@article{2-s2.0-85211618567,

title = {The paradoxical effects of beneficial bacteria on Solanum lycopersicum under Cd stress},

author = { M. Noszczyńska and M. Pawlik and M. Rudnicka and D. Wójcik and M. Gajecka and K. Kukucz and M. Skowronek and I.W. Potocka and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85211618567&doi=10.1016%2fj.envpol.2024.125430&partnerID=40&md5=4ce49372be49e0ed95cdb86bd4376fe1},

doi = {10.1016/j.envpol.2024.125430},

year  = {2025},

date = {2025-01-01},

journal = {Environmental Pollution},

volume = {366},

publisher = {Elsevier Ltd},

abstract = {This study investigated the complex interactions between a novel consortium and tomato seedlings under cadmium (Cd) stress. The consortium consists of two bacteria, Pseudomonas sp. HS4 and Paenarthrobacter sp. AS8, both with proven plant growth-promoting (PGP) properties, isolated from Cd hyperaccumulators. Our research highlights the paradoxical effects of these bacteria, revealing their dual role in reducing Cd uptake while simultaneously inducing oxidative stress in plants. Hydroponic experiments showed that the consortium reduced Cd accumulation in tomato shoots by 52% compared to uninoculated controls. However, this reduction was accompanied by decreased plant biomass and increased oxidative stress, with malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) levels up to 80% and 160% higher, respectively, in inoculated plants. Root H₂O₂ production increased by 38% under 50 μM Cd without a corresponding rise in catalase (CAT) activity. Despite Cd exposure, the consortium promoted chlorophyll and carotenoid synthesis, restoring pigment levels to those of unstressed controls. Gene expression analysis revealed a complex impact on stress responses, with inoculation suppressing Sl1 gene expression in roots and upregulating the oxidative stress-related GR-1 gene in shoots. These findings highlight the complex and multifaceted relationship between beneficial bacteria and plant fitness under heavy metal stress, with significant implications for sustainable agriculture. The study raises new questions regarding the broader physiological and ecological impacts of applying hyperaccumulator-associated bacteria in crop management, emphasizing the necessity for deeper mechanistic insights into these interactions to fully harness their potential in improving crop resilience and productivity. © 2024 Elsevier Ltd},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85203410085,

title = {Structure of the O-specific polysaccharide of Asaia bogorensis ATCC BAA-21 lipopolysaccharide},

author = { A. Kaczmarek and A. Maciejewska and K. Kasperkiewicz and M. Noszczyńska and J. Łukasiewicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203410085&doi=10.1016%2fj.carres.2024.109266&partnerID=40&md5=58676ae5256daabb01ebdd0c3e0f35fa},

doi = {10.1016/j.carres.2024.109266},

year  = {2024},

date = {2024-01-01},

journal = {Carbohydrate Research},

volume = {545},

publisher = {Elsevier Ltd},

abstract = {Asaia bogorensis is a Gram-negative bacterium isolated from flowers and fruits growing in tropical climate, reproductive system of mosquitoes, and rarely from immunocompromised patients. In Europe, A. bogorensis is responsible for the contamination of flavoured mineral waters. One of the important surface antigen and an element of the bacterial biofilm is lipopolysaccharide (LPS; endotoxin). To date, no data on A. bogorensis LPS structure has been reported. Chemical analysis and 1H,13C nuclear magnetic resonance spectroscopy revealed the novel structure of the O-specific polysaccharide of A. bogorensis ATCC BAA-21 LPS. It was concluded that the repeating unit of the O-antigen is a branched trisaccharide with the following structure: →6)-α-D-Glcp-(1→2)-[β-D-Glcp-(1→3)]-α-L-Rhap-(1→. © 2024 The Author(s)},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85163682040,

title = {The microbial removal of bisphenols in aquatic microcosms and associated alteration in bacterial community},

author = { M. Noszczyńska and M. Pacwa-Płociniczak and K. Bondarczuk and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163682040&doi=10.1007%2fs11356-023-28305-2&partnerID=40&md5=db05224a4ae882bbfa3bf5f18d5f2125},

