2023
Turnau, K.; Płachno, B. J.; Bień, P.; Świątek, P.; Dabrowski, P.; Kalaji, H. M.
Fungal symbionts impact cyanobacterial biofilm durability and photosynthetic efficiency Journal Article
In: Current Biology, vol. 33, no. 23, pp. 5257-5262.e3, 2023, ISSN: 09609822.
@article{2-s2.0-85178236171,
title = {Fungal symbionts impact cyanobacterial biofilm durability and photosynthetic efficiency},
author = { K. Turnau and B.J. Płachno and P. Bień and P. Świątek and P. Dabrowski and H.M. Kalaji},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85178236171&doi=10.1016%2fj.cub.2023.10.056&partnerID=40&md5=62a969ce1dc61a383d2b920272ec4b46},
doi = {10.1016/j.cub.2023.10.056},
issn = {09609822},
year = {2023},
date = {2023-01-01},
journal = {Current Biology},
volume = {33},
number = {23},
pages = {5257-5262.e3},
publisher = {Cell Press},
abstract = {Cyanobacteria contribute to over 25% of the world's net primary photosynthetic production and are pivotal in mitigating greenhouse gas emissions.1 This study unveils a previously unobserved symbiotic relationship between benthic cyanobacteria and fungi that have also adapted to life as a plant endophyte. The interaction suggests an initial phase of lichenization. We isolated Leptolyngbya frigida from the Naracauli stream, which emanates from abandoned Zn industrial waste in Sardinia. Seasonally, L. frigida participates in a biomineralization processes, mitigating the Zn transfer to rivers and, subsequently, the sea.2,3,4 L. frigida is a benthic cyanobacterium that establishes a biofilm on the stream bed. Notably, the area predominantly features Juncus acutus. From these roots, endophytic fungi were predominantly isolated as Clonostachys rosea, a fungus recognized for its biocontrol capabilities against plant pathogens. An intriguing observation was made when L. frigida was cultured with C. rosea on a low-carbohydrate agar medium: the fungal mycelium transformed into wall-less forms, a phenomenon not documented previously. In liquid environments, the resulting biofilm first settled at the container's bottom. Even upon rising to the surface, this biofilm remained pigment rich. Concurrently, a secondary biofilm began its formation at the bottom. These fungal-integrated biofilms displayed enhanced resilience and superior photosynthetic performance compared to those without fungal presence. Moreover, the symbiotic relationship significantly amplified O2 emission and CO2 sequestration by the biofilm. © 2023 Elsevier Inc.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Cyanobacteria contribute to over 25% of the world's net primary photosynthetic production and are pivotal in mitigating greenhouse gas emissions.1 This study unveils a previously unobserved symbiotic relationship between benthic cyanobacteria and fungi that have also adapted to life as a plant endophyte. The interaction suggests an initial phase of lichenization. We isolated Leptolyngbya frigida from the Naracauli stream, which emanates from abandoned Zn industrial waste in Sardinia. Seasonally, L. frigida participates in a biomineralization processes, mitigating the Zn transfer to rivers and, subsequently, the sea.2,3,4 L. frigida is a benthic cyanobacterium that establishes a biofilm on the stream bed. Notably, the area predominantly features Juncus acutus. From these roots, endophytic fungi were predominantly isolated as Clonostachys rosea, a fungus recognized for its biocontrol capabilities against plant pathogens. An intriguing observation was made when L. frigida was cultured with C. rosea on a low-carbohydrate agar medium: the fungal mycelium transformed into wall-less forms, a phenomenon not documented previously. In liquid environments, the resulting biofilm first settled at the container's bottom. Even upon rising to the surface, this biofilm remained pigment rich. Concurrently, a secondary biofilm began its formation at the bottom. These fungal-integrated biofilms displayed enhanced resilience and superior photosynthetic performance compared to those without fungal presence. Moreover, the symbiotic relationship significantly amplified O2 emission and CO2 sequestration by the biofilm. © 2023 Elsevier Inc.
2005
Przybyłowicz, W. J.; Mesjasz-Przybyłowicz, J.; Migula, P.; Nakonieczny, M.; Augustyniak, M.; Tarnawska, M.; Turnau, K.; Ryszka, P.; Orłowska, E.; Zubek, S.; Głowacka, E.
Micro-PIXE in ecophysiology Proceedings
vol. 34, no. 4, 2005, ISSN: 00498246, (31).
