2018
Skrzypek, E.; Warzecha, T.; Noga, A.; Warchoł, M.; Czyczyło-Mysza, I.; Dziurka, K.; Marcińska, I.; Kapłoniak, K.; Sutkowska, A.; Nita, Z.; Werwińska, K.; Idziak-Helmcke, D.; Rojek-Jelonek, M.; Hosiawa-Barańska, M.
Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.) Journal Article
In: PeerJ, vol. 2018, no. 6, 2018, ISSN: 21678359, (4).
@article{2-s2.0-85049247123,
title = {Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.)},
author = { E. Skrzypek and T. Warzecha and A. Noga and M. Warchoł and I. Czyczyło-Mysza and K. Dziurka and I. Marcińska and K. Kapłoniak and A. Sutkowska and Z. Nita and K. Werwińska and D. Idziak-Helmcke and M. Rojek-Jelonek and M. Hosiawa-Barańska},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049247123&doi=10.7717%2fpeerj.5107&partnerID=40&md5=0325cd81ea9a5e47f329ef405e3a02ce},
doi = {10.7717/peerj.5107},
issn = {21678359},
year = {2018},
date = {2018-01-01},
journal = {PeerJ},
volume = {2018},
number = {6},
publisher = {PeerJ Inc.},
abstract = {Background. The oat×maize addition (OMA) lines are used for mapping of the maize genome, the studies of centromere-specific histone (CENH3), gene expression, meiotic chromosome behavior and also for introducing maize C4 photosynthetic system to oat. The aim of our study was the identification and molecular-cytogenetic characterization of oat × maize hybrids. Methods. Oat DH lines and oat × maize hybrids were obtained using the wide crossing of Avena sativa L. with Zea mays L. The plants identified as having a Grande- 1 retrotransposon fragment, which produced seeds, were used for genomic in situ hybridization (GISH). Results. A total of 138 oat lines obtained by crossing of 2,314 oat plants from 80 genotypes with maize cv. Waza were tested for the presence of maize chromosomes. The presence of maize chromatin was indicated in 66 lines by amplification of the PCR product (500 bp) generated using primers specific for the maize retrotransposon Grande-1. Genomic in situ hybridization (GISH) detected whole maize chromosomes in eight lines (40%). All of the analyzed plants possessed full complement of oat chromosomes. The number of maize chromosomes differed between the OMA lines. Four OMA lines possessed two maize chromosomes similar in size, three OMA-one maize chromosome, and one OMA-four maize chromosomes. In most of the lines, the detected chromosomes were labeled uniformly. The presence of six 45S rDNA loci was detected in oat chromosomes, but none of the added maize chromosomes in any of the lines carried 45S rDNA locus. Twenty of the analyzed lines did not possess whole maize chromosomes, but the introgression of maize chromatin in the oat chromosomes. Five of 66 hybrids were shorter in height, grassy type without panicles. Twenty-seven OMA lines were fertile and produced seeds ranging in number from 1-102 (in total 613). Sixty-three fertile DH lines, out of 72 which did not have an addition of maize chromosomes or chromatin, produced seeds in the range of 1-343 (in total 3;758). Obtained DH and OMA lines were fertile and produced seeds. Discussion. In wide hybridization of oat with maize, the complete or incomplete chromosomes elimination of maize occur. Hybrids of oat and maize had a complete set of oat chromosomes without maize chromosomes, and a complete set of oat chromosomes with one to four retained maize chromosomes. © 2018 Skrzypek et al.},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Background. The oat×maize addition (OMA) lines are used for mapping of the maize genome, the studies of centromere-specific histone (CENH3), gene expression, meiotic chromosome behavior and also for introducing maize C4 photosynthetic system to oat. The aim of our study was the identification and molecular-cytogenetic characterization of oat × maize hybrids. Methods. Oat DH lines and oat × maize hybrids were obtained using the wide crossing of Avena sativa L. with Zea mays L. The plants identified as having a Grande- 1 retrotransposon fragment, which produced seeds, were used for genomic in situ hybridization (GISH). Results. A total of 138 oat lines obtained by crossing of 2,314 oat plants from 80 genotypes with maize cv. Waza were tested for the presence of maize chromosomes. The presence of maize chromatin was indicated in 66 lines by amplification of the PCR product (500 bp) generated using primers specific for the maize retrotransposon Grande-1. Genomic in situ hybridization (GISH) detected whole maize chromosomes in eight lines (40%). All of the analyzed plants possessed full complement of oat chromosomes. The number of maize chromosomes differed between the OMA lines. Four OMA lines possessed two maize chromosomes similar in size, three OMA-one maize chromosome, and one OMA-four maize chromosomes. In most of the lines, the detected chromosomes were labeled uniformly. The presence of six 45S rDNA loci was detected in oat chromosomes, but none of the added maize chromosomes in any of the lines carried 45S rDNA locus. Twenty of the analyzed lines did not possess whole maize chromosomes, but the introgression of maize chromatin in the oat chromosomes. Five of 66 hybrids were shorter in height, grassy type without panicles. Twenty-seven OMA lines were fertile and produced seeds ranging in number from 1-102 (in total 613). Sixty-three fertile DH lines, out of 72 which did not have an addition of maize chromosomes or chromatin, produced seeds in the range of 1-343 (in total 3;758). Obtained DH and OMA lines were fertile and produced seeds. Discussion. In wide hybridization of oat with maize, the complete or incomplete chromosomes elimination of maize occur. Hybrids of oat and maize had a complete set of oat chromosomes without maize chromosomes, and a complete set of oat chromosomes with one to four retained maize chromosomes. © 2018 Skrzypek et al.