doi = {10.1007/s11356-023-28305-2},

issn = {09441344},

year  = {2023},

date = {2023-01-01},

journal = {Environmental Science and Pollution Research},

volume = {30},

number = {36},

pages = {85292-85304},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {The concept of the study resulted from numerous concerns around bisphenol A (BPA) and bisphenol S (BPS) in aquatic environments. In this study, river water and sediment microcosms highly polluted with bisphenols and bioaugmented with two BPs-removing bacterial strains were constructed. The study aimed to determine the rate of high-concentrated BPA and BPS (BPs) removal from river water and sediment microniches, and the effect of water bioaugmentation with bacterial consortium on the removal rates of these pollutants. Moreover, the impact of introduced strains and exposure to BPs on the structural and functional composition of the autochthonous bacterial communities was elucidated. Our findings indicate that the removal activity of autochthonous bacteria was sufficient for effectively BPA elimination and reducing BPS content in the microcosms. The number of introduced bacterial cells decreased continuously until day 40, and on consecutive sampling days, no bioaugmented cells were detected. Sequencing analysis of the total 16S rRNA genes revealed that the community composition in bioaugmented microcosms amended with BPs differed significantly from those treated either with bacteria or BPs. A metagenomic analysis found an increase in the abundance of proteins responsible for xenobiotics removal in BPs-amended microcosms. This study provides new insights into the effects of bioaugmentation with a bacterial consortium on bacterial diversity and BPs removal in aquatic environments. © 2023, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85132818018,

title = {Outer Membrane Vesicles as Mediators of Plant–Bacterial Interactions},

author = { M. Rudnicka and M. Noszczyńska and M. Malicka and K. Kasperkiewicz and M. Pawlik and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85132818018&doi=10.3389%2ffmicb.2022.902181&partnerID=40&md5=46f103a6c4d53db69a0da7d9312fbd41},

doi = {10.3389/fmicb.2022.902181},

issn = {1664302X},

year  = {2022},

date = {2022-01-01},

journal = {Frontiers in Microbiology},

volume = {13},

publisher = {Frontiers Media S.A.},

abstract = {Plants have co-evolved with diverse microorganisms that have developed different mechanisms of direct and indirect interactions with their host. Recently, greater attention has been paid to a direct “message” delivery pathway from bacteria to plants, mediated by the outer membrane vesicles (OMVs). OMVs produced by Gram-negative bacteria play significant roles in multiple interactions with other bacteria within the same community, the environment, and colonized hosts. The combined forces of innovative technologies and experience in the area of plant–bacterial interactions have put pressure on a detailed examination of the OMVs composition, the routes of their delivery to plant cells, and their significance in pathogenesis, protection, and plant growth promotion. This review synthesizes the available knowledge on OMVs in the context of possible mechanisms of interactions between OMVs, bacteria, and plant cells. OMVs are considered to be potential stimulators of the plant immune system, holding potential for application in plant bioprotection. Copyright © 2022 Rudnicka, Noszczyńska, Malicka, Kasperkiewicz, Pawlik and Piotrowska-Seget.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85096144505,

title = {A comprehensive study on bisphenol A degradation by newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12},

author = { M. Noszczyńska and M. Chodór and Ł. Jałowiecki and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096144505&doi=10.1007%2fs10532-020-09919-6&partnerID=40&md5=1dd7c00524c59806843995f2ec3955fc},

