2020
Pociecha, E.; Janeczko, A.; Dziurka, M. A.; Gruszka, D.
In: Journal of Plant Growth Regulation, vol. 39, no. 4, pp. 1625-1637, 2020, ISSN: 07217595, (2).
@article{2-s2.0-85087511365,
title = {Disturbances in the Biosynthesis or Signalling of Brassinosteroids That Are Caused by Mutations in the HvDWARF, HvCPD and HvBRI1 Genes Increase the Tolerance of Barley to the Deacclimation Process},
author = { E. Pociecha and A. Janeczko and M.A. Dziurka and D. Gruszka},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087511365&doi=10.1007%2fs00344-020-10183-4&partnerID=40&md5=0e561928f2ea830133c754918015b828},
doi = {10.1007/s00344-020-10183-4},
issn = {07217595},
year = {2020},
date = {2020-01-01},
journal = {Journal of Plant Growth Regulation},
volume = {39},
number = {4},
pages = {1625-1637},
publisher = {Springer},
abstract = {Tolerance to deacclimation is an important physiological feature in plants in the face of global warming, which is resulting in incidents of increases in winter temperatures. The aim of the work was to explore how disturbances in the signalling and synthesis of brassinosteroids (BR) influence the deacclimation tolerance of barley. One group of mutants and their reference cultivars (Bowman and Delisa) was cold-acclimated, deacclimated and then tested for frost tolerance at − 12 °C. After cold acclimation, the second group of plants was additionally exposed to frost (− 6 °C) and then, deacclimated and tested for frost tolerance at − 12 °C. The deacclimated brassinosteroid mutants were characterised by an increased tolerance to frost, and consequently, had a higher tolerance to deacclimation than their wild-type cultivars. The mechanism of this phenomenon may be partly explained by analysing the hormonal homeostasis in the crowns. For all of the tested plants, a characteristic feature of the response to the deacclimation phase was an increase in the growth-promoting hormones and abscisic acid compared to the cold acclimation phase. The increase was greater in the BR-deficient (BW084) and BR-insensitive (BW312) mutants compared to the Bowman reference cultivar. Mutant 522DK was characterised by a lower accumulation of total cytokinins and gibberellins as well as an enhanced auxin deactivation compared to the Delisa. In the second group, when the plants were exposed to a temperature of − 6 °C before deacclimation, the hormonal homeostasis was further altered in both the mutants and reference cultivars, but all of the mutants had a higher frost tolerance than the wild types. © 2020, The Author(s).},
note = {2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Tolerance to deacclimation is an important physiological feature in plants in the face of global warming, which is resulting in incidents of increases in winter temperatures. The aim of the work was to explore how disturbances in the signalling and synthesis of brassinosteroids (BR) influence the deacclimation tolerance of barley. One group of mutants and their reference cultivars (Bowman and Delisa) was cold-acclimated, deacclimated and then tested for frost tolerance at − 12 °C. After cold acclimation, the second group of plants was additionally exposed to frost (− 6 °C) and then, deacclimated and tested for frost tolerance at − 12 °C. The deacclimated brassinosteroid mutants were characterised by an increased tolerance to frost, and consequently, had a higher tolerance to deacclimation than their wild-type cultivars. The mechanism of this phenomenon may be partly explained by analysing the hormonal homeostasis in the crowns. For all of the tested plants, a characteristic feature of the response to the deacclimation phase was an increase in the growth-promoting hormones and abscisic acid compared to the cold acclimation phase. The increase was greater in the BR-deficient (BW084) and BR-insensitive (BW312) mutants compared to the Bowman reference cultivar. Mutant 522DK was characterised by a lower accumulation of total cytokinins and gibberellins as well as an enhanced auxin deactivation compared to the Delisa. In the second group, when the plants were exposed to a temperature of − 6 °C before deacclimation, the hormonal homeostasis was further altered in both the mutants and reference cultivars, but all of the mutants had a higher frost tolerance than the wild types. © 2020, The Author(s).
Surówka, E.; Potocka, I. W.; Dziurka, M. A.; Wróbel-Marek, J.; Kurczyńska, E. U.; Żur, I.; Maksymowicz, A.; Gajewska, E.; Miszalski, Z.
Tocopherols mutual balance is a key player for maintaining Arabidopsis thaliana growth under salt stress Journal Article
In: Plant Physiology and Biochemistry, vol. 156, pp. 369-383, 2020, ISSN: 09819428, (8).