2015
Sutkowska, A.; Pasierbiński, A.; Bąba, W.; Warzecha, T.; Mitka, J.
Additivity of ISSR markers in natural hybrids of related forest species Bromus benekenii and B. Ramosus (Poaceae) Journal Article
In: Acta Biologica Cracoviensia Series Botanica, vol. 57, no. 1, pp. 82-94, 2015, ISSN: 00015296, (6).
@article{2-s2.0-84944878600,
title = {Additivity of ISSR markers in natural hybrids of related forest species Bromus benekenii and B. Ramosus (Poaceae)},
author = { A. Sutkowska and A. Pasierbiński and W. Bąba and T. Warzecha and J. Mitka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84944878600&doi=10.1515%2fabcsb-2015-0015&partnerID=40&md5=81475ac731b5b56ffe3cef21fdedf44c},
doi = {10.1515/abcsb-2015-0015},
issn = {00015296},
year = {2015},
date = {2015-01-01},
journal = {Acta Biologica Cracoviensia Series Botanica},
volume = {57},
number = {1},
pages = {82-94},
publisher = {Polish Academy of Sciences Publishing House},
abstract = {The co-occurrence of hybrids and parental species in similar ecological niches poses a question on the role of traits additivity and overdispersion (emergence of new traits) in microevolutionary processes. We analysed genetic polymorphism of Bromus benekenii, B. ramosus and the spontaneous hybrid B. benekenii × B. ramosus in sympatric and allopatric parts of the species distribution in Europe, based on non-coding regions of the taxon genomes (ISSR genetic fingerprinting). We tested 68 individuals in 7 populations, including a hybrid population in N France. Altogether 233 polymorphic ISSR bands (loci) were obtained. We found that the parent species were genetically distinct and the hybrids had an additive pattern of ISSR bands found in the putative parental species (NMDS; STRUCTURE); however, there was evidence of introgression towards B. ramosus (NEWHYBRIDS; UPGMA classifications; Nei's D genetic distance). Bromus benekenii had 72, B. ramosus 21 and the hybrids 9 private bands (genetic overdispersion), probably resulting from the rearranged genomes. Based on its low genetic divergence index DW, the hybrid population seems to be at a young age. We argue that in the face of anthropogenic landscape transformations favouring secondary contacts, the hybrids may competitively replace the parental species in sympatric areas. © Polish Academy of Sciences and Jagiellonian University, Cracow 2015.},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The co-occurrence of hybrids and parental species in similar ecological niches poses a question on the role of traits additivity and overdispersion (emergence of new traits) in microevolutionary processes. We analysed genetic polymorphism of Bromus benekenii, B. ramosus and the spontaneous hybrid B. benekenii × B. ramosus in sympatric and allopatric parts of the species distribution in Europe, based on non-coding regions of the taxon genomes (ISSR genetic fingerprinting). We tested 68 individuals in 7 populations, including a hybrid population in N France. Altogether 233 polymorphic ISSR bands (loci) were obtained. We found that the parent species were genetically distinct and the hybrids had an additive pattern of ISSR bands found in the putative parental species (NMDS; STRUCTURE); however, there was evidence of introgression towards B. ramosus (NEWHYBRIDS; UPGMA classifications; Nei's D genetic distance). Bromus benekenii had 72, B. ramosus 21 and the hybrids 9 private bands (genetic overdispersion), probably resulting from the rearranged genomes. Based on its low genetic divergence index DW, the hybrid population seems to be at a young age. We argue that in the face of anthropogenic landscape transformations favouring secondary contacts, the hybrids may competitively replace the parental species in sympatric areas. © Polish Academy of Sciences and Jagiellonian University, Cracow 2015.
