• mgr Paulina Biniecka
Stanowisko: Biolog
Jednostka: Wydział Nauk Przyrodniczych
Adres: 40-032 Katowice, ul. Jagiellońska 28
Piętro: parter
Numer pokoju: A-31a
Telefon: (32) 2009 566
E-mail: paulina.biniecka@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 57194390781
Publikacje z bazy Scopus
2023
Fratta, S.; Biniecka, P.; Moreno-Vargas, A. J.; Carmona, A. T.; Nahimana, A.; Duchosal, M. A.; Piacente, F.; Bruzzone, S.; Caffa, I.; Nencioni, A.; Robina, I.
In: European Journal of Medicinal Chemistry, vol. 250, 2023, ISSN: 02235234.
@article{2-s2.0-85147865384,
title = {Synthesis and structure-activity relationship of new nicotinamide phosphoribosyltransferase inhibitors with antitumor activity on solid and haematological cancer},
author = { S. Fratta and P. Biniecka and A.J. Moreno-Vargas and A.T. Carmona and A. Nahimana and M.A. Duchosal and F. Piacente and S. Bruzzone and I. Caffa and A. Nencioni and I. Robina},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85147865384&doi=10.1016%2fj.ejmech.2023.115170&partnerID=40&md5=3ddaa7d9bbaa41f6720ca25ec22db8f4},
doi = {10.1016/j.ejmech.2023.115170},
issn = {02235234},
year = {2023},
date = {2023-01-01},
journal = {European Journal of Medicinal Chemistry},
volume = {250},
publisher = {Elsevier Masson s.r.l.},
abstract = {Cancer cells are highly dependent on Nicotinamide phosphoribosyltransferase (NAMPT) activity for proliferation, therefore NAMPT represents an interesting target for the development of anti-cancer drugs. Several compounds, such as FK866 and CHS828, were identified as potent NAMPT inhibitors with strong anti-cancer activity, although none of them reached the late stages of clinical trials. We present herein the preparation of three libraries of new inhibitors containing (pyridin-3-yl)triazole, (pyridin-3-yl)thiourea and (pyridin-3/4-yl)cyanoguanidine as cap/connecting unit and a furyl group at the tail position of the compound. Antiproliferative activity in vitro was evaluated on a panel of solid and haematological cancer cell lines and most of the synthesized compounds showed nanomolar or sub-nanomolar cytotoxic activity in MiaPaCa-2 (pancreatic cancer), ML2 (acute myeloid leukemia), JRKT (acute lymphobalistic leukemia), NMLW (Burkitt lymphoma), RPMI8226 (multiple myeloma) and NB4 (acute myeloid leukemia), with lower IC50 values than those reported for FK866. Notably, compounds 35a, 39a and 47 showed cytotoxic activity against ML2 with IC50 = 18, 46 and 49 pM, and IC50 towards MiaPaCa-2 of 0.005, 0.455 and 2.81 nM, respectively. Moreover, their role on the NAD+ synthetic pathway was demonstrated by the NAMPT inhibition assay. Finally, the intracellular NAD+ depletion was confirmed in vitro to induced ROS accumulation that cause a time-dependent mitochondrial membrane depolarization, leading to ATP loss and cell death. © 2023 The Authors},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Cancer cells are highly dependent on Nicotinamide phosphoribosyltransferase (NAMPT) activity for proliferation, therefore NAMPT represents an interesting target for the development of anti-cancer drugs. Several compounds, such as FK866 and CHS828, were identified as potent NAMPT inhibitors with strong anti-cancer activity, although none of them reached the late stages of clinical trials. We present herein the preparation of three libraries of new inhibitors containing (pyridin-3-yl)triazole, (pyridin-3-yl)thiourea and (pyridin-3/4-yl)cyanoguanidine as cap/connecting unit and a furyl group at the tail position of the compound. Antiproliferative activity in vitro was evaluated on a panel of solid and haematological cancer cell lines and most of the synthesized compounds showed nanomolar or sub-nanomolar cytotoxic activity in MiaPaCa-2 (pancreatic cancer), ML2 (acute myeloid leukemia), JRKT (acute lymphobalistic leukemia), NMLW (Burkitt lymphoma), RPMI8226 (multiple myeloma) and NB4 (acute myeloid leukemia), with lower IC50 values than those reported for FK866. Notably, compounds 35a, 39a and 47 showed cytotoxic activity against ML2 with IC50 = 18, 46 and 49 pM, and IC50 towards MiaPaCa-2 of 0.005, 0.455 and 2.81 nM, respectively. Moreover, their role on the NAD+ synthetic pathway was demonstrated by the NAMPT inhibition assay. Finally, the intracellular NAD+ depletion was confirmed in vitro to induced ROS accumulation that cause a time-dependent mitochondrial membrane depolarization, leading to ATP loss and cell death. © 2023 The Authors
Biniecka, P.; Matsumoto, S.; Belotti, A.; Joussot, J.; Bai, Ji.; Majjigapu, S. R.; Thoueille, P.; Spaggiari, D.; Desfontaine, V.; Piacente, F.; Bruzzone, S.; Cea, M.; Décosterd, L. A.; Vogel, P.; Nencioni, A.; Duchosal, M. A.; Nahimana, A.
