@article{2-s2.0-85204007727,

title = {Assessing the toxicity of green Agaricus bisporus-based Cadmium Sulfide nanoparticles on Musca domestica as a biological model},

author = { H.I. Elhenawy and N.A. Toto and A.S. Eltaweil and H.K. Hussein and M. Augustyniak and L.M. El-Samad},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85204007727&doi=10.1038%2fs41598-024-70060-y&partnerID=40&md5=10d7ae2a0a4ac926cfdb190e7b46434f},

doi = {10.1038/s41598-024-70060-y},

year  = {2024},

date = {2024-01-01},

journal = {Scientific Reports},

volume = {14},

number = {1},

publisher = {Nature Research},

abstract = {The common housefly, Musca domestica, known for transmitting over 100 infections, was studied using green-synthesized Cadmium Sulfide nanoparticles (CdS NPs) from Agaricus bisporus. These CdS NPs were tested on third-instar larvae under laboratory conditions using dipping and feeding methods with concentrations (75; 100; 125; 150; 175; and 200 µg/mL). The toxicity, measured by LC50, was found to be 138 µg/mL for dipping treatment and 123 µg/mL for feeding treatment. Analysis with an energy-dispersive X-ray microanalyzer confirmed Cd accumulation in the larval midgut, indicating penetration of CdS NPs into the organism, which may potentially increase their toxicity. CdS NPs caused disruptions in Heat Shock Protein 70, cell apoptosis, and various biochemical components. Scanning electron microscopy revealed morphological abnormalities in larvae, pupae, and adults exposed to CdS NPs. Ultrastructural examination showed significant midgut tissue abnormalities in larvae treated with 123 µg/mL of CdS NPs. Our study demonstrated that green-synthesized CdS NPs from A. bisporus can effectively control the development of M. domestica larvae. © The Author(s) 2024.},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85207719711,

title = {Graphene oxide in low concentrations can change mitochondrial potential, autophagy, and apoptosis paths in two strains of invertebrates with different life strategies},

author = { B. Flasz and A. Babczyńska and M. Tarnawska and A.K. Ajay and A. Kędziorski and Ł. Napora-Rutkowski and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85207719711&doi=10.1016%2fj.bbrc.2024.150898&partnerID=40&md5=6f3f1c36eb8e21112703c71de163f5fd},

doi = {10.1016/j.bbrc.2024.150898},

year  = {2024},

date = {2024-01-01},

journal = {Biochemical and Biophysical Research Communications},

volume = {736},

publisher = {Elsevier B.V.},

abstract = {Nanoparticles, like graphene oxide (GO), are particles with unique physiochemical properties that enable their wide application in various areas of life. The effects of GO on individual cell organelles like mitochondria and the effects of interactions are worth investigating, as they can activate multiple cellular processes, such as autophagy or apoptosis. Mitochondrial injury plays an essential role in the majority of cell death routines. In the project, we investigated cell health status measured as mitochondrial inner membrane depolarization, autophagy, and apoptosis induction during long-term GO administration in food (0.02 μg g−1 and 0.2 μg g−1 of food). Two unique Acheta domesticus strains that differ in life strategy were used: wild-type and long-lived at three different life stages (larva; young adult; mature adult). The changes in mitochondrial trans-membrane potential were marked in the wild-type strain. The autophagy was lower in all GO-treated groups in both strains, and the apoptosis was lower in both strains in the mature adult crickets. Low GO concentrations treatment for the whole life, despite mitochondrial dysfunction, may lead to inhibition of autophagy and apoptosis by arresting the cell cycle for the duration of repair, and other repair tools are involved in the process of restoring homeostasis. © 2024 The Authors},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85205372886,

title = {Liquid chromatography–mass spectrometry profiling of propolis and royal jelly and their ameliorative effects on cadmium-instigated pathological consequences in ovarian tissues of rats},

author = { A. Amr and A.E. Abdel-Karim and A.A. Abd el-Wahed and H.R. El-Seedi and M. Augustyniak and A. El Wakil and L.M. El-Samad and M.A. Hassan},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85205372886&doi=10.1016%2fj.microc.2024.111800&partnerID=40&md5=4a80f62b4913cddec389edd2b8eb03ca},

doi = {10.1016/j.microc.2024.111800},

year  = {2024},

date = {2024-01-01},

journal = {Microchemical Journal},

volume = {207},

publisher = {Elsevier Inc.},

abstract = {This study was undertaken to identify the bioactive components of propolis (PRO) and royal jelly (ROJ) and their therapeutic influence on attenuating cadmium chloride (CdCl2)-induced ovarian toxicity and dysfunction in rats. The composition of PRO or ROJ was ascertained employing the liquid chromatography–linear ion trap quadrupole–mass spectrometry (LC–LTQ–MS–MS) method. To further evaluate the anti-infertility properties of PRO and ROJ instigated by cadmium (Cd) in vivo, thirty female mature rats (183.3 ± 12.5 g) were randomly assigned into six groups (n = 5 per group). The established animal groups included (I) control, (II) Cd, (III) PRO, (IV) ROJ, (VI) PRO + Cd, and (VII) ROJ + Cd groups. Cadmium exposure disrupted ovarian tissue functions, demonstrating remarkable disorders in progesterone and estrogen. Furthermore, the comet assay exhibited a marked rise in DNA impairment in Cd-exposed rats, implying genotoxicity. Conversely, PRO or ROJ significantly counteracted the Cd-instigated pathophysiological consequences, ameliorating hormonal levels and lessening DNA damage. Critically, histological analysis revealed several anomalies in the ovarian tissues of the Cd-administered group. Interestingly, PRO or ROJ preserved the typical structure of ovarian tissues. Our findings indicate that both PRO and ROJ could be utilized to attenuate the pernicious influences incited by Cd due to their anti-androgenic attributes. © 2024 Elsevier B.V.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85207694527,

title = {Adult young as the fragile ontogenetic stage of the house crickets dietary exposed to GO nanoparticles – digestive enzymes perspective},

author = { A. Babczyńska and M. Tarnawska and K. Czaja and B. Flasz and A.K. Ajay and Ł. Napora-Rutkowski and K. Rozpędek and E. Świerczek and A. Kędziorski and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85207694527&doi=10.1016%2fj.chemosphere.2024.143641&partnerID=40&md5=7bc3885c8c6f666f5934e8b1e602e553},

doi = {10.1016/j.chemosphere.2024.143641},

year  = {2024},

date = {2024-01-01},

journal = {Chemosphere},

volume = {367},

publisher = {Elsevier Ltd},

abstract = {Graphene oxide nanoparticles (GO) (have promising properties for; electronics; energy; medicine; water purification; agriculture and food production industry. However; their potentially hazardous effects are still not satisfactorily recognized; so they are often included in the group of contaminants of emerging concern. Therefore; the aim of this investigation was to assess the potentially harmful effects of orally administered GO on the digestive enzyme activities of the house crickets Acheta domesticus. The activity of digestive enzymes was measured using the API®ZYM test and the results were compared in relation to the insect age group (Larvae; Adult Young and Adult Mature) and previous selection towards longevity (‘long living’ vs ‘wild’ strains). General analysis; encompassing all digestive enzymes simultaneously; as well as an analysis performed for individual enzyme subgroups; revealed that both factors and their interactions significantly modified the activity of digestive enzymes of A. domesticus. The most pronounced effect was connected with the age factor. The study revealed that the Adult Young age group was the most sensitive; in this group; the activity of digestive enzymes was; in general; decreased in comparison with the control group; while the remaining group did not respond to GO with a similar intensity. In turn; the inter-strain comparisons demonstrated different response patterns to the GO. The control enzymatic activity of the insects from long living’ strain was; in general; higher than in the ‘wild strain’; however; it was significantly decreased in the GO-exposed groups; while in the ‘wild’ strain; the activity remained relatively homogenous. Additionally; the tendency was more pronounced in Adult Young than in the remaining age groups. The potential toxicity of GO requires further investigation; mainly with a focus on the composition and functioning of gut microbiotas. © 2024 The Authors},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85202595845,

title = {Impacts of Combining Steinernema carpocapsae and Bracon hebetor Parasitism on Galleria mellonella Larvae},

author = { N.A. Aamer and Z.A. El-Moaty and M. Augustyniak and L.M. El-Samad and H.S. Hussein},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85202595845&doi=10.3390%2finsects15080588&partnerID=40&md5=fb592870c4477ebc496b1ef6624c5959},

doi = {10.3390/insects15080588},

year  = {2024},

date = {2024-01-01},

journal = {Insects},

volume = {15},

number = {8},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {The greater wax moth, Galleria mellonella, is a significant pest in apiculture and a well-established model organism for immunological and ecotoxicological studies. This investigation explores the individual and combined effects of the ectoparasite Bracon hebetor (B.h.) and the entomopathogenic nematode Steinernema carpocapsae (S.c.) on G. mellonella larvae. We evaluated the activity of oxidative stress enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), malondialdehyde (MDA) levels, cytochrome P450 activity, cell viability using Annexin V-FITC, DNA damage via comet assay, and larval morphology through scanning electron microscopy (SEM). Control larvae exhibited higher GPx and GST activities compared to those treated with B.h., S.c., or the B.h. + S.c. combination. Conversely, MDA levels displayed the opposite trend. SOD activity was reduced in the B.h. and S.c. groups but significantly higher in the combined treatment. Cytochrome P450 activity increased in response to parasitism by B. hebetor. The Annexin V-FITC assay revealed decreased cell viability in parasitized groups (B.h. 79.4%; S.c. 77.3%; B.h. + S.c. 70.1%) compared to controls. DNA damage analysis demonstrated significant differences between groups, and SEM observations confirmed severe cuticle abnormalities or malformations in G. mellonella larvae. These findings highlight the complex interactions between B. hebetor, S. carpocapsae, and their host, G. mellonella. Additionally, they illuminate the intricate physiological responses triggered within the host larvae. © 2024 by the authors.},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85199806703,

title = {Correction to: Memory Impairment, Pro-Inflammatory Host Response and Brain Histopathologic Severity in Rats Infected with K. pneumoniae or P. aeruginosa Meningitis (Pathogens, (2022), 11, 8, (933), 10.3390/pathogens11080933)},

author = { B.H. Elwakil and B.A. Bakr and M.M. Aljeldah and N.S. Shehata and Y.H. Shahin and Z.A. Olama and M. Augustyniak and M.A.M. Aboul-Soud and A.E. Wakil},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85199806703&doi=10.3390%2fpathogens13070595&partnerID=40&md5=ed3a84527abb9f43f9236336618673c5},

doi = {10.3390/pathogens13070595},

year  = {2024},

date = {2024-01-01},

journal = {Pathogens},

volume = {13},

number = {7},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {In the original publication [1], there was a mistake in Figure 5 and Supplementary Figure S2A as published. There was an error in the sub-image of the first row, the fourth column the sub-image (B) in hippocampus day 5 in Figure 5. In Figure S2A, only change the value of granular layer thickness in the hippocampus on day 5. The corrected Figure 5 and Supplementary Figure S2A appear below. A photomicrograph showing the individual brain tissues at different time intervals of K. pneumoniae infected rats. In the cerebral cortex, (A) The control group showing normal cytoarchitecture with normal neurons; (B) Day 5 showing vacuolations (stars); (C) Day 10 showing dilated congested blood vessel (arrow) with perivascular edema and vacuolations (stars); (D) Day 15 showing dilated blood vessels (black arrows), severe congestion (green arrows), vacuolations (stars) and gliosis (red bent arrows), a large area of necrotic foci in the brain parenchyma along with lymphocyte infiltration and the presence of degenerating and/or apoptotic neurons (N). While in the hippocampus: (A) The control group showing normal cytoarchitecture; (B) Day 5 showing decreased thickness of the pyramidal layer (bracket); (C) Day 10 showing degeneration and vacuolation (stars); (D) Day 15 exhibited a number of vacuolations (stars). Supplementary Figure S2: The thickness of the pyramidal layer of the hippocampus at different time intervals of infected rats with either (A) K. pneumoniae, or (B) P. aeruginosa was assessed. Herein, five different fields in each photomicrograph from Figures 5 and 6 at each different time interval of infected rats were analyzed on Intel® Core I7® based computer using VideoTest Morphology® software (Russia) with a specific built-in routine for measuring the thickness of the pyramidal layer of the hippocampus. The difference between K. pneumoniae and P. aeruginosa in decreasing the thickness of the layer became more evident at day 15 after infection, and it is slightly higher in case of P. aeruginosa than K. pneumoniae. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated. © 2024 by the authors.},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85206533473,

title = {Ascorbic Acid and Graphene Oxide Exposure in the Model Organism Acheta domesticus Can Change the Reproduction Potential},

author = { B. Flasz and M. Tarnawska and A. Kędziorski and Ł. Napora-Rutkowski and J. Szczygieł and Ł. Gajda and N. Nowak and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85206533473&doi=10.3390%2fmolecules29194594&partnerID=40&md5=ccf5d71227752ccd2588c796a8379323},

doi = {10.3390/molecules29194594},

year  = {2024},

date = {2024-01-01},

journal = {Molecules},

volume = {29},

number = {19},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {The use of nanoparticles in the industry carries the risk of their release into the environment. Based on the presumption that the primary graphene oxide (GO) toxicity mechanism is reactive oxygen species production in the cell, the question arises as to whether well-known antioxidants can protect the cell or significantly reduce the effects of GO. This study focused on the possible remedial effect of vitamin C in Acheta domesticus intoxicated with GO for whole lives. The reproduction potential was measured at the level of Vitellogenin (Vg) gene expression, Vg protein expression, hatching success, and share of nutrition in the developing egg. There was no simple relationship between the Vg gene’s expression and the Vg protein content. Despite fewer eggs laid in the vitamin C groups, hatching success was high, and egg composition did not differ significantly. The exceptions were GO20 and GO20 + Vit. C groups, with a shift in the lipid content in the egg. Most likely, ascorbic acid impacts the level of Vg gene expression but does not affect the production of Vg protein or the quality of eggs laid. Low GO concentration in food did not cause adverse effects, but the relationship between GO toxicity and its concentration should be investigated more thoroughly. © 2024 by the authors.},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85201816769,

title = {Efficacy of propolis and royal jelly in attenuating cadmium-induced spermatogenesis and steroidogenesis dysregulation, causing infertility in rats},

author = { A. Amr and A.E.A. Karim and M. Augustyniak and A.E. Wakil and L.M. El-Samad and M.A. Hassan},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85201816769&doi=10.1007%2fs11356-024-34673-0&partnerID=40&md5=e7d746318c9f5313b17decf780654ebe},

doi = {10.1007/s11356-024-34673-0},

year  = {2024},

date = {2024-01-01},

journal = {Environmental Science and Pollution Research},

volume = {31},

number = {40},

pages = {53052-53073},

publisher = {Springer},

abstract = {Bee-derived pharmaceutical products, including propolis (PRO) and royal jelly (ROJ), possess outstanding pharmacological properties. However, their efficiency in counteracting the deleterious influences of cadmium (Cd) in testes and the relevant mechanisms entail further investigations. Therefore, this study sheds light on the therapeutic efficacy of PRO and ROJ against testicular dysfunction and infertility induced by Cd. Toward this end, 30 mature male Wistar albino rats were randomly divided into six groups (5 animals/group), including (I) control, (II) Cd, (III) PRO, (IV) ROJ, (V) PRO + Cd, and (VI) ROJ + Cd groups. Furthermore, antioxidant factors, semen quality, hormonal levels, steroidogenic enzymes, and genotoxicity were assessed. Moreover, histopathological and ultrastructural attributes and offspring rates were investigated. The Cd-treated group revealed marked reductions in reduced glutathione (GSH), total antioxidant capacity (TAC), and superoxide dismutase (SOD) with an amplification of lipid peroxidation in testes, indicating disruption of the antioxidant defense system. Furthermore, myeloperoxidase (MPO) activity and DNA damage were significantly heightened, implying inflammation and genotoxicity, respectively. Moreover, steroidogenic enzymes, including 17β-Hydroxy Steroid Dehydrogenase 3 (HSD17b3), 3β-Hydroxy Steroid Dehydrogenase 2 (HSD3b2), 17α-hydroxylase/17,20-lyase (CYP17A1), and steroid 5α-reductase 2 (SRD5A2) were markedly diminished accompanied with disorders in luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone. Besides, spermatozoa quality was reduced, associated with a diminution in the diameter of seminiferous tubules. By contrast, PRO or ROJ significantly protected and/or counteracted the Cd-induced pathophysiological consequences, ameliorating antioxidant and inflammatory biomarkers, steroidogenic enzymes, hormonal levels, and sperm properties, along with lessening DNA impairments. Critically, histological and ultrastructural analyses manifested several anomalies in the testicular tissues of the Cd-administered group, including the Leydig and Sertoli cells and spermatozoa. Conversely, PRO or ROJ sustained testicular tissues' structure, enhancing spermatozoa integrity and productivity. Interestingly, treatment with PRO or ROJ improved fertility indices through offspring rates compared to the Cd-animal group. Our data suggest that PRO is a more effective countermeasure than ROJ against Cd toxicity for securing the delicate testicular microenvironment for spermatogenesis and steroidogenesis. Graphical abstract: (Figure presented.) © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85187957924,

title = {Metallic nanoparticle actions on the outer layer structure and properties of Bacillus cereus and Staphylococcus epidermidis},

author = { O. Metryka and D. Wasilkowski and M. Dulski and M. Adamczyk-Habrajska and M. Augustyniak and A. Mrozik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85187957924&doi=10.1016%2fj.chemosphere.2024.141691&partnerID=40&md5=a9806854080a0feb4ece86cbf2198ab6},

doi = {10.1016/j.chemosphere.2024.141691},

year  = {2024},

date = {2024-01-01},

journal = {Chemosphere},

volume = {354},

publisher = {Elsevier Ltd},

abstract = {Although the antimicrobial activity of nanoparticles (NPs) penetrating inside the cell is widely recognised, the toxicity of large NPs (>10 nm) that cannot be translocated across bacterial membranes remains unclear. Therefore, this study was performed to elucidate the direct effects of Ag-NPs, Cu-NPs, ZnO-NPs and TiO2-NPs on relative membrane potential, permeability, hydrophobicity, structural changes within chemical compounds at the molecular level and the distribution of NPs on the surfaces of the bacteria Bacillus cereus and Staphylococcus epidermidis. Overall analysis of the results indicated the different impacts of individual NPs on the measured parameters in both strains depending on their type and concentration. B. cereus proved to be more resistant to the action of NPs than S. epidermidis. Generally, Cu-NPs showed the most substantial toxic effect on both strains; however, Ag-NPs exhibited negligible toxicity. All NPs had a strong affinity for cell surfaces and showed strain-dependent characteristic dispersion. ATR-FTIR analysis explained the distinctive interactions of NPs with bacterial functional groups, leading to macromolecular structural modifications. The results presented provide new and solid evidence for the current understanding of the interactions of metallic NPs with bacterial membranes. © 2024 Elsevier Ltd},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85178151628,

title = {Survival, growth and digestive functions after exposure to nanodiamonds - Transgenerational effects beyond contact time in house cricket strains},

author = { M. Augustyniak and A.K. Ajay and A. Kędziorski and M. Tarnawska and M.M. Rost-Roszkowska and B. Flasz and A. Babczyńska and B. Mazur and K. Rozpędek and R.S. Alian and M. Skowronek and E. Świerczek and Kl. Wiśniewska and P. Ziętara},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85178151628&doi=10.1016%2fj.chemosphere.2023.140809&partnerID=40&md5=8596e027ee47c37b61120de37d91f704},

doi = {10.1016/j.chemosphere.2023.140809},

year  = {2024},

date = {2024-01-01},

journal = {Chemosphere},

volume = {349},

publisher = {Elsevier Ltd},

abstract = {The long-term exposure effects of nanodiamonds (NDs), spanning an organism's entire lifespan and continuing for subsequent generation, remain understudied. Most research has focused on evaluating their biological impacts on cell lines and selected organisms, typically over short exposure durations lasting hours or days. The study aimed to assess growth, mortality, and digestive functions in wild (H) and long-lived (D) strains of Acheta domesticus (Insecta: Orthoptera) after two-generational exposure to NDs in concentrations of 0.2 or 2 mg kg−1 of food, followed by their elimination in the third generation. NDs induced subtle stimulating effect that depended on the strain and generation. In the first generation, more such responses occurred in the H than in the D strain. In the first generation of H strain insects, contact with NDs increased survival, stimulated the growth of young larvae, and the activity of most digestive enzymes in mature adults. The same doses and exposure time did not cause similar effects in the D strain. In the first generation of D strain insects, survival and growth were unaffected by NDs, whereas, in the second generation, significant stimulation of those parameters was visible. Selection towards longevity appears to support higher resistance of the insects to exposure to additional stressor, at least in the first generation. The cessation of ND exposure in the third generation caused potentially harmful changes, which included, e.g., decreased survival probability in H strain insects, slowed growth of both strains, as well as changes in heterochromatin density and distribution in nuclei of the gut cells in both strains. Such a reaction may suggest the involvement of epigenetic inheritance mechanisms, which may become inadequate after the stress factor is removed. © 2023 Elsevier Ltd},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85165896213,

title = {Reversal Effects of Royal Jelly and Propolis Against Cadmium-Induced Hepatorenal Toxicity in Rats},

author = { E.M. Omar and N.S. El-Sayed and F.Y. Elnozahy and E. Hassan and A. Amr and M. Augustyniak and L.M. El-Samad and A. El Wakil},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85165896213&doi=10.1007%2fs12011-023-03775-0&partnerID=40&md5=becbf46e52d61cff8ecafe838a7de5d3},

doi = {10.1007/s12011-023-03775-0},

year  = {2024},

date = {2024-01-01},

journal = {Biological Trace Element Research},

volume = {202},

number = {4},

pages = {1612-1627},

publisher = {Springer},

abstract = {Heavy metal toxicity is an exponentially growing health problem. In this study, we aimed to assess the protective properties of propolis and royal jelly against cadmium adverse effects. Thirty-two adult male rats were included in our study; kidney and liver functions, histopathological changes, and the level of oxidative stress were evaluated in rats exposed to a daily dose of 4.5 mg cadmium per kilogram of body weight for 1 month and those cotreated simultaneously with either propolis (50 mg/kg/day) or royal jelly (200 mg/kg/day) with cadmium compared to control animals. Cadmium-mediated hepatorenal toxicity was manifested as per the increased oxidative stress, function deterioration, and characteristic histopathological aberrations. The supplementation of royal jelly or propolis restores most of the affected parameters to a level similar to the control group. However, the parameters describing the grade of DNA damage and the interleukin-1β expression in the liver, as well as the levels of malondialdehyde and metallothionein, were slightly elevated compared to controls, despite the regular use of royal jelly or propolis. It is worth noting that better results were found in the case of royal jelly compared to propolis administration. Most likely, the ability of both products to chelate cadmium and contribute in reducing oxidative stress is of great importance. However, further investigations are needed to complement the knowledge about the expected nutritional and medicinal values. Graphical abstract: (Figure presented.). © The Author(s) 2023.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85185955844,

