• mgr Dorota Siwińska
Stanowisko: Kierownik biura
Jednostka: Biuro Ewaluacji i Obsługi Instytutów WNP
Adres: 40-032 Katowice, ul. Jagiellońska 28
Piętro: parter
Numer pokoju: B-12
Telefon: (32) 2009 592
E-mail: dorota.siwinska@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 22735286500
Publikacje z bazy Scopus
2018
Orzechowska, M.; Figura, K.; Siwińska, D.
Chromosomal distribution of rRNA genes in the karyotypes of two dioicous liverwort species from the genus Pellia Raddi Journal Article
In: Journal of Bryology, vol. 40, no. 4, pp. 384-392, 2018, ISSN: 03736687, (3).
@article{2-s2.0-85047930327,
title = {Chromosomal distribution of rRNA genes in the karyotypes of two dioicous liverwort species from the genus Pellia Raddi},
author = { M. Orzechowska and K. Figura and D. Siwińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047930327&doi=10.1080%2f03736687.2018.1474423&partnerID=40&md5=7c3dab07af5f051722db8eb4621a64eb},
doi = {10.1080/03736687.2018.1474423},
issn = {03736687},
year = {2018},
date = {2018-01-01},
journal = {Journal of Bryology},
volume = {40},
number = {4},
pages = {384-392},
publisher = {Taylor and Francis Ltd.},
abstract = {Over one hundred years have passed since the first cytogenetic studies were made on the liverwort genus Pellia Raddi. The karyotype of Pellia is characterised by large chromosomes, a varying heterochromatin content and the presence of sex chromosomes in the dioicous species. Most of the Pellia species are diploids with n = 9, but one of them, Pellia borealis Lorb., has been described as an example of allopolyploidy in liverworts. Although the localisation of rRNA genes, which are essential components of the nuclear genome, remains a challenge in bryophytes, data on the number and chromosomal localisation of 35S and 5S rDNA in all of the Pellia species are now available. Previously, fluorescence in situ hybridisation using rDNA probes was performed on the mitotic chromosomes of 2 monoicous species. The aim of this study was to establish the number and chromosomal distribution of rRNA genes in 2 dioicous diploid species—Pellia endiviifolia (Dicks.) Dumort. and Pellia neesiana (Gottsche) Limpr. The relationships between the species within the genus Pellia can now be discussed in the context of the localisation of the rDNA sites and the range in the number of rDNA loci among bryophytes can also be verified. © 2018, © British Bryological Society 2018.},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Over one hundred years have passed since the first cytogenetic studies were made on the liverwort genus Pellia Raddi. The karyotype of Pellia is characterised by large chromosomes, a varying heterochromatin content and the presence of sex chromosomes in the dioicous species. Most of the Pellia species are diploids with n = 9, but one of them, Pellia borealis Lorb., has been described as an example of allopolyploidy in liverworts. Although the localisation of rRNA genes, which are essential components of the nuclear genome, remains a challenge in bryophytes, data on the number and chromosomal localisation of 35S and 5S rDNA in all of the Pellia species are now available. Previously, fluorescence in situ hybridisation using rDNA probes was performed on the mitotic chromosomes of 2 monoicous species. The aim of this study was to establish the number and chromosomal distribution of rRNA genes in 2 dioicous diploid species—Pellia endiviifolia (Dicks.) Dumort. and Pellia neesiana (Gottsche) Limpr. The relationships between the species within the genus Pellia can now be discussed in the context of the localisation of the rDNA sites and the range in the number of rDNA loci among bryophytes can also be verified. © 2018, © British Bryological Society 2018.
2016
Orzechowska, M.; Gurdek, S.; Siwińska, D.; Piekarska-Stachowiak, A.
Cytogenetic characterization of the Arabidopsis thaliana natural tetraploid ecotype Warschau stability during in vitro regeneration Journal Article
In: Plant Cell, Tissue and Organ Culture, vol. 126, no. 3, pp. 553-560, 2016, ISSN: 01676857, (3).
@article{2-s2.0-84969134520,
title = {Cytogenetic characterization of the Arabidopsis thaliana natural tetraploid ecotype Warschau stability during in vitro regeneration},
author = { M. Orzechowska and S. Gurdek and D. Siwińska and A. Piekarska-Stachowiak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84969134520&doi=10.1007%2fs11240-016-1006-5&partnerID=40&md5=a6adece06a377085dcab6d107b964afb},
doi = {10.1007/s11240-016-1006-5},
issn = {01676857},
year = {2016},
date = {2016-01-01},
journal = {Plant Cell, Tissue and Organ Culture},
volume = {126},
number = {3},
pages = {553-560},
publisher = {Springer Netherlands},
abstract = {The morphological and cytogenetic features of the natural autotetraploid Arabidopsis thaliana ecotype Warschau (Wa-1) were investigated. Most of the Warschau plant organs that were analyzed showed higher size values in comparison with diploid Columbia plants. The tetraploid chromosome number was confirmed by analysis of mitotic metaphase cells and rDNA loci were localized. 35S rDNA loci were present on chromosomes 2 and 4, while 5S rDNA, which is polymorphic among A. thaliana ecotypes, were present on chromosomes 4 and 5. Well-characterized autotetraploid plant material was used for in vitro culture to investigate somaclonal variation. Efficient regeneration through organogenesis was achieved. Most of the plants obtained in vitro exhibited an unchanged ploidy level. Detailed cytogenetic analysis that included chromosome, chromocenters and rDNA signals numbers, revealed the stability of regenerants. Based on these data we recommend the ecotype Warschau as a well-characterized plant material for future investigations on the consequences of polyploidy for the genome. © 2016, The Author(s).},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The morphological and cytogenetic features of the natural autotetraploid Arabidopsis thaliana ecotype Warschau (Wa-1) were investigated. Most of the Warschau plant organs that were analyzed showed higher size values in comparison with diploid Columbia plants. The tetraploid chromosome number was confirmed by analysis of mitotic metaphase cells and rDNA loci were localized. 35S rDNA loci were present on chromosomes 2 and 4, while 5S rDNA, which is polymorphic among A. thaliana ecotypes, were present on chromosomes 4 and 5. Well-characterized autotetraploid plant material was used for in vitro culture to investigate somaclonal variation. Efficient regeneration through organogenesis was achieved. Most of the plants obtained in vitro exhibited an unchanged ploidy level. Detailed cytogenetic analysis that included chromosome, chromocenters and rDNA signals numbers, revealed the stability of regenerants. Based on these data we recommend the ecotype Warschau as a well-characterized plant material for future investigations on the consequences of polyploidy for the genome. © 2016, The Author(s).
