• dr Zbigniew Burdach
Stanowisko: St.specj.b-techn.
Jednostka: Wydział Nauk Przyrodniczych
Adres: 40-032 Katowice, ul. Jagiellońska 28
Piętro: II
Numer pokoju: A-235
Telefon: (32) 2009 466
E-mail: zbigniew.burdach@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 6505764676
Publikacje z bazy Scopus
2024
Karcz, W.; Burdach, Z.
In: International Journal of Molecular Sciences, vol. 25, no. 19, 2024, ISSN: 16616596.
@article{2-s2.0-85206475807,
title = {The Complexity of the Influence of Growth Substances, Heavy Metals, and Their Combination on the Volume Dynamics of Vacuoles Isolated from Red Beet (Beta vulgaris L.) Taproot Cells},
author = { W. Karcz and Z. Burdach},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85206475807&doi=10.3390%2fijms251910842&partnerID=40&md5=c849c260e663b3b5d6ee9f63e0cd4663},
doi = {10.3390/ijms251910842},
issn = {16616596},
year = {2024},
date = {2024-01-01},
journal = {International Journal of Molecular Sciences},
volume = {25},
number = {19},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {The plant vacuole is a very dynamic organelle that can occupy more than 90% of the cell volume and is essential to plant cell growth and development, the processes in which auxin (indole-3-acetic acid; IAA) is a central player. It was found that when IAA or FC (fusicoccin) was present in the control medium of vacuoles isolated from red beet taproots at a final concentration of 1 µM, it increased their volume to a level that was 26% or 36% higher than that observed in the control medium without growth regulators, respectively. In the presence of IAA and FC, the time after which most vacuoles ruptured was about 10 min longer for IAA than for FC. However, when cadmium (Cd) or lead (Pb) was present in the control medium at a final concentration of 100 µM, it increased the volume of the vacuoles by about 26% or 80% compared to the control, respectively. The time after which the vacuoles ruptured was similar for both metals. The combined effect of IAA and Pb on the volume of the vacuoles was comparable with that observed in the presence of Pb only, while for FC combined with Pb, it was additive. The use of IAA or FC together with Cd caused in both cases a decrease in the vacuole volumes by about 50%. The data presented in this study are discussed, taking into account the structure and function of the vacuolar membrane (tonoplast) and their changes in the presence of growth substances, heavy metals, and their combination. © 2024 by the authors.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The plant vacuole is a very dynamic organelle that can occupy more than 90% of the cell volume and is essential to plant cell growth and development, the processes in which auxin (indole-3-acetic acid; IAA) is a central player. It was found that when IAA or FC (fusicoccin) was present in the control medium of vacuoles isolated from red beet taproots at a final concentration of 1 µM, it increased their volume to a level that was 26% or 36% higher than that observed in the control medium without growth regulators, respectively. In the presence of IAA and FC, the time after which most vacuoles ruptured was about 10 min longer for IAA than for FC. However, when cadmium (Cd) or lead (Pb) was present in the control medium at a final concentration of 100 µM, it increased the volume of the vacuoles by about 26% or 80% compared to the control, respectively. The time after which the vacuoles ruptured was similar for both metals. The combined effect of IAA and Pb on the volume of the vacuoles was comparable with that observed in the presence of Pb only, while for FC combined with Pb, it was additive. The use of IAA or FC together with Cd caused in both cases a decrease in the vacuole volumes by about 50%. The data presented in this study are discussed, taking into account the structure and function of the vacuolar membrane (tonoplast) and their changes in the presence of growth substances, heavy metals, and their combination. © 2024 by the authors.
2023
Miśkiewicz, J.; Burdach, Z.; Trela, Z.; Siemieniuk, A.; Karcz, W.
Multifractal Analysis of the Influence of Indole-3-Acetic Acid on Fast-Activating Vacuolar (FV) Channels of Beta vulgaris L. Taproot Cells Journal Article
In: Membranes, vol. 13, no. 4, 2023, ISSN: 20770375.
@article{2-s2.0-85153846939,
title = {Multifractal Analysis of the Influence of Indole-3-Acetic Acid on Fast-Activating Vacuolar (FV) Channels of Beta vulgaris L. Taproot Cells},
author = { J. Miśkiewicz and Z. Burdach and Z. Trela and A. Siemieniuk and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85153846939&doi=10.3390%2fmembranes13040406&partnerID=40&md5=82d64bccdd33b677f6cce9e8792098fe},
doi = {10.3390/membranes13040406},
issn = {20770375},
year = {2023},
date = {2023-01-01},
journal = {Membranes},
volume = {13},
number = {4},
publisher = {MDPI},
abstract = {In this paper, the multifractal properties of the ion current time series in the fast-activating vacuolar (FV) channels of Beta vulgaris L. taproot cells were investigated. These channels are permeable for only monovalent cations and mediate K+ at very low concentrations of cytosolic Ca2+ and large voltages of either polarity. Using the patch clamp technique, the currents of the FV channels in red beet taproot vacuoles were recorded and analysed by using the multifractal detrended fluctuation analysis (MFDFA) method. The activity of the FV channels depended on the external potential and was sensitive to the auxin. It was also shown that the singularity spectrum of the ion current in the FV channels is non-singular, and the multifractal parameters, i.e., the generalised Hurst exponent and the singularity spectrum, were modified in the presence of IAA. Taking into account the obtained results, it can be suggested that the multifractal properties of fast-activating vacuolar (FV) K+ channels, indicating the existence of long-term memory, should be taken into account in the molecular mechanism of the auxin-induced growth of plant cells. © 2023 by the authors.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this paper, the multifractal properties of the ion current time series in the fast-activating vacuolar (FV) channels of Beta vulgaris L. taproot cells were investigated. These channels are permeable for only monovalent cations and mediate K+ at very low concentrations of cytosolic Ca2+ and large voltages of either polarity. Using the patch clamp technique, the currents of the FV channels in red beet taproot vacuoles were recorded and analysed by using the multifractal detrended fluctuation analysis (MFDFA) method. The activity of the FV channels depended on the external potential and was sensitive to the auxin. It was also shown that the singularity spectrum of the ion current in the FV channels is non-singular, and the multifractal parameters, i.e., the generalised Hurst exponent and the singularity spectrum, were modified in the presence of IAA. Taking into account the obtained results, it can be suggested that the multifractal properties of fast-activating vacuolar (FV) K+ channels, indicating the existence of long-term memory, should be taken into account in the molecular mechanism of the auxin-induced growth of plant cells. © 2023 by the authors.
Karcz, W.; Burdach, Z.; Rudnicka, M.
In: Plants, vol. 12, no. 4, 2023, ISSN: 22237747, (1).