title = {Concentration- and Time-Dependent Dietary Exposure to Graphene Oxide and Silver Nanoparticles: Effects on Food Consumption and Assimilation, Digestive Enzyme Activities, and Body Mass in Acheta domesticus},

author = { R. Seyed Alian and B. Flasz and A. Kędziorski and Ł. Majchrzycki and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185955844&doi=10.3390%2finsects15020089&partnerID=40&md5=5297f84c7a1e136e6fa7655512ea890c},

doi = {10.3390/insects15020089},

year  = {2024},

date = {2024-01-01},

journal = {Insects},

volume = {15},

number = {2},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {The advancement of nanotechnology poses a real risk of insect exposure to nanoparticles (NPs) that can enter the digestive system through contaminated food or nanopesticides. This study examines whether the exposure of model insect species—Acheta domesticus—to increasing graphene oxide (GO) and silver nanoparticle (AgNP) concentrations (2; 20; and 200 ppm and 4; 40; and 400 ppm; respectively) could change its digestive functions: enzymes’ activities, food consumption, and assimilation. We noticed more pronounced alterations following exposure to AgNPs than to GO. They included increased activity of α-amylase, α-glucosidase, and lipase but inhibited protease activity. Prolonged exposure to higher concentrations of AgNPs resulted in a significantly decreased food consumption and changed assimilation compared with the control in adult crickets. A increase in body weight was observed in the insects from the Ag4 group and a decrease in body weight or no effects were observed in crickets from the Ag40 and Ag400 groups (i.e.; 4; 40; or 400 ppm of AgNPs; respectively), suggesting that even a moderate disturbance in nutrient and energy availability may affect the body weight of an organism and its overall condition. This study underscores the intricate interplay between NPs and digestive enzymes, emphasizing the need for further investigation to comprehend the underlying mechanisms and consequences of these interactions. © 2024 by the authors.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85178319468,

title = {In vitro and in silico evaluation of the antimicrobial and antioxidant activities of spiropyrazoline oxindole congeners},

author = { M. Chalkha and K. Chebbac and H. Nour and A. Nakkabi and A. El Moussaoui and B. Tüzün and M. Bourhia and S. Chtita and M. Bakhouch and H. Laaroussi and S.M. Kawsar and T.B. Hadda and G. Al Houari and M. Augustyniak and M.A.M. Aboul-Soud and M. El Yazidi},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85178319468&doi=10.1016%2fj.arabjc.2023.105465&partnerID=40&md5=4d20b32617e0af842c2e46bd7d062e4b},

doi = {10.1016/j.arabjc.2023.105465},

year  = {2024},

date = {2024-01-01},

journal = {Arabian Journal of Chemistry},

volume = {17},

number = {1},

publisher = {Elsevier B.V.},

abstract = {The search for novel powerful antimicrobial and antioxidant agents is considered a dynamic field in medicinal chemistry. In this context, a series of spiropyrazoline indolin-3-one congeners were assessed for their in vitro bioactivities, and in-silico studies were conducted to support the experimental results. The antimicrobial screening of the spiropyrazoline oxindole congeners against the selected microbe strains (Staphylococcus aureus (CECT 976); Bacillus subtilis (DSM 6633); Escherichia coli (K12); and Candida albicans (ATCC 10231)) exhibited moderate to excellent, compared to control standard antibiotics (Ampicillin; streptomycin and fluconazole). This activity was observed to be tightly dependent upon the nature of the substituents carried by the aromatic rings. Moreover, the tested compounds showed variable dose-dependent antioxidant activity. Notably, congeners 2c, 2d and 2e exhibited a remarkable antioxidant activity, due to the positive impact of the electron-donating groups (CH3 and OCH3) on the antioxidant activity. Density functional theory (DFT) simulations were executed on the target molecules to better understand their structural and electronic properties, as well as to explain the results obtained from the antioxidant activity. The molecular docking studies showed that the studied congeners have good binding affinities and interactions with the target proteins (catalase compound II and CYP51). Moreover, the 100 ns molecular dynamics (MD) simulation analysis was conducted to follow the behavior of the complexes formed between ligand 2e and the target proteins (2CAG and 5V5Z) under in-silico physiological conditions to explore and evaluate its stability over time. MD simulation indicated a stable conformation and binding patterns in a stimulating environment of the congeners (2CAG-2e and 5V5Z-2e). The results of Petra/Osiris/Molinspiration (POM) analyses suggested that all the spiranic cycloadducts have good oral bioavailability and pharmacokinetics without any evidence of observed toxicity. Taken together, our findings provide valuable experimental and theoretical information that will be helpful for designing novel spiranic molecules with potential pharmacological applications. © 2023},

note = {13},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85183424176,

title = {Does Selection for Longevity in Acheta domesticus Involve Sirtuin Activity Modulation and Differential Response to Activators (Resveratrol and Nanodiamonds)?},

author = { P. Ziętara and B. Flasz and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85183424176&doi=10.3390%2fijms25021329&partnerID=40&md5=1c2a1a8663d243d4f29ace7d245bfacf},

doi = {10.3390/ijms25021329},

year  = {2024},

date = {2024-01-01},

journal = {International Journal of Molecular Sciences},

volume = {25},

number = {2},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {Sirtuins, often called “longevity enzymes”, are pivotal in genome protection and DNA repair processes, offering insights into aging and longevity. This study delves into the potential impact of resveratrol (RV) and nanodiamonds (NDs) on sirtuin activity, focusing on two strains of house crickets (Acheta domesticus): the wild-type and long-lived strains. The general sirtuin activity was measured using colorimetric assays, while fluorescence assays assessed SIRT1 activity. Additionally, a DNA damage test and a Kaplan–Meier survival analysis were carried out. Experimental groups were fed diets containing either NDs or RV. Notably, the long-lived strain exhibited significantly higher sirtuin activity compared to the wild-type strain. Interestingly, this heightened sirtuin activity persisted even after exposure to RVs and NDs. These findings indicate that RV and NDs can potentially enhance sirtuin activity in house crickets, with a notable impact on the long-lived strain. This research sheds light on the intriguing potential of RV and NDs as sirtuin activators in house crickets. It might be a milestone for future investigations into sirtuin activity and its potential implications for longevity within the same species, laying the groundwork for broader applications in aging and lifespan extension research. © 2024 by the authors.},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85180363492,

title = {Adaptation by death? Cell death-based tolerance to cadmium in 150-generation exposure of Spodoptera exiqua Hübner (Lepidoptera: Noctuidae)},

author = { A. Babczyńska and M.M. Rost-Roszkowska and A. Kafel and B. Łozowski and M. Augustyniak and M. Tarnawska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85180363492&doi=10.1093%2fee%2fnvad077&partnerID=40&md5=65b08164a16d96d7682214b4d1536977},

doi = {10.1093/ee/nvad077},

issn = {0046225X},

year  = {2023},

date = {2023-01-01},

journal = {Environmental Entomology},

volume = {52},

number = {6},

pages = {1057-1070},

publisher = {Entomological Society of America},

abstract = {Mechanisms, including autophagy and apoptosis, which serve to regulate and ensure proper organism functions under optimal conditions, play additional defensive roles under environmental pressure.The aim of this study was to test the following hypotheses: (i) elevated autophagy and apoptosis intensity levels, as defensive processes in response to contact with cadmium, are maintained for a limited number of generations and (ii) the number of generations after which levels of cell death processes reach the reference level depends on selective pressure. Cell death processes were assessed by light and transmission electron microscopy, terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL), and cytometric analyses. Model insects (Spodoptera exiqua; Hübner; 1808) were orally exposed to various concentrations of cadmium for 18 generations and compared with reference strains exposed to cadmium or not (control) for over 150 generations. Elevated programmed cell death intensity levels decreased after several generations, indicating tolerance of individuals to cadmium in the diet and verifying the first hypothesis; however, testing the second hypothesis indicated that the number of generations depended not only on pressure intensity, but also on cell death type, since levels of autophagy remained increased for a minimum of 12 generations. © The Author(s) 2023. Published by Oxford University Press on behalf of Entomological Society of America.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85175249684,

title = {Sericin and melatonin mitigate diethylnitrosamine-instigated testicular impairment in mice: Implications of oxidative stress, spermatogenesis, steroidogenesis, and modulation of Nrf2/WT1/SF-1 signaling pathways},

author = { E.S. Habiba and S.A. Harby and N.S. El-Sayed and E.M. Omar and B.A. Bakr and M. Augustyniak and L.M. El-Samad and M.A. Hassan},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85175249684&doi=10.1016%2fj.lfs.2023.122220&partnerID=40&md5=2a70f26de284dd465625ff3d211dd6d4},

doi = {10.1016/j.lfs.2023.122220},

issn = {00243205},

year  = {2023},

date = {2023-01-01},

journal = {Life Sciences},

volume = {334},

publisher = {Elsevier Inc.},

abstract = {Aims: This study aimed to investigate the therapeutic influence of combination therapy with sericin and melatonin on attenuating diethylnitrosamine (DEN)-instigated testicular dysfunction in mice and defining the molecular mechanisms involved in orchestrating redox signaling pathways and restoring spermatogenesis and steroidogenesis. Materials and methods: Different groups of male Swiss albino mice were established and injected with respective drugs intraperitoneally. Semen analysis, hormonal assays, and oxidative stress biomarkers were evaluated. Additionally, melatonin and its receptors, WT1, SF-1, vimentin, Nrf2, and ANXA1 expressions were assessed. Histopathological and ultrastructural features of the testes were investigated by semithin, SEM, and TEM analyses. Key findings: Exposure to DEN exhibited pathophysiological consequences, including a remarkable increase in lipid peroxidation associated with substantial diminutions in SOD, CAT, GPx, GSH, GSH:GSSG, and GST. Furthermore, it disrupted spermatozoa integrity, testosterone, FSH, LH, melatonin, and its receptors (MT1 and MT2) levels, implying spermatogenesis dysfunction. By contrast, treatment with sericin and melatonin significantly restored these disturbances. Interestingly, the combination therapy of sericin and melatonin noticeably augmented the Nrf2, WT1, and SF-1 expressions compared to DEN-treated mice, deciphering the amelioration perceived in antioxidant defense and spermatogenesis inside cells. Furthermore, immunohistochemical detection of ANXA1 alongside histopathological and ultrastructural analyses revealed evident maintenance of testicular structures without discernible inflammation or anomalies in mice administered with sericin and melatonin compared to the DEN-treated group. Significance: Our findings highlighted that treatment with sericin and melatonin alleviated the testicular tissues in mice from oxidative stress and dysregulated spermatogenesis and steroidogenesis engendered by DEN. © 2023 Elsevier Inc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85171778833,

title = {Assessing the Risk of Groundwater Pollution in Northern Algeria through the Evaluation of Influencing Parameters and Ensemble Methods},

author = { S. Tachi and H. Bouguerra and M. Djellal and O. Benaroussi and A. Belaroui and B. Łozowski and M. Augustyniak and S. Benmamar and S. Benziada and A. Woźnica},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85171778833&doi=10.1134%2fS1028334X23600767&partnerID=40&md5=5fba9f721fd68500590b1bb141ebd127},

doi = {10.1134/S1028334X23600767},

issn = {1028334X},

year  = {2023},

date = {2023-01-01},

journal = {Doklady Earth Sciences},

volume = {513},

number = {1},

pages = {1233-1243},

publisher = {Pleiades Publishing},

abstract = {Abstract: Developing an accurate map to control groundwater pollution is becoming increasingly important to fulfill requirements of the Algerian water directives. Evaluating groundwater vulnerability to nitrate contamination in the eastern Mitidja aquifer has become crucial for water resource control and preservation. In this research, some of the commonly used ensemble methods namely Categorical Boosting, Adaptive Boosting and Random Forest were employed to model the spatial groundwater vulnerability to nitrate concentration based on the maximum acceptable concentration in drinking water according to the Algerian directives (50 mg/L). The study were carried out on the evaluation of different possible influencing parameters including depth to groundwater, permeability, rainfall, vadose zone, distance to river, drainage density, land use land cover, Normalized Difference Vegetation Index, slope, topographic wetness index and elevation. The proposed approach aims to understand and control the vulnerability and pollution process of the groundwater aquifers with the smallest available dataset to assess vulnerability predictive mapping. The best results based on the receiver operating characteristic and area under the curve (ROC-AUC) showed that the Categorical boosting indicated the highest accuracy of 94% then the adaptive boosting algorithm with 92%, and the Random forest algorithm with 89%. It revealed that climatic and hydrogeological factors are the key factors determining groundwater vulnerability to NO3 concentration in the eastern Mitidja. It is concluded that the risk of groundwater pollution covers a large area of the groundwater resources in the city of Algiers, Blida, and Boumerdas in northern Algeria. © 2023, Pleiades Publishing, Ltd.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85167582630,

title = {A comprehensive review of recent advances in silk sericin: Extraction approaches, structure, biochemical characterization, and biomedical applications},

author = { M. Saad and L.M. El-Samad and R.A. Gomaa and M. Augustyniak and M.A. Hassan},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85167582630&doi=10.1016%2fj.ijbiomac.2023.126067&partnerID=40&md5=e1b1547f308869f6496858d1f21adb0a},

doi = {10.1016/j.ijbiomac.2023.126067},

issn = {01418130},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Biological Macromolecules},

volume = {250},

publisher = {Elsevier B.V.},

abstract = {Silks are natural polymers that have been widely used for centuries. Silk consists of a filament core protein, termed fibroin, and a glue-like coating substance formed of sericin (SER) proteins. This protein is extracted from the silkworm cocoons (particularly Bombyx mori) and is mainly composed of amino acids like glycine, serine, aspartic acid, and threonine. Silk SER can be obtained using numerous methods, including enzymatic extraction, high-temperature, autoclaving, ethanol precipitation, cross-linking, and utilizing acidic, alkali, or neutral aqueous solutions. Given the versatility and outstanding properties of SER, it is widely fabricated to produce sponges, films, and hydrogels for further use in diverse biomedical applications. Hence, many authors reported that SER benefits cell proliferation, tissue engineering, and skin tissue restoration thanks to its moisturizing features, antioxidant and anti-inflammatory properties, and mitogenic effect on mammalian cells. Remarkably, SER is used in drug delivery depending on its chemical reactivity and pH-responsiveness. These unique features of SER enhance the bioactivity of drugs, facilitating the fabrication of biomedical materials at nano- and microscales, hydrogels, and conjugated molecules. This review thoroughly outlines the extraction techniques, biological properties, and respective biomedical applications of SER. © 2023 Elsevier B.V.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85169134510,

title = {Multigenerational Effects of Graphene Oxide Nanoparticles on Acheta domesticus DNA Stability},

author = { B. Flasz and A.K. Ajay and M. Tarnawska and A. Babczyńska and Ł. Majchrzycki and A. Kędziorski and Ł. Napora-Rutkowski and E. Świerczek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85169134510&doi=10.3390%2fijms241612826&partnerID=40&md5=94952f38d3a2995d15e764932e1dfb79},

doi = {10.3390/ijms241612826},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {16},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {The use of nanoparticles like graphene oxide (GO) in nanocomposite industries is growing very fast. There is a strong concern that GO can enter the environment and become nanopollutatnt. Environmental pollutants’ exposure usually relates to low concentrations but may last for a long time and impact following generations. Attention should be paid to the effects of nanoparticles, especially on the DNA stability passed on to the offspring. We investigated the multigenerational effects on two strains (wild and long-lived) of house cricket intoxicated with low GO concentrations over five generations, followed by one recovery generation. Our investigation focused on oxidative stress parameters, specifically AP sites (apurinic/apyrimidinic sites) and 8-OHdG (8-hydroxy-2′-deoxyguanosine), and examined the global DNA methylation pattern. Five intoxicated generations were able to overcome the oxidative stress, showing that relatively low doses of GO have a moderate effect on the house cricket (8-OHdG and AP sites). The last recovery generation that experienced a transition from contaminated to uncontaminated food presented greater DNA damage. The pattern of DNA methylation was comparable in every generation, suggesting that other epigenetic mechanisms might be involved. © 2023 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85164355155,

title = {Joint cadmium and polypropylene microparticle action in cadmium tolerant model insect},

author = { A. Babczyńska and Mi. Górka and A. Lis and M. Tarnawska and B. Łozowski and J. Brożek and K. Rozpędek and M. Augustyniak and M. Skowronek and A. Kafel},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85164355155&doi=10.1016%2fj.etap.2023.104209&partnerID=40&md5=fd1f5028f306c019e8d9e70db430f033},

doi = {10.1016/j.etap.2023.104209},

issn = {13826689},

year  = {2023},

date = {2023-01-01},

journal = {Environmental Toxicology and Pharmacology},

volume = {101},

publisher = {Elsevier B.V.},

abstract = {Microplastic enlisted as a contaminant of emerging concerns in polluted environments interact with “traditional” contaminants such as metals, causing, among others, their increased accumulation in the body. Harmful effects depend on the exposed animals' possible preadaptation and/or cross-tolerance. The project aimed to assess the role of this phenomenon in the limited toxicity of polypropylene fibers (PPf) in 0%, 0.02%, 0.06, 0.18%, 0.54%, and 1.6% of Cd-supplemented food of larvae of Spodoptera exigua multigenerationally selected to cadmium tolerance. The activity of 20 digestive enzymes (API-ZYM test), defensins, and heat shock proteins, HSP70 levels in the exposed groups were used as biomarkers. PPfs caused the increase of Cd accumulation in the body, while intake of polypropylene microfibers did not change the biomarker levels. Moreover, multigenerational Cd pre-exposure, due to increased tolerance of Cd and, possibly, cross-tolerance, prepares the insects for an additional stressor (PPf) alone and in interaction with cadmium. © 2023 Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85152414271,

title = {Native Bacteria Isolated from Phosphate Deposits Reveal Efficient Metal Biosorption and Adhesion to Ore Particles},

author = { H. Rabia and M. Ould Hamou and K. Kasperkiewicz and T. Krzykawski and M. Malicka and I.W. Potocka and I. Bodnaruk and A. Merchichi and M. Skowronek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85152414271&doi=10.3390%2fmin13030388&partnerID=40&md5=1bfaf6d1a995b0e8847e053403d2f345},

doi = {10.3390/min13030388},

issn = {2075163X},

year  = {2023},

date = {2023-01-01},

journal = {Minerals},

volume = {13},

number = {3},

publisher = {MDPI},

abstract = {Mining and processing phosphate ore are among the essential branches of the economy in some developing countries, including Algeria. Conventional ore beneficiation methods can harm the environment by consuming tremendous amounts of water resources (during washing and flotation), potentially hazardous chemicals, and thermal energy. Mine water contains toxic metals that, when released, interfere with environmental functioning. Therefore, in line with environmental needs, conventional methods should be gradually replaced with safe biotechnological processes. This study aimed to investigate the biosorption and adhesion abilities of native microorganisms isolated from Djebel Onk ore (Algeria). The examined bacterial strains differed in their metal accumulation efficiency. The incubation of phosphate ore with the native strain Bacillus HK4 significantly increased the recovery of Mg and Cd (at pH 7; 8147.00 and 100.89 µg/g−1; respectively). The HK4 strain also revealed better adhesion to the ore particles than the reference strain of Bacillus subtilis. Thus, biosorption could be more effective when using the native HK4 strain, which can remove Cd and/or Mg over a pH 4–10 range. Moreover, concerning the unique adhesion capacity of HK4, the strain can be considered in the design of bioflotation methods, as well as in the development of an eco-friendly method of ore and post-flotation waste beneficiation. © 2023 by the authors.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85145976280,

title = {Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus},

author = { B. Flasz and M. Dziewięcka and A.K. Ajay and M. Tarnawska and A. Babczyńska and A. Kędziorski and Ł. Napora-Rutkowski and P. Ziętara and E. Świerczek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145976280&doi=10.3390%2fijms24010290&partnerID=40&md5=5d497b4e9e8b4082341d87d5be39d3d6},

doi = {10.3390/ijms24010290},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {1},

publisher = {MDPI},

abstract = {The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage; double-strand breaks—DSB; 8-hydroxy-2′-deoxyguanosine-8-OHdG; abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms. © 2022 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85145973153,

title = {Why Is Longevity Still a Scientific Mystery? Sirtuins—Past, Present and Future},

author = { P. Ziętara and M. Dziewięcka and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145973153&doi=10.3390%2fijms24010728&partnerID=40&md5=74cffafd587a03ac6e53845926815874},

doi = {10.3390/ijms24010728},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {1},

publisher = {MDPI},

abstract = {The sirtuin system consists of seven highly conserved regulatory enzymes responsible for metabolism, antioxidant protection, and cell cycle regulation. The great interest in sirtuins is associated with the potential impact on life extension. This article summarizes the latest research on the activity of sirtuins and their role in the aging process. The effects of compounds that modulate the activity of sirtuins were discussed, and in numerous studies, their effectiveness was demonstrated. Attention was paid to the role of a caloric restriction and the risks associated with the influence of careless sirtuin modulation on the organism. It has been shown that low modulators’ bioavailability/retention time is a crucial problem for optimal regulation of the studied pathways. Therefore, a detailed understanding of the modulator structure and potential reactivity with sirtuins in silico studies should precede in vitro and in vivo experiments. The latest achievements in nanobiotechnology make it possible to create promising molecules, but many of them remain in the sphere of plans and concepts. It seems that solving the mystery of longevity will have to wait for new scientific discoveries. © 2022 by the authors.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85145920017,

title = {UPLC-MS/MS Analysis of Naturally Derived Apis mellifera Products and Their Promising Effects against Cadmium-Induced Adverse Effects in Female Rats},

author = { A. Amr and A.A. Abd el-Wahed and H.R. El-Seedi and S.A.M. Khalifa and M. Augustyniak and L.M. El-Samad and A.E. Abdel-Karim and A. El Wakil},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145920017&doi=10.3390%2fnu15010119&partnerID=40&md5=546f577b73e63eeed86b21d8ee63f7d1},