Anticancer Activities of Novel Nicotinamide Phosphoribosyltransferase Inhibitors in Hematological Malignancies Journal Article
In: Molecules, vol. 28, no. 4, 2023, ISSN: 14203049.
@article{2-s2.0-85149053523,
title = {Anticancer Activities of Novel Nicotinamide Phosphoribosyltransferase Inhibitors in Hematological Malignancies},
author = { P. Biniecka and S. Matsumoto and A. Belotti and J. Joussot and Ji. Bai and S.R. Majjigapu and P. Thoueille and D. Spaggiari and V. Desfontaine and F. Piacente and S. Bruzzone and M. Cea and L.A. Décosterd and P. Vogel and A. Nencioni and M.A. Duchosal and A. Nahimana},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149053523&doi=10.3390%2fmolecules28041897&partnerID=40&md5=ee1019103a6af67443f87dbfd33f9129},
doi = {10.3390/molecules28041897},
issn = {14203049},
year = {2023},
date = {2023-01-01},
journal = {Molecules},
volume = {28},
number = {4},
publisher = {MDPI},
abstract = {Targeting cancer cells that are highly dependent on the nicotinamide adenine dinucleotide (NAD+) metabolite is a promising therapeutic strategy. Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme catalyzing NAD+ production. Despite the high efficacy of several developed NAMPT inhibitors (i.e.; FK866 (APO866)) in preclinical studies, their clinical activity was proven to be limited. Here, we report the synthesis of new NAMPT Inhibitors, JJ08, FEI191 and FEI199, which exhibit a broad anticancer activity in vitro. Results show that these compounds are potent NAMPT inhibitors that deplete NAD+ and NADP(H) after 24 h of drug treatment, followed by an increase in reactive oxygen species (ROS) accumulation. The latter event leads to ATP loss and mitochondrial depolarization with induction of apoptosis and necrosis. Supplementation with exogenous NAD+ precursors or catalase (ROS scavenger) abrogates the cell death induced by the new compounds. Finally, in vivo administration of the new NAMPT inhibitors in a mouse xenograft model of human Burkitt lymphoma delays tumor growth and significantly prolongs mouse survival. The most promising results are collected with JJ08, which completely eradicates tumor growth. Collectively, our findings demonstrate the efficient anticancer activity of the new NAMPT inhibitor JJ08 and highlight a strong interest for further evaluation of this compound in hematological malignancies. © 2023 by the authors.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Targeting cancer cells that are highly dependent on the nicotinamide adenine dinucleotide (NAD+) metabolite is a promising therapeutic strategy. Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme catalyzing NAD+ production. Despite the high efficacy of several developed NAMPT inhibitors (i.e.; FK866 (APO866)) in preclinical studies, their clinical activity was proven to be limited. Here, we report the synthesis of new NAMPT Inhibitors, JJ08, FEI191 and FEI199, which exhibit a broad anticancer activity in vitro. Results show that these compounds are potent NAMPT inhibitors that deplete NAD+ and NADP(H) after 24 h of drug treatment, followed by an increase in reactive oxygen species (ROS) accumulation. The latter event leads to ATP loss and mitochondrial depolarization with induction of apoptosis and necrosis. Supplementation with exogenous NAD+ precursors or catalase (ROS scavenger) abrogates the cell death induced by the new compounds. Finally, in vivo administration of the new NAMPT inhibitors in a mouse xenograft model of human Burkitt lymphoma delays tumor growth and significantly prolongs mouse survival. The most promising results are collected with JJ08, which completely eradicates tumor growth. Collectively, our findings demonstrate the efficient anticancer activity of the new NAMPT inhibitor JJ08 and highlight a strong interest for further evaluation of this compound in hematological malignancies. © 2023 by the authors.
