@article{2-s2.0-85191660822,

title = {The potential of enhanced phytoremediation to clean up multi-contaminated soil – insights from metatranscriptomics},

author = { M. Pacwa-Płociniczak and A. Kumor and M. Bukowczan and A. Sinkkonen and M.I. Roslund and T. Płociniczak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85191660822&doi=10.1016%2fj.micres.2024.127738&partnerID=40&md5=8cf241c76fa93518298378a353af22cc},

doi = {10.1016/j.micres.2024.127738},

year  = {2024},

date = {2024-01-01},

journal = {Microbiological Research},

volume = {284},

publisher = {Elsevier GmbH},

abstract = {This study aimed to (i) investigate the potential for enhanced phytoremediation to remove contaminants from soil historically co-contaminated with petroleum hydrocarbons (PHs) and heavy metals (HMs) and (ii) analyze the expression of crucial bacterial genes and whole metatranscriptomics profiles for better understanding of soil processes during applied treatment. Phytoremediation was performed using Zea mays and supported by the Pseudomonas qingdaonensis ZCR6 strain and a natural biofertilizer: meat and bone meal (MBM). In previous investigations, mechanisms supporting plant growth and PH degradation were described in the ZCR6 strain. Here, ZCR6 survived in the soil throughout the experiment, but the efficacy of PH removal from all soils fertilized with MBM reached 32 % regardless of the bacterial inoculation. All experimental groups contained 2 % (w/w) MBM. The toxic effect of this amendment on plants was detected 30 days after germination, irrespective of ZCR6 inoculation. Among the 17 genes tested using the qPCR method, only expression of the acdS gene, encoding 1-aminocyclopropane-1-carboxylic acid deaminase, and the CYP153 gene, encoding cytochrome P450-type alkane hydroxylase, was detected in soils. Metatranscriptomic analysis of soils indicated increased expression of methane particulated ammonia monooxygenase subunit A (pmoA-amoA) by Nitrosomonadales bacteria in all soils enriched with MBM compared to the non-fertilized control. We suggest that the addition of 2 % (w/w) MBM caused the toxic effect on plants via the rapid release of ammonia, and this led to high pmoA-amoA expression. In parallel, due to its wide substrate specificity, enhanced bacterial hydrocarbon removal in MBM-treated soils was observed. The metatranscriptomic results indicate that MBM application should be considered to improve bioremediation of soils polluted with PHs rather than phytoremediation. However, lower concentrations of MBM could be considered for phytoremediation enhancement. From a broader perspective, these results indicated the superior capability of metatranscriptomics to investigate the microbial mechanisms driving various bioremediation techniques. © 2024 Elsevier GmbH},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85192578545,

title = {Comparative Analysis of Fatty Acids Concentration in Liver and Muscle Tissues of Rats and Mice},

author = { M. Kłosok and D. Gendosz de Carrillo and P. Łaszczyca and T. Płociniczak and H. Jedrzejowska-Szypulka and T. Sawczyn},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85192578545&doi=10.3390%2fapp14083192&partnerID=40&md5=84a883599983a8ebcaf70bda39238b4b},

doi = {10.3390/app14083192},

year  = {2024},

date = {2024-01-01},

journal = {Applied Sciences (Switzerland)},

volume = {14},

number = {8},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {This study conducted a comparative analysis of fatty acids (FAs) concentration derived from polar (PL) and non-polar (NPL) lipids in the liver and muscle tissues of rats and mice. The objective was to elucidate species-specific differences in tissue FA distribution. Employing targeted GC/MS-based methodology, the study aimed to provide insights into conserved and species-specific aspects of lipid metabolism, thereby enhancing future experimental design, linked with comprehension of the interactions between diet, metabolism, and health. Results revealed markedly higher levels of total fatty acids (TFAs) derived from PL in mice skeletal muscle compared to rats and elevated saturated fatty acids (SFAs) levels in mice. Unsaturated fatty acid levels, mainly monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), were substantially higher in mice muscle across all lipid classes, resulting in a higher PUFA/TFA ratio in mice muscle. Detailed analysis of specific unsaturated fatty acids (C16:1; C18:1n9c; C18:2n6c; C22:6n3) indicated elevated levels in mice relative to rats. Conversely, rats exhibited higher SFA levels derived from the NPL fraction in the liver, particularly in myristic (C14:0), stearic (C18:0), and tricosanoic (C23:0) acids. Mice liver PL fractions displayed significantly elevated unsaturated FA levels, with notably higher MUFAs and lower PUFAs in NPL fractions compared to rats. Analysis of specific unsaturated FAs revealed higher levels of palmitoleic acid (C16:1) in mice, while rats exhibited increased linoleic (C18:2n6c) and linolenic (C18:3n3) acids. In conclusion, significant differences in FAs tissue distribution between rats and mice underscore the importance of considering species-specific FAs variations when utilizing these animal models and interpreting experimental results related to FA metabolism. © 2024 by the authors.},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85171857887,

title = {Multi-omics insights into the positive role of strigolactone perception in barley drought response},

author = { A. Daszkowska-Golec and D. Mehta and R.G. Uhrig and A.J. Brąszewska-Zalewska and O. Novák and I.M. Fontana and M. Melzer and T. Płociniczak and M. Marzec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85171857887&doi=10.1186%2fs12870-023-04450-1&partnerID=40&md5=b4cdbd82982d8b64f033dd023c188683},

doi = {10.1186/s12870-023-04450-1},

issn = {14712229},

year  = {2023},

date = {2023-01-01},

journal = {BMC Plant Biology},

volume = {23},

number = {1},

publisher = {BioMed Central Ltd},

abstract = {Background: Drought is a major environmental stress that affects crop productivity worldwide. Although previous research demonstrated links between strigolactones (SLs) and drought, here we used barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to scrutinize the SL-dependent mechanisms associated with water deficit response. Results: We have employed a combination of transcriptomics, proteomics, phytohormonomics analyses, and physiological data to unravel differences between wild-type and hvd14 plants under drought. Our research revealed that drought sensitivity of hvd14 is related to weaker induction of abscisic acid-responsive genes/proteins, lower jasmonic acid content, higher reactive oxygen species content, and lower wax biosynthetic and deposition mechanisms than wild-type plants. In addition, we identified a set of transcription factors (TFs) that are exclusively drought-induced in the wild-type barley. Conclusions: Critically, we resolved a comprehensive series of interactions between the drought-induced barley transcriptome and proteome responses, allowing us to understand the profound effects of SLs in alleviating water-limiting conditions. Several new avenues have opened for developing barley more resilient to drought through the information provided. Moreover, our study contributes to a better understanding of the complex interplay between genes, proteins, and hormones in response to drought, and underscores the importance of a multidisciplinary approach to studying plant stress response mechanisms. © 2023, BioMed Central Ltd., part of Springer Nature.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85152302401,

title = {Morphology and distribution of biological soil crusts and their potential role in soil-forming processes under dry high-altitude periglacial conditions (Eastern Pamir, Tajikistan)},

author = { M. Mętrak and M. Wilk and I. Jasser and N. Khomutovska and B. Korabiewski and T. Niyatbekov and T. Płociniczak and M. Wrzosek and M. Suska-Malawska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85152302401&doi=10.1016%2fj.geodrs.2023.e00636&partnerID=40&md5=c273d1ce34d69d3fc226e3ea52191e0c},