@article{2-s2.0-85149128761,
title = {The Effects of 1,4-Naphthoquinone (NQ) and Naphthazarin (5,8-Dihydroxy-1,4-naphthoquinone, DHNQ) Individually and in Combination on Growth and Oxidative Stress in Maize (Zea mays L.) Seedlings},
author = { W. Karcz and Z. Burdach and M. Rudnicka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149128761&doi=10.3390%2fplants12040900&partnerID=40&md5=ad6c9862904a9ec0e860c0b5e4293ac1},
doi = {10.3390/plants12040900},
issn = {22237747},
year = {2023},
date = {2023-01-01},
journal = {Plants},
volume = {12},
number = {4},
publisher = {MDPI},
abstract = {This study investigated the effects of 1,4-naphthoquinone (NQ) and naphthazarin (5;8-dihydroxy-1;4-naphthoquinone; DHNQ) individually and in combination, applied at low concentrations (0.1; 1; and 10 nM), on growth, hydrogen peroxide (H2O2) production, catalase activity, and lipid peroxidation in maize seedlings. It was found that NQ at 0.1 and 1 nM and DHNQ at 0.1 nM significantly stimulated the fresh weight of the aboveground parts of the seedlings (APS), while the fresh weight of the underground parts of the seedlings (UPS) was enhanced only at 0.1 nM NQ. Interestingly, DHNQ at higher concentrations (1 and 10 nM) significantly diminished the fresh weight of the APS and UPS. When NQ and DHNQ were applied together, an increase in the fresh weight of the APS at all of the concentrations studied was observed. It was also found that NQ and DHNQ individually and in combination, at all concentrations studied, decreased the H2O2 production in the aboveground and underground parts of maize seedlings. The presence of the DHNQ at higher concentrations (1 and 10 nM) triggered an increase in the catalase (CAT) activity of the UPS and APS compared to the control. However, NQ added at 1 nM decreased the CAT activity of both the UPS and APS, while 10 nM increased the CAT activity of UPS. NQ and DHNQ applied together at 0.1 and 10 nM almost completely inhibited catalase activity in the UPS and APS. The data that were obtained for lipid peroxidation, measured as the malondialdehyde (MDA) concentration, indicated that NQ and DHNQ at all concentrations studied decreased the MDA content of the UPS, while both naphthoquinones increased it in APS. The data presented here are discussed taking into account the mechanisms via which naphthoquinones interact with biological systems. © 2023 by the authors.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study investigated the effects of 1,4-naphthoquinone (NQ) and naphthazarin (5;8-dihydroxy-1;4-naphthoquinone; DHNQ) individually and in combination, applied at low concentrations (0.1; 1; and 10 nM), on growth, hydrogen peroxide (H2O2) production, catalase activity, and lipid peroxidation in maize seedlings. It was found that NQ at 0.1 and 1 nM and DHNQ at 0.1 nM significantly stimulated the fresh weight of the aboveground parts of the seedlings (APS), while the fresh weight of the underground parts of the seedlings (UPS) was enhanced only at 0.1 nM NQ. Interestingly, DHNQ at higher concentrations (1 and 10 nM) significantly diminished the fresh weight of the APS and UPS. When NQ and DHNQ were applied together, an increase in the fresh weight of the APS at all of the concentrations studied was observed. It was also found that NQ and DHNQ individually and in combination, at all concentrations studied, decreased the H2O2 production in the aboveground and underground parts of maize seedlings. The presence of the DHNQ at higher concentrations (1 and 10 nM) triggered an increase in the catalase (CAT) activity of the UPS and APS compared to the control. However, NQ added at 1 nM decreased the CAT activity of both the UPS and APS, while 10 nM increased the CAT activity of UPS. NQ and DHNQ applied together at 0.1 and 10 nM almost completely inhibited catalase activity in the UPS and APS. The data that were obtained for lipid peroxidation, measured as the malondialdehyde (MDA) concentration, indicated that NQ and DHNQ at all concentrations studied decreased the MDA content of the UPS, while both naphthoquinones increased it in APS. The data presented here are discussed taking into account the mechanisms via which naphthoquinones interact with biological systems. © 2023 by the authors.
2022
Karcz, W.; Burdach, Z.
In: BMC Plant Biology, vol. 22, no. 1, 2022, ISSN: 14712229.
@article{2-s2.0-85135264238,
title = {The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study},
author = { W. Karcz and Z. Burdach},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135264238&doi=10.1186%2fs12870-022-03778-4&partnerID=40&md5=9237dfe8d894a1e13df9f6fe76439d1f},
doi = {10.1186/s12870-022-03778-4},
issn = {14712229},
year = {2022},
date = {2022-01-01},
journal = {BMC Plant Biology},
volume = {22},
number = {1},
publisher = {BioMed Central Ltd},
abstract = {Background: In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results: It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root; mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA; indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion: It is suggested that a DC electric field inhibits the plasma membrane H+ pump activity and K+ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays K+ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. © 2022, The Author(s).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Background: In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results: It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root; mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA; indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion: It is suggested that a DC electric field inhibits the plasma membrane H+ pump activity and K+ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays K+ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. © 2022, The Author(s).
2021
Siemieniuk, A.; Burdach, Z.; Karcz, W.
In: International Journal of Molecular Sciences, vol. 22, no. 23, 2021, ISSN: 16616596.
@article{2-s2.0-85119610574,
title = {A comparison of the effect of lead (Pb) on the slow vacuolar (sv) and fast vacuolar (fv) channels in red beet (beta vulgaris l.) taproot vacuoles},
author = { A. Siemieniuk and Z. Burdach and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119610574&doi=10.3390%2fijms222312621&partnerID=40&md5=b9f8b810e216555eaed034f340681a77},
doi = {10.3390/ijms222312621},
issn = {16616596},
year = {2021},
date = {2021-01-01},
journal = {International Journal of Molecular Sciences},
volume = {22},
number = {23},
publisher = {MDPI},
abstract = {Little is known about the effect of lead on the activity of the vacuolar K+ channels. Here, the patch-clamp technique was used to compare the impact of lead (PbCl2) on the slow-activating (SV) and fast-activating (FV) vacuolar channels. It was revealed that, under symmetrical 100-mM K+, the macroscopic currents of the SV channels exhibited a typical slow activation and a strong outward rectification of the steady-state currents, while the macroscopic currents of the FV channels displayed instantaneous currents, which, at the positive potentials, were about three-fold greater compared to the one at the negative potentials. When PbCl2 was added to the bath solution at a final concentration of 100 µM, it decreased the macroscopic outward currents of both channels but did not change the inward currents. The single-channel recordings demonstrated that cytosolic lead causes this macroscopic effect by a decrease of the single-channel conductance and decreases the channel open probability. We propose that cytosolic lead reduces the current flowing through the SV and FV channels, which causes a decrease of the K+ fluxes from the cytosol to the vacuole. This finding may, at least in part, explain the mechanism by which cytosolic Pb2+ reduces the growth of plant cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Little is known about the effect of lead on the activity of the vacuolar K+ channels. Here, the patch-clamp technique was used to compare the impact of lead (PbCl2) on the slow-activating (SV) and fast-activating (FV) vacuolar channels. It was revealed that, under symmetrical 100-mM K+, the macroscopic currents of the SV channels exhibited a typical slow activation and a strong outward rectification of the steady-state currents, while the macroscopic currents of the FV channels displayed instantaneous currents, which, at the positive potentials, were about three-fold greater compared to the one at the negative potentials. When PbCl2 was added to the bath solution at a final concentration of 100 µM, it decreased the macroscopic outward currents of both channels but did not change the inward currents. The single-channel recordings demonstrated that cytosolic lead causes this macroscopic effect by a decrease of the single-channel conductance and decreases the channel open probability. We propose that cytosolic lead reduces the current flowing through the SV and FV channels, which causes a decrease of the K+ fluxes from the cytosol to the vacuole. This finding may, at least in part, explain the mechanism by which cytosolic Pb2+ reduces the growth of plant cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
2020
Burdach, Z.; Siemieniuk, A.; Karcz, W.