Matsumoto, S.; Biniecka, P.; Bellotti, A.; Duchosal, M. A.; Nahimana, A.
Nicotinaldehyde, a Novel Precursor of NAD Biosynthesis, Abrogates the Anti-Cancer Activity of an NAD-Lowering Agent in Leukemia Journal Article
In: Cancers, vol. 15, no. 3, 2023, ISSN: 20726694.
@article{2-s2.0-85147877984,
title = {Nicotinaldehyde, a Novel Precursor of NAD Biosynthesis, Abrogates the Anti-Cancer Activity of an NAD-Lowering Agent in Leukemia},
author = { S. Matsumoto and P. Biniecka and A. Bellotti and M.A. Duchosal and A. Nahimana},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85147877984&doi=10.3390%2fcancers15030787&partnerID=40&md5=852aee0d6d585290f46f407370fb954e},
doi = {10.3390/cancers15030787},
issn = {20726694},
year = {2023},
date = {2023-01-01},
journal = {Cancers},
volume = {15},
number = {3},
publisher = {MDPI},
abstract = {Targeting NAD depletion in cancer cells has emerged as an attractive therapeutic strategy for cancer treatment, based on the higher reliance of malignant vs. healthy cells on NAD to sustain their aberrant proliferation and altered metabolism. NAD depletion is exquisitely observed when NAMPT, a key enzyme for the biosynthesis of NAD, is inhibited. Growing evidence suggests that alternative NAD sources present in a tumor environment can bypass NAMPT and render its inhibition ineffective. Here, we report the identification of nicotinaldehyde as a novel precursor that can be used for NAD biosynthesis by human leukemia cells. Nicotinaldehyde supplementation replenishes the intracellular NAD level in leukemia cells treated with NAMPT inhibitor APO866 and prevents APO866-induced oxidative stress, mitochondrial dysfunction and ATP depletion. We show here that NAD biosynthesis from nicotinaldehyde depends on NAPRT and occurs via the Preiss–Handler pathway. The availability of nicotinaldehyde in a tumor environment fully blunts the antitumor activity of APO866 in vitro and in vivo. This is the first study to report the role of nicotinaldehyde in the NAD-targeted anti-cancer treatment, highlighting the importance of the tumor metabolic environment in modulating the efficacy of NAD-lowering cancer therapy. © 2023 by the authors.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Targeting NAD depletion in cancer cells has emerged as an attractive therapeutic strategy for cancer treatment, based on the higher reliance of malignant vs. healthy cells on NAD to sustain their aberrant proliferation and altered metabolism. NAD depletion is exquisitely observed when NAMPT, a key enzyme for the biosynthesis of NAD, is inhibited. Growing evidence suggests that alternative NAD sources present in a tumor environment can bypass NAMPT and render its inhibition ineffective. Here, we report the identification of nicotinaldehyde as a novel precursor that can be used for NAD biosynthesis by human leukemia cells. Nicotinaldehyde supplementation replenishes the intracellular NAD level in leukemia cells treated with NAMPT inhibitor APO866 and prevents APO866-induced oxidative stress, mitochondrial dysfunction and ATP depletion. We show here that NAD biosynthesis from nicotinaldehyde depends on NAPRT and occurs via the Preiss–Handler pathway. The availability of nicotinaldehyde in a tumor environment fully blunts the antitumor activity of APO866 in vitro and in vivo. This is the first study to report the role of nicotinaldehyde in the NAD-targeted anti-cancer treatment, highlighting the importance of the tumor metabolic environment in modulating the efficacy of NAD-lowering cancer therapy. © 2023 by the authors.