doi = {10.3390/nu15010119},

issn = {20726643},

year  = {2023},

date = {2023-01-01},

journal = {Nutrients},

volume = {15},

number = {1},

publisher = {MDPI},

abstract = {Honeybee products arouse interest in society due to their natural origin and range of important biological properties. Propolis (P) and royal jelly (RJ) attract scientists’ attention because they exhibit antioxidant, anti-inflammatory, anti-bacterial, anti-tumor, and immunomodulatory abilities. In this study, we tested whether P and RJ could mitigate the adverse effects of cadmium (Cd) exposure, with particular emphasis on the reproductive function in female rats. In this line, one week of pretreatment was established. Six experimental groups were created, including (i) the control group (without any supplementation), (ii) the Cd group (receiving CdCl2 in a dose of 4.5 mg/kg/day), (iii) the P group (50 mg of P/kg/day), (iv) RJ group (200 mg of RJ/kg/day), (v) P + Cd group (rats pretreated with P and then treated with P and Cd simultaneously), (vi) RJ + Cd group (animals pretreated with RJ before receiving CdCl2 simultaneously with RJ). Cd treatment of rats adversely affected a number of measured parameters, including body weight, ovarian structure and ultrastructure, oxidative stress parameters, increased ovarian Cd content and prolonged the estrous cycle. Pretreatment and then cotreatment with P or RJ and Cd alleviated the adverse effects of Cd, transferring the clusters in the PCA analysis chart toward the control group. However, clusters for cotreated groups were still distinctly separated from the control and P, or RJ alone treated groups. Most likely, investigated honeybee products can alter Cd absorption in the gut and/or increase its excretion through the kidneys and/or mitigate oxidative stress by various components. Undoubtedly, pretreatment with P or RJ can effectively prepare the organism to overcome harmful insults. Although the chemical composition of RJ and P is relatively well known, focusing on proportion, duration, and scheme of treatment, as well as the effects of particular components, may provide interesting data in the future. In the era of returning to natural products, both P and RJ seem valuable materials for further consideration as anti-infertility agents. © 2022 by the authors.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85139403610,

title = {Hepatoprotective effects of a chemically-characterized extract from artichoke (Cynara scolymus L.) against AFB1-induced toxicity in rats},

author = { M.A. Nasef and M.I. Yousef and D.A. Ghareeb and M. Augustyniak and M.A.M. Aboul-Soud and A. El Wakil},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85139403610&doi=10.1080%2f01480545.2022.2129672&partnerID=40&md5=17ab4939d678a4967a3855ad0f067b0e},

doi = {10.1080/01480545.2022.2129672},

issn = {01480545},

year  = {2023},

date = {2023-01-01},

journal = {Drug and Chemical Toxicology},

volume = {46},

number = {6},

pages = {1070-1082},

publisher = {Taylor and Francis Ltd.},

abstract = {This study was conducted to investigate the protective potential of a pharmaceutically formulated capsule of artichoke leaf powder (ArLP) against aflatoxin B1 (AFB1)-induced hepatotoxicity in male albino rats. In the 42-day experiment, rats were divided into five equal groups: (i) control, treated with sterile water, (ii) treated with 4% DMSO as AFB1 vehicle, (iii) ArLP of 100 mg kg–1 bw, (iv) AFB1 of 72 µg kg–1 bw, and (v) AFB1 plus ArLP. Exposure of rats to AFB1 resulted in hepatotoxicity as manifested by the intensification of oxidative stress, production of free radicals and significant increase in the activity levels of liver function enzymes relative to the control. Significant reductions in both the enzymatic and non-enzymatic antioxidant markers as well as histopathological abnormalities in liver tissues were also observed. Notably, the combined administration of ArLP with AFB1 clearly reduced AFB1-mediated adverse effects leading to the normalization of most of these parameters back to control levels. These findings clearly highlight the potential benefits of artichoke dietary supplements as a safe and natural solution in counteracting the adverse hepatotoxic effects conferred by AFB1 exposure. Further research is warranted to fully dissect the biochemical and molecular mechanism of action of the observed artichoke-mediated hepatoprotection. © 2022 Informa UK Limited, trading as Taylor & Francis Group.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85143899745,

title = {Synthesis, Characterization, DFT Mechanistic Study, Antimicrobial Activity, Molecular Modeling, and ADMET Properties of Novel Pyrazole-isoxazoline Hybrids},

author = { M. Chalkha and H. Nour and K. Chebbac and A. Nakkabi and L. Bahsis and M. Bakhouch and M. Akhazzane and M. Bourass and S. Chtita and Y.A. Bin Jardan and M. Augustyniak and M. Bourhia and M.A.M. Aboul-Soud and M. El Yazidi},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85143899745&doi=10.1021%2facsomega.2c05788&partnerID=40&md5=93de20a6a2c14df4feee7c3aaa6eb30c},

doi = {10.1021/acsomega.2c05788},

issn = {24701343},

year  = {2022},

date = {2022-01-01},

journal = {ACS Omega},

volume = {7},

number = {50},

pages = {46731-46744},

publisher = {American Chemical Society},

abstract = {A series of new heterocycle hybrids incorporating pyrazole and isoxazoline rings was successfully synthesized, characterized, and evaluated for their antimicrobial responses. The synthesized compounds were obtained utilizing N-alkylation and 1,3-dipolar cycloaddition reactions, as well as their structures were established through spectroscopic methods and confirmed by mass spectrometry. To get more light on the regioselective synthesis of new hybrid compounds, mechanistic studies were performed using DFT calculations with B3LYP/6-31G(d;p) basis set. Additionally, the results of the preliminary screening indicate that some of the examined hybrids showed potent antimicrobial activity, compared to standard drugs. The results confirm that the antimicrobial activity is strongly dependent on the nature of the substituents linked pyrazole and isoxazoline rings. Furthermore, molecular docking studies were conducted to highlight the interaction modes between the investigated hybrid compounds and the Escherichia coli and Candida albicans receptors. Notably, the results demonstrate that the investigated compounds have strong protein binding affinities. The stability of the formed complexes by the binding between the hybrid compound 6c, and the target proteins was also confirmed using a 100 ns molecular dynamics simulation. Finally, the prediction of ADMET properties suggests that almost all hybrid compounds possess good pharmacokinetic profiles and no signs of observed toxicity, except for compounds 6e, 6f, and 6g. © 2022 American Chemical Society.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85141479039,

title = {Carboxymethyl cellulose/sericin-based hydrogels with intrinsic antibacterial, antioxidant, and anti-inflammatory properties promote re-epithelization of diabetic wounds in rats},

author = { L.M. El-Samad and M.A. Hassan and A.A. Basha and S. El-Ashram and E.H. Radwan and K.K. Abdul-Aziz and T.M. Tame and M. Augustyniak and A. El Wakil},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85141479039&doi=10.1016%2fj.ijpharm.2022.122328&partnerID=40&md5=c48c116e1cf0a358baca2e12f2199f00},

doi = {10.1016/j.ijpharm.2022.122328},

issn = {03785173},

year  = {2022},

date = {2022-01-01},

journal = {International Journal of Pharmaceutics},

volume = {629},

publisher = {Elsevier B.V.},

abstract = {Diabetic wound healing encounters significant challenges due to the extreme oxidative stress resulting from excessive inflammation and microbial infections, disrupting the typical cascade of wound healing and thwarting the re-epithelialization of skin tissues. Benefiting from the biological activities of carboxymethyl cellulose (CMC) and sericin, we thus fabricated multifunctional hydrogels of CMC-Sericin. The hydrogel revealed high swelling performance alongside its porous structure. The incorporation of sericin bestowed the CMC-Sericin hydrogels with a prominent capacity to scavenge free radicals and antibacterial activity. In vivo investigations using diabetic full-thickness excision wounds demonstrated the capability of CMC-Sericin dressing to enhance diabetic wounds in rats treated or untreated with insulin concurrently. Furthermore, histopathological examinations manifested the skin tissue regeneration evidenced by the development of skin appendages like hair follicles and collagen deposition after treatment with CMC-Sericin hydrogel. Moreover, the levels of antioxidant parameters, including GSH and SOD, were substantially augmented and associated with a significant diminution in lipid peroxidation, implying a decrease in oxidative stress in the tissues. Beyond that, CMC-Sericin dressing downregulated the pro-inflammatory markers and upregulated the heat shock proteins, indicating the restoration of physiological features in cells. Strikingly, CMC-Sericin dressing remarkably promoted the healing of diabetic wounds without insulin treatment. © 2022 Elsevier B.V.},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85142191865,

title = {Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice},

author = { E. Hassan and S. Magdy and A. Attaallah and E. Gaber and O. Mansour and R.A. Gomaa and H. Odessy and M. Augustyniak and L.M. El-Samad and A. El Wakil},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85142191865&doi=10.1186%2fs12958-022-01032-y&partnerID=40&md5=08cfbd6cf509d93bab17a3684a8d5873},

doi = {10.1186/s12958-022-01032-y},

issn = {14777827},

year  = {2022},

date = {2022-01-01},

journal = {Reproductive Biology and Endocrinology},

volume = {20},

number = {1},

publisher = {BioMed Central Ltd},

abstract = {Background: Steroidogenesis is a complex process of sequential enzymatic reactions affected by climate change. Animals respond to altered day length, the so-called photoperiod, with changes in physiology. The study aimed to an evaluation of sericin effect in alleviating steroidogenesis disorders induced by disturbed photoperiod in mice. Methods: The animals were randomly divided into three groups according to the lighting cycle: a control group with a standard 12Light:12Dark cycle, a short-term photoperiod group with a 6Light:18Dark cycle, and a long-term photoperiod group with an 18Light:6Dark cycle. Both short and long-term groups were subdivided into two equal subgroups: The placebo and the sericin-treated subgroups received, for five weeks from prepubertal throughout adulthood, one intraperitoneal injection per week of the solvent and 1 g sericin/kg body weight, respectively. Results: Selected oxidative stress parameters and testicular and adrenal steroidogenic capacities of adult mice were measured. After five weeks, the placebo group with impaired photoperiod showed a decrease in the quality and quantity of sperm and a reduction in testosterone, corticosterone, aldosterone, total antioxidant capacity, xanthine oxidase, and melatonin. At the same time, in these groups, there was an increase in the level of aromatase, malondialdehyde, cholesterol, and steroidogenic factor-1 (SF-1) expression in the adrenal cortex and an enhancement in histological lesions. Mice receiving sericin had parameters similar to the control group. Conclusion: Our findings reveal that silk sericin can reduce the stress caused by photoperiod disorders regarding testicular function, sex hormone levels, and sperm quantity and quality. Thus, sericin is a biocompatible protein with a promising potential for its use in the case of organisms living under an abnormal photoperiod. © 2022, The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85140340059,

title = {CuO NPs-induced damage to testes and deregulation of the antioxidant system in wild terrestrial organism Blaps sulcata (Coleoptera: Tenebrionidae)},

author = { L.M. El-Samad and A.H. El-Gendy and A.M. Abdel-Moneim and S. El-Ashram and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140340059&doi=10.1016%2fj.enmm.2022.100751&partnerID=40&md5=8ee9bc14bdcf990a12c40bbab5c05c45},

doi = {10.1016/j.enmm.2022.100751},

issn = {22151532},

year  = {2022},

date = {2022-01-01},

journal = {Environmental Nanotechnology, Monitoring and Management},

volume = {18},

publisher = {Elsevier B.V.},

abstract = {Considering the constantly growing areas of CuO NPs applications, including agriculture, horticulture, and forestry, one can suspect that the balance in soil ecosystems can be seriously disturbed. Such a scenario causes serious concern about the fate of wild animals inhabiting these ecosystems. The study aimed to estimate the effects of a single exposure of Blaps sulcata, a valuable indicator of terrestrial environments pollution, on copper oxide nanoparticles (CuO NPs), emphasizing changes in the gonads. Survival curves were estimated, and LD50 was calculated as 0.0352 mg ∙ g-1b.w. Moreover, 30 days after the CuO NPs injection, an increased accumulation of Cu in the testes and numerous degenerative changes in this organ was found. These changes were accompanied by an intensification of apoptosis, DNA damage, and dysregulation of the antioxidant system, which was manifested by an increase in the activity of superoxide dismutase (SOD) with a simultaneous decrease in the activity of catalase (CAT). Reduced acetylcholinesterase (AChE) activity was also observed, suggesting that the exposed insects may have problems with neurotransmission and bear negative behavioral consequences - from obtaining food and avoiding predators to mating and reproductive behavior. The far-reaching effects of exposure of wild animals, including terrestrial insects, to CuO NPs, may be reproductive abnormalities and disturbances in populations and ecosystems. Thus, using CuO NPs, including fertilizers, growth stimulators, and pesticides, must be carefully considered in the future. © 2022 The Author(s)},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85130316325,

title = {Reduced body length and morphological disorders in Chrysomya albiceps (Diptera: Calliphoridae) larvae reared on aluminum phosphide-treated rabbits},

author = { S. El-Ashram and N.A. Toto and A. El Wakil and M. Augustyniak and L.M. El-Samad},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130316325&doi=10.1038%2fs41598-022-12351-w&partnerID=40&md5=d20babd6706ecb0eb653c362d5dbfe84},

doi = {10.1038/s41598-022-12351-w},

issn = {20452322},

year  = {2022},

date = {2022-01-01},

journal = {Scientific Reports},

volume = {12},

number = {1},

publisher = {Nature Research},

abstract = {Assessing the time of death based on the growth and development of insects is a critical task in forensic entomology. The rate of larvae development can be affected by a variety of toxins, including pesticides. Aluminum phosphide (AlP) is a low-cost insecticide that has yet to be tested for entomotoxicological significance, despite the fact that it is frequently the cause of fatal poisoning. In this study, we measured the body length of Chrysomya albiceps larvae reared on the carcasses of rabbits poisoned with AlP and analyzed the morphological changes of the larvae reared on the carcasses of rabbits poisoned with AlP. The concentration of AlP in the body of the larvae was significantly lower than in rabbit tissues. Insects from the AlP group had a significantly lower gain in body length. Furthermore, deformities in the larvae were found. Smaller respiratory spiracles were found, as well as a deformed small posterior end with hypogenesis of the posterior respiratory spiracles. Thus, disturbed growth and development of carrion flies found at a crime scene could indicate pesticide poisoning, such as aluminum phosphide. © 2022, The Author(s).},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85131527033,

title = {Does age pay off? Effects of three-generational experiments of nanodiamond exposure and withdrawal in wild and longevity-selected model animals},

author = { M. Augustyniak and A. Babczyńska and M. Dziewięcka and B. Flasz and J. Karpeta-Kaczmarek and A. Kędziorski and B. Mazur and K. Rozpędek and R. Seyed Alian and M. Skowronek and E. Świerczek and A. Świętek and M. Tarnawska and Kl. Wiśniewska and P. Ziętara},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131527033&doi=10.1016%2fj.chemosphere.2022.135129&partnerID=40&md5=a9ef4d24a7c7b03b85af2b6ebf7bc9e4},

doi = {10.1016/j.chemosphere.2022.135129},

issn = {00456535},

year  = {2022},

date = {2022-01-01},

journal = {Chemosphere},

volume = {303},

publisher = {Elsevier Ltd},

abstract = {Nanodiamonds (NDs) are considered a material with low toxicity. However, no studies describe the effects of ND withdrawal after multigenerational exposure. The aim was to evaluate ND exposure (in the 1st and 2nd generations) effects at low concentrations (0.2 or 2 mg kg−1) and withdrawal (in the 3rd generation) in the wild (H) and longevity-selected (D) model insect Acheta domesticus. We measured selected oxidative stress parameters, immunity, types of cell death, and DNA damage. Most of the results obtained in the 1st generation, e.g., catalase (CAT), total antioxidant capacity (TAC), heat shock proteins (HSP70), defensins, or apoptosis level, confirmed no significant toxicity of low doses of NDs. Interestingly, strain-specific differences were observed. D-strain crickets reduced autophagy, the number of ROS+ cells, and DNA damage. The effect can be a symptom of mobilization of the organism and stimulation of physiological defense mechanisms in long-living organisms. The 2nd-generation D-strain insects fed ND-spiked food at higher concentrations manifested a reduction in CAT, TAC, early apoptosis, and DNA damage, together with an increase in HSP70 and defensins. ROS+ cells and cells with reduced membrane potential and autophagy did not differ significantly from the control. H-strain insects revealed a higher number of ROS+ cells and cells with reduced membrane potential, decreased CAT activity, and early apoptosis. Elimination of NDs from the diet in the 3rd generation did not cause full recovery of the measured parameters. We noticed an increase in the concentration of HSP70 and defensins (H-strain) and a decrease in apoptosis (D-strain). However, the most visible increase was a significant increase in DNA damage, especially in H-strain individuals. The results suggest prolonged adverse effects of NDs on cellular functions, reaching beyond “contact time” with these particles. Unintentional and/or uncontrolled ND pollution of the environment poses a new challenge for all organisms inhabiting it, particularly during multigenerational exposure. © 2022 The Authors},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85137503128,

title = {Memory Impairment, Pro-Inflammatory Host Response and Brain Histopathologic Severity in Rats Infected with K. pneumoniae or P. aeruginosa Meningitis},

author = { B.H. Elwakil and B.A. Bakr and M.M. Aljeldah and N.S. Shehata and Y.H. Shahin and Z.A. Olama and M. Augustyniak and M.A.M. Aboul-Soud and A. El Wakil},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85137503128&doi=10.3390%2fpathogens11080933&partnerID=40&md5=eb232db4a06f262b995423c7aeb64cc1},

doi = {10.3390/pathogens11080933},

issn = {20760817},

year  = {2022},

date = {2022-01-01},

journal = {Pathogens},

volume = {11},

number = {8},

publisher = {MDPI},

abstract = {Meningitis caused by Klebsiella pneumoniae and Pseudomonas aeruginosa has lately become a prevalent cause of the central nervous system (CNS) infection. Bacterial invasion into the subarachnoid space prompts the releasing mechanism of chemokines and pro-inflammatory cytokines. The present study aimed to compare K. pneumoniae and P. aeruginosa meningitis concerning the memory, pro-inflammatory mediators and brain histopathological changes at different time intervals in adult Albino rats. The animals were sacrificed at three time intervals comprising 5, 10 and 15 days after meningitis induction. Cerebrospinal fluid (CSF) culture, relative brain weights, complete blood analysis, biochemical markers, levels of cytokine, chemokine and brain-derived neurotrophic factor (BDNF), neurotransmitter acetylcholine esterase (AChE) activity, and the brain histopathology of the infected rats in comparison to those in the control group were assessed. There was a significant increase in the levels of pro-inflammatory cytokines and chemokines including TNF-α, IL-1β, IL-6 and AChE after 5 days of bacterial meningitis infection with both K. pneumoniae and P. aeruginosa. The histopathological analysis of the cerebral cortex in the P. aeruginosa meningitis model at different time intervals revealed abundant numbers of dilated and congested blood vessels with severe hemorrhage, cerebral infarct, intracellular and extracellular vacuoles, and gliosis. Fifteen days post infection, a significant reduction in the brain tissue weight was observed. The meningitis model employing P. aeruginosa exhibited more evident time-dependent severity compared to K. pneumoniae, which may advocate its validity as a simple and effective research model to study meningitis of the CNS. This model may be utilized for further investigation to ascertain the molecular and biological association between bacterial meningitis and the development of the pathophysiological hallmarks underlying Alzheimer’s disease in preclinical and clinical setups. Clinical extrapolation based on studies employing animal disease models should be carefully interpreted. © 2022 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85116048656,

title = {Time-delayed effects of a single application of AgNPs on structure of testes and functions in Blaps polychresta Forskal, 1775 (Coleoptera: Tenebrionidae)},

author = { L.M. El-Samad and S. El-Ashram and H.K. Hussein and K.K. Abdul-Aziz and E.H. Radwan and N.R. Bakr and A. El Wakil and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116048656&doi=10.1016%2fj.scitotenv.2021.150644&partnerID=40&md5=0bf1d407c10696492b05980d4a97fdaf},

doi = {10.1016/j.scitotenv.2021.150644},

issn = {00489697},

year  = {2022},

date = {2022-01-01},

journal = {Science of the Total Environment},

volume = {806},

publisher = {Elsevier B.V.},

abstract = {Silver nanoparticles (AgNPs) are currently the most frequently used engineered nanoparticles. The penetration of AgNPs into ecosystems is undeniable, and their adverse effects on organism reproduction are of fundamental importance for ecosystem stability. In this study, the survival time of the Egyptian beetle Blaps polychresta Forskal, 1775 (Coleoptera: Tenebrionidae), after a single application of 7 different doses, was calculated for 30 days. Then, for the group for which the effect on mortality was calculated as LOAEL - the Lowest Observed Adverse Effect Level, namely, 0.03 mg AgNPs/g body weight (b.w.t.), the following were assessed: structure and ultrastructure of gonads by TEM and SEM, cell viability by cytometry, DNA damage by the comet assay, and a variety of stress markers by spectrophotometric methods. A dose-dependent reduction in the survival time of the insects was revealed. Detailed analysis of the testes of beetles treated with 0.03 mg AgNPs/g b.w.t. revealed numerous adverse effects of nanoparticles in structure and ultrastructure, accompanied by increased apoptosis (but not necrosis), increased DNA damage, increased lipid peroxidation, and decreased levels of antioxidant enzymes. Most likely, the observed results are connected with the gradual release of Ag+ from the surface of the nanoparticles, which, once applied, are internalized in cells and become a long-lasting, stable source of Ag+ ions. Thus, a single exposure to AgNPs may have the effects of chronic exposure and lead to structural damage and dysfunction of the gonads of B. polychresta. © 2021},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85135230527,

title = {Artichoke Leaf Extract-Mediated Neuroprotection against Effects of Aflatoxin in Male Rats},

author = { E.A. Ibrahim and M.I. Yousef and D.A. Ghareeb and M. Augustyniak and J.P. Giesy and M.A.M. Aboul-Soud and A. El Wakil},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135230527&doi=10.1155%2f2022%2f4421828&partnerID=40&md5=0ab5744830891f4b61d5e867f4879a42},