doi = {10.1016/j.geodrs.2023.e00636},

issn = {23520094},

year  = {2023},

date = {2023-01-01},

journal = {Geoderma Regional},

volume = {33},

publisher = {Elsevier B.V.},

abstract = {Under demanding climatic conditions that limit the development of vascular vegetation, biological soil crusts (BSCs) drive the processes of soil formation and nutrient sequestration. Though BSCs were studied in glacier forelands worldwide, the high-altitude areas with a combination of glaciation/deglaciation and arid or hyperarid climate remain almost unstudied in this respect. Therefore, we provided the first data on BSCs from a glacier foreland in the E Pamir. These characteristics can be crucial for assessing BSC's role in soil-forming processes in dry, high-altitude periglacial environments. During our research, we assessed (1) BSCs’ morphology and distribution; (2) microbial biomass and nutrient retention patterns in morphologically differentiated BSC types, (3) C, N and P accumulation in BSCs biomass in comparison to sub-crust soils; (4) sub-crust soils enrichment in C, N and P in comparison to bare soils and soils under vascular plants; (5) potential origin and transformation degree of organic matter accumulated by BSCs. Our study showed that the distribution and development of BSCs were noticeably restricted, probably due to low temperatures, aridity and intense periglacial processes, resulting in continuous soil surface remodeling. Thus, poorly developed BSCs were the dominating biologically active soil cover type and, thus, most likely the main biological soil-forming factor in the foreland. BSCs accumulated C, N and P in their biomass and enriched their sub-crust soils in these nutrients. The average enrichment observed for soils under advanced crusts was similar to those obtained for soils under vascular plants. In all types of the studied samples, including bare soils, n-alkanes of vascular plant origin dominated, indicating mixing and uniform distribution of organic matter. Over the course of aridification projected for the Pamir Mountains, the BSCs could potentially become the most important player in the accumulation of soil nutrients in this area. However, due to the dominance of the simplest BSC type, soil formation in the Uisu Glacier foreland will be relatively slow. © 2023 The Authors},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85137276108,

title = {A deeper insight into the phytoremediation of soil polluted with petroleum hydrocarbons supported by the Enterobacter ludwigii ZCR5 strain},

author = { M. Pacwa-Płociniczak and A. Byrski and D. Chlebek and M. Prach and T. Płociniczak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85137276108&doi=10.1016%2fj.apsoil.2022.104651&partnerID=40&md5=5d2348787e46acc97c917687638552ee},

doi = {10.1016/j.apsoil.2022.104651},

issn = {09291393},

year  = {2023},

date = {2023-01-01},

journal = {Applied Soil Ecology},

volume = {181},

publisher = {Elsevier B.V.},

abstract = {The Enterobacter ludwigii ZCR5 strain, isolated from the leaves of Zea mays growing in soil cocontaminated with hydrocarbons and heavy metals, was investigated under laboratory conditions for its plant growth-promoting traits, petroleum hydrocarbon (PH) degradation and biosurfactant production abilities and then used in bacterial-assisted phytoremediation. The whole genome of the ZCR5 was sequenced, and the presence of several genes encoding enzymes important for the enhancement of phytoremediation of soil polluted with PHs was detected. Next, the ZCR5 strain was introduced into polluted soil planted with Lolium perenne cv. Pinia. After inoculation, the ZCR5 strain was able to survive in polluted soil and colonized plant tissues. The highest efficacy of PHs removal (30.6 %) was observed for soil bioaugmented with living cells of the ZCR5, whereas in soil treated with bacterial necromass, it reached a value of 17.6 %. During the experimental period, the mechanisms enhancing the phytoremediation of PH-polluted soils were also tested in situ through the quantification of the expression of selected genes. The expression of genes encoding proteins involved in the biosurfactant production and promotion of plant growth by the ZCR5 strain was not detected either in the rhizosphere or in the endosphere of ryegrass. Among the genes involved in the degradation of PH, only CYP153 (in the rhizo- and endo-spheres) and nahAC (in the rhizosphere) were expressed. Despite the fact that we observed a higher efficiency of phytoremediation in the ZCR5-bioaugmented soil than in the controls, we were not able to indicate the bacterial mechanisms responsible for the observed effect of phytoremediation enhancement by the ZCR5 strain. The results obtained in our experiments indicate the necessity to study bacterial-plant interactions during assisted phytoremediation at the more detailed level. Thus the metatranscriptomic analysis of bacterial activity will be performed in the next step. © 2022 The Authors},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85121668492,

title = {Analysis of the genome of the heavy metal resistant and hydrocarbon-degrading rhizospheric pseudomonas qingdaonensis zcr6 strain and assessment of its plant-growth-promoting traits},

author = { D. Chlebek and T. Płociniczak and S. Gobetti and A. Kumor and K.T. Hupert-Kocurek and M. Pacwa-Płociniczak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121668492&doi=10.3390%2fijms23010214&partnerID=40&md5=db0b844c58764bf8192f1cb2aad87623},

doi = {10.3390/ijms23010214},

issn = {16616596},

year  = {2022},

date = {2022-01-01},

journal = {International Journal of Molecular Sciences},

volume = {23},

number = {1},

publisher = {MDPI},

abstract = {The Pseudomonas qingdaonensis ZCR6 strain, isolated from the rhizosphere of Zea mays growing in soil co-contaminated with hydrocarbons and heavy metals, was investigated for its plant growth promotion, hydrocarbon degradation, and heavy metal resistance. In vitro bioassays confirmed all of the abovementioned properties. ZCR6 was able to produce indole acetic acid (IAA), siderophores, and ammonia, solubilized Ca3 (PO4 )2, and showed surface active properties and activity of cellulase and very high activity of 1-aminocyclopropane-1-carboxylic acid deaminase (297 nmol α-ketobutyrate mg−1 h−1 ). The strain degraded petroleum hydrocarbons (76.52% of the initial hydrocarbon content was degraded) and was resistant to Cd, Zn, and Cu (minimal inhibitory concentrations reached 5; 15; and 10 mM metal; respectively). The genome of the ZCR6 strain consisted of 5,507,067 bp, and a total of 5055 genes were annotated, of which 4943 were protein-coding sequences. Annotation revealed the presence of genes associated with nitrogen fixation, phosphate solubilization, sulfur metabolism, siderophore biosynthesis and uptake, synthesis of IAA, ethylene modulation, heavy metal resistance, exopolysaccharide biosynthesis, and organic compound degradation. Complete characteristics of the ZCR6 strain showed its potential multiway properties for enhancing the phytoremediation of co-contaminated soils. To our knowledge, this is the first analysis of the biotechnological potential of the species P. qingdaonensis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85089696682,

title = {Cuticular waxes—A shield of barley mutant in CBP20 (Cap-Binding Protein 20) gene when struggling with drought stress},

author = { A. Daszkowska-Golec and J. Karcz and T. Płociniczak and K. Sitko and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089696682&doi=10.1016%2fj.plantsci.2020.110593&partnerID=40&md5=ad2913c77b8570c0d656e8236defcf56},