Lewandowski, Ma.; Biniecka, P.
Polyester polyols based on aromatic alcohols [Poliole poliestrowe na bazie alkoholi aromatycznych] Journal Article
In: Przemysl Chemiczny, vol. 102, no. 2, pp. 152-155, 2023, ISSN: 00332496.
@article{2-s2.0-85169802495,
title = {Polyester polyols based on aromatic alcohols [Poliole poliestrowe na bazie alkoholi aromatycznych]},
author = { Ma. Lewandowski and P. Biniecka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85169802495&doi=10.15199%2f62.2023.2.1&partnerID=40&md5=bf54d764397e59cd0778a2dd7e968834},
doi = {10.15199/62.2023.2.1},
issn = {00332496},
year = {2023},
date = {2023-01-01},
journal = {Przemysl Chemiczny},
volume = {102},
number = {2},
pages = {152-155},
publisher = {Wydawnictwo SIGMA-NOT},
abstract = {Polyester polyols based on phthalic anhydride or a mixt. of carboxylic acids and resorcinol were obtained, and then polyurethane foams were prepared from them. To modify the flammability properties, the addn. of tetrabromophthalate diol (PTH-diol) was used at the stage of foam production or at the stage of polyester polyol synthesis, incorporating the PTH-diol molecule into the polyester polyol chain. The simultaneous use of aromatic alc. and Br flame retardant for the synthesis of polyester polyol allowed to maintain or reduce the single burning item indexes SBI of the polyurethane foam produced from it, while creating a favorable scale on the surface of the burnt sample. The obtained polyurethane foams were characterized by reduced maximum rate of heat emission, MARHE and heat release rate, HRR and increased heat of combustion. © 2023, Wydawnictwo SIGMA-NOT. All rights reserved.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Polyester polyols based on phthalic anhydride or a mixt. of carboxylic acids and resorcinol were obtained, and then polyurethane foams were prepared from them. To modify the flammability properties, the addn. of tetrabromophthalate diol (PTH-diol) was used at the stage of foam production or at the stage of polyester polyol synthesis, incorporating the PTH-diol molecule into the polyester polyol chain. The simultaneous use of aromatic alc. and Br flame retardant for the synthesis of polyester polyol allowed to maintain or reduce the single burning item indexes SBI of the polyurethane foam produced from it, while creating a favorable scale on the surface of the burnt sample. The obtained polyurethane foams were characterized by reduced maximum rate of heat emission, MARHE and heat release rate, HRR and increased heat of combustion. © 2023, Wydawnictwo SIGMA-NOT. All rights reserved.
2022
Lewandowski, Ma.; Biniecka, P.
Method of purification of post-production condensates from polyester polyol production Journal Article
In: Polish Journal of Chemical Technology, vol. 24, no. 4, pp. 78-83, 2022, ISSN: 15098117.
@article{2-s2.0-85145607300,
title = {Method of purification of post-production condensates from polyester polyol production},
author = { Ma. Lewandowski and P. Biniecka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145607300&doi=10.2478%2fpjct-2022-0032&partnerID=40&md5=2067d35812c945c8f1fe550fae629e5a},
doi = {10.2478/pjct-2022-0032},
issn = {15098117},
year = {2022},
date = {2022-01-01},
journal = {Polish Journal of Chemical Technology},
volume = {24},
number = {4},
pages = {78-83},
publisher = {Sciendo},
abstract = {Nowadays, the topics of closed-loop and eco-design are raised very often, especially in the chemical industry. To combine development with these trends, Purinova Sp. z o.o. has focused on pursuing the closed-loop use of post-production condensate from polyester polyols production. To this end, purification and distillation processes have been adapted, both at the laboratory and production scale, to receive treated condensate with decreased Chemical Oxygen Demand (COD) index. The method involves connected purification of production condensate by returning condensate to the top of the distillation column during polycondensation and two stages distillation system afterwards. The method allows for decreasing COD index and contents of diethylene glycol and 1,4-dioxane. The resulting technology has consequently allowed the use of tailored distillation in the purification of post-production condensates in the production of polyester polyols. Furthermore, the quality of the condensate obtained allowed it to be used in the closed loop of the production plant. © 2022 Marek Lewandowski et al., published by Sciendo.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Nowadays, the topics of closed-loop and eco-design are raised very often, especially in the chemical industry. To combine development with these trends, Purinova Sp. z o.o. has focused on pursuing the closed-loop use of post-production condensate from polyester polyols production. To this end, purification and distillation processes have been adapted, both at the laboratory and production scale, to receive treated condensate with decreased Chemical Oxygen Demand (COD) index. The method involves connected purification of production condensate by returning condensate to the top of the distillation column during polycondensation and two stages distillation system afterwards. The method allows for decreasing COD index and contents of diethylene glycol and 1,4-dioxane. The resulting technology has consequently allowed the use of tailored distillation in the purification of post-production condensates in the production of polyester polyols. Furthermore, the quality of the condensate obtained allowed it to be used in the closed loop of the production plant. © 2022 Marek Lewandowski et al., published by Sciendo.