doi = {10.1155/2022/4421828},

issn = {23146133},

year  = {2022},

date = {2022-01-01},

journal = {BioMed Research International},

volume = {2022},

publisher = {Hindawi Limited},

abstract = {Attenuation of adverse effects of aflatoxin (AFB1) in brains of B1 rats by extracts of leaves of artichoke was studied. The active ingredients in extracts of leaves of artichoke, Cynara scolymus L., were determined by HPLC analysis. In the 42-day experiment, rats were exposed to either sterile water, 4% DMSO, 100 mg artichoke leaf extract/kg body mass, 72 μg aflatoxin B1/kg body mass, or AFB1 plus artichoke leaf extract. Neurotoxicity of AFB1 was determined by an increase in profile of lipids, augmentation of plasmatic glucose and concentrations of insulin, oxidative stress, increased activities of cholinergic enzymes, and a decrease in activities of several antioxidant enzymes and pathological changes in brain tissue. Extracts of artichoke leaf significantly reduced adverse effects caused by AFB1, rescuing most of the parameters to values similar to unexposed controls, which demonstrated that adverse, neurotoxic effects caused by aflatoxin B1 could be significantly reduced by simultaneous dietary supplementation with artichoke leaf extract, which itself is not toxic. © 2022 Enas A. Ibrahim et al.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85122652069,

title = {Cydalima perspectalis in Poland—8 Years of Invasion against the Background of Three Other Invasive Species},

author = { P.K. Bereś and P. Ziętara and M. Nakonieczny and Ł. Kontowski and M. Grzbiela and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122652069&doi=10.3390%2fd14010022&partnerID=40&md5=15b64fb470791391107c78cb3228b29a},

doi = {10.3390/d14010022},

issn = {14242818},

year  = {2022},

date = {2022-01-01},

journal = {Diversity},

volume = {14},

number = {1},

publisher = {MDPI},

abstract = {The box tree moth (Cydalima perspectalis) origins from East Asia. In Europe, it was recorded for the first time in 2007, and in Poland in 2012. By the end of 2020, it was found all over Poland. There are no published data on the range of C. perspectalis occurrence in Poland because it is not a quarantine pest in the European Union and is not subject to official monitoring. Data collected in 2018–2020 via a website dedicated to monitoring, for the first time, illustrate the current range and its largest concentrations in southern and central Poland. The monitoring confirmed that the main directions of the invasion are related to the main communication routes of Poland (south-north) and are of a long-distance character. The dispersal pattern corresponds to the model developed for Cameraria ohridella: a stratified dispersal model that considers long-distance road/rail transport. The second important factor contributing to the invasion of C. perspectalis are large human communities enabling rapid local dispersion (a diffusion model). Comparing its invasion with the monitoring data from 2007–2013 of two other invasive pests of Poland: Ostrinia nubilalis and Diabrotica virgifera, shows that a diffusion model best describes the spatial spread of these pests only to uninhabited neighboring areas. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85112577869,

title = {Multigenerational selection towards longevity changes the protective role of vitamin C against graphene oxide-induced oxidative stress in house crickets},

author = { B. Flasz and M. Dziewięcka and A. Kędziorski and M. Tarnawska and J. Augustyniak and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85112577869&doi=10.1016%2fj.envpol.2021.117996&partnerID=40&md5=7ef2db048ee15bffac23fcd838fd4ba7},

doi = {10.1016/j.envpol.2021.117996},

issn = {02697491},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Pollution},

volume = {290},

publisher = {Elsevier Ltd},

abstract = {This research was designed to investigate changes that can arise in an invertebrate organism due to stress caused by a strong prooxidant, graphene oxide (GO), and a potent antioxidant, vitamin C. The study aimed to investigate if vitamin C may support convalescence after chronic GO intoxication. We investigated the toxicity of chronic dietary graphene oxide administration in house cricket (Acheta domesticus) types: wild and selected for longevity (with a better developed antioxidant system; conducive to long life). Vitamin C was applied immediately after cessation of graphene oxide intoxication to check if it can support the remedial effect. The condition of cells, DNA stability, catalase activity, and the reproduction potential, measured as the Vitellogenin (Vg) protein expression level, were investigated in control and GO treated groups, recovery groups (-GO), and recovery groups with Vit. C (-GO + Vit.C). In this study vitamin C had no evident remedial effect on the house crickets exposed to graphene oxide. Most probably, the mechanism of vitamin C action, in case of intoxication with nanoparticles, is much more complicated. In the context of the results obtained, it is worth considering whether Vit. C, applied after GO intoxication, causes further disturbance of homeostasis in terms of the cells' redox potential. © 2021 Elsevier Ltd},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85105467182,

title = {Multigenerational graphene oxide intoxication results in reproduction disorders at the molecular level of vitellogenin protein expression in Acheta domesticus},

author = { B. Flasz and M. Dziewięcka and A. Kędziorski and M. Tarnawska and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105467182&doi=10.1016%2fj.chemosphere.2021.130772&partnerID=40&md5=412a7ee17abcd00614fdde8751e5fd1d},

doi = {10.1016/j.chemosphere.2021.130772},

issn = {00456535},

year  = {2021},

date = {2021-01-01},

journal = {Chemosphere},

volume = {280},

publisher = {Elsevier Ltd},

abstract = {The anthropogenic activities may lead to accumulation of graphene oxide (GO) pollution in the environment. Organisms exposed to chronic or multigenerational GO intoxication can present reproduction depletion. Vitellogenin (Vg) has been used as a parameter for evaluating female fertility due to its importance in embryo nutrition. In this study, we used a promising model organism, Acheta domesticus, which was intoxicated with GO in food for three generations. The aim of the study was to investigate the process of Vg synthesis in crickets depending on the exposure time, GO concentration, and age of the females. The results revealed that chronic GO intoxication had adverse effects on the Vg expression pattern. The 1st generation of insects showing low Vg expression was most affected. The 2nd generation of A. domesticus presented a high Vg expression. The last investigated generation seemed to cope with stress caused by GO, and the Vg expression was balanced. We suggest that the epigenetic mechanisms may play a role in the information transfer to the next generations on how to react to the risk factor and keep reproduction at a high rate. We suspect that chronic GO intoxication can disturb the regular formation of the Vg quaternary structure, resulting in consequences for developing an embryo. © 2021 Elsevier Ltd},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85106267328,

title = {Do nanoparticles cause hormesis? Early physiological compensatory response in house crickets to a dietary admixture of GO, Ag, and GOAg composite},

author = { R. Seyed Alian and M. Dziewięcka and A. Kędziorski and Ł. Majchrzycki and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106267328&doi=10.1016%2fj.scitotenv.2021.147801&partnerID=40&md5=1329bc1fff0f8d3dd59ba7eee545145c},

doi = {10.1016/j.scitotenv.2021.147801},

issn = {00489697},

year  = {2021},

date = {2021-01-01},

journal = {Science of the Total Environment},

volume = {788},

publisher = {Elsevier B.V.},

abstract = {This study aimed to identify the physiological responses of house cricket females following short-term exposure to relatively low dietary doses of graphene oxide (GO; 20 μg · g−1 food), silver (Ag; 400 μg · g−1 food) nanoparticles (NPs), or graphene oxide‑silver nanoparticle composite (GO-AgNPs; 20: 400 μg · g−1 food). Energy intake and distribution were measured on the third, sixth, and tenth day. A semi-quantitative API®ZYM assay of digestive enzyme fingerprints was performed on the third and tenth day of continuous treatment. Physicochemical properties of the NPs were obtained by combining SEM, EDX spectrometry, AFM, and DLS techniques. The obtained results showed decreased energy consumption, particularly assimilation as an early response to dietary NPs followed by compensatory changes in feeding activity leading to the same consumption and assimilation throughout the experimental period (10 days). The increased activities of digestive enzymes in NP-treated females compared to the control on the third day of the experiment suggest the onset of compensatory reactions of the day. Moreover, the insects treated with GO-AgNP composite retained more body water, suggesting increased uptake. The observed changes in the measured physiological parameters after exposure to NPs are discussed in light of hormesis. © 2021 The Authors},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85106147839,

title = {The structure–properties–cytotoxicity interplay: A crucial pathway to determining graphene oxide biocompatibility},

author = { M. Dziewięcka and M. Pawlyta and Ł. Majchrzycki and K. Balin and S. Barteczko and M. Czerkawska and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106147839&doi=10.3390%2fijms22105401&partnerID=40&md5=a45fcc37642a9b9fc64398e4a80f43a4},

doi = {10.3390/ijms22105401},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {10},

publisher = {MDPI},

abstract = {Interest in graphene oxide nature and potential applications (especially nanocarriers) has resulted in numerous studies, but the results do not lead to clear conclusions. In this paper, graphene oxide is obtained by multiple synthesis methods and generally characterized. The mechanism of GO interaction with the organism is hard to summarize due to its high chemical activity and variability during the synthesis process and in biological buffers’ environments. When assessing the biocompatibility of GO, it is necessary to take into account many factors derived from nanoparticles (structure; morphology; chemical composition) and the organism (species; defense mechanisms; adaptation). This research aims to determine and compare the in vivo toxicity potential of GO samples from various manufacturers. Each GO sample is analyzed in two concentrations and applied with food. The physiological reactions of an easy model Acheta domesticus (cell viability; apoptosis; oxidative defense; DNA damage) during ten-day lasting exposure were observed. This study emphasizes the variability of the GO nature and complements the biocompatibility aspect, especially in the context of various GO-based experimental models. Changes in the cell biomarkers are discussed in light of detailed physicochemical analysis. © 2021 by the author. Licensee MDPI, Basel, Switzerland.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85101456947,

title = {Stage-, sex- and tissue-related changes in H2O2, glutathione concentration, and glutathione-dependent enzymes activity in Aiolopus thalassinus (Orthoptera: Acrididae) from heavy metal polluted areas},

author = { E.A. Abdelfattah and M. Augustyniak and H.A. Yousef},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101456947&doi=10.1007%2fs10646-021-02354-0&partnerID=40&md5=1e2282f2e47a73ac5dfdcc7c4063a08e},

doi = {10.1007/s10646-021-02354-0},

issn = {09639292},

year  = {2021},

date = {2021-01-01},

journal = {Ecotoxicology},

volume = {30},

number = {3},

pages = {478-491},

publisher = {Springer},

abstract = {This study is part of a large project carried out at the Cairo University, Egypt, and focused on assessing physiological and biochemical changes in Aiolopus thalassinus under the influence of environmental pollution with heavy metals (Pb; Cd; Cu; and Zn). The study aimed to investigate parameters related to maintaining redox balance, with particular emphasis on stage-, sex- and tissue-dependent differences in H2O2 and glutathione (GSH) levels and activity of selected enzymes involved in GSH metabolism. A noticeable increase in the concentration of H2O2 was found, especially in the gut of 5th instar nymphs and females from the highly polluted site. An increase in GSH concentration was significant, especially in the gut of adult A. thalassinus from the high polluted site. However, recycling of reduced form of glutathione in the gut by glutathione reductase (GR) was relevant only for females from the high polluted site. Nymphs and females generally showed higher glutathione S-transferase (GST) activity, especially in the gut. These stage- and sex-related differences can result from different growth dynamic and various reproductive functions of nymphs and both sexes. The digestive track is in direct contact with xenobiotics consumed with food. Nymphs are characterized by vigorous growth, they feed intensively, and their development processes are associated with substantial oxygen consumption. Also, maintaining the antioxidant system at a high level can be more important for females than males due to egg production over a long period. It appears that de novo GSH synthesis is a favorable and cost-effective adaptation mechanism for A. thalassinus living in the high polluted site. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85102487461,

title = {Stress proteins concentration in caged Cyprinus carpio as a tool to monitor ecological stability in a model dam reservoir},

author = { A. Babczyńska and M. Tarnawska and P. Łaszczyca and P. Migula and B. Łozowski and A. Woźnica and I. Irnazarow and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102487461&doi=10.24425%2faep.2021.136452&partnerID=40&md5=5aafdbafbb462de53a29be6faf4a67ef},

doi = {10.24425/aep.2021.136452},

issn = {20834772},

year  = {2021},

date = {2021-01-01},

journal = {Archives of Environmental Protection},

volume = {47},

number = {1},

pages = {101-111},

publisher = {Polska Akademia Nauk},

abstract = {Variability of stress proteins concentration in caged carp exposed to transplantation experiment model dam reservoir was caused only by natural (climatic and biological) conditions. Thus, the reference data of stress proteins concentration range in young carp individuals were obtained. Metallothionein, HSP70 and HSP90 protein concentrations as biomarkers were assayed in the livers, gills and muscles of six-month-old (summer) or nine-month-old (autumn) carp individuals in relation to the site of encaging, season (summer or autumn), the term of sampling (1; 2 or 3 weeks after the transplantation) and tissue. Physicochemical analyses of the condition of water as well as pollution detection were conducted during each stage of the experiment. As the result of this study, the range of the variability of the stress protein concentration in young carp individuals was obtained. According to the analyses of the aquatic conditions of a reservoir with no detectable pollutants, we conclude that the variability in the stress protein concentration levels in the groups that were compared is solely the result of the natural conditions. Future regular monitoring of the reservoir using the transplantation method and young carp individuals will be both possible and reliable. Moreover, the range of variability in the stress protein concentrations that were measured in the young C. carpio individuals acquired from the model dam reservoir in relation to all of the studied factors may be applied in the monitoring of any other similar reservoir. © 2021. The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85091907971,

title = {Energy reserves, oxidative stress and development traits of Spodoptera exigua Hübner individuals from cadmium strain},

author = { A. Kafel and A. Babczyńska and A. Zawisza-Raszka and M. Tarnawska and A. Płachetka-Bożek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091907971&doi=10.1016%2fj.envpol.2020.115366&partnerID=40&md5=db2b849cc1b11998281831996100c168},

doi = {10.1016/j.envpol.2020.115366},

issn = {02697491},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Pollution},

volume = {268},

publisher = {Elsevier Ltd},

abstract = {Cadmium as a common environmental stressor may exert highly toxic effects on herbivorous insects. The question was whether possible elevation of an oxidative stress and imbalance of energetic reserves in insects may depend on developmental stage, sex and insect population's multigenerational history of exposure to cadmium. So, the aim of this study was to compare of the development traits, total antioxidant capacity, lipid peroxidation, RSSR to RSH ratio and the concentration of carbohydrates, glycogen, lipids and proteins in whole individuals (larvae or pupae) of Spodoptera exigua originating from two strains: control and selected over 120 generations with sublethal metal concentration (44 Cd mg per dry weight of diet). Generally, the increase of the protein, carbohydrates, glycogen concentration and lipid peroxidation decrease with age of the larvae were found. Revealed cases of a higher mobilisation of carbohydrates and proteins, and changes in total antioxidant capacity or lipid peroxidation, in individuals being under metal exposure, occurred in strain-depended mode. Short-term Cd exposure effect was connected with possible higher engagement of proteins and glycogen in detoxification processes, but also higher concentration of lipid peroxidation. In turn, for long-term Cd exposure effect lower lipids concentration and higher thiols usage seemed to be more specific. © 2020The study shown differences in defence strategies, energetic substances cumulation with development stages between insect pest representatives from strains differed in tolerance to cadmium. © 2020},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85097069721,

title = {Autophagy: a necessary defense against extreme cadmium intoxication in a multigenerational 2D experiment},

author = { A. Babczyńska and A. Nowak and A. Kafel and B. Łozowski and M.M. Rost-Roszkowska and M. Tarnawska and M. Augustyniak and M.K. Sawadro and A.E. Molenda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097069721&doi=10.1038%2fs41598-020-78316-z&partnerID=40&md5=7ec842d0f44a16b6784907a0728b2823},

doi = {10.1038/s41598-020-78316-z},

issn = {20452322},

year  = {2020},

date = {2020-01-01},

journal = {Scientific Reports},

volume = {10},

number = {1},

publisher = {Nature Research},

abstract = {Autophagy is a natural process that aims to eliminate malfunctioning cell parts, organelles or molecules under physiological conditions. It is also induced in response to infection, starvation or oxidative stress to provide energy in case of an energy deficit. The aim of this 2-dimensional study was to test if, and if so, how, this process depends on the concentration of cadmium in food (with Cd concentrations from 0 to 352 μg of Cd per g of food (dry weight)—D1 dimension) and the history of selection pressure (160 vs 20 generations of exposure to Cd—D2 dimension). For the study, the 5th instar larvae of a unique strain of the moth Spodoptera exigua that was selected for cadmium tolerance for 160 generations (44 μg of Cd per g of food (dry weight)), as well as 20-generation (11; 22 and 44 μg of Cd per g of food (dry weight)) and control strains, were used. Autophagy intensity was measured by means of flow cytometry and compared with life history parameters: survivability and duration of the 3rd larval stage. The highest values of autophagy markers were found in the groups exposed to the highest Cd concentration and corresponded (with a significant correlation coefficient) to an increased development duration or decreased survivorship in the respective groups. In conclusion, autophagy is probably initiated only if any other defense mechanisms, e.g., antioxidative mechanisms, are not efficient. Moreover, in individuals from pre-exposed populations, the intensity of autophagy is lower. © 2020, The Author(s).},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85091194234,

title = {Oxidative stress parameters, DNA damage and expression of HSP70 and MT in midgut of Trachyderma hispida (Forskål, 1775) (Coleoptera: Tenebrionidae) from a textile industry area},

author = { A.H. El-Gendy and M. Augustyniak and N.A. Toto and S. Al Farraj and L.M. El-Samad},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091194234&doi=10.1016%2fj.envpol.2020.115661&partnerID=40&md5=f5d9c6374031b5a06c23b143258f0dae},

doi = {10.1016/j.envpol.2020.115661},

issn = {02697491},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Pollution},

volume = {267},

publisher = {Elsevier Ltd},

abstract = {CapsuleTextile industry pollutants caused overexpression of stress proteins (HSP70) and metallothionein (MT) in T. hispida gut, but oxidative stress markers were inhibited. © 2020 Elsevier LtdThe textile mill industry is one of the major sources of pollution and contributors of metal contaminants to the environment. At the same time, the industry is important for global economy. Pollution caused by the textile industry is characteristic due to a unique set of potentially toxic substances. Darkling beetles (Coleoptera; Tenebrionidae), which live in all biogeographical regions, are especially common in soil quality and soil degradation studies. Our study was designed to assess long-term effects of textile industry (which generates specific pollution) on soil organisms, namely Trachyderma hispida. We especially wanted to find out what changes allow the species to survive and adapt to these specific conditions. Energy-dispersive X-ray spectroscopy of soil and midgut tissues of T. hispida sampled from a polluted site in the Edku textile industrial area in Egypt revealed a high accumulation of chemical elements, compared to a reference site. The concentration of elements in soil was well correlated with their concentration in the midgut of insects. Activity of superoxide dismutase, catalase, ascorbate peroxidase and glutathione S-transferase were negatively correlated with concentration of elements in soil and in the midgut. Meanwhile, malondialdehyde concentration in the midgut revealed an opposite tendency. DNA damage and expression of stress proteins, (HSP70 and metallothionein - MT) were elevated in insects from the polluted site. The activity of textile industry in the area of Edku undoubtedly causes an increase of soil pollution and, in consequence, causes a number of changes in the bodies of organisms living in these areas, including T. hispidus. Therefore, it is necessary to find a solution which limits the emission of waste from the textile industry, as well as to design modern strategies of processing, storing and utilizing it. © 2020 Elsevier Ltd},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85088645994,

title = {DNA damage in Spodoptera exigua after multigenerational cadmium exposure - A trade-off between genome stability and adaptation},

author = { M. Augustyniak and M. Tarnawska and M. Dziewięcka and A. Kafel and M.M. Rost-Roszkowska and A. Babczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088645994&doi=10.1016%2fj.scitotenv.2020.141048&partnerID=40&md5=8e46a0514984c19ad9d2fba14b49e9bf},

doi = {10.1016/j.scitotenv.2020.141048},

issn = {00489697},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {745},

publisher = {Elsevier B.V.},

abstract = {Human activity is a serious cause of extensive changes in the environment and a constant reason for the emergence of new stress factors. Thus, to survive and reproduce, organisms must constantly implement a program of adaptation to continuously changing conditions. The research presented here is focused on tracking slow changes occurring in Spodoptera exigua (Lepidoptera: Noctuidae) caused by multigenerational exposure to sub-lethal cadmium doses. The insects received food containing cadmium at concentrations of 5, 11, 22 and 44 μg per g of dry mass of food. The level of DNA stability was monitored by a comet assay in subsequent generations up to the 36th generation. In the first three generations, the level of DNA damage was high, especially in the groups receiving higher doses of cadmium in the diet. In the fourth generation, a significant reduction in the level of DNA damage was observed, which could indicate that the desired stability of the genome was achieved. Surprisingly, however, in subsequent generations, an alternating increase and decrease was found in DNA stability. The observed cycles of changing DNA stability were longer lasting in insects consuming food with a lower Cd content. Thus, a transient reduction in genome stability can be perceived as an opportunity to increase the number of genotypes that undergo selection. This phenomenon occurs faster if the severity of the stress factor is high but is low enough to allow the population to survive. © 2020 Elsevier B.V.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85086727685,

title = {Vitellogenin expression, DNA damage, health status of cells and catalase activity in Acheta domesticus selected according to their longevity after graphene oxide treatment},

author = { B. Flasz and M. Dziewięcka and A. Kędziorski and M. Tarnawska and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086727685&doi=10.1016%2fj.scitotenv.2020.140274&partnerID=40&md5=0dbe9ad21f1a92fd747f33cc48150904},

doi = {10.1016/j.scitotenv.2020.140274},

issn = {00489697},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {737},

publisher = {Elsevier B.V.},

abstract = {The increased use of graphene oxide (GO) raises worrisome questions regarding its possible threat to various ecosystems. Invertebrates represent valuable organisms for environmental studies. The lifespan can influence the ability to cope with toxins, especially those that act via oxidative stress. Two strains of Acheta domesticus, which are selected for longevity, were tested. The main aim was to investigate how GO, when administrated in food, affects: the condition of cells, DNA stability, ROS generation and the reproduction potential (the Vitellogenin (Vg) protein expression). The “recovery effect” – after removing GO from the diet for 15 days – was also measured. The results revealed different responses to GO in the wild (H) and long-living (D) strains. The D strain had a higher catalase activity compared to the H strain on the 25th day of the imago stage. Removing GO from the food resulted in a decrease in the catalase activity to the level of the control. On the 5th day of the imago stage, the H strain had a higher cell mortality than the D strain in the GO-intoxicated groups. There was more DNA damage in the H strain compared to the long-living strain. A remedial effect was seen after the GO was removed from the diet. The total Vg protein expression was higher in the H strain and lower in the D strain. The results indicated a GO concentration-dependent outcome. In both strains, removing the GO from the food led to a high Vg expression. The Vg expression after GO treatment, particularly translation and post-translational processing, should be studied in detail in the future. The D strain of crickets had more specialized mechanisms for maintaining homeostasis than the H strain. Organisms can fight off negative effects of GO, especially when they have systems that are well developed against oxidative stress. © 2020 Elsevier B.V.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85083788720,