@article{2-s2.0-85091670721,
title = {Tocopherols mutual balance is a key player for maintaining Arabidopsis thaliana growth under salt stress},
author = { E. Surówka and I.W. Potocka and M.A. Dziurka and J. Wróbel-Marek and E.U. Kurczyńska and I. Żur and A. Maksymowicz and E. Gajewska and Z. Miszalski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091670721&doi=10.1016%2fj.plaphy.2020.09.008&partnerID=40&md5=178d47e55217a42bf706da8ce75b97d7},
doi = {10.1016/j.plaphy.2020.09.008},
issn = {09819428},
year = {2020},
date = {2020-01-01},
journal = {Plant Physiology and Biochemistry},
volume = {156},
pages = {369-383},
publisher = {Elsevier Masson s.r.l.},
abstract = {Enhanced channeling carbon through pathways: shikimate/chorismate, benzenoid-phenylopropanoid or 2-C-methyl-D-erythritol 4-phosphate (MEP) provides a multitude of secondary metabolites and cell wall components and allows plants response to environmental stresses. Through the biosynthetic pathways, different secondary metabolites, like tocopherols (TCs), are bind to mutual dependencies and metabolic loops, that are not yet fully understood. We compared, in parallel, the influence of α- and γ-TCs on metabolites involved in osmoprotective/antioxidative response, and physico-chemical modification of plasma membrane and cell wall. We studied Arabidopsis thaliana Columbia ecotype (WT), mutant vte1 deficient in α- and γ-TCs, mutant vte4 over-accumulating γ-TC instead of α-TC, and transgenic line tmt over-accumulating α-TC; exposed to NaCl. The results indicate that salt stress activates β-carboxylation processes in WT plants and in plants with altered TCs accumulation. In α-TC-deficient plants, NaCl causes ACC decrease, but does not change SA, whose concentration remains higher than in α-TC accumulating plants. α/γ-TCs contents influence carbohydrates, poliamines, phenolic (caffeic; ferrulic; cinnamic) acids accumulation patterns. Salinity results in increased detection of the LM5 galactan and LM19 homogalacturonan epitopes in α-TC accumulating plants, and the LM6 arabinan and MAC207 AGP epitopes in α-TC deficient mutants. Parallel, plants with altered TCs composition show decreased both the cell turgor and elastic modulus determined at the individual cell level. α-TC deficient plants reveal lower values of cell turgor and elastic modulus, but higher cell hydraulic conductivity than α-TC accumulating plants. Under salt stress, α-TC shows stronger regulatory effect than γ-TC through the impact on chloroplastic biosynthetic pathways and ROS/osmotic-modulating compounds. © 2020 Elsevier Masson SAS},
note = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Enhanced channeling carbon through pathways: shikimate/chorismate, benzenoid-phenylopropanoid or 2-C-methyl-D-erythritol 4-phosphate (MEP) provides a multitude of secondary metabolites and cell wall components and allows plants response to environmental stresses. Through the biosynthetic pathways, different secondary metabolites, like tocopherols (TCs), are bind to mutual dependencies and metabolic loops, that are not yet fully understood. We compared, in parallel, the influence of α- and γ-TCs on metabolites involved in osmoprotective/antioxidative response, and physico-chemical modification of plasma membrane and cell wall. We studied Arabidopsis thaliana Columbia ecotype (WT), mutant vte1 deficient in α- and γ-TCs, mutant vte4 over-accumulating γ-TC instead of α-TC, and transgenic line tmt over-accumulating α-TC; exposed to NaCl. The results indicate that salt stress activates β-carboxylation processes in WT plants and in plants with altered TCs accumulation. In α-TC-deficient plants, NaCl causes ACC decrease, but does not change SA, whose concentration remains higher than in α-TC accumulating plants. α/γ-TCs contents influence carbohydrates, poliamines, phenolic (caffeic; ferrulic; cinnamic) acids accumulation patterns. Salinity results in increased detection of the LM5 galactan and LM19 homogalacturonan epitopes in α-TC accumulating plants, and the LM6 arabinan and MAC207 AGP epitopes in α-TC deficient mutants. Parallel, plants with altered TCs composition show decreased both the cell turgor and elastic modulus determined at the individual cell level. α-TC deficient plants reveal lower values of cell turgor and elastic modulus, but higher cell hydraulic conductivity than α-TC accumulating plants. Under salt stress, α-TC shows stronger regulatory effect than γ-TC through the impact on chloroplastic biosynthetic pathways and ROS/osmotic-modulating compounds. © 2020 Elsevier Masson SAS
Gruszka, D.; Pociecha, E.; Jurczyk, B.; Dziurka, M. A.; Oliwa, J.; Sadura, I.; Janeczko, A.
Insights into metabolic reactions of semi-dwarf, barley brassinosteroid mutants to drought Journal Article
In: International Journal of Molecular Sciences, vol. 21, no. 14, pp. 1-30, 2020, ISSN: 16616596, (7).