Effect of auxin (IAA) on the fast vacuolar (FV) channels in red beet (Beta vulgaris L.) taproot vacuoles Journal Article
In: International Journal of Molecular Sciences, vol. 21, no. 14, pp. 1-11, 2020, ISSN: 16616596, (5).
@article{2-s2.0-85087646572,
title = {Effect of auxin (IAA) on the fast vacuolar (FV) channels in red beet (Beta vulgaris L.) taproot vacuoles},
author = { Z. Burdach and A. Siemieniuk and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087646572&doi=10.3390%2fijms21144876&partnerID=40&md5=3d4cb4c0970cadbc3552dc5cac22e835},
doi = {10.3390/ijms21144876},
issn = {16616596},
year = {2020},
date = {2020-01-01},
journal = {International Journal of Molecular Sciences},
volume = {21},
number = {14},
pages = {1-11},
publisher = {MDPI AG},
abstract = {In contrast to the well-studied effect of auxin on the plasma membrane K+ channel activity, little is known about the role of this hormone in regulating the vacuolar K+ channels. Here, the patch-clamp technique was used to investigate the effect of auxin (IAA) on the fast-activating vacuolar (FV) channels. It was found that the macroscopic currents displayed instantaneous currents, which at the positive potentials were about three-fold greater compared to the one at the negative potentials. When auxin was added to the bath solution at a final concentration of 1 µM, it increased the outward currents by about 60%, but did not change the inward currents. The imposition of a ten-fold vacuole-to-cytosol KCl gradient stimulated the efflux of K+ from the vacuole into the cytosol and reduced the K+ current in the opposite direction. The addition of IAA to the bath solution with the 10/100 KCl gradient decreased the outward current and increased the inward current. Luminal auxin reduced both the outward and inward current by approximately 25% compared to the control. The single channel recordings demonstrated that cytosolic auxin changed the open probability of the FV channels at the positive voltages to a moderate extent, while it significantly increased the amplitudes of the single channel outward currents and the number of open channels. At the positive voltages, auxin did not change the unitary conductance of the single channels. We suggest that auxin regulates the activity of the fast-activating vacuolar (FV) channels, thereby causing changes of the K+ fluxes across the vacuolar membrane. This mechanism might serve to tightly adjust the volume of the vacuole during plant cell expansion. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In contrast to the well-studied effect of auxin on the plasma membrane K+ channel activity, little is known about the role of this hormone in regulating the vacuolar K+ channels. Here, the patch-clamp technique was used to investigate the effect of auxin (IAA) on the fast-activating vacuolar (FV) channels. It was found that the macroscopic currents displayed instantaneous currents, which at the positive potentials were about three-fold greater compared to the one at the negative potentials. When auxin was added to the bath solution at a final concentration of 1 µM, it increased the outward currents by about 60%, but did not change the inward currents. The imposition of a ten-fold vacuole-to-cytosol KCl gradient stimulated the efflux of K+ from the vacuole into the cytosol and reduced the K+ current in the opposite direction. The addition of IAA to the bath solution with the 10/100 KCl gradient decreased the outward current and increased the inward current. Luminal auxin reduced both the outward and inward current by approximately 25% compared to the control. The single channel recordings demonstrated that cytosolic auxin changed the open probability of the FV channels at the positive voltages to a moderate extent, while it significantly increased the amplitudes of the single channel outward currents and the number of open channels. At the positive voltages, auxin did not change the unitary conductance of the single channels. We suggest that auxin regulates the activity of the fast-activating vacuolar (FV) channels, thereby causing changes of the K+ fluxes across the vacuolar membrane. This mechanism might serve to tightly adjust the volume of the vacuole during plant cell expansion. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Miśkiewicz, J.; Trela, Z.; Burdach, Z.; Karcz, W.; Balińska-Miśkiewicz, W.
Long range correlations of the ion current in SV channels. Met3PbCl influence study Journal Article
In: PLoS ONE, vol. 15, no. 3, 2020, ISSN: 19326203, (4).
@article{2-s2.0-85080900098,
title = {Long range correlations of the ion current in SV channels. Met3PbCl influence study},
author = { J. Miśkiewicz and Z. Trela and Z. Burdach and W. Karcz and W. Balińska-Miśkiewicz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85080900098&doi=10.1371%2fjournal.pone.0229433&partnerID=40&md5=10fddf27191100d7c31abf9471d441d0},
doi = {10.1371/journal.pone.0229433},
issn = {19326203},
year = {2020},
date = {2020-01-01},
journal = {PLoS ONE},
volume = {15},
number = {3},
publisher = {Public Library of Science},
abstract = {The long-range correlations within the current signal time series of the Beta vulgaris vacuolar membrane under the influence of organolead compound (Met3PbCl) are investigated. The current time series is transformed into a dwell time series. Then the rescaled range and detrended fluctuations analyses are used. It is shown that the presence of Met3PbCl in the solution decreases the mean value of the Hurst exponent and therefore influences the longrange correlations in ionic channel current. This observation is statistically significant. An ion channel model is built and the experimental results reconstructed and analysed. © 2020 Miśkiewicz et al.},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The long-range correlations within the current signal time series of the Beta vulgaris vacuolar membrane under the influence of organolead compound (Met3PbCl) are investigated. The current time series is transformed into a dwell time series. Then the rescaled range and detrended fluctuations analyses are used. It is shown that the presence of Met3PbCl in the solution decreases the mean value of the Hurst exponent and therefore influences the longrange correlations in ionic channel current. This observation is statistically significant. An ion channel model is built and the experimental results reconstructed and analysed. © 2020 Miśkiewicz et al.
2018
Kurtyka, R.; Burdach, Z.; Siemieniuk, A.; Karcz, W.
In: Ecotoxicology and Environmental Safety, vol. 161, pp. 8-16, 2018, ISSN: 01476513, (8).