Dąbrowska, D.; Nowak, A.; Sołtysiak, M.; Biniecka, P.; Nourani, V.; Wasilkowski, D.
In situ lysimeter experiment of leaching pollutants from municipal waste with physicochemical status and microbiome condition Journal Article
In: Journal of Hydrology, vol. 613, 2022, ISSN: 00221694.
@article{2-s2.0-85136709506,
title = {In situ lysimeter experiment of leaching pollutants from municipal waste with physicochemical status and microbiome condition},
author = { D. Dąbrowska and A. Nowak and M. Sołtysiak and P. Biniecka and V. Nourani and D. Wasilkowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136709506&doi=10.1016%2fj.jhydrol.2022.128309&partnerID=40&md5=86b0fd0a3fdccec0f1355a3f60e2397a},
doi = {10.1016/j.jhydrol.2022.128309},
issn = {00221694},
year = {2022},
date = {2022-01-01},
journal = {Journal of Hydrology},
volume = {613},
publisher = {Elsevier B.V.},
abstract = {Lysimeter tests are an ideal supplement to monitoring tests performed in the area of landfills. This article presents an interdisciplinary lysimeter experiment that used hydrogeological, biochemical, and microbiological studies to evaluate the process of leaching pollutants from waste. The obtained results of leachate tests indicate that the EC value was as high as 31 mS/cm which corresponds to poor water quality. Additionally, high concentrations of chlorides (up to 5095 mg/L) and sulphates (up to 10107 mg/L) were observed. The results of microbiological tests confirm the seasonality. The statistically significant (p < 0.05) difference in the number of heterotrophic bacteria was denoted between autumn (1.6·107 CFU cm-3) and winter (1.5·105 CFU cm-3.The analysis of the ability to utilize nitrogen and phosphorus sources showed seasonal differences in the use of substrates containing these biogenic elements. It was observed that nitrogen-containing compounds were most intensively used in winter and the least in spring while phosphorus compounds were the most intensively oxidized in summer. Presented results confirm that lysimeter studies can play a valuable role in the construction of landfills for the best method of waste isolation and limiting the growth of microorganisms. © 2022 The Author(s)},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Lysimeter tests are an ideal supplement to monitoring tests performed in the area of landfills. This article presents an interdisciplinary lysimeter experiment that used hydrogeological, biochemical, and microbiological studies to evaluate the process of leaching pollutants from waste. The obtained results of leachate tests indicate that the EC value was as high as 31 mS/cm which corresponds to poor water quality. Additionally, high concentrations of chlorides (up to 5095 mg/L) and sulphates (up to 10107 mg/L) were observed. The results of microbiological tests confirm the seasonality. The statistically significant (p < 0.05) difference in the number of heterotrophic bacteria was denoted between autumn (1.6·107 CFU cm-3) and winter (1.5·105 CFU cm-3.The analysis of the ability to utilize nitrogen and phosphorus sources showed seasonal differences in the use of substrates containing these biogenic elements. It was observed that nitrogen-containing compounds were most intensively used in winter and the least in spring while phosphorus compounds were the most intensively oxidized in summer. Presented results confirm that lysimeter studies can play a valuable role in the construction of landfills for the best method of waste isolation and limiting the growth of microorganisms. © 2022 The Author(s)
ElMokh, O.; Matsumoto, S.; Biniecka, P.; Bellotti, A.; Schaeuble, K.; Piacente, F.; Gallart-Ayala, H.; Ivanisevic, J.; Stamenkovic, I.; Nencioni, A.; Nahimana, A.; Duchosal, M. A.
