@article{2-s2.0-85184514939,

title = {The gain-of-function mutation blf13 in the barley orthologue of the rice growth regulator NARROW LEAF1 is associated with increased leaf width},

author = { M. Jöst and O. Soltani and C.D. Kappel and A. Janiak and B. Chmielewska and M.E. Szurman-Zubrzycka and S.M. McKim and M. Lenhard},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184514939&doi=10.1093%2fjxb%2ferad403&partnerID=40&md5=73b5c97b4125650f5df4755fd740378f},

doi = {10.1093/jxb/erad403},

year  = {2024},

date = {2024-01-01},

journal = {Journal of Experimental Botany},

volume = {75},

number = {3},

pages = {850-867},

publisher = {Oxford University Press},

abstract = {Canopy architecture in cereals plays an important role in determining yield. Leaf width represents one key aspect of this canopy architecture. However, our understanding of leaf width control in cereals remains incomplete. Classical mutagenesis studies in barely identified multiple morphological mutants, including those with differing leaf widths. Of these, we characterized the broad leaf13 (blf13) mutant in detail. Mutant plants form wider leaves due to increased post-initiation growth and cell proliferation. The mutant phenotype perfectly co-segregated with a missense mutation in the HvHNT1 gene which affected a highly conserved region of the encoded protein, orthologous to the rice NARROW LEAF1 (NAL1) protein. Causality of this mutation for the blf13 phenotype is further supported by correlative transcriptomic analyses and protein-protein interaction studies showing that the mutant HvNHT1 protein interacts more strongly with a known interactor than wild-type HvHNT1. The mutant HvHNT1 protein also showed stronger homodimerization compared with wild-type HvHNT1, and homology modelling suggested an additional interaction site between HvHNT1 monomers due to the blf13 mutation. Thus, the blf13 mutation parallels known gain-of-function NAL1 alleles in rice that increase leaf width and grain yield, suggesting that the blf13 mutation may have a similar agronomic potential in barley. © 2023 The Author(s). Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85106728705,

title = {Identification of novel plant architecture mutants in barley},

author = { S. Mohammadi Aghdam and B. Abdollahi Mandoulakani and L. Rossini and A. Janiak and S. Shaaf},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106728705&doi=10.1007%2fs42976-021-00178-6&partnerID=40&md5=2880839b343519cd99ac2e8a512bd9b5},

doi = {10.1007/s42976-021-00178-6},

issn = {01333720},

year  = {2022},

date = {2022-01-01},

journal = {Cereal Research Communications},

volume = {50},

number = {2},

pages = {179-189},

publisher = {Akademiai Kiado ZRt.},

abstract = {In grasses, biomass and grain production are affected by plant architecture traits such as tiller number, leaf size and orientation. Thus, knowledge regarding their genetic basis is a prerequisite for developing new improved varieties. Mutant screens represent a powerful approach to identify genetic factors underpinning these traits: the HorTILLUS population, obtained by mutagenesis of spring two-row cultivar Sebastian, is a valuable resource for this purpose in barley. In this study, 20 mutant families from the HorTILLUS population were selected and evaluated for tiller number, leaf angle and a range of other plant architecture and agronomic traits using an unreplicated field design with Sebastian as a check cultivar. Principal Component Analysis revealed strong relationships among number of tillers, upper canopy leaf angle, biomass and yield-related traits. Comparison to the Sebastian background revealed that most mutants significantly differed from the wild-type for multiple traits, including two mutants with more erect leaves and four mutants with increased tiller number in at least one phenological stage. Heatmap clustering identified two main groups: the first containing the two erect mutants and the second containing Sebastian and the high-tillering mutants. Among the high-tillering mutants, two showed significantly higher biomass and grain yield per plant compared to Sebastian. The selected mutants represent promising materials for the identification of genetic factors controlling tillering and leaf angle in barley. © 2021, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85123626788,

title = {The barley mutant happy under the sun 1 (hus1): An additional contribution to pale green crops},

author = { L. Rotasperti and L. Tadini and M. Chiara and C. Crosatti and D. Guerra and A. Tagliani and S. Forlani and I. Ezquer and D.S. Horner and P. Jahns and K. Gajek and A. García and R. Savin and L. Rossini and A. Tondelli and A. Janiak and P. Pesaresi},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123626788&doi=10.1016%2fj.envexpbot.2022.104795&partnerID=40&md5=4f093499bc9916285ba25ed8e6d639c7},

doi = {10.1016/j.envexpbot.2022.104795},

issn = {00988472},

year  = {2022},

date = {2022-01-01},

journal = {Environmental and Experimental Botany},

volume = {196},

publisher = {Elsevier B.V.},

abstract = {Truncated antenna size of photosystems and lower leaf chlorophyll content has been shown to increase photosynthetic efficiency and biomass accumulation in microalgae, cyanobacteria and higher plants grown under high-density cultivation conditions. Here, we have asked whether this strategy is also applicable to a major crop by characterizing the barley mutant happy under the sun 1 (hus1). The pale green phenotype of hus1 is due to a 50% reduction in the chlorophyll content of leaves, owing to a premature stop codon in the HvcpSRP43 gene for the 43-kDa chloroplast Signal Recognition Particle (cpSRP43). The HvcpSRP43 protein is responsible for the uploading of photosystem antenna proteins into the thylakoid membranes, and its truncation results in a smaller photosystem antenna size. Besides a detailed molecular and physiological characterization of the mutant grown under controlled greenhouse conditions, we show that the agronomic performance of hus1 plants, in terms of total biomass production and grain yield under standard field conditions, is comparable to that of control plants. The results are discussed in terms of the potential benefits of the hus1 phenotype, and of natural allelic variants of the HvcpSRP43 locus, with respect to productivity and mitigation of climate change. © 2022 Elsevier B.V.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85121336640,

title = {Whole exome sequencing-based identification of a novel gene involved in root hair development in barley (Hordeum vulgare l.)},

author = { K. Gajek and A. Janiak and U. Korotko and B. Chmielewska and M. Marzec and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121336640&doi=10.3390%2fijms222413411&partnerID=40&md5=0d73d429a527fcb38849ebab9bd1fe43},

doi = {10.3390/ijms222413411},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {24},

publisher = {MDPI},

abstract = {Root hairs play a crucial role in anchoring plants in soil, interaction with microorganisms and nutrient uptake from the rhizosphere. In contrast to Arabidopsis, there is a limited knowledge of root hair morphogenesis in monocots, including barley (Hordeum vulgare L.). We have isolated barley mutant rhp1.e with an abnormal root hair phenotype after chemical mutagenesis of spring cultivar ‘Sebastian’. The development of root hairs was initiated in the mutant but inhibited at the very early stage of tip growth. The length of root hairs reached only 3% of the length of parent cultivar. Using a whole exome sequencing (WES) approach, we identified G1674A mutation in the HORVU1Hr1G077230 gene, located on chromosome 1HL and encoding a cellulose synthase-like C1 protein (HvCSLC1) that might be involved in the xyloglucan (XyG) synthesis in root hairs. The identified mutation led to the retention of the second intron and premature termination of the HvCSLC1 protein. The mutation co-segregated with the abnormal root hair phenotype in the F2 progeny of rhp1.e mutant and its wild-type parent. Additionally, different substitutions in HORVU1Hr1G077230 were found in four other allelic mutants with the same root hair phenotype. Here, we discuss the putative role of HvCSLC1 protein in root hair tube elongation in barley. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85114441256,

