• dr hab. Piotr Łaszczyca
Stanowisko: Profesor Uczelni
Jednostka: Wydział Nauk Przyrodniczych
Adres: 40-007 Katowice, ul. Bankowa 9
Piętro: II
Numer pokoju: 218
Telefon: (32) 359 1728
E-mail: piotr.laszczyca@us.edu.pl
Spis publikacji: Spis wg CINiBA
Scopus Author ID: 6602531165
Publikacje z bazy Scopus
2024
Łaszczyca, P.; Nakonieczny, M.; Kędziorski, A.; Babczyńska, A.; Wiesner, M.
In: International Journal of Biometeorology, vol. 68, no. 6, pp. 1233-, 2024, ISSN: 00207128.
@article{2-s2.0-85187942660,
title = {Correction to: Towards understanding Cameraria ohridella (Lepidoptera: Gracillariidae) development: effects of microhabitat variability in naturally growing horse-chestnut tree canopy (International Journal of Biometeorology, (2021), 65, 10, (1647-1658), 10.1007/s00484-021-02119-8)},
author = { P. Łaszczyca and M. Nakonieczny and A. Kędziorski and A. Babczyńska and M. Wiesner},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85187942660&doi=10.1007%2fs00484-024-02656-y&partnerID=40&md5=7cc6e1a67533c0322243f7e545cd757d},
doi = {10.1007/s00484-024-02656-y},
issn = {00207128},
year = {2024},
date = {2024-01-01},
journal = {International Journal of Biometeorology},
volume = {68},
number = {6},
pages = {1233-},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {The article was published with an error in Eq. 3. Where the equation appears as: Logit linear transformation was based on the following formulas (Eqs. 3 and 4) (McDonald 2014): (Formula presented.) The equation should instead appear as: Logit linear transformation was based on the following formulas (Eqs. 3 and 4) (McDonald 2014): (Formula presented.) This correction stands to correct the original article. The original article has been corrected. © The Author(s) 2024.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The article was published with an error in Eq. 3. Where the equation appears as: Logit linear transformation was based on the following formulas (Eqs. 3 and 4) (McDonald 2014): (Formula presented.) The equation should instead appear as: Logit linear transformation was based on the following formulas (Eqs. 3 and 4) (McDonald 2014): (Formula presented.) This correction stands to correct the original article. The original article has been corrected. © The Author(s) 2024.
Kłosok, M.; de Carrillo, D. Gendosz; Łaszczyca, P.; Płociniczak, T.; Jedrzejowska-Szypulka, H.; Sawczyn, T.
Comparative Analysis of Fatty Acids Concentration in Liver and Muscle Tissues of Rats and Mice Journal Article
In: Applied Sciences (Switzerland), vol. 14, no. 8, 2024, ISSN: 20763417.
@article{2-s2.0-85192578545,
title = {Comparative Analysis of Fatty Acids Concentration in Liver and Muscle Tissues of Rats and Mice},
author = { M. Kłosok and D. Gendosz de Carrillo and P. Łaszczyca and T. Płociniczak and H. Jedrzejowska-Szypulka and T. Sawczyn},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85192578545&doi=10.3390%2fapp14083192&partnerID=40&md5=84a883599983a8ebcaf70bda39238b4b},
doi = {10.3390/app14083192},
issn = {20763417},
year = {2024},
date = {2024-01-01},
journal = {Applied Sciences (Switzerland)},
volume = {14},
number = {8},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {This study conducted a comparative analysis of fatty acids (FAs) concentration derived from polar (PL) and non-polar (NPL) lipids in the liver and muscle tissues of rats and mice. The objective was to elucidate species-specific differences in tissue FA distribution. Employing targeted GC/MS-based methodology, the study aimed to provide insights into conserved and species-specific aspects of lipid metabolism, thereby enhancing future experimental design, linked with comprehension of the interactions between diet, metabolism, and health. Results revealed markedly higher levels of total fatty acids (TFAs) derived from PL in mice skeletal muscle compared to rats and elevated saturated fatty acids (SFAs) levels in mice. Unsaturated fatty acid levels, mainly monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), were substantially higher in mice muscle across all lipid classes, resulting in a higher PUFA/TFA ratio in mice muscle. Detailed analysis of specific unsaturated fatty acids (C16:1; C18:1n9c; C18:2n6c; C22:6n3) indicated elevated levels in mice relative to rats. Conversely, rats exhibited higher SFA levels derived from the NPL fraction in the liver, particularly in myristic (C14:0), stearic (C18:0), and tricosanoic (C23:0) acids. Mice liver PL fractions displayed significantly elevated unsaturated FA levels, with notably higher MUFAs and lower PUFAs in NPL fractions compared to rats. Analysis of specific unsaturated FAs revealed higher levels of palmitoleic acid (C16:1) in mice, while rats exhibited increased linoleic (C18:2n6c) and linolenic (C18:3n3) acids. In conclusion, significant differences in FAs tissue distribution between rats and mice underscore the importance of considering species-specific FAs variations when utilizing these animal models and interpreting experimental results related to FA metabolism. © 2024 by the authors.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study conducted a comparative analysis of fatty acids (FAs) concentration derived from polar (PL) and non-polar (NPL) lipids in the liver and muscle tissues of rats and mice. The objective was to elucidate species-specific differences in tissue FA distribution. Employing targeted GC/MS-based methodology, the study aimed to provide insights into conserved and species-specific aspects of lipid metabolism, thereby enhancing future experimental design, linked with comprehension of the interactions between diet, metabolism, and health. Results revealed markedly higher levels of total fatty acids (TFAs) derived from PL in mice skeletal muscle compared to rats and elevated saturated fatty acids (SFAs) levels in mice. Unsaturated fatty acid levels, mainly monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), were substantially higher in mice muscle across all lipid classes, resulting in a higher PUFA/TFA ratio in mice muscle. Detailed analysis of specific unsaturated fatty acids (C16:1; C18:1n9c; C18:2n6c; C22:6n3) indicated elevated levels in mice relative to rats. Conversely, rats exhibited higher SFA levels derived from the NPL fraction in the liver, particularly in myristic (C14:0), stearic (C18:0), and tricosanoic (C23:0) acids. Mice liver PL fractions displayed significantly elevated unsaturated FA levels, with notably higher MUFAs and lower PUFAs in NPL fractions compared to rats. Analysis of specific unsaturated FAs revealed higher levels of palmitoleic acid (C16:1) in mice, while rats exhibited increased linoleic (C18:2n6c) and linolenic (C18:3n3) acids. In conclusion, significant differences in FAs tissue distribution between rats and mice underscore the importance of considering species-specific FAs variations when utilizing these animal models and interpreting experimental results related to FA metabolism. © 2024 by the authors.
2023
Łaszczyca, P.; Nakonieczny, M.; Kostecki, M.
In: Archives of Environmental Protection, vol. 49, no. 1, pp. 25-38, 2023, ISSN: 20834772, (2).
