• dr Joanna Żur-Pińska
Stanowisko: Adiunkt
Jednostka: Wydział Nauk Przyrodniczych
Adres: 40-032 Katowice, ul. Jagiellońska 28
Piętro: I
Numer pokoju: C-151
Telefon: (32) 2009 462
E-mail: joanna.zur@us.edu.pl
Spis publikacji: Spis wg CINiBA
Spis publikacji: Spis wg OPUS
Scopus Author ID: 57193328815
Publikacje z bazy Scopus
2023
Pacholak, A.; Żur, J.; Piński, A.; Nguyen, Q. A.; Ligaj, M.; Łuczak, M.; Nghiem, L. D.; Kaczorek, E.
Potential negative effect of long-term exposure to nitrofurans on bacteria isolated from wastewater Journal Article
In: Science of the Total Environment, vol. 872, 2023, ISSN: 00489697, (2).
@article{2-s2.0-85148334670,
title = {Potential negative effect of long-term exposure to nitrofurans on bacteria isolated from wastewater},
author = { A. Pacholak and J. Żur and A. Piński and Q.A. Nguyen and M. Ligaj and M. Łuczak and L.D. Nghiem and E. Kaczorek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85148334670&doi=10.1016%2fj.scitotenv.2023.162199&partnerID=40&md5=4f73dc7c51328440a75445282b4b764e},
doi = {10.1016/j.scitotenv.2023.162199},
issn = {00489697},
year = {2023},
date = {2023-01-01},
journal = {Science of the Total Environment},
volume = {872},
publisher = {Elsevier B.V.},
abstract = {Nitrofurans are broad-spectrum bactericidal agents used in a large quantity for veterinary and human therapy. This study reports the long-term impact of two nitrofuran representatives, nitrofurantoin (NFT) and furaltadone (FTD) on the bacterial strains Sphingobacterium siyangense FTD2, Achromobacter pulmonis NFZ2, and Stenotrophomonas maltophilia FZD2, isolated from a full-scale wastewater treatment plant. Bacterial whole genome sequencing was used for preliminary strains characterization. The metabolomic, electrochemical, and culture methods were applied to understand changes in the bacterial strains after 12-month exposure to nitrofurans. The most significantly altered metabolic pathways were observed in amino acid and sugar metabolism, and aminoacyl-tRNA biosynthesis. Disrupted protein biosynthesis was measured in all strains treated with antibiotics. Prolonged exposure to NFT and FTD also triggered mutagenic effects, affected metabolic activity, and facilitated oxidative stress within the cells. Nitrofuran-induced oxidative stress was evidenced from an elevated activity of catalase and glutathione S-transferases. NFT and FTD elicited similar but not identical responses in all analyzed strains. The results obtained in this study provide new insights into the potential risks of the prolonged presence of antimicrobial compounds in the environment and contribute to a better understanding of the possible impacts of nitrofuran antibiotics on the bacterial cells. © 2023 Elsevier B.V.},
note = {2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Nitrofurans are broad-spectrum bactericidal agents used in a large quantity for veterinary and human therapy. This study reports the long-term impact of two nitrofuran representatives, nitrofurantoin (NFT) and furaltadone (FTD) on the bacterial strains Sphingobacterium siyangense FTD2, Achromobacter pulmonis NFZ2, and Stenotrophomonas maltophilia FZD2, isolated from a full-scale wastewater treatment plant. Bacterial whole genome sequencing was used for preliminary strains characterization. The metabolomic, electrochemical, and culture methods were applied to understand changes in the bacterial strains after 12-month exposure to nitrofurans. The most significantly altered metabolic pathways were observed in amino acid and sugar metabolism, and aminoacyl-tRNA biosynthesis. Disrupted protein biosynthesis was measured in all strains treated with antibiotics. Prolonged exposure to NFT and FTD also triggered mutagenic effects, affected metabolic activity, and facilitated oxidative stress within the cells. Nitrofuran-induced oxidative stress was evidenced from an elevated activity of catalase and glutathione S-transferases. NFT and FTD elicited similar but not identical responses in all analyzed strains. The results obtained in this study provide new insights into the potential risks of the prolonged presence of antimicrobial compounds in the environment and contribute to a better understanding of the possible impacts of nitrofuran antibiotics on the bacterial cells. © 2023 Elsevier B.V.
2022
Chlebek, D.; Tame, T. M.; Piński, A.; Żur, J.; Hupert-Kocurek, K. T.
Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic Strain Serratia quinivorans KP32 to Fungal Phytopathogens Journal Article
In: International Journal of Molecular Sciences, vol. 23, no. 24, 2022, ISSN: 16616596, (1).
@article{2-s2.0-85144688398,
title = {Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic Strain Serratia quinivorans KP32 to Fungal Phytopathogens},
author = { D. Chlebek and T.M. Tame and A. Piński and J. Żur and K.T. Hupert-Kocurek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144688398&doi=10.3390%2fijms232415561&partnerID=40&md5=8d122b0a287be7d39297e5d8632c6b68},
doi = {10.3390/ijms232415561},
issn = {16616596},
year = {2022},
date = {2022-01-01},
journal = {International Journal of Molecular Sciences},
volume = {23},
number = {24},
publisher = {MDPI},
abstract = {Fungal phytopathogens are challenging to control due to their penetration into plant tissues. Therefore, plant-colonizing bacteria could serve as an excellent weapon in fighting fungal infections. In this study, we aim to determine the biocontrol potential of the new endophytic strain Serratia quinivorans KP32, isolated from the roots of Petroselinum crispum L.; identify the related mechanisms; and understand the basis of its antagonistic interaction with taxonomically diverse fungi at the molecular level. The KP32 strain presented biological activity against Rhizoctonia solani, Colletotrichum dematium, Fusarium avenaceum, and Sclerotinia sclerotiorum, and its ability to inhibit the growth of the phytopathogens was found to be mediated by a broad spectrum of biocontrol features, such as the production of a number of lytic enzymes (amylases; chitinases; and proteases), siderophores, volatile organic and inorganic compounds, salicylic acid, and N-acyl-homoserine lactones. The higher expression of chitinase (chiA) and genes involved in the biosynthesis of hydrogen cyanide (hcnC), enterobactin (entB), and acetoin (budA) in bacteria exposed to fungal filtrates confirmed that these factors could act in combination, leading to a synergistic inhibitory effect of the strain against phytopathogens. We also confirm the active movement, self-aggregation, exopolysaccharide production, and biofilm formation abilities of the KP32 strain, which are essential for effective plant colonization. Its biological activity and colonization potential indicate that KP32 holds tremendous potential for use as an active biopesticide and plant growth promoter. © 2022 by the authors.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Fungal phytopathogens are challenging to control due to their penetration into plant tissues. Therefore, plant-colonizing bacteria could serve as an excellent weapon in fighting fungal infections. In this study, we aim to determine the biocontrol potential of the new endophytic strain Serratia quinivorans KP32, isolated from the roots of Petroselinum crispum L.; identify the related mechanisms; and understand the basis of its antagonistic interaction with taxonomically diverse fungi at the molecular level. The KP32 strain presented biological activity against Rhizoctonia solani, Colletotrichum dematium, Fusarium avenaceum, and Sclerotinia sclerotiorum, and its ability to inhibit the growth of the phytopathogens was found to be mediated by a broad spectrum of biocontrol features, such as the production of a number of lytic enzymes (amylases; chitinases; and proteases), siderophores, volatile organic and inorganic compounds, salicylic acid, and N-acyl-homoserine lactones. The higher expression of chitinase (chiA) and genes involved in the biosynthesis of hydrogen cyanide (hcnC), enterobactin (entB), and acetoin (budA) in bacteria exposed to fungal filtrates confirmed that these factors could act in combination, leading to a synergistic inhibitory effect of the strain against phytopathogens. We also confirm the active movement, self-aggregation, exopolysaccharide production, and biofilm formation abilities of the KP32 strain, which are essential for effective plant colonization. Its biological activity and colonization potential indicate that KP32 holds tremendous potential for use as an active biopesticide and plant growth promoter. © 2022 by the authors.