title = {Significance of selenium supplementation in root- shoot reactions under manganese stress in wheat seedlings – biochemical and cytological studies},

author = { A. Sieprawska and M. Skórka and E. Bednarska-Kozakiewicz and K. Niedojadło and A. Janiak and A. Telk and M. Filek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114441256&doi=10.1007%2fs11104-021-05138-x&partnerID=40&md5=c6b913c4bf6a44ae014b7c5d9f7db694},

doi = {10.1007/s11104-021-05138-x},

issn = {0032079X},

year  = {2021},

date = {2021-01-01},

journal = {Plant and Soil},

volume = {468},

number = {1-2},

pages = {389-410},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Purpose: Agronomic practices are one of the reasons for the increasing accumulation of elements in the soil, including manganese (Mn). Our previous studies have shown that selenium (Se) ions can reduce the toxic actions of metal stress. Those, we studied the effects of Mn—treated as a stressor and Se – as a potential defense in plants. Methods: Mn ions (10 mM) or/and Se (15 μM) were added into hydroponic nutrients of two wheat cultivares. The evaluation of the stress-generating and protective actions were analyzed by biochemical methods and microscopic observations in leaves and roots. Moreover the level of DNA methylation for these tissues was determined. Results: Mn application caused an increase of lipid peroxidation and hydrogen peroxide content in both leaves and roots and was accompanied with a greater absorption of this element by the roots. For other elements (K; Fe; S; P), with the exception of Ca, the reduced their uptake was registered, especially in roots. For roots, Mn stimulated greater, microscopically observed, desorganization in cell structure as compared to leaves, which was accompanied by a quantitative increase in 5-methylcytosine (5-metC) in root meristem. Se application diminished the effects of Mn-stress. Conclusions: These studies is the first in which indicated that global 5-metC level in roots enhancing from dividing meristematic cells to elongating cells of the axial cylinder and cortex. It was suggested that the rise in Ca level can lead to modification of root cells differentiations what may be one of the steps in defense mechanisms. © 2021, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85076778671,

title = {Genetic and physiological dissection of photosynthesis in barley exposed to drought stress},

author = { A. Daszkowska-Golec and A. Collin and K. Sitko and A. Janiak and H.M. Kalaji and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076778671&doi=10.3390%2fijms20246341&partnerID=40&md5=14faebbe60c567c85947758707f74195},

doi = {10.3390/ijms20246341},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {24},

publisher = {MDPI AG},

abstract = {Balanced photosynthesis under drought is essential for better survival and for agricultural benefits in terms of biomass and yield. Given the current attempts to improve the photosynthetic efficiency for greater crop yield, the explanation of the genetic basis of that process, together with the phenotypic analysis, is significant in terms of both basic studies and potential agricultural application. Therefore, the main objective of this study was to uncover the molecular basis of the photosynthesis process under drought stress in barley. To address that goal, we conducted transcriptomic examination together with detailed photosynthesis analysis using the JIP-test. Using this approach, we indicated that photosynthesis is a process that is very early affected in barley seedlings treated with severe drought stress. Rather than focusing on individual genes, our strategy was pointed to the identification of groups of genes with similar expression patterns. As such, we identified and annotated almost 150 barley genes as crucial core-components of photosystems, electron transport components, and Calvin cycle enzymes. Moreover, we designated 17 possible regulatory interactions between photosynthesis-related genes and transcription factors in barley. Summarizing, our results provide a list of candidate genes for future genetic research and improvement of barley drought tolerance by targeting photosynthesis. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85076276262,

title = {Insights into barley root transcriptome under mild drought stress with an emphasis on gene expression regulatory mechanisms},

author = { A. Janiak and M. Kwaśniewski and M. Sowa and A. Kuczyńska and K. Mikołajczak and P. Ogrodowicz and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076276262&doi=10.3390%2fijms20246139&partnerID=40&md5=2b382a6fe5058d045506ac690a516d22},

doi = {10.3390/ijms20246139},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {24},

publisher = {MDPI AG},

abstract = {Root systems play a pivotal role in coupling with drought stress, which is accompanied with a substantial transcriptome rebuilding in the root tissues. Here, we present the results of global gene expression profiling of roots of two barley genotypes with contrasting abilities to cope with drought that were subjected to a mild level of the stress. We concentrate our analysis on gene expression regulation processes, which allowed the identification of 88 genes from 39 families involved in transcriptional regulation in roots upon mild drought. They include 13 genes encoding transcription factors (TFs) from AP2 family represented by ERFs, DREB, or B3 domain-containing TFs, eight WRKYs, six NACs, five of the HD-domain, MYB or MYB-related, bHLH and bZIP TFs. Also, the representatives of C3H, CPP, GRAS, LOB-domain, TCP, Tiffy, Tubby, and NF-Ys TFs, among others were found to be regulated by the mild drought in barley roots. We found that drought tolerance is accompanied with a lower number of gene expression changes than the amount observed in a susceptible genotype. The better drought acclimation may be related to the activation of transcription factors involved in the maintenance of primary root growth and in the epigenetic control of chromatin and DNA methylation. In addition, our analysis pointed to fives TFs from ERF, LOB, NAC, WRKY and bHLH families that may be important in the mild but not the severe drought response of barley roots. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {13},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85065722326,

title = {Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids},

author = { J. Majka and K. Bzdęga and A. Janiak and H. Ćwiek-Kupczyńska and P. Krajewski and T. Ksiązczyk and Z. Zwierzykowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065722326&doi=10.1186%2fs12864-019-5766-2&partnerID=40&md5=f2c182afbce1238217c89c8b71df8864},

