@article{2-s2.0-85128842423,

title = {Ovaries and testes of Lithobius forficatus (Myriapoda, Chilopoda) react differently to the presence of cadmium in the environment},

author = { I. Poprawa and Ł. Chajec and A. Chachulska-Żymełka and G. Wilczek and S. Student and M. Leśniewska and M.M. Rost-Roszkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128842423&doi=10.1038%2fs41598-022-10664-4&partnerID=40&md5=8b63d068ae6726dff9844ca0d4a93be1},

doi = {10.1038/s41598-022-10664-4},

issn = {20452322},

year  = {2022},

date = {2022-01-01},

journal = {Scientific Reports},

volume = {12},

number = {1},

publisher = {Nature Research},

abstract = {Proper reproduction depends on properly functioning gonads (ovaries and testes). Many xenobiotics, including heavy metals, can cause changes in somatic and germ line cells, thus damaging the reproductive capacity. The aim of this study was to investigate the effect of the heavy metal cadmium on the gonads, including germ line and somatic cells. It is important to determine whether cell death processes are triggered in both types of cells in the gonads, and which gonads are more sensitive to the presence of cadmium in the environment. The research was conducted on the soil-dwelling arthropod Lithobius forficatus (Myriapoda; Chilopoda), which is common for European fauna. Animals were cultured in soil supplemented with Cd for different periods (short- and long-term treatment). Gonads were isolated and prepared for qualitative and quantitative analysis, which enabled us to describe all changes which appeared after both the short- and long-term cadmium treatment. The results of our study showed that cadmium affects the structure and ultrastructure of both gonads in soil-dwelling organisms including the activation of cell death processes. However, the male germ line cells are more sensitive to cadmium than female germ line cells. We also observed that germ line cells are protected by the somatic cells of both gonads. © 2022, The Author(s).},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85134226759,

title = {Hazards related to the presence of cadmium in food – Studies on the European soil centipede, Lithobius forficatus},

author = { M.M. Rost-Roszkowska and I. Poprawa and Ł. Chajec and A. Chachulska-Żymełka and G. Wilczek and M. Skowronek and S. Student and M. Leśniewska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85134226759&doi=10.1016%2fj.scitotenv.2022.157298&partnerID=40&md5=3e44db2c8124fdeab23b618b4495900f},

doi = {10.1016/j.scitotenv.2022.157298},

issn = {00489697},

year  = {2022},

date = {2022-01-01},

journal = {Science of the Total Environment},

volume = {845},

publisher = {Elsevier B.V.},

abstract = {The soil is an environment rich in numerous potentially toxic substances/elements when present at elevated concentrations. They can be transported through the successive levels of the trophic chain. Animals living in a contaminated environment or eating contaminated food can accumulate potentially toxic elements in their bodies. One of the potentially toxic metals is cadmium, which accumulates significantly in soils. The aim of our research was to evaluate the changes caused by cadmium supplied with the food administered to invertebrates living in uncontaminated soil. The results were compared with those obtained for animals raised in contaminated soil, where cadmium entered the body via the epidermis. As the material for studies, we chose a common European soil centipede, Lithobius forficatus. Adult specimens were divided into the following experimental groups: C – control animals, Cd12 and Cd45 – animals fed with Chironomus larvae maintained in water containing 80 mg/l CdCl2, for 12 and 45 days, respectively. The material was analyzed using qualitative and quantitative analysis (transmission electron microscopy; confocal microscopy; flow cytometry; atomic absorption spectrometry). Eventually, we can conclude that the digestive system is an effective barrier against the effects of toxic metals on the entire organism, but among the gonads, ovaries are more protected than testes, however, this protection is not sufficient. Accumulation of spherites and mitochondrial alterations are probably involved in survival mechanisms of tissues after Cd intoxication. © 2022},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85105631786,

title = {Effects of cadmium on mitochondrial structure and function in different organs: studies on the soil centipede Lithobius forficatus (Myriapoda, Chilopoda)},

author = { M.M. Rost-Roszkowska and I. Poprawa and Ł. Chajec and A. Chachulska-Żymełka and G. Wilczek and P. Wilczek and M. Tarnawska and S. Student and M. Leśniewska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105631786&doi=10.1080%2f24750263.2021.1912199&partnerID=40&md5=521cb3648cd8129014a1058fea253b44},