@article{2-s2.0-85047635603,
title = {Single and combined effects of Cd and Pb on the growth, medium pH, membrane potential and metal contents in maize (Zea mays L.) coleoptile segments},
author = { R. Kurtyka and Z. Burdach and A. Siemieniuk and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047635603&doi=10.1016%2fj.ecoenv.2018.05.046&partnerID=40&md5=18ad1f9d7d8a7c274468b9a3fbb3820b},
doi = {10.1016/j.ecoenv.2018.05.046},
issn = {01476513},
year = {2018},
date = {2018-01-01},
journal = {Ecotoxicology and Environmental Safety},
volume = {161},
pages = {8-16},
publisher = {Academic Press},
abstract = {The mechanisms of the toxic effects of Cd and Pb on plant cell growth are still poorly understood. In particular, little is known about their interactive effects, which usually occur in the environment. Moreover, the data that do exist in the literature are controversial. This study describes experiments that were performed with maize (Zea mays) coleoptile segments, which is a classical model system for studies of plant cell elongation growth. Cadmium and lead, which were added at 0.1 mM, reduced the endogenous and IAA-induced elongation growth of maize coleoptile cells. When both metals were added together or in sequence, their effect on IAA-induced growth was more toxic. The medium pH changes, which were measured simultaneously with growth, indicated that while Pb stopped IAA-induced proton extrusion, Cd only partially diminished it. Although Cd was generally more accumulated than Pb in the maize coleoptile segments, when IAA was added together with Pb, it significantly increased the accumulation of the metal. The short-term electrophysiological experiments showed that the addition of Cd caused the depolarisation of the membrane potential (E m ), whereas Pb caused membrane hyperpolarisation. In the long-term electrophysiological experiments, it was found that the Cd-induced E m changes are complex. In conclusion, these results suggest that the effects of Cd and Pb as well as their combination on the elongation growth of maize coleoptile cells and the accumulation of the metals result, among others, from different ionic mechanisms by which each metal change the membrane potential of the cells. © 2018 Elsevier Inc.},
note = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The mechanisms of the toxic effects of Cd and Pb on plant cell growth are still poorly understood. In particular, little is known about their interactive effects, which usually occur in the environment. Moreover, the data that do exist in the literature are controversial. This study describes experiments that were performed with maize (Zea mays) coleoptile segments, which is a classical model system for studies of plant cell elongation growth. Cadmium and lead, which were added at 0.1 mM, reduced the endogenous and IAA-induced elongation growth of maize coleoptile cells. When both metals were added together or in sequence, their effect on IAA-induced growth was more toxic. The medium pH changes, which were measured simultaneously with growth, indicated that while Pb stopped IAA-induced proton extrusion, Cd only partially diminished it. Although Cd was generally more accumulated than Pb in the maize coleoptile segments, when IAA was added together with Pb, it significantly increased the accumulation of the metal. The short-term electrophysiological experiments showed that the addition of Cd caused the depolarisation of the membrane potential (E m ), whereas Pb caused membrane hyperpolarisation. In the long-term electrophysiological experiments, it was found that the Cd-induced E m changes are complex. In conclusion, these results suggest that the effects of Cd and Pb as well as their combination on the elongation growth of maize coleoptile cells and the accumulation of the metals result, among others, from different ionic mechanisms by which each metal change the membrane potential of the cells. © 2018 Elsevier Inc.
Burdach, Z.; Siemieniuk, A.; Trela, Z.; Kurtyka, R.; Karcz, W.
Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles Journal Article
In: BMC Plant Biology, vol. 18, no. 1, 2018, ISSN: 14712229, (8).
@article{2-s2.0-85048040874,
title = {Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles},
author = { Z. Burdach and A. Siemieniuk and Z. Trela and R. Kurtyka and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048040874&doi=10.1186%2fs12870-018-1321-6&partnerID=40&md5=8db44cbfb0dd470943970e766f250d99},
doi = {10.1186/s12870-018-1321-6},
issn = {14712229},
year = {2018},
date = {2018-01-01},
journal = {BMC Plant Biology},
volume = {18},
number = {1},
publisher = {BioMed Central Ltd.},
abstract = {Background: Auxin (IAA) is a central player in plant cell growth. In contrast to the well-established function of the plasma membrane in plant cell expansion, little is known about the role of the vacuolar membrane (tonoplast) in this process. Results: It was found that under symmetrical 100 mM K+ and 100 μM cytoplasmic Ca2+ the macroscopic currents showed a typical slow activation and a strong outward rectification of the steady-state currents. The addition of IAA at a final concentration of 1 μM to the bath medium stimulated the SV currents, whereas at 0.1 and 10 μM slight inhibition of SV currents was observed. The time constant, decreased in the presence of this hormone. When single channels were analyzed, an increase in their activity was recorded with IAA compared to the control. The single-channel recordings that were obtained in the presence of IAA showed that auxin increased the amplitude of the single-channel currents. Interestingly, the addition of IAA to the bath medium with the same composition as the one that was used in the patch-clamp experiments showed that auxin decreased the volume of the vacuoles. Conclusions: It is suggested that the SV channels and the volume of red beet taproot vacuoles are modulated by auxin (IAA). © 2018 The Author(s).},
note = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Background: Auxin (IAA) is a central player in plant cell growth. In contrast to the well-established function of the plasma membrane in plant cell expansion, little is known about the role of the vacuolar membrane (tonoplast) in this process. Results: It was found that under symmetrical 100 mM K+ and 100 μM cytoplasmic Ca2+ the macroscopic currents showed a typical slow activation and a strong outward rectification of the steady-state currents. The addition of IAA at a final concentration of 1 μM to the bath medium stimulated the SV currents, whereas at 0.1 and 10 μM slight inhibition of SV currents was observed. The time constant, decreased in the presence of this hormone. When single channels were analyzed, an increase in their activity was recorded with IAA compared to the control. The single-channel recordings that were obtained in the presence of IAA showed that auxin increased the amplitude of the single-channel currents. Interestingly, the addition of IAA to the bath medium with the same composition as the one that was used in the patch-clamp experiments showed that auxin decreased the volume of the vacuoles. Conclusions: It is suggested that the SV channels and the volume of red beet taproot vacuoles are modulated by auxin (IAA). © 2018 The Author(s).
2015
Trela, Z.; Burdach, Z.; Siemieniuk, A.; Przestalski, S.; Karcz, W.
Effect of trimethyltin chloride on slow vacuolar (SV) channels in vacuoles from red beet (Beta vulgaris L.) taproots Journal Article
In: PLoS ONE, vol. 10, no. 8, 2015, ISSN: 19326203, (3).
@article{2-s2.0-84943329529,
title = {Effect of trimethyltin chloride on slow vacuolar (SV) channels in vacuoles from red beet (Beta vulgaris L.) taproots},
author = { Z. Trela and Z. Burdach and A. Siemieniuk and S. Przestalski and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943329529&doi=10.1371%2fjournal.pone.0136346&partnerID=40&md5=de049663a59a03f2b71dada43afadf3c},
doi = {10.1371/journal.pone.0136346},
issn = {19326203},
year = {2015},
date = {2015-01-01},
journal = {PLoS ONE},
volume = {10},
number = {8},
publisher = {Public Library of Science},
abstract = {In the present study, patch-clamp techniques have been used to investigate the effect of trimethyltin chloride (Met3SnCl) on the slow vacuolar (SV) channels in vacuoles from red beet (Beta vulgaris L.) taproots. Activity of SV channels has been measured in whole-vacuole and cytosolic side-out patch configurations. It was found that addition of trimethyltin chloride to the bath solution suppressed, in a concentration-dependent manner, SV currents in red beet vacuoles. The time constant, τ, increased significantly in the presence of the organotin. When single channel activity was analyzed, only little channel activity could be recorded at 100 μM Met3SnCl. Trimethyltin chloride added to the bath medium significantly decreased (by ca. threefold at 100 μM Met3SnCl and at 100 mV voltage; as compared to the control medium) the open probability of single channels. Single channel recordings obtained in the presence and absence of trimethyltin chloride showed that the organotin only slightly (by <10%) decreased the unitary conductance of single channels. It was also found that Met3SnCl significantly diminished the number of SV channel openings, whereas it did not change the opening times of the channels. Taking into account the above and the fact that under the here applied experimental conditions (pH = 7.5) Met3SnCl is a non-dissociated (more lipophilic) compound, we suggest that the suppression of SV currents observed in the presence of the organotin results probably from its hydrophobic properties allowing this compound to translocate near the selectivity filter of the channel. © 2015 Trela et al.},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In the present study, patch-clamp techniques have been used to investigate the effect of trimethyltin chloride (Met3SnCl) on the slow vacuolar (SV) channels in vacuoles from red beet (Beta vulgaris L.) taproots. Activity of SV channels has been measured in whole-vacuole and cytosolic side-out patch configurations. It was found that addition of trimethyltin chloride to the bath solution suppressed, in a concentration-dependent manner, SV currents in red beet vacuoles. The time constant, τ, increased significantly in the presence of the organotin. When single channel activity was analyzed, only little channel activity could be recorded at 100 μM Met3SnCl. Trimethyltin chloride added to the bath medium significantly decreased (by ca. threefold at 100 μM Met3SnCl and at 100 mV voltage; as compared to the control medium) the open probability of single channels. Single channel recordings obtained in the presence and absence of trimethyltin chloride showed that the organotin only slightly (by <10%) decreased the unitary conductance of single channels. It was also found that Met3SnCl significantly diminished the number of SV channel openings, whereas it did not change the opening times of the channels. Taking into account the above and the fact that under the here applied experimental conditions (pH = 7.5) Met3SnCl is a non-dissociated (more lipophilic) compound, we suggest that the suppression of SV currents observed in the presence of the organotin results probably from its hydrophobic properties allowing this compound to translocate near the selectivity filter of the channel. © 2015 Trela et al.