doi = {10.1186/s12864-019-5766-2},

issn = {14712164},

year  = {2019},

date = {2019-01-01},

journal = {BMC Genomics},

volume = {20},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: Species of the Festuca and Lolium genera, as well as intergeneric Festuca × Lolium (Festulolium) hybrids, are valuable fodder and turf grasses for agricultural and amenity purposes worldwide. Festulolium hybrids can merge in their genomes agronomically important characteristics. However, in polyploid plants, especially in allopolyploids, the hybridization of divergent genomes could contribute to various abnormalities, such as variability in chromosome number, structural rearrangements, and/or disorders in inheritance patterns. Here we studied these issues in allotetraploid Festuca pratensis × Lolium perenne hybrids. Results: Cytogenetic procedures, including fluorescent in situ hybridization, genomic in situ hybridization, and molecular markers - inter-simple sequence repeats (ISSR) were exploited. This cytogenetic approach indicated the dynamics in the number and distribution of ribosomal RNA genes and structural rearrangements for both parental genomes (Festuca and Lolium) in hybrid karyotypes. The separate analysis of F. pratensis and L. perenne chromosomes in hybrid plants (F2-F3 generations of F. pratensis × L. perenne) revealed the asymmetrical level of rearrangements. Recognized structural changes were mainly located in the distal part of chromosome arms, and in chromosomes bearing ribosomal DNA, they were more frequently mapped in arms without this sequence. Based on the ISSR markers distribution, we found that the tetrasomic type of inheritance was characteristic for the majority of ISSR loci, but the disomic type was also observed. Nonetheless, no preference in the transmission of either Festuca or Lolium alleles to the following generations of allotetraploid F. pratensis × L. perenne hybrid was observed. Conclusion: Our study reports cytogenetic and molecular genotyping of the F. pratensis × L. perenne hybrid and its following F2-F3 progenies. The analysis of 137 allotetraploid F. pratensis × L. perenne hybrids revealed the higher level of recombination in chromosomes derived from F. pratensis genome. The results of ISSR markers indicated a mixed model of inheritance, which may be characteristic for these hybrids. © 2019 The Author(s).},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85054525335,

title = {Forward genetics approach reveals a mutation in bhlh transcription factor-encoding gene as the best candidate for the root hairless phenotype in barley},

author = { P. Gajewska and A. Janiak and M. Kwaśniewski and P. Kędziorski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054525335&doi=10.3389%2ffpls.2018.01229&partnerID=40&md5=371c060234e07274d47b748cccb6c210},

doi = {10.3389/fpls.2018.01229},

issn = {1664462X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Plant Science},

volume = {9},

publisher = {Frontiers Media S.A.},

abstract = {Root hairs are the part of root architecture contributing significantly to the root surface area. Their role is particularly substantial in maintaining plant growth under stress conditions, however, knowledge on mechanism of root hair differentiation is still limited for majority of crop species, including barley. Here, we report the results of a map-based identification of a candidate gene responsible for the lack of root epidermal cell differentiation, which results in the lack of root hairs in barley. The analysis was based on the root hairless barley mutant rhl1.b, obtained after chemical mutagenesis of spring cultivar ‘Karat’. The rhl1 gene was located in chromosome 7HS in our previous studies. Fine mapping allowed to narrow the interval encompassing rhl1 gene to 3.7 cM, which on physical barley map spans a region of 577 kb. Five high confidence genes are located within this region and their sequencing resulted in the identification of A>T mutation in one candidate, HORVU7Hr1G030250 (MLOC_38567), differing the mutant from its parent variety. The mutation, located in the 3′ splice-junction site, caused the retention of the last intron, 98 bp long, in mRNA of rhl1.b allele. This resulted in the frameshift, the synthesis of 71 abnormal amino acids and introduction of premature STOP codon in mRNA. The mutation was present in the recombinants from the mapping population (F2 rhl1.b × ‘Morex’) that lacked root hairs. The candidate gene encodes a bHLH transcription factor with LRL domain and may be involved in early stages of root hair cell development. We discuss the possible involvement of HORVU7Hr1G030250 in this process, as the best candidate responsible for early stages of rhizodermis differentiation in barley. © 2018 Gajewska, Janiak, Kwasniewski, Kędziorski and Szarejko.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85049182046,

title = {Prioritization of candidate genes in qtl regions for physiological and biochemical traits underlying drought response in barley (Hordeum vulgare L.)},

author = { K. Gudyś and J. Guzy-Wróbelska and A. Janiak and M.A. Dziurka and A. Ostrowska and K. Hura and B. Jurczyk and K. Żmuda and D. Grzybkowska and J. Śróbka and W. Urban and J. Biesaga-Kościelniak and M. Filek and J. Kościelniak and K. Mikołajczak and P. Ogrodowicz and K. Krystkowiak and A. Kuczyńska and P. Krajewski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049182046&doi=10.3389%2ffpls.2018.00769&partnerID=40&md5=ee4f20bff713e6791812c6e0fb2a84e8},

doi = {10.3389/fpls.2018.00769},

issn = {1664462X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Plant Science},

volume = {9},

publisher = {Frontiers Media S.A.},

abstract = {Drought is one of the most adverse abiotic factors limiting growth and productivity of them were genes encoding antioxidants, carboxylic acid biosynthesis enzymes, heat shock proteins, small auxin up-regulated RNAs, nitric oxide synthase, ATP sulfurylases, and proteins involved in regulation of flowering time. This global approach may be proposed for identification of new CGs that underlies QTLs responsible for complex traits. crops. Among them is barley, ranked fourth cereal worldwide in terms of harvested acreage and production. Plants have evolved various mechanisms to cope with water deficit at different biological levels, but there is an enormous challenge to decipher genes responsible for particular complex phenotypic traits, in order to develop drought tolerant crops. This work presents a comprehensive approach for elucidation of molecular mechanisms of drought tolerance in barley at the seedling stage of development. The study includes mapping of QTLs for physiological and biochemical traits associated with drought tolerance on a high-density function map, projection of QTL confidence intervals on barley physical map, and the retrievement of positional candidate genes (CGs), followed by their prioritization based on Gene Ontology (GO) enrichment analysis. A total of 64 QTLs for 25 physiological and biochemical traits that describe plant water status, photosynthetic efficiency, osmoprotectant and hormone content, as well as antioxidant activity, were positioned on a consensus map, constructed using RIL populations developed from the crosses between European and Syrian genotypes. The map contained a total of 875 SNP, SSR and CGs, spanning 941.86 cM with resolution of 1.1 cM. For the first time, QTLs for ethylene, glucose, sucrose, maltose, raffinose, a-tocopherol, g-tocotrienol content, and catalase activity, have been mapped in barley. Based on overlapping confidence intervals of QTLs, 11 hotspots were identified that enclosedmore than 60%ofmapped QTLs. Genetic and physicalmap integration allowed the identification of 1,101 positional CGs within the confidence intervals of drought response-specific QTLs. Prioritization resulted in the designation of 143 CGs, among them were genes encoding antioxidants, carboxylic acid biosynthesis enzymes, heat shock proteins, small auxin up-regulated RNAs, nitric oxide synthase, ATP sulfurylases, and proteins involved in regulation of flowering time. This global approach may be proposed for identification of new CGs that underlies QTLs responsible for complex traits. © 2018 Gudys, Guzy-Wrobelska, Janiak, Dziurka, Ostrowska, Hura, Jurczyk, Żmuda, Grzybkowska, Śróbka, Urban, Biesaga-Koscielniak, Filek, Koscielniak, Mikołajczak, Ogrodowicz, Krystkowiak, Kuczyńska, Krajewski and Szarejko.},

note = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85041351117,

title = {No time to waste: Transcriptome study reveals that drought tolerance in barley may be attributed to stressed-like expression patterns that exist before the occurrence of stress},

author = { A. Janiak and M. Kwaśniewski and M. Sowa and K. Gajek and K. Żmuda and J. Kościelniak and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041351117&doi=10.3389%2ffpls.2017.02212&partnerID=40&md5=29f8103607eab16eb24f63b99775273c},

doi = {10.3389/fpls.2017.02212},

issn = {1664462X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Plant Science},

volume = {8},

publisher = {Frontiers Media S.A.},

abstract = {Plant survival in adverse environmental conditions requires a substantial change in the metabolism, which is reflected by the extensive transcriptome rebuilding upon the occurrence of the stress. Therefore, transcriptomic studies offer an insight into the mechanisms of plant stress responses. Here, we present the results of global gene expression profiling of roots and leaves of two barley genotypes with contrasting ability to cope with drought stress. Our analysis suggests that drought tolerance results from a certain level of transcription of stress-influenced genes that is present even before the onset of drought. Genes that predispose the plant to better drought survival play a role in the regulatory network of gene expression, including several transcription factors, translation regulators and structural components of ribosomes. An important group of genes is involved in signaling mechanisms, with significant contribution of hormone signaling pathways and an interplay between ABA, auxin, ethylene and brassinosteroid homeostasis. Signal transduction in a drought tolerant genotype may be more efficient through the expression of genes required for environmental sensing that are active already during normal water availability and are related to actin filaments and LIMdomain proteins, which may function as osmotic biosensors. Better survival of drought may also be attributed to more effective processes of energy generation and more efficient chloroplasts biogenesis. Interestingly, our data suggest that several genes involved in a photosynthesis process are required for the establishment of effective drought response not only in leaves, but also in roots of barley. Thus, we propose a hypothesis that root plastids may turn into the anti-oxidative centers protecting root macromolecules from oxidative damage during drought stress. Specific genes and their potential role in building upa drought-tolerant barley phenotype is extensively discussedwith special emphasis on processes that take place in barley roots. When possible, the interconnections between particular factors are emphasized to drawa broader picture of the molecular mechanisms of drought tolerance in barley. © 2018 Janiak, Kwasniewski, Sowa, Gajek, Żmuda, Kościelniak.},

note = {35},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85021379974,

title = {Root hair mutations displace the barley rhizosphere microbiota},

author = { S. Robertson-Albertyn and R.A. Terrazas and K. Balbirnie and M. Blank and A. Janiak and I. Szarejko and B. Chmielewska and J. Karcz and J. Morris and P.E. Hedley and T.S. George and D. Bulgarelli},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021379974&doi=10.3389%2ffpls.2017.01094&partnerID=40&md5=4a26278da1771c0e5b88bfb6ea326305},

doi = {10.3389/fpls.2017.01094},

issn = {1664462X},

year  = {2017},

date = {2017-01-01},

journal = {Frontiers in Plant Science},

volume = {8},

publisher = {Frontiers Media S.A.},

abstract = {The rhizosphere, the thin layer of soil surrounding and influenced by plant roots, defines a distinct and selective microbial habitat compared to unplanted soil. The microbial communities inhabiting the rhizosphere, the rhizosphere microbiota, engage in interactions with their host plants which span from parasitism to mutualism. Therefore, the rhizosphere microbiota emerges as one of the determinants of yield potential in crops. Studies conducted with different plant species have unequivocally pointed to the host plant as a driver of the microbiota thriving at the root–soil interface. Thus far, the host genetic traits shaping the rhizosphere microbiota are not completely understood. As root hairs play a critical role in resource exchanges between plants and the rhizosphere, we hypothesized that they can act as a determinant of the microbiota thriving at the root–soil interface. To test this hypothesis, we took advantage of barley (Hordeum vulgare) mutant lines contrasting for their root hair characteristics. Plants were grown in two agricultural soils, differentiating in their organic matter contents, under controlled environmental conditions. At early stem elongation rhizosphere specimens were collected and subjected to high-resolution 16S rRNA gene profiling. Our data revealed that the barley rhizosphere microbiota is largely dominated by members of the phyla Bacteroidetes and Proteobacteria, regardless of the soil type and the root hair characteristics of the host plant. Conversely, ecological indices calculated using operational taxonomic units (OTUs) presence, abundance, and phylogeny revealed a significant impact of root hair mutations on the composition of the rhizosphere microbiota. In particular, our data indicate that mutant plants host a reduced-complexity community compared to wild-type genotypes and unplanted soil controls. Congruently, the host genotype explained up to 18% of the variation in ecological distances computed for the rhizosphere samples. Importantly, this effect is manifested in a soil-dependent manner. A closer inspection of the sequencing profiles revealed that the root hair-dependent diversification of the microbiota is supported by a taxonomically narrow group of bacteria, with a bias for members of the orders Actinomycetales, Burkholderiales, Rhizobiales, Sphingomonadales, and Xanthomonadales. Taken together, our results indicate that the presence and function of root hairs are a determinant of the bacterial community thriving in the rhizosphere and their perturbations can markedly impact on the recruitment of individual members of the microbiota. © 2017 Robertson-Albertyn, Alegria Terrazas, Balbirnie, Blank, Janiak, Szarejko, Chmielewska, Karcz, Morris, Hedley, George and Bulgarelli.},

note = {47},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84999759002,

title = {Phenotypic plasticity, epigenetic or genetic modifications in relation to the duration of Cd-exposure within a microevolution time range in the beet armyworm},

author = { M. Augustyniak and A. Płachetka-Bożek and A. Kafel and A. Babczyńska and M. Tarnawska and A. Janiak and A. Loba and M. Dziewięcka and J. Karpeta-Kaczmarek and A. Zawisza-Raszka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84999759002&doi=10.1371%2fjournal.pone.0167371&partnerID=40&md5=71387ac76e18d04e4111760b53546841},

doi = {10.1371/journal.pone.0167371},

issn = {19326203},

year  = {2016},

date = {2016-01-01},

journal = {PLoS ONE},

volume = {11},

number = {12},

publisher = {Public Library of Science},

abstract = {In the case of the pests inhabiting metal polluted or fields where the use of pesticides is common, a natural selection of resistant individuals can occur. This may pose serious problems for humans, agriculture, as well as the economies of many countries. In this study, the hypothesis that multigenerational (120 generations) exposure to cadmium of a beet armyworm population could be a selecting factor toward a more efficient DNA protection was verified. The hemocytes of individuals from two culture strains (control and Cd-exposed) were treated with H2O2 (a DNA-damaging agent) or PBS (reference). The level of DNA damage was assessed using the Comet assay immediately and 5, 15 and 30 min. after the treatment. The immediate result of the contact with H2O2 was that the level of DNA damage in the hemocytes of the insects from both strains increased significantly. However, in the cells of the Cd-exposed individuals, the level of DNA damage decreased over time, while in the cells from the control insects it remained at the same level with no evidence of repair. These results suggest that efficient defense mechanisms may exist in the cells of insects that have prolonged contact with cadmium. Some evolutionary and trade-off aspects of the phenomenon are discussed. In a wider context, comparing the results obtained in the laboratory with field studies may be beneficial for understanding basic mechanisms of the resistance of an organism. To summarize, the high potential for the repair of DNA damage that was observed in the insects from the cadmium strain may confirm the hypothesis that multigenerational exposure to that metal may possibly contribute to the selection of insects that have a wider tolerance to oxidative stress. However, our investigations of polymorphism using AFLP did not reveal differences between the two main insect strains. © 2016 Augustyniak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {23},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84991258049,

