@article{2-s2.0-85212699915,

title = {Changes in Ibuprofen Toxicity and Degradation in Response to Immobilization of Bacillus thuringiensis B1(2015b)},

author = { A. Marchlewicz and A. Dzionek and D. Wojcieszyńska and J. Borgulat and Ł. Jałowiecki and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85212699915&doi=10.3390%2fmolecules29235680&partnerID=40&md5=0f4f66895534812ea8976a6bd73fd857},

doi = {10.3390/molecules29235680},

year  = {2024},

date = {2024-01-01},

journal = {Molecules},

volume = {29},

number = {23},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {Ibuprofen is one of the most commonly used anti-inflammatory drugs by humans, resulting in its appearance in the environment, which can negatively affect organisms living in it. The studies undertaken have shown that the immobilized Bacillus thuringiensis B1(2015b) strain can decompose this drug at a rate of qmax = 0.36 mg/L*h, with a Ks constant of 0.95 mg/L for this process. An analysis of the effect of ibuprofen on the metabolic profile of the immobilized strain B1(2015b) showed an increase in the consumption of carbon, nitrogen, phosphorus, and sulfur compounds by this strain compared to the free strain. Studies on the toxicity of ibuprofen against the B1(2015b) strain indicated a small protective effect of the carrier, manifested by a slightly higher EC50 value = 1190 mg/L (for the free strain EC50 = 1175 mg/L). A toxicity analysis of intermedia formed during ibuprofen degradation indicated that the increase in toxicity is positively correlated with the degree of hydroxylation of ibuprofen metabolites. A toxicity analysis of the post-culture fluid obtained after ibuprofen degradation by the immobilized and free strain indicated that the products formed due to this process are completely safe. © 2024 by the authors.},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85202579479,

title = {Resource recovery and treatment of wastewaters using filamentous fungi},

author = { T. Sar and A. Marchlewicz and S. Harirchi and F.T. Mantzouridou and M. Işleten-Hoşoǧlu and M.Y. Akbas and C. Hellwig and M.J. Taherzadeh},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85202579479&doi=10.1016%2fj.scitotenv.2024.175752&partnerID=40&md5=e8c10afdedd77f0519d8c8b86cd4cd45},

doi = {10.1016/j.scitotenv.2024.175752},

year  = {2024},

date = {2024-01-01},

journal = {Science of the Total Environment},

volume = {951},

publisher = {Elsevier B.V.},

abstract = {Industrial wastewater, often characterized by its proximity to neutral pH, presents a promising opportunity for fungal utilization despite the prevalent preference of fungi for acidic conditions. This review addresses this discrepancy, highlighting the potential of certain industrial wastewaters, particularly those with low pH levels, for fungal biorefinery. Additionally, the economic implications of biomass recovery and compound separation, factors that require explicit were emphasized. Through an in-depth analysis of various industrial sectors, including food processing, textiles, pharmaceuticals, and paper-pulp, this study explores how filamentous fungi can effectively harness the nutrient-rich content of wastewaters to produce valuable resources. The pivotal role of ligninolytic enzymes synthesized by fungi in wastewater purification is examined, as well as their ability to absorb metal contaminants. Furthermore, the diverse benefits of fungal biorefinery are underscored, including the production of protein-rich single-cell protein, biolipids, enzymes, and organic acids, which not only enhance environmental sustainability but also foster economic growth. Finally, the challenges associated with scaling up fungal biorefinery processes for wastewater treatment are critically evaluated, providing valuable insights for future research and industrial implementation. This comprehensive analysis aims to elucidate the potential of fungal biorefinery in addressing industrial wastewater challenges while promoting sustainable resource utilization. © 2024 The Authors},

note = {0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85181777064,

title = {Naproxen as environmental pollution, its effect on bacteria metabolism and degradation mechanism in immobilized Planococcus sp. S5},

author = { A. Dzionek and D. Wojcieszyńska and A. Marchlewicz and W. Smułek and I.W. Potocka and Ł. Jałowiecki and J. Borgulat and G.A. Płaza and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85181777064&doi=10.1016%2fj.cej.2023.148174&partnerID=40&md5=899216654a60c6867293bebad6864093},