title = {Graphene oxide as a new anthropogenic stress factor - multigenerational study at the molecular, cellular, individual and population level of Acheta domesticus},

author = { M. Dziewięcka and B. Flasz and M.M. Rost-Roszkowska and A. Kędziorski and A. Kochanowicz and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85083788720&doi=10.1016%2fj.jhazmat.2020.122775&partnerID=40&md5=4c5ac8dd6643714067dd963491c30c94},

doi = {10.1016/j.jhazmat.2020.122775},

issn = {03043894},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Hazardous Materials},

volume = {396},

publisher = {Elsevier B.V.},

abstract = {Although interest in transgenerational phenomena is constantly growing, little is known about the long-term toxicity of nanoparticles. In this study we investigate the multigenerational effects of graphene oxide (GO) which was given to Acheta domesticus in low doses (0.2; 2 and 20 μg·g-1 of food) for three subsequent generations. We assessed the influence of GO nanoparticles in many contexts, basing on parameters which represented different levels of biological organization: activity of antioxidant enzymes, level of apoptosis, DNA damage, histological analysis, hatching abilities, body mass and body length of insects, as well as their survival rate. The results have shown that exposing insects to nanoparticles over an extended period of time causes surprising intergenerational effects, based on significant differences in the life cycle and reproductive processes, which are not always dose-dependent. The second generation of insects appeared as the most unstable among the parameters that were studied, and did not match trends and patterns in the first and third generation categories. An increase of DNA damage was observed, but only in the third generation. This reduction of genome stability can be perceived as an essential element of adaptation, leading to an increase of genotype variants, which then undergo selection. © 2020 Elsevier B.V.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85072055835,

title = {Adhesion abilities and biosorption of Cd and Mg by microorganisms - first step for eco-friendly beneficiation of phosphate ore},

author = { H. Rabia and M. Ould Hamou and K. Kasperkiewicz and J. Brożek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072055835&doi=10.1038%2fs41598-019-49406-4&partnerID=40&md5=c2448f69f61e1c4d9fc49a13441d188c},

doi = {10.1038/s41598-019-49406-4},

issn = {20452322},

year  = {2019},

date = {2019-01-01},

journal = {Scientific Reports},

volume = {9},

number = {1},

publisher = {Nature Publishing Group},

abstract = {Chemical reagents used in traditional mineral processing can be toxic and hazardous for the environment. Therefore, the use of biotechnological methods is becoming increasingly important. Great hopes are being placed in the use of microorganisms for bio-beneficiation of raw materials. However, assessment of adhesion abilities of bacteria onto minerals surface as well as biosorption of metals are essential steps before designing final process of each ore beneficiation. The main aim of this work was an investigation of biosorption of Cd and Mg, as well as adhesion abilities of five microorganism species with minerals included in the natural mixture of phosphate ore form Djebel Onk, Algeria. The ore, due to its unique composition, created conditions for adhesion of all five tested microbial strains onto apatite surface during incubation at pH 3. Moreover, Rhodococcus erythropolis CD 130, Pseudomonas fluorescens and Escherichia coli adhered distinctly onto apatite surface during incubation at pH 7. Incubation lasting 20 min at pH 4-6 created the most favorable conditions for biosorption of metals by B. subtilis and adhesion of cells. In case of C. albicans, biosorption of metals as well as adhesion of cells onto the mineral surface were more effective after longer time and in a wider pH range. © 2019, The Author(s).},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85068259796,

title = {Protective role of zinc in Spodoptera exigua larvae under 135-generational cadmium exposure},

author = { M. Tarnawska and A. Babczyńska and K. Hassa and A. Kafel and A. Płachetka-Bożek and J. Augustyniak and M. Dziewięcka and B. Flasz and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068259796&doi=10.1016%2fj.chemosphere.2019.06.209&partnerID=40&md5=f08e57e4cdd129a976f96151aaf01fb5},

doi = {10.1016/j.chemosphere.2019.06.209},

issn = {00456535},

year  = {2019},

date = {2019-01-01},

journal = {Chemosphere},

volume = {235},

pages = {785-793},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to investigate whether zinc supplementation modulates cadmium toxicity in the beet armyworm Spodoptera exigua selected for 135 generations towards cadmium tolerance. To achieve this, larvae originating from three laboratory populations of S. exigua (control strain - C; cadmium-intoxicated for 135 generations strain - Cd; and control strain intoxicated with Cd for 1 generation - CCd) were additionally exposed to zinc in three concentrations (Zn1; 400 μg Zn·g−1 dry mass of food; Zn2; 200 μg Zn·g−1 dry mass of food; Zn3; 100 μg Zn·g−1 dry mass of food). As the markers of toxicity, a life history traits (the duration of L4 and L5 stages), cellular (DNA damage indices) and biochemical parameters (ADP/ATP ratio and ATP and HSP70 concentrations) were chosen. The duration of larval stages of Zn supplemented larvae was prolonged, while cellular and biochemical indicators, in general, appeared to be lower in comparison to the insects from respective reference groups in each laboratory populations. Moreover, the range of the differences depended on zinc concentration in food. We can suspect that zinc supplementation contributed to the protection of S. exigua individuals against negative effects of cadmium intoxication, probably at the cost of growth rate. Significant differences in the response pattern between insects from different laboratory populations indicate that the influence of additional stress factors is dependent on the overall condition of animals and their previous adaptation to other stressors. © 2019 Elsevier Ltd},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85064267438,

title = {Microevolution or wide tolerance? Level of stress proteins in the beet armyworm Spodoptera eqigua hübner (Lepidoptera: Noctuidae) exposed to cadmium for over 150 generations},

author = { M. Tarnawska and A. Kafel and M. Augustyniak and M.M. Rost-Roszkowska and A. Babczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064267438&doi=10.1016%2fj.ecoenv.2019.04.017&partnerID=40&md5=5f3adb3246bf4dcbd5777ca3f52fe722},

doi = {10.1016/j.ecoenv.2019.04.017},

issn = {01476513},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {178},

pages = {1-8},

publisher = {Academic Press},

abstract = {The aim of this study was to investigate whether the cadmium tolerance developed in the beet armyworm Spodoptera exigua selected for over 150 generations may be related to synthesis of the stress proteins metallothioneins (Mts) and 70 kDa heat shock proteins (HSP70). To achieve this, six S. exigua strains (control; k), 150-generation Cd exposure strain (cd), and four 18-generation Cd exposure strains differing in Cd concentration (cd44; cd22; cd11; cd5) were reared. Stress protein level was measured in the midgut of the 5th larval stage after 1–6, 12 and 18 generations. Cd contents was measured in the pupae. Unlike Cd concentration, which depended on metal contents in food but was not generation-dependent, the pattern of Mts and HSP70 concentrations changed in experimental strains from generation to generation. Stress protein levels in the insects exposed to the highest Cd concentration (the same as in the 150-generation Cd exposure strain), initially higher than in the control strain, after the 12th generation did not differ from the level measured in the control strains. It seems therefore that stress proteins play a protective role in insects of lower tolerance to cadmium. The tolerance developed during multigenerational exposure probably relies on mechanisms other than Mt and HSP70 synthesis. © 2019 Elsevier Inc.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85062391000,

title = {Immune response of juvenile common carp (Cyprinus carpio L.) exposed to a mixture of sewage chemicals},

author = { M. Tarnawska and M. Augustyniak and P. Łaszczyca and P. Migula and I. Irnazarow and M. Krzyżowski and A. Babczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062391000&doi=10.1016%2fj.fsi.2019.02.049&partnerID=40&md5=d42712874f989fa17f5c77ff077e7bc5},

doi = {10.1016/j.fsi.2019.02.049},

issn = {10504648},

year  = {2019},

date = {2019-01-01},

journal = {Fish and Shellfish Immunology},

volume = {88},

pages = {17-27},

publisher = {Academic Press},

abstract = {Pharmaceuticals and household chemicals are important components of municipal sewage. Many of them are biologically active, disrupting not only hormonal regulation of aquatic animals but also, indirectly, disturbing their immunological protection. In the environment, chemicals rarely act as individual substances, but as elements of mixtures. Therefore, the aim of this study was to check whether the acute laboratory exposure of common carp juveniles to a mixture of ibuprofen, sodium dodecyl sulphate (SDS), dimethyl sulfoxide (DMSO) and 17 α-ethynylestradiol in increasing concentrations, modifies the levels of innate immunity (lysozyme; C-reactive protein) as well as general stress (metallothioneins; heat shock proteins HSP70) markers in brain, liver, gills, spleen and mucus. The levels of the markers were measured by an immunodetection technique. Not only do the pharmaceuticals and household chemicals impair immunological reactions of young carp in various tissues but also do that in a concentration-dependent manner in the liver, gills, spleen and mucus. This has a very important implication, since it may result in higher sensitivity of young fish to pathogens due to energy allocation to defence processes. The comparisons of the pattern of stress reactions in the studied organ samples indicated that mucus appeared to be a good, non-invasive material for monitoring of environmental state and fish conditions. © 2019},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85058393591,

title = {Antioxidant enzyme activity in responses to environmentally induced oxidative stress in the 5th instar nymphs of Aiolopus thalassinus (Orthoptera: Acrididae)},

author = { H.A. Yousef and E.A. Abdelfattah and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058393591&doi=10.1007%2fs11356-018-3756-6&partnerID=40&md5=d78f6c83a7b15df82b3f4c8829bf77c5},

doi = {10.1007/s11356-018-3756-6},

issn = {09441344},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {4},

pages = {3823-3833},

publisher = {Springer Verlag},

abstract = {The response of antioxidant enzymes to oxidative environmental stress was determined in 5th instar nymphs of Aiolopus thalassinus (Orthoptera: Acrididae) collected from sites with different level of pollution with heavy metals, PO 4 3− , and SO 4 2− . The high polluted site induced higher DNA damage to individuals compared to the control site. The highest values of tail length (TL), tail moment (TM), and percent of DNA in tail (TDNA) were found in the gut of 5th instar nymphs from a high polluted site. Also, protein carbonyls and lipid peroxide levels were significantly higher in insects collected from polluted sites compared to those from the control site. A strong positive correlation between both protein carbonyl and lipid peroxide concentration and the pollution level of the sites was found in all tissues of the insects. The activity of superoxide dismutase (SOD) in the brain of insects collected from the high polluted site was significantly higher than that in the thoracic muscles and gut. We observed strong inhibition of catalase (CAT) activity. This effect was apparently caused by pollutants present at the high polluted site. The level of pollution significantly influenced polyphenol oxidase (PPO) activity in A. thalassinus nymphs in all examined tissues. The highest values were observed in the brain. The relationship between pollution and ascorbate peroxidase (APOX) activity in the examined tissues had no clear tendency. However, the lowest APOX activity was observed in individuals from the low polluted site. Level of pollution of sampling sites, oxidative stress biomarkers, and enzymatic response in A. thalanthsis 5th instar were negatively or positively correlated. Oxidative damage parameters, especially the percent of severed cells, lipid peroxides, and the activity of APOX, can be perceived as good markers of environmental multistress. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85053781876,

title = {Reproduction and development of Spodoptera exigua from cadmium and control strains under differentiated cadmium stress},

author = { A. Płachetka-Bożek and A. Kafel and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053781876&doi=10.1016%2fj.ecoenv.2018.09.016&partnerID=40&md5=714ca7792caf5b8727082b44352ae4a6},

doi = {10.1016/j.ecoenv.2018.09.016},

issn = {01476513},

year  = {2018},

date = {2018-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {166},

pages = {138-145},

publisher = {Academic Press},

abstract = {The growth and development of living organisms is programmed in genes, but exogenous factors (e.g. cadmium) may modulate endogenous information. Heavy metals may disturb physiological functions and accumulate in the tissues. The insects under prolonged heavy metal stress show some modifications in their metabolism management. The aim of this study was to compare the reproduction and development between individuals of S. exigua from the strain, exposed over 130 generations to sublethal concentration of cadmium (44 mg Cd/kg dry weight of larval diet), and the individuals from the control strain, both additionally exposed to different concentration of cadmium (22-704 mg Cd/kg dry weight of larval diet). The exposure to various cadmium concentrations in the diet revealed survival difference between the cadmium and the control animals at the larvae stage. The differences between adults were not evident. The telomere length (responsible for the duration of a lifespan) in the cadmium strain was shorter in the females than in the males and the individuals from the control strain. TERF1 gene expression (indirectly responsible for the telomere length) was higher in the individuals from the cadmium strain 24 hrs after eclosion. The significant reduction in the larvae body mass was observed in both strains, when the metal concentration was equal to or higher than 264 mg/kg dry weight of larval diet. The EC50 values (defined as of body mass loss), calculated 48 hours after cadmium exposure of individuals from control and cadmium strains, were respectively 632 and 725 mg Cd/kg dry weight of diet. However, some difference in reproduction (the total number of eggs laid and the oviposition time) between the strains appeared only in the groups fed on the uncontaminated diet. The control females laid almost two times more eggs than those from the cadmium strain, and the control ones had more than two times longer oviposition time than the females from the cadmium strain. The fluctuation was also noted in the size of eggs and the hatching success on the following days when both strains were compared, while the hatching success was higher for the insects from the cadmium strain. In conclusion, the insects from the cadmium strain are more resistant to cadmium contamination, as it is evidenced by the EC50 parameter. However, the females from the cadmium strain start laying eggs statistically later, have shorter telomeres and slightly reduced TERF1 gene expression, but hutching success in the strain is significantly higher when compared with the control individuals. © 2018 Elsevier Inc.},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85049328870,

title = {Chronic toxicity of nanodiamonds can disturb development and reproduction of Acheta domesticus L},

author = { J. Karpeta-Kaczmarek and A. Kędziorski and M.A. Augustyniak-Jabłokow and M. Dziewięcka and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049328870&doi=10.1016%2fj.envres.2018.05.027&partnerID=40&md5=b4a7a9e96fecc2fbc5e750ec4afab8f1},

doi = {10.1016/j.envres.2018.05.027},

issn = {00139351},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Research},

volume = {166},

pages = {602-609},

publisher = {Academic Press Inc.},

abstract = {The use of nanodiamonds in numerous materials designed for industry and medicine is growing rapidly. Consequently health and environmental risks associated with the exposure of humans and other biota to nanodiamonds-based materials are of the utmost importance. Scarcity of toxicological data for these particles led us to examine the potentially deleterious effects of nanodiamonds in model insect species, Acheta domesticus (Orthoptera) chronically exposed to ND in its diet. Organism-level end-point indices (lifespan; body weight; consumption; caloric value of faeces; reproduction) revealed adverse changes in the treated crickets in comparison with the control. Preliminary studies of oxidative stress level in the offspring of ND-treated crickets suggest toxicity of these particles limited to the exposed individuals. EPR analysis showing increase of radical signal in the faeces of ND-fed crickets led us to propose novel mechanism of nanodiamonds toxicity that is discussed in the light of literature data. Capsule: Development and reproduction of Acheta domesticus can be disturbed by the chronic exposure to nanodiamonds © 2018 Elsevier Inc.},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85045675947,

title = {Reduced fecundity and cellular changes in Acheta domesticus after multigenerational exposure to graphene oxide nanoparticles in food},

author = { M. Dziewięcka and P. Witas and J. Karpeta-Kaczmarek and J. Kwaśniewska and B. Flasz and K. Balin and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045675947&doi=10.1016%2fj.scitotenv.2018.04.207&partnerID=40&md5=11f8f54ea5f79c454f1fdecc030a2fb1},

doi = {10.1016/j.scitotenv.2018.04.207},

issn = {00489697},

year  = {2018},

date = {2018-01-01},

journal = {Science of the Total Environment},

volume = {635},

pages = {947-955},

publisher = {Elsevier B.V.},

abstract = {Despite the fact that the demand for graphene and its derivatives in commercial applications is still growing, many aspects of its toxicity and biocompatibility are still poorly understood. Graphene oxide, which is released into the environment (air; soil and water) as so-called nanowaste or nanopollution, is able to penetrate living organisms. It is highly probable that, due to its specific nature, it can migrate along food chains thereby causing negative consequences. Our previous studies reported that short-term exposure to graphene oxide may increase the antioxidative defense parameters, level of DNA damage, which results in numerous degenerative changes in the gut and gonads. The presented research focuses on reproductive dysfunction and cellular changes in Acheta domesticus after exposure to GO nanoparticles in food (concentrations of 20 and 200 μg·g−1 of food) throughout their entire life cycle. The results showed that long-term exposure to GO caused a significant decrease in the reproductive capabilities of the animals. Moreover, the next generation of A. domesticus had a lower cell vitality compared to their parental generation. It is possible that graphene oxide can cause multigenerational harmful effects. © 2018 Elsevier B.V.},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85028955528,

title = {Molecular changes in vitellogenin gene of Spodoptera exigua after long-time exposure to cadmium – Toxic side effect or microevolution?},

author = { A. Płachetka-Bożek and K. Chwiałkowska and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028955528&doi=10.1016%2fj.ecoenv.2017.08.067&partnerID=40&md5=9cff3d19356bfb0cbdda06ae8ef1bfd6},

doi = {10.1016/j.ecoenv.2017.08.067},

issn = {01476513},

year  = {2018},

date = {2018-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {147},

pages = {461-470},

publisher = {Academic Press},

abstract = {The reproduction of pest insects is a continuously ongoing issue, especially in the environmental pollution context. Natural or artificial stressing factors enforce a kind of trade-off, most often between growth/survival and reproduction, which improves fitness of the organism. Harmful factors, such as cadmium, can affect the vitellogenesis leading to reduction of yolk synthesis and egg production. The aim of this study was to assess whether 130-generational selection to cadmium in food might have induced change in vitellogenesis of Spodoptera exigua. We analyzed the level of Vg gene expression in S. exigua from the control and the cadmium strain at regular time intervals within 48 h after eclosion. The full sequence of Vg gene was also compared between strains. The vitellogenin gene expression in both strains was time-dependent. This dependence was more visible in the control strain. In the cadmium strain the vitellogenin expression was significantly lower, comparing with the control strain in the first day after eclosion but increased significantly in the second day. The sequenced CDS (5286 bp long) of the control and the cadmium strains were translated into protein sequences containing both 1761 aa. The protein sequences comparison revealed that there is one amino acid change at aa position 1282. Multiple alignments of six orthologous proteins from different species showed that amino acid change is located in the conserved position. Long-lasting exposure to cadmium resulted in permanent mutation in vitellogenin gene. We do not know yet if the mutation can improve fitness of the cadmium-selected insects. However, we can suppose that the mutation is neutral or even beneficial. The mutation and most probably additional effects of cadmium exposure have an influence on the vitellogenin expression. Some modification in the expression of the vitellogenin receptor are also likely to be important. © 2017 Elsevier Inc.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85031894354,

title = {Cross tolerance in beet armyworm: long-term selection by cadmium broadens tolerance to other stressors},

author = { M. Augustyniak and M. Tarnawska and A. Babczyńska and A. Kafel and A. Zawisza-Raszka and B. Adamek and A. Płachetka-Bożek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031894354&doi=10.1007%2fs10646-017-1865-5&partnerID=40&md5=17ff4c7a2acb645b6758e1bf3f98a15d},

doi = {10.1007/s10646-017-1865-5},

issn = {09639292},

year  = {2017},

date = {2017-01-01},

journal = {Ecotoxicology},

volume = {26},

number = {10},

pages = {1408-1418},

publisher = {Springer New York LLC},

abstract = {Long lasting exposure of animals to stressing factor may lead to the selection of population able to cope with the stressor at lower cost than unexposed individuals. The aim of this study was to assess whether 130-generational selection of a beet armyworm to cadmium in food might have induced tolerance also to other stressors. The potential tolerance was assessed by means of unspecific stress markers: HSP70 concentration, DNA damage level, and energy budget indices in L5 larval instars of beet armyworm. The animals originated from Cd-exposed and control strains exposed additionally in a short-term experiment to high/low temperature or pesticide—spinosad. The application of the additional stressors caused, in general, an increase in the levels of studied parameters, in a strain-dependent manner. The most significant increase was found in HSP70 level in the individuals from the Cd-strain exposed to various spinosad concentration. Therefore, multigenerational contact with cadmium caused several changes that enable the insect to survive under a chronic stress, preparing the organism to the contact with an additional, new stressor. This relationship may be described as a sort of cross tolerance. This may, possibly, increase the probability of population survivorship and, at the same time, decrease the efficiency of pesticide-based plant protection efforts. © 2017, The Author(s).},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85027874562,

title = {Evaluation of Candidate Reference Genes for Quantitative Gene Expression Analysis in Spodoptera exigu a after Long-time Exposure to Cadmium},

author = { A. Płachetka-Bożek and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027874562&doi=10.1038%2fs41598-017-08630-6&partnerID=40&md5=a24a9863adfa59226f3453b1fb509780},

doi = {10.1038/s41598-017-08630-6},

issn = {20452322},

year  = {2017},

date = {2017-01-01},

journal = {Scientific Reports},

volume = {7},

number = {1},

publisher = {Nature Publishing Group},

abstract = {Studies on the transcriptional control of gene expression play an important role in many areas of biology. Reference genes, which are often referred to as housekeeping genes, such as GAPDH, G3PDH, EF2, RpL7A, RpL10, TUBα and Actin, have traditionally been assumed to be stably expressed in all conditions, and they are frequently used to normalize mRNA levels between different samples in qPCR analysis. However, it is known that the expression of these genes is influenced by numerous factors, such as experimental conditions. The difference in gene expression underlies a range of biological processes, including development, reproduction and behavior. The aim of this study was to show the problems associated with using reference genes in the qPCR technique, in a study on inbred strains of Spodoptera exigua selected toward cadmium resistance. We present and discuss our results and observations, and give some recommendations concerning the use and limitations of housekeeping genes as internal standards, especially in research on insects. Our results suggest that holometabolism and poikilothermia, as well as time since metamorphosis and the level of exposure to the selective factor (cadmium in this case), have a significant effect on the expression of reference genes. © 2017 The Author(s).},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85028554985,

title = {Erratum to: Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats (Journal of Obesity Surgery, (2015), 25, (1848–1857), 10.1007/s11695-015-1618-6)},

author = { T. Sawczyn and D. Stygar and I. Karcz-Socha and J. Fink and B. Skrzep-Poloczek and M. Kłosok and B. Doleżych and M. Augustyniak and A. Zawisza-Raszka and M. Kukla and M. Michalski and A. Fimiarz and K. Żwirska-Korczala and W.K. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028554985&doi=10.1007%2fs11695-017-2892-2&partnerID=40&md5=8c8a040ca22deb930c123abe8c77ce0c},