@article{2-s2.0-85152731846,
title = {Ecotoxicological biotests as tools for continuous monitoring of water quality in dam reservoir [Biotesty ekotoksykologiczne jako narzędzie do ciągłego monitoringu jakości wody w zbiornikach zaporowych]},
author = { P. Łaszczyca and M. Nakonieczny and M. Kostecki},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85152731846&doi=10.24425%2faep.2023.144734&partnerID=40&md5=06784cd914cf7e1dce946a219d9a27de},
doi = {10.24425/aep.2023.144734},
issn = {20834772},
year = {2023},
date = {2023-01-01},
journal = {Archives of Environmental Protection},
volume = {49},
number = {1},
pages = {25-38},
publisher = {Polska Akademia Nauk},
abstract = {Ecotoxicological biotests were applied in order to evaluate their suitability as early warning systems in the continuous monitoring of lowland shallow dam reservoirs located in Central Europe. The following biotests were used: Daphtoxkit F™magna, Algaltoxkit F™, Ostracodtoxkit F, Phytotoxkit and MARA Test. The experiment was conducted from July 2010 to December 2012 in Goczalkowice Reservoir (the Vistula River; Poland), serving as a model. For the analysis, 41 out of 52 measured water indices were used to assess its toxicity to living organisms. The results of biotests were correlated with 41 hydrochemical indices of water quality. The pattern of relationships among the result of biotest and hydrochemical indices as well as Factor Analysis (FA) and Primary Component Analysis (PCA) revealed that: i) signs of ecotoxicity detected with biotests were associated with either low flow periods or spring surface runoff of water; ii) single events of increased ecotoxicity in the depression areas behind saddle dam pump stations appeared after high flow periods; iii) elevated toxicity was accompanied by high concentrations of dissolved and suspended substances; iv) FA and PCA demonstrated correlations among the results of biotests and damming parameters, water conductivity, alkali and transitory metal metals (Ca, Fe, Cu, Zn), and several forms of nitrogen phosphorous and carbon compounds concentration. The relationships suggest that batteries of biotests may serve as a cost-effective tool for continuous monitoring of water quality in dam reservoirs and can detect effects of extreme hydrologic events, local toxic discharges, and signs of the trophic status of the reservoirs. © 2023. The Author(s).},
note = {2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Ecotoxicological biotests were applied in order to evaluate their suitability as early warning systems in the continuous monitoring of lowland shallow dam reservoirs located in Central Europe. The following biotests were used: Daphtoxkit F™magna, Algaltoxkit F™, Ostracodtoxkit F, Phytotoxkit and MARA Test. The experiment was conducted from July 2010 to December 2012 in Goczalkowice Reservoir (the Vistula River; Poland), serving as a model. For the analysis, 41 out of 52 measured water indices were used to assess its toxicity to living organisms. The results of biotests were correlated with 41 hydrochemical indices of water quality. The pattern of relationships among the result of biotest and hydrochemical indices as well as Factor Analysis (FA) and Primary Component Analysis (PCA) revealed that: i) signs of ecotoxicity detected with biotests were associated with either low flow periods or spring surface runoff of water; ii) single events of increased ecotoxicity in the depression areas behind saddle dam pump stations appeared after high flow periods; iii) elevated toxicity was accompanied by high concentrations of dissolved and suspended substances; iv) FA and PCA demonstrated correlations among the results of biotests and damming parameters, water conductivity, alkali and transitory metal metals (Ca, Fe, Cu, Zn), and several forms of nitrogen phosphorous and carbon compounds concentration. The relationships suggest that batteries of biotests may serve as a cost-effective tool for continuous monitoring of water quality in dam reservoirs and can detect effects of extreme hydrologic events, local toxic discharges, and signs of the trophic status of the reservoirs. © 2023. The Author(s).
2021
Poloczek, J.; Tarnawska, M.; Chełmecka, E.; Łaszczyca, P.; Gumprecht, J.; Stygar, D.
In: Nutrients, vol. 13, no. 10, 2021, ISSN: 20726643, (1).
@article{2-s2.0-85116966176,
title = {High fat, high sugar diet and djos bariatric surgery influence plasma levels of fetuin-b, growth differentiation factor-15, and pentraxin 3 in diet-induced obese sprague–dawley rats},
author = { J. Poloczek and M. Tarnawska and E. Chełmecka and P. Łaszczyca and J. Gumprecht and D. Stygar},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116966176&doi=10.3390%2fnu13103632&partnerID=40&md5=8f3785c8a936ad636f8864e971f4395f},
doi = {10.3390/nu13103632},
issn = {20726643},
year = {2021},
date = {2021-01-01},
journal = {Nutrients},
volume = {13},
number = {10},
publisher = {MDPI},
abstract = {The liver plays a central role in glucose and fatty acid metabolism and acts as an endocrine organ that secretes hepatokines with diverse systemic effects. The study aimed to examine the influence of duodenojejunal omega switch (DJOS) bariatric surgery in combination with different diets on glucose administration parameters and hepatokines levels. After 8 weeks on high fat, high sugar diet (HFS) or control diets (CD), Sprague–Dawley rats underwent DJOS or SHAM (control) surgery. For the next 8 weeks after the surgery, half of DJOS and SHAM-operated animals were kept on the same diet as before, and half had a diet change. The oral glucose tolerance test (OGTT) was performed three times: 8 weeks before and 4 and 8 weeks after surgery. Fetuin-B, growth differentiation factor-15 (GDF-15), pentraxin 3 (PTX3) plasma levels were analyzed. DJOS surgery had a beneficial effect on oral glucose tolerance test (OGTT) results and the area under the curve (AUCOGTT). The OGTT results depended on the time elapsed after the surgery, the type of diet used, the surgery performed, and the interaction between these factors. DJOS bariatric surgery reduced fetuin-B and GDF15 plasma levels. Interaction between the type of surgery performed and diet used influenced the fetuin-B and PTX-3 plasma levels. A dietary regime is essential to achieve therapeutic and clinical goals after bariatric surgery. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The liver plays a central role in glucose and fatty acid metabolism and acts as an endocrine organ that secretes hepatokines with diverse systemic effects. The study aimed to examine the influence of duodenojejunal omega switch (DJOS) bariatric surgery in combination with different diets on glucose administration parameters and hepatokines levels. After 8 weeks on high fat, high sugar diet (HFS) or control diets (CD), Sprague–Dawley rats underwent DJOS or SHAM (control) surgery. For the next 8 weeks after the surgery, half of DJOS and SHAM-operated animals were kept on the same diet as before, and half had a diet change. The oral glucose tolerance test (OGTT) was performed three times: 8 weeks before and 4 and 8 weeks after surgery. Fetuin-B, growth differentiation factor-15 (GDF-15), pentraxin 3 (PTX3) plasma levels were analyzed. DJOS surgery had a beneficial effect on oral glucose tolerance test (OGTT) results and the area under the curve (AUCOGTT). The OGTT results depended on the time elapsed after the surgery, the type of diet used, the surgery performed, and the interaction between these factors. DJOS bariatric surgery reduced fetuin-B and GDF15 plasma levels. Interaction between the type of surgery performed and diet used influenced the fetuin-B and PTX-3 plasma levels. A dietary regime is essential to achieve therapeutic and clinical goals after bariatric surgery. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Łaszczyca, P.; Nakonieczny, M.; Kędziorski, A.; Babczyńska, A.; Wiesner, M.