doi = {10.1016/j.cej.2023.148174},

year  = {2024},

date = {2024-01-01},

journal = {Chemical Engineering Journal},

volume = {481},

publisher = {Elsevier B.V.},

abstract = {Planococcus sp. S5 belongs to strains that degrade naproxen, one of the most popular non-steroidal anti-inflammatory drugs. In the presented work, the immobilization of the S5 strain was carried out on the Loofah plant sponge, which improved the degradation efficiency, and kinetic studies indicated the abolition of the inhibition by the substrate observed in the free cell system. At the same time, after immobilization, evident changes were observed in the metabolic profile of the strain, which was related to the specific microenvironment of the carrier. The study also presents the naproxen degradation pathway in a system with the immobilized S5 strain for the first time. The analysis of intermediates formed during the decomposition of naproxen indicated that this decomposition occurs through naphthalene and salicylic acid. Furthermore, the degradation of naproxen via 3-hydroxybenzoic acid to gentisic acid is also possible. The high efficiency of naproxen degradation by the immobilised S5 strain enables its use in bioremediation. © 2023 Elsevier B.V.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85149843765,

title = {Evaluation of the Defined Bacterial Consortium Efficacy in the Biodegradation of NSAIDs},

author = { A. Marchlewicz and U. Guzik and K.T. Hupert-Kocurek and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149843765&doi=10.3390%2fmolecules28052185&partnerID=40&md5=4092d66ed33e1bc7f11ea9018ced134c},

doi = {10.3390/molecules28052185},

issn = {14203049},

year  = {2023},

date = {2023-01-01},

journal = {Molecules},

volume = {28},

number = {5},

publisher = {MDPI},

abstract = {Due to the increasing pollution of wastewater with non-steroidal anti-inflammatory drugs, preparations need to be developed to decompose these drugs. This work aimed to develop a bacterial consortium with a defined composition and boundary conditions for the degradation of paracetamol and selected non-steroidal anti-inflammatory drugs (NSAIDs), including ibuprofen, naproxen, and diclofenac. The defined bacterial consortium consisted of Bacillus thuringiensis B1(2015b) and Pseudomonas moorei KB4 strains in a ratio of 1:2. During the tests, it was shown that the bacterial consortium worked in the pH range from 5.5 to 9 and temperatures of 15–35 °C, and its great advantage was its resistance to toxic compounds present in sewage, such as organic solvents, phenols, and metal ions. The degradation tests showed that, in the presence of the defined bacterial consortium in the sequencing batch reactor (SBR), drug degradation occurred at rates of 4.88, 10, 0.1, and 0.05 mg/day for ibuprofen, paracetamol, naproxen, and diclofenac, respectively. In addition, the presence of the tested strains was demonstrated during the experiment as well as after its completion. Therefore, the advantage of the described bacterial consortium is its resistance to the antagonistic effects of the activated sludge microbiome, which will enable it to be tested in real activated sludge conditions. © 2023 by the authors.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85138690790,

title = {Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation},

author = { D. Wojcieszyńska and J. Klamka and A. Marchlewicz and I.W. Potocka and J. Żur and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138690790&doi=10.3390%2fmolecules27185795&partnerID=40&md5=2dda4f9763acf86cf0ed785d52ea3576},

doi = {10.3390/molecules27185795},

issn = {14203049},

year  = {2022},

date = {2022-01-01},

journal = {Molecules},

volume = {27},

number = {18},

publisher = {MDPI},

abstract = {Immobilization is a commonly used method in response to the need to increase the resistance of microorganisms to the toxic effects of xenobiotics. In this study, a plant sponge from Luffa cylindrica was used as a carrier for the immobilization of the Stenotrophomonas maltophilia KB2 strain since such a carrier meets the criteria for high-quality carriers, i.e., low price and biodegradability. The optimal immobilization conditions were established as a temperature of 30 °C, pH 7.2, incubation time of 72 h, and an optical density of the culture of 1.4. The strain immobilized in such conditions was used for the biodegradation of naproxen, and an average rate of degradation of 3.8 µg/hour was obtained under cometabolic conditions with glucose. The obtained results indicate that a microbiological preparation based on immobilized cells on a luffa sponge can be used in bioremediation processes where it is necessary to remove the introduced carrier. © 2022 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85092155502,

title = {Degradation of diclofenac by new bacterial strains and its influence on the physiological status of cells},

