@article{2-s2.0-85211618567,

title = {The paradoxical effects of beneficial bacteria on Solanum lycopersicum under Cd stress},

author = { M. Noszczyńska and M. Pawlik and M. Rudnicka and D. Wójcik and M. Gajecka and K. Kukucz and M. Skowronek and I.W. Potocka and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85211618567&doi=10.1016%2fj.envpol.2024.125430&partnerID=40&md5=4ce49372be49e0ed95cdb86bd4376fe1},

doi = {10.1016/j.envpol.2024.125430},

year  = {2025},

date = {2025-01-01},

journal = {Environmental Pollution},

volume = {366},

publisher = {Elsevier Ltd},

abstract = {This study investigated the complex interactions between a novel consortium and tomato seedlings under cadmium (Cd) stress. The consortium consists of two bacteria, Pseudomonas sp. HS4 and Paenarthrobacter sp. AS8, both with proven plant growth-promoting (PGP) properties, isolated from Cd hyperaccumulators. Our research highlights the paradoxical effects of these bacteria, revealing their dual role in reducing Cd uptake while simultaneously inducing oxidative stress in plants. Hydroponic experiments showed that the consortium reduced Cd accumulation in tomato shoots by 52% compared to uninoculated controls. However, this reduction was accompanied by decreased plant biomass and increased oxidative stress, with malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) levels up to 80% and 160% higher, respectively, in inoculated plants. Root H₂O₂ production increased by 38% under 50 μM Cd without a corresponding rise in catalase (CAT) activity. Despite Cd exposure, the consortium promoted chlorophyll and carotenoid synthesis, restoring pigment levels to those of unstressed controls. Gene expression analysis revealed a complex impact on stress responses, with inoculation suppressing Sl1 gene expression in roots and upregulating the oxidative stress-related GR-1 gene in shoots. These findings highlight the complex and multifaceted relationship between beneficial bacteria and plant fitness under heavy metal stress, with significant implications for sustainable agriculture. The study raises new questions regarding the broader physiological and ecological impacts of applying hyperaccumulator-associated bacteria in crop management, emphasizing the necessity for deeper mechanistic insights into these interactions to fully harness their potential in improving crop resilience and productivity. © 2024 Elsevier Ltd},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85206536704,

title = {The Influence of Activated Sludge Augmentation on Its Ability to Degrade Paracetamol},

author = { A. Dzionek and D. Wojcieszyńska and O.A. Menashe and D. Szada and I.W. Potocka and T. Jesionowski and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85206536704&doi=10.3390%2fmolecules29194520&partnerID=40&md5=8105f0323057435af5bfe503e5099f48},

doi = {10.3390/molecules29194520},

year  = {2024},

date = {2024-01-01},

journal = {Molecules},

volume = {29},

number = {19},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {Paracetamol is one of the most commonly used painkillers. Its significant production and consumption result in its presence in the environment. For that reason, paracetamol has a negative impact on the organisms living in ecosystems. Therefore, it is necessary to develop effective methods to remove paracetamol from sewage. One of the methods is the bioaugmentation of activated sludge with organisms with increased degradation potential in relation to paracetamol. This study determined the effectiveness of paracetamol degradation by activated sludge augmented with a free or immobilised Pseudomonas moorei KB4. To immobilise the strain, innovative capsules made of cellulose acetate were used, the structure of which provides an optimal environment for the development of bacteria. Augmentation with both a free and immobilised strain significantly improves the efficiency of paracetamol biodegradation by activated sludge. Over a period of 30 days, examined systems allowed ten doses of paracetamol decomposition, while the unaugmented system degraded only four. At the same time, using the immobilised strain does not significantly affect the functioning of the activated sludge, which was reflected in the stability of processes such as nitrification. Due to the high stability of the preparation, it can become a valuable tool in wastewater treatment processes. © 2024 by the authors.},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85184913863,

title = {Decomposition of non-steroidal anti-inflammatory drugs by activated sludge supported by biopreparation in sequencing batch reactor},

author = { A. Dzionek and A. Nowak and D. Wojcieszyńska and I.W. Potocka and W. Smułek and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184913863&doi=10.1016%2fj.biortech.2024.130328&partnerID=40&md5=39d790eda0f9fbd9643a4146cbc5c18d},