In: International Journal of Biometeorology, vol. 65, no. 10, pp. 1647-1658, 2021, ISSN: 00207128.
@article{2-s2.0-85104957867,
title = {Towards understanding Cameraria ohridella (Lepidoptera: Gracillariidae) development: effects of microhabitat variability in naturally growing horse-chestnut tree canopy},
author = { P. Łaszczyca and M. Nakonieczny and A. Kędziorski and A. Babczyńska and M. Wiesner},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104957867&doi=10.1007%2fs00484-021-02119-8&partnerID=40&md5=2b01bb6369bde5c53f17613274c9d44c},
doi = {10.1007/s00484-021-02119-8},
issn = {00207128},
year = {2021},
date = {2021-01-01},
journal = {International Journal of Biometeorology},
volume = {65},
number = {10},
pages = {1647-1658},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Dwelling intensity of horse-chestnut miner (Cameraria ohridella) larvae in various leaves insolation and temperature was measured to determine whether this pest’s development follows a predictable pattern or depends more on local microenvironment conditions. Mines growing on leaves of mature host plants (Aesculus hippocastanum L.) in their natural conditions were photographed for two consecutive generations of the pest and in two separated vegetation periods. Apart from meteorological data obtained from the nearest station, the temperature of intact and mined parts of sun-exposed and shaded leaf blades was measured at various daytimes throughout the experiment. Obtained sets of digital data were analysed and combined to model mine area growth as a function of degree-days sum by adopting of Verhulst logistic equation. We showed the predictive potential of our model based on experimental data, and it may be useful in the scheduling of pest control measures in natural conditions. Our analyses also revealed that despite significant differences in microenvironment conditions depending on mines’ insolation, the horse-chestnut miner larvae could partially compensate for them and complete their development at similar endpoints expressed as the cumulative sum of degree-days. We conclude that computer-aided analysis of photographic documentation of leaf-miner larval growth followed by mathematical modelling offers a noninvasive, reliable, and inexpensive alternative for monitoring local leaf-miners populations. © 2021, The Author(s).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Dwelling intensity of horse-chestnut miner (Cameraria ohridella) larvae in various leaves insolation and temperature was measured to determine whether this pest’s development follows a predictable pattern or depends more on local microenvironment conditions. Mines growing on leaves of mature host plants (Aesculus hippocastanum L.) in their natural conditions were photographed for two consecutive generations of the pest and in two separated vegetation periods. Apart from meteorological data obtained from the nearest station, the temperature of intact and mined parts of sun-exposed and shaded leaf blades was measured at various daytimes throughout the experiment. Obtained sets of digital data were analysed and combined to model mine area growth as a function of degree-days sum by adopting of Verhulst logistic equation. We showed the predictive potential of our model based on experimental data, and it may be useful in the scheduling of pest control measures in natural conditions. Our analyses also revealed that despite significant differences in microenvironment conditions depending on mines’ insolation, the horse-chestnut miner larvae could partially compensate for them and complete their development at similar endpoints expressed as the cumulative sum of degree-days. We conclude that computer-aided analysis of photographic documentation of leaf-miner larval growth followed by mathematical modelling offers a noninvasive, reliable, and inexpensive alternative for monitoring local leaf-miners populations. © 2021, The Author(s).
Babczyńska, A.; Tarnawska, M.; Łaszczyca, P.; Migula, P.; Łozowski, B.; Woźnica, A.; Irnazarow, I.; Augustyniak, M.
Stress proteins concentration in caged Cyprinus carpio as a tool to monitor ecological stability in a model dam reservoir Journal Article
In: Archives of Environmental Protection, vol. 47, no. 1, pp. 101-111, 2021, ISSN: 20834772, (1).
@article{2-s2.0-85102487461,
title = {Stress proteins concentration in caged Cyprinus carpio as a tool to monitor ecological stability in a model dam reservoir},
author = { A. Babczyńska and M. Tarnawska and P. Łaszczyca and P. Migula and B. Łozowski and A. Woźnica and I. Irnazarow and M. Augustyniak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102487461&doi=10.24425%2faep.2021.136452&partnerID=40&md5=5aafdbafbb462de53a29be6faf4a67ef},
doi = {10.24425/aep.2021.136452},
issn = {20834772},
year = {2021},
date = {2021-01-01},
journal = {Archives of Environmental Protection},
volume = {47},
number = {1},
pages = {101-111},
publisher = {Polska Akademia Nauk},
abstract = {Variability of stress proteins concentration in caged carp exposed to transplantation experiment model dam reservoir was caused only by natural (climatic and biological) conditions. Thus, the reference data of stress proteins concentration range in young carp individuals were obtained. Metallothionein, HSP70 and HSP90 protein concentrations as biomarkers were assayed in the livers, gills and muscles of six-month-old (summer) or nine-month-old (autumn) carp individuals in relation to the site of encaging, season (summer or autumn), the term of sampling (1; 2 or 3 weeks after the transplantation) and tissue. Physicochemical analyses of the condition of water as well as pollution detection were conducted during each stage of the experiment. As the result of this study, the range of the variability of the stress protein concentration in young carp individuals was obtained. According to the analyses of the aquatic conditions of a reservoir with no detectable pollutants, we conclude that the variability in the stress protein concentration levels in the groups that were compared is solely the result of the natural conditions. Future regular monitoring of the reservoir using the transplantation method and young carp individuals will be both possible and reliable. Moreover, the range of variability in the stress protein concentrations that were measured in the young C. carpio individuals acquired from the model dam reservoir in relation to all of the studied factors may be applied in the monitoring of any other similar reservoir. © 2021. The Author(s).},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Variability of stress proteins concentration in caged carp exposed to transplantation experiment model dam reservoir was caused only by natural (climatic and biological) conditions. Thus, the reference data of stress proteins concentration range in young carp individuals were obtained. Metallothionein, HSP70 and HSP90 protein concentrations as biomarkers were assayed in the livers, gills and muscles of six-month-old (summer) or nine-month-old (autumn) carp individuals in relation to the site of encaging, season (summer or autumn), the term of sampling (1; 2 or 3 weeks after the transplantation) and tissue. Physicochemical analyses of the condition of water as well as pollution detection were conducted during each stage of the experiment. As the result of this study, the range of the variability of the stress protein concentration in young carp individuals was obtained. According to the analyses of the aquatic conditions of a reservoir with no detectable pollutants, we conclude that the variability in the stress protein concentration levels in the groups that were compared is solely the result of the natural conditions. Future regular monitoring of the reservoir using the transplantation method and young carp individuals will be both possible and reliable. Moreover, the range of variability in the stress protein concentrations that were measured in the young C. carpio individuals acquired from the model dam reservoir in relation to all of the studied factors may be applied in the monitoring of any other similar reservoir. © 2021. The Author(s).
2019
Tarnawska, M.; Augustyniak, M.; Łaszczyca, P.; Migula, P.; Irnazarow, I.; Krzyżowski, M.; Babczyńska, A.