author = { J. Żur and A. Marchlewicz and A. Piński and U. Guzik and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092155502&doi=10.1016%2fj.jhazmat.2020.124000&partnerID=40&md5=ea0a1291c85c273eacde445aa45e1c1c},

doi = {10.1016/j.jhazmat.2020.124000},

issn = {03043894},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Hazardous Materials},

volume = {403},

publisher = {Elsevier B.V.},

abstract = {Diclofenac (DCF) is one of the most commonly utilized non-steroidal anti-inflammatory drugs (NSAIDs), which is known to pose an ecotoxicological threat. In this study, from activated sludge and contaminated soil, we isolated four new bacterial strains able to degrade DCF under mono-substrate and co-metabolic conditions with glucose supplementation. We found that the effectiveness of DCF removal is strictly strain-specific and the addition of the primary substrate is not always beneficial. To assess the multidirectional influence of DCF on bacterial cells we evaluated the alterations of increasing concentrations of this drug on membrane structure. A significant increase was observed in the content of 17:0 cyclo fatty acid, which is responsible for reduced fluidity and profound changes in membrane rigidity. The cell injury and oxidative stress were assessed with biomarkers used as endpoints of toxicity, i.e. catalase (CAT), superoxide dismutase (SOD), lipids peroxidation (LPX), and both intra- and extracellular alkaline and acid phosphatase activity. Results indicated that DCF induced oxidative stress, frequently intensified by the addition of glucose. However, the response of the microbial cells to the presence of DCF should not be generalized, since the overall picture of the particular alterations greatly varied for each of the examined strains. © 2020 Elsevier B.V.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85092520348,

title = {Suitability of immobilized systems for microbiological degradation of endocrine disrupting compounds},

author = { D. Wojcieszyńska and A. Marchlewicz and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092520348&doi=10.3390%2fmolecules25194473&partnerID=40&md5=11c5639727e4fcd7a256dee3f126b652},

doi = {10.3390/molecules25194473},

issn = {14203049},

year  = {2020},

date = {2020-01-01},

journal = {Molecules},

volume = {25},

number = {19},

publisher = {MDPI AG},

abstract = {The rising pollution of the environment with endocrine disrupting compounds has increased interest in searching for new, e_ective bioremediation methods. Particular attention is paid to the search for microorganisms with high degradation potential and the possibility of their use in the degradation of endocrine disrupting compounds. Increasingly, immobilized microorganisms or enzymes are used in biodegradation systems. This review presents the main sources of endocrine disrupting compounds and identifies the risks associated with their presence in the environment. The main pathways of degradation of these compounds by microorganisms are also presented. The last part is devoted to an overview of the immobilization methods used for the purposes of enabling the use of biocatalysts in environmental bioremediation. © 2020 by the authors.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85070231029,

title = {Diversity and function of the microbial community under strong selective pressure of rotifers},

author = { E. Fiałkowska and B. Klimek and A. Marchlewicz and W. Kocerba-Soroka and J. Starzycka and A. Walczyńska and A. Pajdak-Stós},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070231029&doi=10.1002%2fjobm.201900167&partnerID=40&md5=2dd9f577364da4483b291c710e3d839e},

doi = {10.1002/jobm.201900167},

issn = {0233111X},

year  = {2019},

date = {2019-01-01},

journal = {Journal of Basic Microbiology},

volume = {59},

number = {8},

pages = {775-783},

publisher = {Wiley-VCH Verlag},

abstract = {We unearthed some interesting microecological discoveries while selecting for the most beneficial bacterial strains to be used as probiotics in Lecane inermis rotifer mass culture. For 3 years, we maintained the cultures of L. inermis, with selection for the highest growth rate and resistance to potential contamination. Then, we conducted further selection and isolation in two groups: rotifers inoculated with the bacterial consortium isolated from the rotifer cultures, and rotifers fed with a commercial bioproduct. Selection was conducted in demanding conditions, with particulate matter suspended in spring water as a substrate, without aeration and under strong consumer pressure, and led to selection of two cultivable strains isolated from the optimal rotifers culture. According to molecular analysis, these strains were Aeromonas veronii and Pseudomonas mosselii. Biolog® ECO plate tests showed that both investigated bacterial communities metabolized wide but similar range of substrates. Therefore, intensely selective conditions led to considerable reduction in bacterial community regarding taxonomy, but not in metabolic activity, showing a functional composition decoupling. Aside from this result, our novel selection method dedicated to the sustainable culture of two trophic levels, a directed selection procedure (DSC), could potentially lead to the development of biotechnologically valuable strains with high metabolic activity and the ability to metabolize different sorts of substrate without harmful impact on higher trophic levels. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85048021599,