Kolano, B. A.; McCann, J.; Orzechowska, M.; Siwińska, D.; Temsch, E.; Weiss-Schneeweiss, H.
Molecular and cytogenetic evidence for an allotetraploid origin of Chenopodium quinoa and C. berlandieri (Amaranthaceae) Journal Article
In: Molecular Phylogenetics and Evolution, vol. 100, pp. 109-123, 2016, ISSN: 10557903, (32).
@article{2-s2.0-84962915550,
title = {Molecular and cytogenetic evidence for an allotetraploid origin of Chenopodium quinoa and C. berlandieri (Amaranthaceae)},
author = { B.A. Kolano and J. McCann and M. Orzechowska and D. Siwińska and E. Temsch and H. Weiss-Schneeweiss},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84962915550&doi=10.1016%2fj.ympev.2016.04.009&partnerID=40&md5=f32cb4019dbada940d643557f2d49c7e},
doi = {10.1016/j.ympev.2016.04.009},
issn = {10557903},
year = {2016},
date = {2016-01-01},
journal = {Molecular Phylogenetics and Evolution},
volume = {100},
pages = {109-123},
publisher = {Academic Press Inc.},
abstract = {Most of the cultivated chenopods are polyploids, but their origin and evolutionary history are still poorly understood. Phylogenetic analyses of DNA sequences of four plastid regions, nrITS and nuclear 5S rDNA spacer region (NTS) of two tetraploid chenopods (2n = 4x = 36), Andean C. quinoa and North American C. berlandieri, and their diploid relatives allowed inferences of their origin. The phylogenetic analyses confirmed allotetraploid origin of both tetraploids involving diploids of two different genomic groups (genomes A and B) and suggested that these two might share very similar parentage.The hypotheses on the origin of the two allopolyploid species were further tested using genomic in situ hybridization (GISH). Several diploid Chenopodium species belonging to the two lineages, genome A and B, suggested by phylogenetic analyses, were tested as putative parental taxa. GISH differentiated two sets of parental chromosomes in both tetraploids and further corroborated their allotetraploid origin. Putative diploid parental taxa have been suggested by GISH for C. quinoa and C. berlandieri. Genome sizes of the analyzed allotetraploids fit nearly perfectly the expected additive values of the putative parental taxa. Directional and uniparental loss of rDNA loci of the maternal A-subgenome was revealed for both C. berlandieri and C. quinoa. © 2016 Elsevier Inc.},
note = {32},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Most of the cultivated chenopods are polyploids, but their origin and evolutionary history are still poorly understood. Phylogenetic analyses of DNA sequences of four plastid regions, nrITS and nuclear 5S rDNA spacer region (NTS) of two tetraploid chenopods (2n = 4x = 36), Andean C. quinoa and North American C. berlandieri, and their diploid relatives allowed inferences of their origin. The phylogenetic analyses confirmed allotetraploid origin of both tetraploids involving diploids of two different genomic groups (genomes A and B) and suggested that these two might share very similar parentage.The hypotheses on the origin of the two allopolyploid species were further tested using genomic in situ hybridization (GISH). Several diploid Chenopodium species belonging to the two lineages, genome A and B, suggested by phylogenetic analyses, were tested as putative parental taxa. GISH differentiated two sets of parental chromosomes in both tetraploids and further corroborated their allotetraploid origin. Putative diploid parental taxa have been suggested by GISH for C. quinoa and C. berlandieri. Genome sizes of the analyzed allotetraploids fit nearly perfectly the expected additive values of the putative parental taxa. Directional and uniparental loss of rDNA loci of the maternal A-subgenome was revealed for both C. berlandieri and C. quinoa. © 2016 Elsevier Inc.
2015
Kolano, B. A.; Siwińska, D.; McCann, J.; Weiss-Schneeweiss, H.
The evolution of genome size and rDNA in diploid species of Chenopodium s.l. (Amaranthaceae) Journal Article
In: Botanical Journal of the Linnean Society, vol. 179, no. 2, pp. 218-235, 2015, ISSN: 00244074, (20).
@article{2-s2.0-84941316011,
title = {The evolution of genome size and rDNA in diploid species of Chenopodium s.l. (Amaranthaceae)},
author = { B.A. Kolano and D. Siwińska and J. McCann and H. Weiss-Schneeweiss},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84941316011&doi=10.1111%2fboj.12321&partnerID=40&md5=e7855ebbd9a0efc76233b689ff8c7d98},
doi = {10.1111/boj.12321},
issn = {00244074},
year = {2015},
date = {2015-01-01},
journal = {Botanical Journal of the Linnean Society},
volume = {179},
number = {2},
pages = {218-235},
publisher = {Blackwell Publishing Ltd},
abstract = {The evolution of genome size and ribosomal DNA (rDNA) locus organization was analysed in 23 diploid species of Chenopodium s.l., all of which share the same base chromosome number of x=9. Phylogenetic relationships among these species were inferred from plastid and nuclear ribosomal internal transcribed spacer (nrITS) DNA sequences. The molecular phylogenetic analyses assigned all analysed species of Chenopodium s.l. to six evolutionary lineages, corresponding to the recent new generic taxonomic treatment of Chenopodium s.l. The distribution of rDNA loci for four species is presented here for the first time using fluorescence insitu hybridization (FISH) with 5S and 35S rDNA probes. Most of the 23 analysed diploid Chenopodium spp. possessed a single subterminally located 35S rDNA locus, except for three species which possessed two 35S rDNA loci. One or two 5S rDNA loci were typically localized subterminally on chromosomes, rarely interstitially. Analyses of rDNA locus numbers in a phylogenetic context resulted in the reconstruction of one locus each of 35S rDNA and 5S rDNA, both in subterminal positions, as the ancestral state. Genome sizes determined using flow cytometry were relatively small (2C value<2.8pg), ranging from 0.734pg in C.schraderianum to 2.721pg in C.californicum (nearly four-fold difference), and were often conserved within major phylogenetic lineages, suggesting an adaptive value. The reconstructed ancestral genome size was small for all evolutionary lineages, and changes have probably coincided with the divergence of major lineages. © 2015 The Linnean Society of London.},
note = {20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The evolution of genome size and ribosomal DNA (rDNA) locus organization was analysed in 23 diploid species of Chenopodium s.l., all of which share the same base chromosome number of x=9. Phylogenetic relationships among these species were inferred from plastid and nuclear ribosomal internal transcribed spacer (nrITS) DNA sequences. The molecular phylogenetic analyses assigned all analysed species of Chenopodium s.l. to six evolutionary lineages, corresponding to the recent new generic taxonomic treatment of Chenopodium s.l. The distribution of rDNA loci for four species is presented here for the first time using fluorescence insitu hybridization (FISH) with 5S and 35S rDNA probes. Most of the 23 analysed diploid Chenopodium spp. possessed a single subterminally located 35S rDNA locus, except for three species which possessed two 35S rDNA loci. One or two 5S rDNA loci were typically localized subterminally on chromosomes, rarely interstitially. Analyses of rDNA locus numbers in a phylogenetic context resulted in the reconstruction of one locus each of 35S rDNA and 5S rDNA, both in subterminal positions, as the ancestral state. Genome sizes determined using flow cytometry were relatively small (2C value<2.8pg), ranging from 0.734pg in C.schraderianum to 2.721pg in C.californicum (nearly four-fold difference), and were often conserved within major phylogenetic lineages, suggesting an adaptive value. The reconstructed ancestral genome size was small for all evolutionary lineages, and changes have probably coincided with the divergence of major lineages. © 2015 The Linnean Society of London.
