partnerID=40&md5=898ca9abba807ecd826c4d67af6d4af7},

doi = {10.1016/j.apsoil.2013.07.015},

issn = {09291393},

year  = {2013},

date = {2013-01-01},

journal = {Applied Soil Ecology},

volume = {72},

pages = {242-250},

abstract = {Napropamide is one of the most commonly used herbicide in agricultural practice and can exhibit toxic effect to soil microorganisms. Therefore, the main objective of this study was to examine the genetic and functional diversity of microbial communities in soil treated with napropamide at field rate (FR; 2.25mgkg-1 of soil) and 10 times the FR (10×FR; 22.5mgkg-1 of soil) by the denaturing gradient gel electrophoresis (DGGE) and the community level physiological profile (CLPP) methods. In addition, the r/K-strategy approach was used to evaluate the effect of this herbicide on the community structure of the culturable soil bacteria. DGGE patterns revealed that napropamide affected the structure of microbial community; however, the richness (S) and genetic diversity (H) values indicated that the FR dosage of napropamide experienced non-significant changes. In turn, the 10×FR dosage of herbicide caused significant changes in the S and H values of dominant soil bacteria. DGGE profiles suggest an evolution of bacteria capable of degrading napropamide among indigenous microflora. Analysis of the CLPPs indicated that the catabolic activity of microbial community expressed as AWCD (average well-color development) was temporary positively affected after napropamide application and resulted in an increase of the substrate richness (SR) as well as functional biodiversity (H) values. Analysis of the bacterial growth strategy revealed that napropamide affected the r- or K-type bacterial classes (ecotypes). In treated-soil samples K-strategists dominated the population, as indicated by the decreased ecophysiological (EP) index. Napropamide significantly affected the physiological state of culturable bacteria and caused a reduction in the rate of colony formation as well as a prolonged time of growth rate. Obtained results indicate that application of napropamide may poses a potential risk for soil functioning. © 2013 Elsevier B.V.},

note = {26},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84874432052,

title = {Short-term effects of the herbicide napropamide on the activity and structure of the soil microbial community assessed by the multi-approach analysis},

author = { M. Cycoń and M. Wójcik and S. Borymski and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874432052&doi=10.1016%2fj.apsoil.2013.01.014&partnerID=40&md5=9fae37ddf683b5976bef5d1883ece32c},

doi = {10.1016/j.apsoil.2013.01.014},

issn = {09291393},

year  = {2013},

date = {2013-01-01},

journal = {Applied Soil Ecology},

volume = {66},

pages = {8-18},

abstract = {Napropamide is one of the most commonly used herbicide in agricultural practice and its continuously application poses a potential risk for non-target soil microorganisms. Therefore, the objective of this study was to assess the impact of napropamide, applied at the field rate (FR; 2.25mgkg-1 of soil) and 10 times the FR (10*FR; 22.5mgkg-1 of soil) on soil microorganisms. To ascertain this impact, substrate-induced respiration (SIR), dehydrogenase (DHA), acid and alkaline phosphatases (PHOS-H and PHOS-OH), urease (URE) activities and changes in concentrations of NO3- and NH4+ ions were determined. In addition, numbers of total bacteria and bacteria involved in soil nitrogen transformation were enumerated. A phospholipid fatty acid (PLFA) method was used to assess changes in the structure of soil microbial communities. Results showed negative effect of napropamide applied at the FR for SIR, the number of total bacteria, DHA, both PHOS, and URE at the beginning of the experiment. In 10*FR treated soil, a decreased activity of SIR, DHA, PHOS-OH and PHOS-H was observed over the experimental period. Nitrifying and N2-fixing bacteria appeared to be the most sensitive to napropamide. The concentration of NO3- decreased in both napropamide-treated soils, whereas the concentration of NH4+ on day 28 in soil with 10*FR was 5 times higher than in the control. Analysis of the PLFA profiles showed that napropamide decreased the biomass of total, bacterial and fungal PLFAs on day 1, while at the end of the experiment in the soil treated with FR dosage of napropamide biomass of total, Gram-negative bacteria and fungi was significantly higher than those observed in the control. A principal component analysis of the PLFAs showed that napropamide application significantly shifted the microbial community structure on days 1 and 14. The degradation kinetics data showed that napropamide degradation by soil autochthonous microorganisms was relatively slow. The results indicated that a broad spectrum of analyze gives a better insight into the true effects of napropamide on soil microorganisms than the single assays. © 2013 Elsevier B.V.},

note = {75},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84872471996,

title = {Biodegradation and bioremediation potential of diazinon-degrading Serratia marcescens to remove other organophosphorus pesticides from soils},

author = { M. Cycoń and A. Żmijowska and M. Wójcik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872471996&doi=10.1016%2fj.jenvman.2012.12.031&partnerID=40&md5=54ce89cd2b614436e944da2c20a9ed74},

doi = {10.1016/j.jenvman.2012.12.031},

issn = {03014797},

year  = {2013},

date = {2013-01-01},

journal = {Journal of Environmental Management},

volume = {117},

pages = {7-16},

abstract = {The ability of diazinon-degrading Serratia marcescens to remove organophosphorus pesticides (OPPs), i.e. chlorpyrifos (CP), fenitrothion (FT), and parathion (PT) was studied in a mineral salt medium (MSM) and in three soils of different characteristics. This strain was capable of using all insecticides at concentration of 50 mg/l as the only carbon source when grown in MSM, and 58.9%, 70.5%, and 82.5% of the initial dosage of CP, FT, and PT, respectively was degraded within 14 days. The biodegradation experiment showed that autochthonous microflora in all soils was characterized by a degradation potential of all tested OPPs; however, the initial lag phases for degradation of CP and FT, especially in sandy soil, were observed. During the 42-day experiment, 45.3%, 61.4% and 72.5% of the initial dose of CP, FT, and PT, respectively, was removed in sandy soil whereas the degradation of CP, FT, and PT in the same period, in sandy loam and silty soils reached 61.4%, 79.7% and 64.2%, and 68.9%, 81.0% and 63.6%, respectively. S. marcescens introduced into sterile soils showed a higher degradation potential (5-13%) for OPPs removal than those observed in non-sterile soil with naturally occurring attenuation. Inoculation of non-sterile soils with S. marcescens enhanced the disappearance rates of all insecticides, and DT50 for CP, FT, and PT was reduced by 20.7, 11.3 and 13.0 days, and 11.9, 7.0 and 8.1 days, and 9.7, 14.5 and 12.6 days in sandy, sandy loam, and silty soils, respectively, in comparison with non-sterile soils with only indigenous microflora. This ability of S. marcescens makes it a suitable strain for bioremediation of soils contaminated with OPPs. © 2013 Elsevier Ltd.},

note = {105},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84876432490,

title = {The effect of soil bioaugmentation with strains of Pseudomonas on Cd, Zn and Cu uptake by Sinapis alba L.},

author = { T. Płociniczak and M. Kukla and R. Watroba and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876432490&doi=10.1016%2fj.chemosphere.2013.03.008&partnerID=40&md5=74246e8c748c10f265f43a70ea814c1c},

doi = {10.1016/j.chemosphere.2013.03.008},

issn = {00456535},

year  = {2013},

date = {2013-01-01},

journal = {Chemosphere},

volume = {91},

number = {9},

pages = {1332-1337},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to assess the ability of selected metal resistant strains of the Pseudomonas genus to increase Zn, Cd and Cu uptake by the metalophyte Sinapis alba L. under laboratory conditions. Moreover, the mechanisms of the plant growth promotion in the tested strains and their impact on the shoots and roots of white mustard biomass were examined. Soil inoculation with the tested strains resulted in higher concentrations of Zn, Cd and Cu in the shoots and roots of the plants in comparison with those grown in non-inoculated soil. The highest phytoextraction enhancement was caused by Pseudomonas fluorescens MH15 which increased Zn, Cd and Cu accumulation in shoot tissue by 60%, 96% and 31%, respectively, in comparison with control plants. Moreover, all the tested strains also exhibited a significant increase of Cd translocation from roots to shoots of the white mustard. Three Pseudomonas putida (MH3; MH6; MH7) and two P. fluorescens biotype G and C (MH9 and MH15; respectively) strains had the ability to produce siderophore, 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid as well as hydrocyanic acid. Additionally, P. putida strains were also capable of solubilizing inorganic phosphate. The ability of the tested strains to increase the metal uptake in white mustard and their plant growth-promoting properties make them good candidates for supporting heavy metal phytoextraction as well as for plant growth promoting. © 2013 Elsevier Ltd.},

note = {42},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84871021485,

title = {Characterization of Enterobacter intermedius MH8b and its use for the enhancement of heavy metals uptake by Sinapis alba L.},

author = { T. Płociniczak and A. Sinkkonen and M. Romantschuk and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84871021485&doi=10.1016%2fj.apsoil.2012.09.009&partnerID=40&md5=9d1ed1c9a279c2356085e1ec5be702a4},

doi = {10.1016/j.apsoil.2012.09.009},

issn = {09291393},

year  = {2013},

date = {2013-01-01},

journal = {Applied Soil Ecology},

volume = {63},

pages = {1-7},

abstract = {The aim of this study was to test whether the multiple-tolerant bacterium Enterobacter intermedius MH8b isolated from metal-contaminated soil is able to promote plant growth and enhance Zn, Cd and Cu uptake by Sinapis alba L. under laboratory conditions. Additionally, the mechanisms of the plant growth promotion and the ecological consequences of bioaugmentation were examined. MH8b exhibited the ability to produce 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid and hydrocyanic acid and was also able to solubilize inorganic phosphate. Soil inoculation with MH8b significantly increased plant biomass as well as the accumulation of Zn (32%) and Cd (94%) in plant shoots. DGGE and PLFA analysis revealed that the introduced MH8b had only a short-term influence on the structure of indigenous microbial communities. The plant growth-promoting properties of MH8b and its ability to increase the metal uptake connected with its long-term survival and its short-term impact on autochthonous microflora make the strain a good candidate for the promotion of plant growth and phytoremediation efficiency. © 2012 Elsevier B.V.},

note = {47},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84861413257,

title = {A broad-spectrum analysis of the effects of teflubenzuron exposure on the biochemical activities and microbial community structure of soil},

author = { M. Cycoń and M. Wójcik and S. Borymski and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861413257&doi=10.1016%2fj.jenvman.2012.05.002&partnerID=40&md5=733ccdee444ccbb36f4cb29a151fece2},