2014
Burdach, Z.; Kurtyka, R.; Siemieniuk, A.; Karcz, W.
Role of chloride ions in the promotion of auxin-induced growth of maize coleoptile segments Journal Article
In: Annals of Botany, vol. 114, no. 5, pp. 1023-1034, 2014, ISSN: 03057364, (18).
@article{2-s2.0-84922881434,
title = {Role of chloride ions in the promotion of auxin-induced growth of maize coleoptile segments},
author = { Z. Burdach and R. Kurtyka and A. Siemieniuk and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84922881434&doi=10.1093%2faob%2fmcu170&partnerID=40&md5=7ef4c10f60d347b453e47c8f373ba816},
doi = {10.1093/aob/mcu170},
issn = {03057364},
year = {2014},
date = {2014-01-01},
journal = {Annals of Botany},
volume = {114},
number = {5},
pages = {1023-1034},
publisher = {Oxford University Press},
abstract = {Background and Aims: The mechanism of auxin action on ion transport in growing cells has not been determined in detail. In particular, little is known about the role of chloride in the auxin-induced growth of coleoptile cells. Moreover, the data that do exist in the literature are controversial. This study describes experiments that were carried out with maize (Zea mays) coleoptile segments, this being a classical model system for studies of plant cell elongation growth. • Methods: Growth kinetics or growth and pH changes were recorded in maize coleoptiles using two independent measuring systems. The growth rate of the segments was measured simultaneously with medium pH changes. Membrane potential changes in parenchymal cells of the segments were also determined for chosen variants. The question of whether anion transport is involved in auxin-induced growth of maize coleoptile segmentswas primarily studied using anion channel blockers [anthracene-9-carboxylic acid (A-9-C) and 4;4' -diisothiocyanatostilbene-2;2'-disulphonic acid (DIDS)]. In addition, experiments in which KCl was replaced by KNO3 were also performed. • Key Results: Both anion channel blockers, added at 0.1 mM, diminished indole-3-acetic acid (IAA)-induced elongation growth by ∼ 30%. Medium pH changes measured simultaneously with growth indicated that while DIDS stopped IAA-induced proton extrusion, A-9-C diminished it by only 50%. Addition of A-9-C to medium containing 1 mM KCl did not affect the characteristic kinetics of IAA-induced membrane potential changes, while in the presence of 10 mM KCl the channel blocker stopped IAA-induced membrane hyperpolarization. Replacement of KCl with KNO3 significantly decreased IAA-induced growth and inhibited proton extrusion. In contrast to the KCl concentration, the concentration of KNO3 did not affect the growth-stimulatory effect of IAA. For comparison, the effects of the cation channel blocker tetraethylammonium chloride (TEA-Cl) on IAA-induced growth and proton extrusion were also determined. TEA-Cl, added 1 h before IAA, caused reduction of growth by 49.9% and inhibition of proton extrusion. • Conclusions: These results suggest that Cl - plays a role in the IAA-induced growth of maize coleoptile segments. A possible mechanism for Cl- uptake during IAA-induced growth is proposed in which uptake of K+ and Cl- ions in concert with IAA-induced plasma membrane H+ -ATPase activity changes the membrane potential to a value needed for turgor adjustment during the growth of maize coleoptile cells. © The Author 2014.},
note = {18},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Background and Aims: The mechanism of auxin action on ion transport in growing cells has not been determined in detail. In particular, little is known about the role of chloride in the auxin-induced growth of coleoptile cells. Moreover, the data that do exist in the literature are controversial. This study describes experiments that were carried out with maize (Zea mays) coleoptile segments, this being a classical model system for studies of plant cell elongation growth. • Methods: Growth kinetics or growth and pH changes were recorded in maize coleoptiles using two independent measuring systems. The growth rate of the segments was measured simultaneously with medium pH changes. Membrane potential changes in parenchymal cells of the segments were also determined for chosen variants. The question of whether anion transport is involved in auxin-induced growth of maize coleoptile segmentswas primarily studied using anion channel blockers [anthracene-9-carboxylic acid (A-9-C) and 4;4' -diisothiocyanatostilbene-2;2'-disulphonic acid (DIDS)]. In addition, experiments in which KCl was replaced by KNO3 were also performed. • Key Results: Both anion channel blockers, added at 0.1 mM, diminished indole-3-acetic acid (IAA)-induced elongation growth by ∼ 30%. Medium pH changes measured simultaneously with growth indicated that while DIDS stopped IAA-induced proton extrusion, A-9-C diminished it by only 50%. Addition of A-9-C to medium containing 1 mM KCl did not affect the characteristic kinetics of IAA-induced membrane potential changes, while in the presence of 10 mM KCl the channel blocker stopped IAA-induced membrane hyperpolarization. Replacement of KCl with KNO3 significantly decreased IAA-induced growth and inhibited proton extrusion. In contrast to the KCl concentration, the concentration of KNO3 did not affect the growth-stimulatory effect of IAA. For comparison, the effects of the cation channel blocker tetraethylammonium chloride (TEA-Cl) on IAA-induced growth and proton extrusion were also determined. TEA-Cl, added 1 h before IAA, caused reduction of growth by 49.9% and inhibition of proton extrusion. • Conclusions: These results suggest that Cl - plays a role in the IAA-induced growth of maize coleoptile segments. A possible mechanism for Cl- uptake during IAA-induced growth is proposed in which uptake of K+ and Cl- ions in concert with IAA-induced plasma membrane H+ -ATPase activity changes the membrane potential to a value needed for turgor adjustment during the growth of maize coleoptile cells. © The Author 2014.
2012
Trela, Z.; Burdach, Z.; Przestalski, S.; Karcz, W.
Effect of trimethyllead chloride on slowly activating (SV) channels in red beet (Beta vulgaris L.) taproots Journal Article
In: Comptes Rendus - Biologies, vol. 335, no. 12, pp. 722-730, 2012, ISSN: 16310691, (4).