2013
Orzechowska, M.; Stępień, K.; Kamińska, T.; Siwińska, D.
In: Plant Cell, Tissue and Organ Culture, vol. 112, no. 3, pp. 263-273, 2013, ISSN: 01676857, (14).
@article{2-s2.0-84874224743,
title = {Chromosome variations in regenerants of Arabidopsis thaliana derived from 2- and 6-week-old callus detected using flow cytometry and FISH analyses},
author = { M. Orzechowska and K. Stępień and T. Kamińska and D. Siwińska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874224743&doi=10.1007%2fs11240-012-0232-8&partnerID=40&md5=cafa333b53cba44958311eefc1f73a57},
doi = {10.1007/s11240-012-0232-8},
issn = {01676857},
year = {2013},
date = {2013-01-01},
journal = {Plant Cell, Tissue and Organ Culture},
volume = {112},
number = {3},
pages = {263-273},
publisher = {Kluwer Academic Publishers},
abstract = {Shoot organogenesis was induced from 2- and 6-week-old callus derived from the leaves of Arabidopsis thaliana ecotype Columbia (2n = 10). Regenerated plants were evaluated for chromosomal variations by means of flow cytometry and fluorescent in situ hybridization (FISH). Flow cytometric measurements revealed the occurrence of diploid, tetraploid, and octoploid plants among the regenerants of 2-week-old calli, whereas only diploid and tetraploid plants were regenerated from the 6-week-old calli. Chromosome counting showed that plants developed from the 2-week-old calli exhibited mixoploidy and a high frequency of aneuploid cells. These plants were infertile and displayed altered morphology. FISH with 5S and 25S rDNA probes allowed to detect some structural chromosomal rearrangements in regenerated plants. Along with cells which exhibited correct localisation of rDNA loci, also cells bearing chromosomal translocations, deletions or duplications were found. The type of structural aberrations varied between diploid and tetraploid regenerants. © 2012 The Author(s).},
note = {14},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Shoot organogenesis was induced from 2- and 6-week-old callus derived from the leaves of Arabidopsis thaliana ecotype Columbia (2n = 10). Regenerated plants were evaluated for chromosomal variations by means of flow cytometry and fluorescent in situ hybridization (FISH). Flow cytometric measurements revealed the occurrence of diploid, tetraploid, and octoploid plants among the regenerants of 2-week-old calli, whereas only diploid and tetraploid plants were regenerated from the 6-week-old calli. Chromosome counting showed that plants developed from the 2-week-old calli exhibited mixoploidy and a high frequency of aneuploid cells. These plants were infertile and displayed altered morphology. FISH with 5S and 25S rDNA probes allowed to detect some structural chromosomal rearrangements in regenerated plants. Along with cells which exhibited correct localisation of rDNA loci, also cells bearing chromosomal translocations, deletions or duplications were found. The type of structural aberrations varied between diploid and tetraploid regenerants. © 2012 The Author(s).
2012
Kwaśniewska, J.; Nawrocki, W.; Siwińska, D.; Małuszyńska, J.
DNA damage in Crepis capillaris cells in response to in vitro conditions Journal Article
In: Acta Biologica Cracoviensia Series Botanica, vol. 54, no. 2, pp. 93-101, 2012, ISSN: 00015296, (6).
@article{2-s2.0-84872443362,
title = {DNA damage in Crepis capillaris cells in response to in vitro conditions},
author = { J. Kwaśniewska and W. Nawrocki and D. Siwińska and J. Małuszyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872443362&doi=10.2478%2fv10182-012-0028-5&partnerID=40&md5=d13e45ab038169b246d72a88dcf948c4},
doi = {10.2478/v10182-012-0028-5},
issn = {00015296},
year = {2012},
date = {2012-01-01},
journal = {Acta Biologica Cracoviensia Series Botanica},
volume = {54},
number = {2},
pages = {93-101},
abstract = {We analyzed DNA damage, mitotic activity and polyploidization in Crepis capillaris callus cells during short- and long-term in vitro culture, and the influence of plant growth regulators on these processes. Changes in the concentration of growth regulators altered the stability of callus. The level of DNA damage was highly dependent on the growth regulator composition of the medium. Cytokinin at high concentrations damaged DNA in the absence of auxin. Short- and long-term callus differed in sensitivity to growth regulators. Mitotic activity changed when callus was transferred to medium with modified growth regulators. Callus cell nuclear DNA content increased with age and in response to plant growth regulators. Hormones played a role in the genetic changes in C. capillaries callus culture. We demonstrated the usefulness of C. capillaris callus culture as a model for analyzing the effect of culture conditions, including plant growth regulators, on genetic stability. © Polish Academy of Sciences and Jagiellonian University, Cracow 2012.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We analyzed DNA damage, mitotic activity and polyploidization in Crepis capillaris callus cells during short- and long-term in vitro culture, and the influence of plant growth regulators on these processes. Changes in the concentration of growth regulators altered the stability of callus. The level of DNA damage was highly dependent on the growth regulator composition of the medium. Cytokinin at high concentrations damaged DNA in the absence of auxin. Short- and long-term callus differed in sensitivity to growth regulators. Mitotic activity changed when callus was transferred to medium with modified growth regulators. Callus cell nuclear DNA content increased with age and in response to plant growth regulators. Hormones played a role in the genetic changes in C. capillaries callus culture. We demonstrated the usefulness of C. capillaris callus culture as a model for analyzing the effect of culture conditions, including plant growth regulators, on genetic stability. © Polish Academy of Sciences and Jagiellonian University, Cracow 2012.