Wojcieszyńska, D.; Klamka, J.; Marchlewicz, A.; Potocka, I. W.; Żur, J.; Guzik, U.
Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation Journal Article
In: Molecules, vol. 27, no. 18, 2022, ISSN: 14203049, (1).
@article{2-s2.0-85138690790,
title = {Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation},
author = { D. Wojcieszyńska and J. Klamka and A. Marchlewicz and I.W. Potocka and J. Żur and U. Guzik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138690790&doi=10.3390%2fmolecules27185795&partnerID=40&md5=2dda4f9763acf86cf0ed785d52ea3576},
doi = {10.3390/molecules27185795},
issn = {14203049},
year = {2022},
date = {2022-01-01},
journal = {Molecules},
volume = {27},
number = {18},
publisher = {MDPI},
abstract = {Immobilization is a commonly used method in response to the need to increase the resistance of microorganisms to the toxic effects of xenobiotics. In this study, a plant sponge from Luffa cylindrica was used as a carrier for the immobilization of the Stenotrophomonas maltophilia KB2 strain since such a carrier meets the criteria for high-quality carriers, i.e., low price and biodegradability. The optimal immobilization conditions were established as a temperature of 30 °C, pH 7.2, incubation time of 72 h, and an optical density of the culture of 1.4. The strain immobilized in such conditions was used for the biodegradation of naproxen, and an average rate of degradation of 3.8 µg/hour was obtained under cometabolic conditions with glucose. The obtained results indicate that a microbiological preparation based on immobilized cells on a luffa sponge can be used in bioremediation processes where it is necessary to remove the introduced carrier. © 2022 by the authors.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Immobilization is a commonly used method in response to the need to increase the resistance of microorganisms to the toxic effects of xenobiotics. In this study, a plant sponge from Luffa cylindrica was used as a carrier for the immobilization of the Stenotrophomonas maltophilia KB2 strain since such a carrier meets the criteria for high-quality carriers, i.e., low price and biodegradability. The optimal immobilization conditions were established as a temperature of 30 °C, pH 7.2, incubation time of 72 h, and an optical density of the culture of 1.4. The strain immobilized in such conditions was used for the biodegradation of naproxen, and an average rate of degradation of 3.8 µg/hour was obtained under cometabolic conditions with glucose. The obtained results indicate that a microbiological preparation based on immobilized cells on a luffa sponge can be used in bioremediation processes where it is necessary to remove the introduced carrier. © 2022 by the authors.
2021
Żur, J.; Marchlewicz, A.; Piński, A.; Guzik, U.; Wojcieszyńska, D.
Degradation of diclofenac by new bacterial strains and its influence on the physiological status of cells Journal Article
In: Journal of Hazardous Materials, vol. 403, 2021, ISSN: 03043894, (9).
@article{2-s2.0-85092155502,
title = {Degradation of diclofenac by new bacterial strains and its influence on the physiological status of cells},
author = { J. Żur and A. Marchlewicz and A. Piński and U. Guzik and D. Wojcieszyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092155502&doi=10.1016%2fj.jhazmat.2020.124000&partnerID=40&md5=ea0a1291c85c273eacde445aa45e1c1c},
doi = {10.1016/j.jhazmat.2020.124000},
issn = {03043894},
year = {2021},
date = {2021-01-01},
journal = {Journal of Hazardous Materials},
volume = {403},
publisher = {Elsevier B.V.},
abstract = {Diclofenac (DCF) is one of the most commonly utilized non-steroidal anti-inflammatory drugs (NSAIDs), which is known to pose an ecotoxicological threat. In this study, from activated sludge and contaminated soil, we isolated four new bacterial strains able to degrade DCF under mono-substrate and co-metabolic conditions with glucose supplementation. We found that the effectiveness of DCF removal is strictly strain-specific and the addition of the primary substrate is not always beneficial. To assess the multidirectional influence of DCF on bacterial cells we evaluated the alterations of increasing concentrations of this drug on membrane structure. A significant increase was observed in the content of 17:0 cyclo fatty acid, which is responsible for reduced fluidity and profound changes in membrane rigidity. The cell injury and oxidative stress were assessed with biomarkers used as endpoints of toxicity, i.e. catalase (CAT), superoxide dismutase (SOD), lipids peroxidation (LPX), and both intra- and extracellular alkaline and acid phosphatase activity. Results indicated that DCF induced oxidative stress, frequently intensified by the addition of glucose. However, the response of the microbial cells to the presence of DCF should not be generalized, since the overall picture of the particular alterations greatly varied for each of the examined strains. © 2020 Elsevier B.V.},
note = {9},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Diclofenac (DCF) is one of the most commonly utilized non-steroidal anti-inflammatory drugs (NSAIDs), which is known to pose an ecotoxicological threat. In this study, from activated sludge and contaminated soil, we isolated four new bacterial strains able to degrade DCF under mono-substrate and co-metabolic conditions with glucose supplementation. We found that the effectiveness of DCF removal is strictly strain-specific and the addition of the primary substrate is not always beneficial. To assess the multidirectional influence of DCF on bacterial cells we evaluated the alterations of increasing concentrations of this drug on membrane structure. A significant increase was observed in the content of 17:0 cyclo fatty acid, which is responsible for reduced fluidity and profound changes in membrane rigidity. The cell injury and oxidative stress were assessed with biomarkers used as endpoints of toxicity, i.e. catalase (CAT), superoxide dismutase (SOD), lipids peroxidation (LPX), and both intra- and extracellular alkaline and acid phosphatase activity. Results indicated that DCF induced oxidative stress, frequently intensified by the addition of glucose. However, the response of the microbial cells to the presence of DCF should not be generalized, since the overall picture of the particular alterations greatly varied for each of the examined strains. © 2020 Elsevier B.V.
2020
Chlebek, D.; Piński, A.; Żur, J.; Michalska, J.; Hupert-Kocurek, K. T.
In: International Journal of Molecular Sciences, vol. 21, no. 22, pp. 1-21, 2020, ISSN: 16616596, (13).
@article{2-s2.0-85096339783,
title = {Genome mining and evaluation of the biocontrol potential of pseudomonas fluorescens brz63, a new endophyte of oilseed rape (Brassica napus l.) against fungal pathogens},
author = { D. Chlebek and A. Piński and J. Żur and J. Michalska and K.T. Hupert-Kocurek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096339783&doi=10.3390%2fijms21228740&partnerID=40&md5=87d4457488d36bc724971e34dcfdacb3},
doi = {10.3390/ijms21228740},
issn = {16616596},
year = {2020},
date = {2020-01-01},
journal = {International Journal of Molecular Sciences},
volume = {21},
number = {22},
pages = {1-21},
publisher = {MDPI AG},
abstract = {Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {13},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Żur, J.; Piński, A.; Wojcieszyńska, D.; Smułek, W.; Guzik, U.
Diclofenac degradation—enzymes, genetic background and cellular alterations triggered in diclofenac-metabolizing strain pseudomonas moorei KB4 Journal Article
In: International Journal of Molecular Sciences, vol. 21, no. 18, pp. 1-22, 2020, ISSN: 16616596, (7).