doi = {10.1016/j.jenvman.2012.05.002},

issn = {03014797},

year  = {2012},

date = {2012-01-01},

journal = {Journal of Environmental Management},

volume = {108},

pages = {27-35},

abstract = {We evaluated the response of soil bacteria to applications of the insecticide teflubenzuron at the field rate dosage (FR; 0.15 mg/kg of soil) and at a higher dosage (10*FR; 1.5 mg/kg of soil). When applied at the FR dosage, teflubenzuron had no effect on several biochemical parameters of the soil, including substrate-induced respiration (SIR), dehydrogenase (DHA) and phosphatase activities (PHOS), and N-NO3- and N-NH4+ concentrations. Additionally, no differences were observed in the culturable fraction of the soil bacteria (the number of heterotrophic; nitrifying; denitrifying and N2-fixing bacteria; the growth strategy; the ecophysiological and colony development indices; and the physiological state). In contrast, treatment with the 10*FR dosage of the insecticide significantly increased SIR, DHA, PHOS and N-NH4+ levels and the number of heterotrophic and denitrifying bacteria. Decreases in urease activity (URE) and the number of nitrifying and N2-fixing bacteria were also observed. A phospholipid fatty acid (PLFA) method-based analysis of the entire soil microorganism population revealed that teflubenzuron treatment affected the total fatty acid level as well as those considered to be of Gram-positive bacteria, Gram-negative bacteria and fungi. This effect was observed on days 1 and 14 post-treatment. A principal component analysis (PCA) of the PLFAs showed that teflubenzuron treatment significantly shifted the microbial community structure; however, all of the observed effects were transient. Studies on the degradation of teflubenzuron revealed that this process is characterised by a short lag phase and a rate constant (k) of 0.020/day. This degradation rate follows first-order kinetics, and the DT50 was 33.5 days. This is the first study that thoroughly examines the functional and structural status of both the culturable and non-culturable fractions of the soil microbial community after teflubenzuron application. © 2012 Elsevier Ltd.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84863852273,

title = {GFP-tagged multimetal-tolerant bacteria and their detection in the rhizosphere of white mustard},

author = { Z. Piotrowska-Seget and G. Beściak and T. Bernaś and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863852273&doi=10.1007%2fs13213-011-0292-8&partnerID=40&md5=7b1bc62f67b5bb869c8f7edaaabd53ae},

doi = {10.1007/s13213-011-0292-8},

issn = {15904261},

year  = {2012},

date = {2012-01-01},

journal = {Annals of Microbiology},

volume = {62},

number = {2},

pages = {559-567},

abstract = {The introduction of rhizobacteria that tolerate heavy metals is a promising approach to support plants involved in phytoextraction and phytostabilisation. In this study, soil of a metal-mine wasteland was analyzed for the presence of metal-tolerant bacterial isolates, and the tolerance patterns of the isolated strains for a number of heavy metals and antibiotics were compared. Several of the multimetal-tolerant strains were tagged with a broad host range reporter plasmid (i.e. pPROBE-NT) bearing a green fluorescent protein marker gene (gfp). Overall, the metaltolerant isolates were predominately Gram-negative bacteria. Most of the strains showed a tolerance to five metals (Zn; Cu; Ni; Pb and Cd), but with differing tolerance patterns. From among the successfully tagged isolates, we used the transconjugant Pseudomonas putida G25 (pPROBE-NT) to inoculate white mustard seedlings. Despite a significant decrease in transconjugant abundance in the rhizosphere, the gfp-tagged cells survived on the root surfaces at a level previously reported for root colonisers. © The Author(s) 2011.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84861108850,

title = {Mineralization dynamics of chlormequat chloride (CCC) in soils of different textures},

author = { M. Cycoń and A. Lewandowska and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861108850&partnerID=40&md5=032689df0879a99ef90a877a3941e136},

issn = {12301485},

year  = {2012},

date = {2012-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {21},

number = {3},

pages = {595-602},

abstract = {The aim of this study was to evaluate the mineralization rates of chlormequat chloride (CCC) in soils of different textures from measurements of 14CO2 evolution over a period of 100 days. The level of extractable and bound residual 14C in the soils at the end of the experiment was also determined. The degradation pattern of CCC was similar in all soil types and degree of its mineralization ranged from 53.4 to 55.7% over a period of 100 days. This process in all soils was described by first-order kinetics and CCC was mineralized with a similar constant rate (k) on the level of 0.029-0.034 day-1, and DT50 ranged from 67.7 days for sandy soil to 71.0 days for silt loam soil. Evaporation was not significant in the removal of CCC from soils, and the total amount of 14C-volatile organic compounds recovered at the end of the trials was similar to the background level. The level of 14C-extractable residues was low and reached the maximum value of 7.2% of the introduced radioactivity for sand and sandy loam soils. Levels of 14C-bound residues were higher in all soil types. The highest value (39%) was obtained for silt soil, while in the remaining soils it was lower and ranged from 30 to 33.5% of the introduced radioactivity. The relatively large amount of bound residues of CCC is probably connected with a strong affinity for soil components despite of its high water solubility and potential to be leached.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84879297069,

title = {Study of antifungal activity of Bacilli species cultured on agro-industrial wastes [Wstępne badania właściwości przeciwgrzybowych bakterii z rodzaju Bacillus rosnących na odpadach z przemysłu rolno-spożywczego]},

author = { G.A. Płaza and E. Król and M. Pacwa-Płociniczak and Z. Piotrowska-Seget and L. Robin Brigmon},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84879297069&partnerID=40&md5=959fe23b8951ed25890f7221c8733828},

issn = {16440692},

year  = {2012},

date = {2012-01-01},

journal = {Acta Scientiarum Polonorum, Hortorum Cultus},

volume = {11},

number = {5},

pages = {169-182},

publisher = {Wydawnictwo Akad Rolniczej W Lublinie},

abstract = {The three Bacillus species isolated from petroleum refinery waste were examined for antifungal activity on brewery effluents and molasses for biotechnological applications. Bacillus strains were identified by three different methods: 16S rRNA gene sequences, BIOLOG system and fatty acid analysis (FAME). The results demonstrated the ability of all three Bacillus strains cultured on brewery effluents and molasses to inhibit mycelial growth of the 10 tested fungi to varying degrees measured by agar plate inhibition assays. Fungi inhibited to the greatest degree as measured by the zones of inhibition were Botrytis cinerea A 258, Phomopsis viticola W 977, Septoria carvi K 2082, Colletotrichum gloeosporioides A 259, Phoma complanata A 233 and Phoma exigua var. exigua A 175. It was also observed that the fungal mycelial growth was inhibited by the cell-free supernatants, indicating lipoprotein-like activity of antifungal agents (mainly biosurfactants). Tested fungi were most sensitive to the Bacilli supernatants obtained from the molasses cultures including: B. cinerea A 258, R. solani W 70, S. sclerotiorum K 2291, Phomopsis diachenii K 657, C. dematium K 425, P. complanata A 233, P. exigua var. exigua A 175. In the previous study it was shown that Bacillus species produced biosurfactants. Application of natural products such as these Bacillus species or their byproducts may be a new approach to phytopathogen control therefore reducing the need for fungicides.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-81255171446,

title = {Enhancement of phenol degradation by soil bioaugmentation with Pseudomonas sp. JS150},

author = { A. Mrozik and S. Miga and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-81255171446&doi=10.1111%2fj.1365-2672.2011.05140.x&partnerID=40&md5=62dbe74c7fbd217ae871ee2502a2fc9f},

doi = {10.1111/j.1365-2672.2011.05140.x},

issn = {13645072},

year  = {2011},

date = {2011-01-01},

journal = {Journal of Applied Microbiology},

volume = {111},

number = {6},

pages = {1357-1370},

abstract = {Aims: To test whether bioaugmentation with genetically modified Pseudomonas sp. JS150 strain could be used to enhance phenol degradation in contaminated soils. Methods and Results: The efficiency of phenol removal, content of humic carbon, survival of inoculant, number of total culturable autochthonous bacteria and changes in fatty acid methyl esters (FAME) profiling obtained directly from soils were examined. Bioaugmentation significantly accelerated phenol biodegradation rate in tested soils. Phenol applied at the highest concentration (5·0mgg -1 soil) was completely degraded in clay soil (FC) within 65days, whereas in sand soil (FS) within 72days. In comparison, phenol biodegradation proceeded for 68 and 96days in nonbioaugmented FC and FS soils, respectively. The content of humic carbon remained at the same level at the beginning and the end of incubation time in all soil treatments. The number of introduced bacteria (2·50×10 9g -1 soil) markedly decreased during the first 4 or 8days depending on contamination level and type of soil; however, inoculant survived over the experimental period of time. Analysis of FAME patterns indicated that changes in the percentages of cyclopropane fatty acids 17:0cy and 19:0cyω10c and branched fatty acids might be useful markers for monitoring the progress of phenol removal from soil. Conclusions: It was confirmed that soil bioaugmentation with Pseudomonas sp. JS150 significantly enhanced soil activity towards phenol degradation. Cyclopropane and branched fatty acids were sensitive probes for degree of phenol utilization. Significance and Impact of the Study: In future, genetically modified Pseudomonas sp. JS150 strain could be of use in the bioaugmentation of phenol-contaminated areas. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-80054732959,

title = {Agroindustrial wastes as unconventional substrates for growing of bacillus strains and production of biosurfactant},

author = { G.A. Płaza and M. Pacwa-Płociniczak and Z. Piotrowska-Seget and K. Jangid and K.A. Wilk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80054732959&partnerID=40&md5=ad93e3a9b96ab17ae6df17304fa3f960},

issn = {03248828},

year  = {2011},

date = {2011-01-01},

journal = {Environment Protection Engineering},

volume = {37},

number = {3},

pages = {63-71},

abstract = {The study was aimed at the development of economical methods for biosurfactant production by the use of unconventional substrates. The research investigated the potential of utilising agroindustrial wastes to replace synthetic media for cultivation of Bacillus strains and biosurfactant production. In total, 21 of the waste products from dairy, sugar, fatty, and fruit and vegetable processing industries, breweries, distillery were examined. Three bacterial strains were identified by 16S rRNA gene sequencing: Bacillus subtilis (I'-1a), Bacillus sp. (T-1), Bacillus sp. (T'-1). Biosurfactant production was examined in an indirect way by measuring of surface tension (ST), blood agar lysis, oil spreading and drop collapsing tests. The best unconventional substrates for bacteria growing and biosurfactant production at 30 °C under aerobic conditions were molasses, brewery effluents, and fruit and vegetable decoction from the processing factory.},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-80052943328,

title = {Comparison of mineralization dynamics of 2,4-Dichlorophenoxyacetic acid (2,4-D) and 4-Chloro-2-Methylphenoxyacetic acid (MCPA) in soils of different textures},

author = { M. Cycoń and A. Lewandowska and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052943328&partnerID=40&md5=06b5ce208c256bfe92083f8fbe916cac},

issn = {12301485},

year  = {2011},

date = {2011-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {20},

number = {2},

pages = {293-301},

abstract = {The degradation behaviour of 2,4-D and MCPA in four types of soil were determined from measurements of 14CO 2 evolution over a period of 100 days. The total 14C-organic volatile compounds evaporated from the soils during the experimental period and the residual 14C in the soils at the end of the incubation period was also determined. The degree of mineralization was different for tested pesticides, and did not exceed 30% for 2,4-D or 46% for MCPA. The greatest mineralization of 2,4-D occurred in sandy soils containing the least amount of organic carbon, while in the case of MCPA, the highest level of mineralization was observed in loamy sand and silt loam soils. Volatilization was the most important mechanism of 2,4-D loss from soils and accounted for 46.6% of the total applied dose for sandy loam soil. The emission of volatile organic substances from MCPA-treated soils was lower, with the maximum value of 10.5% being emitted from silt soil. A significant amount of the introduced radioactive material was recovered as residues. The level of 14C-extractable residues for pesticides was low and ranged from 0.9% to 4.9% of total radioactivity. However, the level of 14Cbound residues was significantly greater and ranged from 14.6% to 43.2% of total radioactivity.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79955700550,

