@article{2-s2.0-85153846939,

title = {Multifractal Analysis of the Influence of Indole-3-Acetic Acid on Fast-Activating Vacuolar (FV) Channels of Beta vulgaris L. Taproot Cells},

author = { J. Miśkiewicz and Z. Burdach and Z. Trela and A. Siemieniuk and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85153846939&doi=10.3390%2fmembranes13040406&partnerID=40&md5=82d64bccdd33b677f6cce9e8792098fe},

doi = {10.3390/membranes13040406},

issn = {20770375},

year  = {2023},

date = {2023-01-01},

journal = {Membranes},

volume = {13},

number = {4},

publisher = {MDPI},

abstract = {In this paper, the multifractal properties of the ion current time series in the fast-activating vacuolar (FV) channels of Beta vulgaris L. taproot cells were investigated. These channels are permeable for only monovalent cations and mediate K+ at very low concentrations of cytosolic Ca2+ and large voltages of either polarity. Using the patch clamp technique, the currents of the FV channels in red beet taproot vacuoles were recorded and analysed by using the multifractal detrended fluctuation analysis (MFDFA) method. The activity of the FV channels depended on the external potential and was sensitive to the auxin. It was also shown that the singularity spectrum of the ion current in the FV channels is non-singular, and the multifractal parameters, i.e., the generalised Hurst exponent and the singularity spectrum, were modified in the presence of IAA. Taking into account the obtained results, it can be suggested that the multifractal properties of fast-activating vacuolar (FV) K+ channels, indicating the existence of long-term memory, should be taken into account in the molecular mechanism of the auxin-induced growth of plant cells. © 2023 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85149128761,

title = {The Effects of 1,4-Naphthoquinone (NQ) and Naphthazarin (5,8-Dihydroxy-1,4-naphthoquinone, DHNQ) Individually and in Combination on Growth and Oxidative Stress in Maize (Zea mays L.) Seedlings},

author = { W. Karcz and Z. Burdach and M. Rudnicka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149128761&doi=10.3390%2fplants12040900&partnerID=40&md5=ad6c9862904a9ec0e860c0b5e4293ac1},

doi = {10.3390/plants12040900},

issn = {22237747},

year  = {2023},

date = {2023-01-01},

journal = {Plants},

volume = {12},

number = {4},

publisher = {MDPI},

abstract = {This study investigated the effects of 1,4-naphthoquinone (NQ) and naphthazarin (5;8-dihydroxy-1;4-naphthoquinone; DHNQ) individually and in combination, applied at low concentrations (0.1; 1; and 10 nM), on growth, hydrogen peroxide (H2O2) production, catalase activity, and lipid peroxidation in maize seedlings. It was found that NQ at 0.1 and 1 nM and DHNQ at 0.1 nM significantly stimulated the fresh weight of the aboveground parts of the seedlings (APS), while the fresh weight of the underground parts of the seedlings (UPS) was enhanced only at 0.1 nM NQ. Interestingly, DHNQ at higher concentrations (1 and 10 nM) significantly diminished the fresh weight of the APS and UPS. When NQ and DHNQ were applied together, an increase in the fresh weight of the APS at all of the concentrations studied was observed. It was also found that NQ and DHNQ individually and in combination, at all concentrations studied, decreased the H2O2 production in the aboveground and underground parts of maize seedlings. The presence of the DHNQ at higher concentrations (1 and 10 nM) triggered an increase in the catalase (CAT) activity of the UPS and APS compared to the control. However, NQ added at 1 nM decreased the CAT activity of both the UPS and APS, while 10 nM increased the CAT activity of UPS. NQ and DHNQ applied together at 0.1 and 10 nM almost completely inhibited catalase activity in the UPS and APS. The data that were obtained for lipid peroxidation, measured as the malondialdehyde (MDA) concentration, indicated that NQ and DHNQ at all concentrations studied decreased the MDA content of the UPS, while both naphthoquinones increased it in APS. The data presented here are discussed taking into account the mechanisms via which naphthoquinones interact with biological systems. © 2023 by the authors.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85135264238,