title = {A survey of genetic variation and genome evolution within the invasive Fallopia complex},

author = { K. Bzdęga and A. Janiak and T. Ksiązczyk and A. Lewandowska and M. Gancarek and E. Sliwinska and B. Tokarska-Guzik},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84991258049&doi=10.1371%2fjournal.pone.0161854&partnerID=40&md5=9b6fdbc28af38482cac72b2dab51becc},

doi = {10.1371/journal.pone.0161854},

issn = {19326203},

year  = {2016},

date = {2016-01-01},

journal = {PLoS ONE},

volume = {11},

number = {8},

publisher = {Public Library of Science},

abstract = {The knotweed taxa Fallopia japonica, F. sachalinensis and their interspecific hybrid F. × bohemica are some of the most aggressive invaders in Europe and North America and they are serious threats to native biodiversity. At the same time, they constitute a unique model system for the creation of hybrids and studies of the initiation of evolutionary processes. In the presented study, we focused on (i) examining genetic diversity in selected populations of three Fallopia taxa in the invaded (Poland) and native ranges (Japan), (ii) establishing genome size and ploidy levels and (iii) identifying ribosomal DNA (rDNA)-bearing chromosomes in all of the taxa from the invaded range. We found that the genetic diversity within particular taxa was generally low regardless of their geographical origin. A higher level of clonality was observed for the Polish populations compared to the Japanese populations. Our study suggests that the co-occurrence of F. sachalinensis together with the other two taxa in the same stand may be the source of the higher genetic variation within the F. × bohemica hybrid. Some shift towards the contribution of F. japonica alleles was also observed for selected F. × bohemica individuals, which indicates the possibility of producing more advanced generations of F. × bohemica hybrids. All of the F. sachalinensis individuals were hexaploid (2n = 6x = 66; 2C = 6.01 pg), while those of F. japonica were mostly octoploid (2n = 8x = 88; 2C = 8.87 pg) and all of the F. × bohemica plants except one were hexaploid (2n = 6x = 66; 2C = 6.46 pg). Within the chromosome complement of F. japonica, F. sachalinensis and F. × bohemica, the physical mapping of the rDNA loci provided markers for 16, 13 and 10 chromosomes, respectively. In F. × bohemica, a loss of some of rDNA loci was observed, which indicates the occurrence of genome changes in the hybrid. © 2016 Bzdega et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84960418233,

title = {Gene expression regulation in roots under drought},

author = { A. Janiak and M. Kwaśniewski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960418233&doi=10.1093%2fjxb%2ferv512&partnerID=40&md5=f0a05cb7766f83d02cb6ec95f5fd6a45},

doi = {10.1093/jxb/erv512},

issn = {00220957},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Experimental Botany},

volume = {67},

number = {4},

pages = {1003-1014},

publisher = {Oxford University Press},

abstract = {Stress signalling and regulatory networks controlling expression of target genes are the basis of plant response to drought. Roots are the first organs exposed to water deficiency in the soil and are the place of drought sensing. Signalling cascades transfer chemical signals toward the shoot and initiate molecular responses that lead to the biochemical and morphological changes that allow plants to be protected against water loss and to tolerate stress conditions. Here, we present an overview of signalling network and gene expression regulation pathways that are actively induced in roots under drought stress. In particular, the role of several transcription factor (TF) families, including DREB, AP2/ERF, NAC, bZIP, MYC, CAMTA, Alfin-like and Q-Type ZFP, in the regulation of root response to drought are highlighted. The information provided includes available data on mutual interactions between these TFs together with their regulation by plant hormones and other signalling molecules. The most significant downstream target genes and molecular processes that are controlled by the regulatory factors are given. These data are also coupled with information about the influence of the described regulatory networks on root traits and root development which may translate to enhanced drought tolerance. This is the first literature survey demonstrating the gene expression regulatory machinery that is induced by drought stress, presented from the perspective of roots. © 2015 The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.},

note = {93},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84960405354,

title = {Transcriptome analysis reveals the role of the root hairs as environmental sensors to maintain plant functions under water-deficiency conditions},

author = { M. Kwaśniewski and A. Daszkowska-Golec and A. Janiak and K. Chwiałkowska and U. Nowakowska and G. Sablok and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960405354&doi=10.1093%2fjxb%2ferv498&partnerID=40&md5=32765e7f173f0f3dbd3b50cefecd7d96},

doi = {10.1093/jxb/erv498},

issn = {00220957},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Experimental Botany},

volume = {67},

number = {4},

pages = {1079-1094},

publisher = {Oxford University Press},

abstract = {An important part of the root system is the root hairs, which play a role in mineral and water uptake. Here, we present an analysis of the transcriptomic response to water deficiency of the wild-Type (WT) barley cultivar 'Karat' and its root-hairless mutant rhl1.a. A comparison of the transcriptional changes induced by water stress resulted in the identification of genes whose expression was specifically affected in each genotype. At the onset of water stress, more genes were modulated by water shortage in the roots of the WT plants than in the roots of rhl1.a. The roots of the WT plants, but not of rhl1.a, specifically responded with the induction of genes that are related to the abscisic acid biosynthesis, stomatal closure, and cell wall biogenesis, thus indicating the specific activation of processes that are related to water-stress signalling and protection. On the other hand, the processes involved in the further response to abiotic stimuli, including hydrogen peroxide, heat, and high light intensity, were specifically up-regulated in the leaves of rhl1.a. An extended period of severe stress caused more drastic transcriptome changes in the roots and leaves of the rhl1.a mutant than in those of the WT. These results are in agreement with the much stronger damage to photosystem II in the rhl1.a mutant than in its parent cultivar after 10 d of water stress. Taking into account the putative stress sensing and signalling features of the root hair transcriptome, we discuss the role of root hairs as sensors of environmental conditions. © 2015 The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.},

note = {50},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84930040737,

title = {A study of the genetic variation of the aquatic fern Marsilea quadrifolia L. preserved in botanical collections in Poland and originated from natural populations in Europe},

author = { A. Janiak and K. Galej and J.B. Parusel and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930040737&doi=10.1016%2fj.flora.2014.08.011&partnerID=40&md5=8b21bef7d995a280f3ad583177aed701},

