@article{2-s2.0-85049182046,

title = {Prioritization of candidate genes in qtl regions for physiological and biochemical traits underlying drought response in barley (Hordeum vulgare L.)},

author = { K. Gudyś and J. Guzy-Wróbelska and A. Janiak and M.A. Dziurka and A. Ostrowska and K. Hura and B. Jurczyk and K. Żmuda and D. Grzybkowska and J. Śróbka and W. Urban and J. Biesaga-Kościelniak and M. Filek and J. Kościelniak and K. Mikołajczak and P. Ogrodowicz and K. Krystkowiak and A. Kuczyńska and P. Krajewski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049182046&doi=10.3389%2ffpls.2018.00769&partnerID=40&md5=ee4f20bff713e6791812c6e0fb2a84e8},

doi = {10.3389/fpls.2018.00769},

issn = {1664462X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Plant Science},

volume = {9},

publisher = {Frontiers Media S.A.},

abstract = {Drought is one of the most adverse abiotic factors limiting growth and productivity of them were genes encoding antioxidants, carboxylic acid biosynthesis enzymes, heat shock proteins, small auxin up-regulated RNAs, nitric oxide synthase, ATP sulfurylases, and proteins involved in regulation of flowering time. This global approach may be proposed for identification of new CGs that underlies QTLs responsible for complex traits. crops. Among them is barley, ranked fourth cereal worldwide in terms of harvested acreage and production. Plants have evolved various mechanisms to cope with water deficit at different biological levels, but there is an enormous challenge to decipher genes responsible for particular complex phenotypic traits, in order to develop drought tolerant crops. This work presents a comprehensive approach for elucidation of molecular mechanisms of drought tolerance in barley at the seedling stage of development. The study includes mapping of QTLs for physiological and biochemical traits associated with drought tolerance on a high-density function map, projection of QTL confidence intervals on barley physical map, and the retrievement of positional candidate genes (CGs), followed by their prioritization based on Gene Ontology (GO) enrichment analysis. A total of 64 QTLs for 25 physiological and biochemical traits that describe plant water status, photosynthetic efficiency, osmoprotectant and hormone content, as well as antioxidant activity, were positioned on a consensus map, constructed using RIL populations developed from the crosses between European and Syrian genotypes. The map contained a total of 875 SNP, SSR and CGs, spanning 941.86 cM with resolution of 1.1 cM. For the first time, QTLs for ethylene, glucose, sucrose, maltose, raffinose, a-tocopherol, g-tocotrienol content, and catalase activity, have been mapped in barley. Based on overlapping confidence intervals of QTLs, 11 hotspots were identified that enclosedmore than 60%ofmapped QTLs. Genetic and physicalmap integration allowed the identification of 1,101 positional CGs within the confidence intervals of drought response-specific QTLs. Prioritization resulted in the designation of 143 CGs, among them were genes encoding antioxidants, carboxylic acid biosynthesis enzymes, heat shock proteins, small auxin up-regulated RNAs, nitric oxide synthase, ATP sulfurylases, and proteins involved in regulation of flowering time. This global approach may be proposed for identification of new CGs that underlies QTLs responsible for complex traits. © 2018 Gudys, Guzy-Wrobelska, Janiak, Dziurka, Ostrowska, Hura, Jurczyk, Żmuda, Grzybkowska, Śróbka, Urban, Biesaga-Koscielniak, Filek, Koscielniak, Mikołajczak, Ogrodowicz, Krystkowiak, Kuczyńska, Krajewski and Szarejko.},

note = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85043326007,

title = {HorTILLUS—a rich and renewable source of induced mutations for forward/reverse genetics and pre-breeding programs in barley (Hordeumvulgare L.)},

author = { M.E. Szurman-Zubrzycka and J. Zbieszczyk and M. Marzec and J. Jelonek and B. Chmielewska and M.M. Kurowska and M. Krok and A. Daszkowska-Golec and J. Guzy-Wróbelska and D. Gruszka and M. Gajecka and P. Gajewska and M. Stolarek and P. Tylec and P. Sega and S. Lip and M. Kudełko and M. Lorek and M. Gorniak-Walas and A. Malolepszy and N. Podsiadlo and K.P. Szyrajew and A. Keisa and Z. Mbambo and E. Todorowska and M. Gaj and Z. Nita and W. Orlowska-Job and M. Maluszynski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043326007&doi=10.3389%2ffpls.2018.00216&partnerID=40&md5=2be0ee225baa473124ed786053f7b1c1},