@article{2-s2.0-85090899825,
title = {Diclofenac degradation—enzymes, genetic background and cellular alterations triggered in diclofenac-metabolizing strain pseudomonas moorei KB4},
author = { J. Żur and A. Piński and D. Wojcieszyńska and W. Smułek and U. Guzik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090899825&doi=10.3390%2fijms21186786&partnerID=40&md5=02cb0c3ff66c56b2a75244c5e93fb01b},
doi = {10.3390/ijms21186786},
issn = {16616596},
year = {2020},
date = {2020-01-01},
journal = {International Journal of Molecular Sciences},
volume = {21},
number = {18},
pages = {1-22},
publisher = {MDPI AG},
abstract = {Diclofenac (DCF) constitutes one of the most significant ecopollutants detected in various environmental matrices. Biological clean-up technologies that rely on xenobiotics-degrading microorganisms are considered as a valuable alternative for chemical oxidation methods. Up to now, the knowledge about DCF multi-level influence on bacterial cells is fragmentary. In this study, we evaluate the degradation potential and impact of DCF on Pseudomonas moorei KB4 strain. In mono-substrate culture KB4 metabolized 0.5 mg L−1 of DCF, but supplementation with glucose (Glc) and sodium acetate (SA) increased degraded doses up to 1 mg L−1 within 12 days. For all established conditions, 4′-OH-DCF and DCF-lactam were identified. Gene expression analysis revealed the up-regulation of selected genes encoding biotransformation enzymes in the presence of DCF, in both mono-substrate and co-metabolic conditions. The multifactorial analysis of KB4 cell exposure to DCF showed a decrease in the zeta-potential with a simultaneous increase in the cell wall hydrophobicity. Magnified membrane permeability was coupled with the significant increase in the branched (19:0 anteiso) and cyclopropane (17:0 cyclo) fatty acid accompanied with reduced amounts of unsaturated ones. DCF injures the cells which is expressed by raised activities of acid and alkaline phosphatases as well as formation of lipids peroxidation products (LPX). The elevated activity of superoxide dismutase (SOD) and catalase (CAT) testified that DCF induced oxidative stress. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {7},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Diclofenac (DCF) constitutes one of the most significant ecopollutants detected in various environmental matrices. Biological clean-up technologies that rely on xenobiotics-degrading microorganisms are considered as a valuable alternative for chemical oxidation methods. Up to now, the knowledge about DCF multi-level influence on bacterial cells is fragmentary. In this study, we evaluate the degradation potential and impact of DCF on Pseudomonas moorei KB4 strain. In mono-substrate culture KB4 metabolized 0.5 mg L−1 of DCF, but supplementation with glucose (Glc) and sodium acetate (SA) increased degraded doses up to 1 mg L−1 within 12 days. For all established conditions, 4′-OH-DCF and DCF-lactam were identified. Gene expression analysis revealed the up-regulation of selected genes encoding biotransformation enzymes in the presence of DCF, in both mono-substrate and co-metabolic conditions. The multifactorial analysis of KB4 cell exposure to DCF showed a decrease in the zeta-potential with a simultaneous increase in the cell wall hydrophobicity. Magnified membrane permeability was coupled with the significant increase in the branched (19:0 anteiso) and cyclopropane (17:0 cyclo) fatty acid accompanied with reduced amounts of unsaturated ones. DCF injures the cells which is expressed by raised activities of acid and alkaline phosphatases as well as formation of lipids peroxidation products (LPX). The elevated activity of superoxide dismutase (SOD) and catalase (CAT) testified that DCF induced oxidative stress. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Piński, A.; Żur, J.; Hasterok, R.; Hupert-Kocurek, K. T.
Comparative genomics of stenotrophomonas maltophilia and stenotrophomonas rhizophila revealed characteristic features of both species Journal Article
In: International Journal of Molecular Sciences, vol. 21, no. 14, pp. 1-20, 2020, ISSN: 16616596, (11).
@article{2-s2.0-85087866006,
title = {Comparative genomics of stenotrophomonas maltophilia and stenotrophomonas rhizophila revealed characteristic features of both species},
author = { A. Piński and J. Żur and R. Hasterok and K.T. Hupert-Kocurek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087866006&doi=10.3390%2fijms21144922&partnerID=40&md5=d3558a7364b4d6e0f87930ba3345b45d},
doi = {10.3390/ijms21144922},
issn = {16616596},
year = {2020},
date = {2020-01-01},
journal = {International Journal of Molecular Sciences},
volume = {21},
number = {14},
pages = {1-20},
publisher = {MDPI AG},
abstract = {Although Stenotrophomonas maltophilia strains are efficient biocontrol agents, their field applications have raised concerns due to their possible threat to human health. The non-pathogenic Stenotrophomonas rhizophila species, which is closely related to S. maltophilia, has been proposed as an alternative. However, knowledge regarding the genetics of S. rhizophila is limited. Thus, the aim of the study was to define any genetic differences between the species and to characterise their ability to promote the growth of plant hosts as well as to enhance phytoremediation efficiency. We compared 37 strains that belong to both species using the tools of comparative genomics and identified 96 genetic features that are unique to S. maltophilia (e.g.; chitin-binding protein; mechanosensitive channels of small conductance and KGG repeat-containing stress-induced protein) and 59 that are unique to S. rhizophila (e.g.; glucosylglycerol-phosphate synthase; cold shock protein with the DUF1294 domain; and pteridine-dependent dioxygenase-like protein). The strains from both species have a high potential for biocontrol, which is mainly related to the production of keratinases (KerSMD and KerSMF), proteinases and chitinases. Plant growth promotion traits are attributed to the biosynthesis of siderophores, spermidine, osmoprotectants such as trehalose and glucosylglycerol, which is unique to S. rhizophila. In eight out of 37 analysed strains, the genes that are required to degrade protocatechuate were present. While our results show genetic differences between the two species, they had a similar growth promotion potential. Considering the information above, S. rhizophila constitutes a promising alternative for S. maltophilia for use in agricultural biotechnology. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {11},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Although Stenotrophomonas maltophilia strains are efficient biocontrol agents, their field applications have raised concerns due to their possible threat to human health. The non-pathogenic Stenotrophomonas rhizophila species, which is closely related to S. maltophilia, has been proposed as an alternative. However, knowledge regarding the genetics of S. rhizophila is limited. Thus, the aim of the study was to define any genetic differences between the species and to characterise their ability to promote the growth of plant hosts as well as to enhance phytoremediation efficiency. We compared 37 strains that belong to both species using the tools of comparative genomics and identified 96 genetic features that are unique to S. maltophilia (e.g.; chitin-binding protein; mechanosensitive channels of small conductance and KGG repeat-containing stress-induced protein) and 59 that are unique to S. rhizophila (e.g.; glucosylglycerol-phosphate synthase; cold shock protein with the DUF1294 domain; and pteridine-dependent dioxygenase-like protein). The strains from both species have a high potential for biocontrol, which is mainly related to the production of keratinases (KerSMD and KerSMF), proteinases and chitinases. Plant growth promotion traits are attributed to the biosynthesis of siderophores, spermidine, osmoprotectants such as trehalose and glucosylglycerol, which is unique to S. rhizophila. In eight out of 37 analysed strains, the genes that are required to degrade protocatechuate were present. While our results show genetic differences between the two species, they had a similar growth promotion potential. Considering the information above, S. rhizophila constitutes a promising alternative for S. maltophilia for use in agricultural biotechnology. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Żur, J.; Michalska, J.; Piński, A.; Mrozik, A.; Nowak, A.