doi = {10.1016/j.flora.2014.08.011},

issn = {03672530},

year  = {2014},

date = {2014-01-01},

journal = {Flora: Morphology, Distribution, Functional Ecology of Plants},

volume = {209},

number = {11},

pages = {655-665},

publisher = {Elsevier GmbH},

abstract = {Aim of the present study was to evaluate the genetic diversity of selected European populations of Marsilea quadrifolia L. and to assess the applicability of those genetic resources of Marsilea quadrifolia L. that have been preserved in Polish botanical gardens, for the reintroduction of this species into its historical range in Poland. Three Polish populations that originated from botanical collections (Zabrze; Z˙arów and Puławy) and four natural populations (two from Slovakia (Slovakia I and Slovakia II); one from France and one from Germany) were analyzed using Amplified Fragment Length Polymorphism (AFLP) markers. A very low level of genetic variation was found both within and between the populations in the study, which likely resulted from a genetic bottleneck probably caused by human activities. Plants with the same AFLP fingerprint were found across several populations; however, singleton samples with a unique AFLP band pattern were also present within all of the populations. The presence of singletons led to relatively high values of Simpson's diversity index, which may suggest a considerable effect of mutations and some possibility of sexual reproduction as sources of the observed variation. The partitioning of molecular variance was calculated using hierarchical AMOVA, which showed that a negligible value of only 0.81% of the variation was explained by the category of population, i.e. plants originating from the botanical collections or from the natural habitats. This result indicates that M. quadrifolia populations from botanical collections resemble natural populations in terms of the level of their genetic variation and that the populations that were obtained from the Polish collections could be used for the successful reintroduction of this species into its historical range in Poland, and a similar situation may be given also in other areas of occurrence of this plant that is under threat throughout its area of occurrence in Europe. © 2014 Elsevier GmbH.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84927173271,

title = {Morphological, genetic and molecular characteristics of barley root hair mutants},

author = { B. Chmielewska and A. Janiak and J. Karcz and J. Guzy-Wróbelska and B.P. Forster and M. Nawrot and A. Rusek and P. Smyda and P. Kędziorski and M. Maluszynski and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927173271&doi=10.1007%2fs13353-014-0225-x&partnerID=40&md5=6c3835a594fbf5122995a378c846e7ad},

doi = {10.1007/s13353-014-0225-x},

issn = {12341983},

year  = {2014},

date = {2014-01-01},

journal = {Journal of Applied Genetics},

volume = {55},

number = {4},

pages = {433-447},

publisher = {Springer Verlag},

abstract = {Root hairs are tubular outgrowths of specialized epidermal cells called trichoblasts. They affect anchoring plants in soil, the uptake of water and nutrients and are the sites of the interaction between plants and microorganisms. Nineteen root hair mutants of barley representing different stages of root hair development were subjected to detailed morphological and genetic analyses. Each mutant was monogenic and recessive. An allelism test revealed that nine loci were responsible for the mutated root hair phenotypes in the collection and 1–4 mutated allelic forms were identified at each locus. Genetic relationships between the genes responsible for different stages of root hair formation were established. The linkage groups of four loci rhl1, rhp1, rhi1 and rhs1, which had previously been mapped on chromosomes 7H, 1H, 6H and 5H, respectively, were enriched with new markers that flank the genes at a distance of 0.16 cM to 4.6 cM. The chromosomal position of three new genes – two that are responsible for the development of short root hairs (rhs2 and rhs3) and the gene that controls an irregular root hair pattern (rhi2) – were mapped on chromosomes 6H, 2H and 1H, respectively. A comparative analysis of the agrobotanical parameters between some mutants and their respective parental lines showed that mutations in genes responsible for root hair development had no effect on the agrobotanical performance of plants that were grown under controlled conditions. The presented mutant collection is a valuable tool for further identification of genes controlling root hair development in barley. © The Author(s) 2014},

note = {14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84887033837,

title = {Vascular endothelial growth factor-A signaling in bone marrow-derived endothelial progenitor cells exposed to hypoxic stress},

author = { B.R. Hoffmann and J.R. Wagner and A.R. Prisco and A. Janiak and A.S. Greene},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887033837&doi=10.1152%2fphysiolgenomics.00070.2013&partnerID=40&md5=f3fef4085cce1a2a3e3bcb431ee29a63},

doi = {10.1152/physiolgenomics.00070.2013},

issn = {10948341},

year  = {2013},

date = {2013-01-01},

journal = {Physiological Genomics},

volume = {45},

number = {21},

pages = {1021-1034},

abstract = {Bone marrow-derived endothelial progenitor cells (BM-EPCs) are stimulated by vascular endothelial growth factor-A (VEGF-A) and other potent proangiogenic factors. During angiogenesis, an increase in VEGF-A expression stimulates BM-EPCs to enhance endothelial tube formation and contribute to an increase in microvessel density. Hypoxia is known to produce an enhanced angiogenic response and heightened levels of VEGF-A have been seen in oxygen deprived epithelial and endothelial cells, yet the pathways for VEGF-A signaling in BM-EPCs have not been described. This study explores the influence of hypoxia on VEGF-A signaling in rat BM-EPCs utilizing a novel proteomic strategy to directly identify interacting downstream components of the combined VEGF receptor(s) signaling pathways, gene expression analysis, and functional phenotyping. VEGF-A signaling network analysis following liquid chromatographic separation and tandem mass spectrometry revealed proteins related to inositol/cal-cium signaling, nitric oxide signaling, cell survival, cell migration, and inflammatory responses. Alterations in BM-EPC expression of common angiogenic genes and tube formation in response to VEGF-A during hypoxia were measured and combined with the proteomic analysis to enhance and support the signaling pathways detected. BM-EPC tube formation assays in response to VEGF-A exhibited little tube formation; however, a cell projection/migratory phenotype supported the signaling data. Additionally, a novel assay measuring BM-EPC incorporation into preformed endothelial cell tubes indicated a significant increase of incorporated BM-EPCs after pretreat-ment with VEGF-A during hypoxia. This study verifies known VEGF-A pathway components and reveals several unidentified mechanisms of VEGF-A signaling in BM-EPCs during hypoxia that may be important for migration to sites of vascular regeneration. © 2013 the American Physiological Society.},

note = {26},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84867766790,

title = {A comparative analysis of proteins that accumulate during the initial stage of root hair development in barley root hair mutants and their parent varieties},

author = { A. Janiak and S. Piórko and A. Matros and H.P. Mock and M. Kwaśniewski and K. Chwiałkowska and B. Chmielewska and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867766790&doi=10.1007%2fs13353-012-0105-1&partnerID=40&md5=565cdb3ffb698d5ea4b93df756518bca},

