@article{2-s2.0-85194103059,

title = {DNA methylation analysis of floral parts revealed dynamic changes during the development of homostylous Fagopyrum tataricum and heterostylous F. esculentum flowers},

author = { K. Sala and A. Tomasiak and K. Nowak and A. Piński and A. Betekhtin},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85194103059&doi=10.1186%2fs12870-024-05162-w&partnerID=40&md5=42bf1d5a9f3df72050790bf730e2066d},

doi = {10.1186/s12870-024-05162-w},

issn = {14712229},

year  = {2024},

date = {2024-01-01},

journal = {BMC Plant Biology},

volume = {24},

number = {1},

publisher = {BioMed Central Ltd},

abstract = {Background: Proper flower development is essential for plant reproduction, a crucial aspect of the plant life cycle. This process involves precisely coordinating transcription factors, enzymes, and epigenetic modifications. DNA methylation, a ubiquitous and heritable epigenetic mechanism, is pivotal in regulating gene expression and shaping chromatin structure. Fagopyrum esculentum demonstrates anti-hypertensive, anti-diabetic, anti-inflammatory, cardio-protective, hepato-protective, and neuroprotective properties. However, the heteromorphic heterostyly observed in F. esculentum poses a significant challenge in breeding efforts. F. tataricum has better resistance to high altitudes and harsh weather conditions such as drought, frost, UV-B radiation damage, and pests. Moreover, F. tataricum contains significantly higher levels of rutin and other phenolics, more flavonoids, and a balanced amino acid profile compared to common buckwheat, being recognised as functional food, rendering it an excellent candidate for functional food applications. Results: This study aimed to compare the DNA methylation profiles between the Pin and Thrum flower components of F. esculentum, with those of self-fertile species of F. tataricum, to understand the potential role of this epigenetic mechanism in Fagopyrum floral development. Notably, F. tataricum flowers are smaller than those of F. esculentum (Pin and Thrum morphs). The decline in DNA methylation levels in the developed open flower components, such as petals, stigmas and ovules, was consistent across both species, except for the ovule in the Thrum morph. Conversely, Pin and Tartary ovules exhibited a minor decrease in DNA methylation levels. The highest DNA methylation level was observed in Pin stigma from closed flowers, and the most significant decrease was in Pin stigma from open flowers. In opposition, the nectaries of open flowers exhibited higher levels of DNA methylation than those of closed flowers. The decrease in DNA methylation might correspond with the downregulation of genes encoding methyltransferases. Conclusions: Reduced overall DNA methylation and the expression of genes associated with these epigenetic markers in fully opened flowers of both species may indicate that demethylation is necessary to activate the expression of genes involved in floral development. © The Author(s) 2024.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85190591678,

title = {Reconstruction pattern of the cell wall in Fagopyrum protoplast-derived hybrid cells},

author = { K. Sala and A. Milewska-Hendel and R. Pérez-Pérez and E. Grzebelus and A. Betekhtin},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85190591678&doi=10.1007%2fs11240-024-02740-6&partnerID=40&md5=4f87694909e5b5e6703682a32ea7f03a},

doi = {10.1007/s11240-024-02740-6},

issn = {01676857},

year  = {2024},

date = {2024-01-01},

journal = {Plant Cell, Tissue and Organ Culture},

volume = {157},

number = {2},

publisher = {Springer Science and Business Media B.V.},

abstract = {The cell wall rebuilding is one of the first stage of protoplast development that enables further mitotic divisions and differentiation. Therefore, this work focuses on the comparison of the cell wall regeneration in the parental protoplasts of Fagopyrum tataricum, F. esculentum and the F. tataricum (+) F. esculentum hybrids, which are promising materials in terms of future breeding and research programmes. It is worth emphasizing that the preparation of buckwheat hybrids using electrofusion was described for the first time. The results indicate that cell wall rebuilding exhibited a common mechanism for parent protoplasts and the heterokaryon as all analysed cell wall components recognising arabinogalactan proteins (JIM13; JIM16), extensin (JIM20), xyloglucan (LM25) and pectins (LM20; LM5; LM6) were detected during the process of wall regeneration. However, there were certainly differences in the spatio-temporal appearance or disappearance of individual epitopes during the 72 h of the cell culture, which have been discussed in the paper. © The Author(s) 2024.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85164024170,