In: Water (Switzerland), vol. 12, no. 4, 2020, ISSN: 20734441, (6).
@article{2-s2.0-85084456097,
title = {Effects of low concentration of selected analgesics and successive bioaugmentation of the activated sludge on its activity and metabolic diversity},
author = { J. Żur and J. Michalska and A. Piński and A. Mrozik and A. Nowak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084456097&doi=10.3390%2fW12041133&partnerID=40&md5=90f5b4c6b8ccf903e458fdb6e230e0c4},
doi = {10.3390/W12041133},
issn = {20734441},
year = {2020},
date = {2020-01-01},
journal = {Water (Switzerland)},
volume = {12},
number = {4},
publisher = {MDPI AG},
abstract = {In this study, we evaluated the impact of the successive bioaugmentation of the activated sludge (AS) with the defined bacterial consortium on the activity and functional capacity of the AS microorganisms. In parallel, the removal of low concentrations of the selected non-steroidal anti-inflammatory drugs (ibuprofen; naproxen; diclofenac) and analgesic paracetamol was studied. We found that the addition of the bacterial consortium consisting of three pharmaceuticals-degrading strains Bacillus thuringiensis B1 (2015b), Stenotrophomonas maltophilia KB2, and Pseudomonas moorei KB4 into the AS did not cause any significant changes in the biomass abundance and metabolic activity of the AS microorganisms. Although, the successive bioaugmentation of the AS caused a slight increase in the metabolic diversity, the intensity of carbohydrates usage, and metabolic richness. Microorganisms in the bioaugmented and non-bioaugmented AS were able to degrade the mixture of the analyzed drugs with similar efficiency, however, diclofenac was removed more effectively in the bioaugmented AS. Several metabolites were identified and efficiently utilized, with the exception of 4-OH diclofenac. Two new diclofenac-degrading strains assigned as Serratia proteamaculans AS4 and Rahnella bruchi AS7 were isolated from the diclofenac-treated AS. © 2020 by the authors.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this study, we evaluated the impact of the successive bioaugmentation of the activated sludge (AS) with the defined bacterial consortium on the activity and functional capacity of the AS microorganisms. In parallel, the removal of low concentrations of the selected non-steroidal anti-inflammatory drugs (ibuprofen; naproxen; diclofenac) and analgesic paracetamol was studied. We found that the addition of the bacterial consortium consisting of three pharmaceuticals-degrading strains Bacillus thuringiensis B1 (2015b), Stenotrophomonas maltophilia KB2, and Pseudomonas moorei KB4 into the AS did not cause any significant changes in the biomass abundance and metabolic activity of the AS microorganisms. Although, the successive bioaugmentation of the AS caused a slight increase in the metabolic diversity, the intensity of carbohydrates usage, and metabolic richness. Microorganisms in the bioaugmented and non-bioaugmented AS were able to degrade the mixture of the analyzed drugs with similar efficiency, however, diclofenac was removed more effectively in the bioaugmented AS. Several metabolites were identified and efficiently utilized, with the exception of 4-OH diclofenac. Two new diclofenac-degrading strains assigned as Serratia proteamaculans AS4 and Rahnella bruchi AS7 were isolated from the diclofenac-treated AS. © 2020 by the authors.
Żur, J.; Piński, A.; Michalska, J.; Hupert-Kocurek, K. T.; Nowak, A.; Wojcieszyńska, D.; Guzik, U.
A whole-cell immobilization system on bacterial cellulose for the paracetamol-degrading Pseudomonas moorei KB4 strain Journal Article
In: International Biodeterioration and Biodegradation, vol. 149, 2020, ISSN: 09648305, (19).
@article{2-s2.0-85079226601,
title = {A whole-cell immobilization system on bacterial cellulose for the paracetamol-degrading Pseudomonas moorei KB4 strain},
author = { J. Żur and A. Piński and J. Michalska and K.T. Hupert-Kocurek and A. Nowak and D. Wojcieszyńska and U. Guzik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079226601&doi=10.1016%2fj.ibiod.2020.104919&partnerID=40&md5=83d05c44cb2a78963ef96e3d769f2088},
doi = {10.1016/j.ibiod.2020.104919},
issn = {09648305},
year = {2020},
date = {2020-01-01},
journal = {International Biodeterioration and Biodegradation},
volume = {149},
publisher = {Elsevier Ltd},
abstract = {Microorganisms with a high natural ability to degrade xenobiotics, which are usually characterized by a diverse metabolism and unique features, can be used as natural biocatalysts in wastewater treatment or bioaugmentation processes. The immobilization of such strains greatly increases their stability and degradation properties. The most critical issues in designing whole-cell immobilization systems are selecting the suitable carrier and determining the bacterial features that will promote effective immobilization. In this study, we immobilized the paracetamol-degrading Pseudomonas moorei KB4 strain on the bacterial cellulose disks that were produced by Komagataeibacter xylinus E-89370. The KB4 strain immobilized on the cellulose degraded 150 mg L−1 of paracetamol in three series of 50 mg L−1 in each cycle. The average protein concentration and dehydrogenase activity increased after the degradation of each dose. The specific activity (U mg−1 of protein) of the main enzymes involved in the degradation pathway was 0.22 for deaminase, 5.1 for acylamidohydrolase and 3.49 for ring-cleavage hydroquinone 1,2-dioxygenase. The relative expression level of the genes encoding deaminases and acylamidohydrolases increased in the presence of paracetamol, though more prominently in the immobilized than in the free cells. © 2020 Elsevier Ltd},
note = {19},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Microorganisms with a high natural ability to degrade xenobiotics, which are usually characterized by a diverse metabolism and unique features, can be used as natural biocatalysts in wastewater treatment or bioaugmentation processes. The immobilization of such strains greatly increases their stability and degradation properties. The most critical issues in designing whole-cell immobilization systems are selecting the suitable carrier and determining the bacterial features that will promote effective immobilization. In this study, we immobilized the paracetamol-degrading Pseudomonas moorei KB4 strain on the bacterial cellulose disks that were produced by Komagataeibacter xylinus E-89370. The KB4 strain immobilized on the cellulose degraded 150 mg L−1 of paracetamol in three series of 50 mg L−1 in each cycle. The average protein concentration and dehydrogenase activity increased after the degradation of each dose. The specific activity (U mg−1 of protein) of the main enzymes involved in the degradation pathway was 0.22 for deaminase, 5.1 for acylamidohydrolase and 3.49 for ring-cleavage hydroquinone 1,2-dioxygenase. The relative expression level of the genes encoding deaminases and acylamidohydrolases increased in the presence of paracetamol, though more prominently in the immobilized than in the free cells. © 2020 Elsevier Ltd
Michalska, J.; Piński, A.; Żur, J.; Mrozik, A.
In: Water (Switzerland), vol. 12, no. 3, 2020, ISSN: 20734441, (9).