title = {Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil},

author = { M. Cycoń and M. Wójcik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955700550&doi=10.1007%2fs10532-010-9430-4&partnerID=40&md5=6b5216e2bfe9bbba157b345700a0039e},

doi = {10.1007/s10532-010-9430-4},

issn = {09239820},

year  = {2011},

date = {2011-01-01},

journal = {Biodegradation},

volume = {22},

number = {3},

pages = {573-583},

abstract = {Degradation of the fungicide thiophanate-methyl (TM) by Enterobacter sp. TDS-1 and Bacillus sp. TDS-2 isolated from sandy soil previously treated with TM was studied in mineral salt medium (MSM) and soil. Both strains were able to grow in MSM supplemented with TM (50 mg l-1) as the sole carbon source. Over a 16 days incubation period, 60 and 77% of the initial dose of TM were degraded by strains TDS-1 and TDS-2, respectively, and disappearance of TM was described by first-order kinetics. Medium supplementation with glucose markedly stimulated bacterial growth; while the final rate of TM degradation was reduced by 21 and 27% for strains TDS-1 and TDS-2, respectively as compared to medium with TM only. Moreover, this additional carbon source changed the TM degradation kinetics, which proceeded according to a zero-order model. This effect was linked to substrate competition and/or a strong decrease of medium pH. Isolates degraded TM (100 mg kg-1) in soil with rate constants of 0.186 and 0.210 day-1, following first-order rate kinetics, and the time in which the initial TM concentration was reduced by 50% (DT50) in soils inoculated with strains TDS-1 and TDS-2 were 6.3 and 5.1 days, respectively. Analysis of TM degradation products in soil showed that the tested strains may have the potential to transform carbendazim (MBC) to 2-aminobenzimidazole (2-AB), and may be useful for a bioremediation of MBC-polluted soils. © 2010 Springer Science+Business Media B.V.},

note = {53},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85027930598,

title = {Significance of silver birch and bushgrass for establishment of microbial heterotrophic community in a metal-mine spoil heap},

author = { S. Sułowicz and T. Płociniczak and Z. Piotrowska-Seget and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027930598&doi=10.1007%2fs11270-010-0417-x&partnerID=40&md5=d0ac7fb2fd48c67f242175ddba961bc0},

doi = {10.1007/s11270-010-0417-x},

issn = {00496979},

year  = {2011},

date = {2011-01-01},

journal = {Water, Air, and Soil Pollution},

volume = {214},

number = {1-4},

pages = {205-218},

publisher = {Kluwer Academic Publishers},

abstract = {Differences in the culturable fractions of total and metal-tolerant bacteria inhabiting bulk soil of a metal-mine spoil heap and the rhizosphere of silver birch (Betula pendula) or bushgrass (Calamagrostis epigejos), completed with changes in total microbial community structure in the soil, were assessed by MIDI-FAME (fatty acid methyl ester) profiling of whole-cell fatty acids. In addition, the abundance of metal-tolerant populations among the culturable bacterial communities and their identity and the metal-tolerance patterns were determined. The high proportions of Cu- and Zn-tolerant bacteria that ranged from 60.6% to 94.8% were ascertained in the heap sites. Within 31 bacterial isolates obtained, 24 strains were Gram-positive and Arthrobacter, Bacillus, Rathayibacter, Brochothrix, and Staphylococcus represented those identified. Minimum inhibitory concentration (MIC) data indicated that several strains developed multi-metal tolerance, and the highest tolerance to Cu (10 mM) and Zn (12 mM) was found for Pseudomonas putida TP3 and three isolated strains (BS3; TP12; and SL16), respectively. The analysis of FAME profiles obtained from the culturable bacterial communities showed that Gram-positive bacteria predominated in bulk soil of all heap sites. In contrast, the rhizosphere communities showed a lower proportion of the Gram-positive group, especially for silver birch. For the total microbial community, mostly Gram-negative bacteria (e.g.; Pseudomonas) inhabited the heap sites. The results suggest that the quantitative and qualitative development of heterotrophic microbiota in the soil of the metal-mine spoil heap seems to be site-dependent (i.e.; rhizosphere vs. bulk soil), according to differences in the site characteristics (e.g.; enrichment of nutrients and total metal concentrations) and impact of plant species. © 2010 Springer Science+Business Media B.V.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84877049803,

title = {Production of polyhydroxyalkanoates from waste frying oil by cupriavidus necator},

author = { R.A.J. Verlinden and D.J. Hill and M.A. Kenward and C.D. Williams and Z. Piotrowska-Seget and I.K. Radecka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84877049803&doi=10.1186%2f2191-0855-1-11&partnerID=40&md5=21770e57d96a9dbc5f81a3d1a94bb1cd},

doi = {10.1186/2191-0855-1-11},

issn = {21910855},

year  = {2011},

date = {2011-01-01},

journal = {AMB Express},

volume = {1},

number = {1},

pages = {1-8},

publisher = {Springer Verlag},

abstract = {Polyhydroxyalkanoates (PHAs) are biopolymers, which can replace petrochemical plastics in many applications. However, these bioplastics are currently far more expensive than petrochemical plastics. Many researchers are investigating the use of inexpensive substrates derived from waste streams. Waste frying oil is abundant and can be used in PHA production without filtration. Cupriavidus necator (formerly known as Ralstonia eutropha) is a versatile organism for the production of PHAs. Small-scale batch fermentation studies have been set up, using different concentrations of pure vegetable oil, heated vegetable oil and waste frying oil. These oils are all rapeseed oils. It has been shown that Cupriavidus necator produced the homopolymer polyhydroxybutyrate (PHB) from the rapeseed oils. The achieved PHB concentration from waste frying oil was 1.2 g/l, which is similar to a concentration that can be obtained from glucose. The PHB harvest from pure oil and heated oil was 0.62 g/l and 0.9 g/l respectively. A feed of waste frying oil could thus achieve more biopolymer than pure vegetable oil. While the use of a waste product is beneficial from a life-cycle perspective, PHB is not the only product that can be made from waste oil. The collection of waste frying oil is becoming more widespread, making waste oil a good alternative to purified oil or glucose for PHB production. © 2011 Verlinden et al; licensee Springer.},

note = {134},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79551713097,

title = {Biodegradation kinetics of 2,4-D by bacterial strains isolated from soil},

author = { M. Cycoń and A. Żmijowska and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79551713097&doi=10.2478%2fs11535-011-0005-0&partnerID=40&md5=683c07191c378adbfd4469899c758bb1},

doi = {10.2478/s11535-011-0005-0},

issn = {1895104X},

year  = {2011},

date = {2011-01-01},

journal = {Central European Journal of Biology},

volume = {6},

number = {2},

pages = {188-198},

publisher = {Versita},

abstract = {The aim of the study was to characterize the 2,4-dichlorophenoxyacetic acid (2;4-D) degradative potential of three bacterial strains identified by MIDI-FAME profiling as Burkholderia cepacia (DS-1), Pseudomonas sp. (DS-2) and Sphingomonas paucimobilis (DS-3) isolated from soil with herbicide treatment history. All strains were capable of using herbicide as the only source of carbon and energy when grown in mineral salt medium (MSM) containing 2,4-D (50 mg/l). Over a 10 day incubation period, 69%, 73% and 54% of the initial dose of 2,4-D were degraded by strains DS-1, DS-2 and DS-3, respectively. Analysis of 2,4-dichlorophenol (2;4-DCP) concentration, the main metabolite of 2,4-D degradation, revealed that strains DS-1 and DS-2 may also have the potential to metabolize this compound. The percentage of 2,4-DCP removal was 67% and 77% in relation to maximum values of 9.5 and 9.2 mg/l determined after 4 and 2 days for MSM+DS-1 and MSM+DS-2, respectively. The degradation kinetics of 2,4-D (50 mg/kg) in sterile soil (SS) showed different potential of tested strains to degrade 2,4-D. The times within which the initial 2,4-D concentration was reduced by 50% (DT 50 ) were 6.3, 5.0 and 9.4 days for SS+DS-1, SS+DS-2 and SS+DS-3, respectively. © 2011 Versita Warsaw and Springer-Verlag Wien.},

note = {29},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79251615424,

title = {Environmental applications of biosurfactants: Recent advances},

author = { M. Pacwa-Płociniczak and G.A. Płaza and Z. Piotrowska-Seget and S.S. Cameotra},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79251615424&doi=10.3390%2fijms12010633&partnerID=40&md5=64c23c77b76235144c093a9cd650bd11},

doi = {10.3390/ijms12010633},

issn = {14220067},

year  = {2011},

date = {2011-01-01},

journal = {International Journal of Molecular Sciences},

volume = {12},

number = {1},

pages = {633-654},

abstract = {Increasing public awareness of environmental pollution influences the search and development of technologies that help in clean up of organic and inorganic contaminants such as hydrocarbons and metals. An alternative and eco-friendly method of remediation technology of environments contaminated with these pollutants is the use of biosurfactants and biosurfactant-producing microorganisms. The diversity of biosurfactants makes them an attractive group of compounds for potential use in a wide variety of industrial and biotechnological applications. The purpose of this review is to provide a comprehensive overview of advances in the applications of biosurfactants and biosurfactant-producing microorganisms in hydrocarbon and metal remediation technologies. © 2010 by the authors; licensee MDPI, Basel, Switzerland.},

note = {588},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-78049346290,

title = {Response of bacteria to heavy metals measured as changes in FAME profiles},

author = { A. Markowicz and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-78049346290&partnerID=40&md5=cb3c70e41d46abdaedb116aeb4340f4c},

issn = {12301485},

year  = {2010},

date = {2010-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {19},

number = {5},

pages = {957-965},

abstract = {The effects of Cd, Ni, Cu, or Zn on the whole cell-derived fatty acid profiles of four bacterial strains isolated from heavy metal-polluted soils located in Upper Silesia was determined. Based on the fatty acid methyl ester (FAME) profiles, the strains were identified and named as Enterobacter intermedius AM15, Enterobacter intermedius MH8b, Pseudomonas putida MH1d, and Klebsiella pneumoniae AM12. The obtained results showed changes that were dependent both on tested strains and metal used. The most significant changes were observed for strains cultured in the Ni presence. In the FAME profiles of MH8b, AM 15, and AM12 strains, a significant increase of cyclopropane fatty acids was observed. Moreover, exposure for Ni resulted in the appearance of a new fatty acid in the FAME profiles of AM15 and MH8b strains. In turn, Cd and Zn caused a decrease of the content of cyclopropane fatty acids as compared to control. For AM15 and AM12 strains cultured on media with heavy metals, the ratio of saturated to unsaturated fatty acids were higher than that in control. The same phenomenon was also observed for MH8b strain exposed only to the highest concentration of Ni and Cd.},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79952003543,

title = {Linuron effects on microbiological characteristics of sandy soils as determined in a pot study},

author = { M. Cycoń and Z. Piotrowska-Seget and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952003543&doi=10.1007%2fs13213-010-0061-0&partnerID=40&md5=56fc288dd81af31a3e8865a933dabc5f},