@article{2-s2.0-84872154905,
title = {Effect of trimethyllead chloride on slowly activating (SV) channels in red beet (Beta vulgaris L.) taproots},
author = { Z. Trela and Z. Burdach and S. Przestalski and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872154905&doi=10.1016%2fj.crvi.2012.11.004&partnerID=40&md5=123a4c36295b86d469fe5585a393af80},
doi = {10.1016/j.crvi.2012.11.004},
issn = {16310691},
year = {2012},
date = {2012-01-01},
journal = {Comptes Rendus - Biologies},
volume = {335},
number = {12},
pages = {722-730},
abstract = {The patch-clamp technique was used to examine the effect of trimethyllead chloride (Met3PbCl) on SV channel activity in red beet (Beta vulgaris L.) taproot vacuoles. It was found that in the control bath the macroscopic currents showed the typical slow activation and a strong outward rectification of the steady-state currents. An addition of Met3PbCl to the bath solution blocked, in a concentration-dependent manner, SV currents in red beet vacuoles. The time constant τ increased several times in the presence of 100 μM trimethyllead chloride at all voltages tested. When single channel properties were analyzed, only little channel activity could be recorded in the presence of 100 μM Met3PbCl. Trimethyllead chloride decreased significantly (by about one order of magnitude) the open probability of single channels. The recordings of single channel activity obtained in the presence and absence of Met3PbCl showed that organolead only slightly (by ca. 10%) decreased the unitary conductance of single channels. It was also found that Met3PbCl diminished significantly the number of SV channel openings, whereas it did not change the opening times of the channels. Taken together, these results suggest that Met3PbCl binding site is located outside the channel selectivity filter. © 2012 Académie des sciences.},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The patch-clamp technique was used to examine the effect of trimethyllead chloride (Met3PbCl) on SV channel activity in red beet (Beta vulgaris L.) taproot vacuoles. It was found that in the control bath the macroscopic currents showed the typical slow activation and a strong outward rectification of the steady-state currents. An addition of Met3PbCl to the bath solution blocked, in a concentration-dependent manner, SV currents in red beet vacuoles. The time constant τ increased several times in the presence of 100 μM trimethyllead chloride at all voltages tested. When single channel properties were analyzed, only little channel activity could be recorded in the presence of 100 μM Met3PbCl. Trimethyllead chloride decreased significantly (by about one order of magnitude) the open probability of single channels. The recordings of single channel activity obtained in the presence and absence of Met3PbCl showed that organolead only slightly (by ca. 10%) decreased the unitary conductance of single channels. It was also found that Met3PbCl diminished significantly the number of SV channel openings, whereas it did not change the opening times of the channels. Taken together, these results suggest that Met3PbCl binding site is located outside the channel selectivity filter. © 2012 Académie des sciences.
Kurtyka, R.; Małkowski, E.; Burdach, Z.; Kita, A.; Karcz, W.
Interactive effects of temperature and heavy metals (Cd, Pb) on the elongation growth in maize coleoptiles Journal Article
In: Comptes Rendus - Biologies, vol. 335, no. 4, pp. 292-299, 2012, ISSN: 16310691, (7).
@article{2-s2.0-84861099047,
title = {Interactive effects of temperature and heavy metals (Cd, Pb) on the elongation growth in maize coleoptiles},
author = { R. Kurtyka and E. Małkowski and Z. Burdach and A. Kita and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861099047&doi=10.1016%2fj.crvi.2012.03.012&partnerID=40&md5=4346ff3f622d042a44a2211c75624cb4},
doi = {10.1016/j.crvi.2012.03.012},
issn = {16310691},
year = {2012},
date = {2012-01-01},
journal = {Comptes Rendus - Biologies},
volume = {335},
number = {4},
pages = {292-299},
abstract = {The effect of Cd and Pb on endogenous and IAA-induced elongation growth and medium pH of maize coleoptile segments incubated at 20, 25 and 30 °C was studied. It was found that the elongation of coleoptile segments and proton extrusion increased with the temperature and reached its maximum at 30 °C. For Cd, the maximal inhibition of endogenous and IAA-induced growth as well as medium acidification of coleoptile segments was observed at 25 °C. Meanwhile, Pb, irrespective of the temperature, diminished the growth of the segments by ca. 20%, increasing the acidification of the incubation medium. It was also found that in contrast to Cd, Pb accumulation in maize coleoptile segments did not correlate with temperature. The results suggest that the toxic effect of Cd on elongation growth of coleoptile segments is connected with the decrease of the PM H +-ATPase activity and probably with Cd-induced high acivity of IAA oxidase, whereas the effect of Pb did not depend on activity of any of the enzymes. © 2012 Académie des sciences.},
note = {7},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effect of Cd and Pb on endogenous and IAA-induced elongation growth and medium pH of maize coleoptile segments incubated at 20, 25 and 30 °C was studied. It was found that the elongation of coleoptile segments and proton extrusion increased with the temperature and reached its maximum at 30 °C. For Cd, the maximal inhibition of endogenous and IAA-induced growth as well as medium acidification of coleoptile segments was observed at 25 °C. Meanwhile, Pb, irrespective of the temperature, diminished the growth of the segments by ca. 20%, increasing the acidification of the incubation medium. It was also found that in contrast to Cd, Pb accumulation in maize coleoptile segments did not correlate with temperature. The results suggest that the toxic effect of Cd on elongation growth of coleoptile segments is connected with the decrease of the PM H +-ATPase activity and probably with Cd-induced high acivity of IAA oxidase, whereas the effect of Pb did not depend on activity of any of the enzymes. © 2012 Académie des sciences.
2011
Kurtyka, R.; Burdach, Z.; Karcz, W.
Temperature dependence of growth in maize seedlings and excised coleoptile segments Journal Article
In: Polish Journal of Environmental Studies, vol. 20, no. 5, pp. 1221-1225, 2011, ISSN: 12301485, (4).
@article{2-s2.0-80054026320,
title = {Temperature dependence of growth in maize seedlings and excised coleoptile segments},
author = { R. Kurtyka and Z. Burdach and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80054026320&partnerID=40&md5=81d96c75404438c30eec0a973b6ba843},
issn = {12301485},
year = {2011},
date = {2011-01-01},
journal = {Polish Journal of Environmental Studies},
volume = {20},
number = {5},
pages = {1221-1225},
abstract = {The effect of temperature in the range 22-32°C and 25-40°C on elongation growth of maize seedlings and coleoptiles excised from them was studied, respectively. It was found that elongation of maize seedling's organs (root; mesocotyl and coleoptile) increased with increasing temperature in the range 22-32°C. However, when coleoptile segments excised from seedlings grown at highest temperature (32°C) were incubated at various temperatures (25; 30; 35; and 40°C) and in the presence of IAA their elongation growth was always lower compared to segments excised from seedlings grown at 22 and 27°C. Simultaneous measurements of elongation growth and external medium pH indicated that proton extrusion increased with increasing medium temperature from 25 to 35°C. The results presented here show that the temperatures at which the maize seedlings were cultivated are crucial for temperature dependence of a coleoptile segment's elongation growth.},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effect of temperature in the range 22-32°C and 25-40°C on elongation growth of maize seedlings and coleoptiles excised from them was studied, respectively. It was found that elongation of maize seedling's organs (root; mesocotyl and coleoptile) increased with increasing temperature in the range 22-32°C. However, when coleoptile segments excised from seedlings grown at highest temperature (32°C) were incubated at various temperatures (25; 30; 35; and 40°C) and in the presence of IAA their elongation growth was always lower compared to segments excised from seedlings grown at 22 and 27°C. Simultaneous measurements of elongation growth and external medium pH indicated that proton extrusion increased with increasing medium temperature from 25 to 35°C. The results presented here show that the temperatures at which the maize seedlings were cultivated are crucial for temperature dependence of a coleoptile segment's elongation growth.