@article{2-s2.0-85088679534,
title = {Insights into metabolic reactions of semi-dwarf, barley brassinosteroid mutants to drought},
author = { D. Gruszka and E. Pociecha and B. Jurczyk and M.A. Dziurka and J. Oliwa and I. Sadura and A. Janeczko},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088679534&doi=10.3390%2fijms21145096&partnerID=40&md5=2dd3b0e0f609c31e7f94c40aca810f20},
doi = {10.3390/ijms21145096},
issn = {16616596},
year = {2020},
date = {2020-01-01},
journal = {International Journal of Molecular Sciences},
volume = {21},
number = {14},
pages = {1-30},
publisher = {MDPI AG},
abstract = {The roles of endogenous brassinosteroids (BRs) in the modulation of reaction to drought and genetic regulation of this process are still obscure. In this study, a multidirectional analysis was performed on semi-dwarf barley (Hordeum vulgare) Near-Isogenic Lines (NILs) and the reference cultivar “Bowman” to get insights into various aspects of metabolic reaction to drought. The NILs are defective in BR biosynthesis or signaling and displayed an enhanced tolerance to drought. The BR metabolism perturbations affected the glucose and fructose accumulation under the control and stress conditions. The BR metabolism abnormalities negatively affected the sucrose accumulation as well. However, during drought, the BR-deficient NILs accumulated higher contents of sucrose than the “Bowman” cultivar. Under the control conditions, accumulation of transcripts encoding antioxidant enzymes ascorbate peroxidase (HvAPX) and superoxide dismutase (HvSOD) was BR-dependent. However, during drought, the accumulation of HvAPX transcript was BR-dependent, whereas accumulations of transcripts encoding catalase (HvCAT) and HvSOD were not affected by the BR metabolism perturbations. The obtained results reveal a significant role of BRs in regulation of the HvAPX and HvCAT enzymatic activities under control conditions and the HvAPX and HvSOD activities during physiological reactions to drought. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {7},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The roles of endogenous brassinosteroids (BRs) in the modulation of reaction to drought and genetic regulation of this process are still obscure. In this study, a multidirectional analysis was performed on semi-dwarf barley (Hordeum vulgare) Near-Isogenic Lines (NILs) and the reference cultivar “Bowman” to get insights into various aspects of metabolic reaction to drought. The NILs are defective in BR biosynthesis or signaling and displayed an enhanced tolerance to drought. The BR metabolism perturbations affected the glucose and fructose accumulation under the control and stress conditions. The BR metabolism abnormalities negatively affected the sucrose accumulation as well. However, during drought, the BR-deficient NILs accumulated higher contents of sucrose than the “Bowman” cultivar. Under the control conditions, accumulation of transcripts encoding antioxidant enzymes ascorbate peroxidase (HvAPX) and superoxide dismutase (HvSOD) was BR-dependent. However, during drought, the accumulation of HvAPX transcript was BR-dependent, whereas accumulations of transcripts encoding catalase (HvCAT) and HvSOD were not affected by the BR metabolism perturbations. The obtained results reveal a significant role of BRs in regulation of the HvAPX and HvCAT enzymatic activities under control conditions and the HvAPX and HvSOD activities during physiological reactions to drought. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
2019
Janeczko, A.; Saja, D.; Dziurka, M. A.; Gullner, G.; Kornaś, A.; Skoczowski, A.; Gruszka, D.; Barna, B.
Brassinosteroid deficiency caused by the mutation of the HvDWARF gene influences the reactions of barley to powdery mildew Journal Article
In: Physiological and Molecular Plant Pathology, vol. 108, 2019, ISSN: 08855765, (3).
@article{2-s2.0-85073497006,
title = {Brassinosteroid deficiency caused by the mutation of the HvDWARF gene influences the reactions of barley to powdery mildew},
author = { A. Janeczko and D. Saja and M.A. Dziurka and G. Gullner and A. Kornaś and A. Skoczowski and D. Gruszka and B. Barna},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073497006&doi=10.1016%2fj.pmpp.2019.101438&partnerID=40&md5=04895c95ba6734203c368e87d4015311},
doi = {10.1016/j.pmpp.2019.101438},
issn = {08855765},
year = {2019},
date = {2019-01-01},
journal = {Physiological and Molecular Plant Pathology},
volume = {108},
publisher = {Academic Press},
abstract = {This study investigated the impact of mutation in the HvDWARF gene, encoding C6-oxidase involved in brassinosteroid (BR) biosynthesis, on disease resistance of barley against the fungal pathogen powdery mildew (Blumeria graminis f. sp. hordei). The mutation results in significant BR deficiency in leaf tissue. Since increase in the BR content by their exogenous application was reported to improve plant resistance to viral, bacterial and fungal infection, we wanted to find out, if lowered content of BR may affect plant resistance to this pathogen. The 527DK mutant carrying the aforementioned mutation and its respective wild type Delisa were inoculated by powdery mildew fungus (Bgh; race A6). However, no symptoms of disease were visually noted neither on 527DK nor on Delisa. Microscopic observations showed that after germination of the conidia the germ tubes started to penetrate, developed appressorium but could not develop further in the two genotypes. The cell wall thickening (papillae) around the penetration was only visible. However, inoculated plants showed changes in some of parameters describing photosynthetic efficiency or water relations. Similarly, alterations in hormonal homeostasis not only between wild type and mutant, but between control and infected plants were observed. Inoculation with the pathogen induced especially accumulation of compounds from auxin group (indole-3-carboxylic acid; oxindole-3-acetic acid; 4-chloroindole-3-acetic acid and 5-chloroindole-3-acetic acid). Interestingly, the stronger effect was observed in plants with mutation in the HvDWARF gene. Taking into account that the HvDWARF gene mutation finally did not affect resistance to powdery mildew (no symptoms appearance), we suggest that the conclusion of our study may be of importance for breeding of barley. © 2019 Elsevier Ltd},
note = {3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study investigated the impact of mutation in the HvDWARF gene, encoding C6-oxidase involved in brassinosteroid (BR) biosynthesis, on disease resistance of barley against the fungal pathogen powdery mildew (Blumeria graminis f. sp. hordei). The mutation results in significant BR deficiency in leaf tissue. Since increase in the BR content by their exogenous application was reported to improve plant resistance to viral, bacterial and fungal infection, we wanted to find out, if lowered content of BR may affect plant resistance to this pathogen. The 527DK mutant carrying the aforementioned mutation and its respective wild type Delisa were inoculated by powdery mildew fungus (Bgh; race A6). However, no symptoms of disease were visually noted neither on 527DK nor on Delisa. Microscopic observations showed that after germination of the conidia the germ tubes started to penetrate, developed appressorium but could not develop further in the two genotypes. The cell wall thickening (papillae) around the penetration was only visible. However, inoculated plants showed changes in some of parameters describing photosynthetic efficiency or water relations. Similarly, alterations in hormonal homeostasis not only between wild type and mutant, but between control and infected plants were observed. Inoculation with the pathogen induced especially accumulation of compounds from auxin group (indole-3-carboxylic acid; oxindole-3-acetic acid; 4-chloroindole-3-acetic acid and 5-chloroindole-3-acetic acid). Interestingly, the stronger effect was observed in plants with mutation in the HvDWARF gene. Taking into account that the HvDWARF gene mutation finally did not affect resistance to powdery mildew (no symptoms appearance), we suggest that the conclusion of our study may be of importance for breeding of barley. © 2019 Elsevier Ltd
Sadura, I.; Pociecha, E.; Dziurka, M. A.; Oklestkova, J.; Novák, O.; Gruszka, D.; Janeczko, A.