Immune response of juvenile common carp (Cyprinus carpio L.) exposed to a mixture of sewage chemicals Journal Article
In: Fish and Shellfish Immunology, vol. 88, pp. 17-27, 2019, ISSN: 10504648, (6).
@article{2-s2.0-85062391000,
title = {Immune response of juvenile common carp (Cyprinus carpio L.) exposed to a mixture of sewage chemicals},
author = { M. Tarnawska and M. Augustyniak and P. Łaszczyca and P. Migula and I. Irnazarow and M. Krzyżowski and A. Babczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062391000&doi=10.1016%2fj.fsi.2019.02.049&partnerID=40&md5=d42712874f989fa17f5c77ff077e7bc5},
doi = {10.1016/j.fsi.2019.02.049},
issn = {10504648},
year = {2019},
date = {2019-01-01},
journal = {Fish and Shellfish Immunology},
volume = {88},
pages = {17-27},
publisher = {Academic Press},
abstract = {Pharmaceuticals and household chemicals are important components of municipal sewage. Many of them are biologically active, disrupting not only hormonal regulation of aquatic animals but also, indirectly, disturbing their immunological protection. In the environment, chemicals rarely act as individual substances, but as elements of mixtures. Therefore, the aim of this study was to check whether the acute laboratory exposure of common carp juveniles to a mixture of ibuprofen, sodium dodecyl sulphate (SDS), dimethyl sulfoxide (DMSO) and 17 α-ethynylestradiol in increasing concentrations, modifies the levels of innate immunity (lysozyme; C-reactive protein) as well as general stress (metallothioneins; heat shock proteins HSP70) markers in brain, liver, gills, spleen and mucus. The levels of the markers were measured by an immunodetection technique. Not only do the pharmaceuticals and household chemicals impair immunological reactions of young carp in various tissues but also do that in a concentration-dependent manner in the liver, gills, spleen and mucus. This has a very important implication, since it may result in higher sensitivity of young fish to pathogens due to energy allocation to defence processes. The comparisons of the pattern of stress reactions in the studied organ samples indicated that mucus appeared to be a good, non-invasive material for monitoring of environmental state and fish conditions. © 2019},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Pharmaceuticals and household chemicals are important components of municipal sewage. Many of them are biologically active, disrupting not only hormonal regulation of aquatic animals but also, indirectly, disturbing their immunological protection. In the environment, chemicals rarely act as individual substances, but as elements of mixtures. Therefore, the aim of this study was to check whether the acute laboratory exposure of common carp juveniles to a mixture of ibuprofen, sodium dodecyl sulphate (SDS), dimethyl sulfoxide (DMSO) and 17 α-ethynylestradiol in increasing concentrations, modifies the levels of innate immunity (lysozyme; C-reactive protein) as well as general stress (metallothioneins; heat shock proteins HSP70) markers in brain, liver, gills, spleen and mucus. The levels of the markers were measured by an immunodetection technique. Not only do the pharmaceuticals and household chemicals impair immunological reactions of young carp in various tissues but also do that in a concentration-dependent manner in the liver, gills, spleen and mucus. This has a very important implication, since it may result in higher sensitivity of young fish to pathogens due to energy allocation to defence processes. The comparisons of the pattern of stress reactions in the studied organ samples indicated that mucus appeared to be a good, non-invasive material for monitoring of environmental state and fish conditions. © 2019
2015
Augustyniak, M.; Nocon, T.; Kędziorski, A.; Łaszczyca, P.; Sawczyn, T.; Tarnawska, M.; Zawisza-Raszka, A.
DNA damage in grasshopper Chorthippus brunneus (Orthoptera) hatchlings following paraquat exposure Journal Article
In: Chemosphere, vol. 125, pp. 212-219, 2015, ISSN: 00456535, (15).
@article{2-s2.0-84923569075,
title = {DNA damage in grasshopper Chorthippus brunneus (Orthoptera) hatchlings following paraquat exposure},
author = { M. Augustyniak and T. Nocon and A. Kędziorski and P. Łaszczyca and T. Sawczyn and M. Tarnawska and A. Zawisza-Raszka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923569075&doi=10.1016%2fj.chemosphere.2014.12.069&partnerID=40&md5=f1d2caec2debb3c806d9441a50130558},
doi = {10.1016/j.chemosphere.2014.12.069},
issn = {00456535},
year = {2015},
date = {2015-01-01},
journal = {Chemosphere},
volume = {125},
pages = {212-219},
publisher = {Elsevier Ltd},
abstract = {Comet assay was applied to study genotoxic damage induced by paraquat (PQ) in brain cells of Chorthippus brunneus (Insecta: Orthoptera) hatchlings. Percentage of the comet fluorescence in the tail (TDNA), length of the comet tail (TL) and Olive tail moment (OTM) were used for quantitative assessment of the DNA damage. Multiple regression analysis supplemented standard statistical elaboration of the results. Increasing PQ concentrations applied either directly to the brain cells suspension (10; 50; and 250. μM PQ final concentration - in vitro protocol) or indirectly (50; 250; and 1250. μM PQ final concentration - in vivo protocol) provoked significant increase of oxidative damage to DNA (higher median TDNA and OTM values). The damage increased with time of exposure (0; 5; 15; and 30. min) following in vitro application, but decreased in longer interval (3 vs 24. h) after in vivo administration of paraquat. On contrary, median TL values did not correlate with paraquat concentration irrespectively of the exposure protocol. Possible reason of this discrepancy in light of paraquat toxicity is discussed. © 2015 Elsevier Ltd.},
note = {15},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Comet assay was applied to study genotoxic damage induced by paraquat (PQ) in brain cells of Chorthippus brunneus (Insecta: Orthoptera) hatchlings. Percentage of the comet fluorescence in the tail (TDNA), length of the comet tail (TL) and Olive tail moment (OTM) were used for quantitative assessment of the DNA damage. Multiple regression analysis supplemented standard statistical elaboration of the results. Increasing PQ concentrations applied either directly to the brain cells suspension (10; 50; and 250. μM PQ final concentration - in vitro protocol) or indirectly (50; 250; and 1250. μM PQ final concentration - in vivo protocol) provoked significant increase of oxidative damage to DNA (higher median TDNA and OTM values). The damage increased with time of exposure (0; 5; 15; and 30. min) following in vitro application, but decreased in longer interval (3 vs 24. h) after in vivo administration of paraquat. On contrary, median TL values did not correlate with paraquat concentration irrespectively of the exposure protocol. Possible reason of this discrepancy in light of paraquat toxicity is discussed. © 2015 Elsevier Ltd.
2014
Łozowski, B.; Kędziorski, A.; Nakonieczny, M.; Łaszczyca, P.
Parnassius apollo last-instar larvae development prediction by analysis of weather condition as a tool in the species' conservation Journal Article
In: Comptes Rendus - Biologies, vol. 337, no. 5, pp. 325-331, 2014, ISSN: 16310691, (8).