doi = {10.1016/j.plantsci.2020.110593},

issn = {01689452},

year  = {2020},

date = {2020-01-01},

journal = {Plant Science},

volume = {300},

publisher = {Elsevier Ireland Ltd},

abstract = {CBP20 (Cap-Binding Protein 20) encodes a small subunit of nuclear Cap-Binding Complex (nCBC) that together with CBP80 binds mRNA cap. We previously described barley hvcbp20.ab mutant that demonstrated higher leaf water content and faster stomatal closure than the WT after drought stress. Hence, we presumed that the better water-saving mechanism in hvcbp20.ab may result from the lower permeability of epidermis that together with stomata action limit the water evaporation under drought stress. We asked whether hvcbp20.ab exhibited any differences in wax load on the leaf surface when subjected to drought in comparison to WT cv. ‘Sebastian’. To address this question, we investigated epicuticular wax structure and chemical composition under drought stress in hvcbp20.ab mutant and its WT. We showed that hvcbp20.ab mutant exhibited the increased deposition of cuticular wax. Moreover, our gene expression results suggested a role of HvCBP20 as a negative regulator of both, the biosynthesis of waxes at the level of alkane-forming, and waxes transportation. Interestingly, we also observed increased wax deposition in Arabidopsis cbp20 mutant exposed to drought, which allowed us to describe the CBP20-regulated epicuticular wax accumulation under drought stress in a wider evolutionarily context. © 2020 Elsevier B.V.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85090893280,

title = {Comparison of two inoculation methods of endophytic bacteria to enhance phytodegradation efficacy of an aged petroleum hydrocarbons polluted soil},

author = { M. Pawlik and T. Płociniczak and S. Thijs and I. Pintelon and J. Vangronsveld and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090893280&doi=10.3390%2fagronomy10081196&partnerID=40&md5=f2f787ff1be30dda008aa9d732e2907f},

doi = {10.3390/agronomy10081196},

issn = {20734395},

year  = {2020},

date = {2020-01-01},

journal = {Agronomy},

volume = {10},

number = {8},

publisher = {MDPI AG},

abstract = {Endophyte-enhanced phytodegradation is a promising technology to clean up polluted soils. To improve the success rate of this nature-based remediation approach, it is important to advance the inoculation method as this has been shown to strongly affect the final outcome. However, studies evaluating inoculation strategies and their effect on hydrocarbon degradation are limited. This study aims to investigate two different manners of endophyte inoculation in Lolium perenne growing in an aged petroleum hydrocarbon polluted soil: (1) direct soil inoculation (SI), and (2) pre-inoculation of the caryopses followed by soil inoculation (PI). Different endophytic bacterial strains, Rhodococcus erythropolis 5WK and Rhizobium sp. 10WK, were applied individually as well as in combination. Depending on the method of inoculation, the petroleum hydrocarbon (PHC) degradation potential was significantly different. The highest PHC removal was achieved after pre-inoculation of ryegrass caryopses with a consortium of both bacterial strains. Moreover, both strains established in the aged-polluted soil and could also colonize the roots and shoots of L. perenne. Importantly, used endophytes showed the selective colonization of the environment compartments. Our findings show that the method of inoculation determines the efficiency of the phytodegradation process, especially the rate of PHC degradation. This study provides valuable information for choosing the most cost-effective and beneficial means to optimize phytodegradation. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85088455072,

title = {Comparative study on multiway enhanced bio- And phytoremediation of aged petroleum-contaminated soil},

author = { N. Ptaszek and M. Pacwa-Płociniczak and M. Noszczyńska and T. Płociniczak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088455072&doi=10.3390%2fagronomy10070947&partnerID=40&md5=3233ddf61e84a0d289d4a54298bf1603},

doi = {10.3390/agronomy10070947},

issn = {20734395},

year  = {2020},

date = {2020-01-01},

journal = {Agronomy},

volume = {10},

number = {7},

publisher = {MDPI AG},

abstract = {Bioremediation and phytoremediation of soil polluted with petroleum hydrocarbons (PHs) are an effective and eco-friendly alternative to physicochemical methods of soil decontamination. These techniques can be supported by the addition of effective strains and/or surface-active compounds. However, to obtain maximum efficacy of bioremediation, the interactions that occur between the microorganisms, enhancement factors and plants need to be studied. Our study aimed to investigate the removal of petroleum hydrocarbons from an aged and highly polluted soil (hydrocarbon content about 2.5%) using multiway enhanced bio- and phytoremediation. For this purpose, 10 enhanced experimental groups were compared to two untreated controls. Among the enhanced experimental groups, the bio- and phytoremediation processes were supported by the endophytic strain Rhodococcus erythropolis CDEL254. This bacterial strain has several plant growth-promoting traits and can degrade petroleum hydrocarbons and produce biosurfactants. Additionally, a rhamnolipid solution produced by Pseudomonas aeruginosa was used to support the total petroleum hydrocarbon loss from soil. After 112 days of incubation, the highest PH removal (31.1%) was observed in soil planted with ryegrass (Lolium perenne L. cv. Pearlgreen) treated with living cells of the CDEL254 strain and rhamnolipid solution. For non-planted experimental groups, the highest PH loss (26.1%) was detected for soil treated with heat-inactivated CDEL254 cells and a rhamnolipid solution. In general, the differences in the efficacy of the 10 experimental groups supported by plants, live/dead cells of the strain tested and rhamnolipid were not statistically significant. However, each of these groups was significantly more effective than the appropriate control groups. The PH loss in untreated (natural attenuation) and soils that underwent phytoremediation reached a value of 14.2% and 17.4%, respectively. Even though the CDEL254 strain colonized plant tissues and showed high survival in soil, its introduction did not significantly increase PH loss compared to systems treated with dead biomass. These results indicate that the development of effective biological techniques requires a customized approach to the polluted site and effective optimization of the methods used. © 2020 by the authors.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85081025496,

title = {Response of rhizospheric and endophytic bacterial communities of white mustard (Sinapis alba) to bioaugmentation of soil with the Pseudomonas sp. H15 strain},

author = { T. Płociniczak and M. Pacwa-Płociniczak and M. Kwaśniewski and K. Chwiałkowska and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081025496&doi=10.1016%2fj.ecoenv.2020.110434&partnerID=40&md5=d8a193f0065138b2d4d3d54523d47078},

doi = {10.1016/j.ecoenv.2020.110434},

issn = {01476513},

year  = {2020},

date = {2020-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {194},

publisher = {Academic Press},

abstract = {A factor that may significantly increase the efficacy of phytoextraction is soil bioaugmentation with specific bacteria, which can alter the composition of rhizospheric and endophytic bacterial communities. The aim of this study was to compare the effect of soil treatment with living (bioaugmentation) and dead (control) cells of the plant growth-promoting metal-resistant endophytic strain Pseudomonas sp. H15 on the bacterial community composition in the rhizo- and endo-sphere of white mustard during enhanced phytoextraction. The bacterial communities in the rhizosphere were dominated (51.7–68.2%) by Proteobacteria, regardless of the soil treatment or sampling point. A temporary increase in the number of sequences belonging to Gammaproteobacteria (up to 37.3%) was only observed 24 h after the soil treatment with living Pseudomonas sp. H15 cells, whereas for the remaining samples, the relative abundance of this class did not exceed 7.1%. The relative abundance of Proteobacteria in the endosphere of the roots, stems, and leaves of white mustard was higher in the control than in bioaugmented plants. The most pronounced dominance of the Gammaproteobacteria sequences was observed in the stems and leaves of the control plants at the first sampling point, which strongly indicates the ability of the plants to rapidly uptake DNA from soil and translocate it to the aboveground parts of the plants. Additionally, the bioaugmentation of the soil caused a diverse shift in the bacterial communities in the rhizo- and endo-sphere of white mustard compared to control. The most distinct differences, which were dependent on the treatment, were observed in the endosphere of plants at the beginning of the experiment and decreased over time. These results indicate that the rhizo- and endo-biome of white mustard reacts to soil bioaugmentation and may influence the efficiency of bacterial-assisted phytoextraction. © 2020 Elsevier Inc.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85058032943,