Kurtyka, R.; Burdach, Z.; Karcz, W.
Effect of cadmium and lead on the membrane potential and photoelectric reaction of Nitellopsis obtusa cells Journal Article
In: General Physiology and Biophysics, vol. 30, no. 1, pp. 52-58, 2011, ISSN: 02315882, (12).
@article{2-s2.0-79953688700,
title = {Effect of cadmium and lead on the membrane potential and photoelectric reaction of Nitellopsis obtusa cells},
author = { R. Kurtyka and Z. Burdach and W. Karcz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79953688700&doi=10.4149%2fgpb_2011_01_52&partnerID=40&md5=a317763ee317c849a0eafef55067489b},
doi = {10.4149/gpb_2011_01_52},
issn = {02315882},
year = {2011},
date = {2011-01-01},
journal = {General Physiology and Biophysics},
volume = {30},
number = {1},
pages = {52-58},
abstract = {The effects of Cd and Pb on membrane potential (Em) and photoelectric reaction of Nitellopsis obtusa cells were investigated. It was found that Cd and Pb at 1.0 mM caused a depolarization of the Em, whereas both metals at lower concentrations changed the Em in a different way. Pb at 0.1 mM and 0.01 mM hyperpolarized the Em, whereas Cd at the same concentrations depolarized and did not change the E m, respectively. In the presence of 0.01 mM Pb, the light-induced hyperpolarization of the Em was by 18% higher as compared to the control, whereas at 1.0 mM Pb it was by 40% lower. Pb at 0.1 mM and Cd at 0.01 mM or 5 x 0.01 mM did not change the light-induced membrane hyperpolarization. However, in the presence of Cd at 0.1 mM and 1.0 mM this hyperpolarization was 2-fold lower or was completely abolished, respectively. These results suggest that at high Cd and Pb concentrations both depolarization of the Em and decrease of light-induced membrane hyperpolarization in Nitellopsis obtusa cells are probably due to inhibition of the plasma membrane H+-ATPase activity, whereas both metals at lower concentrations differ in mechanism of membrane potential changes.},
note = {12},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effects of Cd and Pb on membrane potential (Em) and photoelectric reaction of Nitellopsis obtusa cells were investigated. It was found that Cd and Pb at 1.0 mM caused a depolarization of the Em, whereas both metals at lower concentrations changed the Em in a different way. Pb at 0.1 mM and 0.01 mM hyperpolarized the Em, whereas Cd at the same concentrations depolarized and did not change the E m, respectively. In the presence of 0.01 mM Pb, the light-induced hyperpolarization of the Em was by 18% higher as compared to the control, whereas at 1.0 mM Pb it was by 40% lower. Pb at 0.1 mM and Cd at 0.01 mM or 5 x 0.01 mM did not change the light-induced membrane hyperpolarization. However, in the presence of Cd at 0.1 mM and 1.0 mM this hyperpolarization was 2-fold lower or was completely abolished, respectively. These results suggest that at high Cd and Pb concentrations both depolarization of the Em and decrease of light-induced membrane hyperpolarization in Nitellopsis obtusa cells are probably due to inhibition of the plasma membrane H+-ATPase activity, whereas both metals at lower concentrations differ in mechanism of membrane potential changes.
2007
Karcz, W.; Burdach, Z.
Effect of temperature on growth, proton extrusion and membrane potential in maize (Zea mays L.) coleoptile segments Journal Article
In: Plant Growth Regulation, vol. 52, no. 2, pp. 141-150, 2007, ISSN: 01676903, (15).
@article{2-s2.0-34250668034,
title = {Effect of temperature on growth, proton extrusion and membrane potential in maize (Zea mays L.) coleoptile segments},
author = { W. Karcz and Z. Burdach},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250668034&doi=10.1007%2fs10725-007-9184-0&partnerID=40&md5=9119ad97aaab51c5ae2b8cf60e2dd54f},
doi = {10.1007/s10725-007-9184-0},
issn = {01676903},
year = {2007},
date = {2007-01-01},
journal = {Plant Growth Regulation},
volume = {52},
number = {2},
pages = {141-150},
abstract = {The effects of temperature (5-45°C) on endogenous growth, growth in the presence of either indoleacetic acid (IAA) or fusicoccin (FC), and proton extrusion in maize coleoptile segments were studied. In addition, membrane potential changes at some temperatures were also determined. It was found that in this model system endogenous growth exhibits a clear maximum at 30°C, whereas growth in the presence of IAA and FC shows the maximum value in the range 30-35°C and 35-40°C, respectively. Simultaneous measurements of growth and external medium pH indicated that FC at stressful temperatures was not only much more active in the stimulation of growth, but was also more effective in acidifying the external medium than IAA. Also the addition of either IAA or FC to the bathing medium at 30 and 40°C did not change the kinetic characteristic of membrane potential changes observed for both substances at 25°C. However, the increased temperature significantly decreased IAA and FC-induced membrane hyperpolarization. IAA in the incubation medium, at 10°C, brought about additional membrane depolarization (apart from the one induced by low temperature). In contrast to IAA, FC at 10°C caused gradual repolarization of membrane potential, which correlated with both FC-induced growth and FC-induced proton extrusion. A plausible interpretation for temperature-induced changes in growth of maize coleoptile segments is that, at least in part, these changes were mediated via a PM H+-ATPase activity. © 2007 Springer Science+Business Media B.V.},
note = {15},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effects of temperature (5-45°C) on endogenous growth, growth in the presence of either indoleacetic acid (IAA) or fusicoccin (FC), and proton extrusion in maize coleoptile segments were studied. In addition, membrane potential changes at some temperatures were also determined. It was found that in this model system endogenous growth exhibits a clear maximum at 30°C, whereas growth in the presence of IAA and FC shows the maximum value in the range 30-35°C and 35-40°C, respectively. Simultaneous measurements of growth and external medium pH indicated that FC at stressful temperatures was not only much more active in the stimulation of growth, but was also more effective in acidifying the external medium than IAA. Also the addition of either IAA or FC to the bathing medium at 30 and 40°C did not change the kinetic characteristic of membrane potential changes observed for both substances at 25°C. However, the increased temperature significantly decreased IAA and FC-induced membrane hyperpolarization. IAA in the incubation medium, at 10°C, brought about additional membrane depolarization (apart from the one induced by low temperature). In contrast to IAA, FC at 10°C caused gradual repolarization of membrane potential, which correlated with both FC-induced growth and FC-induced proton extrusion. A plausible interpretation for temperature-induced changes in growth of maize coleoptile segments is that, at least in part, these changes were mediated via a PM H+-ATPase activity. © 2007 Springer Science+Business Media B.V.
2002
Karcz, W.; Burdach, Z.