@article{2-s2.0-84901241774,
title = {Parnassius apollo last-instar larvae development prediction by analysis of weather condition as a tool in the species' conservation},
author = { B. Łozowski and A. Kędziorski and M. Nakonieczny and P. Łaszczyca},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901241774&doi=10.1016%2fj.crvi.2014.03.004&partnerID=40&md5=c15d9fa248f59d5b88e594a7a6d4ae43},
doi = {10.1016/j.crvi.2014.03.004},
issn = {16310691},
year = {2014},
date = {2014-01-01},
journal = {Comptes Rendus - Biologies},
volume = {337},
number = {5},
pages = {325-331},
publisher = {Elsevier Masson SAS},
abstract = {Parnassius apollo (Lepidoptera: Papilionidae) has already disappeared or is under threat of extinction in many of its former habitats. It has been documented that weather conditions - anomalies in particular - contributed to this process. In this study, we combined developmental data obtained previously for the last-instar Apollo larvae (collected in 1996; 1997; and 2003) with corresponding meteorological data to assess the effects of ambient temperature and rainfall episodes on the duration and the completion of the instar. For comparing the temperature effect, we applied the degree-day concept. We found significant positive correlation between the number of rainy days during the instar development (x) and its duration time (y): y = 8.293 + 0.936x (± 2.813) (r = 0.662; P < 10-7). Logarithmic transformation of the growth curves of the last-instar Apollo larvae revealed that there was no difference in growth among females; however, there was slower growth of males in 2003 in comparison to 1996. Growth (y) of female Apollo larvae as a function of instar duration (x) can by described by one common equation, irrespectively of the year: y = 317.6 + 502.3 lnx (± 263.3) (r = 0.82; P < 10 -4). © 2014 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.},
note = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Parnassius apollo (Lepidoptera: Papilionidae) has already disappeared or is under threat of extinction in many of its former habitats. It has been documented that weather conditions - anomalies in particular - contributed to this process. In this study, we combined developmental data obtained previously for the last-instar Apollo larvae (collected in 1996; 1997; and 2003) with corresponding meteorological data to assess the effects of ambient temperature and rainfall episodes on the duration and the completion of the instar. For comparing the temperature effect, we applied the degree-day concept. We found significant positive correlation between the number of rainy days during the instar development (x) and its duration time (y): y = 8.293 + 0.936x (± 2.813) (r = 0.662; P < 10-7). Logarithmic transformation of the growth curves of the last-instar Apollo larvae revealed that there was no difference in growth among females; however, there was slower growth of males in 2003 in comparison to 1996. Growth (y) of female Apollo larvae as a function of instar duration (x) can by described by one common equation, irrespectively of the year: y = 317.6 + 502.3 lnx (± 263.3) (r = 0.82; P < 10 -4). © 2014 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.
2005
Augustyniak, M.; Babczyńska, A.; Migula, P.; Wilczek, G.; Łaszczyca, P.; Kafel, A.; Augustyniak, M. G.
In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, vol. 141, no. 4, pp. 412-419, 2005, ISSN: 15320456, (33).
@article{2-s2.0-27744505338,
title = {Joint effects of dimethoate and heavy metals on metabolic responses in a grasshopper (Chorthippus brunneus) from a heavy metals pollution gradient},
author = { M. Augustyniak and A. Babczyńska and P. Migula and G. Wilczek and P. Łaszczyca and A. Kafel and M.G. Augustyniak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-27744505338&doi=10.1016%2fj.cbpc.2005.09.007&partnerID=40&md5=1761544f1ffb0849417b3d03dcae3174},
doi = {10.1016/j.cbpc.2005.09.007},
issn = {15320456},
year = {2005},
date = {2005-01-01},
journal = {Comparative Biochemistry and Physiology - C Toxicology and Pharmacology},
volume = {141},
number = {4},
pages = {412-419},
publisher = {Elsevier Inc.},
abstract = {We studied how an exposure to an additional stressing factor-dimethoate, might affect detoxifying ability of grasshoppers collected at 5 meadow sites located along a heavy metal pollution gradient. Activities of esterases and enzymes linked with glutathione (GSH) metabolism were assayed 24 h after topical treatment with 0.32 μg dimethoate per insect. Inhibition of acetylcholinesterase (AChE) reaches nearly 50% of the value stated in untreated insects, without significant site-dependent differences. The pesticide also caused a significant decrease in activities of glutathione peroxidase (GPx) followed by a decrease in GSH levels in grasshoppers from all assayed groups, demonstrating high sensitivity of glutathione-dependent metabolism to the additional stressing factor. In the case of glutathione reductase (GR) and carboxylesterases (CarE) the fall of activity was shown especially in insects from less polluted meadows and the reference site. Glutathione reductase (GR) activity in individuals treated with dimethoate did not decrease only in insects from the most contaminated site I. This might suggest the trade-off mechanisms adapting grasshoppers to life in seriously polluted environments. © 2005 Elsevier Inc. All rights reserved.},
note = {33},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We studied how an exposure to an additional stressing factor-dimethoate, might affect detoxifying ability of grasshoppers collected at 5 meadow sites located along a heavy metal pollution gradient. Activities of esterases and enzymes linked with glutathione (GSH) metabolism were assayed 24 h after topical treatment with 0.32 μg dimethoate per insect. Inhibition of acetylcholinesterase (AChE) reaches nearly 50% of the value stated in untreated insects, without significant site-dependent differences. The pesticide also caused a significant decrease in activities of glutathione peroxidase (GPx) followed by a decrease in GSH levels in grasshoppers from all assayed groups, demonstrating high sensitivity of glutathione-dependent metabolism to the additional stressing factor. In the case of glutathione reductase (GR) and carboxylesterases (CarE) the fall of activity was shown especially in insects from less polluted meadows and the reference site. Glutathione reductase (GR) activity in individuals treated with dimethoate did not decrease only in insects from the most contaminated site I. This might suggest the trade-off mechanisms adapting grasshoppers to life in seriously polluted environments. © 2005 Elsevier Inc. All rights reserved.
2004
Migula, P.; Łaszczyca, P.; Augustyniak, M.; Wilczek, G.; Rozpędek, K.; Kafel, A.; Wołoszyn, M.
Antioxidative defence enzymes in beetles from a metal pollution gradient Journal Article
In: Biologia - Section Zoology, vol. 59, no. 5, pp. 645-654, 2004, ISSN: 13356380, (51).