Gut microbiota severely hampers the efficacy of NAD-lowering therapy in leukemia Journal Article
In: Cell Death and Disease, vol. 13, no. 4, 2022, ISSN: 20414889, (3).
@article{2-s2.0-85127927168,
title = {Gut microbiota severely hampers the efficacy of NAD-lowering therapy in leukemia},
author = { O. ElMokh and S. Matsumoto and P. Biniecka and A. Bellotti and K. Schaeuble and F. Piacente and H. Gallart-Ayala and J. Ivanisevic and I. Stamenkovic and A. Nencioni and A. Nahimana and M.A. Duchosal},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127927168&doi=10.1038%2fs41419-022-04763-3&partnerID=40&md5=ada058e1b3147adade66aa7b08372f2c},
doi = {10.1038/s41419-022-04763-3},
issn = {20414889},
year = {2022},
date = {2022-01-01},
journal = {Cell Death and Disease},
volume = {13},
number = {4},
publisher = {Springer Nature},
abstract = {Most cancer cells have high need for nicotinamide adenine dinucleotide (NAD+) to sustain their survival. This led to the development of inhibitors of nicotinamide (NAM) phosphoribosyltransferase (NAMPT), the rate-limiting NAD+ biosynthesis enzyme from NAM. Such inhibitors kill cancer cells in preclinical studies but failed in clinical ones. To identify parameters that could negatively affect the therapeutic efficacy of NAMPT inhibitors and propose therapeutic strategies to circumvent such failure, we performed metabolomics analyses in tumor environment and explored the effect of the interaction between microbiota and cancer cells. Here we show that tumor environment enriched in vitamin B3 (NAM) or nicotinic acid (NA) significantly lowers the anti-tumor efficacy of APO866, a prototypic NAMPT inhibitor. Additionally, bacteria (from the gut; or in the medium) can convert NAM into NA and thus fuel an alternative NAD synthesis pathway through NA. This leads to the rescue from NAD depletion, prevents reactive oxygen species production, preserves mitochondrial integrity, blunts ATP depletion, and protects cancer cells from death. Our data in an in vivo preclinical model reveal that antibiotic therapy down-modulating gut microbiota can restore the anti-cancer efficacy of APO866. Alternatively, NAphosphoribosyltransferase inhibition may restore anti-cancer activity of NAMPT inhibitors in the presence of gut microbiota and of NAM in the diet. © 2022, The Author(s).},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Most cancer cells have high need for nicotinamide adenine dinucleotide (NAD+) to sustain their survival. This led to the development of inhibitors of nicotinamide (NAM) phosphoribosyltransferase (NAMPT), the rate-limiting NAD+ biosynthesis enzyme from NAM. Such inhibitors kill cancer cells in preclinical studies but failed in clinical ones. To identify parameters that could negatively affect the therapeutic efficacy of NAMPT inhibitors and propose therapeutic strategies to circumvent such failure, we performed metabolomics analyses in tumor environment and explored the effect of the interaction between microbiota and cancer cells. Here we show that tumor environment enriched in vitamin B3 (NAM) or nicotinic acid (NA) significantly lowers the anti-tumor efficacy of APO866, a prototypic NAMPT inhibitor. Additionally, bacteria (from the gut; or in the medium) can convert NAM into NA and thus fuel an alternative NAD synthesis pathway through NA. This leads to the rescue from NAD depletion, prevents reactive oxygen species production, preserves mitochondrial integrity, blunts ATP depletion, and protects cancer cells from death. Our data in an in vivo preclinical model reveal that antibiotic therapy down-modulating gut microbiota can restore the anti-cancer efficacy of APO866. Alternatively, NAphosphoribosyltransferase inhibition may restore anti-cancer activity of NAMPT inhibitors in the presence of gut microbiota and of NAM in the diet. © 2022, The Author(s).