title = {Metal-tolerant endophytic bacteria associated with Silene vulgaris support the Cd and Zn phytoextraction in non-host plants},

author = { T. Płociniczak and M. Chodór and M. Pacwa-Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058032943&doi=10.1016%2fj.chemosphere.2018.12.018&partnerID=40&md5=22d7bcbc236a88cd13ff51a612ec9e27},

doi = {10.1016/j.chemosphere.2018.12.018},

issn = {00456535},

year  = {2019},

date = {2019-01-01},

journal = {Chemosphere},

volume = {219},

pages = {250-260},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to isolate and characterise metal-resistant endophytic bacteria from the tissues of Silene vulgaris collected within the vicinity of non-ferrous steelworks in Katowice, Upper Silesia, Southern Poland. Twenty-four strains of metal-resistant endophytic bacteria that belong to 15 genera were isolated from the stems and leaves of Silene vulgaris. Most of these strains showed multiple plant growth-promoting capabilities. The most promising strains, Proteus vulgaris H7, Pseudomonas sp. H15, and Pseudomonas helmanticensis H16, were used in a pot experiment, and their impact on the biomass of white mustard and Zn and Cd accumulation was examined. Soil inoculation with the tested strains resulted in a higher fresh biomass of shoots, which increased by 74.5% (Proteus vulgaris H7), 121.7% (Pseudomonas sp. H15), and 142.2% (P. helmanticensis H16) compared to the control plants. The highest phytoextraction enhancement was caused by P. helmanticensis H16, which increased Zn and Cd accumulation in the shoot tissues by 43.8% and 112.6%, respectively. All of the tested strains were detected in the soil at the last sampling points, but only Proteus vulgaris H7 and Pseudomonas sp. H15 were capable of temporary colonisation of the roots of white mustard. None of the inoculants were found in the stems and leaves of the plants during the experimental period. The plant growth-promoting features of the isolates combined with their resistance to heavy metals and high survival in soil after inoculation make these strains good candidates for the promotion of plant growth and increased phytoremediation efficiency. © 2018 Elsevier Ltd},

note = {37},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85057054295,

title = {The effect of bioaugmentation of petroleum-contaminated soil with Rhodococcus erythropolis strains on removal of petroleum from soil},

author = { M. Pacwa-Płociniczak and J. Czapla and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057054295&doi=10.1016%2fj.ecoenv.2018.11.081&partnerID=40&md5=216b4d448e42c30bd416107f744858a2},

doi = {10.1016/j.ecoenv.2018.11.081},

issn = {01476513},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {169},

pages = {615-622},

publisher = {Academic Press},

abstract = {The aim of the study was to assess the impact of inoculation of petroleum-contaminated soil with the hydrocarbon-degrading bacterial strains Rhodococcus erythropolis CD 130 and CD 167 or their consortium on the removal of hydrocarbons from the soil. Additionally, changes in the activity and structure of soil autochthonous bacterial communities were studied. At the end of the experiment, the fastest hydrocarbon removal was seen in the soil treated with the CD 167 strain (38.40%) and was statistically higher compared to the removal of total petroleum hydrocarbons (TPH) observed in soils inoculated with strain CD 130 (29.8%) or bacterial consortium CD 130 + CD 167 (29.72%). The rifampicin-resistant CD 130 and CD 167 strains, introduced as single strains or a consortium, survived in the soil for 42 days. The introduction of gram-positive strains of R. erythropolis primarily caused an increase in the biomass of branched phospholipid fatty acids (PLFAs), characteristic for gram-positive bacteria. Nevertheless, changes in the concentrations of gram-positive and gram-negative PLFA markers were periodic, and at the end of the experiment, significant changes were observed only in the case of the soil bioaugmented with the CD 167 strain. After the bioaugmentation, higher values of substrate-induced respiration (SIR) were observed in all the inoculated soils compared to the non-inoculated control. Nonetheless, after 91 days of incubation, a significant decrease in soil respiration was observed in the soil treated with single CD 130 or CD 167 strains or with their consortium. The number of transcripts of the CYP153 gene obtained on days 91 and 182 reflected the results of the hydrocarbon loss. The level of expression of the alkH gene in experimental soil was estimated and found to be higher than the level of expression of the CYP153 gene but did not coincide with the loss of hydrocarbons. The introduction of strains CD 130, CD 167, or CD 130 + CD 167 caused temporary changes in the composition of the soil autochthonous bacterial community, but it seems that these changes were needed for the enhanced removal of hydrocarbons from this soil. © 2018 Elsevier Inc.},

note = {33},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040236045,

title = {Effect of Silene vulgaris and Heavy Metal Pollution on Soil Microbial Diversity in Long-Term Contaminated Soil},

author = { M. Pacwa-Płociniczak and T. Płociniczak and D. Yu and J.M. Kurola and A. Sinkkonen and Z. Piotrowska-Seget and M. Romantschuk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040236045&doi=10.1007%2fs11270-017-3655-3&partnerID=40&md5=c3554399dc403e2252677ae08796fad4},

doi = {10.1007/s11270-017-3655-3},

issn = {00496979},

year  = {2018},

date = {2018-01-01},

journal = {Water, Air, and Soil Pollution},

volume = {229},

number = {1},

publisher = {Springer International Publishing},

abstract = {In this study, we analysed the impact of heavy metals and plant rhizodeposition on the structure of indigenous microbial communities in rhizosphere and bulk soil that had been exposed to heavy metals for more than 150 years. Samples of the rhizosphere of Silene vulgaris and non-rhizosphere soils 250 and 450 m from the source of emission that had different metal concentrations were collected for analyses. The results showed that soils were collected 250 m from the smelter had a higher number of Cd-resistant CFU compared with the samples that were collected from 450 m, but no significant differences were observed in the number of total and oligotrophic CFU or the equivalent cell numbers between rhizosphere and non-rhizosphere soils that were taken 250 and 450 m from the emitter. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis of the denaturing gradient gel electrophoresis (DGGE) profiles, as well as a cluster analysis that was generated on the phospholipid fatty acid (PLFA) profiles, showed that the bacterial community structure of rhizosphere soils depended more on the plant than on the distance and metal concentrations. The sequencing of the 16S rDNA fragments that were excised from the DGGE gel revealed representatives of the phyla Bacteroidetes, Acidobacteria, Gemmatimonadetes, Actinobacteria and Betaproteobacteria in the analysed soil with a predominance of the first three groups. The obtained results demonstrated that the presence of S. vulgaris did not affect the number of CFUs, except for those of Cd-resistant bacteria. However, the presence of S. vulgaris altered the soil bacterial community structure, regardless of the sampling site, which supported the thesis that plants have a higher impact on soil microbial community than metal contamination. © 2017, The Author(s).},

note = {34},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85034604138,

title = {The abundance of health-associated bacteria is altered in PAH polluted soils - Implications for health in urban areas?},