title = {Paracetamol – toxicity and microbial utilization. Pseudomonas moorei KB4 as a case study for exploring degradation pathway},

author = { J. Żur and D. Wojcieszyńska and K.T. Hupert-Kocurek and A. Marchlewicz and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048021599&doi=10.1016%2fj.chemosphere.2018.04.179&partnerID=40&md5=9941e36fe7554a810b10735fb721bbcc},

doi = {10.1016/j.chemosphere.2018.04.179},

issn = {00456535},

year  = {2018},

date = {2018-01-01},

journal = {Chemosphere},

volume = {206},

pages = {192-202},

publisher = {Elsevier Ltd},

abstract = {Paracetamol, a widely used analgesic and antipyretic drug, is currently one of the most emerging pollutants worldwide. Besides its wide prevalence in the literature only several bacterial strains able to degrade this compound have been described. In this study, we isolated six new bacterial strains able to remove paracetamol. The isolated strains were identified as the members of Pseudomonas, Bacillus, Acinetobacter and Sphingomonas genera and characterized phenotypically and biochemically using standard methods. From the isolated strains, Pseudomonas moorei KB4 was able to utilize 50 mg L−1 of paracetamol. As the main degradation products, p-aminophenol and hydroquinone were identified. Based on the measurements of specific activity of acyl amidohydrolase, deaminase and hydroquinone 1,2-dioxygenase and the results of liquid chromatography analyses, we proposed a mechanism of paracetamol degradation by KB4 strain under co-metabolic conditions with glucose. Additionally, toxicity bioassays and the influence of various environmental factors, including pH, temperature, heavy metals at no-observed-effective-concentrations, and the presence of aromatic compounds on the efficiency and mechanism of paracetamol degradation by KB4 strain were determined. This comprehensive study about paracetamol biodegradation will be helpful in designing a treatment systems of wastewaters contaminated with paracetamol. © 2018 Elsevier Ltd},

note = {63},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85048822480,

title = {Organic micropollutants paracetamol and ibuprofen—toxicity, biodegradation, and genetic background of their utilization by bacteria},

author = { J. Żur and A. Piński and A. Marchlewicz and K.T. Hupert-Kocurek and D. Wojcieszyńska and U. Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048822480&doi=10.1007%2fs11356-018-2517-x&partnerID=40&md5=bed6633cc8ff497e87e086da67cbead2},

doi = {10.1007/s11356-018-2517-x},

issn = {09441344},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Science and Pollution Research},

volume = {25},

number = {22},

pages = {21498-21524},

publisher = {Springer Verlag},

abstract = {Currently, analgesics and nonsteroidal anti-inflammatory drugs (NSAIDs) are classified as one of the most emerging group of xenobiotics and have been detected in various natural matrices. Among them, monocyclic paracetamol and ibuprofen, widely used to treat mild and moderate pain are the most popular. Since long-term adverse effects of these xenobiotics and their biological and pharmacokinetic activity especially at environmentally relevant concentrations are better understood, degradation of such contaminants has become a major concern. Moreover, to date, conventional wastewater treatment plants (WWTPs) are not fully adapted to remove that kind of micropollutants. Bioremediation processes, which utilize bacterial strains with increased degradation abilities, seem to be a promising alternative to the chemical methods used so far. Nevertheless, despite the wide prevalence of paracetamol and ibuprofen in the environment, toxicity and mechanism of their microbial degradation as well as genetic background of these processes remain not fully characterized. In this review, we described the current state of knowledge about toxicity and biodegradation mechanisms of paracetamol and ibuprofen and provided bioinformatics analysis concerning the genetic bases of these xenobiotics decomposition. © 2018, The Author(s).},

note = {90},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85032711058,

title = {Exploring the degradation of ibuprofen by bacillus thuringiensis B1(2015b): The new pathway and factors affecting degradation},