2014
Sutkowska, A.; Pasierbiński, A.; Warzecha, T.; Mitka, J.
Multiple cryptic refugia of forest grass Bromus benekenii in Europe as revealed by ISSR fingerprinting and species distribution modelling Journal Article
In: Plant Systematics and Evolution, vol. 300, no. 6, pp. 1437-1452, 2014, ISSN: 03782697, (12).
@article{2-s2.0-84902006460,
title = {Multiple cryptic refugia of forest grass Bromus benekenii in Europe as revealed by ISSR fingerprinting and species distribution modelling},
author = { A. Sutkowska and A. Pasierbiński and T. Warzecha and J. Mitka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84902006460&doi=10.1007%2fs00606-013-0972-x&partnerID=40&md5=aacf84467335236aed3b58bf9dc2387e},
doi = {10.1007/s00606-013-0972-x},
issn = {03782697},
year = {2014},
date = {2014-01-01},
journal = {Plant Systematics and Evolution},
volume = {300},
number = {6},
pages = {1437-1452},
publisher = {Springer-Verlag Wien},
abstract = {Despite not having been fully recognized, the cryptic northern refugia of temperate forest vegetation in Central and Western Europe are one of the most important in the Holocene history of the vegetation on the subcontinent. We have studied a forest grass Bromus benekenii in 39 populations in Central, Western and Southern Europe with the use of PCR-ISSR fingerprinting. The indices of genetic population diversity, multivariate, and Bayesian analyses, supplemented with species distribution modelling have enabled at least three putative cryptic northern refugial areas to be recognized: in Western Europe-the Central and Rhenish Massifs, in Central Europe-the Bohemia-Moravia region and in the Eastern/Western Carpathians. Central Poland is the regional genetic melting-pot where several migratory routes might have met. Southern Poland had a different postglacial history and was under the influence of an Eastern/Western Carpathian cryptic refugium. More forest species should be checked in a west-east gradient in Europe to corroborate the hypothesis on the Western European glacial refugia. © 2014 The Author(s).},
note = {12},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Despite not having been fully recognized, the cryptic northern refugia of temperate forest vegetation in Central and Western Europe are one of the most important in the Holocene history of the vegetation on the subcontinent. We have studied a forest grass Bromus benekenii in 39 populations in Central, Western and Southern Europe with the use of PCR-ISSR fingerprinting. The indices of genetic population diversity, multivariate, and Bayesian analyses, supplemented with species distribution modelling have enabled at least three putative cryptic northern refugial areas to be recognized: in Western Europe-the Central and Rhenish Massifs, in Central Europe-the Bohemia-Moravia region and in the Eastern/Western Carpathians. Central Poland is the regional genetic melting-pot where several migratory routes might have met. Southern Poland had a different postglacial history and was under the influence of an Eastern/Western Carpathian cryptic refugium. More forest species should be checked in a west-east gradient in Europe to corroborate the hypothesis on the Western European glacial refugia. © 2014 The Author(s).
2013
Sutkowska, A.; Pasierbiński, A.; Warzecha, T.; Mandal, A.; Mitka, J.
Refugial pattern of Bromus erectus in central Europe based on ISSR fingerprinting Journal Article
In: Acta Biologica Cracoviensia Series Botanica, vol. 55, no. 2, pp. 107-119, 2013, ISSN: 00015296, (7).