doi = {10.1007/s10532-020-09919-6},

issn = {09239820},

year  = {2021},

date = {2021-01-01},

journal = {Biodegradation},

volume = {32},

number = {1},

publisher = {Springer Science and Business Media B.V.},

abstract = {Bisphenol A (BPA) is an endocrine disrupting chemical. Its extensive use has led to the wide occurrence of BPA in various environmental ecosystems, at levels that may cause negative effects to the ecosystem and public health. Although there are many bacteria able to BPA utilization, only a few of them have a strong capacity for its biodegradation. Therefore, it is important to search for new bacteria strains, investigate their BPA biodegradation ability and potential effect of pH and other organic compounds on the process. These tasks have become the object of the present study. The results of our research show that for the newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12 after 15 days, with an initial BPA concentration of 100 mg L− 1, the highest BPA removal was achieved at pH 8, while sodium glutamate as a biostimulant best accelerated BPA degradation. Kinetic data for BPA biodegradation by both strains best fitted the Monod model. The specific degradation rate and the half saturation constant were estimated respectively as 8.75 mg L− 1 day− 1 and 111.27 mg L− 1 for Acinetobacter sp. K1MN, and 8.6 mg L− 1 day− 1 and 135.79 mg L− 1 for Pseudomonas sp. BG12. The half-maximal effective concentration (EC50) of BPA for Acinetobacter sp. K1MN was 120 mg L− 1 and for Pseudomonas sp. BG12 it was 123 mg L− 1. The toxicity bioassay (Microtox test) showed that elimination of BPA by both strains is accompanied by reduction of its toxic effect. The ability of tested strains to degrade BPA combined with their high resistance to this xenobiotic indicates that Acinetobacter sp. K1MN and Pseudomonas sp. BG12 are potential tools for BPA removal during wastewater treatment plant. © 2020, The Author(s).},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85089454394,

title = {White rot fungi can be a promising tool for removal of bisphenol A, bisphenol S, and nonylphenol from wastewater},

author = { A. Grelska and M. Noszczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089454394&doi=10.1007%2fs11356-020-10382-2&partnerID=40&md5=daaee536e836fe193262fede28b6c2eb},

doi = {10.1007/s11356-020-10382-2},

issn = {09441344},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Science and Pollution Research},

volume = {27},

number = {32},

pages = {39958-39976},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Endocrine-disrupting chemicals (EDC) are a wide group of chemicals that interfere with the endocrine system. Their similarity to natural steroid hormones makes them able to attach to hormone receptors, thereby causing unfavorable health effects. Among EDC, bisphenol A (BPA), bisphenol S (BPS), and nonylphenol (NP) seem to be particularly harmful. As the industry is experiencing rapid expansion, BPA, BPS, and NP are being produced in growing amounts, generating considerable environmental pollution. White rot fungi (WRF) are an economical, ecologically friendly, and socially acceptable way to remove EDC contamination from ecosystems. WRF secrete extracellular ligninolytic enzymes such as laccase, manganese peroxidase, lignin peroxidase, and versatile peroxidase, involved in lignin deterioration. Owing to the broad substrate specificity of these enzymes, they are able to remove numerous xenobiotics, including EDC. Therefore, WRF seem to be a promising tool in the abovementioned EDC elimination during wastewater treatment processes. Here, we review WRF application for this EDC removal from wastewater and indicate several strengths and limitations of such methods. © 2020, The Author(s).},

note = {29},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85089798964,

title = {A High manganese-tolerant pseudomonas sp. strain isolated from metallurgicalwaste heap can be a tool for enhancing manganese removal from contaminated soil},

author = { M. Noszczyńska and K. Łakomy and K. Nowacki and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089798964&doi=10.3390%2fapp10165717&partnerID=40&md5=fcced5b5bec5c0cdf10d6a679b4efe5c},

doi = {10.3390/app10165717},

issn = {20763417},

year  = {2020},

date = {2020-01-01},

journal = {Applied Sciences (Switzerland)},

volume = {10},

number = {16},

publisher = {MDPI AG},

abstract = {Manganese (Mn) is widely used in industry. However, its extensive applications have generated a great amount of manganese waste, which has become an ecological problem and has led to a decrease in natural resources. The use of microorganisms capable of accumulating Mn ions from contaminated ecosystems offers a potential alternative for the removal and recovery of this metal. The main aim of this work was an investigation of removal potential of Mn from soil by isolated bacterial. For this purpose, eleven bacterial strains were isolated from the soil from metallurgical waste heap in Upper Silesia, Poland. Strain named 2De with the highest Mn removal potential was selected and characterized taking into account its ability for Mn sorption and bioaccumulation from soil and medium containing manganese dioxide. Moreover, the protein profile of 2De strain before and after exposition to Mn was analyzed using SDS/PAGE technique. The 2De strain was identified as a Pseudomonas sp. The results revealed that this strain has an ability to grow at high Mn concentration and possesses an enhanced ability to remove it from the solution enriched with the soil or manganese dioxide via a biosorption mechanism. Moreover, changes in cellular protein expression of the isolated strain were observed. This study demonstrated that autochthonous 2De strain can be an effective tool to remove and recover Mn from contaminated soil. © 2020 by the authors.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85088455072,