author = { A. Marchlewicz and U. Guzik and W. Smułek and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032711058&doi=10.3390%2fmolecules22101676&partnerID=40&md5=6dfc7f57f372853f7a5a1e14f2c71368},

doi = {10.3390/molecules22101676},

issn = {14203049},

year  = {2017},

date = {2017-01-01},

journal = {Molecules},

volume = {22},

number = {10},

publisher = {MDPI AG},

abstract = {Ibuprofen is one of the most often detected pollutants in the environment, particularly at landfill sites and in wastewaters. Contamination with pharmaceuticals is often accompanied by the presence of other compounds which may influence their degradation. This work describes the new degradation pathway of ibuprofen by Bacillus thuringiensis B1(2015b), focusing on enzymes engaged in this process. It is known that the key intermediate which transformation limits the velocity of the degradation process is hydroxyibuprofen. As the degradation rate also depends on various factors, the influence of selected heavy metals and aromatic compounds on ibuprofen degradation by the B1(2015b) strain was examined. Based on the values of non-observed effect concentration (NOEC) it was found that the toxicity of tested metals increases from Hg(II) < Cu(II) < Cd(II) < Co(II) < Cr(VI). Despite the toxic effect of metals, the biodegradation of ibuprofen was observed. The addition of Co2+ ions into the medium significantly extended the time necessary for the complete removal of ibuprofen. It was shown that Bacillus thuringiensis B1(2015b) was able to degrade ibuprofen in the presence of phenol, benzoate, and 2-chlorophenol. Moreover, along with the removal of ibuprofen, degradation of phenol and benzoate was observed. Introduction of 4-chlorophenol into the culture completely inhibits degradation of ibuprofen. © 2017 by The Authors. Licensee MDPI, Basel, Switzerland.},

note = {31},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85020934837,

title = {Dynamics of ibuprofen biodegradation by Bacillus sp. B1(2015b)},

author = { A. Marchlewicz and U. Guzik and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020934837&doi=10.1515%2faep-2017-0020&partnerID=40&md5=df1595f516679cc4115587d01a52aa2e},

doi = {10.1515/aep-2017-0020},

issn = {03248461},

year  = {2017},

date = {2017-01-01},

journal = {Archives of Environmental Protection},

volume = {43},

number = {2},

pages = {60-64},

publisher = {De Gruyter Open Ltd},

abstract = {High intake of over-the-counter, non-steroidal anti-inflammatory drugs, such as ibuprofen, has resulted in their presence in wastewaters and surface waters. The potentially harmful effect of ibuprofen present in the waters has led to a search for new methods of drugs' removal from the environment. One of the most important technological and economical solutions comprises microbiological degradation of these resistant pollutants. Searching for new strains able to degrade ibuprofen could be one of the answers for increasing the detection of pharmaceuticals in the waters. In this study, the ability of bacterial strain Bacillus thuringiensis B1(2015b) to remove ibuprofen is described. Bacteria were cultured in both monosubstrate and cometabolic systems with 1, 3, 5, 7 and 9 mg L-1 ibuprofen and 1 g L-1 glucose as a carbon source. Bacillus thuringiensis B1(2015b) removed ibuprofen up to 9 mg L-1 in 232 hours in the monosubstrate culture, whereas in the cometabolic culture the removal of the drug was over 6 times faster. That is why the examined strain could be used to enhance the bioremediation of ibuprofen. © Archives of Environmental Protection 2017.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85010723117,

title = {Toxicity and biodegradation of ibuprofen by Bacillus thuringiensis B1(2015b)},

author = { A. Marchlewicz and U. Guzik and K.T. Hupert-Kocurek and A. Nowak and S. Wilczyńska and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010723117&doi=10.1007%2fs11356-017-8372-3&partnerID=40&md5=f8a6724832ad5bb834800f9207067ea7},