doi = {10.1007/s13213-010-0061-0},

issn = {15904261},

year  = {2010},

date = {2010-01-01},

journal = {Annals of Microbiology},

volume = {60},

number = {3},

pages = {439-449},

abstract = {The aim of this study was to find what dosages of linuron introduced into soil can be tolerated by microorganisms, and whether a dosage equal to 100-times higher than the predicted environmental concentration (PEC) is harmful. A pot study was performed to determine the effects of the herbicide at PEC (4 mg kg-1), 5 times PEC (20 mg kg-1), and 100 times PEC (400 mg kg-1) on substrate-induced respiration (SIR), four enzyme activities (dehydrogenase; acid and alkaline phosphatases; and urease), ammonification and nitrification rates, plate counts of total bacteria, fungi, N 2-fixing bacteria, nitrifiers and denitrifiers, and distribution of r- and K-strategists in sandy soils of different texture throughout 28 days of incubation. Linuron increased SIR and ammonification, especially at 100 times PEC. In contrast, a decrease in nitrate concentrations was detected in both soils treated with the highest dosage of linuron. Although some changes in microbial numbers were ascertained, they were transient (i.e. total bacteria; N2-fixing bacteria and nitrifiers) or not significant (i.e. total fungi and denitrifiers). Among the enzymes tested, dehydrogenase was the most sensitive to linuron, showing decreased activity for all treatments and two higher dosages in the loamy sand (LS) and sandy loam (SL) soils, respectively. The addition of 100 times PEC of linuron to LS resulted in the domination of slow-growing K-strategists on days 1 and 14. However, r-strategists dominated on both days 1 and 28 in SL. Our results indicate that linuron may disturb indigenous soil microorganisms, especially when released at high concentrations. © Springer-Verlag and the University of Milan 2010.},

note = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77954842425,

title = {Dehydrogenase activity as an indicator of different microbial responses to pesticide-treated soils},

author = { M. Cycoń and Z. Piotrowska-Seget and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954842425&doi=10.1080%2f02757540.2010.495062&partnerID=40&md5=3e2ab4f93b403ae4696960fdf7808fcb},

doi = {10.1080/02757540.2010.495062},

issn = {02757540},

year  = {2010},

date = {2010-01-01},

journal = {Chemistry and Ecology},

volume = {26},

number = {4},

pages = {243-250},

abstract = {A laboratory study was conducted to assess the impacts of diazinon, linuron or a fungicidal preparation of mancozeb supplemented with dimethomorph on dehydrogenase activity (DHA) in loamy sand (LS) and sandy loam (SL) soils with different features. The pesticides were applied at the maximum predicted environmental concentrations (PEC) under field conditions and at 5 or 100 times the PEC. More distinct effects were observed in LS than SL soils at all sampling times. For PEC, a significant decrease in DHA was found in LS soil treated with diazinon or the fungicidal preparation on day 1 and during the incubation period, respectively. However, DHA did not decrease in SL soil treated with the pesticides at this dosage. For the higher concentrations, decreased DHA was ascertained in LS soil treated with diazinon and the fungicidal preparation at all sampling times, whereas for linuron this effect was evident on days 14 and 28. By contrast, only 100-fold PEC significantly decreased DHA in SL soil amended with diazinon or linuron, compared with the fungicidal preparation that decreased DHA at five-fold PEC. Our results indicate that DHA was particularly sensitive to the fungicidal preparation in both soils, whereas linuron was less harmful than diazinon. © 2010 Taylor & Francis.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77954862028,

title = {Responses of indigenous microorganisms to a fungicidal mixture of mancozeb and dimethomorph added to sandy soils},

author = { M. Cycoń and Z. Piotrowska-Seget and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954862028&doi=10.1016%2fj.ibiod.2010.03.006&partnerID=40&md5=b9268784dc5949ffa6ac4451b4c3b374},

doi = {10.1016/j.ibiod.2010.03.006},

issn = {09648305},

year  = {2010},

date = {2010-01-01},

journal = {International Biodeterioration and Biodegradation},

volume = {64},

number = {4},

pages = {316-323},

abstract = {The objective of this study was to assess changes in microbiological characteristics of loamy sand (LS) and sandy loam (SL) soils in response to a mixture of two fungicides, mancozeb (MB) and dimethomorph (DT), applied at three dosages of 15, 75 and 1500 mg kg-1 soil. Microbial activity, assessed by substrate-induced respiration, was either stimulated or unchanged by the first two dosages of MB + DT in both soils throughout 28 days. However, MB + DT at the concentration of 1500 mg kg-1 soil significantly decreased the respiration in LS soil. All MB + DT concentrations increased the total numbers of culturable bacteria, ammonification rate but did not change the numbers of nitrate-reducing bacteria in both soils. In comparison to acid and alkaline phosphatases and urease, dehydrogenase was most sensitive to MB + DT and its activity declined in both soils. Decreases in nitrate concentrations, the numbers of fungi, nitrifying and N2-fixing bacteria were ascertained in both soils during the incubation time. Furthermore, slow growing K-strategists generally dominated in both soils after the treatment with MB + DT. Overall, the fungicidal preparation released at higher concentrations may disturb some non-target microbial groups inhabiting sandy soils. Consequently, to diminish an ecological hazard, careful application of MB + DT should be followed. © 2010 Elsevier Ltd.},

note = {51},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77954682617,

title = {Bioaugmentation as a strategy for cleaning up of soils contaminated with aromatic compounds},

author = { A. Mrozik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954682617&doi=10.1016%2fj.micres.2009.08.001&partnerID=40&md5=3456a6d8ce6f18c596aa4f12acf9e39d},

doi = {10.1016/j.micres.2009.08.001},

issn = {09445013},

year  = {2010},

date = {2010-01-01},

journal = {Microbiological Research},

volume = {165},

number = {5},

pages = {363-375},

abstract = {The contamination of soil with aromatic compounds is of particular environmental concern as they exhibit carcinogenic and mutagenic properties. One of the methods of their removal from soil is bioaugmentation, defined as a technique for improvement of the degradative capacity of contaminated areas by introduction of specific competent strains or consortia of microorganisms. The efficiency of bioaugmentation is determined by many abiotic and biotic factors discussed in this paper. The first include chemical structure, concentration and availability of pollutants as well as physico-chemical properties of soil. In turn, among biotic factors the most important is the selection of proper microorganisms that can not only degrade contaminants but can also successfully compete with indigenous microflora.Several strategies are being developed to make augmentation a successful technology particularly in soils without degrading indigenous microorganisms. These approaches involve the use of genetically engineered microorganisms and gene bioaugmentation. The enhancement of bioaugmentation may be also achieved by delivering suitable microorganisms immobilized on various carriers or use of activated soil. © 2009 Elsevier GmbH.},

note = {330},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77950460464,

title = {Microbial characteristics of sandy soils exposed to diazinon under laboratory conditions},

author = { M. Cycoń and Z. Piotrowska-Seget and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77950460464&doi=10.1007%2fs11274-009-0183-3&partnerID=40&md5=8dd621a9f3977fcf1bfce67545aea6d2},

doi = {10.1007/s11274-009-0183-3},

issn = {09593993},

year  = {2010},

date = {2010-01-01},

journal = {World Journal of Microbiology and Biotechnology},

volume = {26},

number = {3},

pages = {409-418},

abstract = {Changes in microbiological characteristics in response to diazinon, applied at three different dosages of 7, 35 and 700 mg kg-1 soil, were studied in pots filled with sandy soils of different texture. The insecticide dosages corresponded to the maximum predicted environmental concentration (PEC) in field conditions and five or hundred times this rate, respectively. To ascertain these changes, activities of selected soil enzymes, numbers of some microbial populations and bacterial community structure (r/K-strategists) were determined. Acid phosphatase activity and concentrations of ammonium ions either increased or were unaffected by the diazinon dosages. In contrast, nitrate ions, alkaline phosphatase, urease and especially dehydrogenase activities decreased in both soils treated with the higher dosages of the insecticide. The diazinon treatments increased the numbers of culturable bacteria and fungi; however, N-fixing bacteria and nitrifiers (but not denitrifiers) were decreased in both soils. When the high concentration of diazinon (100-times PEC) was added to the loamy sand soil (LS), simulating an undiluted chemical spill, the bacterial community structure shifted towards domination of K-strategists over time. However, r-strategists dominated in the community exposed to the same dosage of the insecticide in the sandy loam soil (SL). Generally, SL soil characteristics were more suitable for microbial activity and growth than those of LS soil were. The results indicate that except a situation of undiluted spill diazinon seems to be a non-hazardous chemical for the culturable soil microorganisms when applied at recommended concentrations. © Springer Science+Business Media B.V. 2009.},

note = {27},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-73149103919,

title = {Changes of FAME profiles as a marker of phenol degradation in different soils inoculated with Pseudomonas sp. CF600},

author = { A. Mrozik and M. Cycoń and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-73149103919&doi=10.1016%2fj.ibiod.2009.11.002&partnerID=40&md5=f46b50be149971890d920c798930b1bf},

doi = {10.1016/j.ibiod.2009.11.002},

issn = {09648305},

year  = {2010},

date = {2010-01-01},

journal = {International Biodeterioration and Biodegradation},

volume = {64},

number = {1},

pages = {86-96},

abstract = {Fatty acid profiles of three different sterile soils contaminated with phenol at the concentrations of 1.7, 3.3 and 5.0 mg g-1 and inoculated with phenol-degrading Pseudomonas sp. CF600 were investigated. The rate of phenol biodegradation and survival of inoculants in contaminated soils were also studied. It was found that introduced strain was able to completely degrade phenol added at the highest dose during 108 days in MF and WM soils, and 124 days in PF soil, whereas at the smallest dose within 60 days independently of soil type. In all phenol-polluted soils the decrease of the initial inoculum was estimated and at the end of the experiment in soils treated with phenol at the concentration of 5.0 mg g-1 constituted 18, 21 and 25% of initial number in MF, PF and WM soils, respectively. Under phenol exposure important changes in profiles of FAMEs obtained directly from soils were detected. The most significant alterations were observed in soils contaminated with the highest phenol dosage and related to 17:0 cy and 19:0 cy ω8c cyclopropane fatty acids. Over time with decreasing phenol concentration the decline of 17:0 cy amount was observed. In turn, in all polluted soils 19:0 cy ω8c appeared first time on day 12 when the initial phenol doses declined about 60% in WM and MF and about 40% in PF soil. Other changes in FAME composition included the slight increase of straight-chain, the decrease of branched, hydroxylated and unsaturated fatty acids. The value of sat/unsat ratio, the occurrence and distribution of specific cyclopropane fatty acids in FAME profiles might be useful for the estimation of phenol biodegradation progress. © 2009 Elsevier Ltd. All rights reserved.},

note = {32},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-67650744553,

title = {Changes in bacterial diversity and community structure following pesticides addition to soil estimated by cultivation technique},

author = { M. Cycoń and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67650744553&doi=10.1007%2fs10646-009-0321-6&partnerID=40&md5=46baeff5689b4ac7708fb8fdfc37d076},