author = { A. Parajuli and M. Grönroos and S. Kauppi and T. Płociniczak and M.I. Roslund and P. Galitskaya and O.H. Laitinen and H. Hyöty and A. Jumpponen and R. Strömmer and M. Romantschuk and N. Hui and A. Sinkkonen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85034604138&doi=10.1371%2fjournal.pone.0187852&partnerID=40&md5=daf04338cd62c181266837cb929913e3},

doi = {10.1371/journal.pone.0187852},

issn = {19326203},

year  = {2017},

date = {2017-01-01},

journal = {PLoS ONE},

volume = {12},

number = {11},

publisher = {Public Library of Science},

abstract = {Long-term exposure to polyaromatic hydrocarbons (PAHs) has been connected to chronic human health disorders. It is also well-known that i) PAH contamination alters soil bacterial communities, ii) human microbiome is associated with environmental microbiome, and iii) alteration in the abundance of members in several bacterial phyla is associated with adverse or beneficial human health effects. We hypothesized that soil pollution by PAHs altered soil bacterial communities that had known associations with human health. The rationale behind our study was to increase understanding and potentially facilitate reconsidering factors that lead to health disorders in areas characterized by PAH contamination. Large containers filled with either spruce forest soil, pine forest soil, peat, or glacial sand were left to incubate or contaminated with creosote. Biological degradation of PAHs was monitored using GC-MS, and the bacterial community composition was analyzed using 454 pyrosequencing. Proteobacteria had higher and Actinobacteria and Bacteroidetes had lower relative abundance in creosote contaminated soils than in non-contaminated soils. Earlier studies have demonstrated that an increase in the abundance of Proteobacteria and decreased abundance of the phyla Actinobacteria and Bacteroidetes are particularly associated with adverse health outcomes and immunological disorders. Therefore, we propose that pollution-induced shifts in natural soil bacterial community, like in PAH-polluted areas, can contribute to the prevalence of chronic diseases. We encourage studies that simultaneously address the classic “adverse toxin effect” paradigm and our novel “altered environmental microbiome” hypothesis. © 2017 Parajuli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {30},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85019267226,

title = {Improvement of phytoremediation of an aged petroleum hydrocarbon-contaminated soil by Rhodococcus erythropolis CD 106 strain},

author = { T. Płociniczak and E. Fic and M. Pacwa-Płociniczak and M. Pawlik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019267226&doi=10.1080%2f15226514.2016.1278420&partnerID=40&md5=2ccee0f9bdf71f304543948e2d5fd67f},

doi = {10.1080/15226514.2016.1278420},

issn = {15226514},

year  = {2017},

date = {2017-01-01},

journal = {International Journal of Phytoremediation},

volume = {19},

number = {7},

pages = {614-620},

publisher = {Taylor and Francis Inc.},

abstract = {The aim of this study was to assess the impact of soil inoculation with the Rhodococcus erythropolis CD 106 strain on the effectiveness of the phytoremediation of an aged hydrocarbon-contaminated [approx. 1% total petroleum hydrocarbon (TPH)] soil using ryegrass (Lolium perenne). The introduction of CD 106 into the soil significantly increased the biomass of ryegrass and the removal of hydrocarbons in planted soil. The fresh weight of the shoots and roots of plants inoculated with CD 106 increased by 49% and 30%, respectively. After 210 days of the experiment, the concentration of TPH was reduced by 31.2%, whereas in the planted, non-inoculated soil, it was reduced by 16.8%. By contrast, the concentration of petroleum hydrocarbon decreased by 18.7% in non-planted soil bioaugmented with the CD 106 strain. The rifampicin-resistant CD 106 strain survived after inoculation into soil and was detected in the soil during the entire experimental period, but the number of CD 106 cells decreased constantly during the enhanced phytoremediation and bioaugmentation experiments. The plant growth-promoting and hydrocarbon-degrading properties of CD 106, which are connected with its long-term survival and limited impact on autochthonous microflora, make this strain a good candidate for improving the phytoremediation efficiency of soil contaminated with hydrocarbons. © 2017 Taylor & Francis Group, LLC.},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021152716,

title = {Mutation in HvCBP20 (Cap binding protein 20) adapts barley to drought stress at phenotypic and transcriptomic levels},

author = { A. Daszkowska-Golec and A. Collin and M. Marzec and M. Słota and M.M. Kurowska and M. Gajecka and P. Gajewska and T. Płociniczak and K. Sitko and A. Pacak and Z. Szweykowska-Kulinska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021152716&doi=10.3389%2ffpls.2017.00942&partnerID=40&md5=769fb24d1f52b6fc895f399773d7ea69},

doi = {10.3389/fpls.2017.00942},

issn = {1664462X},

year  = {2017},

date = {2017-01-01},

journal = {Frontiers in Plant Science},

volume = {8},

publisher = {Frontiers Media S.A.},

abstract = {CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the HvCBP20 gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the hvcbp20.ab mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the hvcbp20.ab mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of hvcbp20.ab mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response. © 2017 Daszkowska-Golec, Skubacz, Marzec, Slota, Kurowska, Gajecka, Gajewska, Płociniczak, Sitko, Pacak, Szweykowska-Kulinska and Szarejko.},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84978481147,

title = {A modified weighted mixture model for the interpretation of spatial and temporal changes in the microbial communities in drinking water reservoirs using compositional phospholipid fatty acid data},

author = { I. Stanimirova and A. Woźnica and T. Płociniczak and M. Kwaśniewski and J. Karczewski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978481147&doi=10.1016%2fj.talanta.2016.07.006&partnerID=40&md5=e0f69a1e3ca875379b031e8d2d369b65},

doi = {10.1016/j.talanta.2016.07.006},

issn = {00399140},

year  = {2016},

date = {2016-01-01},

journal = {Talanta},

volume = {160},

pages = {148-156},

publisher = {Elsevier B.V.},

abstract = {The aim of this work was to check whether a methodology based on the analysis of data that contain the entire phospholipid fatty acid, PLFA, compositions of water samples can be successfully used to interpret spatial and temporal changes in the microbial communities in water reservoirs. The proposed methodology consists of the construction of a modified weighted multivariate mixture model for the PLFA profiles of the water samples collected in a given monitoring campaign and the identification of latent PLFA components through a comparison with the known PLFA profiles of some cultivated or non-cultivated microbial communities. A 16S rDNA analysis of some of the selected water samples in the monitoring campaign was performed in order to verify the results of the PLFA analysis. The results showed that the proposed methodology can be useful for a dynamic and sensitive evaluation of changes in the microbial quality of water before and after flash flooding and can help in taking a decision regarding further risk assessment. © 2016 Elsevier B.V.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84975509465,

title = {Endophytic and rhizosphere bacteria associated with the roots of the halophyte Salicornia europaea L. - community structure and metabolic potential},

author = { S. Szymańska and T. Płociniczak and Z. Piotrowska-Seget and K. Hrynkiewicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975509465&doi=10.1016%2fj.micres.2016.05.012&partnerID=40&md5=c211d4eda85ae3e4d7080ee027477f32},