title = {The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study},

author = { W. Karcz and Z. Burdach},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135264238&doi=10.1186%2fs12870-022-03778-4&partnerID=40&md5=9237dfe8d894a1e13df9f6fe76439d1f},

doi = {10.1186/s12870-022-03778-4},

issn = {14712229},

year  = {2022},

date = {2022-01-01},

journal = {BMC Plant Biology},

volume = {22},

number = {1},

publisher = {BioMed Central Ltd},

abstract = {Background: In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results: It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root; mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA; indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion: It is suggested that a DC electric field inhibits the plasma membrane H+ pump activity and K+ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays K+ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. © 2022, The Author(s).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85119610574,

title = {A comparison of the effect of lead (Pb) on the slow vacuolar (sv) and fast vacuolar (fv) channels in red beet (beta vulgaris l.) taproot vacuoles},

author = { A. Siemieniuk and Z. Burdach and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119610574&doi=10.3390%2fijms222312621&partnerID=40&md5=b9f8b810e216555eaed034f340681a77},

doi = {10.3390/ijms222312621},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {23},

publisher = {MDPI},

abstract = {Little is known about the effect of lead on the activity of the vacuolar K+ channels. Here, the patch-clamp technique was used to compare the impact of lead (PbCl2) on the slow-activating (SV) and fast-activating (FV) vacuolar channels. It was revealed that, under symmetrical 100-mM K+, the macroscopic currents of the SV channels exhibited a typical slow activation and a strong outward rectification of the steady-state currents, while the macroscopic currents of the FV channels displayed instantaneous currents, which, at the positive potentials, were about three-fold greater compared to the one at the negative potentials. When PbCl2 was added to the bath solution at a final concentration of 100 µM, it decreased the macroscopic outward currents of both channels but did not change the inward currents. The single-channel recordings demonstrated that cytosolic lead causes this macroscopic effect by a decrease of the single-channel conductance and decreases the channel open probability. We propose that cytosolic lead reduces the current flowing through the SV and FV channels, which causes a decrease of the K+ fluxes from the cytosol to the vacuole. This finding may, at least in part, explain the mechanism by which cytosolic Pb2+ reduces the growth of plant cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85118695038,

title = {Expansion Time of the Thermal Multifragmentation in the Interaction of 22 GeV Carbon Nuclei with a Gold Target},

author = { S.P. Avdeyev and W. Karcz and V.V. Kirakosyan and P.A. Rukoyatkin and V.I. Stegaylov and A.S. Botvina},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118695038&doi=10.3103%2fS1062873821100063&partnerID=40&md5=ba0f2b711a133454b0ac56e50fd237d3},

doi = {10.3103/S1062873821100063},

issn = {10628738},

year  = {2021},

date = {2021-01-01},

journal = {Bulletin of the Russian Academy of Sciences: Physics},

volume = {85},

number = {10},

pages = {1077-1079},

publisher = {Pleiades journals},

abstract = {Abstract: The relative angle correlations of intermediate mass fragments has been studied for 12C + Au collisions at 22 GeV. Strong suppression at small angles is observed which is due to the Coulomb repulsion of fragments. The experimental correlation function was compared to that obtained by the multibody Coulomb trajectory calculations with the various decay time of fragmenting system. It is found that the average decay time of fragmenting system is less than 59 ± 11 fm c–1. © 2021, Allerton Press, Inc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85105393802,

title = {Fusicoccin (Fc)-induced rapid growth, proton extrusion and membrane potential changes in maize (zea mays l.) coleoptile cells: Comparison to auxin responses},

author = { M. Polak and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105393802&doi=10.3390%2fijms22095017&partnerID=40&md5=49336a5ad3dce6b1b5dbdd8c1c98f766},

doi = {10.3390/ijms22095017},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {9},