doi = {10.1007/s13353-012-0105-1},

issn = {12341983},

year  = {2012},

date = {2012-01-01},

journal = {Journal of Applied Genetics},

volume = {53},

number = {4},

pages = {363-376},

abstract = {The mechanisms of root hair formation have been studied extensively in Arabidopsis but knowledge about these processes in monocot species is still limited, especially in relation to the proteome level. The aim of this study was to identify the proteins that are involved in the initiation and the early stage of root hair tip growth in barley using two-dimensional (2D) electrophoresis and mass spectrometry. A comparison of proteins that accumulate differentially in two root hair mutants and their respective parent varieties resulted in the identification of 13 proteins that take part in several processes related to the root hair morphogenesis, such as the control of vesicular trafficking, ROS signalling and homeostasis, signal transduction by phospholipids metabolism and ATP synthesis. Among the identified proteins, two ATP synthases, two ABC transporters, a small GTPase from the SAR1 family, a PDI-like protein, a monodehydroascorbate reductase, a C2 domain-containing protein and a Wali7 domain-containing protein were found. This study is the first report on the proteins identified in the initial stage of root hair formation in barley and gives new insights into the mechanisms of root hair morphogenesis in a monocot species. © 2012 The Author(s).},

note = {17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84865992818,

title = {Unexpected genetic diversity of Fallopia japonica from Central Europe revealed after AFLP analysis},

author = { K. Bzdęga and A. Janiak and S. Tarłowska and M.M. Kurowska and B. Tokarska-Guzik and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865992818&doi=10.1016%2fj.flora.2012.05.002&partnerID=40&md5=6fae375b33865dfffbbe0280848f867c},

doi = {10.1016/j.flora.2012.05.002},

issn = {03672530},

year  = {2012},

date = {2012-01-01},

journal = {Flora: Morphology, Distribution, Functional Ecology of Plants},

volume = {207},

number = {9},

pages = {636-645},

abstract = {Recently much attention has been paid to genetic aspects of invasive success in Japanese knotweed s.l. One hypothesis to explain the invasive spread of these species is a multiple introduction, which leads to a higher level of genetic diversity in the invaded range. Fallopia japonica is considered to be genetically uniform in Europe, introduced as a single female clone. However, there is some evidence suggesting that invasion history and dynamics differ between Western and Central-Eastern Europe. We used AFLP markers to characterize genetic diversity of three Fallopia taxa that occur in Poland: F. japonica, F. sachalinensis and their hybrid Fallopia×. bohemica, growing in so-called 'homogeneous' populations, consisting of one taxon and 'heterogeneous' populations, composed of the three taxa cohabiting together. No polymorphism, resp. an insignificantly low variability was observed in the 'homogeneous' populations. In the 'heterogeneous' stands polymorphism was detected within each taxa, with one exception that concerns individuals of F. sachalinensis from a riparian habitat. The highest level of polymorphism was found among individuals of F.×. bohemica. The most striking result of our study is the observation of polymorphism between individuals of F. japonica. The AFLP data also showed that F.×. bohemica is most diverse when occurring in a heterogeneous configuration with F. japonica and F. sachalinensis in the same habitat. Our results are the first evidence of genetic diversity in F. japonica populations in Central Europe and can implicate the possibility of its multiple introduction in this region or the existence of sexual reproduction of this species. © 2012 .},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77954533927,

title = {Global analysis of the root hair morphogenesis transcriptome reveals new candidate genes involved in root hair formation in barley},

author = { M. Kwaśniewski and A. Janiak and B. Mueller-Roeber and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954533927&doi=10.1016%2fj.jplph.2010.02.009&partnerID=40&md5=62dd9eadf3df2a4104cc214706a23f42},

doi = {10.1016/j.jplph.2010.02.009},

issn = {01761617},

year  = {2010},

date = {2010-01-01},

journal = {Journal of Plant Physiology},

volume = {167},

number = {13},

pages = {1076-1083},

abstract = {Root hairs are long tubular outgrowths of specialized root epidermal cells that play an important role in plant nutrition and water uptake. They are also an important model in studies of higher plant cell differentiation. In contrast to the model dicot Arabidopsis thaliana, currently very little is known about the genetic and molecular basis of root hair formation in monocots, including major cereals. To elucidate candidate genes controlling this developmental process in barley, we took advantage of the recently established Affymetrix GeneChip Barley1 Genome Array to carry out global transcriptome analyses of hairless and root hair primordia-forming roots of two barely mutant lines. Expression profiling of the root-hairless mutant rhl1.a and its wild type parent variety 'Karat' revealed 10 genes potentially involved in the early step of root hair formation in barley. Differential expression of all identified genes was confirmed by quantitative reverse transcription-polymerase chain reaction. The genes identified encode proteins associated with the cell wall and membranes, including one gene for xyloglucan endotransglycosylase, three for peroxidase enzymes and five for arabinogalactan protein, extensin, leucine-rich-repeat protein, phosphatidylinositol phosphatidylcholine transfer protein and a RhoGTPase GDP dissociation inhibitor, respectively. The molecular function of one gene is unknown at present. The expression levels of these genes were strongly reduced in roots of the root-hairless mutant rhl1.a compared to the parent variety, while expression of all 10 genes was similar in another mutant, i.e. rhp1.b, that has lost its ability to develop full root hairs but still forms hairs blocked at the primordium stage, and its wild type relative. This clearly indicates that the new genes identified are involved in the initiation of root hair morphogenesis in barley. © 2010 Elsevier GmbH.},

note = {33},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-44849101771,

title = {Application of degenerate oligonucleotide primed PCR (DOP-PCR) for SNP discovery in soybean},

author = { A. Janiak and M.Y. Kim and K. Van and S.H. Lee},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-44849101771&doi=10.1007%2fs10681-007-9599-8&partnerID=40&md5=92af4540b3c9e1c960e3748dfd49f430},

doi = {10.1007/s10681-007-9599-8},

issn = {00142336},

year  = {2008},

date = {2008-01-01},

journal = {Euphytica},

volume = {162},

number = {2},

pages = {249-256},

abstract = {As the majority of methods for single nucleotide polymorphism (SNP) identification are highly cost-prohibitive, it is necessary to develop new strategies that are more suitable for small and medium-scale laboratories. In this paper, we investigate the potential of degenerate oligonucleotide primed PCR (DOP-PCR) for SNP discovery in soybean. PCR fragments were amplified from two soybean cultivars, 'Pureunkong' and 'Jinpumkong 2,' shotgun cloned and sequenced. The sequences of both cultivars were then assembled and examined for occurrence of SNPs. The effectiveness of SNP discovery was much lower than expected. Over 1,300 analyzed sequences were grouped in 144 contigs, but only 51 putative SNP sites were found in 18 of these contigs. About 50% of the contigs contained identical sequences and in more than one-third (35.4%), putative paralogous fragments were assembled. Subsequent validation of SNPs allowed the confirmation of only eight SNP sites. The failure to validate the remaining SNPs was mostly due to amplification of duplicated or multiplicated genomic regions. A relatively high proportion of chloroplast and mitochondrial DNA sequences was another limitation of effective SNP detection. Although the DOP-PCR technique was not efficient enough for SNP discovery because of the degree of soybean genome complication, the relatively large number of paralogous sequences in our data collection can be used for further detailed analysis of the genome structure of this species. © 2007 Springer Science+Business Media B.V.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-42949138922,