doi = {10.1016/j.biortech.2024.130328},

year  = {2024},

date = {2024-01-01},

journal = {Bioresource Technology},

volume = {395},

publisher = {Elsevier Ltd},

abstract = {The presence of non-steroidal anti-inflammatory drugs in wastewater from sewage treatment plants indicates that they are not completely biodegradable. The designed biopreparation based on immobilized bacteria enables the degradation of paracetamol, ibuprofen, naproxen and diclofenac at a rate of 0.50 mg/L*day, 0.14 mg/L*day, 0.16 mg/L*day and 0.04 mg/L*day, respectively. Lower degradation of drugs in the mixture than in monosubstrate systems indicates their additive, antagonistic effect, limiting the degradative capacity of microorganisms. The biopreparation is stable for at least 6 weeks in bioreactor conditions. Biochemical parameters of activated sludge functioning showed increased oxygen demand, which was related to increased ammonia concentration caused by long-term exposure of activated sludge to drugs. Reduced metabolic activity was also observed. The preparation enables decomposing drugs and their metabolites, restoring the activated sludge's functionality. The tested biopreparation can support activated sludge in sewage treatment plants in degrading non-steroidal anti-inflammatory drugs and phenolic compounds. © 2024 Elsevier Ltd},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85181777064,

title = {Naproxen as environmental pollution, its effect on bacteria metabolism and degradation mechanism in immobilized Planococcus sp. S5},

author = { A. Dzionek and D. Wojcieszyńska and A. Marchlewicz and W. Smułek and I.W. Potocka and Ł. Jałowiecki and J. Borgulat and G.A. Płaza and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85181777064&doi=10.1016%2fj.cej.2023.148174&partnerID=40&md5=899216654a60c6867293bebad6864093},

doi = {10.1016/j.cej.2023.148174},

year  = {2024},

date = {2024-01-01},

journal = {Chemical Engineering Journal},

volume = {481},

publisher = {Elsevier B.V.},

abstract = {Planococcus sp. S5 belongs to strains that degrade naproxen, one of the most popular non-steroidal anti-inflammatory drugs. In the presented work, the immobilization of the S5 strain was carried out on the Loofah plant sponge, which improved the degradation efficiency, and kinetic studies indicated the abolition of the inhibition by the substrate observed in the free cell system. At the same time, after immobilization, evident changes were observed in the metabolic profile of the strain, which was related to the specific microenvironment of the carrier. The study also presents the naproxen degradation pathway in a system with the immobilized S5 strain for the first time. The analysis of intermediates formed during the decomposition of naproxen indicated that this decomposition occurs through naphthalene and salicylic acid. Furthermore, the degradation of naproxen via 3-hydroxybenzoic acid to gentisic acid is also possible. The high efficiency of naproxen degradation by the immobilised S5 strain enables its use in bioremediation. © 2023 Elsevier B.V.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85196705494,

title = {Sensitivity and strength of maize roots facing different physical conditions of the growth medium},

author = { J. Śróbka and I.W. Potocka and J. Karczewski and J. Szymanowska-Pułka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85196705494&doi=10.5586%2fasbp%2f187377&partnerID=40&md5=940a7389bec9c8b459a0e6494040c21c},

