2017
Milewska-Hendel, A.; Baczewska, A. H.; Sala, K.; Dmuchowski, W.; Bragoszewska, P.; Gozdowski, D.; Jozwiak, A.; Chojnacki, T.; Swiezewska, E.; Kurczyńska, E. U.
In: PLoS ONE, vol. 12, no. 2, 2017, ISSN: 19326203, (20).
@article{2-s2.0-85013892983,
title = {Quantitative and qualitative characteristics of cell wall components and prenyl lipids in the leaves of Tilia x euchlora trees growing under salt stress},
author = { A. Milewska-Hendel and A.H. Baczewska and K. Sala and W. Dmuchowski and P. Bragoszewska and D. Gozdowski and A. Jozwiak and T. Chojnacki and E. Swiezewska and E.U. Kurczyńska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013892983&doi=10.1371%2fjournal.pone.0172682&partnerID=40&md5=9273a1f5b327a5d5316ca4927ba2751f},
doi = {10.1371/journal.pone.0172682},
issn = {19326203},
year = {2017},
date = {2017-01-01},
journal = {PLoS ONE},
volume = {12},
number = {2},
publisher = {Public Library of Science},
abstract = {The study was focused on assessing the presence of arabinogalactan proteins (AGPs) and pectins within the cell walls as well as prenyl lipids, sodium and chlorine content in leaves of Tilia x euchlora trees. The leaves that were analyzed were collected from trees with and without signs of damage that were all growing in the same salt stress conditions. The reason for undertaking these investigations was the observations over many years that indicated that there are trees that present a healthy appearance and trees that have visible symptoms of decay in the same habitat. Leaf samples were collected from trees growing in the median strip between roadways that have been intensively salted during the winter season for many years. The sodium content was determined using atomic spectrophotometry, chloride using potentiometric titration and poly-isoprenoids using HPLC/UV. AGPs and pectins were determined using immunohistochemistry methods. The immunohistochemical analysis showed that rhamnogalacturonans I (RG-I) and homogalacturonans were differentially distributed in leaves from healthy trees in contrast to leaves from injured trees. In the case of AGPs, the most visible difference was the presence of the JIM16 epitope. Chemical analyses of sodium and chloride showed that in the leaves from injured trees, the level of these ions was higher than in the leaves from healthy trees. Based on chromatographic analysis, four polyisoprenoid alcohols were identified in the leaves of T. x euchlora. The levels of these lipids were higher in the leaves from healthy trees. The results suggest that the differences that were detected in the apoplast and symplasm may be part of the defensive strategy of T. x euchlora trees to salt stress, which rely on changes in the chemical composition of the cell wall with respect to the pectic and AGP epitopes and an increased synthesis of prenyl lipids. © 2017 Milewska-Hendel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},
note = {20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The study was focused on assessing the presence of arabinogalactan proteins (AGPs) and pectins within the cell walls as well as prenyl lipids, sodium and chlorine content in leaves of Tilia x euchlora trees. The leaves that were analyzed were collected from trees with and without signs of damage that were all growing in the same salt stress conditions. The reason for undertaking these investigations was the observations over many years that indicated that there are trees that present a healthy appearance and trees that have visible symptoms of decay in the same habitat. Leaf samples were collected from trees growing in the median strip between roadways that have been intensively salted during the winter season for many years. The sodium content was determined using atomic spectrophotometry, chloride using potentiometric titration and poly-isoprenoids using HPLC/UV. AGPs and pectins were determined using immunohistochemistry methods. The immunohistochemical analysis showed that rhamnogalacturonans I (RG-I) and homogalacturonans were differentially distributed in leaves from healthy trees in contrast to leaves from injured trees. In the case of AGPs, the most visible difference was the presence of the JIM16 epitope. Chemical analyses of sodium and chloride showed that in the leaves from injured trees, the level of these ions was higher than in the leaves from healthy trees. Based on chromatographic analysis, four polyisoprenoid alcohols were identified in the leaves of T. x euchlora. The levels of these lipids were higher in the leaves from healthy trees. The results suggest that the differences that were detected in the apoplast and symplasm may be part of the defensive strategy of T. x euchlora trees to salt stress, which rely on changes in the chemical composition of the cell wall with respect to the pectic and AGP epitopes and an increased synthesis of prenyl lipids. © 2017 Milewska-Hendel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
2002
Dobrowolska, I.; Kurczyńska, E. U.; Dmuchowski, W.
Anatomy of abscission zone of Betula pendula (Roth.) leaves from trees growing under different levels of pollution Journal Article
In: Cuadernos de Medicina Forense, no. 28, pp. 13-19, 2002, ISSN: 11357606, (2).
@article{2-s2.0-52449119574,
title = {Anatomy of abscission zone of Betula pendula (Roth.) leaves from trees growing under different levels of pollution},
author = { I. Dobrowolska and E.U. Kurczyńska and W. Dmuchowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-52449119574&partnerID=40&md5=fe391ead6ea674355d76f00620695645},
issn = {11357606},
year = {2002},
date = {2002-01-01},
journal = {Cuadernos de Medicina Forense},
number = {28},
pages = {13-19},
abstract = {A study was carried out on the leaf abscission zone from birch trees growing on polluted sites (two) and a non-polluted site (one). Samples for anatomical investigation were collected from six trees on each site, during three succeeding vegetation seasons. It was observed that in trees growing at the polluted sites: 1) maturation of the abscission zone was faster, 2) the protective layer was thinner and 3) the formation of leaf scar periderm was delayed in comparison with trees from the non-polluted site. The results obtained suggest that environmental pollution influences the formation of the abscission zone and the protection of the leaf scar.},
note = {2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A study was carried out on the leaf abscission zone from birch trees growing on polluted sites (two) and a non-polluted site (one). Samples for anatomical investigation were collected from six trees on each site, during three succeeding vegetation seasons. It was observed that in trees growing at the polluted sites: 1) maturation of the abscission zone was faster, 2) the protective layer was thinner and 3) the formation of leaf scar periderm was delayed in comparison with trees from the non-polluted site. The results obtained suggest that environmental pollution influences the formation of the abscission zone and the protection of the leaf scar.
