@article{2-s2.0-85049247123,

title = {Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.)},

author = { E. Skrzypek and T. Warzecha and A. Noga and M. Warchoł and I. Czyczyło-Mysza and K. Dziurka and I. Marcińska and K. Kapłoniak and A. Sutkowska and Z. Nita and K. Werwińska and D. Idziak-Helmcke and M. Rojek-Jelonek and M. Hosiawa-Barańska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049247123&doi=10.7717%2fpeerj.5107&partnerID=40&md5=0325cd81ea9a5e47f329ef405e3a02ce},

doi = {10.7717/peerj.5107},

issn = {21678359},

year  = {2018},

date = {2018-01-01},

journal = {PeerJ},

volume = {2018},

number = {6},

publisher = {PeerJ Inc.},

abstract = {Background. The oat×maize addition (OMA) lines are used for mapping of the maize genome, the studies of centromere-specific histone (CENH3), gene expression, meiotic chromosome behavior and also for introducing maize C4 photosynthetic system to oat. The aim of our study was the identification and molecular-cytogenetic characterization of oat × maize hybrids. Methods. Oat DH lines and oat × maize hybrids were obtained using the wide crossing of Avena sativa L. with Zea mays L. The plants identified as having a Grande- 1 retrotransposon fragment, which produced seeds, were used for genomic in situ hybridization (GISH). Results. A total of 138 oat lines obtained by crossing of 2,314 oat plants from 80 genotypes with maize cv. Waza were tested for the presence of maize chromosomes. The presence of maize chromatin was indicated in 66 lines by amplification of the PCR product (500 bp) generated using primers specific for the maize retrotransposon Grande-1. Genomic in situ hybridization (GISH) detected whole maize chromosomes in eight lines (40%). All of the analyzed plants possessed full complement of oat chromosomes. The number of maize chromosomes differed between the OMA lines. Four OMA lines possessed two maize chromosomes similar in size, three OMA-one maize chromosome, and one OMA-four maize chromosomes. In most of the lines, the detected chromosomes were labeled uniformly. The presence of six 45S rDNA loci was detected in oat chromosomes, but none of the added maize chromosomes in any of the lines carried 45S rDNA locus. Twenty of the analyzed lines did not possess whole maize chromosomes, but the introgression of maize chromatin in the oat chromosomes. Five of 66 hybrids were shorter in height, grassy type without panicles. Twenty-seven OMA lines were fertile and produced seeds ranging in number from 1-102 (in total 613). Sixty-three fertile DH lines, out of 72 which did not have an addition of maize chromosomes or chromatin, produced seeds in the range of 1-343 (in total 3;758). Obtained DH and OMA lines were fertile and produced seeds. Discussion. In wide hybridization of oat with maize, the complete or incomplete chromosomes elimination of maize occur. Hybrids of oat and maize had a complete set of oat chromosomes without maize chromosomes, and a complete set of oat chromosomes with one to four retained maize chromosomes. © 2018 Skrzypek et al.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85010866457,

title = {Evolutional dynamics of 45S and 5S ribosomal DNA in ancient allohexaploid Atropa belladonna},

author = { R.A. Volkov and I.I. Panchuk and N.V. Borisjuk and M. Hosiawa-Barańska and J. Małuszyńska and V. Hemleben},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010866457&doi=10.1186%2fs12870-017-0978-6&partnerID=40&md5=9029274aa0994b70e3e6ce6686820fa5},

doi = {10.1186/s12870-017-0978-6},

issn = {14712229},

year  = {2017},

date = {2017-01-01},

journal = {BMC Plant Biology},

volume = {17},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: Polyploid hybrids represent a rich natural resource to study molecular evolution of plant genes and genomes. Here, we applied a combination of karyological and molecular methods to investigate chromosomal structure, molecular organization and evolution of ribosomal DNA (rDNA) in nightshade, Atropa belladonna (fam. Solanaceae), one of the oldest known allohexaploids among flowering plants. Because of their abundance and specific molecular organization (evolutionarily conserved coding regions linked to variable intergenic spacers; IGS), 45S and 5S rDNA are widely used in plant taxonomic and evolutionary studies. Results: Molecular cloning and nucleotide sequencing of A. belladonna 45S rDNA repeats revealed a general structure characteristic of other Solanaceae species, and a very high sequence similarity of two length variants, with the only difference in number of short IGS subrepeats. These results combined with the detection of three pairs of 45S rDNA loci on separate chromosomes, presumably inherited from both tetraploid and diploid ancestor species, example intensive sequence homogenization that led to substitution/elimination of rDNA repeats of one parent. Chromosome silver-staining revealed that only four out of six 45S rDNA sites are frequently transcriptionally active, demonstrating nucleolar dominance. For 5S rDNA, three size variants of repeats were detected, with the major class represented by repeats containing all functional IGS elements required for transcription, the intermediate size repeats containing partially deleted IGS sequences, and the short 5S repeats containing severe defects both in the IGS and coding sequences. While shorter variants demonstrate increased rate of based substitution, probably in their transition into pseudogenes, the functional 5S rDNA variants are nearly identical at the sequence level, pointing to their origin from a single parental species. Localization of the 5S rDNA genes on two chromosome pairs further supports uniparental inheritance from the tetraploid progenitor. Conclusions: The obtained molecular, cytogenetic and phylogenetic data demonstrate complex evolutionary dynamics of rDNA loci in allohexaploid species of Atropa belladonna. The high level of sequence unification revealed in 45S and 5S rDNA loci of this ancient hybrid species have been seemingly achieved by different molecular mechanisms. © 2017 The Author(s).},

note = {39},

keywords = {},

pubstate = {published},

tppubtype = {article}

}