Catalán, P.; Müller, J.; Hasterok, R.; Jenkins, G.; Mur, L. A. J.; Langdon, T.; Betekhtin, A.; Siwińska, D.; Pimentel, M.; López-Alvarez, D.
Evolution and taxonomic split of the model grass Brachypodium distachyon Journal Article
In: Annals of Botany, vol. 109, no. 2, pp. 385-405, 2012, ISSN: 03057364, (118).
@article{2-s2.0-84856757620,
title = {Evolution and taxonomic split of the model grass Brachypodium distachyon},
author = { P. Catalán and J. Müller and R. Hasterok and G. Jenkins and L.A.J. Mur and T. Langdon and A. Betekhtin and D. Siwińska and M. Pimentel and D. López-Alvarez},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84856757620&doi=10.1093%2faob%2fmcr294&partnerID=40&md5=9bd59148b57fadf44df0564a599ed16b},
doi = {10.1093/aob/mcr294},
issn = {03057364},
year = {2012},
date = {2012-01-01},
journal = {Annals of Botany},
volume = {109},
number = {2},
pages = {385-405},
abstract = {Background and Aims Brachypodium distachyon is being widely investigated across the world as a model plant for temperate cereals. This annual plant has three cytotypes (2n= 10; 20; 30) that are still regarded as part of a single species. Here, a multidisciplinary study has been conducted on a representative sampling of the three cytotypes to investigate their evolutionary relationships and origins, and to elucidate if they represent separate species. Methods Statistical analyses of 15 selected phenotypic traits were conducted in individuals from 36 lines or populations. Cytogenetic analyses were performed through flow cytometry, fluorescence in situ hybridization (FISH) with genomic (GISH) and multiple DNA sequences as probes, and comparative chromosome painting (CCP). Phylogenetic analyses were based on two plastid (ndhF; trnLF) and five nuclear (ITS; ETS; CAL; DGAT; GI) genes from different Brachypodium lineages, whose divergence times and evolutionary rates were estimated. Key Results The phenotypic analyses detected significant differences between the three cytotypes and demonstrated stability of characters in natural populations. Genome size estimations, GISH, FISH and CCP confirmed that the 2n= 10 and 2n= 20 cytotypes represent two different diploid taxa, whereas the 2n= 30 cytotype represents the allotetraploid derived from them. Phylogenetic analysis demonstrated that the 2n= 20 and 2n= 10 cytotypes emerged from two independent lineages that were, respectively, the maternal and paternal genome donors of the 2n= 30 cytotype. The 2n= 20 lineage was older and mutated significantly faster than the 2n= 10 lineage and all the core perennial Brachypodium species. Conclusions The substantial phenotypic, cytogenetic and molecular differences detected among the three B. distachyon sensu lato cytotypes are indicative of major speciation processes within this complex that allow their taxonomic separation into three distinct species. We have kept the name B. distachyon for the 2n= 10 cytotype and have described two novel species as B. stacei and B. hybridum for, respectively, the 2n= 20 and 2n= 30 cytotypes. © 2011 The Author.},
note = {118},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Background and Aims Brachypodium distachyon is being widely investigated across the world as a model plant for temperate cereals. This annual plant has three cytotypes (2n= 10; 20; 30) that are still regarded as part of a single species. Here, a multidisciplinary study has been conducted on a representative sampling of the three cytotypes to investigate their evolutionary relationships and origins, and to elucidate if they represent separate species. Methods Statistical analyses of 15 selected phenotypic traits were conducted in individuals from 36 lines or populations. Cytogenetic analyses were performed through flow cytometry, fluorescence in situ hybridization (FISH) with genomic (GISH) and multiple DNA sequences as probes, and comparative chromosome painting (CCP). Phylogenetic analyses were based on two plastid (ndhF; trnLF) and five nuclear (ITS; ETS; CAL; DGAT; GI) genes from different Brachypodium lineages, whose divergence times and evolutionary rates were estimated. Key Results The phenotypic analyses detected significant differences between the three cytotypes and demonstrated stability of characters in natural populations. Genome size estimations, GISH, FISH and CCP confirmed that the 2n= 10 and 2n= 20 cytotypes represent two different diploid taxa, whereas the 2n= 30 cytotype represents the allotetraploid derived from them. Phylogenetic analysis demonstrated that the 2n= 20 and 2n= 10 cytotypes emerged from two independent lineages that were, respectively, the maternal and paternal genome donors of the 2n= 30 cytotype. The 2n= 20 lineage was older and mutated significantly faster than the 2n= 10 lineage and all the core perennial Brachypodium species. Conclusions The substantial phenotypic, cytogenetic and molecular differences detected among the three B. distachyon sensu lato cytotypes are indicative of major speciation processes within this complex that allow their taxonomic separation into three distinct species. We have kept the name B. distachyon for the 2n= 10 cytotype and have described two novel species as B. stacei and B. hybridum for, respectively, the 2n= 20 and 2n= 30 cytotypes. © 2011 The Author.
Kolano, B. A.; Siwińska, D.; Gómez-Pando, L. R.; Szymanowska-Pułka, J.; Małuszyńska, J.
Genome size variation in Chenopodium quinoa (Chenopodiaceae) Journal Article
In: Plant Systematics and Evolution, vol. 298, no. 1, pp. 251-255, 2012, ISSN: 03782697, (18).