doi = {10.1007/s10646-009-0321-6},

issn = {09639292},

year  = {2009},

date = {2009-01-01},

journal = {Ecotoxicology},

volume = {18},

number = {5},

pages = {632-642},

abstract = {An experiment was conducted under laboratory conditions to investigate the effect of increasing concentrations of fenitrothion (2; 10 and 200 mg a.i./kg soil), diuron (1.5; 7.5 and 150 mg a.i./kg soil) and thiram (3.5; 17.5 and 350 mg a.i./kg soil) on soil respiration, bacterial counts and changes in culturable fraction of soil bacteria. To ascertain these changes, the community structure, bacterial biodiversity and process of colony formation, based on the r/K strategy concept, EP- and CD-indices and the FOR model, respectively, were determined. The results showed that the measured parameters were generally unaffected by the lowest dosages of pesticides, corresponding to the recommended field rates. The highest dosages of fenitrothion and thiram suppressed the peak SIR by 15-70% and 20-80%, respectively, while diuron increased respiration rate by 17-25% during the 28-day experiment. Also, the total numbers of bacteria increased in pesticide-treated soils. However, the reverse effect on day 1 and, in addition, in case of the highest dosages of insecticide on days 14 and 28, was observed. Analysis of the community structure revealed that in all soil treatments bacterial communities were generally dominated by K-strategists. Moreover, differences in the distribution of individual bacteria classes and the gradual domination of bacteria populations belonging to r-strategists during the experiment, as compared to control, was observed. However, on day 1, at the highest pesticide dosages, fast growing bacteria constituted only 1-10% of the total colonies number during 48 h of plate incubation, whereas in remaining samples they reached from 20 to 40% of total cfu. This effect, in case of fenitrothion, lasted till the end of the experiment. At the highest dosages of fenitrothion, diuron and at all dosages of thiram the decrease of biodiversity, as indicated by EP- and CD-indices on day 1, was found. At the next sampling time, no significant retarding or stimulating effect was detected. However, in case of CD values the higher differences were observed. The significant impact of pesticides on the physiological state of soil bacteria was not found. They were generally in dormant state (λ < 0.5), but immediately after pesticides application, the additional reduction of frequency of bacterial cell proliferation (max. decrease of λ value to 0.15 for thiram on day 14) and prolonged retardation time of colony appearance (max. increase of t r value to 1.39 for fenitrothion on day 1) on agar plates were found. © 2009 Springer Science+Business Media, LLC.},

note = {45},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-67349098392,

title = {Biodegradation of the organophosphorus insecticide diazinon by Serratia sp. and Pseudomonas sp. and their use in bioremediation of contaminated soil},

author = { M. Cycoń and M. Wójcik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67349098392&doi=10.1016%2fj.chemosphere.2009.03.023&partnerID=40&md5=ca46d874ac01158dce1eaa65c885e2a7},

doi = {10.1016/j.chemosphere.2009.03.023},

issn = {00456535},

year  = {2009},

date = {2009-01-01},

journal = {Chemosphere},

volume = {76},

number = {4},

pages = {494-501},

publisher = {Elsevier Ltd},

abstract = {An enrichment culture technique was used for the isolation of bacteria responsible for biodegradation of diazinon in soil. Three bacterial strains were screened and identified by MIDI-FAME profiling as Serratia liquefaciens, Serratia marcescens and Pseudomonas sp. All isolates were able to grow in mineral salt medium (MSM) supplemented with diazinon (50 mg L-1) as a sole carbon source, and within 14 d 80-92% of the initial dose of insecticide was degraded by the isolates and their consortium. Degradation of diazinon was accelerated when MSM was supplemented with glucose. However, this process was linked with the decrease of pH values, after glucose utilization. Studies on biodegradation in sterilized soil showed that isolates and their consortium exhibited efficient degradation of insecticide (100 mg kg-1 soil) with a rate constant of 0.032-0.085 d-1, and DT50 for diazinon was ranged from 11.5 d to 24.5 d. In contrast, degradation of insecticide in non-sterilized soil, non-supplemented earlier with diazinon, was characterized by a rate constant of 0.014 d-1 and the 7-d lag phase, during which only 2% of applied dose was degraded. The results suggested a strong correlation between microbial activity and chemical processes during diazinon degradation. Moreover, isolated bacterial strains may have potential for use in bioremediation of diazinon-contaminated soils. © 2009 Elsevier Ltd. All rights reserved.},

note = {203},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-54849417583,

title = {Successive soil treatment with captan or oxytetracycline affects non-target microorganisms},

author = { Z. Piotrowska-Seget and R. Engel and E. Nowak and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-54849417583&doi=10.1007%2fs11274-008-9815-2&partnerID=40&md5=0b10060d5ef148922b36099006868390},

doi = {10.1007/s11274-008-9815-2},

issn = {09593993},

year  = {2008},

date = {2008-01-01},

journal = {World Journal of Microbiology and Biotechnology},

volume = {24},

number = {12},

pages = {2843-2848},

abstract = {Changes in microbial biomass and activity were determined in a sandy-loam soil treated with successive dosages of oxytetracycline (a bactericide) or captan (a fungicide) throughout 98 days of incubation under laboratory conditions. The numbers of culturable bacteria and fungi, total bacterial and fungal biomass (as amounts of phospholipid fatty acids; PLFA), the fungal/bacterial ratio, activities of acid and alkaline phosphatases and urease as well as concentrations of N-NH4+ and N-NO 3- were assessed. Both oxytetracycline and captan significantly decreased numbers of culturable bacteria whereas total bacterial biomass (bactPLFA) was not affected. Oxytetracycline did not effect on the fungal biomass, however their numbers were reduced after the first and second time of soil amendment with the bactericide. Conversely, fungal numbers and biomass (PLFA 18:2ω6;9) significantly decreased in response to soil treatment with the fungicide. Compared to oxytetracycline, captan significantly decreased activities of acid and alkaline phosphatases. For urease activity, the decreased activity was only observed in the soil after the third dosage of captan. Both biocides significantly increased concentrations of N-NH 4+ and decreased concentrations of N-NO3 - after the soil treatments. The results of this study indicate that successive soil treatment with oxytetracycline or captan dosages may negatively affect non-target soil microorganisms and their activities. © 2008 Springer Science+Business Media B.V.},

note = {29},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-50849105095,

title = {FAMEs profiles of phenol-degrading Pseudomonas stutzeri introduced into soil},

author = { A. Mrozik and Z. Piotrowska-Seget and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-50849105095&doi=10.1016%2fj.ibiod.2008.03.009&partnerID=40&md5=a5dea8356d8c54a302d30d94bf98eb5b},

doi = {10.1016/j.ibiod.2008.03.009},

issn = {09648305},

year  = {2008},

date = {2008-01-01},

journal = {International Biodeterioration and Biodegradation},

volume = {62},

number = {3},

pages = {319-324},

abstract = {Fatty acid composition during phenol biodegradation by Pseudomonas stutzeri introduced into three sterile soils differed in organic matter content was investigated. It was found that type of soil strongly influenced the rate of phenol removal and survival of bacteria. In soil (MF) with the highest amount of organic matter (37.30%) the complete phenol biodegradation proceeded within 44 days, in soil (WM) with intermediate organic matter content (18%) lasted 68 days, whereas in sandy soil (PF) (2.10% of organic matter) phenol degradation was not observed. In phenol-polluted soil the number of introduced P. stutzeri cells decreased during experiment period from an initial density of 2.5 × 109 CFU/g soil to 1.73 × 105 CFU/g soil and to 9.7 × 103 CFU/g soil in MF and WM samples, respectively. Under phenol exposure changes in FAMEs profiles isolated from soils were detected. The most significant alterations were connected with the occurrence and content of cyclopropane fatty acids. Fatty acid 17:0 cy was detected only in uncontaminated soil whereas 19:0 cy ω8c fatty acid was synthesised by introduced bacteria in phenol amended soils when its concentration decreased significantly. Other noticeable changes included the increase of branched and straight-chain fatty acid contents, resulted in the higher level of fatty acids saturation in bacteria isolated from phenol-polluted soils. Our results show that fatty acid composition and the occurrence of specific fatty acids can be used for monitoring the progress of bioremediation process in phenol-polluted environment. © 2008.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-58149142977,

title = {Changes in culturable bacterial community of soil treated with high dosages of Cu or Cd},

author = { Z. Piotrowska-Seget and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-58149142977&doi=10.17221%2f428-pse&partnerID=40&md5=bd1f9c3c49f9930044ec9b6ca0e5277c},

doi = {10.17221/428-pse},

issn = {12141178},

year  = {2008},

date = {2008-01-01},

journal = {Plant, Soil and Environment},

volume = {54},

number = {12},

pages = {520-528},

publisher = {Institute of Agricultural and Food Information},

abstract = {Culturable bacteria numbers, kinetics of colony formation, community structure analyses in terms of ecological (r/K-strategists) and cytochemical approaches were used to assess the bacteria responses to soil treatment with high concentrations of Cu or Cd over time. The soil treatment resulted in high concentrations of water-soluble forms of the metals. Bacterial numbers significantly decreased in the metal-treated soil shortly after the soil contamination, their numbers however increased during incubation time. The short- or long-term contact of bacteria with the metals significantly decreased or increased the rate of cell proliferations, respectively. The populations represented by slow-growing K-strategists dominated the structures of bacterial communities with time. The results of fatty acid methyl ester analyses indicated that Gram-positive bacteria dominated shortly after soil contamination; however, changes in the community structures with stimulation of Pseudomonas in the Cd-treated soil occurred after prolonged exposure to the metal. This study shows that short- or long-term exposition to heavy metals may cause different quantitative and qualitative effects on culturable bacteria in soil.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-36048941460,

title = {FAME profiles in Pseudomonas vesicularis during catechol and phenol degradation in the presence of glucose as an additional carbon source},

author = { A. Mrozik and Z. Piotrowska-Seget and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-36048941460&partnerID=40&md5=bc6557d4d2c4888179fd64498ed429e7},

issn = {17331331},

year  = {2007},

date = {2007-01-01},

journal = {Polish Journal of Microbiology},

volume = {56},

number = {3},

pages = {157-164},

abstract = {The aim of this study was to evaluate the impact of catechol and phenol added to culture media separately and with glucose as an additional, easily-degradable carbon source on fatty acid methyl ester (FAME) composition in Pseudomonas vesicularis. Simultaneously, the degradation rates of aromatic substrates used were investigated in single and binary substrate systems. Both catechol and phenol treatments caused changes in the distribution of tested groups of fatty acids. The most noticeable changes included an increase in degree of fatty acid saturation, the appearance of branched and disappearance of hydroxy fatty acids as compared to the control sample with glucose. Under catechol or phenol treatment sat/unsat ratio showed the values of 8.63 and 11.38, respectively, whereas in control cells it reached the value of 2.66. The high level of saturation comes from the high content of cyclopropane fatty acids in bacteria under exposure to aromatic substrates, regardless of the presence of glucose. In these treatments their content was more than 3-fold higher compared to the control. It has been demonstrated that glucose supplementation of culture media containing single aromatic substrate extended the degradation rates of catechol and phenol by P. vesicularis, caused an increase in number of cells but did not significantly change the fatty acid profiles in comparison with bacteria growing on catechol and phenol added to the media individually.},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34547560252,

