@article{2-s2.0-85173652699,

title = {Molecular mechanisms of SNAC1 (Stress-responsive NAC1) in conferring the abiotic stress tolerance},

author = { M.M. Kurowska and A. Daszkowska-Golec},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85173652699&doi=10.1016%2fj.plantsci.2023.111894&partnerID=40&md5=d43a797e2ba83efab5adf3b1cf3685e5},

doi = {10.1016/j.plantsci.2023.111894},

issn = {01689452},

year  = {2023},

date = {2023-01-01},

journal = {Plant Science},

volume = {337},

publisher = {Elsevier Ireland Ltd},

abstract = {NAC family gene - SNAC1 (Stress-responsive NAC1) is responsive to drought, salt, cold stress, and ABA. It acts as a regulator in mediating tolerance to abiotic stress through different pathways. Abiotic stress, among them drought and salinity, are adverse factors for plant growth and crop productivity. SNAC1 was an object of high interest according to the effect of improved drought and salt tolerance when overexpressed in different plant species such as rice, wheat, barley, cotton, maize, banana, or oat. SNAC1 functions by regulating the expression of genes that contain the NAC Recognized Sequence (NACRS) within their promoter region. This gene is induced by drought, specifically in guard cells. Its downstream targets have been identified. The role of SNAC1 in molecular and physiological responses during abiotic stress has been proposed, but this knowledge still needs to be expanded. Here, we describe recent advances in understanding the action of SNAC1 in adapting plants to abiotic stress. © 2023},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85169780516,

title = {Is it the end of TILLING era in plant science?},

author = { M.E. Szurman-Zubrzycka and M.M. Kurowska and B.J. Till and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85169780516&doi=10.3389%2ffpls.2023.1160695&partnerID=40&md5=459459f903d6d3b59fe1b9d34e88879f},

doi = {10.3389/fpls.2023.1160695},

issn = {1664462X},

year  = {2023},

date = {2023-01-01},

journal = {Frontiers in Plant Science},

volume = {14},

publisher = {Frontiers Media SA},

abstract = {Since its introduction in 2000, the TILLING strategy has been widely used in plant research to create novel genetic diversity. TILLING is based on chemical or physical mutagenesis followed by the rapid identification of mutations within genes of interest. TILLING mutants may be used for functional analysis of genes and being nontransgenic, they may be directly used in pre-breeding programs. Nevertheless, classical mutagenesis is a random process, giving rise to mutations all over the genome. Therefore TILLING mutants carry background mutations, some of which may affect the phenotype and should be eliminated, which is often time-consuming. Recently, new strategies of targeted genome editing, including CRISPR/Cas9-based methods, have been developed and optimized for many plant species. These methods precisely target only genes of interest and produce very few off-targets. Thus, the question arises: is it the end of TILLING era in plant studies? In this review, we recap the basics of the TILLING strategy, summarize the current status of plant TILLING research and present recent TILLING achievements. Based on these reports, we conclude that TILLING still plays an important role in plant research as a valuable tool for generating genetic variation for genomics and breeding projects. Copyright © 2023 Szurman-Zubrzycka, Kurowska, Till and Szarejko.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85103402126,

title = {Aquaporins in cereals-important players in maintaining cell homeostasis under abiotic stress},

author = { M.M. Kurowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103402126&doi=10.3390%2fgenes12040477&partnerID=40&md5=76918181776f36a735e943ce4f948da8},

doi = {10.3390/genes12040477},

issn = {20734425},

year  = {2021},

date = {2021-01-01},

journal = {Genes},

volume = {12},

number = {4},

publisher = {MDPI AG},

abstract = {Cereal productivity is reduced by environmental stresses such as drought, heat, elevated CO2, salinity, metal toxicity and cold. Sometimes, plants are exposed to multiple stresses simultaneously. Plants must be able to make a rapid and adequate response to these environmental stimuli in order to restore their growing ability. The latest research has shown that aquaporins are important players in maintaining cell homeostasis under abiotic stress. Aquaporins are membrane intrinsic proteins (MIP) that form pores in the cellular membranes, which facilitate the movement of water and many other molecules such as ammonia, urea, CO2, micronutrients (silicon and boron), glycerol and reactive oxygen species (hydrogen peroxide) across the cell and intercellular compartments. The present review primarily focuses on the diversity of aquaporins in cereal species, their cellular and subcellular localisation, their expression and their functioning under abiotic stresses. Lastly, this review discusses the potential use of mutants and plants that overexpress the aquaporin-encoding genes to improve their tolerance to abiotic stress. © 2021 by the author. Licensee MDPI, Basel, Switzerland.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85089435133,

title = {Barley ABI5 (Abscisic Acid INSENSITIVE 5) Is Involved in Abscisic Acid-Dependent Drought Response},

