2019
Kolano, B. A.; McCann, J.; Oskędra, M.; Chrapek, M.; Rojek-Jelonek, M.; Nobis, A.; Weiss-Schneeweiss, H.
Parental origin and genome evolution of several eurasian hexaploid species of chenopodium (Chenopodiaceae) Journal Article
In: Phytotaxa, vol. 392, no. 3, pp. 163-185, 2019, ISSN: 11793155, (10).
@article{2-s2.0-85064201621,
title = {Parental origin and genome evolution of several eurasian hexaploid species of chenopodium (Chenopodiaceae)},
author = { B.A. Kolano and J. McCann and M. Oskędra and M. Chrapek and M. Rojek-Jelonek and A. Nobis and H. Weiss-Schneeweiss},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064201621&doi=10.11646%2fphytotaxa.392.3.1&partnerID=40&md5=6ba7b755e6a163ed3081a1dcbab2a333},
doi = {10.11646/phytotaxa.392.3.1},
issn = {11793155},
year = {2019},
date = {2019-01-01},
journal = {Phytotaxa},
volume = {392},
number = {3},
pages = {163-185},
publisher = {Magnolia Press},
abstract = {Hybridization and polyploidization appear to be widespread, or even ubiquitous, in the evolution of Chenopodium sensu stricto, but the origin and the evolutionary history of species of polyploid chenopods is still poorly understood. Phylogenetic analyses of DNA sequences of nrITS, four plastid regions, and the 5S rDNA spacer region (NTS) of five Eurasian hexaploid species (2n = 6x = 54), C. album, C. giganteum, C. pedunculare, C. formosanum, and C. opulifolium, and their diploid and tetraploid relatives, as well as genomic in situ hybridization (GISH), indicate their allohexaploid origin. It is inferred that the origins of all hexaploids analyzed here involved some B-genome diploid(s). The identity of the other parent/parents is more elusive. In the case of C. album, C. giganteum, and C. pedunculare, the second maternal parent seems to be similar to the C. betaceum (C. strictum auct.) or C. striatiforme, or Asian diploids. In genomes of allohexaploids C. album, C. giganteum, and C. pedunculare, half of their rDNA loci was located in chromosomes of the B-subgenome. The remaining rDNA loci were located in chromosomes that originated from another parent (or other parents). Although 35S rDNA loci inherited from two parental species seem to be present in these hexaploids, only one ribotype of nrITS was detected. © 2019 Magnolia Press.},
note = {10},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Hybridization and polyploidization appear to be widespread, or even ubiquitous, in the evolution of Chenopodium sensu stricto, but the origin and the evolutionary history of species of polyploid chenopods is still poorly understood. Phylogenetic analyses of DNA sequences of nrITS, four plastid regions, and the 5S rDNA spacer region (NTS) of five Eurasian hexaploid species (2n = 6x = 54), C. album, C. giganteum, C. pedunculare, C. formosanum, and C. opulifolium, and their diploid and tetraploid relatives, as well as genomic in situ hybridization (GISH), indicate their allohexaploid origin. It is inferred that the origins of all hexaploids analyzed here involved some B-genome diploid(s). The identity of the other parent/parents is more elusive. In the case of C. album, C. giganteum, and C. pedunculare, the second maternal parent seems to be similar to the C. betaceum (C. strictum auct.) or C. striatiforme, or Asian diploids. In genomes of allohexaploids C. album, C. giganteum, and C. pedunculare, half of their rDNA loci was located in chromosomes of the B-subgenome. The remaining rDNA loci were located in chromosomes that originated from another parent (or other parents). Although 35S rDNA loci inherited from two parental species seem to be present in these hexaploids, only one ribotype of nrITS was detected. © 2019 Magnolia Press.
2016
Kolano, B. A.; McCann, J.; Orzechowska, M.; Siwińska, D.; Temsch, E.; Weiss-Schneeweiss, H.
Molecular and cytogenetic evidence for an allotetraploid origin of Chenopodium quinoa and C. berlandieri (Amaranthaceae) Journal Article
In: Molecular Phylogenetics and Evolution, vol. 100, pp. 109-123, 2016, ISSN: 10557903, (32).