In: Journal of Plant Growth Regulation, vol. 38, no. 3, pp. 1062-1081, 2019, ISSN: 07217595, (22).
@article{2-s2.0-85061388993,
title = {Mutations in the HvDWARF, HvCPD and HvBRI1 Genes-Involved in Brassinosteroid Biosynthesis/Signalling: Altered Photosynthetic Efficiency, Hormonal Homeostasis and Tolerance to High/Low Temperatures in Barley},
author = { I. Sadura and E. Pociecha and M.A. Dziurka and J. Oklestkova and O. Novák and D. Gruszka and A. Janeczko},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061388993&doi=10.1007%2fs00344-019-09914-z&partnerID=40&md5=23cabff4b2b733c629610fabc18a4a6a},
doi = {10.1007/s00344-019-09914-z},
issn = {07217595},
year = {2019},
date = {2019-01-01},
journal = {Journal of Plant Growth Regulation},
volume = {38},
number = {3},
pages = {1062-1081},
publisher = {Springer New York LLC},
abstract = {Brassinosteroids (BR) are steroid phytohormones that are involved in the growth and stress response in plants, but the precise mechanisms of their action are still being discovered. In our study we have used BR-deficient barley mutants 522DK and BW084 (which carry missense mutations in the HvDWARF and HvCPD genes; respectively). We have also used a BR-signalling mutant that harbors missense substitutions in the HvBRI1 gene. Our aim was (1) to find out if the content of phytohormones in the mutants grown at 20 °C is different than in the wild types and whether/how the content of phytohormones changes after plant acclimation at temperatures of 5 °C and 27 °C?, (2) to characterise the effectiveness of the light reactions of photosynthesis of the barley mutants in comparison to wild types at various temperatures, and (3) to verify the impact of mutations on the tolerance of barley to high and low temperatures. Hormonal characteristics of the BR mutants of barley show the complexity of the interactions between BR and other plant hormones that are additionally modified by temperature and possibly by other factors. The results suggest the participation of BR in auxin catabolism. Further, BR appears to play a role in maintaining the ABA–ABAGlc balance. As for the gibberellin content in plants at a temperature of 20 °C, more in-depth studies will be required to explain the contradictory effects regarding the accumulation of GA3, GA4 and GA5, which appears to be dependent on the type of mutation and connected to the BR level. A fast-kinetic chlorophyll a fluorescence analysis has revealed that the mutants had lower values of energy absorption than the wild types, but the values of the energy transferred via the electron-transport chain was maintained at the wild-type level. We presumed that BR are involved in regulating plant acclimation to extreme (low/high) temperatures, thus the BR-deficient and BR-signalling mutants should be less tolerant to low/high temperatures when compared to the wild types. Unexpectedly, all of the mutants showed a higher tolerance to high temperatures than the wild types. The BW084 and BW312 mutants were less tolerant to frost than the wild type, but 522DK had a similar frost tolerance as the reference wild-type cultivar. © 2019, The Author(s).},
note = {22},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Brassinosteroids (BR) are steroid phytohormones that are involved in the growth and stress response in plants, but the precise mechanisms of their action are still being discovered. In our study we have used BR-deficient barley mutants 522DK and BW084 (which carry missense mutations in the HvDWARF and HvCPD genes; respectively). We have also used a BR-signalling mutant that harbors missense substitutions in the HvBRI1 gene. Our aim was (1) to find out if the content of phytohormones in the mutants grown at 20 °C is different than in the wild types and whether/how the content of phytohormones changes after plant acclimation at temperatures of 5 °C and 27 °C?, (2) to characterise the effectiveness of the light reactions of photosynthesis of the barley mutants in comparison to wild types at various temperatures, and (3) to verify the impact of mutations on the tolerance of barley to high and low temperatures. Hormonal characteristics of the BR mutants of barley show the complexity of the interactions between BR and other plant hormones that are additionally modified by temperature and possibly by other factors. The results suggest the participation of BR in auxin catabolism. Further, BR appears to play a role in maintaining the ABA–ABAGlc balance. As for the gibberellin content in plants at a temperature of 20 °C, more in-depth studies will be required to explain the contradictory effects regarding the accumulation of GA3, GA4 and GA5, which appears to be dependent on the type of mutation and connected to the BR level. A fast-kinetic chlorophyll a fluorescence analysis has revealed that the mutants had lower values of energy absorption than the wild types, but the values of the energy transferred via the electron-transport chain was maintained at the wild-type level. We presumed that BR are involved in regulating plant acclimation to extreme (low/high) temperatures, thus the BR-deficient and BR-signalling mutants should be less tolerant to low/high temperatures when compared to the wild types. Unexpectedly, all of the mutants showed a higher tolerance to high temperatures than the wild types. The BW084 and BW312 mutants were less tolerant to frost than the wild type, but 522DK had a similar frost tolerance as the reference wild-type cultivar. © 2019, The Author(s).