author = { A. Collin and A. Daszkowska-Golec and M.M. Kurowska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089435133&doi=10.3389%2ffpls.2020.01138&partnerID=40&md5=ab2494c5d3f9cd41dd76be0da41911a1},

doi = {10.3389/fpls.2020.01138},

issn = {1664462X},

year  = {2020},

date = {2020-01-01},

journal = {Frontiers in Plant Science},

volume = {11},

publisher = {Frontiers Media S.A.},

abstract = {ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) network and is activated in response to abiotic stresses. However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its function under stress remains elusive. Here, we show that HvABI5 is involved in ABA-dependent regulation of barley response to drought stress. We identified barley TILLING mutants carrying different alleles in the HvABI5 gene and we studied in detail the physiological and molecular response to drought and ABA for one of them. The hvabi5.d mutant, carrying G1751A transition, was insensitive to ABA during seed germination, yet it showed the ability to store more water than its parent cv. “Sebastian” (WT) in response to drought stress. The drought-tolerant phenotype of hvabi5.d was associated with better membrane protection, higher flavonoid content, and faster stomatal closure in the mutant under stress compared to the WT. The microarray transcriptome analysis revealed up-regulation of genes associated with cell protection mechanisms in the mutant. Furthermore, HvABI5 target genes: HVA1 and HVA22 showed higher activity after drought, which may imply better adaptation of hvabi5.d to stress. On the other hand, chlorophyll content in hvabi5.d was lower than in WT, which was associated with decreased photosynthesis efficiency observed in the mutant after drought treatment. To verify that HvABI5 acts in the ABA-dependent manner we analyzed expression of selected genes related to ABA pathway in hvabi5.d and its WT parent after drought and ABA treatments. The expression of key genes involved in ABA metabolism and signaling differed in the mutant and the WT under stress. Drought-induced increase of expression of HvNCED1, HvBG8, HvSnRK2.1, and HvPP2C4 genes was 2–20 times higher in hvabi5.d compared to “Sebastian”. We also observed a faster stomatal closure in hvabi5.d and much higher induction of HvNCED1 and HvSnRK2.1 genes after ABA treatment. Together, these findings demonstrate that HvABI5 plays a role in regulation of drought response in barley and suggest that HvABI5 might be engaged in the fine tuning of ABA signaling by a feedback regulation between biosynthetic and signaling events. In addition, they point to different mechanisms of HvABI5 action in regulating drought response and seed germination in barley. © Copyright © 2020 Collin, Daszkowska-Golec, Kurowska and Szarejko.},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85086678590,

title = {Methyl jasmonate affects photosynthesis efficiency, expression of HvTIP genes and nitrogen homeostasis in Barley},

author = { M.M. Kurowska and A. Daszkowska-Golec and M. Gajecka and P. Kościelniak and W.M. Bierza and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086678590&doi=10.3390%2fijms21124335&partnerID=40&md5=388ee2567db937bac0ac87ab71487383},

doi = {10.3390/ijms21124335},

issn = {16616596},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Molecular Sciences},

volume = {21},

number = {12},

pages = {1-23},

publisher = {MDPI AG},

abstract = {Jasmonates modulate many growth and developmental processes and act as stress hormones that play an important role in plant tolerance to biotic and abiotic stresses. Therefore, there is a need to identify the genes that are regulated through the jasmonate signalling pathway. Aquaporins, and among them the Tonoplast Intrinsic Proteins (TIPs), form the channels in cell membranes that are responsible for the precise regulation of the movement of water and other substrates between cell compartments. We identified the cis-regulatory motifs for the methyl jasmonate (MeJA)-induced genes in the promoter regions of all the HvTIP genes, which are active in barley seedlings, and thus we hypothesised that the HvTIP expression could be a response to jasmonate signalling. In the presented study, we determined the effect of methyl jasmonate on the growth parameters and photosynthesis efficiency of barley seedlings that had been exposed to different doses of MeJA (15–1000 µM × 120 h) in a hydroponic solution. All of the applied MeJA concentrations caused a significant reduction of barley seedling growth, which was most evident in the length of the first leaf sheath and dry leaf weight. The observed decrease of the PSII parameters after the exposure to high doses of MeJA (500 µM or higher) was associated with the downregulation of HvPsbR gene encoding one of the extrinsic proteins of the Oxygen Evolving Complex. The reduced expression of HvPsbR might lead to the impairment of the OEC action, manifested by the occurrence of the K-band in an analysis of fluorescence kinetics after MeJA treatment as well as reduced photosynthesis efficiency. Furthermore, methyl jasmonate treatment caused a decrease in the nitrogen content in barley leaves, which was associated with an increased expression the four tonoplast aquaporin genes (HvTIP1;2; HvTIP2;2; HvTIP4;1 and HvTIP4;2) predicted to transport the nitrogen compounds from the vacuole to the cytosol. The upregulation of the nitrogen-transporting HvTIPs might suggest their involvement in the vacuolar unloading of ammonia and urea, which both could be remobilised when the nitrogen content in the leaves decreases. Our research provides tips on physiological role of the individual TIP subfamily members of aquaporins under methyl jasmonate action. © 2020 by the authors.Licensee MDPI, Basel, Switzerland.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85076702349,