doi = {10.1007/s11695-017-2892-2},

issn = {09608923},

year  = {2017},

date = {2017-01-01},

journal = {Obesity Surgery},

volume = {27},

number = {10},

pages = {2780-},

publisher = {Springer New York LLC},

abstract = {In the original article, the name of the authorWojciech Konrad Karcz was incorrect—it is correct here. © Springer Science+Business Media, LLC 2017.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85017565381,

title = {Lifespan differences between queens and workers are not explained by rates of molecular damage},

author = { E.R. Lucas and M. Augustyniak and A. Kędziorski and L. Keller},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017565381&doi=10.1016%2fj.exger.2017.03.008&partnerID=40&md5=7ca8f6159e66be170a28d1ca2bf202c0},

doi = {10.1016/j.exger.2017.03.008},

issn = {05315565},

year  = {2017},

date = {2017-01-01},

journal = {Experimental Gerontology},

volume = {92},

pages = {1-6},

publisher = {Elsevier Inc.},

abstract = {The biological processes that underlie senescence are of universal biological importance, yet they remain poorly understood. A popular theory proposes that senescence is the result of limited investment into mechanisms involved in the prevention and repair of molecular damage, leading to an accumulation of molecular damage with age. In ants, queen and worker lifespans differ by an order of magnitude, and this remarkable difference in lifespan has been shown to be associated with differences in the expression of genes involved in DNA and protein repair. Here we use the comet assay and Western Blotting for poly-ubiquitinated proteins to explore whether these differences in expression lead to differences in the accumulation of DNA damage (comet assay) or protein damage (protein ubiquitination) with age. Surprisingly, there was no difference between queens and workers in the rate of accumulation of DNA damage. We also found that levels of ubiquitinated proteins decreased with age, as previously reported in honeybees. This is in contrast to what has been found in model organisms such as worms and flies. Overall, these results reveal that the link between investment into macromolecular repair, age-related damage accumulation and lifespan is more complex than usually recognised. © 2017 Elsevier Inc.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85016115768,

title = {Erratum to: Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats (Obesity Surgery, (2015), 25, 10, (1848-1857), 10.1007/s11695-015-1618-6)},

author = { T. Sawczyn and D. Stygar and I. Karcz-Socha and J. Fink and B. Skrzep-Poloczek and M. Kłosok and B. Doleżych and M. Augustyniak and A. Zawisza-Raszka and M. Kukla and M. Michalski and A. Fimiarz and K. Żwirska-Korczala and K.K. Wojciech},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016115768&doi=10.1007%2fs11695-015-1964-4&partnerID=40&md5=ac4ea540b9123adb6235f56130b156a2},

doi = {10.1007/s11695-015-1964-4},

issn = {09608923},

year  = {2017},

date = {2017-01-01},

journal = {Obesity Surgery},

volume = {27},

number = {6},

pages = {1640-},

publisher = {Springer New York LLC},

abstract = {In the original article the author names were presented incorrectly. They are correct here. © 2016, Springer Science+Business Media New York.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85010734928,

title = {Evaluation of oxidative stress biomarkers in Aiolopus thalassinus (Orthoptera: Acrididae) collected from areas polluted by the fertilizer industry},

author = { H.A. Yousef and E.A. Abdelfattah and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010734928&doi=10.1007%2fs10646-017-1767-6&partnerID=40&md5=232ed29717710dc17f17e396b7803b50},

doi = {10.1007/s10646-017-1767-6},

issn = {09639292},

year  = {2017},

date = {2017-01-01},

journal = {Ecotoxicology},

volume = {26},

number = {3},

pages = {340-350},

publisher = {Springer New York LLC},

abstract = {The waste products of the fertilizer industry such as heavy metals, but especially phosphates and sulphates, are a serious problem that influences the structure and functioning of ecosystems. The levels of Cd, Pb, Zn, Cu, sulphates and phosphates were measured in soil samples from four sites: a control and sites that were 1, 3 and 6 km (sites A–C) away from the Abu-Zaabal Fertilizer Company. Oxidative stress markers (protein carbonyls; lipid peroxides), antioxidant enzymes (superoxide dismutase (SOD); catalase (CAT); polyphenoloxidase (PPO) and ascorbate peroxidase (APOX)) were evaluated in the tissues of Aiolopus thalassinus, collected from the corresponding sites. The highest concentrations of Cu and Zn were found in the soil from site A. The level of protein carbonyls in the brain, thoracic muscles and gut of the males and females from sites A, B and C were 11.82, 4.38, 5.97 (males) and 19.04, 16.65, 7.79 (females) times higher, respectively, compared to the individuals from the control site. Lipid peroxides levels in both sexes were significantly correlated with the distance from the source of the contamination. In the brain, thoracic muscles and gut of the males and females collected from site A, the level of lipid peroxides were 15.41, 23.49, 11.50 (males) and 25.36, 11.34, 15.37 (females) times higher compared to the values of the control animals. The activities of SOD, PPO, CAT and APOX were significantly affected by the environmental pollutants. The enzymatic and non-enzymatic oxidative markers in the Aiolopus thalassinus, a common insect species that inhabits various ecosystems, can also be used as a relevant biomarker of the pollution that is caused by the fertilizer industry. © 2017, Springer Science+Business Media New York.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85019394786,

title = {Biomonitoring of genotoxicity of industrial fertilizer pollutants in Aiolopus thalassinus (Orthoptera: Acrididae) using alkaline comet assay},

author = { E.A. Abdelfattah and M. Augustyniak and H.A. Yousef},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019394786&doi=10.1016%2fj.chemosphere.2017.05.082&partnerID=40&md5=c7a8aea8e4c08a2521ceeca18eb08110},

doi = {10.1016/j.chemosphere.2017.05.082},

issn = {00456535},

year  = {2017},

date = {2017-01-01},

journal = {Chemosphere},

volume = {182},

pages = {762-770},

publisher = {Elsevier Ltd},

abstract = {Phosphate fertilizer industry is considered as one of the main sources of environmental pollutants. Besides solid waste products, e.g. phosphates, sulphates, and heavy metals, also atmospheric pollutants, such as hydrofluoric acid fumes (HF), sulphur dioxide (SO2), nitrogen oxides (NO2), and particulate matter with diameter up to 10 μm (PM10) can be dangerous. Genotoxic effect of these pollutants was monitored by assessing the DNA damage using alkaline comet assay on cells from brain, thoracic muscles and gut of Aiolopus thalassinus collected at three sites (A-C) located at 1, 3, and 6 km away from Abu-Zaabal Company for Fertilizers and Chemical Industries. Control site was established 32 km from the source of pollution, at the Cairo University Campus. The level of the DNA damage was significantly higher in insects from polluted sites comparing to that from the control site. A strong negative correlation between percentage of cells with visible DNA damage (% of severed cells) and the distance of the sites from Abu-Zaabal Company was found. The best parameter for monitoring of fertilizer pollutants is % of severed cells. Possible impact of Abu-Zaabal Company (extremely high concentration of phosphates and sulphates in all the polluted sites) on DNA integrity in A. thalassinus tissues was discussed. The potential use of the comet assay as a biomonitoring method of the environmental pollution caused by fertilizer industry was proposed. Specific pollution resulting from the activity of the fertilizer industry can cause comparable adverse effects in the organisms inhabiting areas up to 6 km from the source of contamination. © 2017 Elsevier Ltd},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85009157214,

title = {Short-term in vivo exposure to graphene oxide can cause damage to the gut and testis},

author = { M. Dziewięcka and J. Karpeta-Kaczmarek and M. Augustyniak and M.M. Rost-Roszkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009157214&doi=10.1016%2fj.jhazmat.2017.01.012&partnerID=40&md5=ca2682854bc1a00b81f9cfc494e9ce7b},

doi = {10.1016/j.jhazmat.2017.01.012},

issn = {03043894},

year  = {2017},

date = {2017-01-01},

journal = {Journal of Hazardous Materials},

volume = {328},

pages = {80-89},

publisher = {Elsevier B.V.},

abstract = {Graphene oxide (GO) has unique physicochemical properties and also has a potentially widespread use in every field of daily life (industry; science; medicine). Demand for nanotechnology is growing every year, and therefore many aspects of its toxicity and biocompatibility still require further clarification. This research assesses the in vivo toxicity of pure and manganese ion-contaminated GO that were administrated to Acheta domesticus with food (at 200 mg kg−1of food) throughout their ten-day adult life. Our results showed that short-term exposure to graphene oxide in food causes an increase in the parameters of oxidative stress of the tested insects (catalase – CAT; total antioxidant capacity – TAC), induces damage to the DNA at a level of approximately 35% and contributes to a disturbance in the stages of the cell cycle and causes an increase of apoptosis. Moreover, upon analyzing histological specimens, we found numerous degenerative changes in the cells of the gut and testis of Acheta domesticus as early as ten days after applying GO. A more complete picture of the GO risk can help to define its future applications and methods for working with the material, which may help us to avoid any adverse effects and damage to the animal. © 2017 Elsevier B.V.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84999759002,

title = {Phenotypic plasticity, epigenetic or genetic modifications in relation to the duration of Cd-exposure within a microevolution time range in the beet armyworm},

author = { M. Augustyniak and A. Płachetka-Bożek and A. Kafel and A. Babczyńska and M. Tarnawska and A. Janiak and A. Loba and M. Dziewięcka and J. Karpeta-Kaczmarek and A. Zawisza-Raszka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84999759002&doi=10.1371%2fjournal.pone.0167371&partnerID=40&md5=71387ac76e18d04e4111760b53546841},

doi = {10.1371/journal.pone.0167371},

issn = {19326203},

year  = {2016},

date = {2016-01-01},

journal = {PLoS ONE},

volume = {11},

number = {12},

publisher = {Public Library of Science},

abstract = {In the case of the pests inhabiting metal polluted or fields where the use of pesticides is common, a natural selection of resistant individuals can occur. This may pose serious problems for humans, agriculture, as well as the economies of many countries. In this study, the hypothesis that multigenerational (120 generations) exposure to cadmium of a beet armyworm population could be a selecting factor toward a more efficient DNA protection was verified. The hemocytes of individuals from two culture strains (control and Cd-exposed) were treated with H2O2 (a DNA-damaging agent) or PBS (reference). The level of DNA damage was assessed using the Comet assay immediately and 5, 15 and 30 min. after the treatment. The immediate result of the contact with H2O2 was that the level of DNA damage in the hemocytes of the insects from both strains increased significantly. However, in the cells of the Cd-exposed individuals, the level of DNA damage decreased over time, while in the cells from the control insects it remained at the same level with no evidence of repair. These results suggest that efficient defense mechanisms may exist in the cells of insects that have prolonged contact with cadmium. Some evolutionary and trade-off aspects of the phenomenon are discussed. In a wider context, comparing the results obtained in the laboratory with field studies may be beneficial for understanding basic mechanisms of the resistance of an organism. To summarize, the high potential for the repair of DNA damage that was observed in the insects from the cadmium strain may confirm the hypothesis that multigenerational exposure to that metal may possibly contribute to the selection of insects that have a wider tolerance to oxidative stress. However, our investigations of polymorphism using AFLP did not reveal differences between the two main insect strains. © 2016 Augustyniak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84988967346,

title = {Effects of short-term exposure of Acheta domesticus to nanodiamonds in food: DNA damage but no histological alteration in tissues},

author = { J. Karpeta-Kaczmarek and M. Dziewięcka and M. Augustyniak and M.M. Rost-Roszkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988967346&doi=10.1016%2fj.carbon.2016.09.053&partnerID=40&md5=d32e762f3268d893f2f64f4d52fc9f33},

doi = {10.1016/j.carbon.2016.09.053},

issn = {00086223},

year  = {2016},

date = {2016-01-01},

journal = {Carbon},

volume = {110},

pages = {458-468},

publisher = {Elsevier Ltd},

abstract = {The aim of this work is to evaluate toxicity of nanodiamonds (ND; 20 and 200 mg g−1 food) to Acheta domesticus after short-term exposure (10 days) in food. Catalase (CAT), total antioxidant capacity (TAC), stress protein level (HSP70) and DNA damage to hemocytes were monitored every two days. On day 2 and 10, the levels of reactive oxygen species (ROS), apoptosis (annexin V and multicaspase tests), cell cycle and DNA damage to the gut were examined. The ultrastructure of the gut epithelium and testis was assessed on day 10 of the experiment. CAT, TAC, HSP70 in hemolymph, and histological morphology of gut and testis did not indicate ND toxicity. In the gut we observed a transient increase in CAT activity on day 2,4 and 6; and an increase in HSP70 level on day 6, 8 and 10. TAC level in the gut did not increase until day 10. ND did not influence ROS level in cells and the cell cycle. Yet, we observed a moderate increase in apoptosis, particularly at the higher ND concentration. ND induced dose-dependent DNA damage. We can conclude that short-term, occasional exposure to trace amounts of ND, like in medical applications, is probably safe for organisms. © 2016 Elsevier Ltd},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84994000474,

title = {Caffeine effects on AdoR mRNA expression in Drosophila melanogaster},

author = { J. Francikowski and B. Baran and A. Płachetka-Bożek and M. Krzyżowski and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84994000474&doi=10.1515%2fbiol-2016-0034&partnerID=40&md5=4ed7fa379ebd2558062e919116cadecf},

doi = {10.1515/biol-2016-0034},

issn = {23915412},

year  = {2016},

date = {2016-01-01},

journal = {Open Life Sciences},

volume = {11},

number = {1},

pages = {244-249},

publisher = {De Gruyter Open Ltd},

abstract = {In this study, we aimed to evaluate whether exposure to caffeine in the early stages of development affect AdoR mRNA expression levels in the fruit fly (Drosophila melanogaster) and how this will relate to the developmental success of flies. Adenosine receptors are seen as the most important biochemical targets of caffeine. Simultaneously adenosine signaling orchestrates the development and growth of insects. We demonstrate that AdoR mRNA expression in D. melanogaster is persistent from early stages till imago. Strong alterations in AdoR expression were observed in larvae that had been treated with caffeine. However, after the imaginal molt, the differences in AdoR expression between the insects from all of the test groups evened out despite a wide range of developmental success in the groups. Taken together, these results suggest that caffeine affects the expression of its cellular targets even from the early stages of fruit fly development and thus there is a significantly lower larvae-to-adult survival rate. Moreover, we also proved that the expression of AdoR undergoes a peculiar reset during the maturation of D. melanogaster despite the conditions in which larvae developed. © 2016 Jacek Francikowski et al.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84969195111,

title = {The level of DNA damage in adult grasshoppers Chorthippus biguttulus (Orthoptera, Acrididae) following dimethoate exposure is dependent on the insects' habitat},

author = { J. Karpeta-Kaczmarek and M. Kubok and M. Dziewięcka and T. Sawczyn and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84969195111&doi=10.1016%2fj.envpol.2016.05.032&partnerID=40&md5=e511b5840b4ddd60579c4cbed2d7cec4},

doi = {10.1016/j.envpol.2016.05.032},

issn = {02697491},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Pollution},

volume = {215},

pages = {266-272},

publisher = {Elsevier Ltd},

abstract = {The comet assay was used to study the DNA damage that was induced by dimethoate in the hemocyte cells of adult Chorthippus biguttulus grasshoppers (Insecta: Orthoptera) that originated from two sites with varying levels of pollution. The primary focus of the study was to examine whether continuous exposure to environmental stress can modify the effect of pesticides on genome stability. After three days of acclimation to laboratory conditions, the level of DNA damage in the hemocytes of Bow-winged grasshoppers was within a similar range in the insects from both areas. However, the level of DNA damage following dimethoate treatment was significantly higher in the insects from the reference area (Pogoria) than in the individuals from the heavily polluted location (Szopienice). Four hours after pesticide treatment, the Tail DNA (TDNA) in the hemocytes of the male and female specimens from Pogoria was as high as 75% and 50% respectively, whereas the values in males and females from Szopienice only reached 30% and 20%, respectively. A rapid decrease in DNA damage was observed in both populations 24 h after the pesticide application. The habitat of an insect (site), the administration of the dimethoate (treatment), and the period following the application of the pesticide (time), all significantly influenced the levels of DNA damage. No interactions related to TDNA were observed between the variables 'sex' and 'treatment'. Similarly, the variable 'sex', when analyzed alongside 'treatment' and 'site' (the area from which the insects were collected), or 'treatment' and 'time' had no influence on TL. Exposure to dimethoate undoubtedly contributed to the formation of DNA damage in the hemocytes of adult C. biguttulus. However, the level of damage was clearly dependent on the place where the insects were captured. © 2016 Elsevier Ltd.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85009155963,

title = {Oxidative stress and genotoxic effects of diamond nanoparticles},

author = { J. Karpeta-Kaczmarek and M. Dziewięcka and M. Augustyniak and M.M. Rost-Roszkowska and M. Pawlyta},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85009155963&doi=10.1016%2fj.envres.2016.03.033&partnerID=40&md5=bfdeb3e3583c5cd2075827dc02e209e2},

doi = {10.1016/j.envres.2016.03.033},

issn = {00139351},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Research},

volume = {148},

pages = {264-272},

publisher = {Academic Press Inc.},

abstract = {Due to the unique and useful properties of nanodiamonds (ND), their production and use is rapidly increasing. Thus, more of these particles will be released into the environment and organisms will inevitably be exposed to them. The current knowledge about the toxicity of ND, especially in vivo toxicity, is fragmentary. In this study, the toxicity of nanodiamonds was assessed in Acheta domesticus following chronic exposure to different nominal concentrations of ND (20 and 200 µg g−1 food) administrated in food for the entire lifespan. The activity of oxidative stress enzymes (catalase; glutathione peroxidase), total antioxidant capacity, as well as the level of heat shock protein were determined. A significant increase in all of the measured parameters was observed after seven weeks of exposure in individuals exposed to higher concentrations of ND (200 µg g−1 food). In animals exposed to lower concentrations of ND (20 µg g−1 food), there were few significant changes to these parameters. Analysis of DNA damage performed after fourteen weeks using the comet assay revealed DNA instabilities in the insects, especially the ones that had been exposed to the higher doses of ND. These findings may suggest that the toxicity of ND is concentration dependent. While high doses interact in a toxic manner, trace amounts, which are more likely in the environment, might be safe for organisms. Extreme caution should be taken when handling nanodiamonds. © 2016 Elsevier Inc.},

note = {25},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84961142729,

title = {Genotoxic effects of starvation and dimethoate in haemocytes and midgut gland cells of wolf spider Xerolycosa nemoralis (Lycosidae)},

author = { G. Wilczek and M. Mędrzak and M. Augustyniak and P. Wilczek and M. Stalmach},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961142729&doi=10.1016%2fj.envpol.2016.02.037&partnerID=40&md5=11c3eaaf35e46fc51bfbaa698db5c71f},

doi = {10.1016/j.envpol.2016.02.037},

issn = {02697491},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Pollution},

volume = {213},

pages = {370-378},

publisher = {Elsevier Ltd},

abstract = {The aim of this study was to assess the genotoxic effects of starvation and dimethoate (organophosphate insecticide) in female and male wolf spiders Xerolycosa nemoralis (Lycosidae) exposed to the stressors under laboratory conditions. DNA damage was measured in haemocytes and midgut gland cells using the comet assay. In response to the two stressing factors, both cell types showed %TDNA, tail length (TL) and OTM values higher in males than in females. Level of DNA damage in haemocytes was greater than in midgut gland cells. In both sexes, the strongest genotoxicity was recorded at single application of dimethoate. After five-time exposure to the pesticide, genotoxic effects of a single dose were sustained in males and reduced to the control level in females. Starvation stress was well tolerated by the females, in which neither cell type was affected by DNA damage. However, in male haemocytes food deprivation induced severe DNA damage, what suggests suppression of the defence potential at prolonged starvation periods. © 2016 Elsevier Ltd. All rights reserved.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84960146447,

title = {Ultrastructure of the gut epithelium in Acheta domesticus after long-term exposure to nanodiamonds supplied with food},

author = { J. Karpeta-Kaczmarek and M. Augustyniak and M.M. Rost-Roszkowska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960146447&doi=10.1016%2fj.asd.2016.02.002&partnerID=40&md5=b823973c49c0d6eb90b51665238d44bc},

doi = {10.1016/j.asd.2016.02.002},

issn = {14678039},

year  = {2016},

date = {2016-01-01},

journal = {Arthropod Structure and Development},

volume = {45},

number = {3},

pages = {253-264},

publisher = {Elsevier Ltd},

abstract = {The biosafety of nanoparticles and the potential toxicity of nanopollutants and/or nanowastes are all currently burning issues. The increased use of nanoparticles, including nanodiamonds (ND), entails the real risk of their penetration into food chains, which may result in the contamination of animal and, as a result, human food. Knowledge about changes in the ultrastructure of tissues in organisms that have been exposed to ND is still very limited. The aim of the study was to describe the ultrastructure of the gut epithelium in Acheta domesticus after exposure to different concentrations of ND (0; 20 or 200 μg g-1 - control; ND20 and ND200 groups; respectively) administered with food over a five-week period. The ultrastructure of the foregut, midgut and hindgut was assessed using Transmission Electron Microscopy (TEM). A number of changes in the structure of the gut in crickets that had consumed nanodiamond-contaminated food were observed. The epithelium of the midgut and hindgut were clearly damaged by ND, although the foregut was not affected. A positive relationship between the ND concentration in food and the degree of damage to the structure of epithelial cells was observed. Autophagy, especially mitophagy and reticulophagy, was activated in relation to the appearance of ND particles. A putative ND toxicity mechanizm is proposed. Extreme caution should be maintained when using nanodiamonds on a large scale. © 2016 Elsevier Ltd.},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84948844325,

title = {Evaluation of in vivo graphene oxide toxicity for Acheta domesticus in relation to nanomaterial purity and time passed from the exposure},

author = { M. Dziewięcka and J. Karpeta-Kaczmarek and M. Augustyniak and Ł. Majchrzycki and M.A. Augustyniak-Jabłokow},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84948844325&doi=10.1016%2fj.jhazmat.2015.11.021&partnerID=40&md5=cac64b66a83074737ff8e068509b5f6e},

doi = {10.1016/j.jhazmat.2015.11.021},

issn = {03043894},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Hazardous Materials},