publisher = {MDPI AG},

abstract = {The fungal toxin fusicoccin (FC) induces rapid cell elongation, proton extrusion and plasma membrane hyperpolarization in maize coleoptile cells. Here, these three parameters were simultaneously measured using non-abraded and non-peeled segments with the incubation medium having access to their lumen. The dose–response curve for the FC-induced growth was sigmoidal shaped with the maximum at 10−6 M over 10 h. The amplitudes of the rapid growth and proton extrusion were significantly higher for FC than those for indole-3-acetic acid (IAA). The differences between the membrane potential changes that were observed in the presence of FC and IAA relate to the permanent membrane hyperpolarization for FC and transient hyperpolarization for IAA. It was also found that the lag times of the rapid growth, proton extrusion and membrane hyperpolarization were shorter for FC compared to IAA. At 30◦C, the biphasic kinetics of the IAA-induced growth rate could be changed into a monophasic (parabolic) one, which is characteristic for FC-induced rapid growth. It has been suggested that the rates of the initial phase of the FC-and IAA-induced growth involve two common mechanisms that consist of the proton pumps and potassium channels whose contribution to the action of both effectors on the rapid growth is different. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85101557306,

title = {Some new methodological and conceptual aspects of the “acid growth theory” for the auxin action in maize (Zea mays l.) coleoptile segments: Do acid-and auxin-induced rapid growth differ in their mechanisms?},

author = { M. Polak and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101557306&doi=10.3390%2fijms22052317&partnerID=40&md5=1c4334f99282466371393805df07a041},

doi = {10.3390/ijms22052317},

issn = {16616596},

year  = {2021},

date = {2021-01-01},

journal = {International Journal of Molecular Sciences},

volume = {22},

number = {5},

pages = {1-17},

publisher = {MDPI AG},

abstract = {Two arguments against the “acid growth theory” of auxin-induced growth were re-examined. First, the lack of a correlation between the IAA-induced growth and medium acidification, which is mainly due to the cuticle, which is a barrier for proton diffusion. Second, acid-and the IAA-induced growth are additive processes, which means that acid and the IAA act via different mechanisms. Here, growth, medium pH, and membrane potential (in some experiments) were simultaneously measured using non-abraded and non-peeled segments but with the incubation medium having access to their lumen. Using such an approach significantly enhances both the IAAinduced growth and proton extrusion (similar to that of abraded segments). Staining the cuticle on the outer and inner epidermis of the coleoptile segments showed that the cuticle architecture differs on both sides of the segments. The dose-response curves for the IAA-induced growth and proton extrusion were bell-shaped with the maximum at 10−4 M over 10 h. The kinetics of the IAA-induced hyperpolarisation was similar to that of the rapid phase of the IAA-induced growth. It is also proposed that the K+/H+ co-transporters are involved in acid-induced growth and that the combined effect of the K+ channels and K+/ H+ co-transporters is responsible for the IAA-induced growth. These findings support the “acid growth theory” of auxin action. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85091093435,

title = {Source Velocity in Collisions between 3.6 GeV Protons and a Gold Target},

author = { S.P. Avdeyev and W. Karcz and V.V. Kirakosyan and P.A. Rukoyatkin and V.I. Stegaylov and A.S. Botvina},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091093435&doi=10.3103%2fS1062873820080080&partnerID=40&md5=995942dca151619daf3868c9ef2bb01f},

doi = {10.3103/S1062873820080080},

issn = {10628738},

year  = {2020},

date = {2020-01-01},

journal = {Bulletin of the Russian Academy of Sciences: Physics},

volume = {84},

number = {8},

pages = {979-980},

publisher = {Pleiades journals},

abstract = {Abstract: Invariant cross sections of carbon fragments are investigated for p(3.6 GeV) + Au collisions in terms of longitudinal versus transversal velocity components. It is found that fragments are emitted by one slowly moving source. © 2020, Allerton Press, Inc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85087646572,

title = {Effect of auxin (IAA) on the fast vacuolar (FV) channels in red beet (Beta vulgaris L.) taproot vacuoles},

author = { Z. Burdach and A. Siemieniuk and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087646572&doi=10.3390%2fijms21144876&partnerID=40&md5=3d4cb4c0970cadbc3552dc5cac22e835},

doi = {10.3390/ijms21144876},

issn = {16616596},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Molecular Sciences},