title = {Drought stress and re-watering affect the abundance of TIP aquaporin transcripts in barley},

author = { M.M. Kurowska and K. Wiecha and K. Gajek and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076702349&doi=10.1371%2fjournal.pone.0226423&partnerID=40&md5=42b8a9dfdb4d4118b9ae9f4f914d17ac},

doi = {10.1371/journal.pone.0226423},

issn = {19326203},

year  = {2019},

date = {2019-01-01},

journal = {PLoS ONE},

volume = {14},

number = {12},

publisher = {Public Library of Science},

abstract = {Tonoplast Intrinsic Proteins (TIP) are plant aquaporins that are primarily localized in the tonoplast and play a role in the bidirectional flux of water and other substrates across a membrane. In barley, eleven members of the HvTIP gene subfamily have been identified. Here, we describe the transcription profile of the HvTIP genes in the leaves of barley seedlings being grown under optimal moisture conditions, drought stress and a re-watering phase. The applied drought stress caused a 55% decrease in the relative water content (RWC) in seedlings, while re-watering increased the RWC to 90% of the control. Our analysis showed that all HvTIP genes, except HvTIP3;2, HvTIP4;3 and HvTIP5.1, were expressed in leaves of ten-day-old barley seedlings under optimal water conditions with the transcripts of HvTIP2;3, HvTIP1;2 and HvTIP1;1 being the most abundant. We showed, for the first time in barley, a significant variation in the transcriptional activity between the analysed genes under drought stress. After drought treatment, five HvTIP genes, which are engaged in water transport, were down-regulated to varying degrees, while two, HvTIP3;1 and HvTIP4;1, were up-regulated. The HvTIP3;1 isoform, which is postulated as transporting hydrogen peroxide, expressed the highest increase of activity (ca. 5000x) under drought stress, thus indicating its importance in the response to this stress. Re-hydration caused the return of the expression of many genes to the level that was observed under optimal moisture conditions or, at least, a change in this direction Additionally, we examined the promotor regions of HvTIP and detected the presence of the cis-regulatory elements that are connected with the hormone and stress responses in all of the genes. Overall, our results suggest that 7 of 11 studied HvTIP (HvTIP1;1; HvTIP1;2; HvTIP2;1; HvTIP2;2; HvTIP2;3; HvTIP3;1; HvTIP4;1) have an important function during the adaptation of barley to drought stress conditions. We discuss the identified drought-responsive HvTIP in terms of their function in the adaptation of barley to this stress. Copyright: © 2019 Kurowska et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85040322145,

title = {Mutation in barley ERA1 (Enhanced Response to ABA1) gene confers better photosynthesis efficiency in response to drought as revealed by transcriptomic and physiological analysis},

author = { A. Daszkowska-Golec and A. Collin and K. Sitko and M. Słota and M.M. Kurowska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040322145&doi=10.1016%2fj.envexpbot.2018.01.003&partnerID=40&md5=0007d532bbcbed5056c705585b2fd044},

doi = {10.1016/j.envexpbot.2018.01.003},

issn = {00988472},

year  = {2018},

date = {2018-01-01},

journal = {Environmental and Experimental Botany},

volume = {148},

pages = {12-26},

publisher = {Elsevier B.V.},

abstract = {Farnesylation is a post-translational modification that promotes the interaction between the modified signaling protein and membrane lipids and/or other proteins. Farnesyltransferase is the crucial enzyme involved in this process. Strikingly, plant mutants in the ERA1 (Enhanced response to ABA 1) gene, encoding β-subunit of farnesyltransferase, exhibited ABA-hypersensitivity during seed germination and drought tolerance in several species including Arabidopsis, wheat and soybean. However, the mechanism of ERA1 action has not been resolved yet. Here, we present the potential regulatory role of ERA1 in the drought signaling network in barley. With the aim of decoding the role of the ERA1 gene, we developed a unique barley mutant using TILLING analysis. Mutation in HvERA1 confers semi-dwarf phenotype, ABA-sensitivity during seed germination and drought tolerance. Our transcriptomic analysis suggested a role of HvERA1 in regulation of the crosstalk between ABA and ethylene at the onset of drought. Furthermore, analysis of hvera1.b response to prolonged drought stress linked HvERA1 to the metabolism of galactolipids, that build the chloroplast membranes. It might results in the protection of hvera1.b photosystem and thus, in its better photosynthesis performance under water stress. Together, these results indicate the possible mechanism of the primary cause of the observed alterations in the hvera1.b mutant. © 2018 Elsevier B.V.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85043326007,