doi = {10.5586/asbp/187377},

year  = {2024},

date = {2024-01-01},

journal = {Acta Societatis Botanicorum Poloniae},

volume = {93},

publisher = {Polish Botanical Society},

abstract = {The morphology of a plant’s root is strongly affected by the compaction of the growth medium, the size of its particles, or the presence of non-movable obstacles. However, little is known about the effect of these characteristics on root anatomy and mechanical properties of the root tissues. Anatomical features of maize roots grown in media that varied in density and/or structure (soil; glass beads; vermiculite) were analyzed on cross-sections through the elongation and maturation zones of the roots of 14-day-old seedlings. The sections were stained for lignin and suberin to recognize the developmental stages of exodermis and endodermis. Cortex thickness, number of cortical cell layers, and diameter of the vascular cylinder (stele) were measured in both zones. The Young’s modulus of the roots was determined using mechanical tensile tests. Assuming that the root can be considered a composite material, a model was used that allowed, for the first time, the estimation of the mechanical properties of the stele and cortex. While the cell arrangement of roots grown in a medium with high density and fine movable particles (soil) was regular, roots grown in a medium with low density and light particles (vermiculite) and a medium with high density and large unmovable particles (glass beads) showed early damage of the rhizodermis and impaired cell arrangement in the cortex and vascular cylinder. In these roots, the exodermis and endodermis matured closer to the root tip than in roots from the soil. The vermiculite roots were the most outliers in terms of morphometric parameters and mechanical properties. The Young’s modulus of the stele was many times greater than the Young’s modulus of the cortex in the roots of all variants. Of the media used in the experiment, the soil appears to be most favorable for the maize root growth and development. © The Author(s) 2024.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85152414271,

title = {Native Bacteria Isolated from Phosphate Deposits Reveal Efficient Metal Biosorption and Adhesion to Ore Particles},

author = { H. Rabia and M. Ould Hamou and K. Kasperkiewicz and T. Krzykawski and M. Malicka and I.W. Potocka and I. Bodnaruk and A. Merchichi and M. Skowronek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85152414271&doi=10.3390%2fmin13030388&partnerID=40&md5=1bfaf6d1a995b0e8847e053403d2f345},

doi = {10.3390/min13030388},

issn = {2075163X},

year  = {2023},

date = {2023-01-01},

journal = {Minerals},

volume = {13},

number = {3},

publisher = {MDPI},

abstract = {Mining and processing phosphate ore are among the essential branches of the economy in some developing countries, including Algeria. Conventional ore beneficiation methods can harm the environment by consuming tremendous amounts of water resources (during washing and flotation), potentially hazardous chemicals, and thermal energy. Mine water contains toxic metals that, when released, interfere with environmental functioning. Therefore, in line with environmental needs, conventional methods should be gradually replaced with safe biotechnological processes. This study aimed to investigate the biosorption and adhesion abilities of native microorganisms isolated from Djebel Onk ore (Algeria). The examined bacterial strains differed in their metal accumulation efficiency. The incubation of phosphate ore with the native strain Bacillus HK4 significantly increased the recovery of Mg and Cd (at pH 7; 8147.00 and 100.89 µg/g−1; respectively). The HK4 strain also revealed better adhesion to the ore particles than the reference strain of Bacillus subtilis. Thus, biosorption could be more effective when using the native HK4 strain, which can remove Cd and/or Mg over a pH 4–10 range. Moreover, concerning the unique adhesion capacity of HK4, the strain can be considered in the design of bioflotation methods, as well as in the development of an eco-friendly method of ore and post-flotation waste beneficiation. © 2023 by the authors.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85138690790,

title = {Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation},

author = { D. Wojcieszyńska and J. Klamka and A. Marchlewicz and I.W. Potocka and J. Żur and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138690790&doi=10.3390%2fmolecules27185795&partnerID=40&md5=2dda4f9763acf86cf0ed785d52ea3576},

doi = {10.3390/molecules27185795},

issn = {14203049},

year  = {2022},

date = {2022-01-01},

journal = {Molecules},

volume = {27},

number = {18},

publisher = {MDPI},

abstract = {Immobilization is a commonly used method in response to the need to increase the resistance of microorganisms to the toxic effects of xenobiotics. In this study, a plant sponge from Luffa cylindrica was used as a carrier for the immobilization of the Stenotrophomonas maltophilia KB2 strain since such a carrier meets the criteria for high-quality carriers, i.e., low price and biodegradability. The optimal immobilization conditions were established as a temperature of 30 °C, pH 7.2, incubation time of 72 h, and an optical density of the culture of 1.4. The strain immobilized in such conditions was used for the biodegradation of naproxen, and an average rate of degradation of 3.8 µg/hour was obtained under cometabolic conditions with glucose. The obtained results indicate that a microbiological preparation based on immobilized cells on a luffa sponge can be used in bioremediation processes where it is necessary to remove the introduced carrier. © 2022 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85123106657,