doi = {10.3389/fpls.2018.00216},

issn = {1664462X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Plant Science},

volume = {9},

publisher = {Frontiers Media S.A.},

abstract = {TILLING (Targeting Induced Local Lesions IN Genomes) is a strategy used for functional analysis of genes that combines the classical mutagenesis and a rapid, high-throughput identification of mutations within a gene of interest. TILLING has been initially developed as a discovery platform for functional genomics, but soon it has become a valuable tool in development of desired alleles for crop breeding, alternative to transgenic approach. Here we present the HorTILLUS (Hordeum—TILLING—University of Silesia) population created for spring barley cultivar “Sebastian” after double-treatment of seeds with two chemical mutagens: sodium azide (NaN3) and N-methyl-N-nitrosourea (MNU). The population comprises more than 9,600 M2 plants from which DNA was isolated, seeds harvested, vacuum-packed, and deposited in seed bank. M3 progeny of 3,481 M2 individuals was grown in the field and phenotyped. The screening for mutations was performed for 32 genes related to different aspects of plant growth and development. For each gene fragment, 3,072–6,912 M2 plants were used for mutation identification using LI-COR sequencer. In total, 382 mutations were found in 182.2Mb screened. The average mutation density in the HorTILLUS, estimated as 1 mutation per 477kb, is among the highest mutation densities reported for barley. The majority of mutations were G/C to A/T transitions, however about 8% transversions were also detected. Sixty-one percent of mutations found in coding regions were missense, 37.5% silent and 1.1% nonsense. In each gene, the missense mutations with a potential effect on protein function were identified. The HorTILLUS platform is the largest of the TILLING populations reported for barley and best characterized. The population proved to be a useful tool, both in functional genomic studies and in forward selection of barley mutants with required phenotypic changes. We are constantly renewing the HorTILLUS population, which makes it a permanent source of new mutations. We offer the usage of this valuable resource to the interested barley researchers on cooperative basis. © 2018 Szurman-Zubrzycka, Zbieszczyk, Marzec, Jelonek, Chmielewska, Kurowska, Krok, Daszkowska-Golec, Guzy-Wrobelska, Gruszka, Gajecka, Gajewska, Stolarek, Tylec, Sega, Lip, Kudełko, Lorek, Gorniak-Walas, Malolepszy, Podsiadlo, Szyrajew, Keisa, Mbambo, Todorowska, Gaj, Nita, Orlowska-Job, Maluszynski and Szarejko.},

note = {30},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85012876895,

title = {Drought-related secondary metabolites of barley (Hordeum vulgare L.) leaves and their metabolomic quantitative trait loci},

author = { A. Piasecka and A. Sawikowska and A. Kuczyńska and P. Ogrodowicz and K. Mikołajczak and K. Krystkowiak and K. Gudyś and J. Guzy-Wróbelska and P. Krajewski and P. Kachlicki},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85012876895&doi=10.1111%2ftpj.13430&partnerID=40&md5=f30b6f1520310f3830502a8507aa3c7b},