title = {HorTILLUS—a rich and renewable source of induced mutations for forward/reverse genetics and pre-breeding programs in barley (Hordeumvulgare L.)},

author = { M.E. Szurman-Zubrzycka and J. Zbieszczyk and M. Marzec and J. Jelonek and B. Chmielewska and M.M. Kurowska and M. Krok and A. Daszkowska-Golec and J. Guzy-Wróbelska and D. Gruszka and M. Gajecka and P. Gajewska and M. Stolarek and P. Tylec and P. Sega and S. Lip and M. Kudełko and M. Lorek and M. Gorniak-Walas and A. Malolepszy and N. Podsiadlo and K.P. Szyrajew and A. Keisa and Z. Mbambo and E. Todorowska and M. Gaj and Z. Nita and W. Orlowska-Job and M. Maluszynski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043326007&doi=10.3389%2ffpls.2018.00216&partnerID=40&md5=2be0ee225baa473124ed786053f7b1c1},

doi = {10.3389/fpls.2018.00216},

issn = {1664462X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Plant Science},

volume = {9},

publisher = {Frontiers Media S.A.},

abstract = {TILLING (Targeting Induced Local Lesions IN Genomes) is a strategy used for functional analysis of genes that combines the classical mutagenesis and a rapid, high-throughput identification of mutations within a gene of interest. TILLING has been initially developed as a discovery platform for functional genomics, but soon it has become a valuable tool in development of desired alleles for crop breeding, alternative to transgenic approach. Here we present the HorTILLUS (Hordeum—TILLING—University of Silesia) population created for spring barley cultivar “Sebastian” after double-treatment of seeds with two chemical mutagens: sodium azide (NaN3) and N-methyl-N-nitrosourea (MNU). The population comprises more than 9,600 M2 plants from which DNA was isolated, seeds harvested, vacuum-packed, and deposited in seed bank. M3 progeny of 3,481 M2 individuals was grown in the field and phenotyped. The screening for mutations was performed for 32 genes related to different aspects of plant growth and development. For each gene fragment, 3,072–6,912 M2 plants were used for mutation identification using LI-COR sequencer. In total, 382 mutations were found in 182.2Mb screened. The average mutation density in the HorTILLUS, estimated as 1 mutation per 477kb, is among the highest mutation densities reported for barley. The majority of mutations were G/C to A/T transitions, however about 8% transversions were also detected. Sixty-one percent of mutations found in coding regions were missense, 37.5% silent and 1.1% nonsense. In each gene, the missense mutations with a potential effect on protein function were identified. The HorTILLUS platform is the largest of the TILLING populations reported for barley and best characterized. The population proved to be a useful tool, both in functional genomic studies and in forward selection of barley mutants with required phenotypic changes. We are constantly renewing the HorTILLUS population, which makes it a permanent source of new mutations. We offer the usage of this valuable resource to the interested barley researchers on cooperative basis. © 2018 Szurman-Zubrzycka, Zbieszczyk, Marzec, Jelonek, Chmielewska, Kurowska, Krok, Daszkowska-Golec, Guzy-Wrobelska, Gruszka, Gajecka, Gajewska, Stolarek, Tylec, Sega, Lip, Kudełko, Lorek, Gorniak-Walas, Malolepszy, Podsiadlo, Szyrajew, Keisa, Mbambo, Todorowska, Gaj, Nita, Orlowska-Job, Maluszynski and Szarejko.},

note = {30},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021152716,

title = {Mutation in HvCBP20 (Cap binding protein 20) adapts barley to drought stress at phenotypic and transcriptomic levels},

author = { A. Daszkowska-Golec and A. Collin and M. Marzec and M. Słota and M.M. Kurowska and M. Gajecka and P. Gajewska and T. Płociniczak and K. Sitko and A. Pacak and Z. Szweykowska-Kulinska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021152716&doi=10.3389%2ffpls.2017.00942&partnerID=40&md5=769fb24d1f52b6fc895f399773d7ea69},

doi = {10.3389/fpls.2017.00942},

issn = {1664462X},

year  = {2017},

date = {2017-01-01},

journal = {Frontiers in Plant Science},

volume = {8},

publisher = {Frontiers Media S.A.},

abstract = {CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the HvCBP20 gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the hvcbp20.ab mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the hvcbp20.ab mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of hvcbp20.ab mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response. © 2017 Daszkowska-Golec, Skubacz, Marzec, Slota, Kurowska, Gajecka, Gajewska, Płociniczak, Sitko, Pacak, Szweykowska-Kulinska and Szarejko.},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85008234694,

title = {Barley primary microRNA expression pattern is affected by soil water availability},