title = {IMSI—Guidelines for Sperm Quality Assessment},

author = { K. Lukaszuk and G. Jakiel and I.W. Potocka and J. Kiewisz and J. Olszewska and W. Sieg and A. Podolak and E. Pastuszek and A. Wdowiak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123106657&doi=10.3390%2fdiagnostics12010192&partnerID=40&md5=efdbf84758d6a94f3ca0c95f5b7907ca},

doi = {10.3390/diagnostics12010192},

issn = {20754418},

year  = {2022},

date = {2022-01-01},

journal = {Diagnostics},

volume = {12},

number = {1},

publisher = {MDPI},

abstract = {Intracytoplasmic sperm injection (ICSI) is a widely used and accepted treatment of choice for oocyte fertilization. However, the quality of sperm selection depends on the accurate visualization of the morphology, which can be achieved with a high image resolution. We aim to correct the conviction, shown in a myriad of publications, that an ultra-high magnification in the range of 6000×–10,000× can be achieved with an optical microscope. The goal of observing sperm under the microscope is not to simply get a larger image, but rather to obtain more detail—therefore, we indicate that the optical system’s resolution is what should be primarily considered. We provide specific microscope system setup recommendations sufficient for most clinical cases that are based on our experience showing that the optical resolution of 0.5 µm allows appropriate visualization of sperm defects. Last but not least, we suggest that mixed research results regarding the clinical value of IMSI, comparing to ICSI, can stem from a lack of standardization of microscopy techniques used for both ICSI and IMSI. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85119590030,

title = {The pellicle–Another strategy of the root apex protection against mechanical stress?},

author = { I.W. Potocka and J. Szymanowska-Pułka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119590030&doi=10.3390%2fijms222312711&partnerID=40&md5=f856fde8ce040b59f5d0d7d956a82e1b},

doi = {10.3390/ijms222312711},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {23},

publisher = {MDPI},

abstract = {In grasses, the apical part of the root is covered by a two-layered deposit of extracellular material, the pellicle, which together with the outer periclinal wall of protodermal cells forms the three-layered epidermal surface. In this study, the effect of mechanical stress on the pellicle was examined. An experiment was performed, in which maize roots were grown in narrow diameter plastic tubes with conical endings for 24 h. Two groups of experimental roots were included in the analysis: stressed (S) roots, whose tips did not grow out of the tubes, and recovering (R) roots, whose apices grew out of the tube. Control (C) roots grew freely between the layers of moist filter paper. Scanning electron microscopy and confocal microscopy analysis revealed microdamage in all the layers of the epidermal surface of S roots, however, protodermal cells in the meristematic zone remained viable. The outermost pellicle layer was twice as thick as in C roots. In R roots, large areas of dead cells were observed between the meristematic zone and the transition zone. The pellicle was defective with a discontinuous and irregular outermost layer. In the meristematic zone the pellicle was undamaged and the protodermal cells were intact. The results lead to the conclusion that the pellicle may prevent damage to protodermal cells, thus protecting the root apical meristem from the negative effects of mechano-stress. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85090585955,

title = {Xanthan gum as a carrier for bacterial cell entrapment: Developing a novel immobilised biocatalyst},

author = { A. Dzionek and D. Wojcieszyńska and M. Adamczyk-Habrajska and J. Karczewski and I.W. Potocka and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090585955&doi=10.1016%2fj.msec.2020.111474&partnerID=40&md5=4a58bcdb7fc091b5c8bf180f466e858d},

doi = {10.1016/j.msec.2020.111474},

issn = {09284931},

year  = {2021},

date = {2021-01-01},

journal = {Materials Science and Engineering C},

volume = {118},

publisher = {Elsevier Ltd},

abstract = {Xanthan gum (XAN) is a widely used polysaccharide in various industries. Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen. The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%. A negative impact of entrapment was noted for strain Planococcus sp. S5, which showed a survival rate in the 93–51% range. To achieve good mechanical properties of the composites, they were additionally hardened using polydopamine (PDA). XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules. Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98–99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive. This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora. Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications. © 2020 Elsevier B.V.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85091670721,

title = {Tocopherols mutual balance is a key player for maintaining Arabidopsis thaliana growth under salt stress},