doi = {10.1016/j.micres.2016.05.012},

issn = {09445013},

year  = {2016},

date = {2016-01-01},

journal = {Microbiological Research},

volume = {192},

pages = {37-51},

publisher = {Elsevier GmbH},

abstract = {The main objective of our study was to assess density and diversity of rhizosphere (R) and endophytic (E) microorganisms associated with the halophyte S. europaea. Microorganisms were isolated from two saline sites (S1: 55 dS m-1; anthropogenic origin; S2: 112 dS m-1; natural salinity) located in central Poland and compared with microbial populations in the soil (S) using culture-independent (phospholipid fatty acids analysis; PLFA) and culture-dependent techniques. The endophytic and rhizosphere bacteria were identified and screened for nifH and acdS genes, and their metabolic properties were assessed. Strains with the potential to promote plant growth were selected for further study. PLFA analysis revealed that Gram-negative bacteria were dominant at both saline test sites; the total microbial biomass depended on the site (S1 < S2) and the zone of isolation (R > E > S). In contrast, culture-dependent techniques revealed that Gram-positive bacteria (Actinobacteria and Firmicutes) were dominant (S1: E-77.3%; R-86.3% and S2: E-59.1%; R-87.5%). Proteobacteria were observed in the rhizosphere at the lowest frequency (S1: 13.7% and S2: 12.5%). Greater salinity decreased the range and specificity of metabolic activity among the endophytes. These conditions also resulted in a broader spectrum of metabolic abilities in rhizobacteria; however, these metabolic processes were present at lower levels. © 2016 Elsevier GmbH.},

note = {42},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84949845363,

title = {Isolation of hydrocarbon-degrading and biosurfactant-producing bacteria and assessment their plant growth-promoting traits},

author = { M. Pacwa-Płociniczak and T. Płociniczak and J. Iwan and M. Zarska and M. Chorazewski and M. Dzida and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949845363&doi=10.1016%2fj.jenvman.2015.11.058&partnerID=40&md5=a87f69d8c6d64170f34a59d83a5c2740},

doi = {10.1016/j.jenvman.2015.11.058},

issn = {03014797},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Environmental Management},

volume = {168},

pages = {175-184},

publisher = {Academic Press},

abstract = {Forty-two hydrocarbon-degrading bacterial strains were isolated from the soil heavily contaminated with petroleum hydrocarbons. Forty-one strains were identified based on their whole-cell fatty acid profiles using the MIDI-MIS method. Thirty-three of them belong to species Rhodococcus erythropolis, while the others to the genera Rahnella (4), Serratia (3) and Proteus (1). Isolates were screened for their ability to produce biosurfactants/bioemulsifiers. For all of them the activity of several mechanisms characteristic for plant growth-promoting bacteria was also determined. In order to investigate surface active and emulsifying abilities of isolates following methods: oil-spreading, blood agar, methylene blue agar and determination of emulsification index, were used. Among studied bacteria 12 strains (CD 112; CD 126; CD 131; CD 132; CD 135; CD 147; CD 154; CD 155; CD 158; CD 161; CD 166 and CD 167) have been chosen as promising candidates for the production of biosurfactants and/or bioemulsifiers. Among them 2 strains (R. erythropolis CD 126 and Rahnella aquatilis CD 132) had the highest potential to be used in the bioaugmentation of PH-contaminated soil. Moreover, 15 of tested strains (CD 105; CD 106; CD 108; CD 111; CD 116; CD 120; CD 124; CD 125; CD 130; CD 132; CD 134; CD 154; CD 156; CD 161 and CD 170) showed the activity of four mechanisms (ACC deaminase activity; IAA and siderophore production; phosphate solubilization) considered to be characteristic for plant growth-promoting bacteria. Two of them (R. erythropolis CD 106 and R. erythropolis CD 111) showed the highest activity of above-mentioned mechanisms and thus are considered as promising agents in microbe assisted phytoremediation. © 2015 Elsevier Ltd.},

note = {44},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84960129136,

title = {Rhizospheric bacterial strain brevibacterium casei MH8a colonizes plant tissues and enhances Cd, Zn, Cu phytoextraction by white mustard},

author = { T. Płociniczak and A. Sinkkonen and M. Romantschuk and S. Sułowicz and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960129136&doi=10.3389%2ffpls.2016.00101&partnerID=40&md5=e5f857a1ee07a62caa63d2653e3266aa},

doi = {10.3389/fpls.2016.00101},

issn = {1664462X},

year  = {2016},

date = {2016-01-01},

journal = {Frontiers in Plant Science},

volume = {7},

number = {FEB2016},

publisher = {Frontiers Media S.A.},

abstract = {Environmental pollution by heavy metals has become a serious problem in the world. Phytoextraction, which is one of the plant-based technologies, has attracted the most attention for the bioremediation of soils polluted with these contaminants. The aim of this study was to determine whether the multiple-tolerant bacterium, Brevibacterium casei MH8a isolated from the heavy metal-contaminated rhizosphere soil of Sinapis alba L., is able to promote plant growth and enhance Cd, Zn, and Cu uptake by white mustard under laboratory conditions. Additionally, the ability of the rifampicin-resistant spontaneous mutant of MH8a to colonize plant tissues and its mechanisms of plant growth promotion were also examined. In order to assess the ecological consequences of bioaugmentation on autochthonous bacteria, the phospholipid fatty acid (PLFA) analysis was used. The MH8a strain exhibited the ability to produce ammonia, 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid and HCN but was not able to solubilize inorganic phosphate and produce siderophores. Introduction of MH8a into soil sigNificantly increaSed S. alba biomass and the accumulation of Cd (208%), Zn (86%), and Cu (39%) in plant shoots in comparison with those grown in non-inoculated soil. Introduced into the soil, MH8a was able to enter the plant and was found in the roots and leaves of inoculated plants thus indicating its endophytic features. PLFA analysis revealed that the MH8a that was introduced into soil had a temporary influence on the structure of the autochthonous bacterial communities. The plant growth-promoting features of the MH8a strain and its ability to enhance the metal uptake by white mustard and its long-term survival in soil as well as its temporary impact on autochthonous microorganisms make the strain a suitable candidate for the promotion of plant growth and the efficiency of phytoextraction. © 2016, Płociniczak, Sinkkonen, Romantschuk, Sułowicz and Piotrowska-Seget.},

note = {38},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84949965050,

title = {Metabolic potential and community structure of endophytic and rhizosphere bacteria associated with the roots of the halophyte Aster tripolium L.},

author = { S. Szymańska and T. Płociniczak and Z. Piotrowska-Seget and M. Złoch and S. Ruppel and K. Hrynkiewicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949965050&doi=10.1016%2fj.micres.2015.09.007&partnerID=40&md5=76e362b94f74e4bc57cf0d1b286542e2},

doi = {10.1016/j.micres.2015.09.007},

issn = {09445013},

year  = {2016},

date = {2016-01-01},

journal = {Microbiological Research},

volume = {182},

pages = {68-79},

publisher = {Elsevier GmbH},

abstract = {The submitted work assumes that the abundance and diversity of endophytic and rhizosphere microorganisms co-existing with the halophytic plant Aster tripolium L. growing in a salty meadow in the vicinity of a soda factory (central Poland) represent unique populations of cultivable bacterial strains.Endophytic and rhizosphere bacteria were (i) isolated and identified based on 16S rDNA sequences; (ii) screened for nifH and acdS genes; and (iii) analyzed based on selected metabolic properties. Moreover, total microbial biomass and community structures of the roots (endophytes), rhizosphere and soil were evaluated using a cultivation-independent technique (PLFA) to characterize plant-microbial interactions under natural salt conditions.The identification of the isolated strains showed domination by Gram-positive bacteria (mostly Bacillus spp.) both in the rhizosphere (90.9%) and roots (72.7%) of A. tripolium. Rhizosphere bacterial strains exhibited broader metabolic capacities, while endophytes exhibited higher specificities for metabolic activity. The PLFA analysis showed that the total bacterial biomass decreased in the following order (rhizosphere < soil < endophytes) and confirmed the dominance of Gram-positive endophytic bacteria in the roots of the halophyte.The described strain collection provides a valuable basis for a subsequent applications of bacteria in improvement of site adaptation of plants in saline soils. © 2015 Elsevier GmbH.},