title = {The Impact of Gold Nanoparticles on Somatic Embryogenesis Using the Example of Arabidopsis thaliana},

author = { K. Godel-Jędrychowska and A. Milewska-Hendel and K. Sala and R. Baranski and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85164024170&doi=10.3390%2fijms241210356&partnerID=40&md5=e32d3618b3f386a03753326e24a779bc},

doi = {10.3390/ijms241210356},

issn = {16616596},

year  = {2023},

date = {2023-01-01},

journal = {International Journal of Molecular Sciences},

volume = {24},

number = {12},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {Although the influence of nanoparticles (NPs) on developmental processes is better understood, little is known about their impact on somatic embryogenesis (SE). This process involves changes in the direction of cell differentiation. Thus, studying the effect of NPs on SE is essential to reveal their impact on cell fate. This study aimed to examine the influence of gold nanoparticles (Au NPs) with different surface charges on the SE of 35S:BBM Arabidopsis thaliana, with particular emphasis on the spatiotemporal localization of pectic arabinogalactan proteins (AGPs) and extensin epitopes in cells changing the direction of their differentiation. The results show that under the influence of nanoparticles, the explant cells of 35S:BBM Arabidopsis thaliana seedling origin did not enter the path of SE. Bulges and the formation of organ-like structures were observed in these explants, in contrast to the control, where somatic embryos developed. Additionally, spatiotemporal changes in the chemical composition of the cell walls during the culture were observed. Under the influence of Au NPs, the following effects were observed: (1) explant cells did not enter the SE pathway, (2) the impacts of Au NPs with different surface charges on the explants were variable, and (3) the compositions of the analyzed pectic AGPs and extensin epitopes were diverse in the cells with different developmental programs: SE (control) and non-SE (treated with Au NPs). © 2023 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85115042302,

title = {Gold nanoparticles‐induced modifications in cell wall composition in barley roots},

author = { A. Milewska-Hendel and K. Sala and W. Gepfert and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115042302&doi=10.3390%2fcells10081965&partnerID=40&md5=22a9870ffeb8d79b61ecfc26ec5c8c5d},

doi = {10.3390/cells10081965},

issn = {20734409},

year  = {2021},

date = {2021-01-01},

journal = {Cells},

volume = {10},

number = {8},

publisher = {MDPI},

abstract = {The increased use of nanoparticles (NP) in different industries inevitably results in their release into the environment. In such conditions, plants come into direct contact with NP. Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient. Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP). The analyses were performed using the immunohistological method and fluorescence microscopy. The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85108635869,

title = {Nanoparticles—plant interaction: What we know, where we are?},

author = { E.U. Kurczyńska and K. Godel-Jędrychowska and K. Sala and A. Milewska-Hendel},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108635869&doi=10.3390%2fapp11125473&partnerID=40&md5=5739d81152baef30b3dc2ed0c4b3f0f7},

doi = {10.3390/app11125473},

issn = {20763417},

year  = {2021},

date = {2021-01-01},

journal = {Applied Sciences (Switzerland)},

volume = {11},

number = {12},

publisher = {MDPI AG},

abstract = {In recent years; the interaction of nanoparticles (NPs) with plants has been intensively studied. Therefore, more and more aspects related to both the positive and negative impact of NP on plants are well described. This article focuses on two aspects of NP interaction with plants. The first is a summary of the current knowledge on NP migration through the roots into the plant body, in particular, the role of the cell wall. The second aspect summarizes the current knowledge of the participation of the symplast, including the plasmodesmata (PD), in the movement of NP within the plant body. We highlight the gaps in our knowledge of the plant–NP interactions; paying attention to the need for future studies to explain the mechanisms that regulate the composition of the cell wall and the functioning of the PD under the influence of NP. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85108065626,