author = { A. Swida-Barteczka and K. Kruszka and A. Grabowska and A. Pacak and A. Jarmolowski and M.M. Kurowska and I. Szarejko and Z. Szweykowska-Kulinska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008234694&doi=10.18388%2fabp.2016_1352&partnerID=40&md5=87a73c5e5bbb97a2759b489fb7443f7a},

doi = {10.18388/abp.2016_1352},

issn = {0001527X},

year  = {2016},

date = {2016-01-01},

journal = {Acta Biochimica Polonica},

volume = {63},

number = {4},

pages = {817-824},

publisher = {Polskie Towarzystwo Biochemiczne},

abstract = {MicroRNAs are short molecules of 21-24 nt in length. They are present in all eukaryotic organisms and regulate gene expression by guiding posttranscriptional silencing of mRNAs. In plants, they are key players in signal transduction, growth and development, and in response to abiotic and biotic stresses. Barley (Hordeum vulgare) is an economically important monocotyledonous crop plant. Drought is the world's main cause of loss in cereal production. We have constructed a high-throughput Real-Time RT-qPCR platform for parallel determination of 159 barley primary microRNAs' levels. The platform was tested for two drought-and-rehydration-treated barley genotypes (Rolap and Sebastian). We have determined changes in the expression of primary microRNAs responding to mild drought, severe drought, and rehydration. Based on the results obtained, we conclude that alteration in the primary microRNA expression is relative to the stress's intensity. Mild drought and rehydration mostly decrease the pri-miRNA levels in both of the tested genotypes. Severe drought mainly induces the primary microRNA expression. The main difference between the genotypes tested was a much-stronger induction of pri-miRNAs in Rolap encountering severe drought. The primary microRNAs respond dynamically to mild drought, severe drought, and rehydration treatments. We propose that some of the individual pri-miRNAs could be used as drought stress or rehydration markers. The usage of the platform in biotechnology is also postulated.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84924084540,

title = {Invitro-propagated Miscanthus×giganteus plants can be a source of diversity in terms of their chemical composition},

author = { A. Płazek and F. Dubert and P. Kopeć and T. Krepski and P. Kacorzyk and P. Micek and M.M. Kurowska and I. Szarejko and G. Zurek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924084540&doi=10.1016%2fj.biombioe.2015.02.009&partnerID=40&md5=e74886591a545d3b69f9b389c0449570},

doi = {10.1016/j.biombioe.2015.02.009},

issn = {09619534},

year  = {2015},

date = {2015-01-01},

journal = {Biomass and Bioenergy},

volume = {75},

pages = {142-149},

publisher = {Elsevier Ltd},

abstract = {Miscanthus×. giganteus is a triploid hybrid propagated vegetatively from rhizomes or in a tissue culture, so its clones are characterised by a very low genetic diversity. Giant Miscanthus is cultivated mainly for biomass used as biofuel; depending on its chemical composition, it can be used in many industries. The goal of this work was to determine whether regenerants obtained in tissue culture can be a source of new forms characterised by different biomass yield and energy values as well as by their concentration of cellulose, hemicellulose and lignin. In the study the number and height of stems as well as the dry weight, ash and water mass fraction of biomass were estimated. The study was performed on Miscanthus plants propagated from rhizomes obtained from two different locations in Poland (Zabierzów - Z and Radzików - R), and on regenerants (Iv) obtained from Z plants. The results indicated that somaclonal variation occurs in M.×. giganteus cultures and that regenerants show low polymorphic variation compared to donor plants. Plants propagated in an invitro culture showed the lowest stem height, but the highest number of stems per plant. Dry matter yield was greatest in the R group, while regenerants demonstrated the highest level of hemicellulose fraction and lowest cellulose and lignin concentration. Energy value was the highest for Z plants, but Iv plants showed energy values on the same level as those of R plants. The data suggest that invitro-propagated plants may be a source of new Miscanthus forms. © 2015 Elsevier Ltd.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84914146005,

title = {Induced variations in brassinosteroid genes define barley height and sturdiness, and expand the green revolution genetic toolkit},

author = { C. Dockter and D. Gruszka and I. Braumann and A. Druka and I. Druka and J. Franckowiak and S.P. Gough and A. Janeczko and M.M. Kurowska and J. Lundqvist and U. Lundqvist and M. Marzec and I. Matyszczak and A.H. Müller and J. Oklestkova and B. Schulz and S. Zakhrabekova and M. Hansson},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84914146005&doi=10.1104%2fpp.114.250738&partnerID=40&md5=f95e254bcf209d3c56eb585f06883b42},