@article{2-s2.0-85082558569,
title = {Analysis of the bioaugmentation potential of Pseudomonas putida OR45a and Pseudomonas putida KB3 in the sequencing batch reactors fed with the phenolic landfill leachate},
author = { J. Michalska and A. Piński and J. Żur and A. Mrozik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082558569&doi=10.3390%2fw12030906&partnerID=40&md5=67a6b3c06ffeeb8d282ff6659fcac256},
doi = {10.3390/w12030906},
issn = {20734441},
year = {2020},
date = {2020-01-01},
journal = {Water (Switzerland)},
volume = {12},
number = {3},
publisher = {MDPI AG},
abstract = {The treatment of landfill leachate could be challenging for the biological wastewater treatment systems due to its high toxicity and the presence of poorly biodegradable contaminants. In this study, the bioaugmentation technology was successfully applied in sequencing batch reactors (SBRs) fed with the phenolic landfill leachate by inoculation of the activated sludge (AS) with two phenol-degrading Pseudomonas putida OR45a and Pseudomonas putida KB3 strains. According to the results, the SBRs bioaugmented with Pseudomonas strains withstood the increasing concentrations of the leachate. This resulted in the higher removal efficiency of the chemical oxygen demand (COD) of 79-86%, ammonia nitrogen of 87-88% and phenolic compounds of 85-96% as compared to 45%, 64%, and 50% for the noninoculated SBR. Simultaneously, the bioaugmentation of the AS allowed to maintain the high enzymatic activity of dehydrogenases, nonspecific esterases, and catalase in this ecosystem, which contributed to the higher functional capacity of indigenous microorganisms than in the noninoculated AS. Herein, the stress level experienced by the microorganisms in the SBRs fed with the leachate computed based on the cellular ATP measurements showed that the abundance of exogenous Pseudomonas strains in the bioreactors contributed to the reduction in effluent toxicity, which was reflected by a decrease in the stress biomass index to 32-45% as compared to the nonbioaugmented AS (76%). © 2020 by the authors.},
note = {9},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The treatment of landfill leachate could be challenging for the biological wastewater treatment systems due to its high toxicity and the presence of poorly biodegradable contaminants. In this study, the bioaugmentation technology was successfully applied in sequencing batch reactors (SBRs) fed with the phenolic landfill leachate by inoculation of the activated sludge (AS) with two phenol-degrading Pseudomonas putida OR45a and Pseudomonas putida KB3 strains. According to the results, the SBRs bioaugmented with Pseudomonas strains withstood the increasing concentrations of the leachate. This resulted in the higher removal efficiency of the chemical oxygen demand (COD) of 79-86%, ammonia nitrogen of 87-88% and phenolic compounds of 85-96% as compared to 45%, 64%, and 50% for the noninoculated SBR. Simultaneously, the bioaugmentation of the AS allowed to maintain the high enzymatic activity of dehydrogenases, nonspecific esterases, and catalase in this ecosystem, which contributed to the higher functional capacity of indigenous microorganisms than in the noninoculated AS. Herein, the stress level experienced by the microorganisms in the SBRs fed with the leachate computed based on the cellular ATP measurements showed that the abundance of exogenous Pseudomonas strains in the bioreactors contributed to the reduction in effluent toxicity, which was reflected by a decrease in the stress biomass index to 32-45% as compared to the nonbioaugmented AS (76%). © 2020 by the authors.
Michalska, J.; Piński, A.; Żur, J.; Mrozik, A.
Selecting bacteria candidates for the bioaugmentation of activated sludge to improve the aerobic treatment of landfill leachate Journal Article
In: Water (Switzerland), vol. 12, no. 1, 2020, ISSN: 20734441, (19).
@article{2-s2.0-85079488590,
title = {Selecting bacteria candidates for the bioaugmentation of activated sludge to improve the aerobic treatment of landfill leachate},
author = { J. Michalska and A. Piński and J. Żur and A. Mrozik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079488590&doi=10.3390%2fw12010140&partnerID=40&md5=55858d2eb6135c7439f0d8ad67055693},
doi = {10.3390/w12010140},
issn = {20734441},
year = {2020},
date = {2020-01-01},
journal = {Water (Switzerland)},
volume = {12},
number = {1},
publisher = {MDPI AG},
abstract = {In this study, a multifaceted approach for selecting the suitable candidates for bioaugmentation of activated sludge (AS) that supports leachate treatment was used. To determine the exploitation of 10 bacterial strains isolated from the various matrices for inoculating the AS contaminated with the Kalina pond leachate (KPL), their degradative potential was analyzed along with their aptitude to synthesize compounds improving remediation of pollutants in wastewater and ability to incorporate into the AS flocs. Based on their capability to degrade aromatic compounds (primarily catechol; phenol; and cresols) at a concentration of 1 mg/mL and survive in 12.5% of the KPL, Pseudomonas putida OR45a and P. putida KB3 can be considered to be the best candidates for bioaugmentation of the AS among all of the bacteria tested. Genomic analyses of these two strains revealed the presence of the genes encoding enzymes related to the metabolism of aromatic compounds. Additionally, both microorganisms exhibited a high hydrophobic propensity (above 50%) and an ability to produce biosurfactants as well as high resistance to ammonium (above 600 g/mL) and heavy metals (especially chromium). These properties enable the exploitation of both bacterial strains in the bioremediation of the AS contaminated with the KPL. © 2020 by the authors.},
note = {19},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this study, a multifaceted approach for selecting the suitable candidates for bioaugmentation of activated sludge (AS) that supports leachate treatment was used. To determine the exploitation of 10 bacterial strains isolated from the various matrices for inoculating the AS contaminated with the Kalina pond leachate (KPL), their degradative potential was analyzed along with their aptitude to synthesize compounds improving remediation of pollutants in wastewater and ability to incorporate into the AS flocs. Based on their capability to degrade aromatic compounds (primarily catechol; phenol; and cresols) at a concentration of 1 mg/mL and survive in 12.5% of the KPL, Pseudomonas putida OR45a and P. putida KB3 can be considered to be the best candidates for bioaugmentation of the AS among all of the bacteria tested. Genomic analyses of these two strains revealed the presence of the genes encoding enzymes related to the metabolism of aromatic compounds. Additionally, both microorganisms exhibited a high hydrophobic propensity (above 50%) and an ability to produce biosurfactants as well as high resistance to ammonium (above 600 g/mL) and heavy metals (especially chromium). These properties enable the exploitation of both bacterial strains in the bioremediation of the AS contaminated with the KPL. © 2020 by the authors.
2019
Michalska, J.; Greń, I.; Żur, J.; Wasilkowski, D.; Mrozik, A.
Impact of the biological cotreatment of the Kalina pond leachate on laboratory sequencing batch reactor operation and activated sludge quality Journal Article
In: Water (Switzerland), vol. 11, no. 8, 2019, ISSN: 20734441, (10).