2018
Gudyś, K.; Guzy-Wróbelska, J.; Janiak, A.; Dziurka, M. A.; Ostrowska, A.; Hura, K.; Jurczyk, B.; Żmuda, K.; Grzybkowska, D.; Śróbka, J.; Urban, W.; Biesaga-Kościelniak, J.; Filek, M.; Kościelniak, J.; Mikołajczak, K.; Ogrodowicz, P.; Krystkowiak, K.; Kuczyńska, A.; Krajewski, P.; Szarejko, I.
In: Frontiers in Plant Science, vol. 9, 2018, ISSN: 1664462X, (22).
@article{2-s2.0-85049182046,
title = {Prioritization of candidate genes in qtl regions for physiological and biochemical traits underlying drought response in barley (Hordeum vulgare L.)},
author = { K. Gudyś and J. Guzy-Wróbelska and A. Janiak and M.A. Dziurka and A. Ostrowska and K. Hura and B. Jurczyk and K. Żmuda and D. Grzybkowska and J. Śróbka and W. Urban and J. Biesaga-Kościelniak and M. Filek and J. Kościelniak and K. Mikołajczak and P. Ogrodowicz and K. Krystkowiak and A. Kuczyńska and P. Krajewski and I. Szarejko},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049182046&doi=10.3389%2ffpls.2018.00769&partnerID=40&md5=ee4f20bff713e6791812c6e0fb2a84e8},
doi = {10.3389/fpls.2018.00769},
issn = {1664462X},
year = {2018},
date = {2018-01-01},
journal = {Frontiers in Plant Science},
volume = {9},
publisher = {Frontiers Media S.A.},
abstract = {Drought is one of the most adverse abiotic factors limiting growth and productivity of them were genes encoding antioxidants, carboxylic acid biosynthesis enzymes, heat shock proteins, small auxin up-regulated RNAs, nitric oxide synthase, ATP sulfurylases, and proteins involved in regulation of flowering time. This global approach may be proposed for identification of new CGs that underlies QTLs responsible for complex traits. crops. Among them is barley, ranked fourth cereal worldwide in terms of harvested acreage and production. Plants have evolved various mechanisms to cope with water deficit at different biological levels, but there is an enormous challenge to decipher genes responsible for particular complex phenotypic traits, in order to develop drought tolerant crops. This work presents a comprehensive approach for elucidation of molecular mechanisms of drought tolerance in barley at the seedling stage of development. The study includes mapping of QTLs for physiological and biochemical traits associated with drought tolerance on a high-density function map, projection of QTL confidence intervals on barley physical map, and the retrievement of positional candidate genes (CGs), followed by their prioritization based on Gene Ontology (GO) enrichment analysis. A total of 64 QTLs for 25 physiological and biochemical traits that describe plant water status, photosynthetic efficiency, osmoprotectant and hormone content, as well as antioxidant activity, were positioned on a consensus map, constructed using RIL populations developed from the crosses between European and Syrian genotypes. The map contained a total of 875 SNP, SSR and CGs, spanning 941.86 cM with resolution of 1.1 cM. For the first time, QTLs for ethylene, glucose, sucrose, maltose, raffinose, a-tocopherol, g-tocotrienol content, and catalase activity, have been mapped in barley. Based on overlapping confidence intervals of QTLs, 11 hotspots were identified that enclosedmore than 60%ofmapped QTLs. Genetic and physicalmap integration allowed the identification of 1,101 positional CGs within the confidence intervals of drought response-specific QTLs. Prioritization resulted in the designation of 143 CGs, among them were genes encoding antioxidants, carboxylic acid biosynthesis enzymes, heat shock proteins, small auxin up-regulated RNAs, nitric oxide synthase, ATP sulfurylases, and proteins involved in regulation of flowering time. This global approach may be proposed for identification of new CGs that underlies QTLs responsible for complex traits. © 2018 Gudys, Guzy-Wrobelska, Janiak, Dziurka, Ostrowska, Hura, Jurczyk, Żmuda, Grzybkowska, Śróbka, Urban, Biesaga-Koscielniak, Filek, Koscielniak, Mikołajczak, Ogrodowicz, Krystkowiak, Kuczyńska, Krajewski and Szarejko.},
note = {22},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Drought is one of the most adverse abiotic factors limiting growth and productivity of them were genes encoding antioxidants, carboxylic acid biosynthesis enzymes, heat shock proteins, small auxin up-regulated RNAs, nitric oxide synthase, ATP sulfurylases, and proteins involved in regulation of flowering time. This global approach may be proposed for identification of new CGs that underlies QTLs responsible for complex traits. crops. Among them is barley, ranked fourth cereal worldwide in terms of harvested acreage and production. Plants have evolved various mechanisms to cope with water deficit at different biological levels, but there is an enormous challenge to decipher genes responsible for particular complex phenotypic traits, in order to develop drought tolerant crops. This work presents a comprehensive approach for elucidation of molecular mechanisms of drought tolerance in barley at the seedling stage of development. The study includes mapping of QTLs for physiological and biochemical traits associated with drought tolerance on a high-density function map, projection of QTL confidence intervals on barley physical map, and the retrievement of positional candidate genes (CGs), followed by their prioritization based on Gene Ontology (GO) enrichment analysis. A total of 64 QTLs for 25 physiological and biochemical traits that describe plant water status, photosynthetic efficiency, osmoprotectant and hormone content, as well as antioxidant activity, were positioned on a consensus map, constructed using RIL populations developed from the crosses between European and Syrian genotypes. The map contained a total of 875 SNP, SSR and CGs, spanning 941.86 cM with resolution of 1.1 cM. For the