author = { E. Surówka and I.W. Potocka and M.A. Dziurka and J. Wróbel-Marek and E.U. Kurczyńska and I. Żur and A. Maksymowicz and E. Gajewska and Z. Miszalski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091670721&doi=10.1016%2fj.plaphy.2020.09.008&partnerID=40&md5=178d47e55217a42bf706da8ce75b97d7},

doi = {10.1016/j.plaphy.2020.09.008},

issn = {09819428},

year  = {2020},

date = {2020-01-01},

journal = {Plant Physiology and Biochemistry},

volume = {156},

pages = {369-383},

publisher = {Elsevier Masson s.r.l.},

abstract = {Enhanced channeling carbon through pathways: shikimate/chorismate, benzenoid-phenylopropanoid or 2-C-methyl-D-erythritol 4-phosphate (MEP) provides a multitude of secondary metabolites and cell wall components and allows plants response to environmental stresses. Through the biosynthetic pathways, different secondary metabolites, like tocopherols (TCs), are bind to mutual dependencies and metabolic loops, that are not yet fully understood. We compared, in parallel, the influence of α- and γ-TCs on metabolites involved in osmoprotective/antioxidative response, and physico-chemical modification of plasma membrane and cell wall. We studied Arabidopsis thaliana Columbia ecotype (WT), mutant vte1 deficient in α- and γ-TCs, mutant vte4 over-accumulating γ-TC instead of α-TC, and transgenic line tmt over-accumulating α-TC; exposed to NaCl. The results indicate that salt stress activates β-carboxylation processes in WT plants and in plants with altered TCs accumulation. In α-TC-deficient plants, NaCl causes ACC decrease, but does not change SA, whose concentration remains higher than in α-TC accumulating plants. α/γ-TCs contents influence carbohydrates, poliamines, phenolic (caffeic; ferrulic; cinnamic) acids accumulation patterns. Salinity results in increased detection of the LM5 galactan and LM19 homogalacturonan epitopes in α-TC accumulating plants, and the LM6 arabinan and MAC207 AGP epitopes in α-TC deficient mutants. Parallel, plants with altered TCs composition show decreased both the cell turgor and elastic modulus determined at the individual cell level. α-TC deficient plants reveal lower values of cell turgor and elastic modulus, but higher cell hydraulic conductivity than α-TC accumulating plants. Under salt stress, α-TC shows stronger regulatory effect than γ-TC through the impact on chloroplastic biosynthetic pathways and ROS/osmotic-modulating compounds. © 2020 Elsevier Masson SAS},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85062693923,

title = {Topological traits of a cellular pattern versus growth rate anisotropy in radish roots},

author = { A. Piekarska-Stachowiak and J. Szymanowska-Pułka and I.W. Potocka and M. Lipowczan},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062693923&doi=10.1007%2fs00709-019-01362-6&partnerID=40&md5=cccf6583996ca648bc09a4c2447b52e2},

doi = {10.1007/s00709-019-01362-6},

issn = {0033183X},

year  = {2019},

date = {2019-01-01},

journal = {Protoplasma},

volume = {256},

number = {4},

pages = {1037-1049},

publisher = {Springer-Verlag Wien},

abstract = {The topology of a cellular pattern, which means the spatial arrangement of cells, directly corresponds with cell packing, which is crucial for tissue and organ functioning. The topological features of cells that are typically analyzed are the number of their neighbors and the cell area. To date, the objects of most topological studies have been the growing cells of the surface tissues of plant and animal organs. Some of these researches also provide verification of Lewis’s Law concerning the linear correlation between the number of neighboring cells and the cell area. Our aim was to analyze the cellular topology and applicability of Lewis’s Law to an anisotropically growing plant organ. The object of our study was the root apex of radish. Based on the tensor description of plant organ growth, we specified the level of anisotropy in specific zones (the root proper; the columella of the cap and the lateral parts of the cap) and in specific types of both external (epidermis) and internal tissues (stele and ground tissue) of the apex. The strongest anisotropy occurred in the root proper, while both zones of the cap showed an intermediate level of anisotropy of growth. Some differences in the topology of the cellular pattern in the zones were also detected; in the root proper, six-sided cells predominated, while in the root cap columella and in the lateral parts of the cap, most cells had five neighbors. The correlation coefficient rL between the number of neighboring cells and the cell area was high in the apex as a whole as well as in all of the zones except the root proper and in all of the tissue types except the ground tissue. In general, Lewis’s Law was fulfilled in the anisotropically growing radish root apex. However, the level of the applicability (rL value) of Lewis’s Law was negatively correlated with the level of the anisotropy of growth, which may suggest that in plant organs in the regions of anisotropic growth, the number of neighboring cells is less dependent on the cell size. © 2019, The Author(s).},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85056283476,