title = {The protective role of selenium in rape seedlings subjected to cadmium stress},

author = { M. Filek and R. Keskinen and H. Hartikainen and I. Szarejko and A. Janiak and Z. Miszalski and A. Golda},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-42949138922&doi=10.1016%2fj.jplph.2007.06.006&partnerID=40&md5=ec4848f0ba96e10a67d3b3ef804b741e},

doi = {10.1016/j.jplph.2007.06.006},

issn = {01761617},

year  = {2008},

date = {2008-01-01},

journal = {Journal of Plant Physiology},

volume = {165},

number = {8},

pages = {833-844},

publisher = {Elsevier GmbH},

abstract = {The effect of selenium (Se) on rape (Brassica napus) seedlings subjected to cadmium (Cd) stress was studied in vitro by investigating plant growth and changes in fatty acid composition, activity of antioxidative enzymes and DNA methylation pattern. Physiological experiments were carried out on seedlings cultured for 2 weeks on Murashige-Scoog (MS) media with Cd concentrations of 0, 400 and 600 μM, and on corresponding media supplied with Se (2 μM). Exposure to increasing Cd concentrations reduced the fresh weight of the upper part (hypocotyls+cotyledons) of the seedlings more strongly than that of the root system, which was accompanied by higher Cd accumulation in these tissues. In the upper part, Cd exposure led to significant changes in the biochemical parameters: fatty acid unsaturation of plasmalemma decreased, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPOX) diminished and that of ascorbate peroxidase (APX) increased. In contrast, the roots showed an increase in fatty acid unsaturation and in the activity of antioxidative enzymes. In both parts of rape seedlings H2O2 level and lipid peroxidation increased. Se addition to medium considerably reversed the Cd-induced decrease in fresh mass as well as the changes in lipid unsaturation and peroxidation. Se applied separately or in combination with Cd did not significantly affect the activity of antioxidative enzymes in the roots, but diminished it in the upper part. Moreover, the presence of Se in medium prevented changes in the DNA methylation pattern triggered in rape seedlings by high Cd concentrations. Two possible mechanisms for the action of Se were considered: (1) removal of Cd from metabolically active cellular sites, and (2) reduction of oxygen radicals. © 2007 Elsevier GmbH. All rights reserved.},

note = {212},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34548300008,

title = {Molecular mapping of genes involved in root hair formation in barley},

author = { A. Janiak and I. Szarejko},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34548300008&doi=10.1007%2fs10681-007-9399-1&partnerID=40&md5=20ee9c6f6fe09f21e0da1e15abac390d},

doi = {10.1007/s10681-007-9399-1},

issn = {00142336},

year  = {2007},

date = {2007-01-01},

journal = {Euphytica},

volume = {157},

number = {1-2},

pages = {95-111},

abstract = {In the presented study, the existing AFLP and SSR maps of barley were used to find chromosomal position of four genes controlling different stages of root hair development. Four barley mutants were used in the analysis: the root hairless mutant rhl1.b, mutant rhp1.b with root hair development blocked at the initial bulge formation, mutant rhi1.a with irregular pattern of sparsely located root hairs and mutant rhs1.a with very short root hairs. Each mutant was crossed with parents of 'Steptoe'/'Morex' mapping population and F2 progenies of crosses: mutant × 'Steptoe' and mutant × 'Morex' were analyzed for segregation of root hair phenotype and polymorphic AFLP and SSR markers. It was possible to map all the analyzed genes on barley chromosomes: rhl1 gene on the short arm of chromosome 7H, rhp1 gene on chromosome 1H, rhs1 locus in the pericentromeric region of chromosome 5H and rhi1 gene on the long arm of chromosome 6H. Subsequently, the Bulk Segregant Analysis and AFLP technique were used for saturation of the identified regions with new markers. The joint maps were constructed using as common points the SSR markers located in the target regions. Linkage maps of the regions around the four genes involved in the root hair formation in barley were composed of 8-11 markers and spanned over 16.1-49.0 cM. The distances between localized genes and the closest markers ranged from 1.0 to 3.8 cM. The identified chromosomal locations of genes can be used for their fine mapping and future map-based cloning. © 2007 Springer Science+Business Media B.V.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33746031805,

title = {Does DNA methylation pattern mark generative development in winter rape?},

author = { M. Filek and A. Janiak and I. Szarejko and J. Grabczyńska and I. Macháčková and J. Krekule},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33746031805&doi=10.1515%2fznc-2006-5-615&partnerID=40&md5=1c7564715c40614ce8264fd84de0270d},

doi = {10.1515/znc-2006-5-615},

issn = {09395075},

year  = {2006},

date = {2006-01-01},

journal = {Zeitschrift fur Naturforschung - Section C Journal of Biosciences},

volume = {61},

number = {5-6},

pages = {387-396},

publisher = {Verlag der Zeitschrift fur Naturforschung},

abstract = {In this paper we report on changes in DNA methylation pattern in rape apices and leaves during transition from vegetative to reproductive stage due to grafting and/or vernalization. Grafted plants of winter rape (Brassica napus L.; var. "Górczański") (stock from vernalized; scion from non-vernalized plants) were used together with vernalized non-grafted plants. In addition, methylation status was determined also in spring rape (var. "Młochowski") grown under normal and low temperature. The methylation-sensitive amplification polymorphism (MSAP) method with EcoRI/MspI and EcoRH/HpaII restriction enzymes was employed. The majority (ca. 68%) of analyzed loci (566 in winter and 551 in spring rape) were monomorphic, i.e. did not undergo methylation. Both cultivars showed a similar degree of methylation. 188 loci in winter and 176 in spring cultivars expressed changes in the methylation pattern. All differentially amplified fragments resulted from either full methylation of an internal cytosine or from hemi-methylation of an external cytosine. A pair-wise comparison showed that a similar number of loci underwent development-related methylation changes in apices of the winter and spring rape. The majority (80%) of changes were demethylation events in generative (vernalized) apices of the winter cultivar. However, an increased number of demethylated loci was detected in vernalized apices in comparison with generative, non-vernalized ones. In apices of vegetative and generative grafted plants the same number of demethylation events was observed. Overall, 10 MSAP loci were detected that expressed methylation changes in vernalized apices only; among them 7 loci underwent demethylation after vernalization and remained methylated in both vegetative and generative non-vernalized stage. Only 1 locus was demethylated in generative non-vernalized apices. Thus, most of demethylation events can be ascribed to vernalization and not to the generative stage. In leaves of winter rape methylation and demethylation events occurred with similar frequency, while in the spring cultivar more demethylation events were detected. The results show that during vernalization and transition to the generative stage different sets of genes are activated. © 2006 Verlag der Zeitschrift für Naturforschung.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}