@article{2-s2.0-84962915550,
title = {Molecular and cytogenetic evidence for an allotetraploid origin of Chenopodium quinoa and C. berlandieri (Amaranthaceae)},
author = { B.A. Kolano and J. McCann and M. Orzechowska and D. Siwińska and E. Temsch and H. Weiss-Schneeweiss},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84962915550&doi=10.1016%2fj.ympev.2016.04.009&partnerID=40&md5=f32cb4019dbada940d643557f2d49c7e},
doi = {10.1016/j.ympev.2016.04.009},
issn = {10557903},
year = {2016},
date = {2016-01-01},
journal = {Molecular Phylogenetics and Evolution},
volume = {100},
pages = {109-123},
publisher = {Academic Press Inc.},
abstract = {Most of the cultivated chenopods are polyploids, but their origin and evolutionary history are still poorly understood. Phylogenetic analyses of DNA sequences of four plastid regions, nrITS and nuclear 5S rDNA spacer region (NTS) of two tetraploid chenopods (2n = 4x = 36), Andean C. quinoa and North American C. berlandieri, and their diploid relatives allowed inferences of their origin. The phylogenetic analyses confirmed allotetraploid origin of both tetraploids involving diploids of two different genomic groups (genomes A and B) and suggested that these two might share very similar parentage.The hypotheses on the origin of the two allopolyploid species were further tested using genomic in situ hybridization (GISH). Several diploid Chenopodium species belonging to the two lineages, genome A and B, suggested by phylogenetic analyses, were tested as putative parental taxa. GISH differentiated two sets of parental chromosomes in both tetraploids and further corroborated their allotetraploid origin. Putative diploid parental taxa have been suggested by GISH for C. quinoa and C. berlandieri. Genome sizes of the analyzed allotetraploids fit nearly perfectly the expected additive values of the putative parental taxa. Directional and uniparental loss of rDNA loci of the maternal A-subgenome was revealed for both C. berlandieri and C. quinoa. © 2016 Elsevier Inc.},
note = {32},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Most of the cultivated chenopods are polyploids, but their origin and evolutionary history are still poorly understood. Phylogenetic analyses of DNA sequences of four plastid regions, nrITS and nuclear 5S rDNA spacer region (NTS) of two tetraploid chenopods (2n = 4x = 36), Andean C. quinoa and North American C. berlandieri, and their diploid relatives allowed inferences of their origin. The phylogenetic analyses confirmed allotetraploid origin of both tetraploids involving diploids of two different genomic groups (genomes A and B) and suggested that these two might share very similar parentage.The hypotheses on the origin of the two allopolyploid species were further tested using genomic in situ hybridization (GISH). Several diploid Chenopodium species belonging to the two lineages, genome A and B, suggested by phylogenetic analyses, were tested as putative parental taxa. GISH differentiated two sets of parental chromosomes in both tetraploids and further corroborated their allotetraploid origin. Putative diploid parental taxa have been suggested by GISH for C. quinoa and C. berlandieri. Genome sizes of the analyzed allotetraploids fit nearly perfectly the expected additive values of the putative parental taxa. Directional and uniparental loss of rDNA loci of the maternal A-subgenome was revealed for both C. berlandieri and C. quinoa. © 2016 Elsevier Inc.
2015
Kolano, B. A.; Siwińska, D.; McCann, J.; Weiss-Schneeweiss, H.
The evolution of genome size and rDNA in diploid species of Chenopodium s.l. (Amaranthaceae) Journal Article
In: Botanical Journal of the Linnean Society, vol. 179, no. 2, pp. 218-235, 2015, ISSN: 00244074, (20).
@article{2-s2.0-84941316011,
title = {The evolution of genome size and rDNA in diploid species of Chenopodium s.l. (Amaranthaceae)},
author = { B.A. Kolano and D. Siwińska and J. McCann and H. Weiss-Schneeweiss},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84941316011&doi=10.1111%2fboj.12321&partnerID=40&md5=e7855ebbd9a0efc76233b689ff8c7d98},
doi = {10.1111/boj.12321},
issn = {00244074},
year = {2015},
date = {2015-01-01},
journal = {Botanical Journal of the Linnean Society},
volume = {179},
number = {2},
pages = {218-235},
publisher = {Blackwell Publishing Ltd},
abstract = {The evolution of genome size and ribosomal DNA (rDNA) locus organization was analysed in 23 diploid species of Chenopodium s.l., all of which share the same base chromosome number of x=9. Phylogenetic relationships among these species were inferred from plastid and nuclear ribosomal internal transcribed spacer (nrITS) DNA sequences. The molecular phylogenetic analyses assigned all analysed species of Chenopodium s.l. to six evolutionary lineages, corresponding to the recent new generic taxonomic treatment of Chenopodium s.l. The distribution of rDNA loci for four species is presented here for the first time using fluorescence insitu hybridization (FISH) with 5S and 35S rDNA probes. Most of the 23 analysed diploid Chenopodium spp. possessed a single subterminally located 35S rDNA locus, except for three species which possessed two 35S rDNA loci. One or two 5S rDNA loci were typically localized subterminally on chromosomes, rarely interstitially. Analyses of rDNA locus numbers in a phylogenetic context resulted in the reconstruction of one locus each of 35S rDNA and 5S rDNA, both in subterminal positions, as the ancestral state. Genome sizes determined using flow cytometry were relatively small (2C value<2.8pg), ranging from 0.734pg in C.schraderianum to 2.721pg in C.californicum (nearly four-fold difference), and were often conserved within major phylogenetic lineages, suggesting an adaptive value. The reconstructed ancestral genome size was small for all evolutionary lineages, and changes have probably coincided with the divergence of major lineages. © 2015 The Linnean Society of London.},
note = {20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The evolution of genome size and ribosomal DNA (rDNA) locus organization was analysed in 23 diploid species of Chenopodium s.l., all of which share the same base chromosome number of x=9. Phylogenetic relationships among these species were inferred from plastid and nuclear ribosomal internal transcribed spacer (nrITS) DNA sequences. The molecular phylogenetic analyses assigned all analysed species of Chenopodium s.l. to six evolutionary lineages, corresponding to the recent new generic taxonomic treatment of Chenopodium s.l. The distribution of rDNA loci for four species is presented here for the first time using fluorescence insitu hybridization (FISH) with 5S and 35S rDNA probes. Most of the 23 analysed diploid Chenopodium spp. possessed a single subterminally located 35S rDNA locus, except for three species which possessed two 35S rDNA loci. One or two 5S rDNA loci were typically localized subterminally on chromosomes, rarely interstitially. Analyses of rDNA locus numbers in a phylogenetic context resulted in the reconstruction of one locus each of 35S rDNA and 5S rDNA, both in subterminal positions, as the ancestral state. Genome sizes determined using flow cytometry were relatively small (2C value<2.8pg), ranging from 0.734pg in C.schraderianum to 2.721pg in C.californicum (nearly four-fold difference), and were often conserved within major phylogenetic lineages, suggesting an adaptive value. The reconstructed ancestral genome size was small for all evolutionary lineages, and changes have probably coincided with the divergence of major lineages. © 2015 The Linnean Society of London.