volume = {21},

number = {14},

pages = {1-11},

publisher = {MDPI AG},

abstract = {In contrast to the well-studied effect of auxin on the plasma membrane K+ channel activity, little is known about the role of this hormone in regulating the vacuolar K+ channels. Here, the patch-clamp technique was used to investigate the effect of auxin (IAA) on the fast-activating vacuolar (FV) channels. It was found that the macroscopic currents displayed instantaneous currents, which at the positive potentials were about three-fold greater compared to the one at the negative potentials. When auxin was added to the bath solution at a final concentration of 1 µM, it increased the outward currents by about 60%, but did not change the inward currents. The imposition of a ten-fold vacuole-to-cytosol KCl gradient stimulated the efflux of K+ from the vacuole into the cytosol and reduced the K+ current in the opposite direction. The addition of IAA to the bath solution with the 10/100 KCl gradient decreased the outward current and increased the inward current. Luminal auxin reduced both the outward and inward current by approximately 25% compared to the control. The single channel recordings demonstrated that cytosolic auxin changed the open probability of the FV channels at the positive voltages to a moderate extent, while it significantly increased the amplitudes of the single channel outward currents and the number of open channels. At the positive voltages, auxin did not change the unitary conductance of the single channels. We suggest that auxin regulates the activity of the fast-activating vacuolar (FV) channels, thereby causing changes of the K+ fluxes across the vacuolar membrane. This mechanism might serve to tightly adjust the volume of the vacuole during plant cell expansion. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85080900098,

title = {Long range correlations of the ion current in SV channels. Met3PbCl influence study},

author = { J. Miśkiewicz and Z. Trela and Z. Burdach and W. Karcz and W. Balińska-Miśkiewicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85080900098&doi=10.1371%2fjournal.pone.0229433&partnerID=40&md5=10fddf27191100d7c31abf9471d441d0},

doi = {10.1371/journal.pone.0229433},

issn = {19326203},

year  = {2020},

date = {2020-01-01},

journal = {PLoS ONE},

volume = {15},

number = {3},

publisher = {Public Library of Science},

abstract = {The long-range correlations within the current signal time series of the Beta vulgaris vacuolar membrane under the influence of organolead compound (Met3PbCl) are investigated. The current time series is transformed into a dwell time series. Then the rescaled range and detrended fluctuations analyses are used. It is shown that the presence of Met3PbCl in the solution decreases the mean value of the Hurst exponent and therefore influences the longrange correlations in ionic channel current. This observation is statistically significant. An ion channel model is built and the experimental results reconstructed and analysed. © 2020 Miśkiewicz et al.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85066796904,

title = {Radial Flow in the Interaction of Relativistic Deuterons with a Gold Target},

author = { S.P. Avdeyev and W. Karcz and V.V. Kirakosyan and P.A. Rukoyatkin and V.I. Stegaylov and A.S. Botvina},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066796904&doi=10.3103%2fS106287381904004X&partnerID=40&md5=66152cd033b4778636d1f6170a82cd0f},

doi = {10.3103/S106287381904004X},

issn = {10628738},

year  = {2019},

date = {2019-01-01},

journal = {Bulletin of the Russian Academy of Sciences: Physics},

volume = {83},

number = {4},

pages = {399-401},

publisher = {Pleiades Publishing},

abstract = {Abstract: The kinetic energy spectra of intermediate mass fragments (IMF) has been studied for 4.4 GeV d + Au collisions at the Dubna Nuclotron with the 4π detector array FASA. Experimental kinetic energy spectra were compared to that obtained by the multi-body Coulomb trajectory calculations with the initial break-up conditions given by the combined model INC + SMM. It was found good agreement of measured and calculated kinetic energy spectra including a radial flow. © 2019, Allerton Press, Inc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85064822948,

title = {Effects of naphthazarin (DHNQ) combined with lawsone (NQ-2-OH) or 1,4-naphthoquinone (NQ) on the auxin-induced growth of zea mays L. coleoptile segments},

author = { M. Rudnicka and M. Ludynia and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064822948&doi=10.3390%2fijms20071788&partnerID=40&md5=519ba84ec7918f2374c28a97c20e6518},