@article{2-s2.0-84855299568,
title = {Genome size variation in Chenopodium quinoa (Chenopodiaceae)},
author = { B.A. Kolano and D. Siwińska and L.R. Gómez-Pando and J. Szymanowska-Pułka and J. Małuszyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855299568&doi=10.1007%2fs00606-011-0534-z&partnerID=40&md5=e6c5bbd0876a371b2668bdae269a533d},
doi = {10.1007/s00606-011-0534-z},
issn = {03782697},
year = {2012},
date = {2012-01-01},
journal = {Plant Systematics and Evolution},
volume = {298},
number = {1},
pages = {251-255},
abstract = {The extent and significance of intraspecific genome size variation were analysed in quinoa (Chenopodium quinoa Willd.), a pseudocereal important for human consumption in the Andean region of South America. Flow cytometry, with propidium iodide as the DNA stain, was used to estimate the genome size of 20 quinoa accessions from Ecuador, Peru, Bolivia, Argentina, Chile and the USA. Limited genome size variation was found among the analysed accessions. The differences between the accessions were statistically significant but the maximum inter-accession difference between the populations with the largest and the smallest genome reached only 5.9%. The largest genome was found in population C4 from Chile (mean 3.077 pg/2C) and the smallest in the Peruvian population P2 (mean 2.905 pg/2C). The variation was not correlated with collection site; however, the quinoa accessions analysed in this study belonged to three distinct geographical groups: northern highland, southern highland and lowland. © 2011 The Author(s).},
note = {18},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The extent and significance of intraspecific genome size variation were analysed in quinoa (Chenopodium quinoa Willd.), a pseudocereal important for human consumption in the Andean region of South America. Flow cytometry, with propidium iodide as the DNA stain, was used to estimate the genome size of 20 quinoa accessions from Ecuador, Peru, Bolivia, Argentina, Chile and the USA. Limited genome size variation was found among the analysed accessions. The differences between the accessions were statistically significant but the maximum inter-accession difference between the populations with the largest and the smallest genome reached only 5.9%. The largest genome was found in population C4 from Chile (mean 3.077 pg/2C) and the smallest in the Peruvian population P2 (mean 2.905 pg/2C). The variation was not correlated with collection site; however, the quinoa accessions analysed in this study belonged to three distinct geographical groups: northern highland, southern highland and lowland. © 2011 The Author(s).
2011
Słomka, A.; Siwińska, D.; Wolny, E. A.; Kellner, K.; Kuta, E.
Influence of a heavy-metal-polluted environment on Viola tricolor genome size and chromosome number Journal Article
In: Acta Biologica Cracoviensia Series Botanica, vol. 53, no. 1, pp. 7-15, 2011, ISSN: 00015296, (9).
@article{2-s2.0-80053355540,
title = {Influence of a heavy-metal-polluted environment on Viola tricolor genome size and chromosome number},
author = { A. Słomka and D. Siwińska and E.A. Wolny and K. Kellner and E. Kuta},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80053355540&doi=10.2478%2fv10182-011-0001-8&partnerID=40&md5=58fedcffca784a953938a587fb28df9c},
doi = {10.2478/v10182-011-0001-8},
issn = {00015296},
year = {2011},
date = {2011-01-01},
journal = {Acta Biologica Cracoviensia Series Botanica},
volume = {53},
number = {1},
pages = {7-15},
abstract = {Intraspecific changes in genome size and chromosome number lead to divergence and species evolution. Heavy metals disturb the cell cycle and cause mutations. Areas contaminated by heavy metals (metalliferous sites) are places where microevolutionary processes accelerate; very often only a few generations are enough for a new genotype to arise. This study, which continues our long-term research on Viola tricolor (Violaceae), a species occurring on both metalliferous (Zn; Pb; Cd; Cu) and non-metalliferous soils in Western and Central Europe, is aimed at determining the influence of environments polluted with heavy metals on genome size and karyological variability. The genome size of V. tricolor ranged from 3.801 to 4.203 pg, but the differences between metallicolous and non-metallicolous populations were not statistically significant. Altered chromosome numbers were significantly more frequent in material from the polluted sites than from the non-polluted sites (43% versus 28%). Besides the standard chromosome number (2n = 26), aneuploid cells with lower (2n = 18-25) or higher (2n = 27; 28) chromosome numbers were found in plants from both types of site, but polyploid (2n = 42) cells were observed only in plants from the metalliferous locality. The lack of correlation between chromosome variability in root meristematic cells and genome size estimated from peduncle cells can be attributed to elimination of somatic mutations in generative meristem, producing chromosome-stable non-meristematic tissues in the peduncle. © Polish Academy of Sciences and Jagiellonian University, Cracow 2011.},
note = {9},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Intraspecific changes in genome size and chromosome number lead to divergence and species evolution. Heavy metals disturb the cell cycle and cause mutations. Areas contaminated by heavy metals (metalliferous sites) are places where microevolutionary processes accelerate; very often only a few generations are enough for a new genotype to arise. This study, which continues our long-term research on Viola tricolor (Violaceae), a species occurring on both metalliferous (Zn; Pb; Cd; Cu) and non-metalliferous soils in Western and Central Europe, is aimed at determining the influence of environments polluted with heavy metals on genome size and karyological variability. The genome size of V. tricolor ranged from 3.801 to 4.203 pg, but the differences between metallicolous and non-metallicolous populations were not statistically significant. Altered chromosome numbers were significantly more frequent in material from the polluted sites than from the non-polluted sites (43% versus 28%). Besides the standard chromosome number (2n = 26), aneuploid cells with lower (2n = 18-25) or higher (2n = 27; 28) chromosome numbers were found in plants from both types of site, but polyploid (2n = 42) cells were observed only in plants from the metalliferous locality. The lack of correlation between chromosome variability in root meristematic cells and genome size estimated from peduncle cells can be attributed to elimination of somatic mutations in generative meristem, producing chromosome-stable non-meristematic tissues in the peduncle. © Polish Academy of Sciences and Jagiellonian University, Cracow 2011.
2010
Orzechowska, M.; Siwińska, D.; Małuszyńska, J.
Molecular cytogenetic analyses of haploid and allopolyploid Pellia species Journal Article
In: Journal of Bryology, vol. 32, no. 2, pp. 113-121, 2010, ISSN: 03736687, (19).