doi = {10.1111/tpj.13430},

issn = {09607412},

year  = {2017},

date = {2017-01-01},

journal = {Plant Journal},

volume = {89},

number = {5},

pages = {898-913},

publisher = {Blackwell Publishing Ltd},

abstract = {Determining the role of plant secondary metabolites in stress conditions is problematic due to the diversity of their structures and the complexity of their interdependence with different biological pathways. Correlation of metabolomic data with the genetic background provides essential information about the features of metabolites. LC-MS analysis of leaf metabolites from 100 barley recombinant inbred lines (RILs) revealed that 98 traits among 135 detected phenolic and terpenoid compounds significantly changed their level as a result of drought stress. Metabolites with similar patterns of change were grouped in modules, revealing differences among RILs and parental varieties at early and late stages of drought. The most significant changes in stress were observed for ferulic and sinapic acid derivatives as well as acylated glycosides of flavones. The tendency to accumulate methylated compounds was a major phenomenon in this set of samples. In addition, the polyamine derivatives hordatines as well as terpenoid blumenol C derivatives were observed to be drought related. The correlation of drought-related compounds with molecular marker polymorphisms resulted in the definition of metabolomic quantitative trait loci in the genomic regions of single-nucleotide polymorphism 3101-111 and simple sequence repeat Bmag0692 with multiple linkages to metabolites. The associations pointed to genes related to the defence response and response to cold, heat and oxidative stress, but not to genes related to biosynthesis of the compounds. We postulate that the significant metabolites have a role as antioxidants, regulators of gene expression and modulators of protein function in barley during drought. © 2016 The Authors. The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology.},

note = {48},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84981288071,

title = {QTLs for earliness and yield-forming traits in the Lubuski × CamB barley RIL population under various water regimes},

author = { P. Ogrodowicz and T. Adamski and K. Mikołajczak and A. Kuczyńska and M. Surma and P. Krajewski and A. Sawikowska and A.G. Górny and K. Gudyś and I. Szarejko and J. Guzy-Wróbelska and K. Krystkowiak},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981288071&doi=10.1007%2fs13353-016-0363-4&partnerID=40&md5=222a3af88cb7ebfde7254ed2aafcdedb},

doi = {10.1007/s13353-016-0363-4},

issn = {12341983},

year  = {2017},

date = {2017-01-01},

journal = {Journal of Applied Genetics},

volume = {58},

number = {1},

pages = {49-65},

publisher = {Springer Verlag},

abstract = {Drought has become more frequent in Central Europe causing large losses in cereal yields, especially of spring crops. The development of new varieties with increased tolerance to drought is a key tool for improvement of agricultural productivity. Material for the study consisted of 100 barley recombinant inbred lines (RILs) (LCam) derived from the cross between Syrian and European parents. The RILs and parental genotypes were examined in greenhouse experiments under well-watered and water-deficit conditions. During vegetation the date of heading, yield and yield-related traits were measured. RIL population was genotyped with microsatellite and single nucleotide polymorphism markers. This population, together with two other populations, was the basis for the consensus map construction, which was used for identification of quantitative trait loci (QTLs) affecting the traits. The studied lines showed a large variability in heading date. It was noted that drought-treatment negatively affected the yield and its components, especially when applied at the flag leaf stage. In total, 60 QTLs were detected on all the barley chromosomes. The largest number of QTLs was found on chromosome 2H. The main QTL associated with heading, located on chromosome 2H (Q.HD.LC-2H), was identified at SNP marker 5880–2547, in the vicinity of Ppd-H1 gene. SNP 5880–2547 was also the closest marker to QTLs associated with plant architecture, spike morphology and grain yield. The present study showed that the earliness allele from the Syrian parent, as introduced into the genome of an European variety could result in an improvement of barley yield performance under drought conditions. © 2016, The Author(s).},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84979255621,

title = {Quantitative trait loci for plant height in Maresi × CamB barley population and their associations with yield-related traits under different water regimes},

author = { K. Mikołajczak and A. Kuczyńska and P. Krajewski and A. Sawikowska and M. Surma and P. Ogrodowicz and T. Adamski and K. Krystkowiak and A.G. Górny and M. Kempa and I. Szarejko and J. Guzy-Wróbelska and K. Gudyś},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979255621&doi=10.1007%2fs13353-016-0358-1&partnerID=40&md5=038dfabd848c74c6be6814c592cf3e42},