doi = {10.3390/ijms20071788},

issn = {16616596},

year  = {2019},

date = {2019-01-01},

journal = {International Journal of Molecular Sciences},

volume = {20},

number = {7},

publisher = {MDPI AG},

abstract = {Naphthoquinones, plants secondary metabolites are known for their antibacterial, antifungal, anti-inflammatory, anti-cancer and anti-parasitic properties. The biological activity of naphthoquinones is connected with their ability to generate reactive oxygen species and to modify biological molecules at their nucleophilic sites. In our research, the effect of naphthazarin (DHNQ) combined with 2-hydroxy-1,4-naphthoquinone (NQ-2-OH) or 1,4-naphthoquinone (1;4-NQ) on the elongation growth, pH changes of the incubation medium, oxidative stress and redox activity of maize coleoptile cells were investigated. This paper describes experiments performed with maize (Zea mays L.) coleoptile segments, which is a classical model system to study plant cell elongation growth. The data presented clearly demonstrate that lawsone and 1,4-naphthoquinone combined with naphthazarin, at low concentrations (1 and 10 nM), reduced the endogenous and IAA-induced (Indole-3-Acetic Acid) elongation growth of maize coleoptile segments. Those changes in growth correlated with the proton concentration in the incubation medium, which suggests that the changes in the growth of maize coleoptile segments observed in the presence of naphthoquinones are mediated through the activity of PM H+-ATPase. The presence of naphthoquinones induced oxidative stress in the maize coleoptile tissue by producing hydrogen peroxide and causing changes in the redox activity. Moreover, the incubation of maize segments with both naphthoquinones combined with naphthazarin resulted in lipid peroxidation and membrane damage. The regulation of PM H+-ATPase activity, especially its inhibition, may result from two major types of reaction: first, a direct interaction between an enzyme and naphthoquinone, which leads to the covalent modification of the protein thiols and the generation of thioethers, which have been found to alter the activity of the PM H+-ATPases; second, naphthoquinones induce reactive oxygen species (ROS) production, which inhibits PM H+-ATPases by increasing cytosolic Ca2+ . This harmful effect was stronger when naphthazarin and 1,4-naphthoquinone were added together. Taking these results into account, it can be suggested that by combining naphthoquinones in small quantities, an alternative to synthetic pesticides could be developed. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85062730921,

title = {The effect of naphthazarin on the growth, electrogenicity, oxidative stress, and microtubule array in Z. Mays coleoptile cells treated with IAA},

author = { M. Rudnicka and M. Ludynia and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062730921&doi=10.3389%2ffpls.2018.01940&partnerID=40&md5=152e6cc69e812dbcc06e9ae50a10b1fb},

doi = {10.3389/fpls.2018.01940},

issn = {1664462X},

year  = {2019},

date = {2019-01-01},

journal = {Frontiers in Plant Science},

volume = {9},

publisher = {Frontiers Media S.A.},

abstract = {Naphthazarin (5;8-dihydroxy-1;4-naphthoquinone; DHNQ) is a naturally occurring 1,4-naphthoquinone derivative. In this study, we focused on elucidating the toxic effect of this secondary metabolite on the growth of plant cells. The dose–response curves that were obtained for the effects of DHNQ on endogenous and IAA-induced growth in maize coleoptile segments differ in shape; in the first case, it is linear, while in the presence of auxin it is bell-shaped with the maximum at 1 µM. It was found that DHNQ at almost all concentrations studied, when added to the incubation medium inhibited endogenous growth (excluding naphthazarin at 0.001 µM) as well as growth in the presence of IAA. Simultaneous measurements of the growth and external medium pH of coleoptile segments indicated that DHNQ diminished or eliminated proton extrusion at all of the concentrations that were used. Interestingly, the oxidative stress in maize coleoptile cells, which was measured as hydrogen peroxide (H 2 O 2 ) production, catalase activity, redox activity and malondialdehyde (MDA) content, increased at the lower concentrations of DHNQ (<1 µM), thus suggesting a specific character of its action. It was also found that naphthazarin at concentration higher than 0.1 µM caused the depolarization of the membrane potential (E m ). An analysis of the organization and anisotropy of the cortical microtubules showed that naphthazarin at all of the concentrations that were studied changed the IAA-induced transverse microtubule reorientation to an oblique reorientation. Our results indicate that naphthazarin diminished the growth of maize coleoptile cells by a broad spectrum of its toxic effects, thereby suggesting that naphthazarin might be a hypothetical component of new bioherbicides and biopesticides. © 2019 Rudnicka, Ludynia and Karcz.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85047635603,