@article{2-s2.0-10044240191,
title = {Antioxidative defence enzymes in beetles from a metal pollution gradient},
author = { P. Migula and P. Łaszczyca and M. Augustyniak and G. Wilczek and K. Rozpędek and A. Kafel and M. Wołoszyn},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-10044240191&partnerID=40&md5=a309d39f85323f5fcc87cca33f05e9d3},
issn = {13356380},
year = {2004},
date = {2004-01-01},
journal = {Biologia - Section Zoology},
volume = {59},
number = {5},
pages = {645-654},
abstract = {Oxidative stress in insects may result from an imbalance of oxidants and antioxidants under a significant impact of metals. We studied variation in antioxidant enzyme activity in four species of beetles representing carnivores, carrion eaters, omnivores and phytophages in response to heavy metal pollution. Insects were collected at five forest sites along a gradient of heavy metal pollution in southern Poland. Assayed activity of superoxide dismutase (SOD), glutathione reductase (GR), glutathione S-transferase (GST), Se-dependent (GPOX) and Se-independent (GSTP) glutathione peroxidases and catalase (CAT) correlated with metal pollution levels and body concentrations of metals (Pb; Zn; Cu; Cd). Antioxidative enzyme activity patterns were species-dependent and were correlated with the levels of metal pollution or the body loads of metals. Correlations were predominantly positive in the case of Pb and Cd, and both positive and negative in the case of Zn. The largest difference between species was in the GST activity of the carnivorous Pterostichus oblongopunctatus and the phytophagous Phyllobius betulae. Activity of GSH-dependent peroxidases and GR was low in most of the species studied. Beetles from metal-contaminated sites showed higher within-species variance of enzyme activity, probably due to their higher polymorphism of antioxidative enzymes.},
note = {51},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Oxidative stress in insects may result from an imbalance of oxidants and antioxidants under a significant impact of metals. We studied variation in antioxidant enzyme activity in four species of beetles representing carnivores, carrion eaters, omnivores and phytophages in response to heavy metal pollution. Insects were collected at five forest sites along a gradient of heavy metal pollution in southern Poland. Assayed activity of superoxide dismutase (SOD), glutathione reductase (GR), glutathione S-transferase (GST), Se-dependent (GPOX) and Se-independent (GSTP) glutathione peroxidases and catalase (CAT) correlated with metal pollution levels and body concentrations of metals (Pb; Zn; Cu; Cd). Antioxidative enzyme activity patterns were species-dependent and were correlated with the levels of metal pollution or the body loads of metals. Correlations were predominantly positive in the case of Pb and Cd, and both positive and negative in the case of Zn. The largest difference between species was in the GST activity of the carnivorous Pterostichus oblongopunctatus and the phytophagous Phyllobius betulae. Activity of GSH-dependent peroxidases and GR was low in most of the species studied. Beetles from metal-contaminated sites showed higher within-species variance of enzyme activity, probably due to their higher polymorphism of antioxidative enzymes.
Łaszczyca, P.; Augustyniak, M.; Babczyńska, A.; Bednarska, K.; Kafel, A.; Migula, P.; Wilczek, G.; Witas, I.
Profiles of enzymatic activity in earthworms from zinc, lead and cadmium polluted areas near Olkusz (Poland) Journal Article
In: Environment International, vol. 30, no. 7, pp. 901-910, 2004, ISSN: 01604120, (113).
@article{2-s2.0-2942670066,
title = {Profiles of enzymatic activity in earthworms from zinc, lead and cadmium polluted areas near Olkusz (Poland)},
author = { P. Łaszczyca and M. Augustyniak and A. Babczyńska and K. Bednarska and A. Kafel and P. Migula and G. Wilczek and I. Witas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2942670066&doi=10.1016%2fj.envint.2004.02.006&partnerID=40&md5=fb1bf084ee1cffc308a2059a62a4a95c},
doi = {10.1016/j.envint.2004.02.006},
issn = {01604120},
year = {2004},
date = {2004-01-01},
journal = {Environment International},
volume = {30},
number = {7},
pages = {901-910},
publisher = {Elsevier Ltd},
abstract = {The aim of the study was to determine whether there are signs of adaptation of soil fauna to a gradient of heavy metal contamination. Earthworms Aporrectodea caliginosa, Lumbricus terrestris and Eisenia fetida were collected during the spring and summer of 2000 and 2001 from meadow sites situated between 2 and 32 km from the Bukowno-Olkusz complex of zinc-lead ore mines and smelters. The heavy metal content in the soil near smelters reaches 10,500 mg/kg (d.w.) for Zn, 2600 mg/kg for Pb and 81.9 mg/kg for Cd. The sites differ with respect to species composition of earthworm community, with A. caliginosa being dominant. Complete data was obtained only for A. caliginosa, since other species were not abundant at all investigated sites during the whole period of investigation. The body burdens of Zn, Pb, Cd and Cu in A. caliginosa reached 1500, 100, 220 and 10 μg/g, respectively, in the vicinity of the smelter (2-4 km), and decreased to 400, 2, 36 and 6 μg/g at the most distant site (32 km). Cadmium and lead content was significantly elevated in the whole body of L. terrestris collected at the site 2.5 km distant from the smelters when compared to more distant sites, while in E. fetida only the body burden of cadmium was elevated at the nearest site compared to the next site of transect. Activities of glutathione peroxidase (GPX; EC 1.11.1.9) against hydrogen peroxide (H2O2) or cumene hydroperoxide (cumOOH), glutathione reductase (GR; EC 1.6.4.2), glutathione S-transferase (GST; EC 2.5.1.18) and catalase (CAT; EC 1.11.1.6) were assayed in postmitochondrial supernatant obtained from whole body homogenates. Seasonal and annual variations of enzyme activity were reflected by higher GPX activity in the late summer of 2001 in comparison with the spring and summer of 2000. This may reflect severe drought in the spring and summer of 2000. The activity of both GPX isozymes, GR and GST in A. caliginosa and L. terrestris increased with increasing distance from the smelters and reached maximum at sites III and IV (4 and 8 km from the smelters; respectively) and then it decreased in the animals from site V (32 km). These may be the effects of antagonism between the enzyme inducing and enzyme inhibiting action of smelter emissions, a phenomenon known as a hormetic effect. It is postulated here that this effect is of diagnostic value for metal pollution biomonitoring. © 2004 Elsevier Ltd. All rights reserved.},
note = {113},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of the study was to determine whether there are signs of adaptation of soil fauna to a gradient of heavy metal contamination. Earthworms Aporrectodea caliginosa, Lumbricus terrestris and Eisenia fetida were collected during the spring and summer of 2000 and 2001 from meadow sites situated between 2 and 32 km from the Bukowno-Olkusz complex of zinc-lead ore mines and smelters. The heavy metal content in the soil near smelters reaches 10,500 mg/kg (d.w.) for Zn, 2600 mg/kg for Pb and 81.9 mg/kg for Cd. The sites differ with respect to species composition of earthworm community, with A. caliginosa being dominant. Complete data was obtained only for A. caliginosa, since other species were not abundant at all investigated sites during the whole period of investigation. The body burdens of Zn, Pb, Cd and Cu in A. caliginosa reached 1500, 100, 220 and 10 μg/g, respectively, in the vicinity of the smelter (2-4 km), and decreased to 400, 2, 36 and 6 μg/g at the most distant site (32 km). Cadmium and lead content was significantly elevated in the whole body of L. terrestris collected at the site 2.5 km distant from the smelters when compared to more distant sites, while in E. fetida only the body burden of cadmium was elevated at the nearest site compared to the next site of transect. Activities of glutathione peroxidase (GPX; EC 1.11.1.9) against hydrogen peroxide (H2O2) or cumene hydroperoxide (cumOOH), glutathione reductase (GR; EC 1.6.4.2), glutathione S-transferase (GST; EC 2.5.1.18) and catalase (CAT; EC 1.11.1.6) were assayed in postmitochondrial supernatant obtained from whole body homogenates. Seasonal and annual variations of enzyme activity were reflected by higher GPX activity in the late summer of 2001 in comparison with the spring and summer of 2000. This may reflect severe drought in the spring and summer of 2000. The activity of both GPX isozymes, GR and GST in A. caliginosa and L. terrestris increased with increasing distance from the smelters and reached maximum at sites III and IV (4 and 8 km from the smelters; respectively) and then it decreased in the animals from site V (32 km). These may be the effects of antagonism between the enzyme inducing and enzyme inhibiting action of smelter emissions, a phenomenon known as a hormetic effect. It is postulated here that this effect is of diagnostic value for metal pollution biomonitoring. © 2004 Elsevier Ltd. All rights reserved.