A comparison of the effects of IAA and 4-CI-IAA on growth, proton secretion and membrane potential in maize coleoptile segments Journal Article
In: Journal of Experimental Botany, vol. 53, no. 371, pp. 1089-1098, 2002, ISSN: 00220957, (42).
@article{2-s2.0-0036011316,
title = {A comparison of the effects of IAA and 4-CI-IAA on growth, proton secretion and membrane potential in maize coleoptile segments},
author = { W. Karcz and Z. Burdach},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036011316&partnerID=40&md5=5119f1679989f6016b164e99af749347},
issn = {00220957},
year = {2002},
date = {2002-01-01},
journal = {Journal of Experimental Botany},
volume = {53},
number = {371},
pages = {1089-1098},
abstract = {The physiological activity of exogenous 4-CI-IAA, as compared to IAA, was examined in maize coleoptile segments. It was found that in this model system 4-CI-IAA is much more active in the stimulation of elongation than IAA. Simultaneous measurements of growth and external pH indicated that administration of either IAA or 4-CI-IAA resulted in medium acidification. The kinetics of the pH changes, however, were faster after the addition of 4-CI-IAA. In contrast to IAA, the coleoptile segments treated with chlorinated auxin were not able to increase medium pH after its initial drop. The re-addition of IAA after 5 h further enhanced growth over the next 2 h by 31%. By contrast, the re-addition of 4-CI-IAA at the same time protocol as IAA did not cause an additional effect. The administration of 10 μM IAA induced in maize coleoptile cells a transient depolarization followed by a slow hyperpolarization of their membrane potential. In contrast to IAA, 4-CI-IAA at 1 μM caused an immediate hyperpolarization of the membrane potential which, on average, was 2-fold greater than for IAA. The results reported here provide further evidence that 4-CI-IAA is much more active, as compared to IAA, in stimulating the growth of maize coleoptile segments. Although it has not been directly demonstrated here, a plausible interpretation for the high 4-CI-IAA activity is that, at least in part, it might be caused via a reduced metabolism of 4-CI-IAA. Furthermore, for the first time, the data show that membrane potential responds to 4-CI-IAA in a qualitatively different fashion than to IAA. These findings may, in turn, suggest a specific signal transduction pathway to 4-CI-IAA in maize coleoptile cells.},
note = {42},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The physiological activity of exogenous 4-CI-IAA, as compared to IAA, was examined in maize coleoptile segments. It was found that in this model system 4-CI-IAA is much more active in the stimulation of elongation than IAA. Simultaneous measurements of growth and external pH indicated that administration of either IAA or 4-CI-IAA resulted in medium acidification. The kinetics of the pH changes, however, were faster after the addition of 4-CI-IAA. In contrast to IAA, the coleoptile segments treated with chlorinated auxin were not able to increase medium pH after its initial drop. The re-addition of IAA after 5 h further enhanced growth over the next 2 h by 31%. By contrast, the re-addition of 4-CI-IAA at the same time protocol as IAA did not cause an additional effect. The administration of 10 μM IAA induced in maize coleoptile cells a transient depolarization followed by a slow hyperpolarization of their membrane potential. In contrast to IAA, 4-CI-IAA at 1 μM caused an immediate hyperpolarization of the membrane potential which, on average, was 2-fold greater than for IAA. The results reported here provide further evidence that 4-CI-IAA is much more active, as compared to IAA, in stimulating the growth of maize coleoptile segments. Although it has not been directly demonstrated here, a plausible interpretation for the high 4-CI-IAA activity is that, at least in part, it might be caused via a reduced metabolism of 4-CI-IAA. Furthermore, for the first time, the data show that membrane potential responds to 4-CI-IAA in a qualitatively different fashion than to IAA. These findings may, in turn, suggest a specific signal transduction pathway to 4-CI-IAA in maize coleoptile cells.
1995
Karcz, W.; Burdach, Z.
The effects of electric field on the growth of intact seedlings and coleoptile segments of Zea mays L. Journal Article
In: Biologia Plantarum, vol. 37, no. 3, pp. 391-397, 1995, ISSN: 00063134, (8).
@article{2-s2.0-0028870295,
title = {The effects of electric field on the growth of intact seedlings and coleoptile segments of Zea mays L.},
author = { W. Karcz and Z. Burdach},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028870295&doi=10.1007%2fBF02913986&partnerID=40&md5=d349f53f8847af308b72fe53e8f3f163},
doi = {10.1007/BF02913986},
issn = {00063134},
year = {1995},
date = {1995-01-01},
journal = {Biologia Plantarum},
volume = {37},
number = {3},
pages = {391-397},
publisher = {Springer Netherlands},
abstract = {The experiments were carried out with 96-h-old intact maize seedlings and 10 mm long coleoptile segments cut 4 mm below the tip. The electric fields were applied longitudinally along the seedlings. The electric field (15 V) caused inhibition of the elongation growth of intact seedlings which was dependent on both the polarity and the duration of the applied voltage. The growth inhibition was greater when the tip of the shoot was positive relative to the roots. The electric field also caused inhibition of indole-3-acetic acid (IAA) and fusicoccin (FC) induced growth of maize coleoptile segments excised from electrically treated seedlings. IAA-induced growth of coleoptile segments was greater when the tip of the shoot was negative to the roots (not in the case of FC-treated segments and intact seedlings). It was suggested that apart from the changes induced by electric field in transport system of auxin the electric field affected also the activity of plasmalemma proton pump. © 1995 Institute of Experimental Botany, ASCR.},
note = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The experiments were carried out with 96-h-old intact maize seedlings and 10 mm long coleoptile segments cut 4 mm below the tip. The electric fields were applied longitudinally along the seedlings. The electric field (15 V) caused inhibition of the elongation growth of intact seedlings which was dependent on both the polarity and the duration of the applied voltage. The growth inhibition was greater when the tip of the shoot was positive relative to the roots. The electric field also caused inhibition of indole-3-acetic acid (IAA) and fusicoccin (FC) induced growth of maize coleoptile segments excised from electrically treated seedlings. IAA-induced growth of coleoptile segments was greater when the tip of the shoot was negative to the roots (not in the case of FC-treated segments and intact seedlings). It was suggested that apart from the changes induced by electric field in transport system of auxin the electric field affected also the activity of plasmalemma proton pump. © 1995 Institute of Experimental Botany, ASCR.
Karcz, W.; Stolarek, J.; Lekacz, H.; Kurtyka, R.; Burdach, Z.
Comparative investigation of auxin and fusicoccin-induced growth and H+-extrusion in coleoptile segments of Zea mays L. Journal Article
In: Acta Physiologiae Plantarum, vol. 17, no. 1, pp. 3-8, 1995, ISSN: 01375881, (21).
@article{2-s2.0-0029167655,
title = {Comparative investigation of auxin and fusicoccin-induced growth and H+-extrusion in coleoptile segments of Zea mays L.},
author = { W. Karcz and J. Stolarek and H. Lekacz and R. Kurtyka and Z. Burdach},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029167655&partnerID=40&md5=7755bf1251e0f139cd47c60d5d3428b4},
issn = {01375881},
year = {1995},
date = {1995-01-01},
journal = {Acta Physiologiae Plantarum},
volume = {17},
number = {1},
pages = {3-8},
abstract = {[No abstract available]},
note = {21},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
[No abstract available]