title = {Inhibition of carotenoid biosynthesis by crispr/cas9triggers cell wall remodelling in carrot},

author = { T. Oleszkiewicz and M. Klimek-Chodacka and M. Kruczek and K. Godel-Jędrychowska and K. Sala and A. Milewska-Hendel and M. Zubko and E.U. Kurczyńska and Y. Qi and R. Baranski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108065626&doi=10.3390%2fijms22126516&partnerID=40&md5=08bdce802b200c7f39ad88ac5c476848},

doi = {10.3390/ijms22126516},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {12},

publisher = {MDPI},

abstract = {Recent data indicate that modifications to carotenoid biosynthesis pathway in plants alter the expression of genes affecting chemical composition of the cell wall. Phytoene synthase (PSY) is a rate limiting factor of carotenoid biosynthesis and it may exhibit species-specific and or-gan-specific roles determined by the presence of psy paralogous genes, the importance of which often remains unrevealed. Thus, the aim of this work was to elaborate the roles of two psy paralogs in a model system and to reveal biochemical changes in the cell wall of psy knockout mutants. For this purpose, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas9) proteins (CRISPR/Cas9) vectors were introduced to carotenoid-rich carrot (Daucus carota) callus cells in order to induce mutations in the psy1 and psy2 genes. Gene sequencing, expression analysis, and carotenoid content analysis revealed that the psy2 gene is critical for carotenoid bio-synthesis in this model and its knockout blocks carotenogenesis. The psy2 knockout also decreased the expression of the psy1 paralog. Immunohistochemical staining of the psy2 mutant cells showed altered composition of arabinogalactan proteins, pectins, and extensins in the mutant cell walls. In particular, low-methylesterified pectins were abundantly present in the cell walls of carote-noid-rich callus in contrast to the carotenoid-free psy2 mutant. Transmission electron microscopy revealed altered plastid transition to amyloplasts instead of chromoplasts. The results demonstrate for the first time that the inhibited biosynthesis of carotenoids triggers the cell wall remodelling. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85082955768,

title = {Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta},

author = { M. Popielarska-Konieczna and K. Sala and M. Abdullah and M. Tuleja and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082955768&doi=10.1007%2fs00299-020-02530-2&partnerID=40&md5=731be893a36b20b90eaa9a5eabc144a5},

doi = {10.1007/s00299-020-02530-2},

issn = {07217714},

year  = {2020},

date = {2020-01-01},

journal = {Plant Cell Reports},

volume = {39},

number = {6},

pages = {779-798},

publisher = {Springer},

abstract = {Key message: Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit. Abstract: The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue. In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta. The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway. The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli. The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures. The non-organogenic tissue was composed of loosely attached cells, which were connected via a net-like structure. The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19; LM20; JIM11; JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus. Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25; heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5; LM6; LM19; JIM11; JIM12; JIM20; JIM8 and JIM16 epitopes). © 2020, The Author(s).},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85066960172,

title = {Hydroxyproline-rich glycoproteins as markers of temperature stress in the leaves of brachypodium distachyon},

author = { A. Piński and A. Betekhtin and K. Sala and K. Godel-Jędrychowska and E.U. Kurczyńska and R. Hasterok},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066960172&doi=10.3390%2fijms20102571&partnerID=40&md5=83367841b3aa36bbcbe19816364135ad},

doi = {10.3390/ijms20102571},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {10},