doi = {10.1080/24750263.2021.1912199},

issn = {24750263},

year  = {2021},

date = {2021-01-01},

journal = {European Zoological Journal},

volume = {88},

number = {1},

pages = {632-648},

publisher = {Taylor and Francis Ltd.},

abstract = {Mitochondria are organelles that play a crucial role in cell physiology, cell death, and aging. They are among the first responders to different stressors that originate from the environment. Cadmium as a heavy metal affects different levels of body organization: from organs through tissues and cells to organelles. Based on our previous research results, we decided to check how the exposure to cadmium affects the functioning of mitochondria in different organs of soil living centipede Lithobius forficatus. The activity of mitochondria in somatic and germ cells has been analyzed using transmission electron microscopy (TEM), confocal microscopy, and flow cytometry. Changes in the mitochondrial membrane potential and mitochondrial dismutase (MnSOD) activity in relation to the accumulation of reactive oxygen species (ROS) caused by cadmium exposure have been studied. Individuals were divided into 3 experimental groups depending on cadmium concentration in soil. Changes in mitochondrial ultrastructure caused by cadmium are tissue-dependent and associated with an increase of ROS levels. The system of ROS and MnSOD activation works more efficiently in the case of gonads than in the digestive system. While the short-term cadmium exposure alters the fine structure of both the somatic and germ-line cells in gonads, the long-term cadmium exposure causes mitochondrial ultrastructure regeneration. © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85087489337,

title = {Effects of short- and long-term exposure to cadmium on salivary glands and fat body of soil centipede Lithobius forficatus (Myriapoda, Chilopoda): Histology and ultrastructure},

author = { M.M. Rost-Roszkowska and I. Poprawa and Ł. Chajec and A. Chachulska-Żymełka and M. Leśniewska and S. Student},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087489337&doi=10.1016%2fj.micron.2020.102915&partnerID=40&md5=6309db2f521f14d140f64b2f7000a299},

doi = {10.1016/j.micron.2020.102915},

issn = {09684328},

year  = {2020},

date = {2020-01-01},

journal = {Micron},

volume = {137},

publisher = {Elsevier Ltd},

abstract = {Cadmium (Cd) is the most widely studied heavy metal in terms of food-chain accumulation and contamination because it can strongly affect all environments (e.g.; soil; water; air). It can accumulate in different tissues and organs and can affect the organism at different levels of organization: from organs, tissues and cells though cell organelles and structures to activation of mechanisms of survival and cell death. In soil-dwelling organisms heavy metals gather in all tissues with accumulation properties: midgut, salivary glands, fat body. The aim of this study was to describe the effects of cadmium on the soil species Lithobius forficatus, mainly on two organs responsible for gathering different substances, the fat body and salivary glands, at the ultrastructural level. Changes caused by cadmium short- and long-term intoxication, connected with cell death (autophagy; apoptosis; necrosis), and the crosstalk between them, were analyzed. Adult specimens of L. forficatus were collected in a natural environment and divided into three experimental groups: C (the control group), Cd1 (cultured in soil with 80 mg/kg of CdCl2 for 12 days) and Cd2 (cultured in soil with 80 mg/kg of CdCl2 for 45 days). Transmission electron microscopy revealed ultrastructural alterations in both of the organs analyzed (reduction in the amount of reserve material; the appearance of vacuoles; etc.). Qualitative analysis using TUNEL assay revealed distinct crosstalk between autophagy and necrosis in the fat body adipocytes, while crosstalk between autophagy, apoptosis and necrosis in the salivary glands was detected in salivary glands of the centipedes examined here. We conclude that different organs in the body can react differently to the same stressor, as well as to the same concentration and time of exposure. Different mechanisms at the ultrastructural level activate different types of cell death and with different dynamics. © 2020 Elsevier Ltd},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85086015206,

title = {Influence of soil contaminated with cadmium on cell death in the digestive epithelium of soil centipede Lithobius forficatus (Myriapoda, Chilopoda)},

author = { M.M. Rost-Roszkowska and I. Poprawa and Ł. Chajec and A. Chachulska-Żymełka and G. Wilczek and P. Wilczek and S. Student and M. Skowronek and A. Nadgórska-Socha and M. Leśniewska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086015206&doi=10.1080%2f24750263.2020.1757168&partnerID=40&md5=58f49682d112d8cec5ea24fe0e5c2b11},