title = {Morphological responses of plant roots to mechanical stress},

author = { I.W. Potocka and J. Szymanowska-Pułka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056283476&doi=10.1093%2faob%2fmcy010&partnerID=40&md5=e8ccd6024d25ddf1845d77649f9171a8},

doi = {10.1093/aob/mcy010},

issn = {10958290},

year  = {2018},

date = {2018-01-01},

journal = {Annals of botany},

volume = {122},

number = {5},

pages = {711-723},

publisher = {NLM (Medline)},

abstract = {Background: Roots are continuously exposed to mechanical pressure and this often results in their morphological modification. Most obvious are changes in the overall form of the root system as well as in the shapes of particular roots. These changes are often accompanied by modifications of the cell pattern and cell morphology. Scope: This review focuses on the morphological responses of roots to mechanical stress. Results of early and recent experiments in which roots have been exposed to mechanical pressure are assembled, analysed and discussed. Research applying different experimental sets, obstacles, media of various compactness and structure are reviewed. An effect of the combination of mechanical stresses with other abiotic stresses on roots, and results of estimating the force exerted by the roots are briefly discussed. Possible consequences of the cell pattern rearrangements are considered. Conclusions: Several modifications in root morphology are commonly reported: (1) decreased root size, (2) radial swelling accompanied by increased radial dimension of the cortex cell layers and (3) enhanced cap cell sloughing. Nevertheless, because of differences between species and individual plants, a universal scenario for root morphological changes resulting from externally applied pressures is not possible. Thus, knowledge of the root response to mechanical impedance remains incomplete. Studies on the mechanical properties of the root as well as on possible modifications in cell wall structure and composition as the elements responsible for the mechanical properties of the plant tissue are required to understand the response of root tissue as a biomaterial.},

note = {48},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85046167557,

title = {Spatio-temporal localization of selected pectic and arabinogalactan protein epitopes and the ultrastructural characteristics of explant cells that accompany the changes in the cell fate during somatic embryogenesis in Arabidopsis thaliana},

author = { I.W. Potocka and K. Godel and I. Dobrowolska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046167557&doi=10.1016%2fj.plaphy.2018.04.032&partnerID=40&md5=df32e859e10de06c7f7f8dfa7fbcbe6d},

doi = {10.1016/j.plaphy.2018.04.032},

issn = {09819428},

year  = {2018},

date = {2018-01-01},

journal = {Plant Physiology and Biochemistry},

volume = {127},

pages = {573-589},

publisher = {Elsevier Masson SAS},

abstract = {During somatic embryogenesis (SE), explant cells undergo changes in the direction of their differentiation, which lead to diverse cell phenotypes. Although the genetic bases of the SE have been extensively studied in Arabidopsis thaliana, little is known about the chemical characteristics of the wall of the explant cells, which undergo changes in the direction of differentiation. Thus, we examined the occurrence of selected pectic and AGP epitopes in explant cells that display different phenotypes during SE. Explants examinations have been supplemented with an analysis of the ultrastructure. The deposition of selected pectic and AGP epitopes in somatic embryos was determined. Compared to an explant at the initial stage, a/embryogenic/totipotent and meristematic/pluripotent cells were characterized by a decrease in the presence of AGP epitopes, b/the presence of AGP epitopes in differentiated cells was similar, and c/an increase of analyzed epitopes was detected in the callus cells. Totipotent cells could be distinguished from pluripotent cells by: 1/the presence of the LM2 epitope in the latest one, 2/the appearance of the JIM16 epitope in totipotent cells, and 3/the more abundant presence of the JIM7 epitope in the totipotent cells. The LM5 epitope characterized the wall of the cells that were localized within the mass of embryogenic domain. The JIM8, JIM13 and JIM16 AGP epitopes appeared to be the most specific for the callus cells. The results indicate a relationship between the developmental state of the explant cells and the chemical composition of the cell walls. © 2018 The Authors},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84898724460,