volume = {305},

pages = {30-40},

publisher = {Elsevier B.V.},

abstract = {Graphene and its oxidized form-graphene oxide (GO) have become exceptionally popular in industry and medicine due to their unique properties. However, there are suspicions that GO can cause adverse effects. Therefore, comprehensive knowledge on its potential toxicity is essential.This research assesses the in vivo toxicity of pure and manganese ion contaminated GO, which were injected into the hemolymph of Acheta domesticus. The activity of catalase (CAT) and gluthiathione peroxidases (GSTPx) as well as heat shock protein (HSP 70) and total antioxidant capacity (TAC) levels were measured at consecutive time points-1. h, 24. h, 48. h and 72. h after injection.Neither pure GO nor GO contaminated with manganese were neutral to the organism. The results proved the intensification of oxidative stress after GO injection, which was confirmed by increased enzyme activity. The organism seems to cope with this stress, especially in the first 24. h after injection. In the following days, increasing HSP 70 levels were observed, which might suggest the synthesis of new proteins and the removal of old and damaged ones.With that in mind, the potential toxicity of the studied material, which could lead to serious and permanent damage to the organism, should still be taken into consideration. © 2015 Elsevier B.V.},

note = {33},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84949057523,

title = {The Comet assay in insects-Status, prospects and benefits for science},

author = { M. Augustyniak and M. Gladysz and M. Dziewięcka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949057523&doi=10.1016%2fj.mrrev.2015.09.001&partnerID=40&md5=048254bbf3587a3306b90a530981ee24},

doi = {10.1016/j.mrrev.2015.09.001},

issn = {13835742},

year  = {2016},

date = {2016-01-01},

journal = {Mutation Research - Reviews in Mutation Research},

volume = {767},

pages = {67-76},

publisher = {Elsevier B.V.},

abstract = {The Comet assay has been recently adapted to investigate DNA damage in insects. The first reports of its use in Drosophila melanogaster appeared in 2002. Since then, the interest in the application of the Comet assay to studies of insects has been rapidly increasing. Many authors see substantial potential in the use of the Comet assay in D. melanogaster for medical toxicology studies. This application could allow the testing of drugs and result in an understanding of the mechanisms of action of toxins, which could significantly influence the limited research that has been performed on vertebrates. The possible perspectives and benefits for science are considered in this review.In the last decade, the use of the Comet assay has been described in insects other than D. melanogaster. Specifically, methods to prepare a cell suspension from insect tissues, which is a difficult task, were analyzed and compared in detail. Furthermore, attention was paid to any differences and modifications in the research protocols, such as the buffer composition and electrophoresis conditions.Various scientific fields in addition to toxicological and ecotoxicological research were considered. We expect the Comet assay to be used in environmental risk assessments and to improve our understanding of many important phenomena of insect life, such as metamorphosis, molting, diapause and quiescence. The use of this method to study species that are of key importance to humans, such as pests and beneficial insects, appears to be highly probable and very promising. The use of the Comet assay for DNA stability testing in insects will most likely rapidly increase in the future. © 2015 Elsevier B.V.},

note = {48},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84923569075,

title = {DNA damage in grasshopper Chorthippus brunneus (Orthoptera) hatchlings following paraquat exposure},

author = { M. Augustyniak and T. Nocon and A. Kędziorski and P. Łaszczyca and T. Sawczyn and M. Tarnawska and A. Zawisza-Raszka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923569075&doi=10.1016%2fj.chemosphere.2014.12.069&partnerID=40&md5=f1d2caec2debb3c806d9441a50130558},

doi = {10.1016/j.chemosphere.2014.12.069},

issn = {00456535},

year  = {2015},

date = {2015-01-01},

journal = {Chemosphere},

volume = {125},

pages = {212-219},

publisher = {Elsevier Ltd},

abstract = {Comet assay was applied to study genotoxic damage induced by paraquat (PQ) in brain cells of Chorthippus brunneus (Insecta: Orthoptera) hatchlings. Percentage of the comet fluorescence in the tail (TDNA), length of the comet tail (TL) and Olive tail moment (OTM) were used for quantitative assessment of the DNA damage. Multiple regression analysis supplemented standard statistical elaboration of the results. Increasing PQ concentrations applied either directly to the brain cells suspension (10; 50; and 250. μM PQ final concentration - in vitro protocol) or indirectly (50; 250; and 1250. μM PQ final concentration - in vivo protocol) provoked significant increase of oxidative damage to DNA (higher median TDNA and OTM values). The damage increased with time of exposure (0; 5; 15; and 30. min) following in vitro application, but decreased in longer interval (3 vs 24. h) after in vivo administration of paraquat. On contrary, median TL values did not correlate with paraquat concentration irrespectively of the exposure protocol. Possible reason of this discrepancy in light of paraquat toxicity is discussed. © 2015 Elsevier Ltd.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84934873484,

title = {Ileal transposition in rats influenced glucose metabolism and HSP70 levels},

author = { D. Stygar and T. Sawczyn and B. Skrzep-Poloczek and I. Karcz-Socha and B. Doleżych and A. Zawisza-Raszka and M. Augustyniak and K. Żwirska-Korczala and W.K. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934873484&doi=10.1515%2fbiol-2015-0029&partnerID=40&md5=4231c33b26270314abfd79e73314f4dc},

doi = {10.1515/biol-2015-0029},

issn = {23915412},

year  = {2015},

date = {2015-01-01},

journal = {Open Life Sciences},

volume = {10},

number = {1},

pages = {278-284},

publisher = {De Gruyter Open Ltd},

abstract = {Objective: Ileal transposition procedure (IT), in combination with sleeve gastrectomy, is widely used to induce diabetes remission and to control related metabolic abnormalities. A transposition of a long segment of distal ileum in obese Zucker rats improved glucose tolerance 6 months after IT. The premise of our study was to to examine the long - term effects of ileum transposition on the liver glycolytic enzymes content in a euglycemic group of operated Zucker rats. Methods: Twenty male Zucker rats underwent either the transposition of 50% distal ileum or a sham surgery. Six months after surgery, liver tissue concentrations of glycogen synthase kinase alpha (GSK-3α), glucose 6-phosphatase (G6PC), glycogen phosphorylase (PYGM) and phosphofructokinase (PFK) and HSP70 were assessed by immunoenzymatic methods. Results: HSP70 values were significantly higher in the IT group compared to SHAM. G6PC liver concentrations in the IT group were almost 1.45-fold lower than in the SHAM operated rats. Statistical analyses (F-test) showed HSP70 levels were significantly related to caveolin-1and SHAM group. Conclusions: Lowered glycolytic enzyme concentrations assessed in the liver suggest positive effects on glucose metabolism in long-term observations. © 2015 Stygar Dominika et al. 2015.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84924420592,

title = {Elemental distribution in reproductive and neural organs of the epilachna nylanderi (Coleoptera: Coccinellidae), a phytophage of nickel hyperaccumulator Berkheya coddii (Asterales: Asteraceae) by micro-PIXE},

author = { J. Mesjasz-Przybyłowicz and E. Orłowska and M. Augustyniak and M. Nakonieczny and M. Tarnawska and W.J. Przybyłowicz and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924420592&doi=10.1093%2fjisesa%2fieu014&partnerID=40&md5=b9fa04f75525193486d5820a32e309b4},

doi = {10.1093/jisesa/ieu014},

issn = {15362442},

year  = {2014},

date = {2014-01-01},

journal = {Journal of Insect Science},

volume = {14},

publisher = {Library of the University of Arizona},

abstract = {The phenomenon of metal hyperaccumulation by plants is often explained by a pathogen or herbivore defense hypothesis. However, some insects feeding on metal hyperaccumulating plants are adapted to the high level of metals in plant tissues. Former studies on species that feed on the leaves of Berkheya coddii Roessler 1958 (Asteraceae), a nickel-hyperaccumulating plant, demonstrated several protective mechanisms involved in internal distribution, immobilization, and elimination of Ni from the midgut and Malpighian tubules. These species are mainly coleopterans, including the lady beetle, Epilachna nylanderi (Mulsant 1850) (Coleoptera: Coccinellidae), collected from the ultramafic ecosystem near Barberton in South Africa. By performing particle-induced X-ray emission microanalysis elemental microanalysis (PIXE), this study examined whether Ni may be harmful to internal body systems that decide on insect reactivity (central nervous system [CNS]), their reproduction, and the relationships between Ni and other micronutrients. Data on elemental distribution of nine selected elements in target organs of E. nylanderi were compared with the existing data for other insect species adapted to the excess of metals. Micro-PIXE maps of seven regions of the CNS showed Ni mainly in the neural connectives, while cerebral ganglia were better protected. Concentrations of other bivalent metals were lower than those of Ni. Testis, compared with other reproductive organs, showed low amounts of Ni. Zn was effectively regulated at physiological dietary levels. In insects exposed to excess dietary Zn, it was also accumulated in the reproductive organs. Comparison of E. nylanderii with other insects that ingest hyperaccumulating plants, especially chrysomelid Chrysolina clathrata (Clark) (Coleoptera: Chrysomelidae), showed lower protection of the CNS and reproductive organs. © The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84887612857,

title = {DNA damage in grasshoppers' larvae - Comet assay in environmental approach},

author = { M. Augustyniak and H. Orzechowska and A. Kędziorski and T. Sawczyn and B. Doleżych},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887612857&doi=10.1016%2fj.chemosphere.2013.10.033&partnerID=40&md5=5356747c3c954db75935f8623c8929d4},

doi = {10.1016/j.chemosphere.2013.10.033},

issn = {00456535},

year  = {2014},

date = {2014-01-01},

journal = {Chemosphere},

volume = {96},

pages = {180-187},

publisher = {Elsevier Ltd},

abstract = {The comet assay that provides a quantitative measure of the DNA-strand breaks may be used for assessing the 'genotoxic potential' of the environment. Young adults of Chorthippus brunneus (Orthoptera), collected at three sites in Southern Poland, differing in the level of pollution, particularly with heavy metals: Pilica (reference), Olkusz (moderately polluted) and Szopienice (heavily polluted) - were allowed to mate under laboratory conditions that were free from any pollution. Egg-pods were collected and, after diapause, brain cells from one-day old larvae were used for the comet assay. We compared the level of DNA damage in the larvae originating from these sites and also measured time-dependent DNA repair after single 10min. application of H2O2 (20μM final concentration).The DNA damage was relatively low in larval cells irrespectively of the site pollution their parents came from. However, measured comet parameters - tail DNA content (TDNA), tail length (TL), and olive tail moment (OTM) - were significantly higher in larvae originating from the Szopienice site than in those from the reference site. Incubation of cells with H2O2 resulted in significantly higher values of the comet parameters in the insects from all the study sites with the highest ones observed in the offspring of grasshoppers from Szopienice. Moreover, DNA repair, following the treatment, did not occur in the latter group. These data contribute to almost unexplored subject of genotoxic effects of environmental pollutants in insects. They are discussed in the light of the concept of adaptive strategies in energy allocation depending on the level of biotope pollution. © 2013 Elsevier Ltd.},

note = {29},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84862844501,

title = {Alteration of carbohydrates metabolism and midgut glucose absorption in Gromphadorhina portentosa after subchronic exposure to imidacloprid and fenitrothion},

author = { T. Sawczyn and B. Doleżych and M. Kłosok and M. Augustyniak and D. Stygar and R.J. Buldak and M. Kukla and K. Michalczyk and I. Karcz-Socha and K. Żwirska-Korczala},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862844501&doi=10.1080%2f10934529.2012.687181&partnerID=40&md5=d606fa43dbd2422f04afdf1ba97c188b},

doi = {10.1080/10934529.2012.687181},

issn = {10934529},

year  = {2012},

date = {2012-01-01},

journal = {Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering},

volume = {47},

number = {11},

pages = {1644-1651},

abstract = {This study was undertaken to test the hypothesis that following exposure to insecticides, changes take place in the metabolism of carbohydrates and absorption in the midgut of insects. TheMadagascar hissing cockroach (Gromphadorhina portentosa) was chosen for the experiment as a model organism, due to it being easy to breed and its relatively large alimentary tract, which was important when preparing the microperfusion midgut bioassay. In each group of cockroaches treated with imidacloprid and fenitrothion, absorption of glucose, expressed as the area under the curve (AUC), was elevated compared to the control group. Glucose in the hemolymph of the examined insects was present in a vestigial amount, often below the threshold of determination, so the determinable carbohydrate indices were: hemolymph trehalose concentration and fat body glycogen content. The level of trehalose found in the hemolymph of insects when exposed to fenitrothion, and irrespective of the level of concentration mixed into food, were significantly lower when comparing to the control samples. Imidacloprid acted analogically with one exception at the concentration of 10 mg·kg-1dry food where trehalose concentration did not differ from the control values. Coupling with fat body glycogen concentration was less visible and appeared only at the concentrations of 5 and 10 mg imidacloprid·kg-1 dry food. As described in this study changes in the sugar distribution and midgut glucose absorption indicate that insects cover the increased energy needs induced by insecticides; also at the gastrointestinal tract level. The result indicates that the midgut glucose absorption parameters could be considered as a non-specific biomarker of insecticide toxicity. Copyright © Taylor & Francis Group, LLC.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79955156773,

title = {Micro-PIXE studies of Ni-elimination strategies in representatives of two families of beetles feeding on Ni-hyperaccumulating plant Berkheya coddii},

author = { P. Migula and W.J. Przybyłowicz and M. Nakonieczny and M. Augustyniak and M. Tarnawska and J. Mesjasz-Przybyłowicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955156773&doi=10.1002%2fxrs.1310&partnerID=40&md5=61672de73a5cbfaa8e978a3de14d6f8f},

doi = {10.1002/xrs.1310},

issn = {00498246},

year  = {2011},

date = {2011-01-01},

journal = {X-Ray Spectrometry},

volume = {40},

number = {3},

pages = {194-197},

abstract = {Plants may use the ability to hyperaccumulate toxic metals in their defence against herbivores. Insects can counteract and feed on toxic plants. Among grazers of Ni-hyperaccumulating Berkheya coddii, some beetles tolerate Ni (Chrysolina clathrata; former name Chrysolina pardalina; Epilachna nylanderi). The adaptive mechanisms against Ni toxicity were studied in these species using micro-PIXE quantitative elemental mapping and by microscopy techniques. Distinct differences were found in mechanisms used for Ni-elimination in both species. Functional comparisons and quantification of data extracted from selected micro-areas in target organs responsible for metal circulation (digestive tract and Malpighian tubules) were made. Similar detoxification mechanism is used against metals through spheric granules, which in E. nylanderi are formed mainly in the midgut, while in C. clathrata in Malpighian tubules and midgut. Elemental maps showed positive correlations of Ni and Zn in Malpighian tubules of E. nylanderi without such effect in C. clathrata. Quantitative relations of K+ and Cl- indicate higher osmotic pressure increasing reabsorption of water necessary for Ni transportation to the intestine lumen and further rejection outside the body. © 2011 John Wiley & Sons, Ltd.},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79960965309,

title = {Oxidative stress in newly-hatched Chorthippus brunneus-the effects of zinc treatment during diapause, depending on the female's age and its origins},

author = { M. Augustyniak and A. Babczyńska and M.G. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960965309&doi=10.1016%2fj.cbpc.2011.05.004&partnerID=40&md5=95eee9ed4b905494321a08cbc6523ae5},

doi = {10.1016/j.cbpc.2011.05.004},

issn = {15320456},

year  = {2011},

date = {2011-01-01},

journal = {Comparative Biochemistry and Physiology - C Toxicology and Pharmacology},

volume = {154},

number = {3},

pages = {172-179},

publisher = {Elsevier Inc.},

abstract = {The responses of glutathione, glutathione S-transferases (GSTs), and catalase (CAT) were determined in 1-day-old larvae of Chorthippus brunneus Thunberg, 1815, a grasshopper exposed to zinc during diapause, from unpolluted (Pilica) or polluted (Olkusz; Szopienice) sites. The aim of the work was to search for differences among populations of the insects as a result of various multistress pressures in their habitats. The question of zinc toxicity in the context of energy allocation was also considered. Zinc caused a decrease in glutathione concentration in the body of zinc-treated larvae. Significant differences between control and zinc-treated groups were confirmed for young females' progeny from Pilica and Olkusz as well as old females' progeny from Olkusz. GSTs activity was generally not influenced by zinc. It is possible that GSTs were not the most important target of zinc action. On the contrary, the influence of zinc on CAT activity was found. The increase in CAT activity after zinc treatment was similar for all studied populations. An increase in CAT activity after zinc exposure seems to be the most universal reaction. CAT activity in zinc-treated grasshoppers may explain the mechanism of zinc toxicity based on reactive oxygen forms generation. © 2011 Elsevier Inc.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-67549092938,

title = {Hsp70 level in progeny of aging grasshoppers from variously polluted habitats and additionally exposed to zinc during diapause},

author = { M. Augustyniak and M. Tarnawska and A. Babczyńska and M.G. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67549092938&doi=10.1016%2fj.jinsphys.2009.04.009&partnerID=40&md5=c9468174a580b05f85fecf42df645691},

doi = {10.1016/j.jinsphys.2009.04.009},

issn = {00221910},

year  = {2009},

date = {2009-01-01},

journal = {Journal of Insect Physiology},

volume = {55},

number = {8},

pages = {735-741},

abstract = {The hsp70 level in the bodies of 1st instars of grasshoppers Chorthippus brunneus from unpolluted (Pilica) and polluted (Olkusz; Szopienice) sites and additionally exposed to various doses of zinc during diapause and embryonic development prior to hatching were measured by Western blotting. The main aim of our work was to assess the relationship between the age of female grasshoppers originating from variously polluted habitat and the hsp70 level in their progeny. Possible reasons for population variation in hsp70 levels were discussed. The hsp70 level in the offspring's body depended on the place of origin. The strongest expression of hsp70 was found in the bodies of larvae hatching from the eggs laid by young females from Pilica (reference site). In contrast, a low initial level of hsp70 in larvae from polluted sites, especially in young females' progeny, was observed. The application of zinc during diapause influenced the hsp70 level in grasshopper larvae; however, the direction of the changes depended on the insects' place of origin. In larvae from the reference site, and also (but to a lesser degree) from Olkusz, the increase in the hsp70 level after zinc treatment was most pronounced. Whereas in grasshoppers from Szopienice, zinc (in 100 μg g-1 dry weight of sand) did not change the hsp70 level, or (in 500 μg g-1 dry weight of sand) caused a reduction in hsp70. The differences may result from maternal effects; however, possible adaptation also cannot be excluded. To confirm this statement further studies are needed. © 2009 Elsevier Ltd. All rights reserved.},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-60049086660,

title = {Does the grasshopper Chorthippus brunneus adapt to metal polluted habitats? A study of glutathione-dependent enzymes in grasshopper nymphs},

author = { M. Augustyniak and A. Babczyńska and M.G. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-60049086660&doi=10.1111%2fj.1744-7917.2009.00251.x&partnerID=40&md5=16dfc6769ff7fbdbf35f9882aa04d166},

doi = {10.1111/j.1744-7917.2009.00251.x},

issn = {16729609},

year  = {2009},

date = {2009-01-01},

journal = {Insect Science},

volume = {16},

number = {1},

pages = {33-42},

abstract = {Glutathione contents and activity of glutathione-dependent enzymes in the body of insects inhabiting polluted areas depend on toxin kind, concentration and exposure time. Enzymatic response may be modified by gender, age, developmental stage and state of nutrition. Also, chronic exposure to metals in the environment may cause the selection of individuals resistant to some environmental toxins. To assess the degree of adaptation of Chorthippus brunneus to metal-polluted habitats, we measured glutathione contents and the activity of selected glutathione-dependent enzymes in the offspring of aging mothers which differed in time and intensity of exposure to metals in their habitats. We tested whether differences represent temporal shifts in tolerance range or were genetically preserved and inherited by future generations. We investigated insects from three populations. Two live in heavily metal-burdened areas, exposed to metals for 170 (Szopienice) or 50 years (Olkusz) and the third inhabits an unpolluted reference site (Pilica). The most important findings were age-by-site interactions for all biochemical analyses. Nymphs from Szopienice had lower glutathione contents and lower glutathione-dependent enzyme activity in comparison with nymphs from the reference site. This was especially distinct in nymphs hatched from eggs laid by young females. The offspring of aging females from Olkusz, in terms of glutathione contents and glutathione reductase activities, revealed similar patterns to those from the reference site. For the remaining parameters, enzyme activity patterns in nymphs from Olkusz were similar to those of nymphs from Szopienice. © Institute of Zoology, Chinese Academy of Sciences.},

note = {26},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-38149021340,

title = {Effects of zinc and female aging on nymphal life history in a grasshopper from polluted sites},

author = { M. Augustyniak and A. Babczyńska and M. Kozłowski and T. Sawczyn and M.G. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-38149021340&doi=10.1016%2fj.jinsphys.2007.08.002&partnerID=40&md5=a3b06cb34ffa434e19c4a09e20be051a},

doi = {10.1016/j.jinsphys.2007.08.002},

issn = {00221910},

year  = {2008},

date = {2008-01-01},

journal = {Journal of Insect Physiology},

volume = {54},

number = {1},

pages = {41-50},

abstract = {Insect reproduction is influenced by various factors, including food quality and quantity, temperature, population density and female age. Contamination, including heavy metals, may disturb reproductive processes. The aim of this work was to assess interactions between effects of aging in female Chorthippus brunneus and environmental pollution on their reproduction measured in number of laid eggs. We also compared basic developmental parameters (number of hatchlings; body mass; embryonic developmental rate) in grasshopper nymphs additionally exposed to zinc during diapause. Aging grasshoppers from heavily polluted areas (Olkusz and Szopienice) lay significantly fewer eggs than insects from the reference site (Pilica). Zinc application caused the decrease in hatching success and duration of embryogenesis in insects from each site. This suggests a cumulative effect of female age, pollutants and additional stressing factors. The intensity of this process differed between populations. In insects from the reference site, it was shown in a moderate degree. In insects from Szopienice, an additional stressor exerted a weaker effect than in insects from Pilica. In grasshoppers from Olkusz, we found the strongest decrease of hatching percentage and increase in duration of embryogenesis after zinc intoxication. This may indicate that the population from Olkusz exists at the limit of its energetic abilities. © 2007 Elsevier Ltd. All rights reserved.},

note = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-35548947013,

title = {Short-term effects of dimethoate on metabolic responses in Chrysolina pardalina (Chrysomelidae) feeding on Berkheya coddii (Asteraceae), a hyper-accumulator of nickel},

author = { M. Augustyniak and P. Migula and J. Mesjasz-Przybyłowicz and M. Tarnawska and M. Nakonieczny and A. Babczyńska and W.J. Przybyłowicz and M.G. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-35548947013&doi=10.1016%2fj.envpol.2007.01.018&partnerID=40&md5=144d40e423946ba636a4893b7c289520},