doi = {10.1007/s11356-017-8372-3},

issn = {09441344},

year  = {2017},

date = {2017-01-01},

journal = {Environmental Science and Pollution Research},

volume = {24},

number = {8},

pages = {7572-7584},

publisher = {Springer Verlag},

abstract = {In recent years, the increased intake of ibuprofen has resulted in the presence of the drug in the environment. This work presents results of a study on degradation of ibuprofen at 25 mg L−1 in the presence of glucose, as an additional carbon source by Bacillus thuringiensis B1(2015b). In the cometabolic system, the maximum specific growth rate of the bacterial strain was 0.07 ± 0.01 mg mL−1 h−1 and Ksμ 0.27 ± 0.15 mg L−1. The maximum specific ibuprofen removal rate and the value of the half-saturation constant were qmax = 0.24 ± 0.02 mg mL−1 h−1 and Ks = 2.12 ± 0.56 mg L−1, respectively. It has been suggested that monooxygenase and catechol 1,2-dioxygenase are involved in ibuprofen degradation by B. thuringiensis B1(2015b). Toxicity studies showed that B. thuringiensis B1(2015b) is more resistant to ibuprofen than other tested organisms. The EC50 of ibuprofen on the B1 strain is 809.3 mg L−1, and it is 1.5 times higher than the value of the microbial toxic concentration (MTCavg). The obtained results indicate that B. thuringiensis B1(2015b) could be a useful tool in biodegradation/bioremediation processes. © 2017, The Author(s).},

note = {37},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84971602981,

title = {Bacillus thuringiensis B1(2015b) is a Gram-Positive Bacteria Able to Degrade Naproxen and Ibuprofen},

author = { A. Marchlewicz and D. Domaradzka and U. Guzik and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84971602981&doi=10.1007%2fs11270-016-2893-0&partnerID=40&md5=ef678be69f18fb39e03d8138a3438e72},

doi = {10.1007/s11270-016-2893-0},

issn = {00496979},

year  = {2016},

date = {2016-01-01},

journal = {Water, Air, and Soil Pollution},

volume = {227},

number = {6},

publisher = {Springer International Publishing},

abstract = {A Gram-positive bacterium, designated as strain B1(2015b), was isolated from the soil of the chemical factory “Organika-Azot” in Jaworzno, Poland. On the basis of 16S rRNA gene sequence analysis, the isolated strain was classified as a Bacillus thuringiensis species. Strain B1(2015b) is able to degrade ibuprofen and naproxen, however, these compounds are not sufficient carbon sources for this strain. In the presence of glucose, Bacillus thuringiensis B1(2015b) degrades ibuprofen and naproxen with higher efficiency. Twenty milligrams per liter of ibuprofen was degraded within 6 days and 6 mg l−1 of naproxen was removed within 35 days. Simultaneously, the growth of the bacterial culture was observed. The obtained results suggest that Bacillus thuringiensis B1(2015b) appears to be a powerful and useful tool in the bioremediation of non-steroidal anti-inflammatory drugs-contaminated environment. © 2016, The Author(s).},

note = {68},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84942905402,

title = {Over-the-Counter Monocyclic Non-Steroidal Anti-Inflammatory Drugs in Environment - Sources, Risks, Biodegradation},

author = { A. Marchlewicz and U. Guzik and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942905402&doi=10.1007%2fs11270-015-2622-0&partnerID=40&md5=6929a3f705a50f9a8f11507a5a6bcdff},

doi = {10.1007/s11270-015-2622-0},

issn = {00496979},

year  = {2015},

date = {2015-01-01},

journal = {Water, Air, and Soil Pollution},

volume = {226},

number = {10},

publisher = {Kluwer Academic Publishers},

abstract = {Recently, the increased use of monocyclic non-steroidal anti-inflammatory drugs has resulted in their presence in the environment. This may have potential negative effects on living organisms. The biotransformation mechanisms of monocyclic non-steroidal anti-inflammatory drugs in the human body and in other mammals occur by hydroxylation and conjugation with glycine or glucuronic acid. Biotransformation/biodegradation of monocyclic non-steroidal anti-inflammatory drugs in the environment may be caused by fungal or bacterial microorganisms. Salicylic acid derivatives are degraded by catechol or gentisate as intermediates which are cleaved by dioxygenases. The key intermediate of the paracetamol degradation pathways is hydroquinone. Sometimes, after hydrolysis of this drug, 4-aminophenol is formed, which is a dead-end metabolite. Ibuprofen is metabolized by hydroxylation or activation with CoA, resulting in the formation of isobutylocatechol. The aim of this work is to attempt to summarize the knowledge about environmental risk connected with the presence of over-the-counter anti-inflammatory drugs, their sources and the biotransformation and/or biodegradation pathways of these drugs. © 2015 The Author(s).},