title = {Single and combined effects of Cd and Pb on the growth, medium pH, membrane potential and metal contents in maize (Zea mays L.) coleoptile segments},

author = { R. Kurtyka and Z. Burdach and A. Siemieniuk and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047635603&doi=10.1016%2fj.ecoenv.2018.05.046&partnerID=40&md5=18ad1f9d7d8a7c274468b9a3fbb3820b},

doi = {10.1016/j.ecoenv.2018.05.046},

issn = {01476513},

year  = {2018},

date = {2018-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {161},

pages = {8-16},

publisher = {Academic Press},

abstract = {The mechanisms of the toxic effects of Cd and Pb on plant cell growth are still poorly understood. In particular, little is known about their interactive effects, which usually occur in the environment. Moreover, the data that do exist in the literature are controversial. This study describes experiments that were performed with maize (Zea mays) coleoptile segments, which is a classical model system for studies of plant cell elongation growth. Cadmium and lead, which were added at 0.1 mM, reduced the endogenous and IAA-induced elongation growth of maize coleoptile cells. When both metals were added together or in sequence, their effect on IAA-induced growth was more toxic. The medium pH changes, which were measured simultaneously with growth, indicated that while Pb stopped IAA-induced proton extrusion, Cd only partially diminished it. Although Cd was generally more accumulated than Pb in the maize coleoptile segments, when IAA was added together with Pb, it significantly increased the accumulation of the metal. The short-term electrophysiological experiments showed that the addition of Cd caused the depolarisation of the membrane potential (E m ), whereas Pb caused membrane hyperpolarisation. In the long-term electrophysiological experiments, it was found that the Cd-induced E m changes are complex. In conclusion, these results suggest that the effects of Cd and Pb as well as their combination on the elongation growth of maize coleoptile cells and the accumulation of the metals result, among others, from different ionic mechanisms by which each metal change the membrane potential of the cells. © 2018 Elsevier Inc.},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85048040874,

title = {Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles},

author = { Z. Burdach and A. Siemieniuk and Z. Trela and R. Kurtyka and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048040874&doi=10.1186%2fs12870-018-1321-6&partnerID=40&md5=8db44cbfb0dd470943970e766f250d99},

doi = {10.1186/s12870-018-1321-6},

issn = {14712229},

year  = {2018},

date = {2018-01-01},

journal = {BMC Plant Biology},

volume = {18},

number = {1},

publisher = {BioMed Central Ltd.},

abstract = {Background: Auxin (IAA) is a central player in plant cell growth. In contrast to the well-established function of the plasma membrane in plant cell expansion, little is known about the role of the vacuolar membrane (tonoplast) in this process. Results: It was found that under symmetrical 100 mM K+ and 100 μM cytoplasmic Ca2+ the macroscopic currents showed a typical slow activation and a strong outward rectification of the steady-state currents. The addition of IAA at a final concentration of 1 μM to the bath medium stimulated the SV currents, whereas at 0.1 and 10 μM slight inhibition of SV currents was observed. The time constant, decreased in the presence of this hormone. When single channels were analyzed, an increase in their activity was recorded with IAA compared to the control. The single-channel recordings that were obtained in the presence of IAA showed that auxin increased the amplitude of the single-channel currents. Interestingly, the addition of IAA to the bath medium with the same composition as the one that was used in the patch-clamp experiments showed that auxin decreased the volume of the vacuoles. Conclusions: It is suggested that the SV channels and the volume of red beet taproot vacuoles are modulated by auxin (IAA). © 2018 The Author(s).},

note = {8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85029784691,

title = {A comparison of the effects of 1,4-naphthoquinone and 2-hydroxy-1,4-naphthoquinone (lawsone) on indole-3-acetic acid (IAA)-induced growth of maize coleoptile cells},

author = { M. Rudnicka and M. Ludynia and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029784691&doi=10.1007%2fs10725-017-0325-9&partnerID=40&md5=ef2c82714d7abab030552a7db9f7ed59},