note = {46},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84942428396,

title = {Endophytic bacteria and their role in environmental microbiology, medicine and industry [Bakterie endofityczne i ich znaczenie w mikrobiologii s̈rodowiskowej, medycynie i przemys̈le]},

author = { M. Pawlik and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942428396&partnerID=40&md5=05709f76f31dd8658cc135b2bf0a4e0a},

issn = {00794252},

year  = {2015},

date = {2015-01-01},

journal = {Postepy Mikrobiologii},

volume = {54},

number = {2},

pages = {115-122},

publisher = {Polish Society of Microbiologists},

abstract = {Endophytic bacteria have been known for more than 120 years. They live inside plant tissues without causing any apparent symptoms of disease or negative effects on the host. Each of the nearly 300 000 plant species that exist on the earth is host to one or more endophytes. Only a few of these plants have ever been completely studied relative to their endophytic biology. Consequently, the opportunity to find new and beneficial endophytic microorganisms among the diversity of plants in different ecosystems is considerable. During the long co-evolutionary process with their hosts, endophytes have developed many significant and novel characteristics. The relationships between plants and endophytic bacteria are very close. The endophytes which reside inside plant tissues can be classified as 'obligate' or 'facultative'. The first group is strictly dependent on the host plant for their growth and survival and transmission to other plants. The second group has a stage in their life cycle in which they are able to exist outside the host plant. Recent studies have shown that endophytes accelerate the adaptation of plants to unbalanced environmental conditions such as saline soil, drought, stress caused by pesticides, heavy metals or hydrocarbons. Therefore, the exploitation of the interaction of plant endophytes for the remediation of contaminated soils is a promising area; however, role of these microorganisms is still unclear.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84942902179,

title = {Fitoremediacja terenów skażonych związkami ropopochodnymi z wykorzystaniem bakterii endofitycznych [Phytoremediation of petroleum hydrocarbons-contaminated soils by using endophytic bacteria]},

author = { M. Kukla and B. Cania and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942902179&doi=10.12916%2fprzemchem.2014.355&partnerID=40&md5=c4c47f68c81138f33496465cb92979de},

doi = {10.12916/przemchem.2014.355},

issn = {00332496},

year  = {2014},

date = {2014-01-01},

journal = {Przemysl Chemiczny},

volume = {93},

number = {3},

pages = {355-359},

publisher = {Wydawnictwo SIGMA-NOT},

abstract = {[No abstract available]},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84919597980,

title = {Diversity of endophytic bacteria in Lolium perenne and their potential to degrade petroleum hydrocarbons and promote plant growth},

author = { M. Kukla and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84919597980&doi=10.1016%2fj.chemosphere.2014.05.055&partnerID=40&md5=4f0ba66db9a3d4853e04b6239853f7e5},

doi = {10.1016/j.chemosphere.2014.05.055},

issn = {00456535},

year  = {2014},

date = {2014-01-01},

journal = {Chemosphere},

volume = {117},

number = {1},

pages = {40-46},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to assess the ability of twenty-nine endophytic bacteria isolated from the tissues of ryegrass (Lolium perenne L.) to promote plant growth and the degradation of hydrocarbon. Most of the isolates belonged to the genus Pseudomonas and showed multiple plant growth-promoting abilities. All of the bacteria that were tested exhibited the ability to produce indole-3-acetic acid and were sensitive to streptomycin. These strains were capable of phosphate solubilization (62%), cellulolytic enzyme production (62%), a capacity for motility (55%) as well as for the production of siderophore (45%), ammonium (41%) and hydrogen cyanide (38%). Only five endophytes had the emulsification ability that results from the production of biosurfactants. The 1-aminocyclopropane-1-carboxylate deaminase (ACCD) gene (acdS) was found in ten strains. These bacteria exhibited ACCD activities in the range from 1.8 to 56.6μmol of α-ketobutyrate mg-1h-1, which suggests that these strains may be able to modulate ethylene levels and enhance plant growth. The potential for hydrocarbon degradation was assessed by PCR amplification on the following genes: alkH, alkB, C23O, P450 and pah. The thirteen strains that were tested had the P450 gene but the alkH and pah genes were found only in the Rhodococcus fascians strain (L11). Four endophytic bacteria belonging to Microbacterium sp. and Rhodococcus sp. (L7; S12; S23; S25) showed positive results for the alkB gene. © 2014 Elsevier Ltd.},

note = {64},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84881492403,

title = {Previous exposure advances the degradation of an anthropogenic s-triazine regardless of soil origin},

author = { A. Sinkkonen and S. Kauppi and V. Pukkila and H. Nan and T. Płociniczak and M. Kontro and R. Strömmer and M. Romantschuk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881492403&doi=10.1007%2fs11368-013-0742-y&partnerID=40&md5=ba682c40b00a106b5fee8ec28c23f2a5},

doi = {10.1007/s11368-013-0742-y},

issn = {14390108},

year  = {2013},

date = {2013-01-01},

journal = {Journal of Soils and Sediments},

volume = {13},

number = {8},

pages = {1430-1438},

abstract = {Purpose: Previous investigations-field samplings and laboratory experiments-support the hypothesis that the degradation of s-triazines is enhanced in previously exposed as compared to pristine soils in terrestrial environments. Despite this, bottlenecks of soil sampling and various soil modification practices in microcosm studies have made it difficult to guarantee that previous contamination history enhances contaminant degradation regardless of soil origin in terrestrial ecosystems. We test the hypothesis that the degradation of simazine (2-chloro-4;6-bis(ethylamino)-s-triazine) is enhanced in previously exposed soils as compared to pristine soils in 10 l buckets at the mesocosm scale. Materials and methods: We collected soil at three separate sites consisting of a previously exposed and a pristine field. At every field, soil was collected at three separate plots and simazine degradation (days 0 and 65) and the response to atzB degrader gene primers (days 0 and 110) were followed. We analyzed the results using analysis of covariance (ANCOVA). Previous exposure and field site were assessed as fixed factors and initial simazine concentration and abiotic soil conditions as covariates. Results and discussion: After the 65-day exposure, remaining simazine concentrations depended on previous exposure but not on collection site. The response to atzB gene primers was positive in all mesocosms where simazine degradation had been rapid. Soil moisture, pH, and organic matter content were insignificant. If soil moisture was not included in the ANCOVA model, previous exposure did not appear as a significant factor. Conclusions: The results support the hypothesis that simazine is degraded more rapidly in previously exposed soils as compared to pristine environments, provided that degradation genes are available. Previously exposed soil might be used to enhance the degradation of simazine in recently contaminated terrestrial soils, supposing that the central requirements for microbial growth are adequate. © 2013 Springer-Verlag Berlin Heidelberg.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84876432490,