title = {Various scenarios of the cell pattern formation in Arabidopsis lateral root},

author = { J. Biaek and I.W. Potocka and J. Szymanowska-Pułka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84898724460&doi=10.5586%2fasbp.2014.005&partnerID=40&md5=abbd5af783455561364281d3cc5f2a49},

doi = {10.5586/asbp.2014.005},

issn = {00016977},

year  = {2014},

date = {2014-01-01},

journal = {Acta Societatis Botanicorum Poloniae},

volume = {83},

number = {1},

pages = {85-89},

publisher = {Polish Botanical Society},

abstract = {During lateral root (LR) development a coordinate sequence of cell divisions, accompanied by a change of the organ form takes place. Both the order of anatomical events and morphological features may vary for individual primordia. At early stages of LR primordia development oblique division walls are inserted in cells that are symmetrically located on both sides of the axis of the developing LR primordium, and thereby allow for the protrusion of the LR. We hypothesize that both oblique cell wall insertion and continuous changes in primordium form could be a consequence of a local change in stress distribution in the region of the LR initiation. © 2014 The Author(s).},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84879842940,

title = {Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana},

author = { K. Sala and I.W. Potocka and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84879842940&doi=10.1007%2fs10535-013-0304-6&partnerID=40&md5=6d23dd96351eb5038e6f5123ed9959f0},

doi = {10.1007/s10535-013-0304-6},

issn = {00063134},

year  = {2013},

date = {2013-01-01},

journal = {Biologia Plantarum},

volume = {57},

number = {3},

pages = {410-416},

abstract = {The aim of the present study was to describe the occurrence of three pectic epitopes, recognized by JIM7, LM19, and LM5 antibodies, during somatic (SE) and zygotic (ZE) embryogenesis in Arabidopsis thaliana. The epitopes recognized by JIM7 and LM19 antibodies showed different distributions during SE stages. Moreover, in the early stages of somatic embryo development, a cytoplasmic occurrence of LM19 epitope was detected. Distribution of a pectic epitope recognized by LM5 antibody corresponded to a vascular system differentiation pattern. Occurrence of LM5 epitope was the same in both zygotic and somatic embryos and often restricted to newly synthesized walls of two adjacent cells. These data suggest that both low and high methyl-esterified pectins (recognized by LM19 and JIM7 antibodies; respectively) are developmentally regulated during SE stages and (1→4)-β-D-galactan epitope (recognized by LM5 antibody) may play a role in cell cytokinesis. © 2013 Springer Science+Business Media Dordrecht.},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84867669808,

title = {Distribution of lipid transfer protein 1 (LTP1) epitopes associated with morphogenic events during somatic embryogenesis of Arabidopsis thaliana},

author = { I.W. Potocka and T.C. Baldwin and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867669808&doi=10.1007%2fs00299-012-1314-0&partnerID=40&md5=fcae8a1ea519f971e385aba750e03a90},

doi = {10.1007/s00299-012-1314-0},

issn = {07217714},

year  = {2012},

date = {2012-01-01},

journal = {Plant Cell Reports},

volume = {31},

number = {11},

pages = {2031-2045},

abstract = {Using immunocytochemical methods, at both the light and electron microscopic level, we have investigated the spatial and temporal distribution of lipid transfer protein 1 (LTP1) epitopes during the induction of somatic embryogenesis in explants of Arabidopsis thaliana. Immunofluorescence labelling demonstrated the presence of high levels of LTP1 epitopes within the proximal regions of the cotyledons (embryogenic regions) associated with particular morphogenetic events, including intense cell division activity, cotyledon swelling, cell loosening and callus formation. Precise analysis of the signal localization in protodermal and subprotodermal cells indicated that cells exhibiting features typical of embryogenic cells were strongly labelled, both in walls and the cytoplasm, while in the majority of meristematic-like cells no signal was observed. Staining with lipophilic dyes revealed a correlation between the distribution of LTP1 epitopes and lipid substances within the cell wall. Differences in label abundance and distribution between embryogenic and non-embryogenic regions of explants were studied in detail with the use of immunogold electron microscopy. The labelling was strongest in both the outer periclinal and anticlinal walls of the adaxial, protodermal cells of the proximal region of the cotyledon. The putative role(s) of lipid transfer proteins in the formation of lipid lamellae and in cell differentiation are discussed. Key message Occurrence of lipid transfer protein 1 epitopes in Arabidopsis explant cells accompanies changes in cell fate and may be correlated with the deposition of lipid substances in the cell walls. © 2012 The Author(s).},