@article{2-s2.0-77952941232,
title = {Molecular cytogenetic analyses of haploid and allopolyploid Pellia species},
author = { M. Orzechowska and D. Siwińska and J. Małuszyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952941232&doi=10.1179%2f037366810X12578498136075&partnerID=40&md5=c38d72c6079a3105efc2ddc31c956d07},
doi = {10.1179/037366810X12578498136075},
issn = {03736687},
year = {2010},
date = {2010-01-01},
journal = {Journal of Bryology},
volume = {32},
number = {2},
pages = {113-121},
abstract = {The allopolyploid origin of several bryophyte species was reported approximately 20 years ago. Among them the best characterized is Pellia borealis, a hybrid of two cryptic sibling species: Pellia epiphylla-species N and P. epiphylla-species S. Genomes of the allopolyploid liverwort P. borealis and its progenitors were investigated using conventional as well as molecular cytogenetic techniques. The nuclear DNA content and cell cycle phase in the thallus nuclei was established using flow cytometry. Fluorescent differential staining, C-banding and fluorescent in situ hybridization (FISH) with 26S and 5S ribosomal DNA (rDNA) probes revealed new features of the chromosomes in the P. epiphylla-P. borealis complex. Some characteristics found in both the polyploid and haploid karyotypes support earlier suggestions about the allopolyploid origin of P. borealis. The banding pattern observed on P. borealis chromosomes suggests the occurrence of structural changes in the allopolyploid genome. The number and localization of rDNA sequences were established and simultaneous FISH with 26S and 5S rDNA probes showed colocalization of both types of ribosomal RNA (rRNA) genes in Pellia chromosomes. A nuclear DNA estimate showed that the P. borealis nuclear DNA content is about twice that of the largest known nuclear genome previously known in bryophytes. © 2010 British Bryological Society.},
note = {19},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The allopolyploid origin of several bryophyte species was reported approximately 20 years ago. Among them the best characterized is Pellia borealis, a hybrid of two cryptic sibling species: Pellia epiphylla-species N and P. epiphylla-species S. Genomes of the allopolyploid liverwort P. borealis and its progenitors were investigated using conventional as well as molecular cytogenetic techniques. The nuclear DNA content and cell cycle phase in the thallus nuclei was established using flow cytometry. Fluorescent differential staining, C-banding and fluorescent in situ hybridization (FISH) with 26S and 5S ribosomal DNA (rDNA) probes revealed new features of the chromosomes in the P. epiphylla-P. borealis complex. Some characteristics found in both the polyploid and haploid karyotypes support earlier suggestions about the allopolyploid origin of P. borealis. The banding pattern observed on P. borealis chromosomes suggests the occurrence of structural changes in the allopolyploid genome. The number and localization of rDNA sequences were established and simultaneous FISH with 26S and 5S rDNA probes showed colocalization of both types of ribosomal RNA (rRNA) genes in Pellia chromosomes. A nuclear DNA estimate showed that the P. borealis nuclear DNA content is about twice that of the largest known nuclear genome previously known in bryophytes. © 2010 British Bryological Society.
2009
Kolano, B. A.; Siwińska, D.; Małuszyńska, J.
Endopolyploidy patterns during development of Chenopodium quinoa Journal Article
In: Acta Biologica Cracoviensia Series Botanica, vol. 51, no. 2, pp. 85-92, 2009, ISSN: 00015296, (21).
@article{2-s2.0-77951718925,
title = {Endopolyploidy patterns during development of Chenopodium quinoa},
author = { B.A. Kolano and D. Siwińska and J. Małuszyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951718925&partnerID=40&md5=c10142ddd34a96ba8ccc6acfe02422ee},
issn = {00015296},
year = {2009},
date = {2009-01-01},
journal = {Acta Biologica Cracoviensia Series Botanica},
volume = {51},
number = {2},
pages = {85-92},
abstract = {Patterns of endopolyploidy were studied in embryos and seedlings during early development. Relative nuclear DNA content was measured with DAPI staining and flow cytometry. Somatic tissue of Chenopodium quinoa (Chenopodiaceae) revealed extensive endopolyploidization; tissues comprised mixtures of cells with DNA content ranging from 2C to 16C in varying proportions. Endopolyploidy patterns corresponded to the developmental stage and the individual organ. Polysomaty was already present in the radicle of the embryo in the imbibited seed. During seedling development, endopolyploidization took place in many examined organs (roots; hypocotyls; cotyledons) to different extents. The C-value was highest in the differentiated root, where up to 50% of the cell underwent one or two endocycles. Endopolyploidization was not present in nuclei from leaves and the shoot apex. © Polish Academy of Sciences and Jaglellonlan University.},
note = {21},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Patterns of endopolyploidy were studied in embryos and seedlings during early development. Relative nuclear DNA content was measured with DAPI staining and flow cytometry. Somatic tissue of Chenopodium quinoa (Chenopodiaceae) revealed extensive endopolyploidization; tissues comprised mixtures of cells with DNA content ranging from 2C to 16C in varying proportions. Endopolyploidy patterns corresponded to the developmental stage and the individual organ. Polysomaty was already present in the radicle of the embryo in the imbibited seed. During seedling development, endopolyploidization took place in many examined organs (roots; hypocotyls; cotyledons) to different extents. The C-value was highest in the differentiated root, where up to 50% of the cell underwent one or two endocycles. Endopolyploidization was not present in nuclei from leaves and the shoot apex. © Polish Academy of Sciences and Jaglellonlan University.
Dydak, M.; Kolano, B. A.; Nowak, T.; Siwińska, D.; Małuszyńska, J.
Cytogenetic studies of three European species of Centaurea L. (Asteraceae) Journal Article
In: Hereditas, vol. 146, no. 4, pp. 152-161, 2009, ISSN: 00180661, (27).