1996
Łaszczyca, P.; Kawka-Serwecińska, E.; Witas, I.; Doleżych, B.; Falkus, B.; Mekail, A.; Ziółkowska, B.; Madej, P.; Migula, P.
Lipid peroxidation and activity of antioxidative enzymes in the rat model of ozone therapy. Journal Article
In: Materia medica Polona. Polish journal of medicine and pharmacy, vol. 28, no. 4, pp. 155-160, 1996, ISSN: 00255246, (8).
@article{2-s2.0-0030252327,
title = {Lipid peroxidation and activity of antioxidative enzymes in the rat model of ozone therapy.},
author = { P. Łaszczyca and E. Kawka-Serwecińska and I. Witas and B. Doleżych and B. Falkus and A. Mekail and B. Ziółkowska and P. Madej and P. Migula},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030252327&partnerID=40&md5=f6a9c197867425e982bec688275688d5},
issn = {00255246},
year = {1996},
date = {1996-01-01},
journal = {Materia medica Polona. Polish journal of medicine and pharmacy},
volume = {28},
number = {4},
pages = {155-160},
abstract = {Hypothetical, therapeutic effects of ozone were investigated in an animal model. One ml of oxygen or mixture of 40 micrograms ozone with oxygen were injected intraperitoneally to male rats for 10 days. Previously, rats had been poisoned with 50 ppm Cd2+ in drinking water for 12 weeks. Exhaustive treadmill running was applied to some animals before sacrification. Ozone injections increased iron-ascorbate-stimulated lipid peroxidation (LPO) in the liver and kidney, catalase (CAT) activity in the heart and glutathione S-transferase (GST) activity in the heart, kidney and liver. Oxygen increased GST activity in the brain and reduced glutathione peroxidase (GPX) activity in the kidney. Cadmium enhanced LPO in the liver and GST activity in the brain, heart, kidney and liver. In contrast to ozone, cadmium inhibited GPX activity in the brain, kidney and liver. Cadmium combined with ozone enhanced the changes of GPX activity in the kidney and liver, that of GST activity in the heart, kidney and liver as well as of CAT activity and LPO in kidney. The results suggest that ozone injections combined with tested factors may provoke an oxidative stress. The effects of ozone therapy can not be explained as the results of ozone action on the antioxidative enzymes in rat.},
note = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Hypothetical, therapeutic effects of ozone were investigated in an animal model. One ml of oxygen or mixture of 40 micrograms ozone with oxygen were injected intraperitoneally to male rats for 10 days. Previously, rats had been poisoned with 50 ppm Cd2+ in drinking water for 12 weeks. Exhaustive treadmill running was applied to some animals before sacrification. Ozone injections increased iron-ascorbate-stimulated lipid peroxidation (LPO) in the liver and kidney, catalase (CAT) activity in the heart and glutathione S-transferase (GST) activity in the heart, kidney and liver. Oxygen increased GST activity in the brain and reduced glutathione peroxidase (GPX) activity in the kidney. Cadmium enhanced LPO in the liver and GST activity in the brain, heart, kidney and liver. In contrast to ozone, cadmium inhibited GPX activity in the brain, kidney and liver. Cadmium combined with ozone enhanced the changes of GPX activity in the kidney and liver, that of GST activity in the heart, kidney and liver as well as of CAT activity and LPO in kidney. The results suggest that ozone injections combined with tested factors may provoke an oxidative stress. The effects of ozone therapy can not be explained as the results of ozone action on the antioxidative enzymes in rat.
1995
Łaszczyca, P.; Kawka-Serwecińska, E.; Witas, I.; Doleżych, B.; Migula, P.
Iron ascorbate-stimulated lipid peroxidation in vitro. Why is the method controversial? Journal Article
In: General Physiology and Biophysics, vol. 14, no. 1, pp. 3-18, 1995, ISSN: 02315882, (5).
@article{2-s2.0-0029014509,
title = {Iron ascorbate-stimulated lipid peroxidation in vitro. Why is the method controversial?},
author = { P. Łaszczyca and E. Kawka-Serwecińska and I. Witas and B. Doleżych and P. Migula},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029014509&partnerID=40&md5=cae792886345504ee1224eda707729d2},
issn = {02315882},
year = {1995},
date = {1995-01-01},
journal = {General Physiology and Biophysics},
volume = {14},
number = {1},
pages = {3-18},
abstract = {In vitro generation of thiobarbituric acid reactive substances (TBARS) is frequently used to assess organ susceptibility to lipid peroxidation. The yield of TBARS is severalfold enhanced by an addition of iron ions with reductors or chelators such as ascorbate, NADPH, ADP or pyrophosphate. The process cannot be interpreted in a simple way, since it involves several enzymatic and nonenzymatic reactions. There are no clear interpretations of the ambiguous effects of denaturating factors and chelating agents on TBARS generation. Also controversy arises from the cirvilinear relationship between the homogenate concentration and the yield of TBARS. This has been modelled in the present work by combining two functions describing the sequential reaction with two limiting steps. One of them is related to catalytic action of iron and ascorbate, while the other to an enzyme, possibly phospholipase A2, as has been suggested by some investigators. Two models should be considered since it is impossible to decide which kinetic equation should predominate in the model. Nevertheless, the model reflects kinetic properties of the process. The effects of catalyst concentration and some other modification upon the yield of TBARS were also investigated experimentally. The results of experiments and modelling showed that the analytical procedures used by investigators need standardisation as the results obtained under a variety of procedures may reflect quite different properties of the living systems.},
note = {5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In vitro generation of thiobarbituric acid reactive substances (TBARS) is frequently used to assess organ susceptibility to lipid peroxidation. The yield of TBARS is severalfold enhanced by an addition of iron ions with reductors or chelators such as ascorbate, NADPH, ADP or pyrophosphate. The process cannot be interpreted in a simple way, since it involves several enzymatic and nonenzymatic reactions. There are no clear interpretations of the ambiguous effects of denaturating factors and chelating agents on TBARS generation. Also controversy arises from the cirvilinear relationship between the homogenate concentration and the yield of TBARS. This has been modelled in the present work by combining two functions describing the sequential reaction with two limiting steps. One of them is related to catalytic action of iron and ascorbate, while the other to an enzyme, possibly phospholipase A2, as has been suggested by some investigators. Two models should be considered since it is impossible to decide which kinetic equation should predominate in the model. Nevertheless, the model reflects kinetic properties of the process. The effects of catalyst concentration and some other modification upon the yield of TBARS were also investigated experimentally. The results of experiments and modelling showed that the analytical procedures used by investigators need standardisation as the results obtained under a variety of procedures may reflect quite different properties of the living systems.