doi = {10.1104/pp.114.250738},

issn = {00320889},

year  = {2014},

date = {2014-01-01},

journal = {Plant Physiology},

volume = {166},

number = {4},

pages = {1912-1927},

publisher = {American Society of Plant Biologists},

abstract = {Reduced plant height and culm robustness are quantitative characteristics important for assuring cereal crop yield and quality under adverse weather conditions. A very limited number of short-culm mutant alleles were introduced into commercial crop cultivars during the Green Revolution. We identified phenotypic traits, including sturdy culm, specific for deficiencies in brassinosteroid biosynthesis and signaling in semidwarf mutants of barley (Hordeum vulgare). This set of characteristic traits was explored to perform a phenotypic screen of near-isogenic short-culm mutant lines from the brachytic, breviaristatum, dense spike, erectoides, semibrachytic, semidwarf, and slender dwarf mutant groups. In silico mapping of brassinosteroid-related genes in the barley genome in combination with sequencing of barley mutant lines assigned more than 20 historic mutants to three brassinosteroid-biosynthesis genes (BRASSINOSTEROID-6-OXIDASE; CONSTITUTIVE PHOTOMORPHOGENIC DWARF; and DIMINUTO) and one brassinosteroid-signaling gene (BRASSINOSTEROIDINSENSITIVE1 [HvBRI1]). Analyses of F2 and M2 populations, allelic crosses, and modeling of nonsynonymous amino acid exchanges in protein crystal structures gave a further understanding of the control of barley plant architecture and sturdiness by brassinosteroidrelated genes. Alternatives to the widely used but highly temperature-sensitive uzu1.a allele of HvBRI1 represent potential genetic building blocks for breeding strategies with sturdy and climate-tolerant barley cultivars. © 2014 American Society of Plant Biologists. All rights reserved.},

note = {81},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84882386220,

title = {Catalytic turnover triggers exchange of subunits of the magnesium chelatase AAA+ motor unit},

author = { J. Lundqvist and I. Braumann and M.M. Kurowska and A.H. Müller and M. Hansson},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84882386220&doi=10.1074%2fjbc.M113.480012&partnerID=40&md5=32df7ef57896d67e24f502ab30069151},

doi = {10.1074/jbc.M113.480012},

issn = {00219258},

year  = {2013},

date = {2013-01-01},

journal = {Journal of Biological Chemistry},

volume = {288},

number = {33},

pages = {24012-24019},

abstract = {Background: Magnesium chelatase is an AAA+ protein complex involved in chlorophyll biosynthesis. Results: An exchange of subunits occurs during the catalytic cycle. Conclusion: Dissociation of the complex may be part of the reaction mechanism. Significance: Deciphering of the mechanism of AAA+protein complexes is crucial for our understanding of the catalytic cycle of a common class of molecular machines. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84865992818,

title = {Unexpected genetic diversity of Fallopia japonica from Central Europe revealed after AFLP analysis},

author = { K. Bzdęga and A. Janiak and S. Tarłowska and M.M. Kurowska and B. Tokarska-Guzik and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865992818&doi=10.1016%2fj.flora.2012.05.002&partnerID=40&md5=6fae375b33865dfffbbe0280848f867c},

doi = {10.1016/j.flora.2012.05.002},

issn = {03672530},

year  = {2012},

date = {2012-01-01},

journal = {Flora: Morphology, Distribution, Functional Ecology of Plants},

volume = {207},

number = {9},

pages = {636-645},

abstract = {Recently much attention has been paid to genetic aspects of invasive success in Japanese knotweed s.l. One hypothesis to explain the invasive spread of these species is a multiple introduction, which leads to a higher level of genetic diversity in the invaded range. Fallopia japonica is considered to be genetically uniform in Europe, introduced as a single female clone. However, there is some evidence suggesting that invasion history and dynamics differ between Western and Central-Eastern Europe. We used AFLP markers to characterize genetic diversity of three Fallopia taxa that occur in Poland: F. japonica, F. sachalinensis and their hybrid Fallopia×. bohemica, growing in so-called 'homogeneous' populations, consisting of one taxon and 'heterogeneous' populations, composed of the three taxa cohabiting together. No polymorphism, resp. an insignificantly low variability was observed in the 'homogeneous' populations. In the 'heterogeneous' stands polymorphism was detected within each taxa, with one exception that concerns individuals of F. sachalinensis from a riparian habitat. The highest level of polymorphism was found among individuals of F.×. bohemica. The most striking result of our study is the observation of polymorphism between individuals of F. japonica. The AFLP data also showed that F.×. bohemica is most diverse when occurring in a heterogeneous configuration with F. japonica and F. sachalinensis in the same habitat. Our results are the first evidence of genetic diversity in F. japonica populations in Central Europe and can implicate the possibility of its multiple introduction in this region or the existence of sexual reproduction of this species. © 2012 .},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84864761600,

title = {Genetic diversity of populations of Brachypodium pinnatum (L.) P. Beauv.: Expansive grass in a fragmented landscape},