doi = {10.1007/s13353-016-0358-1},

issn = {12341983},

year  = {2017},

date = {2017-01-01},

journal = {Journal of Applied Genetics},

volume = {58},

number = {1},

pages = {23-35},

publisher = {Springer Verlag},

abstract = {High-yielding capacity of the modern barley varieties is mostly dependent on the sources of semi-dwarfness associated with the sdw1/denso locus. The objective of the study was to identify quantitative trait loci (QTLs) associated with the plant height and yield potential of barley recombinant inbred lines (RILs) grown under various soil moisture regimes. The plant material was developed from a hybrid between the Maresi (European cv.) and CamB (Syrian cv.). A total of 103 QTLs affecting analysed traits were detected and 36 of them showed stable effects over environments. In total, ten QTLs were found to be significant only under water shortage conditions. Nine QTLs affecting the length of main stem were detected on 2H-6H chromosomes. In four of the detected QTLs, alleles contributed by Maresi had negative effects on that trait, the most significant being the QLSt-3H.1-1 in the 3H.1 linkage group. The close linkage between QTLs identified around the sdw1/denso locus, with positive alleles contributed by Maresi, indicates that the semi-dwarf cv. Maresi could serve as a donor of favourable traits resulting in grain yield improvement, also under water scarcity. Molecular analyses revealed that the Syrian cv. also contributed alleles which increased the yield potential. Available barley resources of genomic annotations were employed to the biological interpretation of detected QTLs. This approach revealed 26 over-represented Gene Ontology terms. In the projected support intervals of QGWSl-5H.3-2 and QLSt-5H.3 on the chromosome 5H, four genes annotated to ‘response to stress’ were found. It suggests that these QTL-regions may be involved in a response of plant to a wide range of environmental disturbances. © 2016, The Author(s).},

note = {26},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84973460147,

title = {Quantitative trait loci for yield and yield-related traits in spring barley populations derived from crosses between European and Syrian cultivars},

author = { K. Mikołajczak and P. Ogrodowicz and K. Gudyś and K. Krystkowiak and A. Sawikowska and W. Frohmberg and A.G. Górny and A. Kedziora and J. Jankowiak and D. Józefczyk and G. Karg and J. Andrusiak and P. Krajewski and I. Szarejko and M. Surma and T. Adamski and J. Guzy-Wróbelska and A. Kuczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84973460147&doi=10.1371%2fjournal.pone.0155938&partnerID=40&md5=e12e44fddaf0bc9bb34cf48ac0169ee5},

doi = {10.1371/journal.pone.0155938},

issn = {19326203},

year  = {2016},

date = {2016-01-01},

journal = {PLoS ONE},

volume = {11},

number = {5},

publisher = {Public Library of Science},

abstract = {In response to climatic changes, breeding programmes should be aimed at creating new cultivars with improved resistance to water scarcity. The objective of this study was to examine the yield potential of barley recombinant inbred lines (RILs) derived from three cross-combinations of European and Syrian spring cultivars, and to identify quantitative trait loci (QTLs) for yield-related traits in these populations. RILs were evaluated in field experiments over a period of three years (2011 to 2013) and genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers; a genetic map for each population was constructed and then one consensus map was developed. Biological interpretation of identified QTLs was achieved by reference to Ensembl Plants barley gene space. Twelve regions in the genomes of studied RILs were distinguished after QTL analysis. Most of the QTLs were identified on the 2H chromosome, which was the hotspot region in all three populations. Syrian parental cultivars contributed alleles decreasing traits' values at majority of QTLs for grain weight, grain number, spike length and time to heading, and numerous alleles increasing stem length. The phenomic and molecular approaches distinguished the lines with an acceptable grain yield potential combining desirable features or alleles from their parents, that is, early heading from the Syrian breeding line (Cam/B1/CI08887//CI05761) and short plant stature from the European semidwarf cultivar (Maresi). © 2016 Mikołajczak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {34},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84927173271,

title = {Morphological, genetic and molecular characteristics of barley root hair mutants},

author = { B. Chmielewska and A. Janiak and J. Karcz and J. Guzy-Wróbelska and B.P. Forster and M. Nawrot and A. Rusek and P. Smyda and P. Kędziorski and M. Maluszynski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927173271&doi=10.1007%2fs13353-014-0225-x&partnerID=40&md5=6c3835a594fbf5122995a378c846e7ad},