@article{2-s2.0-85070258617,
title = {Impact of the biological cotreatment of the Kalina pond leachate on laboratory sequencing batch reactor operation and activated sludge quality},
author = { J. Michalska and I. Greń and J. Żur and D. Wasilkowski and A. Mrozik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070258617&doi=10.3390%2fw11081539&partnerID=40&md5=54915800dfbf65bd693faf9ec666a830},
doi = {10.3390/w11081539},
issn = {20734441},
year = {2019},
date = {2019-01-01},
journal = {Water (Switzerland)},
volume = {11},
number = {8},
publisher = {MDPI AG},
abstract = {Hauling landfill leachate to offsite urban wastewater treatment plants is a way to achieve pollutant removal. However, the implementation of biological methods for the treatment of landfill leachate can be extremely challenging. This study aims to investigate the effect of blending wastewater with 3.5% and 5.5% of the industrial leachate from the Kalina pond (KPL) on the performance of sequencing batch reactor (SBR) and capacity of activated sludge microorganisms. The results showed that the removal efficiency of the chemical oxygen demand declined in the contaminated SBR from 100% to 69% and, subsequently, to 41% after the cotreatment with 3.5% and 5.5% of the pollutant. In parallel, the activities of the dehydrogenases and nonspecific esterases declined by 58% and 39%, and 79% and 81% after 32 days of the exposure of the SBR to 3.5% and 5.5% of the leachate, respectively. Furthermore, the presence of the KPL in the sewage affected the sludge microorganisms through a reduction in their functional capacity as well as a decrease in the percentages of the marker fatty acids for different microbial groups. A multifactorial analysis of the parameters relevant for the wastewater treatment process confirmed unambiguously the negative impact of the leachate on the operation, activity, and structure of the activated sludge. © 2019 by the authors.},
note = {10},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Hauling landfill leachate to offsite urban wastewater treatment plants is a way to achieve pollutant removal. However, the implementation of biological methods for the treatment of landfill leachate can be extremely challenging. This study aims to investigate the effect of blending wastewater with 3.5% and 5.5% of the industrial leachate from the Kalina pond (KPL) on the performance of sequencing batch reactor (SBR) and capacity of activated sludge microorganisms. The results showed that the removal efficiency of the chemical oxygen demand declined in the contaminated SBR from 100% to 69% and, subsequently, to 41% after the cotreatment with 3.5% and 5.5% of the pollutant. In parallel, the activities of the dehydrogenases and nonspecific esterases declined by 58% and 39%, and 79% and 81% after 32 days of the exposure of the SBR to 3.5% and 5.5% of the leachate, respectively. Furthermore, the presence of the KPL in the sewage affected the sludge microorganisms through a reduction in their functional capacity as well as a decrease in the percentages of the marker fatty acids for different microbial groups. A multifactorial analysis of the parameters relevant for the wastewater treatment process confirmed unambiguously the negative impact of the leachate on the operation, activity, and structure of the activated sludge. © 2019 by the authors.
2018
Żur, J.; Wojcieszyńska, D.; Hupert-Kocurek, K. T.; Marchlewicz, A.; Guzik, U.
Paracetamol – toxicity and microbial utilization. Pseudomonas moorei KB4 as a case study for exploring degradation pathway Journal Article
In: Chemosphere, vol. 206, pp. 192-202, 2018, ISSN: 00456535, (63).
@article{2-s2.0-85048021599,
title = {Paracetamol – toxicity and microbial utilization. Pseudomonas moorei KB4 as a case study for exploring degradation pathway},
author = { J. Żur and D. Wojcieszyńska and K.T. Hupert-Kocurek and A. Marchlewicz and U. Guzik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048021599&doi=10.1016%2fj.chemosphere.2018.04.179&partnerID=40&md5=9941e36fe7554a810b10735fb721bbcc},
doi = {10.1016/j.chemosphere.2018.04.179},
issn = {00456535},
year = {2018},
date = {2018-01-01},
journal = {Chemosphere},
volume = {206},
pages = {192-202},
publisher = {Elsevier Ltd},
abstract = {Paracetamol, a widely used analgesic and antipyretic drug, is currently one of the most emerging pollutants worldwide. Besides its wide prevalence in the literature only several bacterial strains able to degrade this compound have been described. In this study, we isolated six new bacterial strains able to remove paracetamol. The isolated strains were identified as the members of Pseudomonas, Bacillus, Acinetobacter and Sphingomonas genera and characterized phenotypically and biochemically using standard methods. From the isolated strains, Pseudomonas moorei KB4 was able to utilize 50 mg L−1 of paracetamol. As the main degradation products, p-aminophenol and hydroquinone were identified. Based on the measurements of specific activity of acyl amidohydrolase, deaminase and hydroquinone 1,2-dioxygenase and the results of liquid chromatography analyses, we proposed a mechanism of paracetamol degradation by KB4 strain under co-metabolic conditions with glucose. Additionally, toxicity bioassays and the influence of various environmental factors, including pH, temperature, heavy metals at no-observed-effective-concentrations, and the presence of aromatic compounds on the efficiency and mechanism of paracetamol degradation by KB4 strain were determined. This comprehensive study about paracetamol biodegradation will be helpful in designing a treatment systems of wastewaters contaminated with paracetamol. © 2018 Elsevier Ltd},
note = {63},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Paracetamol, a widely used analgesic and antipyretic drug, is currently one of the most emerging pollutants worldwide. Besides its wide prevalence in the literature only several bacterial strains able to degrade this compound have been described. In this study, we isolated six new bacterial strains able to remove paracetamol. The isolated strains were identified as the members of Pseudomonas, Bacillus, Acinetobacter and Sphingomonas genera and characterized phenotypically and biochemically using standard methods. From the isolated strains, Pseudomonas moorei KB4 was able to utilize 50 mg L−1 of paracetamol. As the main degradation products, p-aminophenol and hydroquinone were identified. Based on the measurements of specific activity of acyl amidohydrolase, deaminase and hydroquinone 1,2-dioxygenase and the results of liquid chromatography analyses, we proposed a mechanism of paracetamol degradation by KB4 strain under co-metabolic conditions with glucose. Additionally, toxicity bioassays and the influence of various environmental factors, including pH, temperature, heavy metals at no-observed-effective-concentrations, and the presence of aromatic compounds on the efficiency and mechanism of paracetamol degradation by KB4 strain were determined. This comprehensive study about paracetamol biodegradation will be helpful in designing a treatment systems of wastewaters contaminated with paracetamol. © 2018 Elsevier Ltd
Żur, J.; Piński, A.; Marchlewicz, A.; Hupert-Kocurek, K. T.; Wojcieszyńska, D.; Guzik, U.
Organic micropollutants paracetamol and ibuprofen—toxicity, biodegradation, and genetic background of their utilization by bacteria Journal Article
In: Environmental Science and Pollution Research, vol. 25, no. 22, pp. 21498-21524, 2018, ISSN: 09441344, (90).
@article{2-s2.0-85048822480,
title = {Organic micropollutants paracetamol and ibuprofen—toxicity, biodegradation, and genetic background of their utilization by bacteria},
author = { J. Żur and A. Piński and A. Marchlewicz and K.T. Hupert-Kocurek and D. Wojcieszyńska and U. Guzik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048822480&doi=10.1007%2fs11356-018-2517-x&partnerID=40&md5=bed6633cc8ff497e87e086da67cbead2},
doi = {10.1007/s11356-018-2517-x},
issn = {09441344},
year = {2018},
date = {2018-01-01},
journal = {Environmental Science and Pollution Research},
volume = {25},
number = {22},
pages = {21498-21524},
publisher = {Springer Verlag},
abstract = {Currently, analgesics and nonsteroidal anti-inflammatory drugs (NSAIDs) are classified as one of the most emerging group of xenobiotics and have been detected in various natural matrices. Among them, monocyclic paracetamol and ibuprofen, widely used to treat mild and moderate pain are the most popular. Since long-term adverse effects of these xenobiotics and their biological and pharmacokinetic activity especially at environmentally relevant concentrations are better understood, degradation of such contaminants has become a major concern. Moreover, to date, conventional wastewater treatment plants (WWTPs) are not fully adapted to remove that kind of micropollutants. Bioremediation processes, which utilize bacterial strains with increased degradation abilities, seem to be a promising alternative to the chemical methods used so far. Nevertheless, despite the wide prevalence of paracetamol and ibuprofen in the environment, toxicity and mechanism of their microbial degradation as well as genetic background of these processes remain not fully characterized. In this review, we described the current state of knowledge about toxicity and biodegradation mechanisms of paracetamol and ibuprofen and provided bioinformatics analysis concerning the genetic bases of these xenobiotics decomposition. © 2018, The Author(s).},
note = {90},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Currently, analgesics and nonsteroidal anti-inflammatory drugs (NSAIDs) are classified as one of the most emerging group of xenobiotics and have been detected in various natural matrices. Among them, monocyclic paracetamol and ibuprofen, widely used to treat mild and moderate pain are the most popular. Since long-term adverse effects of these xenobiotics and their biological and pharmacokinetic activity especially at environmentally relevant concentrations are better understood, degradation of such contaminants has become a major concern. Moreover, to date, conventional wastewater treatment plants (WWTPs) are not fully adapted to remove that kind of micropollutants. Bioremediation processes, which utilize bacterial strains with increased degradation abilities, seem to be a promising alternative to the chemical methods used so far. Nevertheless, despite the wide prevalence of paracetamol and ibuprofen in the environment, toxicity and mechanism of their microbial degradation as well as genetic background of these processes remain not fully characterized. In this review, we described the current state of knowledge about toxicity and biodegradation mechanisms of paracetamol and ibuprofen and provided bioinformatics analysis concerning the genetic bases of these xenobiotics decomposition. © 2018, The Author(s).