doi = {10.1016/j.envpol.2007.01.018},

issn = {02697491},

year  = {2007},

date = {2007-01-01},

journal = {Environmental Pollution},

volume = {150},

number = {2},

pages = {218-224},

abstract = {Berkheya coddii Roessler (Asteraceae) is a hyper-accumulator of nickel, which can be used in phytomining and phytoremediation. Chrysolina pardalina Fabricius (Chrysomelidae) is a phytophagous leaf beetle, which may be useful in controlling population levels of B. coddii after it has been introduced into a new habitat. The aim of this study was to investigate the response of C. pardalina to topical application of dimethoate. Data recorded included the activity of acetylcholinesterase (AChE), the concentration of glutathione (GSH), and the activity of selected enzymes connected with GSH metabolism. Assays were carried out several times during the first 24 h after exposure to dimethoate. At the dosages used in this study, dimethoate was not as toxic as expected. AChE activity was significantly decreased 14 and 24 h after application. GST activity was significantly decreased 24 h after application. GSTPx activity was significantly decreased 2, 14 and 24 h after application. GR activity was significantly increased 4 h after application. GSH concentration was significantly increased 24 h after application. Long-term exposure to high levels of nickel may have caused adaptive changes in the enzymes that enable C. pardalina to deal with other stressors, including organophosphate pesticides. © 2007 Elsevier Ltd. All rights reserved.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34249997029,

title = {Nickel toxicity in the hepatopancreas of an isopod Porcellio scaber (Oniscidea)},

author = { M. Tarnawska and P. Migula and W.J. Przybyłowicz and J. Mesjasz-Przybyłowicz and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34249997029&doi=10.1016%2fj.nimb.2007.02.082&partnerID=40&md5=04fc0ac3f07189d95963f1c89d2c4068},

doi = {10.1016/j.nimb.2007.02.082},

issn = {0168583X},

year  = {2007},

date = {2007-01-01},

journal = {Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms},

volume = {260},

number = {1},

pages = {213-217},

abstract = {This study is focused on recognizing how the functional role of hepatopancreas, the main metal storage organ in woodlice, is affected by the excess of nickel, a toxic element to soil invertebrates. Chronic Ni toxicity (24 weeks) was studied on four groups of woodlice kept on dry shredded maple leaves contaminated with Ni at average concentrations of 0.1 μg g-1 (control), 8.0 μg g-1 (Ni1), 75 μg g-1 (Ni2) and 270 μg g-1(Ni3) dry weight. Micro-PIXE mapping of elemental distribution in the hepatopancreas of the Porcellio scaber woodlice was used to study relations between nickel and other elements in individuals exposed to different metal concentrations in the diet. Data were processed using GeoPIXE II software. Transmission electron microscopy (TEM) was used to check the relations between ultrastructural changes in hepatopancreatic cells and nickel tissue burden. Elemental mapping showed a dose-related nickel bioaccumulation in the hepatopancreas at concentrations from 3 μg g-1 (uncontaminated control animals) to nearly 840 μg g-1 (Ni3). Generally, nickel was distributed uniformly in small aggregations. A combined evaluation of elemental maps and electronograms showed that aggregations of nickel in arbitrarily selected micro-areas in PIXE maps could be the granular structures observed in TEM electronograms. The mechanism of Ni sequestration in the hepatopancreas could be similar to this used for cadmium or lead. The sequences of ultrastructural changes, which follow the increased burdens of Ni in the hepatopancreatic cells, were: the increase of intracellular electron-dense granules, increase in the number of myelin-like structures, intensified mitochondrial swelling and appearance of concentrically arranged, rough endoplasmic reticulum. © 2007 Elsevier B.V. All rights reserved.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34249995164,

title = {Nickel toxicity in the hindgut of an isopod Porcellio scaber (Oniscidea)},

author = { M. Tarnawska and P. Migula and W.J. Przybyłowicz and J. Mesjasz-Przybyłowicz and M. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34249995164&doi=10.1016%2fj.nimb.2007.02.081&partnerID=40&md5=6a0ed8cee8ea54fa364eaa9dcf05b161},

doi = {10.1016/j.nimb.2007.02.081},

issn = {0168583X},

year  = {2007},

date = {2007-01-01},

journal = {Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms},

volume = {260},

number = {1},

pages = {222-226},

abstract = {Studies on toxic effects of nickel were conducted in a chronic experiment on four groups of woodlice Porcellio scaber kept for 24 weeks on dry maple leaves containing Ni 0.1 (control), 8 (Ni1), 75 (Ni2) and 270 (Ni3) μg g-1 dry weight. Micro-PIXE elemental mapping complemented by proton backscattering was used to study relations between Ni and other elements in the hindgut. Data processed with GeoPIXE II demonstrated the highest amount of Ni in the hindgut of (Ni2) woodlice followed by the highest levels of Ca, Mn and Fe. Inverse relationship was observed between hindgut concentration of Zn and the Ni levels in the diet. Transmission electron microscopy (TEM) was used for checking possible relations between the ultrastructure of the hindgut epithelial cells and nickel tissue burden. In animals from all the "nickel groups" a series of ultrastructural alterations (disturbances in apical and basal membrane labyrinth and presence of lysosome-like structures with electron-dense material in the cytoplasm) was identified when compared with control woodlice. Large mitochondria with distinctly visible electron-dense precipitations inside them were present in the cells of animals from groups (Ni1) and (Ni3). In addition, a disturbed internal mitochondrial membrane system was observed in the cells of the (Ni2) woodlice group. © 2007 Elsevier B.V. All rights reserved.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33751528790,

title = {Zinc-induced DNA damage and the distribution of metals in the brain of grasshoppers by the comet assay and micro-PIXE},

author = { M. Augustyniak and J. Kwaśniewska and W.J. Przybyłowicz and J. Mesjasz-Przybyłowicz and A. Babczyńska and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33751528790&doi=10.1016%2fj.cbpc.2006.09.003&partnerID=40&md5=f3ec30bff89e31320c5b4f2b3f1b39ac},

doi = {10.1016/j.cbpc.2006.09.003},

issn = {15320456},

year  = {2006},

date = {2006-01-01},

journal = {Comparative Biochemistry and Physiology - C Toxicology and Pharmacology},

volume = {144},

number = {3},

pages = {242-251},

publisher = {Elsevier Inc.},

abstract = {The distribution and concentration of selected elements by PIXE method and DNA damage using comet assay in brains of 1st instars of grasshoppers Chorthippus brunneus from unpolluted (Pilica) and polluted (Olkusz) site, additionally exposed to various doses of zinc during diapause or after hatching, were measured. We tried to assess the degree of possible pre-adaptation of the insects to heavy metals and evaluate the utility of these parameters in estimation of insect exposure to industrial pollutants. Additionally, the mechanism of zinc toxicity for grasshopper brains was discussed. We observed the correlation between experimental zinc dose, zinc contents in the brain and DNA damage in neuroblasts, but only in groups exposed to lower zinc concentration. For higher zinc concentration the amount of the metal in brain and DNA damage remained at the control level. Some site-related differences in DNA damage between grasshoppers from Pilica and Olkusz were observed during short-term exposure (after hatching). Significant increase in the calcium contents in the brain, proportional to zinc concentration in sand, was also observed, especially in the offsprings from Olkusz. The results may be the basis for further searching for molecular mechanisms of defense against heavy metals in insects living in polluted habitats. © 2006 Elsevier Inc. All rights reserved.},

note = {52},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-27744505338,

title = {Joint effects of dimethoate and heavy metals on metabolic responses in a grasshopper (Chorthippus brunneus) from a heavy metals pollution gradient},

author = { M. Augustyniak and A. Babczyńska and P. Migula and G. Wilczek and P. Łaszczyca and A. Kafel and M.G. Augustyniak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-27744505338&doi=10.1016%2fj.cbpc.2005.09.007&partnerID=40&md5=1761544f1ffb0849417b3d03dcae3174},

doi = {10.1016/j.cbpc.2005.09.007},

issn = {15320456},

year  = {2005},

date = {2005-01-01},

journal = {Comparative Biochemistry and Physiology - C Toxicology and Pharmacology},

volume = {141},

number = {4},

pages = {412-419},

publisher = {Elsevier Inc.},

abstract = {We studied how an exposure to an additional stressing factor-dimethoate, might affect detoxifying ability of grasshoppers collected at 5 meadow sites located along a heavy metal pollution gradient. Activities of esterases and enzymes linked with glutathione (GSH) metabolism were assayed 24 h after topical treatment with 0.32 μg dimethoate per insect. Inhibition of acetylcholinesterase (AChE) reaches nearly 50% of the value stated in untreated insects, without significant site-dependent differences. The pesticide also caused a significant decrease in activities of glutathione peroxidase (GPx) followed by a decrease in GSH levels in grasshoppers from all assayed groups, demonstrating high sensitivity of glutathione-dependent metabolism to the additional stressing factor. In the case of glutathione reductase (GR) and carboxylesterases (CarE) the fall of activity was shown especially in insects from less polluted meadows and the reference site. Glutathione reductase (GR) activity in individuals treated with dimethoate did not decrease only in insects from the most contaminated site I. This might suggest the trade-off mechanisms adapting grasshoppers to life in seriously polluted environments. © 2005 Elsevier Inc. All rights reserved.},

note = {33},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-4344637322,

title = {Relations between metals (Zn, Pb, Cd and Cu) and glutathione-dependent detoxifying enzymes in spiders from a heavy metal pollution gradient},

author = { G. Wilczek and A. Babczyńska and M. Augustyniak and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-4344637322&doi=10.1016%2fj.envpol.2004.05.011&partnerID=40&md5=5cf2272bd3dc7414893acc61eaa86648},

doi = {10.1016/j.envpol.2004.05.011},

issn = {02697491},

year  = {2004},

date = {2004-01-01},

journal = {Environmental Pollution},

volume = {132},

number = {3},

pages = {453-461},

abstract = {We studied the relations between glutathione-dependent detoxifying enzymes and heavy metal burdens in the web-building spider Agelena labyrinthica (Agelenidae) and the wolf spider Pardosa lugubris (Lycosidae) from five meadow sites along a heavy metal pollution gradient. We assayed the activity of glutathione-S-transferase (GST) and glutathione peroxidases (GPOX; GSTPx), and glutathione (GSH) levels in both sexes. Except for GSH vs Pb content, we found significant correlations between GPOX and GSTPx activity and metal concentrations in females of A. labyrinthica. The highest activity of these enzymes measured in the web-building spiders was found in the individuals from the most polluted sites. In P. lugubris males significant correlations were found between GST and Pb and Zn concentrations, and between GPOX and GSTPx and the concentration of Cu. GST activity was higher in males collected from less polluted areas. Thus, detoxifying strategies against pollutants seemed to be sex-dependent. Actively hunting spiders had higher metal concentrations, maintaining lower activity of detoxifying enzymes and a lower glutathione level. Glutathione-linked enzyme activity in spiders from polluted areas depends on hunting strategy and sex. © 2004 Elsevier Ltd. All rights reserved.},

note = {74},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-15444375342,

title = {Uptake of cadmium, lead, nickel and zinc from soil and water solutions by the nickel hyperaccumulator berkheya coddii},

author = { J. Mesjasz-Przybyłowicz and M. Nakonieczny and P. Migula and M. Augustyniak and M. Tarnawska and W.U. Reimold and C. Koeberl and W.J. Przybyłowicz and E. Głowacka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-15444375342&partnerID=40&md5=dc4ede8c4a0607a1f92bba5e4b45faf0},

issn = {00015296},

year  = {2004},

date = {2004-01-01},

journal = {Acta Biologica Cracoviensia Series Botanica},

volume = {46},

pages = {75-85},

abstract = {Berkheya coddii Roessler (Asteraceae), an endemic herbaceous and perennial nickel-hyperaccumulating plant growing on Ni-enriched ultramafic soils in South Africa, is perceived as a promising species for phytoremediation and phytomining due to its large biomass production and high Ni content. Total concentrations of a number of elements in mature leaves, soil and related bedrock were obtained. The average Ni concentration in leaves was 18,000 μg·g -1 dry mass, whereas in soil and bedrock the total amount of Ni was 1,300 μg·g-1 and 1,500 μg·g-1, respectively. Exceptionally high average Ni concentrations (55;000 ± 15;000 μg·g-1; n = 6) were found in B. coddii leaves from Songimvelo Game Reserve, including the highest-ever reported concentration of Ni in leaves (76;100 μg·g-1 - maximum value in a single sample). Young plants grown in pots with ultramafic soil accumulated small quantities of Cd, Pb or Zn, but the concentrations of these elements increased after the addition of metal solutions to the soil. Excised shoots immersed in concentrated solutions of Cd, Ni, Pb or Zn accumulated large amounts of these metals in the leaves. © Polish Academy of Sciences, Cracow 2004.},

note = {74},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-10044240191,

title = {Antioxidative defence enzymes in beetles from a metal pollution gradient},

author = { P. Migula and P. Łaszczyca and M. Augustyniak and G. Wilczek and K. Rozpędek and A. Kafel and M. Wołoszyn},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-10044240191&partnerID=40&md5=a309d39f85323f5fcc87cca33f05e9d3},

issn = {13356380},

year  = {2004},

date = {2004-01-01},

journal = {Biologia - Section Zoology},

volume = {59},

number = {5},

pages = {645-654},

abstract = {Oxidative stress in insects may result from an imbalance of oxidants and antioxidants under a significant impact of metals. We studied variation in antioxidant enzyme activity in four species of beetles representing carnivores, carrion eaters, omnivores and phytophages in response to heavy metal pollution. Insects were collected at five forest sites along a gradient of heavy metal pollution in southern Poland. Assayed activity of superoxide dismutase (SOD), glutathione reductase (GR), glutathione S-transferase (GST), Se-dependent (GPOX) and Se-independent (GSTP) glutathione peroxidases and catalase (CAT) correlated with metal pollution levels and body concentrations of metals (Pb; Zn; Cu; Cd). Antioxidative enzyme activity patterns were species-dependent and were correlated with the levels of metal pollution or the body loads of metals. Correlations were predominantly positive in the case of Pb and Cd, and both positive and negative in the case of Zn. The largest difference between species was in the GST activity of the carnivorous Pterostichus oblongopunctatus and the phytophagous Phyllobius betulae. Activity of GSH-dependent peroxidases and GR was low in most of the species studied. Beetles from metal-contaminated sites showed higher within-species variance of enzyme activity, probably due to their higher polymorphism of antioxidative enzymes.},

note = {51},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-2942670066,

title = {Profiles of enzymatic activity in earthworms from zinc, lead and cadmium polluted areas near Olkusz (Poland)},

author = { P. Łaszczyca and M. Augustyniak and A. Babczyńska and K. Bednarska and A. Kafel and P. Migula and G. Wilczek and I. Witas},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2942670066&doi=10.1016%2fj.envint.2004.02.006&partnerID=40&md5=fb1bf084ee1cffc308a2059a62a4a95c},

doi = {10.1016/j.envint.2004.02.006},

issn = {01604120},

year  = {2004},

date = {2004-01-01},

journal = {Environment International},

volume = {30},

number = {7},

pages = {901-910},

publisher = {Elsevier Ltd},

abstract = {The aim of the study was to determine whether there are signs of adaptation of soil fauna to a gradient of heavy metal contamination. Earthworms Aporrectodea caliginosa, Lumbricus terrestris and Eisenia fetida were collected during the spring and summer of 2000 and 2001 from meadow sites situated between 2 and 32 km from the Bukowno-Olkusz complex of zinc-lead ore mines and smelters. The heavy metal content in the soil near smelters reaches 10,500 mg/kg (d.w.) for Zn, 2600 mg/kg for Pb and 81.9 mg/kg for Cd. The sites differ with respect to species composition of earthworm community, with A. caliginosa being dominant. Complete data was obtained only for A. caliginosa, since other species were not abundant at all investigated sites during the whole period of investigation. The body burdens of Zn, Pb, Cd and Cu in A. caliginosa reached 1500, 100, 220 and 10 μg/g, respectively, in the vicinity of the smelter (2-4 km), and decreased to 400, 2, 36 and 6 μg/g at the most distant site (32 km). Cadmium and lead content was significantly elevated in the whole body of L. terrestris collected at the site 2.5 km distant from the smelters when compared to more distant sites, while in E. fetida only the body burden of cadmium was elevated at the nearest site compared to the next site of transect. Activities of glutathione peroxidase (GPX; EC 1.11.1.9) against hydrogen peroxide (H2O2) or cumene hydroperoxide (cumOOH), glutathione reductase (GR; EC 1.6.4.2), glutathione S-transferase (GST; EC 2.5.1.18) and catalase (CAT; EC 1.11.1.6) were assayed in postmitochondrial supernatant obtained from whole body homogenates. Seasonal and annual variations of enzyme activity were reflected by higher GPX activity in the late summer of 2001 in comparison with the spring and summer of 2000. This may reflect severe drought in the spring and summer of 2000. The activity of both GPX isozymes, GR and GST in A. caliginosa and L. terrestris increased with increasing distance from the smelters and reached maximum at sites III and IV (4 and 8 km from the smelters; respectively) and then it decreased in the animals from site V (32 km). These may be the effects of antagonism between the enzyme inducing and enzyme inhibiting action of smelter emissions, a phenomenon known as a hormetic effect. It is postulated here that this effect is of diagnostic value for metal pollution biomonitoring. © 2004 Elsevier Ltd. All rights reserved.},

note = {113},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0037340581,

title = {Activity of glutathione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations},

author = { A. Kafel and K. Bednarska and M. Augustyniak and I. Witas and E. Szulińska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037340581&doi=10.1016%2fS0160-4120%2802%2900111-3&partnerID=40&md5=8082a67a14755b7f7ed59ab4571f1c33},

doi = {10.1016/S0160-4120(02)00111-3},

issn = {01604120},

year  = {2003},

date = {2003-01-01},

journal = {Environment International},

volume = {28},

number = {8},

pages = {683-686},

publisher = {Elsevier Ltd},

abstract = {The aim of our study was to establish changes in activity of important in detoxification enzyme - glutathione S-transferase (GST): in alimentary tract, fat body and Malpighian tubules of Spodoptera exigua larvae being under cadmium and zinc exposure through the first as well as the second generation. There was registered enhancement of the enzyme activity in the fat body and the Malpighian tubules caused by zinc as well as its decrease in the Malpighian tubules under cadmium action. Amounts of metals in the alimentary tract were either several times higher than in the diet ingested by larvae or than in the fat body. Metal concentration in the fat body correlated with the level of the enzyme activity (positive correlation for zinc and negative for cadmium). The effect of metal action differentiated dependently on time exposition. © 2002 Elsevier Science Ltd. All rights reserved.},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77957098012,

title = {Chapter 16 Body burden with metals and detoxifying abilities of the grasshopper - Chorthippus brunneus (Thunberg) from industrially polluted areas},

author = { M. Augustyniak and P. Migula},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957098012&doi=10.1016%2fS0927-5215%2800%2980019-3&partnerID=40&md5=015be62756d3a0379459eab349c7d1cc},

doi = {10.1016/S0927-5215(00)80019-3},

issn = {09275215},

year  = {2000},

date = {2000-01-01},

journal = {Trace Metals in the Environment},

volume = {4},

number = {C},

pages = {423-454},

abstract = {Relations between detoxifying enzymes - glutathione peroxidase (GPOX), glutathione S-transferase (GST), catalase (CAT), carboxylesterases (CarE) and exposure to cadmium of Chorthippus brunneus living in areas under different pollution impact were studied. Activity patterns of GST and CAT were cadmium dependent. Insects from a population living in a weakly polluted site exposed to cadmium showed lower GST and CAT activity in the midgut and gonads but GPOX and CarE was higher and positively correlated with the level of cadmium in consumed grass and in their tissues. Effects of cadmium were more pronounced in females. Inhibitory effects of cadmium on GST and CAT activity in grasshoppers from a less polluted site was compensated by the GPOX with a high H2O2 affinity. Insects from heavily polluted site expressed higher involvement of catalase, suggesting their better detoxifying abilities. CarE showed higher activity rates with increased pollution gradient in insects from five different populations and with increased exposure to cadmium in laboratory experiments. © 2000 Elsevier B.V. All rights reserved.},

note = {56},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0034483816,

title = {Heavy metals, resting metabolism rates and breeding parameters in two populations of black gull Larus ridibundus from the insdustrially polluted areas of Upper Silesia, Poland [Skazenie metalami ciezkimi, metabolizm tlenowy i parametry legowe dwoch populacji legowych mewy smieszki na obszarach zanieczy szczonych przez przemysl (Górny Ślask)]},

author = { P. Migula and M. Augustyniak and A. Szymczyk and K. Kowalczyk},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034483816&doi=10.3161%2f068.035.0209&partnerID=40&md5=85910110c085f18306bda6b29b63b780},

doi = {10.3161/068.035.0209},

issn = {00016454},

year  = {2000},

date = {2000-01-01},

journal = {Acta Ornithologica},

volume = {35},

number = {2},

pages = {159-172},

publisher = {Polish Academy of Sciences},

abstract = {Black-headed Gulls breeding in the central part of the Upper Silesian Industrial Region (Katowice-Szopienice) in Southern Poland were compared with colony from less polluted area (Świerklaniec) situated 23 km away. Heavy metals: Cd, Pb, Zn and Cu - were determined in various organs of nestlings, fledglings and mature birds as well as egg yolks. Resting metabolic rates, hatching success and eggshell thickness were used as biomarkers of environmental exposure to industrial pollutants. The clutch size (2.97 versus 3.61) and hatching success (81.5% versus 87%) were lower in the colony from the more polluted site. The relatively high metal contents in the yolks indicate that off spring are only partially protected from toxic compounds. Gulls were able to regulate body contents of essential metals Zn and Cu, but Pb and Cd accumulated rapidly in the liver, kidneys and lungs of growing birds. Cd also accumulated in the ovaries at the similar levels as in the kidneys. Cd and Pb may have affected earlier stages of development when parents were foraged in a close vicinity of the heavily polluted area but once the young gulls had reached maturity they then foraged in distant areas. During this period they were exposed to pollutants in the same way as the gulls from the less polluted site. There was no growth impairment identified in gulls from the more polluted area, and their resting metabolism (RMR) calculated per unit of body weight was lower, indicating that energetic costs for detoxification were not as high.},

note = {13},

keywords = {},

pubstate = {published},

tppubtype = {article}

}