doi = {10.1007/s13353-014-0225-x},

issn = {12341983},

year  = {2014},

date = {2014-01-01},

journal = {Journal of Applied Genetics},

volume = {55},

number = {4},

pages = {433-447},

publisher = {Springer Verlag},

abstract = {Root hairs are tubular outgrowths of specialized epidermal cells called trichoblasts. They affect anchoring plants in soil, the uptake of water and nutrients and are the sites of the interaction between plants and microorganisms. Nineteen root hair mutants of barley representing different stages of root hair development were subjected to detailed morphological and genetic analyses. Each mutant was monogenic and recessive. An allelism test revealed that nine loci were responsible for the mutated root hair phenotypes in the collection and 1–4 mutated allelic forms were identified at each locus. Genetic relationships between the genes responsible for different stages of root hair formation were established. The linkage groups of four loci rhl1, rhp1, rhi1 and rhs1, which had previously been mapped on chromosomes 7H, 1H, 6H and 5H, respectively, were enriched with new markers that flank the genes at a distance of 0.16 cM to 4.6 cM. The chromosomal position of three new genes – two that are responsible for the development of short root hairs (rhs2 and rhs3) and the gene that controls an irregular root hair pattern (rhi2) – were mapped on chromosomes 6H, 2H and 1H, respectively. A comparative analysis of the agrobotanical parameters between some mutants and their respective parental lines showed that mutations in genes responsible for root hair development had no effect on the agrobotanical performance of plants that were grown under controlled conditions. The presented mutant collection is a valuable tool for further identification of genes controlling root hair development in barley. © The Author(s) 2014},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84885341442,

title = {Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence},

author = { K. Houston and S.M. McKim and J. Comadran and N. Bonar and I. Druka and N. Uzrek and E. Cirillo and J. Guzy-Wróbelska and N.C. Collins and C. Halpin and M. Hansson and C. Dockter and A. Druka and R. Waugha},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84885341442&doi=10.1073%2fpnas.1311681110&partnerID=40&md5=83038a5e3be006ba9790538718752661},

doi = {10.1073/pnas.1311681110},

issn = {00278424},

year  = {2013},

date = {2013-01-01},

journal = {Proceedings of the National Academy of Sciences of the United States of America},

volume = {110},

number = {41},

pages = {16675-16680},

abstract = {Within the cereal grasses, variation in inflorescence architecture results in a conspicuous morphological diversity that in crop species influences the yield of cereal grains. Although significant progress has been made in identifying some of the genes underlying this variation in maize and rice, in the temperate cereals, a group that includes wheat, barley, and rye, only the dosagedependent and highly pleiotropic Q locus in hexaploid wheat has been molecularly characterized. Here we show that the characteristic variation in the density of grains along the inflorescence, or spike, of modern cultivated barley (Hordeum vulgare) is largely the consequence of a perturbed interaction between microRNA172 and its corresponding binding site in the mRNA of an APELATA2 (AP2)- like transcription factor, HvAP2. We used genome-wide association and biparental mapping to identify HvAP2. By comparing inflorescence development and HvAP2 transcript abundance in an extreme dense-spike mutant and its nearly isogenic WT line, we show that HvAP2 turnover driven by microRNA 172 regulates the length of a critical developmental window that is required for elongation of the inflorescence internodes. Our data indicate that this heterochronic change, an altered timing of developmental events caused by specific temporal variation in the efficiency of HvAP2 turnover, leads to the striking differences in the size and shape of the barley spike.},

note = {97},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84874022211,

title = {Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed},

author = { J. Guzy-Wróbelska and M. Filek and A. Kaliciak and I. Szarejko and I. Macháčková and J. Krekule and M. Barciszewska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874022211&doi=10.1007%2fs11738-012-1126-4&partnerID=40&md5=795dc94a2515a119691d6d0b58e87e04},