2019
Biniecka, P.; Pieńkowska, M.; Opalska, A.
In: Polimery/Polymers, vol. 64, no. 3, pp. 199-207, 2019, ISSN: 00322725.
@article{2-s2.0-85063299868,
title = {Innovative method to prepare polyester polyols intended for the production of footwear soles [Innowacyjna metoda otrzymywania polioli poliestrowych przeznaczonych do produkcji spodów obuwniczych]},
author = { P. Biniecka and M. Pieńkowska and A. Opalska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063299868&doi=10.14314%2fpolimery.2019.3.5&partnerID=40&md5=8ecf4a8cdc2b495a0ff73a3f3e6f057a},
doi = {10.14314/polimery.2019.3.5},
issn = {00322725},
year = {2019},
date = {2019-01-01},
journal = {Polimery/Polymers},
volume = {64},
number = {3},
pages = {199-207},
publisher = {Industrial Chemistry Research Institute},
abstract = {A new technology for the production of polyester polyols using a newly separated side stream from the cyclohexane oxidation process as a raw material was developed. The innovation consisted in the introduction of the stage of separation of the waste product stream, which allowed to obtain a fraction with composition suitable to ensure optimal properties of the synthesized polyol intended for polyurethane systems used in the production of footwear soles. The obtained prepolyester fraction was characterized by a high content of bifunctional aliphatic compounds, with a minor share of low molecular weight monoacids, contributing to the deterioration of final product quality. The fraction resulting from the separation is an alternative raw material to the commonly used adipic acid. The parameters of footwear soles manufactured from microporous polyurethanes based on the obtained polyesters were evaluated. It was found that polyesters produced from new raw material are characterized by similar or higher physical and mechanical properties in comparison to the existing products of Purinova Company. 2019 © Industrial Chemistry Research Institute. All Rights Reserved.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A new technology for the production of polyester polyols using a newly separated side stream from the cyclohexane oxidation process as a raw material was developed. The innovation consisted in the introduction of the stage of separation of the waste product stream, which allowed to obtain a fraction with composition suitable to ensure optimal properties of the synthesized polyol intended for polyurethane systems used in the production of footwear soles. The obtained prepolyester fraction was characterized by a high content of bifunctional aliphatic compounds, with a minor share of low molecular weight monoacids, contributing to the deterioration of final product quality. The fraction resulting from the separation is an alternative raw material to the commonly used adipic acid. The parameters of footwear soles manufactured from microporous polyurethanes based on the obtained polyesters were evaluated. It was found that polyesters produced from new raw material are characterized by similar or higher physical and mechanical properties in comparison to the existing products of Purinova Company. 2019 © Industrial Chemistry Research Institute. All Rights Reserved.
2018
Biniecka, P.; Pieńkowska, M.
Innovative method for obtaining synthetic esters [Innowacyjna metoda otrzymywania estrów syntetycznych] Journal Article
In: Przemysl Chemiczny, vol. 97, no. 10, pp. 1645-1648, 2018, ISSN: 00332496.
@article{2-s2.0-85063331407,
title = {Innovative method for obtaining synthetic esters [Innowacyjna metoda otrzymywania estrów syntetycznych]},
author = { P. Biniecka and M. Pieńkowska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063331407&doi=10.15199%2f62.2018.10.3&partnerID=40&md5=cd5032bbe7df892617920bd475685f69},
doi = {10.15199/62.2018.10.3},
issn = {00332496},
year = {2018},
date = {2018-01-01},
journal = {Przemysl Chemiczny},
volume = {97},
number = {10},
pages = {1645-1648},
publisher = {Wydawnictwo SIGMA-NOT},
abstract = {Carboxylic acids-contg. by-product from com. oxidn. of cyclohexane was enriched in the acids by phase sepn. and simple distn., analyzed for chem. compn. and used for esterification with pentaeritritol in presence of SnCl 2 . The esters were studied for viscosity, flow flash point, temp., hydroxyl and acid nos. and biodegradability. The esters met the quality requirements and were recommended as bases for prodn. of synthetic lubricating oils. © 2018, Wydawnictwo SIGMA-NOT. All rights reserved.},
keywords = {},
pubstate = {published},
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Carboxylic acids-contg. by-product from com. oxidn. of cyclohexane was enriched in the acids by phase sepn. and simple distn., analyzed for chem. compn. and used for esterification with pentaeritritol in presence of SnCl 2 . The esters were studied for viscosity, flow flash point, temp., hydroxyl and acid nos. and biodegradability. The esters met the quality requirements and were recommended as bases for prodn. of synthetic lubricating oils. © 2018, Wydawnictwo SIGMA-NOT. All rights reserved.