publisher = {MDPI AG},

abstract = {Plants frequently encounter diverse abiotic stresses, one of which is environmental thermal stress. To cope with these stresses, plants have developed a range of mechanisms, including altering the cell wall architecture, which is facilitated by the arabinogalactan proteins (AGP) and extensins (EXT). In order to characterise the localisation of the epitopes of the AGP and EXT, which are induced by the stress connected with a low (4◦ C) or a high (40◦ C) temperature, in the leaves of Brachypodium distachyon, we performed immunohistochemical analyses using the antibodies that bind to selected AGP (JIM8; JIM13; JIM16; LM2 and MAC207), pectin/AGP (LM6) as well as EXT (JIM11; JIM12 and JIM20). The analyses of the epitopes of the AGP indicated their presence in the phloem and in the inner bundle sheath (JIM8; JIM13; JIM16 and LM2). The JIM16 epitope was less abundant in the leaves from the low or high temperature compared to the control leaves. The LM2 epitope was more abundant in the leaves that had been subjected to the high temperatures. In the case of JIM13 and MAC207, no changes were observed at the different temperatures. The epitopes of the EXT were primarily observed in the mesophyll and xylem cells of the major vascular bundle (JIM11; JIM12 and JIM20) and no correlation was observed between the presence of the epitopes and the temperature stress. We also analysed changes in the level of transcript accumulation of some of the genes encoding EXT, EXT-like receptor kinases and AGP in the response to the temperature stress. In both cases, although we observed the upregulation of the genes encoding AGP in stressed plants, the changes were more pronounced at the high temperature. Similar changes were observed in the expression profiles of the EXT and EXT-like receptor kinase genes. Our findings may be relevant for genetic engineering of plants with increased resistance to the temperature stress. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85064462014,

title = {Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union},

author = { K. Sala and J. Karcz and A. Rypień and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064462014&doi=10.1186%2fs12870-019-1748-4&partnerID=40&md5=b62468f34d858a8e2068f5b565af88af},

doi = {10.1186/s12870-019-1748-4},

issn = {14712229},

year  = {2019},

date = {2019-01-01},

journal = {BMC Plant Biology},

volume = {19},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: Grafting is a technique widely used in horticulture. The processes involved in grafting are diverse, and the technique is commonly employed in studies focusing on the mechanisms that regulate cell differentiation or response of plants to abiotic stress. Information on the changes in the composition of the cell wall that occur during the grafting process is scarce. Therefore, this study was carried out for analyzing the composition of the cell wall using Arabidopsis hypocotyls as an example. During the study, the formation of a layer that covers the surface of the graft union was observed. So, this study also aimed to describe the histological and cellular changes that accompany autografting of Arabidopsis hypocotyls and to perform preliminary chemical and structural analyses of extracellular material that seals the graft union. Results: During grafting, polyphenolic and lipid compounds were detected, along with extracellular deposition of carbohydrate/protein material. The spatiotemporal changes observed in the structure of the extracellular material included the formation of a fibrillar network, polymerization of the fibrillar network into a membranous layer, and the presence of bead-like structures on the surface of cells in established graft union. These bead-like structures appeared either "closed" or "open". Only three cell wall epitopes, namely: LM19 (un/low-methyl-esterified homogalacturonan), JIM11, and JIM20 (extensins), were detected abundantly on the cut surfaces that made the adhesion plane, as well as in the structure that covered the graft union and in the bead-like structures, during the subsequent stages of regeneration. Conclusions: To the best of our knowledge, this is the first report on the composition and structure of the extracellular material that gets deposited on the surface of graft union during Arabidopsis grafting. The results showed that unmethyl-esterified homogalacturonan and extensins are together involved in the adhesion of scion and stock, as well as taking part in sealing the graft union. The extracellular material is of importance not only due to the potential pectin-extensin interaction but also due to its origin. The findings presented here implicate a need for studies with biochemical approach for a detailed analysis of the composition and structure of the extracellular material. © 2019 The Author(s).},

note = {10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84966421090,

title = {Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae)},

author = { R. Gawecki and K. Sala and E.U. Kurczyńska and P. Świątek and B.J. Płachno},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966421090&doi=10.1007%2fs00709-016-0980-0&partnerID=40&md5=5294556454d52366eb2f810219227c05},