title = {Comparative study on multiway enhanced bio- And phytoremediation of aged petroleum-contaminated soil},

author = { N. Ptaszek and M. Pacwa-Płociniczak and M. Noszczyńska and T. Płociniczak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088455072&doi=10.3390%2fagronomy10070947&partnerID=40&md5=3233ddf61e84a0d289d4a54298bf1603},

doi = {10.3390/agronomy10070947},

issn = {20734395},

year  = {2020},

date = {2020-01-01},

journal = {Agronomy},

volume = {10},

number = {7},

publisher = {MDPI AG},

abstract = {Bioremediation and phytoremediation of soil polluted with petroleum hydrocarbons (PHs) are an effective and eco-friendly alternative to physicochemical methods of soil decontamination. These techniques can be supported by the addition of effective strains and/or surface-active compounds. However, to obtain maximum efficacy of bioremediation, the interactions that occur between the microorganisms, enhancement factors and plants need to be studied. Our study aimed to investigate the removal of petroleum hydrocarbons from an aged and highly polluted soil (hydrocarbon content about 2.5%) using multiway enhanced bio- and phytoremediation. For this purpose, 10 enhanced experimental groups were compared to two untreated controls. Among the enhanced experimental groups, the bio- and phytoremediation processes were supported by the endophytic strain Rhodococcus erythropolis CDEL254. This bacterial strain has several plant growth-promoting traits and can degrade petroleum hydrocarbons and produce biosurfactants. Additionally, a rhamnolipid solution produced by Pseudomonas aeruginosa was used to support the total petroleum hydrocarbon loss from soil. After 112 days of incubation, the highest PH removal (31.1%) was observed in soil planted with ryegrass (Lolium perenne L. cv. Pearlgreen) treated with living cells of the CDEL254 strain and rhamnolipid solution. For non-planted experimental groups, the highest PH loss (26.1%) was detected for soil treated with heat-inactivated CDEL254 cells and a rhamnolipid solution. In general, the differences in the efficacy of the 10 experimental groups supported by plants, live/dead cells of the strain tested and rhamnolipid were not statistically significant. However, each of these groups was significantly more effective than the appropriate control groups. The PH loss in untreated (natural attenuation) and soils that underwent phytoremediation reached a value of 14.2% and 17.4%, respectively. Even though the CDEL254 strain colonized plant tissues and showed high survival in soil, its introduction did not significantly increase PH loss compared to systems treated with dead biomass. These results indicate that the development of effective biological techniques requires a customized approach to the polluted site and effective optimization of the methods used. © 2020 by the authors.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85042908378,

title = {Bisphenols: Application, occurrence, safety, and biodegradation mediated by bacterial communities in wastewater treatment plants and rivers},

author = { M. Noszczyńska and Z. Piotrowska-Seget},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042908378&doi=10.1016%2fj.chemosphere.2018.02.179&partnerID=40&md5=513698b7a546da494f121d268e6cdd83},

doi = {10.1016/j.chemosphere.2018.02.179},

issn = {00456535},

year  = {2018},

date = {2018-01-01},

journal = {Chemosphere},

volume = {201},

pages = {214-223},

publisher = {Elsevier Ltd},

abstract = {Numerous data indicate that most of bisphenols (BPs) are endocrine disrupters and exhibit cytotoxicity, neurotoxicity, genotoxicity and reproductive toxicity against vertebrates. Nevertheless, they are widely applied in material production what result in their ubiquitous occurrence in ecosystems. While BPA is the most frequently detected in environment, BPAF, BPF and BPS are also often found. Ecosystem particularly exposed to BPs pollution is industrial and municipal wastewater being a common source of BPA in river waters. Different techniques to remove BPs from these ecosystems have been applied, among which biodegradation seems to be the most effective. In this review the current state of knowledge in the field of BPs application, distribution in the environment, effects on animal and human health, and biodegradation mediated by bacterial populations in wastewater treatment plants and rivers is presented. © 2018 Elsevier Ltd},