1989
Łaszczyca, P.
The activity of mitochondrial enzymes in the muscles of rats subjected to physical training and subchronical intoxication with lead and zinc Journal Article
In: Acta Physiologica Polonica, vol. 40, no. 5-6, pp. 544-551, 1989, ISSN: 00446033, (6).
@article{2-s2.0-0024941482,
title = {The activity of mitochondrial enzymes in the muscles of rats subjected to physical training and subchronical intoxication with lead and zinc},
author = { P. Łaszczyca},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024941482&partnerID=40&md5=b0b52f2447bb0d915f2f3e6c76cd5f25},
issn = {00446033},
year = {1989},
date = {1989-01-01},
journal = {Acta Physiologica Polonica},
volume = {40},
number = {5-6},
pages = {544-551},
abstract = {[No abstract available]},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
[No abstract available]
Łaszczyca, P.; Lehouelleur, J.; Bruner, J.
Low calcium treatment provokes persistent spiking in tonic crayfish muscle. Journal Article
In: General physiology and biophysics, vol. 8, no. 2, pp. 171-176, 1989, ISSN: 02315882.
@article{2-s2.0-0024643710,
title = {Low calcium treatment provokes persistent spiking in tonic crayfish muscle.},
author = { P. Łaszczyca and J. Lehouelleur and J. Bruner},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024643710&partnerID=40&md5=87cce4bdd30f2ff7804f9d83bbd4baad},
issn = {02315882},
year = {1989},
date = {1989-01-01},
journal = {General physiology and biophysics},
volume = {8},
number = {2},
pages = {171-176},
abstract = {[No abstract available]},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
[No abstract available]
1988
Lehouelleur, J.; Łaszczyca, P.; Bruner, J.
Sodium-dependent depolarizing potentials in veratrinized crayfish muscle fibres Journal Article
In: Neuroscience Letters, vol. 89, no. 3, pp. 299-304, 1988, ISSN: 03043940, (1).
@article{2-s2.0-0023948644,
title = {Sodium-dependent depolarizing potentials in veratrinized crayfish muscle fibres},
author = { J. Lehouelleur and P. Łaszczyca and J. Bruner},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023948644&doi=10.1016%2f0304-3940%2888%2990543-5&partnerID=40&md5=6d447d0fcd4a419dab7acc5387f7de2c},
doi = {10.1016/0304-3940(88)90543-5},
issn = {03043940},
year = {1988},
date = {1988-01-01},
journal = {Neuroscience Letters},
volume = {89},
number = {3},
pages = {299-304},
abstract = {In the slow abdominal flexor muscle fibers of the crayfish Procambarus clarkii, veratrine or veratridine applied during several minutes produced the persistent transformation of the muscle fibre from a non-spiking into a Ca-dependent spiking one and spikes were followed by a long-duration depolarization. The long-duration potential depends on external Na+ but is not blocked by 30 μM tetrodotoxin (TTX). In solutions containing normal concentrations of Na+ (207 mM) the absence of Ca2+ or the presence of calcium channel blockers abolished both potentials. The results show that alkaloid toxins reveal a Ca2+-dependent, TTX-resistant Na+ conductance in crayfish tonic muscle fibres. © 1988.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In the slow abdominal flexor muscle fibers of the crayfish Procambarus clarkii, veratrine or veratridine applied during several minutes produced the persistent transformation of the muscle fibre from a non-spiking into a Ca-dependent spiking one and spikes were followed by a long-duration depolarization. The long-duration potential depends on external Na+ but is not blocked by 30 μM tetrodotoxin (TTX). In solutions containing normal concentrations of Na+ (207 mM) the absence of Ca2+ or the presence of calcium channel blockers abolished both potentials. The results show that alkaloid toxins reveal a Ca2+-dependent, TTX-resistant Na+ conductance in crayfish tonic muscle fibres. © 1988.
1984
Sawicka, T. J.; Łaszczyca, P.; Smylla, B.; Jethon, Z.
Metabolic adaptation of muscles to exercise, vibration and raised temperature under the influence of cernitins Journal Article
In: Acta Physiologica Polonica, vol. 35, no. 2, pp. 141-150, 1984, ISSN: 00446033.
@article{2-s2.0-0021628753,
title = {Metabolic adaptation of muscles to exercise, vibration and raised temperature under the influence of cernitins},
author = { T.J. Sawicka and P. Łaszczyca and B. Smylla and Z. Jethon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021628753&partnerID=40&md5=5edd5c3a8cccb091b90f81376149a569},
issn = {00446033},
year = {1984},
date = {1984-01-01},
journal = {Acta Physiologica Polonica},
volume = {35},
number = {2},
pages = {141-150},
abstract = {Wistar rats were used to study the effects of cernitins, i.e. aqueous and oil extracts or pollens, on the metabolic adaptation of the soleus muscle to exercise, vibration and raised ambient temperature. The animals were exposed to selected combinations of these factors for 5 days during 1.5 hour daily. A part of the animals was given orally cernitins in daily doses of 6 mg/kg of body weight for 10 days before the exposure. Among the adaptation changes studied in the soleus muscle, 24 hours after the last exposure, cernitins caused: 1) a reduction of the amount of total protein, 2) an increase in the proportion of soluble proteins in the protein fraction, 3) an increase in the tissue oxygen consumption, 4) an increase of already elevated pyruvate kinase activity, 5) a further rise in ATP level, 6) an increase in lactic dehydrogenase activity, 7) a rise in the activity of cholinesterases. Moreover, it increased significantly the body weight and the weight of the studied soleus muscle. Cernitins, in combination with certain types of exposure used in this experiment, exerted a catabolic action, increased the rate of anaerobic metabolism and enhanced adaptation to exercise, vibration and temperature. The direction of the adaptation changes depended on the type of exposure to environmental factors.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Wistar rats were used to study the effects of cernitins, i.e. aqueous and oil extracts or pollens, on the metabolic adaptation of the soleus muscle to exercise, vibration and raised ambient temperature. The animals were exposed to selected combinations of these factors for 5 days during 1.5 hour daily. A part of the animals was given orally cernitins in daily doses of 6 mg/kg of body weight for 10 days before the exposure. Among the adaptation changes studied in the soleus muscle, 24 hours after the last exposure, cernitins caused: 1) a reduction of the amount of total protein, 2) an increase in the proportion of soluble proteins in the protein fraction, 3) an increase in the tissue oxygen consumption, 4) an increase of already elevated pyruvate kinase activity, 5) a further rise in ATP level, 6) an increase in lactic dehydrogenase activity, 7) a rise in the activity of cholinesterases. Moreover, it increased significantly the body weight and the weight of the studied soleus muscle. Cernitins, in combination with certain types of exposure used in this experiment, exerted a catabolic action, increased the rate of anaerobic metabolism and enhanced adaptation to exercise, vibration and temperature. The direction of the adaptation changes depended on the type of exposure to environmental factors.