doi = {10.1007/s00709-016-0980-0},

issn = {0033183X},

year  = {2017},

date = {2017-01-01},

journal = {Protoplasma},

volume = {254},

number = {2},

pages = {657-668},

publisher = {Springer-Verlag Wien},

abstract = {In apomictic Taraxacum species, the development of both the embryo and the endosperm does not require double fertilisation. However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions. The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum. The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum. © 2016, The Author(s).},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85013892983,

title = {Quantitative and qualitative characteristics of cell wall components and prenyl lipids in the leaves of Tilia x euchlora trees growing under salt stress},

author = { A. Milewska-Hendel and A.H. Baczewska and K. Sala and W. Dmuchowski and P. Bragoszewska and D. Gozdowski and A. Jozwiak and T. Chojnacki and E. Swiezewska and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013892983&doi=10.1371%2fjournal.pone.0172682&partnerID=40&md5=9273a1f5b327a5d5316ca4927ba2751f},

doi = {10.1371/journal.pone.0172682},

issn = {19326203},

year  = {2017},

date = {2017-01-01},

journal = {PLoS ONE},

volume = {12},

number = {2},

publisher = {Public Library of Science},

abstract = {The study was focused on assessing the presence of arabinogalactan proteins (AGPs) and pectins within the cell walls as well as prenyl lipids, sodium and chlorine content in leaves of Tilia x euchlora trees. The leaves that were analyzed were collected from trees with and without signs of damage that were all growing in the same salt stress conditions. The reason for undertaking these investigations was the observations over many years that indicated that there are trees that present a healthy appearance and trees that have visible symptoms of decay in the same habitat. Leaf samples were collected from trees growing in the median strip between roadways that have been intensively salted during the winter season for many years. The sodium content was determined using atomic spectrophotometry, chloride using potentiometric titration and poly-isoprenoids using HPLC/UV. AGPs and pectins were determined using immunohistochemistry methods. The immunohistochemical analysis showed that rhamnogalacturonans I (RG-I) and homogalacturonans were differentially distributed in leaves from healthy trees in contrast to leaves from injured trees. In the case of AGPs, the most visible difference was the presence of the JIM16 epitope. Chemical analyses of sodium and chloride showed that in the leaves from injured trees, the level of these ions was higher than in the leaves from healthy trees. Based on chromatographic analysis, four polyisoprenoid alcohols were identified in the leaves of T. x euchlora. The levels of these lipids were higher in the leaves from healthy trees. The results suggest that the differences that were detected in the apoplast and symplasm may be part of the defensive strategy of T. x euchlora trees to salt stress, which rely on changes in the chemical composition of the cell wall with respect to the pectic and AGP epitopes and an increased synthesis of prenyl lipids. © 2017 Milewska-Hendel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85010756219,

title = {Distribution of some pectic and arabinogalactan protein epitopes during Solanum lycopersicum (L.) adventitious root development},

author = { K. Sala and K. Malarz and P.W. Barlow and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010756219&doi=10.1186%2fs12870-016-0949-3&partnerID=40&md5=36843d57a835eaa1f1f38157bc92d206},