@proceedings{2-s2.0-22544482644,
title = {Micro-PIXE in ecophysiology},
author = { W.J. Przybyłowicz and J. Mesjasz-Przybyłowicz and P. Migula and M. Nakonieczny and M. Augustyniak and M. Tarnawska and K. Turnau and P. Ryszka and E. Orłowska and S. Zubek and E. Głowacka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-22544482644&doi=10.1002%2fxrs.826&partnerID=40&md5=09fb2d5d773bb5fd07fbd9d0bbaba0db},
doi = {10.1002/xrs.826},
issn = {00498246},
year = {2005},
date = {2005-01-01},
journal = {X-Ray Spectrometry},
volume = {34},
number = {4},
pages = {285-289},
abstract = {Two selected examples of PIXE microanalysis in ecophysiology are presented. Studies of heavy metal distributions in mycorrhizal and non-mycorrhizal roots of Plantago lanceolata showed different filtration mechanisms of Zn/Pb and Fe/Mn, both enabling plants to cope with metals present in the environment. Studies of the mechanism used by the beetle Chrysolina pardalina to eliminate excessive amounts of Ni revealed that Malpighian tubules are responsible for the elimination of this metal from the hemolymph. In both examples GeoPIXE software was used for true elemental mapping using the Dynamic Analysis method and analysis of spectra from selected micro-areas. Specimen thickness and matrix composition were obtained from proton backscattering spectra. Copyright © 2005 John Wiley & Sons, Ltd.},
note = {31},
keywords = {},
pubstate = {published},
tppubtype = {proceedings}
}
Two selected examples of PIXE microanalysis in ecophysiology are presented. Studies of heavy metal distributions in mycorrhizal and non-mycorrhizal roots of Plantago lanceolata showed different filtration mechanisms of Zn/Pb and Fe/Mn, both enabling plants to cope with metals present in the environment. Studies of the mechanism used by the beetle Chrysolina pardalina to eliminate excessive amounts of Ni revealed that Malpighian tubules are responsible for the elimination of this metal from the hemolymph. In both examples GeoPIXE software was used for true elemental mapping using the Dynamic Analysis method and analysis of spectra from selected micro-areas. Specimen thickness and matrix composition were obtained from proton backscattering spectra. Copyright © 2005 John Wiley & Sons, Ltd.
2004
Przybyłowicz, W. J.; Mesjasz-Przybyłowicz, J.; Migula, P.; Turnau, K.; Nakonieczny, M.; Augustyniak, M.; Głowacka, E.
Elemental microanalysis in ecophysiology using ion microbeam Proceedings
vol. 219-220, no. 1-4, 2004, ISSN: 0168583X, (25).
@proceedings{2-s2.0-2342598370,
title = {Elemental microanalysis in ecophysiology using ion microbeam},
author = { W.J. Przybyłowicz and J. Mesjasz-Przybyłowicz and P. Migula and K. Turnau and M. Nakonieczny and M. Augustyniak and E. Głowacka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2342598370&doi=10.1016%2fj.nimb.2004.01.028&partnerID=40&md5=abd076cdbfc62dbcb2a646ea9353e747},
doi = {10.1016/j.nimb.2004.01.028},
issn = {0168583X},
year = {2004},
date = {2004-01-01},
journal = {Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms},
volume = {219-220},
number = {1-4},
pages = {57-66},
abstract = {A few recent applications of elemental microanalysis based on proton beam in ecophysiology and ecotoxicology are shown. They are related to biofiltering capabilities of mycorrhiza (symbiosis between fungi and plant roots) and to plant-insect herbivore interactions. The reported results were obtained at iThemba LABS, South Africa. PIXE and BS techniques were simultaneously used. True elemental maps were generated using a VMS version and PC version of GeoPIXE (GeoPIXE I and II). Further analysis was performed using PIXE and BS spectra extracted from list-mode data and corresponding to specific organs of an insect or a plant. © 2004 Elsevier B.V. All rights reserved.},
note = {25},
keywords = {},
pubstate = {published},
tppubtype = {proceedings}
}
A few recent applications of elemental microanalysis based on proton beam in ecophysiology and ecotoxicology are shown. They are related to biofiltering capabilities of mycorrhiza (symbiosis between fungi and plant roots) and to plant-insect herbivore interactions. The reported results were obtained at iThemba LABS, South Africa. PIXE and BS techniques were simultaneously used. True elemental maps were generated using a VMS version and PC version of GeoPIXE (GeoPIXE I and II). Further analysis was performed using PIXE and BS spectra extracted from list-mode data and corresponding to specific organs of an insect or a plant. © 2004 Elsevier B.V. All rights reserved.