Jałowiecki, Ł.; Żur, J.; Chojniak, J.; Ejhed, H.; Płaza, G. A.
Properties of Antibiotic-Resistant Bacteria Isolated from Onsite Wastewater Treatment Plant in Relation to Biofilm Formation Journal Article
In: Current Microbiology, vol. 75, no. 5, pp. 639-649, 2018, ISSN: 03438651, (16).
@article{2-s2.0-85040643355,
title = {Properties of Antibiotic-Resistant Bacteria Isolated from Onsite Wastewater Treatment Plant in Relation to Biofilm Formation},
author = { Ł. Jałowiecki and J. Żur and J. Chojniak and H. Ejhed and G.A. Płaza},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040643355&doi=10.1007%2fs00284-017-1428-2&partnerID=40&md5=920aaff518011feea1e626dcc2588b1f},
doi = {10.1007/s00284-017-1428-2},
issn = {03438651},
year = {2018},
date = {2018-01-01},
journal = {Current Microbiology},
volume = {75},
number = {5},
pages = {639-649},
publisher = {Springer New York LLC},
abstract = {The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility. Additionally, biosurfactant production by the isolates was also evaluated. The ability of selected strains to develop a biofilm was assessed by using the crystal violet method, which allows to indirectly quantify the attached bacterial biomass (live; dead cells; and polysaccharides as well). Obtained results showed that 19 of the analyzed strains were able to produce biofilm after 72 h of incubation. The low values of surface tension in the range between 28 and 36 mN/m were observed in the bacteria, which are not able to produce biofilm or be classified as weak biofilm producers. Among biofilm-forming strains the highest autoaggregation index was observed for Mycobacterium brumae and Bacillus alcalophilus. Noteworthy, that some strains capable of biofilm formation showed no aggregation abilities or were characterized by low autoaggregative properties. The results of visual autoaggregation assay showed no visible flocs after given time of incubation. The results from motility test demonstrated that most of the analyzed strains were motile. Noteworthy, that up to now literature data about physiology, biofilm formation, and autoaggregative capabilities of bacteria isolated from onsite wastewater technology are very limited and this paper gives the information on the antibiotic-resistant bacteria with ability to form biofilm. Thus, the present study points to develop novel bioinocula in antibiotic degradation and to reach novel biofilm-dispersing agents produced by various bacteria that can be used as disinfectants or surface-coating agents to prevent microbial surface colonization and biofilm development. © 2018, The Author(s).},
note = {16},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility. Additionally, biosurfactant production by the isolates was also evaluated. The ability of selected strains to develop a biofilm was assessed by using the crystal violet method, which allows to indirectly quantify the attached bacterial biomass (live; dead cells; and polysaccharides as well). Obtained results showed that 19 of the analyzed strains were able to produce biofilm after 72 h of incubation. The low values of surface tension in the range between 28 and 36 mN/m were observed in the bacteria, which are not able to produce biofilm or be classified as weak biofilm producers. Among biofilm-forming strains the highest autoaggregation index was observed for Mycobacterium brumae and Bacillus alcalophilus. Noteworthy, that some strains capable of biofilm formation showed no aggregation abilities or were characterized by low autoaggregative properties. The results of visual autoaggregation assay showed no visible flocs after given time of incubation. The results from motility test demonstrated that most of the analyzed strains were motile. Noteworthy, that up to now literature data about physiology, biofilm formation, and autoaggregative capabilities of bacteria isolated from onsite wastewater technology are very limited and this paper gives the information on the antibiotic-resistant bacteria with ability to form biofilm. Thus, the present study points to develop novel bioinocula in antibiotic degradation and to reach novel biofilm-dispersing agents produced by various bacteria that can be used as disinfectants or surface-coating agents to prevent microbial surface colonization and biofilm development. © 2018, The Author(s).
2017
Jałowiecki, Ł.; Żur, J.; Płaza, G. A.
Norfloxacin degradation by Bacillus subtilis strains able to produce biosurfactants on a bioreactor scale Proceedings
EDP Sciences, vol. 17, 2017, ISSN: 22671242, (4).
@proceedings{2-s2.0-85020240439,
title = {Norfloxacin degradation by Bacillus subtilis strains able to produce biosurfactants on a bioreactor scale},
author = { Ł. Jałowiecki and J. Żur and G.A. Płaza},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020240439&doi=10.1051%2fe3sconf%2f20171700033&partnerID=40&md5=4f633e2645a91e1a59c20d7191ad3e3c},
doi = {10.1051/e3sconf/20171700033},
issn = {22671242},
year = {2017},
date = {2017-01-01},
journal = {E3S Web of Conferences},
volume = {17},
publisher = {EDP Sciences},
abstract = {The discharge of antibiotics into the environment has become a major concern since this group of pharmaceuticals influence on microbial communities not only by its mode of action, but also because of the risk of a worldwide dispersal of antibiotic resistance genes (ARG). Antibiotics residues have been found in various environments such as waters, sediments, and soils. Moreover, most WWTPs are not designed to treat such kind of pollutants, which remain incompletely removed. Currently, biodegradation processes which involved bacterial strains with increased degradation capabilities is one of the most promising technique. The aim of this study was to evaluate the norfloxacin biodegradation potential of the three Bacillus subtilis strains named T-1, T′-1 and I′-1a on a bioreactor scale. The aerobic degradation was conducted in a 5-liter bioreactor on minimal salts medium in co-metabolic culture supplemented with glucose. The degradation rate of norfloxacin was determined with the HPLC technique. The surface tension was determined using ring method in order to observe the changes in biosurfactants production. Also, the biofilm formation abilities of the bacteria with two quantitative methods, crystal violet (CV) method and TTC-based test and enzymes production were evaluated. © The Authors, published by EDP Sciences,2017.},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {proceedings}
}
The discharge of antibiotics into the environment has become a major concern since this group of pharmaceuticals influence on microbial communities not only by its mode of action, but also because of the risk of a worldwide dispersal of antibiotic resistance genes (ARG). Antibiotics residues have been found in various environments such as waters, sediments, and soils. Moreover, most WWTPs are not designed to treat such kind of pollutants, which remain incompletely removed. Currently, biodegradation processes which involved bacterial strains with increased degradation capabilities is one of the most promising technique. The aim of this study was to evaluate the norfloxacin biodegradation potential of the three Bacillus subtilis strains named T-1, T′-1 and I′-1a on a bioreactor scale. The aerobic degradation was conducted in a 5-liter bioreactor on minimal salts medium in co-metabolic culture supplemented with glucose. The degradation rate of norfloxacin was determined with the HPLC technique. The surface tension was determined using ring method in order to observe the changes in biosurfactants production. Also, the biofilm formation abilities of the bacteria with two quantitative methods, crystal violet (CV) method and TTC-based test and enzymes production were evaluated. © The Authors, published by EDP Sciences,2017.