@article{2-s2.0-70349507009,
title = {Cytogenetic studies of three European species of Centaurea L. (Asteraceae)},
author = { M. Dydak and B.A. Kolano and T. Nowak and D. Siwińska and J. Małuszyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349507009&doi=10.1111%2fj.1601-5223.2009.02113.x&partnerID=40&md5=c0b00cf8092fcd68ff1b5bfae8a49d25},
doi = {10.1111/j.1601-5223.2009.02113.x},
issn = {00180661},
year = {2009},
date = {2009-01-01},
journal = {Hereditas},
volume = {146},
number = {4},
pages = {152-161},
abstract = {Cytogenetic analysis of several populations of Centaurea jacea (2n = 4x = 44), C. oxylepis (2n = 4x = 44) and C. phrygia (2n = 2x = 22) was performed using flow cytometry, differential chromosome staining and FISH. In all species Arabidopsis-type telomeric repeats hybridized only to the terminal part of chromosomes. In C. phrygia three pairs and in C. oxylepis six pairs of chromosomes revealed the hybridization signals of 45S rDNA. Centaurea jacea showed polymorphism in the 45S rDNA loci number, five or six pairs of sites were observed. 5S rDNA loci were located in two pairs of chromosomes in C. phrygia. In C. jacea and C. oxylepis the number and position of 5S rDNA loci were the same: three pairs located interstitially and one terminally. The genome size of the diploid C. phrygia was established as 2.14 pg/2C. The genomes of tetraploid species were nearly two times larger and genome size polymorphism was observed among C. jacea populations. © 2009 The Authors.},
note = {27},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Cytogenetic analysis of several populations of Centaurea jacea (2n = 4x = 44), C. oxylepis (2n = 4x = 44) and C. phrygia (2n = 2x = 22) was performed using flow cytometry, differential chromosome staining and FISH. In all species Arabidopsis-type telomeric repeats hybridized only to the terminal part of chromosomes. In C. phrygia three pairs and in C. oxylepis six pairs of chromosomes revealed the hybridization signals of 45S rDNA. Centaurea jacea showed polymorphism in the 45S rDNA loci number, five or six pairs of sites were observed. 5S rDNA loci were located in two pairs of chromosomes in C. phrygia. In C. jacea and C. oxylepis the number and position of 5S rDNA loci were the same: three pairs located interstitially and one terminally. The genome size of the diploid C. phrygia was established as 2.14 pg/2C. The genomes of tetraploid species were nearly two times larger and genome size polymorphism was observed among C. jacea populations. © 2009 The Authors.
2008
Kolano, B. A.; Siwińska, D.; Małuszyńska, J.
Comparative cytogenetic analysis of diploid and hexaploid Chenopodium album Agg. Journal Article
In: Acta Societatis Botanicorum Poloniae, vol. 77, no. 4, pp. 293-298, 2008, ISSN: 00016977, (13).
@article{2-s2.0-60049084223,
title = {Comparative cytogenetic analysis of diploid and hexaploid Chenopodium album Agg.},
author = { B.A. Kolano and D. Siwińska and J. Małuszyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-60049084223&partnerID=40&md5=db8120a0063b8d8b1f0f429aac9fecdd},
issn = {00016977},
year = {2008},
date = {2008-01-01},
journal = {Acta Societatis Botanicorum Poloniae},
volume = {77},
number = {4},
pages = {293-298},
publisher = {Polish Botanical Society},
abstract = {Two cytotypes of Chenopodium album, diploid (2n=2x=18) and hexaploid (2n=6x=54), were analysed using flow cytometry and a FISH experiment. The genome size was indicated as 1.795 pg for the diploid and 3.845 pg for the hexaploid plants which suggested genome downsizing in the evolution of hexaploid cytotype. Double FISH with 25S rDNA and 5S rDNA allowed three to five homologue chromosome pairs to be distinguished depending on the cytotype. The variation in size and number of rDNA sites between the polyploid C. album and its putative diploid ancestor indicated that rDNA loci underwent rearrangements after polyploidization. Flow cytometry measurements of the relative nuclear DNA content in the somatic tissue of C. album revealed extensive endopolyploidization resulting in tissues comprising a mixture of cells with a different DNA content (from 2C to 32C) in varying proportions. The pattern of endopolyploidy was characteristic for the developmental stage of the plant and for the individual organ. Polysomaty was not observed in the embryo tissues however endopolyploidization had taken place in most tested organs of seedlings. The endopolyploidy in diploid and hexaploid C. album was compared to find any relationship between the pattern of polysomaty and polyploidy level in this species. This revealed that polyploid plants showed a decline in the number of endocycles as well as in the frequency of endopolyploidy cells compared to diploid plants.},
note = {13},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Two cytotypes of Chenopodium album, diploid (2n=2x=18) and hexaploid (2n=6x=54), were analysed using flow cytometry and a FISH experiment. The genome size was indicated as 1.795 pg for the diploid and 3.845 pg for the hexaploid plants which suggested genome downsizing in the evolution of hexaploid cytotype. Double FISH with 25S rDNA and 5S rDNA allowed three to five homologue chromosome pairs to be distinguished depending on the cytotype. The variation in size and number of rDNA sites between the polyploid C. album and its putative diploid ancestor indicated that rDNA loci underwent rearrangements after polyploidization. Flow cytometry measurements of the relative nuclear DNA content in the somatic tissue of C. album revealed extensive endopolyploidization resulting in tissues comprising a mixture of cells with a different DNA content (from 2C to 32C) in varying proportions. The pattern of endopolyploidy was characteristic for the developmental stage of the plant and for the individual organ. Polysomaty was not observed in the embryo tissues however endopolyploidization had taken place in most tested organs of seedlings. The endopolyploidy in diploid and hexaploid C. album was compared to find any relationship between the pattern of polysomaty and polyploidy level in this species. This revealed that polyploid plants showed a decline in the number of endocycles as well as in the frequency of endopolyploidy cells compared to diploid plants.
2007
Fras, A.; Kwaśniewska, J.; Siwińska, D.; Małuszyńska, J.
Cytological events in explants of Arabidopsis thaliana during early callogenesis Journal Article
In: Plant Cell Reports, vol. 26, no. 11, pp. 1933-1939, 2007, ISSN: 07217714, (11).
@article{2-s2.0-35248883873,
title = {Cytological events in explants of Arabidopsis thaliana during early callogenesis},
author = { A. Fras and J. Kwaśniewska and D. Siwińska and J. Małuszyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-35248883873&doi=10.1007%2fs00299-007-0415-7&partnerID=40&md5=5b34138958d67617b682da67c7d00883},
doi = {10.1007/s00299-007-0415-7},
issn = {07217714},
year = {2007},
date = {2007-01-01},
journal = {Plant Cell Reports},
volume = {26},
number = {11},
pages = {1933-1939},
abstract = {Leaf explants of diploid (2n = 2x = 10) and autotetraploid (2n = 4x = 20) plants of Arabidopsis thaliana ecotype Columbia were cytologically and cytogenetically analysed to determine the time and the mechanisms of the process of polyploidization. The first polyploid cells were observed after the third day of culture in both genotypes of explants. Polyploid cells were the result of pre-existing mixoploidy in explants of A. thaliana. Other factors such as endoreduplication, endomitosis, abnormal microtubules arrangement and DNA damage may have induced polyploidization during early stages of callogenesis. © 2007 Springer-Verlag.},
note = {11},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Leaf explants of diploid (2n = 2x = 10) and autotetraploid (2n = 4x = 20) plants of Arabidopsis thaliana ecotype Columbia were cytologically and cytogenetically analysed to determine the time and the mechanisms of the process of polyploidization. The first polyploid cells were observed after the third day of culture in both genotypes of explants. Polyploid cells were the result of pre-existing mixoploidy in explants of A. thaliana. Other factors such as endoreduplication, endomitosis, abnormal microtubules arrangement and DNA damage may have induced polyploidization during early stages of callogenesis. © 2007 Springer-Verlag.