title = {Effect of selected pesticides on soil microflora involved in organic matter and nitrogen transformations: Pot experiment},

author = { M. Cycoń and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547560252&partnerID=40&md5=23b51131636d16ad34f329dbfb985fee},

issn = {15052249},

year  = {2007},

date = {2007-01-01},

journal = {Polish Journal of Ecology},

volume = {55},

number = {2},

pages = {207-220},

abstract = {The response of soil microflora to increasing concentration of three pesticide preparations containing diazinon (insecticide), linuron (herbicide) and mancozeb + dimethomorph (fungicide) as an active substances, was determined under laboratory conditions. All pesticides were applied at three different dosages, i.e. 7,35 and 700 mg kg-1 soil for the insecticide, 4, 20 i 400 mg kg-1 soil for the herbicide and 15, 75 and 1500 mg kg -1 soil for the fungicide mixture. The lowest dosages corresponded to the maximum predicted environmental concentration (PEC) of pesticides applied in field conditions, while the other dosages were the multiplicities of PEC. After 1, 14 and 28 days of experiment the numbers of heterotrophic bacteria, fungi and bacteria involved in nitrogen transformation (nitrifying; denitrifying and N2-fixing bacteria) was estimated. The numbers of colony forming units (CFU) of respective groups of microorganisms were determined on selective media by means of the serial dilution technique and the spread plate method. The plate-count data indicated that pesticide used affected the numbers of tested microbial groups. All chemicals stimulated the numbers of heterotrophic bacteria and fungi at the higher dosages. However, the reverse effect was detected at the beginning of the experiment. In turn, bacteria involved in nitrogen turnover, particularly N2-fixing and nitrifying bacteria, were the most sensitive to the tested pesticides and their numbers were significantly reduced on each time-point. This great susceptibility of these physiological groups of microorganisms predispose them to be warning biomarkers for the side-effects of environmental pollution caused by chemicals.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34447135626,

title = {Mycorrhizal fungi and ectomycorrhiza associated bacteria isolated from an industrial desert soil protect pine seedlings against Cd(II) impact},

author = { J. Kozdrój and Z. Piotrowska-Seget and P. Krupa},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34447135626&doi=10.1007%2fs10646-007-0149-x&partnerID=40&md5=f81ab4619180da939c196981791429c4},

doi = {10.1007/s10646-007-0149-x},

issn = {09639292},

year  = {2007},

date = {2007-01-01},

journal = {Ecotoxicology},

volume = {16},

number = {6},

pages = {449-456},

abstract = {Effects of mycorrhization with Amanita rubescens or Hebeloma sinapizans and dual inoculation with the fungi and ectomycorrhiza associated bacteria (EMAB) Pseudomonas putida or Bacillus cereus on seedling growth and accumulation of Cd(II) in Pinus sylvestris were studied. Both fungal and bacterial species were isolated from roots of pines growing in an industrial area polluted with high concentrations of heavy metals. During mycorrhization, A. rubescens colonized higher number of pine seedlings than H. sinapizans, especially when EMAB were co-inoculated. In addition, the seedling biometric characteristics (i.e. root and shoot lengths and biomass) were stimulated by treatment with the fungal species alone and dual inoculation with the fungi and EMAB. Amanita rubescens was more efficient in this stimulation than H. sinapizans. The increased growth of pine seedlings was especially seen for co-inoculation with P. putida. Furthermore, elevated accumulation of Cd(II), ranging from 56 μg g -1 to 72 μg g-1 dry weight, in underground parts of the inoculated seedlings was found. The seedlings treated with A. rubescens accumulated higher concentrations of the metal than those inoculated with H. sinapizans. Additional treatment of pine seedlings with P. putida resulted in the higher accumulation of Cd(II) in the roots as compared with those inoculated with B. cereus. The results suggest that the growth of pine seedlings in Cd(II)-polluted soil may depend on fungal species forming ectomycorrhizae, species-specific co-inoculation with EMAB and specificity of fungal-EMAB interactions. © 2007 Springer Science+Business Media, LLC.},

note = {42},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33751085101,

title = {Microbiological characteristics of a sandy loam soil exposed to tebuconazole and λ-cyhalothrin under laboratory conditions},

author = { M. Cycoń and Z. Piotrowska-Seget and A. Kaczyńska and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33751085101&doi=10.1007%2fs10646-006-0099-8&partnerID=40&md5=829385d454f9c84bbff5954a07c1750d},

doi = {10.1007/s10646-006-0099-8},

issn = {09639292},

year  = {2006},

date = {2006-01-01},

journal = {Ecotoxicology},

volume = {15},

number = {8},

pages = {639-646},

abstract = {Changes in microbiological properties of a sandy loam soil in response to the addition of different concentrations of fungicide tebuconazole and pyrethroid insecticide λ-cyhalothrin were assessed under laboratory conditions. To ascertain these changes, the potentially active soil microbial biomass, concentrations of ammonium and nitrate ions, numbers of total culturable bacteria, fungi, nitrogen-fixing bacteria, nitrifying and denitrifying bacteria were determined. Substrate-induced respiration (SIR) increased with time in both control (ranged from 13.7 to 23.7 mg/O 2/kg-1/dry soil/h-1) and pesticide treated soil portions. For both pesticides, SIR values ranged from 12-13 to 23-25 mg/O 2/kg-1/dry soil/h-1 on days 1 and 28, respectively. Also, concentrations of nitrate and ammonium ions, numbers of total culturable bacteria, denitrifying bacteria, nitrogen-fixing bacteria (for the insecticide) and fungi (for the insecticide) were either unaffected or even stimulated by the pesticide treatments. The adverse impacts of the pesticides were observed for nitrate concentrations (on days 1 or 7), numbers of nitrifying bacteria (on day 1), denitrifying bacteria (for the insecticide on days 1 and 14), nitrogen-fixing bacteria (for tebuconazole on day 1) as well as numbers of fungi in tebuconazole-treated soil (on days 1 and 14). © 2006 Springer Science+Business Media, LLC.},

note = {88},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33645663311,

title = {Cellular fatty acid patterns in Pseudomonas sp. CF600 during catechol and phenol degradation in media supplemented with glucose as an additional carbon source},

author = { A. Mrozik and Z. Piotrowska-Seget and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645663311&doi=10.1007%2fBF03174971&partnerID=40&md5=99f9bd1c2c88f39d840d3e1067a42c72},

doi = {10.1007/BF03174971},

issn = {15904261},

year  = {2006},

date = {2006-01-01},

journal = {Annals of Microbiology},

volume = {56},

number = {1},

pages = {57-64},

publisher = {University of Milan},

abstract = {Fatty acid composition in Pseudomonas sp. CF600 during degradation of catechol and phenol individually and their mixture was investigated. Moreover, the influence of glucose as an additional, easily degradable carbon source on fatty acid profiling in bacteria grown on these aromatic substrates was studied. Both catechol and phenol treatments caused in bacterial cells crucial changes in the distribution of tested groups of fatty acids. The major changes included the increase of fatty acid saturation, decrease in the percentage of cyclopropane fatty acid 17:0 cy and the appearance of branched and hydroxy fatty acids. Under catechol, phenol and their mixture exposure saturated/unsaturated ratio showed the value 6.5, 5.68 and 6.38 whereas in control cells this ratio reached the value 3.05. As a response to aromatic compounds bacteria formed fatty acids that were not detected in control cells growing on glucose. It has been demonstrated that the supplementation of cultured media containing single aromatic substrates or/and their mixture with glucose resulted in changes in degradation rates of catechol and phenol. It seemed that glucose influenced some metabolic pathways responsible for the assimilation of aromatic compounds. The incubation of cells in the presence of aromatic compounds and glucose rapidly led to alterations of whole-cell derived fatty acid composition. The most important changes were associated with saturation level of fatty acids and cyclopropane fatty acid contents.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-27944495959,

title = {Bacteria in bioremediation of hydrocarbon-contaminated environments},

author = { A. Mrozik and Z. Piotrowska-Seget and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-27944495959&partnerID=40&md5=c5124fbe740e72f6e5b1b4c92282acb5},

issn = {00794252},

year  = {2005},

date = {2005-01-01},

journal = {Postepy Mikrobiologii},

volume = {44},

number = {3},

pages = {227-238},

abstract = {The application of microorganisms to destroy, or reduce the concentration of, hazardous wastes on contaminated sites is called bioremediation. Such a biological treatment system has various applications, including clean-up of contaminated sites such as water, soils and sludges. A number of bacteria strains existing in natural environment are capable of metabolizing environmental pollutants. Many of them have been utilized in a variety of bioremediation processes. Apart from wild-type strains of bacteria, genetically modified microorganisms (GMMs) have shown potential for bioremediation application. There are many factors that affect the biodegrading processes. Each process depends on species of bacteria, their survival in environment, physico-chemical characteristics of substrate and its availability, and a range of environmental factors, including pH, oxygen concentration, temperature, moisture content, carbon and energy sources. To estimate the effectiveness of bioremediation it is necessary to analyse the diversity of pollutant degrading bacterial populations and detection of catabolic genes in environmental samples. There are many molecular tools (FISH; in situ PCR; DGGE; TGGE; T-RFLP) that are suitable for monitoring changes in bacteria community structure and diversity during hydrocarbon degradation.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-22844448082,

title = {Whole cell-derived fatty acid profiles of Pseudomonas sp. JS150 during naphthalene degradation},

author = { A. Mrozik and S. Łabuzek and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-22844448082&partnerID=40&md5=01ca98386c0bccc35a97820d68af4193},

issn = {17331331},

year  = {2005},

date = {2005-01-01},

journal = {Polish Journal of Microbiology},

volume = {54},

number = {2},

pages = {137-144},

abstract = {Changes in cellular fatty acid composition during naphthalene degradation, at the concentrations of 0.5 g l-1 or 1.0 g l-1, by Pseudomonas sp. JS150 were investigated. In response to naphthalene exposure an increase in saturated/unsaturated ratio was observed. Additionally, the dynamic changes involved alterations in the contents of hydroxy, cyclopropane and branched fatty acids. Among the classes of fatty acids tested the most noticeable changes in the abundance of cyclopropane fatty acids were observed. Since day 4 of incubation these fatty acids were not dectected in bacterial cells growing on naphthalene. In contrast, markedly increased in the percentage of hydroxy fatty acids over time was observed. However, the proportions of saturated straight-chain and branched fatty acids did not change such significantly.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-22344443760,

title = {Relationship between Cognetia sphagnetorum Vejd. (Oligochaeta, Enchytraeidae) and soil microorganisms: A microcosm experiment},

author = { E. Nowak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-22344443760&partnerID=40&md5=c4875cc81814f2c153385049263dd012},

issn = {15052249},

year  = {2005},

date = {2005-01-01},

journal = {Polish Journal of Ecology},

volume = {53},

number = {2},

pages = {215-223},

abstract = {Changes in densities of microorganisms were analysed in Cognetia sphagnetorum Vejd. cultures at normal and low numbers of microorganisms. Different abundance of microorganisms was achieved by using captan and oxytetracycline. During 14 weeks, the following variables were measured: total numbers of bacteria and fungi by plate method, the biomass of microorganisms by PLFA method, content of ions, and activity of acid and alkaline phosphatase and urease. Numbers of enchytraeids increased with numbers of fungi. The presence of animals reduced the activity of soil enzymes and the content of Cl, SO 4, Na, Ca, and Mg ions in soil leacheates. An increase in the content of N-NO3 and N-NH4 was not significant.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-17444403599,