2016
Żur, J.; Wojcieszyńska, D.; Guzik, U.
Metabolic responses of bacterial cells to immobilization Journal Article
In: Molecules, vol. 21, no. 7, 2016, ISSN: 14203049, (93).
@article{2-s2.0-85013041866,
title = {Metabolic responses of bacterial cells to immobilization},
author = { J. Żur and D. Wojcieszyńska and U. Guzik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013041866&doi=10.3390%2fmolecules21070958&partnerID=40&md5=1f7385e8358d6c18fb2becd7c931b2d4},
doi = {10.3390/molecules21070958},
issn = {14203049},
year = {2016},
date = {2016-01-01},
journal = {Molecules},
volume = {21},
number = {7},
publisher = {MDPI AG},
abstract = {In recent years immobilized cells have commonly been used for various biotechnological applications, e.g., antibiotic production, soil bioremediation, biodegradation and biotransformation of xenobiotics in wastewater treatment plants. Although the literature data on the physiological changes and behaviour of cells in the immobilized state remain fragmentary, it is well documented that in natural settings microorganisms are mainly found in association with surfaces, which results in biofilm formation. Biofilms are characterized by genetic and physiological heterogeneity and the occurrence of altered microenvironments within the matrix. Microbial cells in communities display a variety of metabolic differences as compared to their free-living counterparts. Immobilization of bacteria can occur either as a natural phenomenon or as an artificial process. The majority of changes observed in immobilized cells result from protection provided by the supports. Knowledge about the main physiological responses occurring in immobilized cells may contribute to improving the efficiency of immobilization techniques. This paper reviews the main metabolic changes exhibited by immobilized bacterial cells, including growth rate, biodegradation capabilities, biocatalytic efficiency and plasmid stability. © 2016 by the authors; licensee MDPI.},
note = {93},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In recent years immobilized cells have commonly been used for various biotechnological applications, e.g., antibiotic production, soil bioremediation, biodegradation and biotransformation of xenobiotics in wastewater treatment plants. Although the literature data on the physiological changes and behaviour of cells in the immobilized state remain fragmentary, it is well documented that in natural settings microorganisms are mainly found in association with surfaces, which results in biofilm formation. Biofilms are characterized by genetic and physiological heterogeneity and the occurrence of altered microenvironments within the matrix. Microbial cells in communities display a variety of metabolic differences as compared to their free-living counterparts. Immobilization of bacteria can occur either as a natural phenomenon or as an artificial process. The majority of changes observed in immobilized cells result from protection provided by the supports. Knowledge about the main physiological responses occurring in immobilized cells may contribute to improving the efficiency of immobilization techniques. This paper reviews the main metabolic changes exhibited by immobilized bacterial cells, including growth rate, biodegradation capabilities, biocatalytic efficiency and plasmid stability. © 2016 by the authors; licensee MDPI.
Leon-Velarde, C. G.; Happonen, L.; Pajunen, M.; Leskinen, K.; Kropinski, A. M.; Mattinen, L.; Malicka, M.; Żur, J.; Smith, D.; Chen, S.; Nawaz, A.; Johnson, R. P.; Odumeru, J. A.; Griffiths, M. W.; Skurnik, M.
In: Applied and Environmental Microbiology, vol. 82, no. 17, pp. 5340-5353, 2016, ISSN: 00992240, (29).
@article{2-s2.0-84987876550,
title = {Yersinia enterocolitica-specific infection by bacteriophages TG1 and φR1-RT is dependent on temperature-regulated expression of the phage host receptor OmpF},
author = { C.G. Leon-Velarde and L. Happonen and M. Pajunen and K. Leskinen and A.M. Kropinski and L. Mattinen and M. Malicka and J. Żur and D. Smith and S. Chen and A. Nawaz and R.P. Johnson and J.A. Odumeru and M.W. Griffiths and M. Skurnik},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84987876550&doi=10.1128%2fAEM.01594-16&partnerID=40&md5=a4c7a06fc13b13f10d43296355b28c4d},
doi = {10.1128/AEM.01594-16},
issn = {00992240},
year = {2016},
date = {2016-01-01},
journal = {Applied and Environmental Microbiology},
volume = {82},
number = {17},
pages = {5340-5353},
publisher = {American Society for Microbiology},
abstract = {Bacteriophages present huge potential both as a resource for developing novel tools for bacterial diagnostics and for use in phage therapy. This potential is also valid for bacteriophages specific for Yersinia enterocolitica. To increase our knowledge of Y. enterocolitica- specific phages, we characterized two novel yersiniophages. The genomes of the bacteriophages vB_YenM_TG1 (TG1) and vB_YenM_φR1-RT (φR1-RT), isolated from pig manure in Canada and from sewage in Finland, consist of linear double-stranded DNA of 162,101 and 168,809 bp, respectively. Their genomes comprise 262 putative coding sequences and 4 tRNA genes and share 91% overall nucleotide identity. Based on phylogenetic analyses of their whole-genome sequences and large terminase subunit protein sequences, a genus named Tg1virus within the family Myoviridae is proposed, with TG1 and φR1-RT (R1RT in the ICTV database) as member species. These bacteriophages exhibit a host range restricted to Y. enterocolitica and display lytic activity against the epidemiologically significant serotypes O:3, O:5,27, and O:9 at and below 25°C. Adsorption analyses of lipopolysaccharide (LPS) and OmpF mutants demonstrate that these phages use both the LPS inner core heptosyl residues and the outer membrane protein OmpF as phage receptors. Based on RNA sequencing and quantitative proteomics, we also demonstrate that temperature-dependent infection is due to strong repression of OmpF at 37°C. In addition, φR1-RT was shown to be able to enter into a pseudolysogenic state. Together, this work provides further insight into phage-host cell interactions by highlighting the importance of understanding underlying factors which may affect the abundance of phage host receptors on the cell surface. © 2016, American Society for Microbiology.},
note = {29},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Bacteriophages present huge potential both as a resource for developing novel tools for bacterial diagnostics and for use in phage therapy. This potential is also valid for bacteriophages specific for Yersinia enterocolitica. To increase our knowledge of Y. enterocolitica- specific phages, we characterized two novel yersiniophages. The genomes of the bacteriophages vB_YenM_TG1 (TG1) and vB_YenM_φR1-RT (φR1-RT), isolated from pig manure in Canada and from sewage in Finland, consist of linear double-stranded DNA of 162,101 and 168,809 bp, respectively. Their genomes comprise 262 putative coding sequences and 4 tRNA genes and share 91% overall nucleotide identity. Based on phylogenetic analyses of their whole-genome sequences and large terminase subunit protein sequences, a genus named Tg1virus within the family Myoviridae is proposed, with TG1 and φR1-RT (R1RT in the ICTV database) as member species. These bacteriophages exhibit a host range restricted to Y. enterocolitica and display lytic activity against the epidemiologically significant serotypes O:3, O:5,27, and O:9 at and below 25°C. Adsorption analyses of lipopolysaccharide (LPS) and OmpF mutants demonstrate that these phages use both the LPS inner core heptosyl residues and the outer membrane protein OmpF as phage receptors. Based on RNA sequencing and quantitative proteomics, we also demonstrate that temperature-dependent infection is due to strong repression of OmpF at 37°C. In addition, φR1-RT was shown to be able to enter into a pseudolysogenic state. Together, this work provides further insight into phage-host cell interactions by highlighting the importance of understanding underlying factors which may affect the abundance of phage host receptors on the cell surface. © 2016, American Society for Microbiology.