2005
Góralski, G.; Popielarska, M.; Ślesak, H.; Siwińska, D.; Batycka, M.
Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro Journal Article
In: Acta Biologica Cracoviensia Series Botanica, vol. 47, no. 2, pp. 121-128, 2005, ISSN: 00015296, (31).
@article{2-s2.0-33644534545,
title = {Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro},
author = { G. Góralski and M. Popielarska and H. Ślesak and D. Siwińska and M. Batycka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644534545&partnerID=40&md5=3ff3c37f21b52a025ca0472b2b99bf9b},
issn = {00015296},
year = {2005},
date = {2005-01-01},
journal = {Acta Biologica Cracoviensia Series Botanica},
volume = {47},
number = {2},
pages = {121-128},
abstract = {An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2;4-D; TDZ; IAA; NAA; BAP; kinetin; 2iP) for regeneration. There were significant differences in regeneration response between medium supplemented with TDZ and medium with other hormones. Only medium containing TDZ stimulated shoot induction. The highest efficiency of shoot regeneration (avg. 6.2 shoots per culture) was on medium supplemented with 0.5 mg/l TDZ. The ploidy of callus and organs formed in endosperm culture was examined by flow cytometry. The results, peaks corresponding to 3C DNA amounts, confirmed the endospermal origin of callus, roots and shoots. Aneuploid and polyploid cells were found in endosperm-derived callus and regenerated organs. © Polish Academy of Sciences, Cracow 2005.},
note = {31},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2;4-D; TDZ; IAA; NAA; BAP; kinetin; 2iP) for regeneration. There were significant differences in regeneration response between medium supplemented with TDZ and medium with other hormones. Only medium containing TDZ stimulated shoot induction. The highest efficiency of shoot regeneration (avg. 6.2 shoots per culture) was on medium supplemented with 0.5 mg/l TDZ. The ploidy of callus and organs formed in endosperm culture was examined by flow cytometry. The results, peaks corresponding to 3C DNA amounts, confirmed the endospermal origin of callus, roots and shoots. Aneuploid and polyploid cells were found in endosperm-derived callus and regenerated organs. © Polish Academy of Sciences, Cracow 2005.
2003
Hajdera, I.; Siwińska, D.; Hasterok, R.; Małuszyńska, J.
Molecular cytogenetic analysis of genome structure in Lupinus angustifolius and Lupinus cosentinii Journal Article
In: Theoretical and Applied Genetics, vol. 107, no. 6, pp. 988-996, 2003, ISSN: 00405752, (55).
@article{2-s2.0-0142028915,
title = {Molecular cytogenetic analysis of genome structure in Lupinus angustifolius and Lupinus cosentinii},
author = { I. Hajdera and D. Siwińska and R. Hasterok and J. Małuszyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0142028915&doi=10.1007%2fs00122-003-1303-3&partnerID=40&md5=5a3f91ea2499ae60339d064216c56cbb},
doi = {10.1007/s00122-003-1303-3},
issn = {00405752},
year = {2003},
date = {2003-01-01},
journal = {Theoretical and Applied Genetics},
volume = {107},
number = {6},
pages = {988-996},
abstract = {Molecular cytogenetic analysis of Lupinus angustifolius and Lupinus cosentinii was performed using flow cytometry, fluorescence in situ hybridisation (FISH) and differential chromosome staining. Genome size was determined as 2.07 pg for L. angustifolius and 1.54 pg for L. cosentinii. Analysis of nuclear DNA amount in cells during plant development has shown endopolyploidisation in different organs. The highest level of endopolyploidy was in cotyledons and reached 32C in L. angustifolius and 64C in L. cosentinii. Both of the investigated Lupinus species belong to the polysomatic type of plants. Double FISH with rDNA probes provided chromosomal landmarks for 10 out of 40 chromosomes for L. angustifolius and 8 out of 32 chromosomes for L. cosentinii. In L. angustifolius, the number and localisation of 25S rDNA hybridisation signals precisely corresponded to the chromomycin A3 (CMA +) bands, while in L. cosentinii both 25S and 5S rDNA loci overlapped with CMA+ bands. Silver staining revealed that only 45S rRNA genes located in secondary constriction regions were transcriptionally active. FISH with Arabidopsis-type telomeric arrays revealed the presence of signals at termini of all chromosomes. Despite the application of different DNA probes for FISH and different chromosome staining, a relatively small proportion of chromosomes in the Lupinus karyotypes can be distinguished. Identification of all chromosomes requires the use of more chromosome-specific markers.},
note = {55},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Molecular cytogenetic analysis of Lupinus angustifolius and Lupinus cosentinii was performed using flow cytometry, fluorescence in situ hybridisation (FISH) and differential chromosome staining. Genome size was determined as 2.07 pg for L. angustifolius and 1.54 pg for L. cosentinii. Analysis of nuclear DNA amount in cells during plant development has shown endopolyploidisation in different organs. The highest level of endopolyploidy was in cotyledons and reached 32C in L. angustifolius and 64C in L. cosentinii. Both of the investigated Lupinus species belong to the polysomatic type of plants. Double FISH with rDNA probes provided chromosomal landmarks for 10 out of 40 chromosomes for L. angustifolius and 8 out of 32 chromosomes for L. cosentinii. In L. angustifolius, the number and localisation of 25S rDNA hybridisation signals precisely corresponded to the chromomycin A3 (CMA +) bands, while in L. cosentinii both 25S and 5S rDNA loci overlapped with CMA+ bands. Silver staining revealed that only 45S rRNA genes located in secondary constriction regions were transcriptionally active. FISH with Arabidopsis-type telomeric arrays revealed the presence of signals at termini of all chromosomes. Despite the application of different DNA probes for FISH and different chromosome staining, a relatively small proportion of chromosomes in the Lupinus karyotypes can be distinguished. Identification of all chromosomes requires the use of more chromosome-specific markers.