first time, QTLs for ethylene, glucose, sucrose, maltose, raffinose, a-tocopherol, g-tocotrienol content, and catalase activity, have been mapped in barley. Based on overlapping confidence intervals of QTLs, 11 hotspots were identified that enclosedmore than 60%ofmapped QTLs. Genetic and physicalmap integration allowed the identification of 1,101 positional CGs within the confidence intervals of drought response-specific QTLs. Prioritization resulted in the designation of 143 CGs, among them were genes encoding antioxidants, carboxylic acid biosynthesis enzymes, heat shock proteins, small auxin up-regulated RNAs, nitric oxide synthase, ATP sulfurylases, and proteins involved in regulation of flowering time. This global approach may be proposed for identification of new CGs that underlies QTLs responsible for complex traits. © 2018 Gudys, Guzy-Wrobelska, Janiak, Dziurka, Ostrowska, Hura, Jurczyk, Żmuda, Grzybkowska, Śróbka, Urban, Biesaga-Koscielniak, Filek, Koscielniak, Mikołajczak, Ogrodowicz, Krystkowiak, Kuczyńska, Krajewski and Szarejko.
Gruszka, D.; Janeczko, A.; Dziurka, M. A.; Pociecha, E.; Fodor, J.
Non-enzymatic antioxidant accumulations in BR-deficient and BR-insensitive barley mutants under control and drought conditions Journal Article
In: Physiologia Plantarum, vol. 163, no. 2, pp. 155-169, 2018, ISSN: 00319317, (13).
@article{2-s2.0-85048310777,
title = {Non-enzymatic antioxidant accumulations in BR-deficient and BR-insensitive barley mutants under control and drought conditions},
author = { D. Gruszka and A. Janeczko and M.A. Dziurka and E. Pociecha and J. Fodor},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048310777&doi=10.1111%2fppl.12674&partnerID=40&md5=50ce3d9eeeeb55fcde27e5453bc9484b},
doi = {10.1111/ppl.12674},
issn = {00319317},
year = {2018},
date = {2018-01-01},
journal = {Physiologia Plantarum},
volume = {163},
number = {2},
pages = {155-169},
publisher = {Blackwell Publishing Ltd},
abstract = {Drought is one of the most adverse stresses that affect plant growth and yield. Disturbances in metabolic activity resulting from drought cause overproduction of reactive oxygen species. It is postulated that brassinosteroids (BRs) regulate plant tolerance to the stress conditions, but the underlying mechanisms remain largely unknown. An involvement of endogenous BRs in regulation of the antioxidant homeostasis is not fully clarified either. Therefore, the aim of this study was to elucidate the role of endogenous BRs in regulation of non-enzymatic antioxidants in barley (Hordeum vulgare) under control and drought conditions. The plant material included the ‘Bowman’ cultivar and a group of semi-dwarf near-isogenic lines (NILs), representing mutants deficient in BR biosynthesis or signaling. In general, accumulations of 11 compounds representing various types of non-enzymatic antioxidants were analyzed under both conditions. The analyses of accumulations of reduced and oxidized forms of ascorbate indicated that the BR mutants contain significantly higher contents of dehydroascorbic acid under drought conditions when compared with the ‘Bowman’ cultivar. The analysis of glutathione accumulation indicated that under the control conditions the BR-insensitive NILs contained significantly lower concentrations of this antioxidant when compared with the rest of genotypes. Therefore, we postulate that BR sensitivity is required for normal accumulation of glutathione. A complete accumulation profile of various tocopherols indicated that functional BR biosynthesis and signaling are required for their normal accumulation under both conditions. Results of this study provided an insight into the role of endogenous BRs in regulation of the non-enzymatic antioxidant homeostasis. © 2017 Scandinavian Plant Physiology Society},
note = {13},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Drought is one of the most adverse stresses that affect plant growth and yield. Disturbances in metabolic activity resulting from drought cause overproduction of reactive oxygen species. It is postulated that brassinosteroids (BRs) regulate plant tolerance to the stress conditions, but the underlying mechanisms remain largely unknown. An involvement of endogenous BRs in regulation of the antioxidant homeostasis is not fully clarified either. Therefore, the aim of this study was to elucidate the role of endogenous BRs in regulation of non-enzymatic antioxidants in barley (Hordeum vulgare) under control and drought conditions. The plant material included the ‘Bowman’ cultivar and a group of semi-dwarf near-isogenic lines (NILs), representing mutants deficient in BR biosynthesis or signaling. In general, accumulations of 11 compounds representing various types of non-enzymatic antioxidants were analyzed under both conditions. The analyses of accumulations of reduced and oxidized forms of ascorbate indicated that the BR mutants contain significantly higher contents of dehydroascorbic acid under drought conditions when compared with the ‘Bowman’ cultivar. The analysis of glutathione accumulation indicated that under the control conditions the BR-insensitive NILs contained significantly lower concentrations of this antioxidant when compared with the rest of genotypes. Therefore, we postulate that BR sensitivity is required for normal accumulation of glutathione. A complete accumulation profile of various tocopherols indicated that functional BR biosynthesis and signaling are required for their normal accumulation under both conditions. Results of this study provided an insight into the role of endogenous BRs in regulation of the non-enzymatic antioxidant homeostasis. © 2017 Scandinavian Plant Physiology Society
2016
Gruszka, D.; Janeczko, A.; Dziurka, M. A.; Pociecha, E.; Oklestkova, J.; Szarejko, I.