@article{2-s2.0-84894740357,
title = {Refugial pattern of Bromus erectus in central Europe based on ISSR fingerprinting},
author = { A. Sutkowska and A. Pasierbiński and T. Warzecha and A. Mandal and J. Mitka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894740357&doi=10.2478%2fabcsb-2013-0026&partnerID=40&md5=4f54e2562a4c757a2f105971f7d67dcc},
doi = {10.2478/abcsb-2013-0026},
issn = {00015296},
year = {2013},
date = {2013-01-01},
journal = {Acta Biologica Cracoviensia Series Botanica},
volume = {55},
number = {2},
pages = {107-119},
abstract = {We studied the thermophilous grass Bromus erectus in Central Europe to determine its pattern of population genetic structure and genetic diversity, using ISSR-PCR fingerprinting to analyze 200 individuals from 37 populations. We found three genetic groups with a clear geographic structure, based on a Bayesian approach. The first group occurred west and south of the Alps, the second east and north of the Alps, and the third was formed by four genetically depauperated populations in Germany. The populations from Germany formed a subset of the Bohemian-Moravian populations, with one private allele. Two differentiation centers, one in the Atlantic-Mediterranean and the second in the Pannonian-Balkan area, were recognized by species distribution modeling. The geographic distribution of the genetic groups coincides with the syntaxonomic split of the Festuco-Brometea class into the Festucetalia valesiaceae and Brometalia erecti orders. We found a statistically significant decrease in mean ISSR bands per individual from south to north, and to a lesser extent from the east to west. The former was explained by Holocene long-distance migrations from southern refugia, the latter by the difference in the gradient of anthropopression. We hypothesize a cryptic northern shelter of the species in Central Europe in the putative Moravian-Bohemian refugium. © Polish Academy of Sciences and Jagiellonian University, Cracow 2013.},
note = {7},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We studied the thermophilous grass Bromus erectus in Central Europe to determine its pattern of population genetic structure and genetic diversity, using ISSR-PCR fingerprinting to analyze 200 individuals from 37 populations. We found three genetic groups with a clear geographic structure, based on a Bayesian approach. The first group occurred west and south of the Alps, the second east and north of the Alps, and the third was formed by four genetically depauperated populations in Germany. The populations from Germany formed a subset of the Bohemian-Moravian populations, with one private allele. Two differentiation centers, one in the Atlantic-Mediterranean and the second in the Pannonian-Balkan area, were recognized by species distribution modeling. The geographic distribution of the genetic groups coincides with the syntaxonomic split of the Festuco-Brometea class into the Festucetalia valesiaceae and Brometalia erecti orders. We found a statistically significant decrease in mean ISSR bands per individual from south to north, and to a lesser extent from the east to west. The former was explained by Holocene long-distance migrations from southern refugia, the latter by the difference in the gradient of anthropopression. We hypothesize a cryptic northern shelter of the species in Central Europe in the putative Moravian-Bohemian refugium. © Polish Academy of Sciences and Jagiellonian University, Cracow 2013.
2009
Sutkowska, A.; Pasierbiński, A.
In: Fragmenta Floristica et Geobotanica Polonica, vol. 16, no. 2, pp. 281-295, 2009, ISSN: 1640629X.
@article{2-s2.0-84866097707,
title = {The origin of spontaneously spreading species Bromus carinalus (Poaceae) on ruderal and segetal sites in Poland [Pochodzenie spontanicznie rozprzestrzeniaja̧cego siȩ gatunku Bromus carinatus (Poaceae) na siedliskach ruderalnych i segetalnych w Polsce]},
author = { A. Sutkowska and A. Pasierbiński},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866097707&partnerID=40&md5=d4d3248873bb0ee57fac8d0eb8084fd4},
issn = {1640629X},
year = {2009},
date = {2009-01-01},
journal = {Fragmenta Floristica et Geobotanica Polonica},
volume = {16},
number = {2},
pages = {281-295},
abstract = {Molecular analysis of Bromus carinatus - an alien species in Polish flora, collected in ruderal and segetal sites in Poland, cv. 'Broma', and material from USDA Pullma collection was carried out using ISSR-PCR. We showed that most accessions from different sites shared the same band pattern with B.carinatus cv. 'Broma'. It suggests that B. carinatus cv. 'Broma' is spreading in Poland. Some rare individuals, present in investigated populations, probably originated from seeds that were brought accidentally from habitats of B. carinatus in other parts of Europe were detected.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Molecular analysis of Bromus carinatus - an alien species in Polish flora, collected in ruderal and segetal sites in Poland, cv. 'Broma', and material from USDA Pullma collection was carried out using ISSR-PCR. We showed that most accessions from different sites shared the same band pattern with B.carinatus cv. 'Broma'. It suggests that B. carinatus cv. 'Broma' is spreading in Poland. Some rare individuals, present in investigated populations, probably originated from seeds that were brought accidentally from habitats of B. carinatus in other parts of Europe were detected.