title = {Response of enchytraeid community (Oligochaeta, Enchytraeidae) to manipulation of microbial biomass},

author = { E. Nowak and Z. Piotrowska-Seget and K. Chmielewski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-17444403599&partnerID=40&md5=4aab9dd5851bf239029a2d054cd98ded},

issn = {15052249},

year  = {2005},

date = {2005-01-01},

journal = {Polish Journal of Ecology},

volume = {53},

number = {1},

pages = {53-63},

abstract = {The paper describes a field experiment with the application of two biocides: oxytetracycline that reduced bacteria and captan that reduced soil fungi. The purpose of the experiment was to investigate the response of enchytraeid species to the changes of the abundance and activity of the microbial community as part of their food resources. The following variables were recorded: the biomass of microorganisms by the PLFA method, the rate of cellulose decomposition and the numbers and species composition of enchytraeids. No relationship was found between enchytraeids and abundance of microorganisms, but numbers of animals were correlated with changes in soil cellulolytic activity. Both biocides, even the non toxic oxytetracycline, reduced the diversity (H') of enchytraeid community by increasing the proportion of dominant species. It is suggested that biocides reduced the functional diversity of microorganisms, and this factor had an effect on the enchytraeid populations. The animals responded to the treatment and their community became predominated by the species probably with a broad food spectrum.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-15744373382,

title = {Changes in fatty acid composition in Pseudomonas putida and Pseudomonas stutzeri during naphthalene degradation},

author = { A. Mrozik and S. Łabuzek and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-15744373382&doi=10.1016%2fj.micres.2004.11.001&partnerID=40&md5=46230d6234c55aec86c20ee9a33de12a},

doi = {10.1016/j.micres.2004.11.001},

issn = {09445013},

year  = {2005},

date = {2005-01-01},

journal = {Microbiological Research},

volume = {160},

number = {2},

pages = {149-157},

publisher = {Elsevier GmbH},

abstract = {The effects of naphthalene on the whole cell-derived fatty acid composition of Pseudomonas putida and Pseudomonas stutzeri during naphthalene degradation were investigated. These strains differed in their abilities to degrade naphthalene and in 1,2-catechol dioxygenase activities. The cells of both strains reacted to the addition of naphthalene with an increase in the saturated/unsaturated ratio. The dynamic changes comprised also alterations in the percentage of hydroxy, cyclopropane and branched fatty acids. Upon the exposure of naphthalene, new fatty acids were detected. © 2004 Elsevier GmbH. All rights reserved.},

note = {43},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-12544258607,

title = {Metal-tolerant bacteria occurring in heavily polluted soil and mine spoil},

author = { Z. Piotrowska-Seget and M. Cycoń and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-12544258607&doi=10.1016%2fj.apsoil.2004.08.001&partnerID=40&md5=865bcc5bf1d19f9b3c8126a447bf09ad},

doi = {10.1016/j.apsoil.2004.08.001},

issn = {09291393},

year  = {2005},

date = {2005-01-01},

journal = {Applied Soil Ecology},

volume = {28},

number = {3},

pages = {237-246},

abstract = {The abundance of metal-tolerant populations among culturable bacterial communities in arable soils of different texture and barren spoil of a former silver mine was determined. In addition, bacterial isolates were identified by MIDI-FAME profiling of total-cell fatty acids and plasmid-encoded tolerance to selected heavy metals, as well as the metal-tolerance patterns of the plasmid-containing isolates were ascertained. The difference in the bacterial numbers between the sandy-loam and clay soils was found, which was associated with the effect of higher concentrations of heavy metals, both total and soluble fractions, in the clay soil. The high proportions of metal-tolerant populations in both soils and the spoil were ascertained. In both soils, the bacterial isolates were mostly represented by Gram-negatives, and Variovorax paradoxus dominated in the spoil. Several isolates contained plasmids of the same size, however, only the tolerance to Zn and Cd appeared to be associated with the plasmids, as showed by the transformation data. Minimal inhibitory concentration (MIC) data indicated that the plasmid-containing isolates developed tolerance to several metals tested. Such isolates as Methylobacterium mesopilicum and Flavobacterium from the sandy-loam soil, Pseudomonas gladioli from the clay soil and V. paradoxus from the spoil could be of great interest because of their high tolerance to Zn, Cu, Cd, or Ag, respectively. © 2004 Elsevier B.V. All rights reserved.},

note = {147},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-6444221342,

title = {Cytoplasmatic bacterial membrane responses to environmental perturbations},

author = { A. Mrozik and Z. Piotrowska-Seget and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-6444221342&partnerID=40&md5=e001f8775f0c89f40442aacc64f7c6f4},

issn = {12301485},

year  = {2004},

date = {2004-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {13},

number = {5},

pages = {487-494},

abstract = {Bacteria can adapt to various environmental factors such as temperature, pressure, ions, nutrients and toxic substances by modifying their membranes to maintain them in a fluid state. These modifications within the cytoplasmatic membrane particularly result from changes in the fatty acid composition and interaction between proteins and lipids. Fatty acids, mainly phospholipid fatty acids, play a role as a good biomarker of changes or physiological status of microorganisms caused by external factors. A greater understanding of the detailed physiological mechanisms of bacterial membrane lipid adaptation, especially to toxic substances and solvents, are important for researchers who use bacteria in bioremediation and biotransformation processes.},

note = {48},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-5144234570,

title = {Identification of ectomycorrhizal fungi isolated from roots of birch growing on metallurgic heap},

author = { P. Krupa and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-5144234570&partnerID=40&md5=0ddef5080153836e7e9bc85e541503cf},

issn = {15052249},

year  = {2004},

date = {2004-01-01},

journal = {Polish Journal of Ecology},

volume = {52},

number = {3},

pages = {353-357},

abstract = {Protective role of mycorrhizal fungus for plants against contamination with heavy metals makes a possibility to use fungi inocula for recultivation areas devastated by industry. Use of mycorrhizal symbiont for improving the living conditions of trees requires unambiguous identification of fungi that are able to form successful mycorrhizae in contaminated areas. We took up studies on identification of fungal species, which create mycorrhizal symbiosis with roots of birch growing on metallurgic heap. The identification of fungi collected directly from roots was performed by comparing patterns of restriction fragments of the fungal ITS products with DNA pattern obtained from fruiting bodies of Basidiomycetes fungi growing in the study area.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-2442487892,

title = {Changes in whole cell-derived fatty acids induced by naphthalene in bacteria from genus Pseudomonas},

author = { A. Mrozik and Z. Piotrowska-Seget and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2442487892&doi=10.1016%2fj.micres.2004.02.001&partnerID=40&md5=5c2a5e212e5f9a31ff462c6ed6506164},

doi = {10.1016/j.micres.2004.02.001},

issn = {09445013},

year  = {2004},

date = {2004-01-01},

journal = {Microbiological Research},

volume = {159},

number = {1},

pages = {87-95},

publisher = {Elsevier GmbH},

abstract = {Fatty acid composition during naphthalene utilization was investigated in three strains of bacteria Pseudomonas vesicularis, Pseudomonas stutzeri and Pseudomonas sp. JS150 that expressed different naphthalene degradation abilities. All strains significantly changed their cellular fatty acid profiles as a response to naphthalene exposure. Since naphthalene was present in the medium P. stutzeri increased ratio of saturated/unsaturated fatty acids from 1.1 to 2.1 and Pseudomonas sp. JS150 from 7.5 to 12.0, respectively. In contrast, this ratio decreased from 2.1 to 1.1 in P. vesicularis under the same growth conditions. The changes comprised also alterations in the percentage of selected groups of fatty acids: iso and anteiso, hydroxy and cyclopropane fatty acids. Our results showed that naphthalene induced in tested strains different changes in fatty acids composition. It may suggest that in the presence of naphthalene microorganisms used different adaptive mechanisms to maintain the cells in appropriate physiological state. © 2004 Elsevier GmbH. All rights reserved.},

note = {50},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0344873258,

title = {Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons},

author = { A. Mrozik and Z. Piotrowska-Seget and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0344873258&partnerID=40&md5=51bb676f62d345fccb303d10fc61824a},

issn = {12301485},

year  = {2003},

date = {2003-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {12},

number = {1},

pages = {15-25},

abstract = {Many polycyclic aromatic hydrocarbons (PAHs) are known to be toxic and carcinogenic for humans, and their contamination of soils and aquifers is of great environmental concern. Some microorganisms, mainly from the genera Pseudomonas and Mycobacterium, were found to be capable of transforming and degrading PAHs. These abilities may be useful in removal of PAHs from the environment. The successful application of bacteria to the bioremediation of PAH-contaminated sites requires a deeper understanding of how microbial PAH degradation proceeds. In this review, the bacteria involved and the metabolic pathways for the degradation of many PAHs are summarized and the biological aspects of PAH bioremediation are discussed.},

note = {169},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0942280438,

title = {Positive aspects of interaction between plants and mycorrhizal fungi originating from soils polluted with cadmium},

author = { P. Krupa and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0942280438&partnerID=40&md5=0b10919175a35a975a49b53566a0bf6d},

issn = {12301485},

year  = {2003},

date = {2003-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {12},

number = {6},

pages = {723-726},

abstract = {A laboratory study was carried out to evaluate the protective role of ectomycorrhizal fungi against contamination of plants growing in soil treated with cadmium at a dose of 150 μg Cd/ g soil. An alginate-immobilized inoculum of mycorrhizal fungi was used to introduce the fungi to the soil. The impact of fungi was examined in terms of changes in cadmium levels in inoculated and non-inoculated seedlings of Pinus sylvestris L. It was found that the concentration of cadmium in plants inoculated with fungi was significantly lower than in non-inoculated seedlings. We also observed that the total concentration of cadmium in contaminated soil inoculated with fungi was lower than in non-inoculated soil.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0942269623,

title = {Signature Lipid Biomarker (SLB) analysis in determining changes in community structure of soil microorganisms},

author = { Z. Piotrowska-Seget and A. Mrozik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0942269623&partnerID=40&md5=eb0deaaeb27928c47fb219f5bd2ad017},

issn = {12301485},

year  = {2003},

date = {2003-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {12},

number = {6},

pages = {669-675},

abstract = {Signature lipid biomarker (SLB) analysis is a useful tool for identifying microorganisms and characterizing microbial communities in natural systems. Specific fatty acids, especially phospholipids (PLFA), are essential membrane components, make up a relatively constant proportion of the microorganisms under natural conditions and their patterns provide insight into the bacterial and fungal community structure and biomass. This method is based on direct extraction of fatty acids from cultured bacteria or environmental samples and determining the isolated methyl ester fatty acids (FAME) using gas chromatography (GC). Several PLFAs are useful markers for the detection of the specific groups, and whole cell fatty acid analysis is used for routine identification of microbial species. The fatty acid analysis has been successfully applied for the characterization of microbial communities from agricultural soils, from sites contaminated with heavy metals, aromatic compounds, alkaline dust, acid rain and from other diverse habitats.},