note = {93},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84940451045,

title = {Interaction of human mannose-binding lectin (MBL) with Yersinia enterocolitica lipopolysaccharide},

author = { K. Kasperkiewicz and A.S. Świerzko and M.A. Bartlomiejczyk and M. Cedzyński and M. Noszczyńska and K.A. Duda and M. Michalski and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940451045&doi=10.1016%2fj.ijmm.2015.07.001&partnerID=40&md5=a13275e79aea7f4c6c6f8c15b72162af},

doi = {10.1016/j.ijmm.2015.07.001},

issn = {14384221},

year  = {2015},

date = {2015-01-01},

journal = {International Journal of Medical Microbiology},

volume = {305},

number = {6},

pages = {544-552},

publisher = {Elsevier GmbH},

abstract = {The lipopolysaccharide (LPS) is involved in the interaction between Gram-negative pathogenic bacteria and host. Mannose-binding lectin (MBL), complement-activating soluble pattern-recognition receptor targets microbial glycoconjugates, including LPS. We studied its interactions with a set of Yersinia enterocolitica O:3 LPS mutants. The wild-type strain LPS consists of lipid A (LA) substituted with an inner core oligosaccharide (IC) which in turn is substituted either with the O-specific polysaccharide (OPS) or the outer core hexasaccharide (OC), and sometimes also with the enterobacterial common antigen (ECA). The LPS mutants produced truncated LPS, missing OPS, OC or both, or, in addition, different IC constituents or ECA. MBL bound to LA-IC, LA-IC-OPS and LA-IC-ECA but not LA-IC-OC structures. Moreover, LA-IC substitution with both OPS and ECA prevented the lectin binding. Sequential truncation of the IC heptoses demonstrated that the MBL targets the IC heptose region. Furthermore, microbial growth temperature influenced MBL binding; binding was stronger to bacteria grown at room temperature (22. °C) than to bacteria grown at 37 °C. In conclusion, our results demonstrate that MBL can interact with Y. enterocolitica LPS, however, the in vivo significance of that interaction remains to be elucidated. © 2015 Elsevier GmbH.},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84938289994,

title = {Human Microbiome: When a Friend Becomes an Enemy},

author = { M. Muszer and M. Noszczyńska and K. Kasperkiewicz and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938289994&doi=10.1007%2fs00005-015-0332-3&partnerID=40&md5=db02c868f7d0ad90693c5d80b029750f},

doi = {10.1007/s00005-015-0332-3},

issn = {0004069X},

year  = {2015},

date = {2015-01-01},

journal = {Archivum Immunologiae et Therapiae Experimentalis},

volume = {63},

number = {4},

pages = {287-298},

publisher = {Birkhauser Verlag AG},

abstract = {The microorganisms that inhabit humans are very diverse on different body sites and tracts. Each specific niche contains a unique composition of the microorganisms that are important for a balanced human physiology. Microbial cells outnumber human cells by tenfold and they function as an invisible organ that is called the microbiome. Excessive use of antibiotics and unhealthy diets pose a serious danger to the composition of the microbiome. An imbalance in the microbial community may cause pathological conditions of the digestive system such as obesity, cancer and inflammatory bowel disease; of the skin such as atopic dermatitis, psoriasis and acne and of the cardiovascular system such as atherosclerosis. An unbalanced microbiome has also been associated with neurodevelopmental disorders such as autism and multiple sclerosis. While the microbiome has a strong impact on the development of the host immune system, it is suspected that it can also be the cause of certain autoimmune diseases, including diabetes or rheumatoid arthritis. Despite the enormous progress in the field, the interactions between the human body and its microbiome still remain largely unknown. A better characterization of the interactions may allow for a deeper understanding of human disease states and help to elucidate a possible association between the composition of the microbiome and certain pathologies. This review focuses on general findings that are related to the area and provides no detailed information about the case of study. The aim is to give some initial insight on the studies of the microbiome and its connection with human health. © 2015, The Author(s).},