doi = {10.1186/s12870-016-0949-3},

issn = {14712229},

year  = {2017},

date = {2017-01-01},

journal = {BMC Plant Biology},

volume = {17},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: The adventitious roots (AR) of plants share the same function as primary and lateral roots (LR), although their development is mainly an adaptive reaction to stress conditions. Regeneration of grafted plants is often accompanied by AR formation thus making the grafting technique a good model for studying AR initiation and development and their means of emergence. Pectins and arabinogalactan proteins (AGP) are helpful markers of particular cellular events, such as programmed cell death (PCD), elongation, proliferation or other differentiation events that accompany AR development. However, little is known about the distribution of pectins and AGPs during AR ontogeny, either in the primordium or stem tissues from which AR arise or their correspondence with these events during LR formation. Results: AR were developed from different stem tissues such as parenchyma, xylem rays and the cambium, depending on the stem age and treatment (grafting versus cutting) of the parental tissue. Immunochemical analysis of the presence of pectic (LM8; LM19; LM20) and AGP (JIM8; JIM13; JIM16) epitopes in AR and AR-associated tissues showed differential, tissue-specific distributions of these epitopes. Two pectic epitopes (LM19; LM20) were developmentally regulated and the occurrence of the LM8 xylogalacturonan epitope in the root cap of the AR differed from other species described so far. AGP epitopes were abundantly present in the cytoplasmic compartments (mainly the tonoplast) and were correlated with the degree of cell vacuolisation. JIM8 and JIM13 epitopes were detected in the more advanced stages of primordium development, whereas the JIM16 epitope was present from the earliest division events of the initial AR cells. The comparison between AR and LR showed quantitative (AGP;) and qualitative (pectins) differences. Conclusion: The chemical compositions of adventitious and lateral root cells show differences that correlate with the different origins of these cells. In AR, developmental changes in the distribution of pectins and AGP suggest the turnover of wall compounds. Our data extend the knowledge about the distribution of pectin and AGP during non-embryogenic root development in a species that is important from an agronomic point of view. © 2017 The Author(s).},

note = {21},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021764617,

title = {Morpho-histological analysis of tomato (Solanum lycopersicum L.) plants after treatment with juglone},

author = { A. Milewska-Hendel and M. Polak and K. Sala and P. Zieleźnik-Rusinowska and R. Gawecki and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021764617&doi=10.5586%2faa.1701&partnerID=40&md5=08218efe0b5c44652666a966d3d5eeee},

doi = {10.5586/aa.1701},

issn = {00650951},

year  = {2017},

date = {2017-01-01},

journal = {Acta Agrobotanica},

volume = {70},

number = {2},

publisher = {Polish Botanical Society},

abstract = {Juglone is a substance that limits plant growth and has a toxic effect on plant development. In this study, we analyzed the influence of juglone at two different concentrations (10-3 M and 10-4 M), which were applied to different parts of Solanum lycopersicum L. plants (root system; stem after decapitation; and surface of a younger leaf or after autografting) for a short period of time (7 days), on the morphology and histology of stems. At a lower concentration, juglone had positive effects on plant growth, which resulted in an increase in interfascicular cambial cell divisions, faster development of a continuous cambium layer along the stem circumference, and development of fibers. Additionally, under the influence of juglone, the number of developing leaves increased and adventitious roots developed. The results are discussed based on the current literature concerning the reaction of plants to juglone and to stress conditions. © The Author(s) 2017.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84879842940,

title = {Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana},

author = { K. Sala and I.W. Potocka and E.U. Kurczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84879842940&doi=10.1007%2fs10535-013-0304-6&partnerID=40&md5=6d23dd96351eb5038e6f5123ed9959f0},

doi = {10.1007/s10535-013-0304-6},

issn = {00063134},

year  = {2013},

date = {2013-01-01},

journal = {Biologia Plantarum},

volume = {57},

number = {3},

pages = {410-416},

abstract = {The aim of the present study was to describe the occurrence of three pectic epitopes, recognized by JIM7, LM19, and LM5 antibodies, during somatic (SE) and zygotic (ZE) embryogenesis in Arabidopsis thaliana. The epitopes recognized by JIM7 and LM19 antibodies showed different distributions during SE stages. Moreover, in the early stages of somatic embryo development, a cytoplasmic occurrence of LM19 epitope was detected. Distribution of a pectic epitope recognized by LM5 antibody corresponded to a vascular system differentiation pattern. Occurrence of LM5 epitope was the same in both zygotic and somatic embryos and often restricted to newly synthesized walls of two adjacent cells. These data suggest that both low and high methyl-esterified pectins (recognized by LM19 and JIM7 antibodies; respectively) are developmentally regulated during SE stages and (1→4)-β-D-galactan epitope (recognized by LM5 antibody) may play a role in cell cytokinesis. © 2013 Springer Science+Business Media Dordrecht.},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}