note = {31},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84871907416,

title = {Principal growth directions in development of the lateral root in Arabidopsis thaliana.},

author = { J. Szymanowska-Pułka and I.W. Potocka and J. Karczewski and K. Jiang and J. Nakielski and L.J. Feldman},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84871907416&doi=10.1093%2faob%2fmcs129&partnerID=40&md5=0fe95581b577a117c3987f3991ab8a9e},

doi = {10.1093/aob/mcs129},

issn = {10958290},

year  = {2012},

date = {2012-01-01},

journal = {Annals of botany},

volume = {110},

number = {2},

pages = {491-501},

abstract = {During lateral root development a new meristem is formed within the mother root body. The main objective of this work was to simulate lateral root formation in Arabidopsis thaliana and to study a potential role of the principal directions in this process. Lateral root growth is anisotropic, so that three principal directions of growth can be distinguished within the organ. This suggests a tensorial character of growth and allows for its description by means of the growth tensor method. First features of the cell pattern of developing lateral roots were analysed in A. thaliana and then a tensorial model for growth and division of cells for this case was specified, assuming an unsteady character of the growth field of the organ. Microscopic observations provide evidence that the principal directions of growth are manifested at various developmental stages by oblique cell walls observed in different regions of the primordium. Other significant features observed are atypically shaped large cells at the flanks of young apices, as well as distinct boundaries between the mother root and the primordium. Simulations were performed using a model for growth. In computer-generated sequences the above-mentioned features could be identified. An attempt was made to reconstruct the virtual lateral root that included a consideration of the formation of particular tissue types based on literature data. In the cell pattern of the developing lateral root the principal directions of growth can be recognized through occurrence of oblique cell divisions. In simulation the role of these directions in cell pattern formation was confirmed, only when cells divide with respect to the principal directions can realistic results be obtained.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-80052879600,

title = {Effect of mechanical stress on Zea root apex. I. Mechanical stress leads to the switch from closed to open meristem organization},

author = { I.W. Potocka and J. Szymanowska-Pułka and J. Karczewski and J. Nakielski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052879600&doi=10.1093%2fjxb%2ferr169&partnerID=40&md5=ecf6f060f969f27f7c0019b225fc1597},

doi = {10.1093/jxb/err169},

issn = {00220957},

year  = {2011},

date = {2011-01-01},

journal = {Journal of Experimental Botany},

volume = {62},

number = {13},

pages = {4583-4593},

abstract = {The effect of mechanical stress on the root apical meristem (RAM) organization of Zea mays was investigated. In the experiment performed, root apices were grown through a narrowing of either circular (variant I) or elliptical (variant II) shape. This caused a mechanical impedance distributed circumferentially or from the opposite sides in variant I and II, respectively. The maximal force exerted by the growing root in response to the impedance reached the value of 0.15 N for variant I and 0.08 N for variant II. Significant morphological and anatomical changes were observed. The changes in morphology depended on the variant and concerned diminishing and/or deformation of the cross-section of the root apex, and buckling and swelling of the root. Anatomical changes, similar in both variants, concerned transformation of the meristem from closed to open, an increase in the number of the cell layers at the pole of the root proper, and atypical oblique divisions of the root cap cells. After leaving the narrowing, a return to both typical cellular organization and morphology of the apex was observed. The results are discussed in terms of three aspects: the morphological response, the RAM reorganization, and mechanical factors. Assuming that the orientation of division walls is affected by directional cues of a tensor nature, the changes mentioned may indicate that a pattern of such cues is modified when the root apex passes through the narrowing, but its primary mode is finally restored. © 2011 The Author(s).},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}