note = {19},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84937202609,

title = {Properties, occurrence and biodegradation of ibuprofen in aquatic environment [Włäciwöci, występowanie i biodegradacja ibuprofenu w ̈rodowisku wodnym]},

author = { A. Marchlewicz and U. Guzik and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937202609&partnerID=40&md5=d9667d774d8c187ab4d602e0aa4a3774},

issn = {12306169},

year  = {2015},

date = {2015-01-01},

journal = {Ochrona Srodowiska},

volume = {37},

number = {1},

pages = {65-70},

publisher = {Polskie Zrzeszenie Inzynierow},

abstract = {Common use of non-steroidal anti-inflammatory drugs (NSAIDs), including ibuprofen, leads to drug presence in sewage but also in surface waters, which they enter with municipal treatment plants effluent. As a result, the drugs may also be found in tap water. Due to low ibuprofen concentration in aquatic environment, acute toxicity is not observed. Yet, continuous exposure of aquatic organisms to the drug makes it important to study chronic toxicity mechanisms. Moreover, knowledge of ibuprofen migration and the time course of its biodegradation in the aquatic environment is incomplete. Only a few microorganism species (mainly fungi) able to metabolize ibuprofen have been described. The current research suggests that ibuprofen biotransformation proceeds by its hydroxylation to 1,2-dihydroxyibuprofen. Sphingomonas spp. Ibu-2 is the only described bacterial strain able to use ibuprofen as a sole carbon and energy source. Thioestrification is the first step in ibuprofen degradation. Then, propionic chain is removed with simultaneous oxidation of aromatic ring to 4-isobutylcatechol, which is then cleaved by extradiol enzymes. Knowledge of pathways of NSAID metabolism will allow for more effective removal of such pollutants from municipal wastewater, resulting in a significant improvement of surface water quality.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84896328434,

title = {Enhancement of biodegradation potential of catechol 1,2-dioxygenase through its immobilization in calcium alginate gel},

author = { U. Guzik and K.T. Hupert-Kocurek and A. Marchlewicz and D. Wojcieszyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896328434&doi=10.1016%2fj.ejbt.2014.02.001&partnerID=40&md5=bc1dbc1301015a0aa17997acb14c24e0},

doi = {10.1016/j.ejbt.2014.02.001},

issn = {07173458},

year  = {2014},

date = {2014-01-01},

journal = {Electronic Journal of Biotechnology},

volume = {17},

number = {2},

pages = {83-88},

publisher = {Electronic Journal of Biotechnology},

abstract = {Background:In biodegradation processes free enzymes often undergo deactivation. Thus, it is very important to obtain highly stable enzymes by different methods. Immobilization allows for successful stabilization of many multimeric enzymes by increasing the rigidity of the enzyme structure. This study aimed to evaluate some environmental factors that affect catechol 1,2-dioxygenase from Stenotrophomonas maltophilia KB2 immobilized in alginate hydrogel. The goal of the present work was to improve the functional stability of the enzyme by increas-ing its structural rigidity.Results:Immobilization yield and expressed activity were 100% and 56%, respectively. Under the same storage conditions, the activity of the immobilized enzyme was still observed on the 28th day of incubation at 4 °C, whereas the free enzyme lost its activity after 14 days. The immobilized enzyme required approximately 10 °C lower temperature for its optimal activity than the free enzyme. Immobilization shifted the optimal pH from 8 for the soluble enzyme to 7 for the immobilized enzyme. The immobilized catechol 1,2-dioxygenase showed ac-tivity against 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol, and 3,5-dichlorocatechol. The immobilization of the enzyme promoted its stabilization against any distorting agents:aliphatic alcohols, phenols, and chelators.Conclusions:The entrapment of the catechol 1,2-dioxygenase from S. maltophilia KB2 has been shown to be an effective method for improving the functional properties of the enzyme. Increased resistance to inactivation by higher substrate concentration and other factors affecting enzyme activity as well as broadened substrate spec-ificity compared to the soluble enzyme. makes the immobilized catechol 1,2-dioxygenase suitable for the biore-mediation and detoxification of xenobiotic-contaminated environments. © 2014 Pontificia Universidad Católica de Valparaiso. Production and hosting by Elsevier B.V.All rights reserved.},

note = {38},

keywords = {},

pubstate = {published},

tppubtype = {article}

}