Barley brassinosteroid mutants provide an insight into phytohormonal homeostasis in plant reaction to drought stress Journal Article
In: Frontiers in Plant Science, vol. 7, no. DECEMBER2016, 2016, ISSN: 1664462X, (40).
@article{2-s2.0-85007386454,
title = {Barley brassinosteroid mutants provide an insight into phytohormonal homeostasis in plant reaction to drought stress},
author = { D. Gruszka and A. Janeczko and M.A. Dziurka and E. Pociecha and J. Oklestkova and I. Szarejko},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85007386454&doi=10.3389%2ffpls.2016.01824&partnerID=40&md5=f3b1faf0ff5e91bb21516d557d2d8f98},
doi = {10.3389/fpls.2016.01824},
issn = {1664462X},
year = {2016},
date = {2016-01-01},
journal = {Frontiers in Plant Science},
volume = {7},
number = {DECEMBER2016},
publisher = {Frontiers Media S.A.},
abstract = {Brassinosteroids (BRs) are a class of steroid phytohormones, which regulate various processes of morphogenesis and physiology—from seed development to regulation of flowering and senescence. An accumulating body of evidence indicates that BRs take part in regulation of physiological reactions to various stress conditions, including drought. Many of the physiological functions of BRs are regulated by a complicated, and not fully elucidated network of interactions with metabolic pathways of other phytohormones. Therefore, the aim of this study was to characterize phytohormonal homeostasis in barley (Hordeum vulgare) in reaction to drought and validate role of BRs in regulation of this process. Material of this study included the barley cultivar “Bowman” and five Near-Isogenic Lines (NILs) representing characterized semi-dwarf mutants of several genes encoding enzymes participating in BR biosynthesis and signaling. Analysis of endogenous BRs concentrations in these NILs confirmed that their phenotypes result from abnormalities in BR metabolism. In general, concentrations of 18 compounds, representing various classes of phytohormones, including brassinosteroids, auxins, cytokinins, gibberellins, abscisic acid, salicylic acid and jasmonic acid were analyzed under control and drought conditions in the “Bowman” cultivar and the BR-deficient NILs. Drought induced a significant increase in accumulation of the biologically active form of BRs—castasterone in all analyzed genotypes. Another biologically active form of BRs—24-epi-brassinolide—was identified in one, BR-insensitive NIL under normal condition, but its accumulation was drought-induced in all analyzed genotypes. Analysis of concentration profiles of several compounds representing gibberellins allowed an insight into the BR-dependent regulation of gibberellin biosynthesis. The concentration of the gibberellic acid GA7 was significantly lower in all NILs when compared with the “Bowman” cultivar, indicating that GA7 biosynthesis represents an enzymatic step at which the stimulating effect of BRs on gibberellin biosynthesis occurs. Moreover, the accumulation of GA7 is significantly induced by drought in all the genotypes. Biosynthesis of jasmonic acid is also a BR-dependent process, as all the NILs accumulated much lower concentrations of this hormone when compared with the “Bowman” cultivar under normal condition, however the accumulation of jasmonic acid, abscisic acid and salicylic acid were significantly stimulated by drought. © 2016 Gruszka, Janeczko, Dziurka, Pociecha, Oklestkova and Szarejko.},
note = {40},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Brassinosteroids (BRs) are a class of steroid phytohormones, which regulate various processes of morphogenesis and physiology—from seed development to regulation of flowering and senescence. An accumulating body of evidence indicates that BRs take part in regulation of physiological reactions to various stress conditions, including drought. Many of the physiological functions of BRs are regulated by a complicated, and not fully elucidated network of interactions with metabolic pathways of other phytohormones. Therefore, the aim of this study was to characterize phytohormonal homeostasis in barley (Hordeum vulgare) in reaction to drought and validate role of BRs in regulation of this process. Material of this study included the barley cultivar “Bowman” and five Near-Isogenic Lines (NILs) representing characterized semi-dwarf mutants of several genes encoding enzymes participating in BR biosynthesis and signaling. Analysis of endogenous BRs concentrations in these NILs confirmed that their phenotypes result from abnormalities in BR metabolism. In general, concentrations of 18 compounds, representing various classes of phytohormones, including brassinosteroids, auxins, cytokinins, gibberellins, abscisic acid, salicylic acid and jasmonic acid were analyzed under control and drought conditions in the “Bowman” cultivar and the BR-deficient NILs. Drought induced a significant increase in accumulation of the biologically active form of BRs—castasterone in all analyzed genotypes. Another biologically active form of BRs—24-epi-brassinolide—was identified in one, BR-insensitive NIL under normal condition, but its accumulation was drought-induced in all analyzed genotypes. Analysis of concentration profiles of several compounds representing gibberellins allowed an insight into the BR-dependent regulation of gibberellin biosynthesis. The concentration of the gibberellic acid GA7 was significantly lower in all NILs when compared with the “Bowman” cultivar, indicating that GA7 biosynthesis represents an enzymatic step at which the stimulating effect of BRs on gibberellin biosynthesis occurs. Moreover, the accumulation of GA7 is significantly induced by drought in all the genotypes. Biosynthesis of jasmonic acid is also a BR-dependent process, as all the NILs accumulated much lower concentrations of this hormone when compared with the “Bowman” cultivar under normal condition, however the accumulation of jasmonic acid, abscisic acid and salicylic acid were significantly stimulated by drought. © 2016 Gruszka, Janeczko, Dziurka, Pociecha, Oklestkova and Szarejko.