note = {36},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84942433786,

title = {ECA - Common surface antigen of the bacilli of the Enterobacteriaceae family [ECA - Wspólny antygen powierzchniowy pałeczek rodziny Enterobacteriaceae]},

author = { K. Kasperkiewicz and M. Noszczyńska and A. Piszczek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942433786&partnerID=40&md5=1da607bf0c309be7cb88d62ca64f7da4},

issn = {00794252},

year  = {2015},

date = {2015-01-01},

journal = {Postepy Mikrobiologii},

volume = {54},

number = {2},

pages = {165-174},

publisher = {Polish Society of Microbiologists},

abstract = {Almost all the strains of bacteria belonging to the Enterobacteriaceae family share at least one common antigenic component, ECA, which is not present in other Gram-negative and Gram-positive bacteria. From the observations made with immunofluorescence and immunoferritin techniques, it has been concluded that ECA is localized in the outer leaflet of the outer membrane of Gram-negative enteric bacteria. ECA is a glycolipid consisting of linear trisaccharide repeating units composed of [→3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→]. It occurs in three structural forms: ECAPG linked to phosphatidylglycerol, ECALPS anchored to LPS core region and ECACYC not expressed on the surface. ECA is believed to be connected to the LPS outer core. However, it should be emphasized that Yersinia enterocolitica serotype O:3 mutants defective in outer core synthesis were also ECA-immunogenic. The genes involved in ECA biosynthesis are located in the chromosomal wec gene cluster, from wecA to wecG and the ECA expressions is downregulated at host temperature. So far, ECA has been thoroughly analyzed at the structural and genetic level, however, its significance in vivo has been investigated in relatively few studies. ECA has been linked to pathogenesis in several species of bacteria, although this function seems to differ between the species. ECA has been shown to be involved in the flagellar assembly and motility in Serratia marcescens Also, the ECA-negative mutants of Salmonella enterica serovar Typhimurium proved to be significantly less virulent than the parental strain. ECA as a marker of Enterobacteriaceae family is a valuable indicator of water and food contaminations with enteric bacteria.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84920903555,

title = {Serological characterization of the enterobacterial common antigen substitution of the lipopolysaccharide of Yersinia enterocolitica o:3},

author = { M. Noszczyńska and K. Kasperkiewicz and K.A. Duda and J. Podhorodecka and K. Rabsztyn and K. Gwizdała and A.S. Świerzko and J. Radziejewska-Lebrecht and O. Holst and M. Skurnik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84920903555&doi=10.1099%2fmic.0.083493-0&partnerID=40&md5=e7ef363563f740b94a88bdf3f604463b},

doi = {10.1099/mic.0.083493-0},

issn = {13500872},

year  = {2015},

date = {2015-01-01},

journal = {Microbiology (United Kingdom)},

volume = {161},

number = {1},

pages = {219-227},

publisher = {Society for General Microbiology},

abstract = {Enterobacterial common antigen (ECA) is a polysaccharide present in all members of Enterobacteriaceae anchored either via phosphatidylglycerol (PG) or LPS to the outer leaflet of the outer membrane (ECAPG and ECALPS; respectively). Only the latter form is ECAimmunogenic. We previously demonstrated that Yersinia enterocolitica O: 3 and its rough (Ospecific polysaccharide-negative) mutants were ECA-immunogenic, suggesting that they contained ECALPS; however, it was not known which part of the LPS core region was involved in ECA binding. To address this, we used a set of three deep-rough LPS mutants for rabbit immunization. The polyvalent antisera obtained were: (i) analysed for the presence of anti-LPS and anti-ECA antibodies; (ii) treated with caprylic acid (CA) to precipitate IgM antibodies and protein aggregates; and (iii) adsorbed with live ECA-negative bacteria to obtain specific anti-ECA antisera. We demonstrated the presence of antibodies specific for both ECAPG and ECALPS in all antisera obtained. Both CA treatment and adsorption with ECA-negative bacteria efficiently removed anti-LPS antibodies, resulting in specific anti-ECA sera. The LPS of the ECALPS-positive deepest-rough mutant contained only lipid A and 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residues of the inner core, suggesting that ECALPS was linked to the Kdo region of LPS in Y. enterocolitica O:3. © 2015 The Authors.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}