doi = {10.1007/s11738-012-1126-4},

issn = {01375881},

year  = {2013},

date = {2013-01-01},

journal = {Acta Physiologiae Plantarum},

volume = {35},

number = {3},

pages = {817-827},

publisher = {Polish Academy of Sciences, Institute of Slavic Studies},

abstract = {Vernalization-induced flowering is an effect of the epigenetic regulation of gene expression through DNA methylation and histone modifications. Vernalization-mediated silencing of a floral repressor through histone modifications was shown in Arabidopsis thaliana. However, for Brassica napus L., the mechanism underlying vernalization is unclear, and the roles of DNA methylation and histone modifications have not been established. This study revealed the profiles of changes in the DNA methylation state during vernalization (after 14; 35; 56 days) and the subsequent growth in long- or short-day photoperiods (after 2; 7; 14 days) in the winter and spring rapeseed using TLC and MSAP techniques. TLC analysis showed a significant decrease in the amount of 5-methylcytosine (m5C) in genomic DNA in both cultivars at the beginning of vernalization, but upon its termination, the winter rape showed a reduced level of m5C contrary to a significantly increased level in the spring rape. MSAP analysis revealed that winter and spring rapeseed differed in the MSAP loci which were demethylated/methylated in the course of the experiment and presented diverse profiles of changes in the methylation state. The winter rape showed permanent demethylations at 69. 2 % of MSAP loci in the course of vernalization that were mostly preserved upon its termination. The spring rape showed similar numbers of demethylations and methylations that were mainly transient. The study provides evidence of the role of DNA methylation in vernalization for rapeseed and for the significant prevalence of demethylations at the beginning of vernalization, which is necessary for the transition to reproductive growth. © 2012 The Author(s).},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77953259907,

title = {Exploiting induced variation to dissect quantitative traits in barley},

author = { A. Druka and J. Franckowiak and U. Lundqvist and N. Bonar and J. Alexander and J. Guzy-Wróbelska and L. Ramsay and I. Druka and I. Grant and M. Macaulay and V. Vendramin and F. Shahinnia and S. Radovic and K. Houston and D. Harrap and L. Cardle and D. Marshall and M. Morgante and N. Stein and R. Waugh},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953259907&doi=10.1042%2fBST0380683&partnerID=40&md5=ee51ec8d7aef8e3494dcbe2efc58b596},

doi = {10.1042/BST0380683},

issn = {03005127},

year  = {2010},

date = {2010-01-01},

journal = {Biochemical Society Transactions},

volume = {38},

number = {2},

pages = {683-688},

abstract = {The identification of genes underlying complex quantitative traits such as grain yield by means of conventional genetic analysis (positional cloning) requires the development of several large mapping populations. However, it is possible that phenotypically related, butmore extreme, allelic variants generated by mutational studies could provide a means for more efficient cloning of QTLs (quantitative trait loci). In barley (Hordeum vulgare), with the development of high-throughput genome analysis tools, efficient genome-wide identification of genetic loci harbouring mutant alleles has recently become possible. Genotypic data from NILs (near-isogenic lines) that carry induced or natural variants of genes that control aspects of plant development can be compared with the location of QTLs to potentially identify candidate genes for development-related traits such as grain yield. As yield itself can be divided into a number of allometric component traits such as tillers per plant, kernels per spike and kernel size, mutant alleles that both affect these traits and are located within the confidence intervals for major yield QTLs may represent extreme variants of the underlying genes. In addition, the development of detailed comparative genomic models based on the alignment of a high-density barley gene map with the rice and sorghum physical maps, has enabled an informed prioritization of 'known function' genes as candidates for both QTLs and induced mutant genes. ©The Authors.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34249037847,

title = {Different recombination frequencies in wheat doubled haploid populations obtained through maize pollination and anther culture},

author = { J. Guzy-Wróbelska and A. Labocha-Pawłowska and M. Kwaśniewski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34249037847&doi=10.1007%2fs10681-007-9364-z&partnerID=40&md5=0eb44c88716036d4377367ab39d9ccea},

doi = {10.1007/s10681-007-9364-z},

issn = {00142336},

year  = {2007},

date = {2007-01-01},

journal = {Euphytica},

volume = {156},

number = {1-2},

pages = {173-183},

abstract = {This study compared the meiotic recombination frequency between wheat doubled haploid (DH) populations obtained through two different methods, maize pollination (MP♀) and anther culture (AC♂). The comparison was based on a genetic linkage analysis, performed with DNA markers. Thirty-five polymorphic markers (15 SSR; 15 AFLP; 5 RAPD) were screened in MP♀ and AC♂ doubled haploids populations, derived from the same hybrid genotype (F1 of 'Eta' x 'Darkhan 15'). Nine linkage groups, comprising 35 loci (the MP♀ lines) and 31 loci (the AC♂ lines), were constructed. The linkage groups in both DH populations showed identical orders of markers, except for one group mapping to chromosome 6B. The MP♀ and AC♂ linkage maps differed significantly in recombination frequencies for corresponding intervals. In total, the AC♂ linkage map (495.5 cM) was 40.5% longer than the MP♀ map (352.8 cM), indicating a significantly higher meiotic recombination rate in pollen mother cells. The enhancement in recombination was visible in five of nine linkage groups, and in 7 intervals between individual loci out of 19 compared. Moreover, for 6 other intervals a lack of linkage was observed in the AC♂ population, as compared to the MP♀ map. © 2007 Springer Science+Business Media B.V.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0042853475,