2001
Dobrowolska, I.; Kurczyńska, E. U.; Dmuchowski, W.
Anatomy of abscission zone of betula pendula (Roth.) leaves from trees growing under different levels of pollution Journal Article
In: Dendrobiology, vol. 46, pp. 13-19, 2001, ISSN: 16411307, (2).
@article{2-s2.0-52549113262,
title = {Anatomy of abscission zone of betula pendula (Roth.) leaves from trees growing under different levels of pollution},
author = { I. Dobrowolska and E.U. Kurczyńska and W. Dmuchowski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-52549113262&partnerID=40&md5=ea13dfeb3b8ceba425bf0c7c5fadb9aa},
issn = {16411307},
year = {2001},
date = {2001-01-01},
journal = {Dendrobiology},
volume = {46},
pages = {13-19},
publisher = {Polska Akademia Nauk},
abstract = {A study was carried out on the leaf abscission zone from birch trees growing on polluted sites (two) and a non-polluted site (one). Samples for anatomical investigation were collected from six trees on each site, during three succeeding vegetation seasons. It was observed that in trees growing at the polluted sites: 1) maturation of the abscission zone was faster, 2) the protective layer was thinner and 3) the formation of leaf scar periderm was delayed in comparison with trees from the non-polluted site. The results obtained suggest that environmental pollution influences the formation of the abscission zone and the protection of the leaf scar.},
note = {2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A study was carried out on the leaf abscission zone from birch trees growing on polluted sites (two) and a non-polluted site (one). Samples for anatomical investigation were collected from six trees on each site, during three succeeding vegetation seasons. It was observed that in trees growing at the polluted sites: 1) maturation of the abscission zone was faster, 2) the protective layer was thinner and 3) the formation of leaf scar periderm was delayed in comparison with trees from the non-polluted site. The results obtained suggest that environmental pollution influences the formation of the abscission zone and the protection of the leaf scar.
1997
Kurczyńska, E. U.; Dmuchowski, W.; Włoch, W.; Bytnerowicz, A.
The influence of air pollutants on needles and stems of Scots pine (Pinus sylvestris L.) trees Journal Article
In: Environmental Pollution, vol. 98, no. 3, pp. 325-334, 1997, ISSN: 02697491, (66).
@article{2-s2.0-0345451538,
title = {The influence of air pollutants on needles and stems of Scots pine (Pinus sylvestris L.) trees},
author = { E.U. Kurczyńska and W. Dmuchowski and W. Włoch and A. Bytnerowicz},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0345451538&doi=10.1016%2fS0269-7491%2897%2900141-3&partnerID=40&md5=879e3c66918167da8c65164c9b78e824},
doi = {10.1016/S0269-7491(97)00141-3},
issn = {02697491},
year = {1997},
date = {1997-01-01},
journal = {Environmental Pollution},
volume = {98},
number = {3},
pages = {325-334},
abstract = {The influence of air pollution on the chemical composition of needles and stem anatomy (cambium, phloem, and xylem) of Scots pines (Pinus sylvestris L.) was examined in polluted and control forest sites near Warsaw, Poland. Foliar chemical composition was determined as an indicator of contamination of the trees examined anatomically. Anatomical analysis was carried out on samples including phloem, cambium, and current-year xylem collected at the breast height and from the tree top of the leader during one growing season. At the control site in current-year foliage, concentrations were N 1.53%, P 0.18%, K 0.5%, Ca 0.17%, Mg 910 ppm, Na 45 ppm, and S 0.145%, while at the polluted site concentrations were N 1.73%, P 0.18%, K 0.66%, Ca 0.35%, Mg 1509 ppm, Na 105 ppm and S 0.201%. Concentrations of heavy metals were significantly higher at the polluted site; only Mn was found in lower concentration. In trees from the polluted site, anatomical analysis revealed a decreased number of cambial cells at the beginning of growing season, phloem cells (at the tree top average number in a radial file was 21 and 14 in control and polluted sites, respectively), and formation of false rings with tracheids, similar to those in compression wood. Anatomical changes observed in trees from polluted sites are most likely caused by toxic effects of pollutants.},
note = {66},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The influence of air pollution on the chemical composition of needles and stem anatomy (cambium, phloem, and xylem) of Scots pines (Pinus sylvestris L.) was examined in polluted and control forest sites near Warsaw, Poland. Foliar chemical composition was determined as an indicator of contamination of the trees examined anatomically. Anatomical analysis was carried out on samples including phloem, cambium, and current-year xylem collected at the breast height and from the tree top of the leader during one growing season. At the control site in current-year foliage, concentrations were N 1.53%, P 0.18%, K 0.5%, Ca 0.17%, Mg 910 ppm, Na 45 ppm, and S 0.145%, while at the polluted site concentrations were N 1.73%, P 0.18%, K 0.66%, Ca 0.35%, Mg 1509 ppm, Na 105 ppm and S 0.201%. Concentrations of heavy metals were significantly higher at the polluted site; only Mn was found in lower concentration. In trees from the polluted site, anatomical analysis revealed a decreased number of cambial cells at the beginning of growing season, phloem cells (at the tree top average number in a radial file was 21 and 14 in control and polluted sites, respectively), and formation of false rings with tracheids, similar to those in compression wood. Anatomical changes observed in trees from polluted sites are most likely caused by toxic effects of pollutants.