doi = {10.1080/24750263.2020.1757168},

issn = {24750263},

year  = {2020},

date = {2020-01-01},

journal = {European Zoological Journal},

volume = {87},

number = {1},

pages = {242-262},

publisher = {Taylor and Francis Ltd.},

abstract = {Cadmium is a heavy metal that is treated as an environmental pollutant (air; water; soil). In order to understand the potential effects of cadmium in soil and soil invertebrates, it is important to describe all alterations which appear at different levels in organisms. The main aim of this study was to investigate, analyze and describe the alterations caused by cadmium short- and long-term intoxication at different levels in the organisms: from tissues to cells and organelles. In addition, the activation of cell death mechanisms that take part in homeostasis maintenance according to cadmium has been studied. Therefore, as the species for this project, a terrestrial and well-known widespread European species–the centipede Lithobius forficatus (Myriapoda; Chilopoda; Lithobiomorpha)–was chosen. This omnivorous species lives under upper layers of soil, under stones, litter, rocks, and leaves, and it is also commonly found in human habitats. The animals were divided into three groups: C–the control group, animals cultured in a horticultural soil; Cd1–animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, 12 days–short-term exposure; Cd2–animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, 45 days–long-term exposure. The midgut was isolated from each specimen and it was prepared for analysis using some histological, histochemical and immunohistochemical methods. Our studies showed that short-term intoxication causes intensification of autophagy and digestion of reserve material, while long-term exposure to this heavy metal causes activation of cell death processes together with inhibition of autophagy connected with the lack of reserve material. Additionally, we can infer that autophagy and cell death are nutrient deprivation-induced processes. Finally, we can conclude that short- and long-term exposure of soil centipede to cadmium affects different mechanisms and processes of cell death. © 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85065912638,

title = {Autophagy and apoptosis in the midgut epithelium of millipedes},

author = { M.M. Rost-Roszkowska and J. Vilimová and K. Tajovský and A. Chachulska-Żymełka and A. Sosinka and M. Kszuk-Jendrysik and A. Ostróżka and F. Kaszuba},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065912638&doi=10.1017%2fS143192761900059X&partnerID=40&md5=c61361bfea2cb26d1ebf674d1ed0a10d},

doi = {10.1017/S143192761900059X},

issn = {14319276},

year  = {2019},

date = {2019-01-01},

journal = {Microscopy and Microanalysis},

volume = {25},

number = {4},

pages = {1004-1016},

publisher = {Cambridge University Press},

abstract = {The process of autophagy has been detected in the midgut epithelium of four millipede species: Julus scandinavius, Polyxenus lagurus, Archispirostreptus gigas, and Telodeinopus aoutii. It has been examined using transmission electron microscopy (TEM), which enabled differentiation of cells in the midgut epithelium, and some histochemical methods (light microscope and fluorescence microscope). While autophagy appeared in the cytoplasm of digestive, secretory, and regenerative cells in J. scandinavius and A. gigas, in the two other species, T. aoutii and P. lagurus, it was only detected in the digestive cells. Both types of macroautophagy, the selective and nonselective processes, are described using TEM. Phagophore formation appeared as the first step of autophagy. After its blind ends fusion, the autophagosomes were formed. The autophagosomes fused with lysosomes and were transformed into autolysosomes. As the final step of autophagy, the residual bodies were detected. Autophagic structures can be removed from the midgut epithelium via, e.g., atypical exocytosis. Additionally, in P. lagurus and J. scandinavius, it was observed as the neutralization of pathogens such as Rickettsia-like microorganisms. Autophagy and apoptosis ca be analyzed using TEM, while specific histochemical methods may confirm it. © Microscopy Society of America 2019.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77956465521,

title = {Apoptosis and autophagy in the midgut epithelium of Acheta domesticus (Insecta, Orthoptera, Gryllidae)},

author = { M.M. Rost-Roszkowska and I. Poprawa and A. Chachulska-Żymełka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956465521&doi=10.2108%2fzsj.27.740&partnerID=40&md5=fabe9e7f3c40eb1eb7a363ad398cacef},

doi = {10.2108/zsj.27.740},

issn = {02890003},

year  = {2010},

date = {2010-01-01},

journal = {Zoological Science},

volume = {27},

number = {9},

pages = {740-745},

abstract = {The midgut epithelium of Acheta domesticus (Insecta; Orthoptera; Gryllidae), which is composed of columnar digestive cells and regenerative crypts, degenerates in two manners: necrotic and apoptotic. While necrosis was described in our previous paper, programmed cell death was the aim of the present studies. The first morphological signs of programmed cell death in midgut epithelium cells are alterations in the cytoplasm connected with shrinkage of the cells. Gradual modifications in a cell's structure cause it to be discharged into the midgut lumen, where it disintegrates. Autophagy is involved in the disintegration of organelles. The transitions of apoptotic cells are described at the ultrastructural level. Immunostaining methods were used in order to indicate the early stages of apoptosis when DNA fragmentation, which results from apoptotic signaling cascades, occurs. © 2010 Zoological Society of Japan.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}