title = {Molecular and agronomic evaluation of wheat doubled haploid lines obtained through maize pollination and anther culture methods},

author = { J. Guzy-Wróbelska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042853475&doi=10.1046%2fj.1439-0523.2003.00858.x&partnerID=40&md5=1fad8dffb8d6c2c45a963eb0edb66741},

doi = {10.1046/j.1439-0523.2003.00858.x},

issn = {01799541},

year  = {2003},

date = {2003-01-01},

journal = {Plant Breeding},

volume = {122},

number = {4},

pages = {305-313},

abstract = {Although maize pollination (MP) and anther culture (AC) are alternative techniques widely used for wheat doubled haploid (DH) production, there is only limited information on the attributes of the plant materials produced through both methods. This study was conducted to evaluate genetic fidelity, transmission of parental gametes, and to compare field performance of DH populations produced by the MP and AC methods from the F1s of two crosses between spring bread wheat cultivars. The DH populations were compared to single seed descent (SSD) lines created from the same crosses. In total, 76 MP and 122 AC lines of the cross between cultivars of divergent origin were subjected to RAPD and AFLP analysis. Only changes in AFLP banding patterns, at similarly low frequencies, 0.18% (MP) and 0.21% (AC), were detected. The frequency of the DH lines affected by the variation, 14.5% (MP) and 14.8% (AC), was similar in both populations. For most of the DH lines, variation in 1-2 loci only, out of several hundreds scored, was observed. A total of 14.3% (MP) and 22.2% (AC) marker loci showed the significant segregation distortion from the expected 1:1 ratio, but in at least one polymorphic locus the within-cultivar variation was responsible for the skewed segregation. The field performance of the corresponding MP and AC lines derived from two crosses confirmed the equivalency of both DH populations. In most of the traits analyzed, the MP and AC lines performed the same as the SSD populations created from the same crosses. No, or very small differences in means and ranges, were observed when the best 10% of the lines from all three methods were compared. Moreover, the best 10% of the lines of the cross between Polish wheat cultivars adapted to the local environment performed significantly better for some traits than different groups of checks used in the study.},

note = {28},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-0036270608,

title = {The origin of species of Acer (Sapindaceae) endemic to Ullung Island, Korea},

author = { M.F. Pfosser and J. Guzy-Wróbelska and B.Y. Sun and T.F. Stuessy and T. Sugawara and N. Fujii},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036270608&partnerID=40&md5=04b525726f49196db00bcb4c364c6e2b},

issn = {03636445},

year  = {2002},

date = {2002-01-01},

journal = {Systematic Botany},

volume = {27},

number = {2},

pages = {351-367},

abstract = {We have conducted a phylogenetic analysis of 57 taxa including 127 accessions of Acer to determine the geographical origin and the presumptive progenitor species of Acer takesimense and A. okamotoanum, two endemics of the Korean island Ullung-do. Sequence analyses of the trnL intron and the trnL-trnF intergenic spacer of chloroplast DNA show a close relationship of A. takesimense to A. pseudosieboldianum from mainland Korea. Acer okamotoanum probably has evolved from Korean individuals of A. mono and not from Japan where A. mono is also native. AFLP data further support the proposed relationships and indicate a substantial loss of genetic diversity of A. takesimense when compared with the progenitor populations from mainland Korea. Both sequence and AFLP data put A. takesimense populations into a monophyletic group, suggesting that they speciated in Ullung Island from a single introduction from Korean peninsular populations. Populations of A. okamotoanum, on the other hand, show a higher degree of shared alleles with at least two mainland populations of their presumptive progenitor A. mono, perhaps indicating multiple origins of the latter endemic species.},

note = {41},

keywords = {},

pubstate = {published},

tppubtype = {article}

}