title = {The effect of soil bioaugmentation with strains of Pseudomonas on Cd, Zn and Cu uptake by Sinapis alba L.},

author = { T. Płociniczak and M. Kukla and R. Watroba and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876432490&doi=10.1016%2fj.chemosphere.2013.03.008&partnerID=40&md5=74246e8c748c10f265f43a70ea814c1c},

doi = {10.1016/j.chemosphere.2013.03.008},

issn = {00456535},

year  = {2013},

date = {2013-01-01},

journal = {Chemosphere},

volume = {91},

number = {9},

pages = {1332-1337},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to assess the ability of selected metal resistant strains of the Pseudomonas genus to increase Zn, Cd and Cu uptake by the metalophyte Sinapis alba L. under laboratory conditions. Moreover, the mechanisms of the plant growth promotion in the tested strains and their impact on the shoots and roots of white mustard biomass were examined. Soil inoculation with the tested strains resulted in higher concentrations of Zn, Cd and Cu in the shoots and roots of the plants in comparison with those grown in non-inoculated soil. The highest phytoextraction enhancement was caused by Pseudomonas fluorescens MH15 which increased Zn, Cd and Cu accumulation in shoot tissue by 60%, 96% and 31%, respectively, in comparison with control plants. Moreover, all the tested strains also exhibited a significant increase of Cd translocation from roots to shoots of the white mustard. Three Pseudomonas putida (MH3; MH6; MH7) and two P. fluorescens biotype G and C (MH9 and MH15; respectively) strains had the ability to produce siderophore, 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid as well as hydrocyanic acid. Additionally, P. putida strains were also capable of solubilizing inorganic phosphate. The ability of the tested strains to increase the metal uptake in white mustard and their plant growth-promoting properties make them good candidates for supporting heavy metal phytoextraction as well as for plant growth promoting. © 2013 Elsevier Ltd.},

note = {42},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84871021485,

title = {Characterization of Enterobacter intermedius MH8b and its use for the enhancement of heavy metals uptake by Sinapis alba L.},

author = { T. Płociniczak and A. Sinkkonen and M. Romantschuk and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84871021485&doi=10.1016%2fj.apsoil.2012.09.009&partnerID=40&md5=9d1ed1c9a279c2356085e1ec5be702a4},

doi = {10.1016/j.apsoil.2012.09.009},

issn = {09291393},

year  = {2013},

date = {2013-01-01},

journal = {Applied Soil Ecology},

volume = {63},

pages = {1-7},

abstract = {The aim of this study was to test whether the multiple-tolerant bacterium Enterobacter intermedius MH8b isolated from metal-contaminated soil is able to promote plant growth and enhance Zn, Cd and Cu uptake by Sinapis alba L. under laboratory conditions. Additionally, the mechanisms of the plant growth promotion and the ecological consequences of bioaugmentation were examined. MH8b exhibited the ability to produce 1-amino-cyclopropane-1-carboxylic acid deaminase, indole 3-acetic acid and hydrocyanic acid and was also able to solubilize inorganic phosphate. Soil inoculation with MH8b significantly increased plant biomass as well as the accumulation of Zn (32%) and Cd (94%) in plant shoots. DGGE and PLFA analysis revealed that the introduced MH8b had only a short-term influence on the structure of indigenous microbial communities. The plant growth-promoting properties of MH8b and its ability to increase the metal uptake connected with its long-term survival and its short-term impact on autochthonous microflora make the strain a good candidate for the promotion of plant growth and phytoremediation efficiency. © 2012 Elsevier B.V.},

note = {47},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85027930598,

title = {Significance of silver birch and bushgrass for establishment of microbial heterotrophic community in a metal-mine spoil heap},

author = { S. Sułowicz and T. Płociniczak and Z. Piotrowska-Seget and J. Kozdrój},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027930598&doi=10.1007%2fs11270-010-0417-x&partnerID=40&md5=d0ac7fb2fd48c67f242175ddba961bc0},

doi = {10.1007/s11270-010-0417-x},

issn = {00496979},

year  = {2011},

date = {2011-01-01},

journal = {Water, Air, and Soil Pollution},

volume = {214},

number = {1-4},

pages = {205-218},

publisher = {Kluwer Academic Publishers},

abstract = {Differences in the culturable fractions of total and metal-tolerant bacteria inhabiting bulk soil of a metal-mine spoil heap and the rhizosphere of silver birch (Betula pendula) or bushgrass (Calamagrostis epigejos), completed with changes in total microbial community structure in the soil, were assessed by MIDI-FAME (fatty acid methyl ester) profiling of whole-cell fatty acids. In addition, the abundance of metal-tolerant populations among the culturable bacterial communities and their identity and the metal-tolerance patterns were determined. The high proportions of Cu- and Zn-tolerant bacteria that ranged from 60.6% to 94.8% were ascertained in the heap sites. Within 31 bacterial isolates obtained, 24 strains were Gram-positive and Arthrobacter, Bacillus, Rathayibacter, Brochothrix, and Staphylococcus represented those identified. Minimum inhibitory concentration (MIC) data indicated that several strains developed multi-metal tolerance, and the highest tolerance to Cu (10 mM) and Zn (12 mM) was found for Pseudomonas putida TP3 and three isolated strains (BS3; TP12; and SL16), respectively. The analysis of FAME profiles obtained from the culturable bacterial communities showed that Gram-positive bacteria predominated in bulk soil of all heap sites. In contrast, the rhizosphere communities showed a lower proportion of the Gram-positive group, especially for silver birch. For the total microbial community, mostly Gram-negative bacteria (e.g.; Pseudomonas) inhabited the heap sites. The results suggest that the quantitative and qualitative development of heterotrophic microbiota in the soil of the metal-mine spoil heap seems to be site-dependent (i.e.; rhizosphere vs. bulk soil), according to differences in the site characteristics (e.g.; enrichment of nutrients and total metal concentrations) and impact of plant species. © 2010 Springer Science+Business Media B.V.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-78049346290,

title = {Response of bacteria to heavy metals measured as changes in FAME profiles},

author = { A. Markowicz and T. Płociniczak and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-78049346290&partnerID=40&md5=cb3c70e41d46abdaedb116aeb4340f4c},

issn = {12301485},

year  = {2010},

date = {2010-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {19},

number = {5},

pages = {957-965},

abstract = {The effects of Cd, Ni, Cu, or Zn on the whole cell-derived fatty acid profiles of four bacterial strains isolated from heavy metal-polluted soils located in Upper Silesia was determined. Based on the fatty acid methyl ester (FAME) profiles, the strains were identified and named as Enterobacter intermedius AM15, Enterobacter intermedius MH8b, Pseudomonas putida MH1d, and Klebsiella pneumoniae AM12. The obtained results showed changes that were dependent both on tested strains and metal used. The most significant changes were observed for strains cultured in the Ni presence. In the FAME profiles of MH8b, AM 15, and AM12 strains, a significant increase of cyclopropane fatty acids was observed. Moreover, exposure for Ni resulted in the appearance of a new fatty acid in the FAME profiles of AM15 and MH8b strains. In turn, Cd and Zn caused a decrease of the content of cyclopropane fatty acids as compared to control. For AM15 and AM12 strains cultured on media with heavy metals, the ratio of saturated to unsaturated fatty acids were higher than that in control. The same phenomenon was also observed for MH8b strain exposed only to the highest concentration of Ni and Cd.},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}