author = { W. Bąba and M.M. Kurowska and A. Kompała-Bąba and A. Wilczek and J. Długosz and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864761600&partnerID=40&md5=77be6883438e6d82ef961500eec698c8},

issn = {15052249},

year  = {2012},

date = {2012-01-01},

journal = {Polish Journal of Ecology},

volume = {60},

number = {1},

pages = {31-40},

abstract = {Brachypodium pinnatum belongs to native grasses which could dramatically decrease the biodiversity of calcareous grasslands, therefore, an examination of the mechanisms of its expansion is of prime importance for their conservation. We studied the genetic structure of 12 subpopulations of the expansive grass B. pinnatum in a heterogeneous landscape with AFLP markers, aiming at determination whether spatial isolation influences the genetic diversity and the population genetic structure of this species. A high level of overall (79%) and within population polymorphic loci (38-44%) were found and 220 different genotypes were distinguished among the 252 samples analysed. No significant population structure nor isolation-bydistance were found, despite their long-time fragmentation history. This confirms that isolation of calcareous grasslands in a landscape cannot prevent the expansion of B. pinnatum grasses because of the high generative dispersal ability. Subsequent quick and extensive clonal growth of this species enables the successful establishment. In most cases mowing or grazing is sufficient to keep this species at a low density within ancient grasslands and to prevent seed and pollen dispersal, but not for restoration of species-rich calcareous grasslands.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84861191576,

title = {Genetic diversity of the expansive grass Brachypodium pinnatum in a changing landscape: Effect of habitat age},

author = { W. Bąba and M.M. Kurowska and A. Kompała-Bąba and A. Wilczek and J. Długosz and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861191576&doi=10.1016%2fj.flora.2012.01.011&partnerID=40&md5=5a6606fb56f917d5a3822dcd8c4cafd7},

doi = {10.1016/j.flora.2012.01.011},

issn = {03672530},

year  = {2012},

date = {2012-01-01},

journal = {Flora: Morphology, Distribution, Functional Ecology of Plants},

volume = {207},

number = {5},

pages = {346-353},

abstract = {Perennial grasses constitute a major group of species showing a dramatic decline of biodiversity in successional plant communities. Using AFLP markers, we examined 12 populations of the expansive grass . Brachypodium pinnatum differing in habitat age (30-50; ca. 100 and >300 years old) in order to determine whether clonal diversity of populations, genetic variation, and the relative importance of clonal propagation versus sexual reproduction change with grassland age. Five AFLP primer combinations gave a total of 517 bands, 79% of which were polymorphic. 314 different multilocus lineages were distinguished among the 453 samples analyzed. The number of genotypes (. G) and clonal richness (. R) decreased with habitat age, while the distribution of the frequency of genets changed from many clones of similar size to dominance by one or a few large clones. We consider these results to give evidence of significant role of sexual reproduction in the early phases of colonization and prevalence of clonal growth and competitive exclusion of less adapted genotypes in the later ones. However, habitat age had only marginal effect on genetic diversity, as percentage of polymorphic loci (. PPL) within all the populations analyzed was similar, viz. 38.6-43.5%. © 2012 Elsevier GmbH.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84858173210,

title = {Induced mutations in circadian clock regulator Mat-a facilitated short-season adaptation and range extension in cultivated barley},

author = { S. Zakhrabekova and S.P. Gough and I. Braumann and A.H. Müller and J. Lundqvist and K. Ahmann and C. Dockter and I. Matyszczak and M.M. Kurowska and A. Druka and R. Waugh and A. Granerd and N. Stein and B. Steuernagel and U. Lundqvist and M. Hansson},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84858173210&doi=10.1073%2fpnas.1113009109&partnerID=40&md5=00154d59941bf7ab3fca7df90313e8b6},

doi = {10.1073/pnas.1113009109},

issn = {00278424},

year  = {2012},

date = {2012-01-01},

journal = {Proceedings of the National Academy of Sciences of the United States of America},

volume = {109},

number = {11},

pages = {4326-4331},

abstract = {Time to flowering has an important impact on yield and has been a key trait in the domestication of crop plants and the spread of agriculture. In 1961, the cultivar Mari (mat-a.8) was the very first induced early barley (Hordeum vulgare L.) mutant to be released into commercial production. Mari extended the range of two-row spring barley cultivation as a result of its photoperiod insensitivity. Since its release, Mari or its derivatives have been used extensively across the world to facilitate short-season adaptation and further geographic range extension. By exploiting an extended historical collection of early-flowering mutants of barley, we identified Praematurum-a (Mat-a), the gene responsible for this key adaptive phenotype, as a homolog of the Arabidopsis thaliana circadian clock regulator Early Flowering 3 (Elf3). We characterized 87 induced mat-a mutant lines and identified >20 different mata alleles that had clear mutations leading to a defective putative ELF3 protein. Expression analysis of HvElf3 and Gigantea in mutant and wild-type plants demonstrated that mat-a mutations disturb the flowering pathway, leading to the early phenotype. Alleles of Mat-a therefore have important and demonstrated breeding value in barley but probably also in many other daylength- sensitive crop plants, where they may tune adaptation to different geographic regions and climatic conditions, a critical issue in times of global warming.},