2017
Jaworski, S.; Biniecka, P.; Bugajska, Ż.; Daniluk, K.; Dyjak, S.; Strojny, B.; Kutwin, M.; Wierzbicki, M.; Grodzik, M.; Chwalibog, A.
Analysis of the cytotoxicity of hierarchical nanoporous graphenic carbon against human glioblastoma grade IV cells Journal Article
In: International Journal of Nanomedicine, vol. 12, pp. 3839-3849, 2017, ISSN: 11769114, (3).
@article{2-s2.0-85019859715,
title = {Analysis of the cytotoxicity of hierarchical nanoporous graphenic carbon against human glioblastoma grade IV cells},
author = { S. Jaworski and P. Biniecka and Ż. Bugajska and K. Daniluk and S. Dyjak and B. Strojny and M. Kutwin and M. Wierzbicki and M. Grodzik and A. Chwalibog},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019859715&doi=10.2147%2fIJN.S135932&partnerID=40&md5=02f157e3fbfabaef431809f34d38026c},
doi = {10.2147/IJN.S135932},
issn = {11769114},
year = {2017},
date = {2017-01-01},
journal = {International Journal of Nanomedicine},
volume = {12},
pages = {3839-3849},
publisher = {Dove Medical Press Ltd.},
abstract = {A newly produced hierarchical, nanoporous carbon (HNC) material is studied for the first time in a biological model. The material consists of uniform particles and is characterized by a mean diameter <150 nm, a high specific surface area of 1,000 m2/g, well-developed porosity, and high electrical conductivity. These unique properties and ability to transfer charge create a possibility of employing HNC as a moderator of tumor cell growth. As the charge of HNC may interfere with cell membranes by adhesion and by bonding with cell receptors, it may block the supply of nutrients. The interactions of HNC with the U87 cells can also lead to the excessive generation of reactive oxygen species (ROS) and activate apoptotic mechanisms in cancer cells. The investigation was performed using U87 human glioblastoma and PCS-201–010 normal fibroblast cell lines, where cell morphology and ultrastructure, viability, ROS production, type of cell death, mitochondrial transmembrane potential, and the expression of genes engaged in apoptosis pathways are studied. The results demonstrate that cytotoxicity of HNC particles increases with concentration from 5 to 100 μg/mL by activation of apoptosis through the mitochondrial pathway, without inducing necrosis. Our research indicates the potential applicability of HNC in cancer therapy. © 2017 Jaworski et al.},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A newly produced hierarchical, nanoporous carbon (HNC) material is studied for the first time in a biological model. The material consists of uniform particles and is characterized by a mean diameter <150 nm, a high specific surface area of 1,000 m2/g, well-developed porosity, and high electrical conductivity. These unique properties and ability to transfer charge create a possibility of employing HNC as a moderator of tumor cell growth. As the charge of HNC may interfere with cell membranes by adhesion and by bonding with cell receptors, it may block the supply of nutrients. The interactions of HNC with the U87 cells can also lead to the excessive generation of reactive oxygen species (ROS) and activate apoptotic mechanisms in cancer cells. The investigation was performed using U87 human glioblastoma and PCS-201–010 normal fibroblast cell lines, where cell morphology and ultrastructure, viability, ROS production, type of cell death, mitochondrial transmembrane potential, and the expression of genes engaged in apoptosis pathways are studied. The results demonstrate that cytotoxicity of HNC particles increases with concentration from 5 to 100 μg/mL by activation of apoptosis through the mitochondrial pathway, without inducing necrosis. Our research indicates the potential applicability of HNC in cancer therapy. © 2017 Jaworski et al.