Janeczko, A.; Gruszka, D.; Pociecha, E.; Dziurka, M. A.; Filek, M.; Jurczyk, B.; Kalaji, H. M.; Kocurek, M.; Waligórski, P.
In: Plant Physiology and Biochemistry, vol. 99, pp. 126-141, 2016, ISSN: 09819428, (59).
@article{2-s2.0-84952690530,
title = {Physiological and biochemical characterisation of watered and drought-stressed barley mutants in the HvDWARF gene encoding C6-oxidase involved in brassinosteroid biosynthesis},
author = { A. Janeczko and D. Gruszka and E. Pociecha and M.A. Dziurka and M. Filek and B. Jurczyk and H.M. Kalaji and M. Kocurek and P. Waligórski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84952690530&doi=10.1016%2fj.plaphy.2015.12.003&partnerID=40&md5=5543838881428c116f22beb2fefc80e2},
doi = {10.1016/j.plaphy.2015.12.003},
issn = {09819428},
year = {2016},
date = {2016-01-01},
journal = {Plant Physiology and Biochemistry},
volume = {99},
pages = {126-141},
publisher = {Elsevier Masson SAS},
abstract = {Brassinosteroids (BR) are plant steroid hormones that were discovered more than thirty years ago, but their physiological function has yet to be fully explained. The aim of the study was to answer the question of whether/how disturbances in the production of BR in barley affects the plant's metabolism and development under conditions of optimal watering and drought. Mutants with an impaired production of BR are one of the best tools in research aimed at understanding the mechanisms of action of these hormones. The study used barley cultivars with a normal BR synthesis (wild type) and semi-dwarf allelic mutants with an impaired activity of C6-oxidase (mutation in HvDWARF), which resulted in a decreased BR synthesis. Half of the plants were subjected to drought stress in the seedling stage and the other half were watered optimally. Plants with impaired BR production were characterised by a lower height and developmental retardation. Under both optimal watering and drought, BR synthesis disorders caused the reduced production of ABA and cytokinins, but not auxins. The BR mutants also produced less osmoprotectant (proline). The optimally watered and drought-stressed mutants accumulated less sucrose, which was accompanied by changes in the production of other soluble sugars. The increased content of fructooligosaccharide (kestose) in optimally watered mutants would suggest that BR is a negative regulator of kestose production. The decreased level of nystose in the drought-stressed mutants also suggests BR involvement in the regulation of the production of this fructooligosaccharide. The accumulation of the transcripts of genes associated with stress response (hsp90) was lower in the watered and drought-stressed BR-deficient mutants. In turn, the lower efficiency of photosystem II and the net photosynthetic rate in mutants was revealed only under drought conditions. The presented research allows for the physiological and biochemical traits of two BR-barley mutants to be characterised, which helps BR function to be understood. The knowledge can also be a good starting point for some breeding companies that are interested in introducing new semi-dwarf barley cultivars. © 2015 Published by Elsevier Masson SAS.},
note = {59},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Brassinosteroids (BR) are plant steroid hormones that were discovered more than thirty years ago, but their physiological function has yet to be fully explained. The aim of the study was to answer the question of whether/how disturbances in the production of BR in barley affects the plant's metabolism and development under conditions of optimal watering and drought. Mutants with an impaired production of BR are one of the best tools in research aimed at understanding the mechanisms of action of these hormones. The study used barley cultivars with a normal BR synthesis (wild type) and semi-dwarf allelic mutants with an impaired activity of C6-oxidase (mutation in HvDWARF), which resulted in a decreased BR synthesis. Half of the plants were subjected to drought stress in the seedling stage and the other half were watered optimally. Plants with impaired BR production were characterised by a lower height and developmental retardation. Under both optimal watering and drought, BR synthesis disorders caused the reduced production of ABA and cytokinins, but not auxins. The BR mutants also produced less osmoprotectant (proline). The optimally watered and drought-stressed mutants accumulated less sucrose, which was accompanied by changes in the production of other soluble sugars. The increased content of fructooligosaccharide (kestose) in optimally watered mutants would suggest that BR is a negative regulator of kestose production. The decreased level of nystose in the drought-stressed mutants also suggests BR involvement in the regulation of the production of this fructooligosaccharide. The accumulation of the transcripts of genes associated with stress response (hsp90) was lower in the watered and drought-stressed BR-deficient mutants. In turn, the lower efficiency of photosystem II and the net photosynthetic rate in mutants was revealed only under drought conditions. The presented research allows for the physiological and biochemical traits of two BR-barley mutants to be characterised, which helps BR function to be understood. The knowledge can also be a good starting point for some breeding companies that are interested in introducing new semi-dwarf barley cultivars. © 2015 Published by Elsevier Masson SAS.