note = {105},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84872271745,

title = {Molecular analysis of point mutations in a barley genome exposed to MNU and Gamma rays},

author = { M.M. Kurowska and A. Labocha-Pawłowska and D. Gnizda and M. Maluszynski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872271745&doi=10.1016%2fj.mrfmmm.2012.08.008&partnerID=40&md5=851b772953f00b9b480da6f446be515f},

doi = {10.1016/j.mrfmmm.2012.08.008},

issn = {00275107},

year  = {2012},

date = {2012-01-01},

journal = {Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis},

volume = {738-739},

number = {1},

pages = {52-70},

publisher = {Elsevier B.V.},

abstract = {We present studies aimed at determining the types and frequencies of mutations induced in the barley genome after treatment with chemical (N-methyl-N-nitrosourea; MNU) and physical (gamma rays) mutagens. We created M2 populations of a doubled haploid line and used them for the analysis of mutations in targeted DNA sequences and over an entire barley genome using TILLING (Targeting Induced Local Lesions in Genomes) and AFLP (Amplified Fragment Length Polymorphism) technique, respectively. Based on the TILLING analysis of the total DNA sequence of 4,537,117 bp in the MNU population, the average mutation density was estimated as 1/504 kb. Only one nucleotide change was found after an analysis of 3,207,444 bp derived from the highest dose of gamma rays applied. MNU was clearly a more efficient mutagen than gamma rays in inducing point mutations in barley. The majority (63.6%) of the MNU-induced nucleotide changes were transitions, with a similar number of G > A and C > T substitutions. The similar share of G > A and C > T transitions indicates a lack of bias in the repair of O6-methylguanine lesions between DNA strands. There was, however, a strong specificity of the nucleotide surrounding the O6-meG at the -1 position. Purines formed 81% of nucleotides observed at the -1 site. Scanning the barley genome with AFLP markers revealed ca. a three times higher level of AFLP polymorphism in MNUtreated as compared to the gamma-irradiated population. In order to check whether AFLP markers can really scan the whole barley genome for mutagen-induced polymorphism, 114 different AFLP products, were cloned and sequenced. 94% of bands were heterogenic, with some bands containing up to 8 different amplicons. The polymorphic AFLP products were characterised in terms of their similarity to the records deposited in a GenBank database. The types of sequences present in the polymorphic bands reflected the organisation of the barley genome. © 2012 Elsevier B.V.},

note = {36},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-80053627631,

title = {TILLING - a shortcut in functional genomics},

author = { M.M. Kurowska and A. Daszkowska-Golec and D. Gruszka and M. Marzec and M. Szurman and I. Szarejko and M. Maluszynski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80053627631&doi=10.1007%2fs13353-011-0061-1&partnerID=40&md5=59e48b59a6de3676dbabe53974f2599e},

doi = {10.1007/s13353-011-0061-1},

issn = {12341983},

year  = {2011},

date = {2011-01-01},

journal = {Journal of Applied Genetics},

volume = {52},

number = {4},

pages = {371-390},

abstract = {Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN Genomes) was developed a decade ago as an alternative to insertional mutagenesis. It takes advantage of classical mutagenesis, sequence availability and high-throughput screening for nucleotide polymorphisms in a targeted sequence. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of its genome size and ploidy level. The TILLING protocol provides a high frequency of point mutations distributed randomly in the genome. The great mutagenic potential of chemical agents to generate a high rate of nucleotide substitutions has been proven by the high density of mutations reported for TILLING populations in various plant species. For most of them, the analysis of several genes revealed 1 mutation/200-500 kb screened and much higher densities were observed for polyploid species, such as wheat. High-throughput TILLING permits the rapid and low-cost discovery of new alleles that are induced in plants. Several research centres have established a TILLING public service for various plant species. The recent trends in TILLING procedures rely on the diversification of bioinformatic tools, new methods of mutation detection, including mismatch-specific and sensitive endonucleases, but also various alternatives for LI-COR screening and single nucleotide polymorphism (SNP) discovery using next-generation sequencing technologies. The TILLING strategy has found numerous applications in functional genomics. Additionally, wide applications of this throughput method in basic and applied research have already been implemented through modifications of the original TILLING strategy, such as Ecotilling or Deletion TILLING. © 2011 The Author(s).},

note = {130},

keywords = {},

pubstate = {published},

tppubtype = {article}

}