note = {47},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0036066821,

title = {Functional characterization of Gne (UDP-N-acetylglucosamine-4-epimerase), Wzz (chain length determinant), and Wzy (O-antigen polymerase) of Yersinia enterocolitica serotype O:8},

author = { J.A. Bengoechea and E. Pinta and T. Salminen and C. Oertelt and O. Holst and J. Radziejewska-Lebrecht and Z. Piotrowska-Seget and R. Venho and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036066821&doi=10.1128%2fJB.184.15.4277-4287.2002&partnerID=40&md5=afe8c7f264502b9ce3e654f2bde40758},

doi = {10.1128/JB.184.15.4277-4287.2002},

issn = {00219193},

year  = {2002},

date = {2002-01-01},

journal = {Journal of Bacteriology},

volume = {184},

number = {15},

pages = {4277-4287},

abstract = {The lipopolysaccharide (LPS) O-antigen of Yersinia enterocolitica serotype O:8 is formed by branched pentasaccharide repeat units that contain N-acetylgalactosamine (GalNAc), L-fucose (Fuc), D-galactose (Gal), D-mannose (Man), and 6-deoxy-D-gulose (6d-Gul). Its biosynthesis requires at least enzymes for the synthesis of each nucleoside diphosphate-activated sugar precursor; five glycosyltransferases, one for each sugar residue; a flippase (Wzx); and an O-antigen polymerase (Wzy). As this LPS shows a characteristic preferred O-antigen chain length, the presence of a chain length determinant protein (Wzz) is also expected. By targeted mutagenesis, we identify within the O-antigen gene cluster the genes encoding Wzy and Wzz. We also present genetic and biochemical evidence showing that the gene previously called galE encodes a UDP-N-acetylglucosamine-4-epimerase (EC 5.1.3.7) required for the biosynthesis of the first sugar of the O-unit. Accordingly, the gene was renamed gne. Gne also has some UDP-glucose-4-epimerase (EC 5.1.3.2) activity, as it restores the core production of an Escherichia coli K-12 galE mutant. The three-dimensional structure of Gne was modeled based on the crystal structure of E. coli GalE. Detailed structural comparison of the active sites of Gne and GalE revealed that additional space is required to accommodate the N-acetyl group in Gne and that this space is occupied by two Tyr residues in GalE whereas the corresponding residues present in Gne are Leu136 and Cys297. The Gne Leu136Tyr and Cys297Tyr variants completely lost the UDP-N-acetylglucosamine-4-epimerase activity while retaining the ability to complement the LPS phenotype of the E. coli galE mutant. Finally, we report that Yersinia Wzx has relaxed specificity for the translocated oligosaccharide, contrary to Wzy, which is strictly specific for the O-unit to be polymerized.},

note = {92},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0036065922,

title = {Fatty acids of bacterial membranes as a biomarker of aromatic compounds toxicity [Kwasy tłuszczowe błon komórkowych bakterii jako wskaźniki toksyczności zwia̧zków aromatycznych]},

author = { A. Mrozik and Z. Piotrowska-Seget and S. Łabuzek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036065922&partnerID=40&md5=2b67095739e1a42361b146440a8bbebc},

issn = {00794252},

year  = {2002},

date = {2002-01-01},

journal = {Postepy Mikrobiologii},

volume = {41},

number = {2},

pages = {185-197},

abstract = {In all bacteria fatty acids are found as a major component of their membrane lipids. The well-known spectrum of bacterial fatty acids comprises saturated, cis, trans-monounsaturated, diunsaturated, cyclopropane, hydroxyl and methyl-branched fatty acids. Recently a new type has been found as membrane constituents - polyunsaturated fatty acids. The physical properties of bacterial membranes are determined by the composition of fatty acids. Some membrane active agents, including aromatic compounds, have a strong influence on membrane fluidity. These compounds dissolve in the cell membrane disturbing its integrity and affecting specific permeabilization. The hydrophobicity of an aromatic compound, expressed as its logP value, is a good indicator of its toxicity. Microorganisms however can adapt to many organic compounds by changing of their membrane fluidity. They can modify degree of saturation of fatty acids, the average chain length and the protein content. This mechanism is called "homeoviscosic adaptation". One of the key processes in the adaptation of some Pseudomonas, Vibrio and Escherichia coli strains, enabling them to tolerate aromatic compounds, appears to be the isomerization of cis - into trans-unsaturated fatty acids. The analysis of microbial membrane lipids, specifically phospholipid fatty acids (PLFA) is a powerful tool for monitoring the microbial responses to changes in their environment. Phospholipids are extracted directly from environmental samples to characterise microorganisms within their communities. Microbial PLFA analysis provides quantitative insight into three important attributes of microbial communities, viable biomass, community structure and metabolic activity. Certain pollutants induce changes in some PLFA components such as ratio of saturated to unsaturated fatty acids, ratio of trans to cis-monoenoic unsaturated fatty acids, and the content of cyclopropane fatty acids. It might function as an indicator for the toxicity of many aromatic pollutants, particularly during in situ bioremediation and biotransformation processes.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0033043394,

title = {Indigenous microflora responses to introduction of cyanogenic strains of Pseudomonas fluorescens into soil},

author = { Z. Piotrowska-Seget and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033043394&partnerID=40&md5=0339b0e75f89e41ce720254dbd869a4a},

issn = {01371320},

year  = {1999},

date = {1999-01-01},

journal = {Acta Microbiologica Polonica},

volume = {48},

number = {1},

pages = {73-78},

abstract = {The effects of cyanogenic Pseudomonas fluorescens strains introduced into soil on the kinetic of colony formation and bacterial community structure were investigated. About 7.8 x l08 and 1.2 x 109 cfu per g dry soil of TA1 and B2 were added to the soil portions, respectively. The parameters of colony formation by heterotrophic soil bacteria were determined. The bacterial community structure and phenotypic diversity were studied using concept of r/K strategies and ecophysiological index, respectively. The physiological state of indigenous heterotrophic bacteria and gram-negative group did not change under the influence of the cyanogenic strains introduced. Phenotypic diversity of the soil bacteria also did not change significantly. However, some short-term shifts in community structure of indigenous heterotrophic bacteria were noticed. This study shows that the introduction of great numbers of cyanogenic P. fluorescens strains could be safely used as potential agents in biological control of soil-born pathogens.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0344526894,

title = {Effects of introduced pseudomonads and soil treatment with copper on physiological state of indigenous microflora},

author = { J. Kozdrój and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0344526894&partnerID=40&md5=37df8454ebde28adc2c6672cdd8f5df4},

issn = {01371320},

year  = {1996},

date = {1996-01-01},

journal = {Acta Microbiologica Polonica},

volume = {45},

number = {1},

pages = {85-91},

abstract = {The effects of either Pseudomonas fluorescens strains introduced or soil contamination with different doses of copper on the kinetic of colony formation by indigenous bacteria in the rhizosphere of bean (Phaseolus vulgaris L.) and in the non-rhizosphere soil were investigated. The indigenous heterotrophic bacteria were represented by slow growing populations in quiescent state that did not change under the influence of added either pseudomonads or different doses of copper. The indigenous gram-negative bacteria were also in quiescent state. The introduction of pseudomonads, however, caused transient appearance of proliferating populations (in vegetative state) in the non-rhizosphere soil. The addition of copper at different doses prolonged the retardation of colony appearance time, especially noticeable in the non-rhizosphere soil. No interaction effect of the introduced pseudomonads and soil treatment with different doses of copper on physiological state of indigenous heterotrophic and gram-negative bacteria was found.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0037961343,

title = {Survival of the cyanogenic strains of Pseudomonas fluorescens and their effect on indigenous microflora in the rhizosphere of bean (Phaseolus vulgaris L.)},

author = { Z. Piotrowska-Seget and K. Sroka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037961343&partnerID=40&md5=b8a5aa57e182a552b723b33be713daed},

issn = {01371320},

year  = {1996},

date = {1996-01-01},

journal = {Acta Microbiologica Polonica},

volume = {45},

number = {1},

pages = {93-101},

abstract = {A laboratory studies were carried out to evaluate survival of strains of Pseudomonas fluorescens TA1 and B2 introduced to the rhizosphere of bean and their impact on indigenous microflora. The both strains survived in the rhizosphere over a period of 28 days, but their number decreased rapidly, especially in first 14 days of the experiment. The introduced strains of Ps. fluorescens did not cause significant changes in the total number of heterotrophic, pseudomonads and gram-negative bacteria. The data suggest that the introduced cyanogenic pseudomonads did not change stability of indigenous microflora.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0029584702,

title = {Indigenous Microflora And Bean Responses To Introduction Of Genetically Modified Pseudomonas Fluorescens Strains Into Soil Contaminated With Copper},

author = { J. Kozdrój and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029584702&doi=10.1080%2f10934529509376329&partnerID=40&md5=c9bf1854bc447cf32d9a6cc699062c40},

doi = {10.1080/10934529509376329},

issn = {10771204},

year  = {1995},

date = {1995-01-01},

journal = {Journal of Environmental Science and Health. Part A: Environmental Science and Engineering and Toxicology},

volume = {30},

number = {10},

pages = {2133-2158},

abstract = {A laboratory study was carried out to evaluate survival of genetically altered strains of Pseudomonas fiuorescens in the rhizosphere of bean and in bulk soil, and the effect on indigenous microflora. The strains were released into uncontaminated soil and soil contaminated with copper. Numbers of plasmid RP4 donor, recipient and transconjugant cells were determined after 10 and 28 days. The impact on indigenous microflora was examined in terms of changes in total numbers of heterotrophic and Gram-negative bacteria, and shifts in microbial community structure. Microbial community structures were determined using a method based on the r- and K-strategists concept. The reaction of bean seedlings to the introduced strains in uncontaminated soil and soil contaminated with copper was also studied. Higher copper dose (2.5 mg g'1) markedly decreased the numbers of donor and recipient cells while transconjugant cells were not found at all. The donor carrying plasmid RP4 survived better than the recipient in the rhizosphere and bulk soil. The introduced strains of P. fluorescens did not cause significant changes in the numbers of indigenous heterotrophic and Gram-negative bacteria in the rhizosphere and bulk soil. The numbers of these bacteria increased in response to soil contamination with copper. In the rhizosphere, the introduced strains of P. fluorescens did not affect the structure of indigenous heterotrophic and Gram-negative bacteria belonging to slow growing K-strategists. Soil contamination with copper decreased the growth of indigenous microflora. In the bulk soil, short-term shifts towards fast growing r-strategists was observed. However, K-strategists dominated in soil contaminated with copper, especially in samples also inoculated with P. fluorescens strains. The introduction of P. fluorescens strains into the soil did not affect phenotypic diversity (eco-physiological index) of indigenous microflora in the rhizosphere and bulk soil. Changes in bean shoots and roots were observed after 28 days. The data suggest that the genetically altered pseudomonads introduced did not affect the stability of indigenous microflora in the rhizosphere and bulk soil, nor plant growth. © 1995, Taylor & Francis Group, LLC. All rights reserved.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}