doi = {10.1007/s10725-017-0325-9},

issn = {01676903},

year  = {2018},

date = {2018-01-01},

journal = {Plant Growth Regulation},

volume = {84},

number = {1},

pages = {107-122},

publisher = {Springer Netherlands},

abstract = {The effects of 1,4-naphthoquinone (NQ) and 2-hydroxy-1,4-naphthoquinone (NQ-2-OH) on indole-3-acetic acid (IAA)-induced growth, medium pH changes and membrane potential (Em) in maize (Zea mays L.) coleoptile cells were determined. In addition, the redox cycling properties of both naphthoquinones were also compared. The dose-response curves constructed for the effects of NQ and NQ-2-OH on endogenous and IAA-induced growth differ in shape. It was found that NQ was by 10–50% more effective in inhibiting IAA-induced growth in maize coleoptile segments than NQ-2-OH. Simultaneous measurements of growth and external medium pH indicated that NQ and NQ-2-OH reduced or eliminated proton extrusion at all of the concentrations used, excluding NQ at 1 µM. It was found that both naphthoquinones at concentrations higher than 10 µM caused the depolarisation of the membrane potential (Em). Additionally, compared to the controls, NQ- and NQ-2-OH-exposure of coleoptile segments, at concentrations higher than 10 µM, caused an elevation of the hydrogen peroxide (H2O2) production and plasma membrane redox activity. The highest catalase activity was observed at 10 µM NQ and it was ca. 18-fold greater (at 4 h) than in the control medium. Moreover, it was also found that NQ and NQ-2-OH, at all concentrations studied, increased the malondialdehyde content of coleoptile segments at 4 h of the experiment. The data presented here are discussed taking into account the “acid growth hypothesis” of auxin action and the mechanisms by which naphthoquinones interact with biological systems. © 2017, The Author(s).},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84968909111,

title = {Electrical properties of as-grown and proton-irradiated high purity silicon},

author = { J. Krupka and W. Karcz and P. Kamiński and L. Jensen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84968909111&doi=10.1016%2fj.nimb.2016.05.007&partnerID=40&md5=0dd9884645c43894dc46cea4cdefac45},

doi = {10.1016/j.nimb.2016.05.007},

issn = {0168583X},

year  = {2016},

date = {2016-01-01},

journal = {Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms},

volume = {380},

pages = {76-83},

publisher = {Elsevier B.V.},

abstract = {The complex permittivity of as-grown and proton-irradiated samples of high purity silicon obtained by the floating zone method was measured as a function of temperature at a few frequencies in microwave spectrum by employing the quasi TE011 and whispering gallery modes excited in the samples under test. The resistivity of the samples was determined from the measured imaginary part of the permittivity. The resistivity was additionally measured at RF frequencies employing capacitive spectroscopy as well as in a standard direct current experiment. The sample of as-grown material had the resistivity of ∼85 kΩ cm at room temperature. The sample irradiated with 23-MeV protons had the resistivity of ∼500 kΩ cm at 295 K and its behavior was typical of the intrinsic material at room and at elevated temperatures. For the irradiated sample, the extrinsic conductivity region is missing and at temperatures below 250 K hopping conductivity occurs. Thermal cycle hysteresis of the resistivity for the sample of as-grown material is observed. After heating and subsequent cooling of the sample, its resistivity decreases and then slowly (∼50 h) returns to the initial value. © 2016 Elsevier B.V. All rights reserved.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84971327867,

title = {Source velocity at relativistic beams of 4He},

author = { S.P. Avdeyev and V.A. Karnaukhov and W. Karcz and V.V. Kirakosyan and P.A. Rukoyatkin and V.I. Stegaylov and H. Oeschler and A.S. Botvina},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84971327867&doi=10.3103%2fS1062873816030047&partnerID=40&md5=67d24174f9fe404615ce4c3bb28e7bb8},

doi = {10.3103/S1062873816030047},

issn = {10628738},

year  = {2016},

date = {2016-01-01},

journal = {Bulletin of the Russian Academy of Sciences: Physics},

volume = {80},

number = {3},

pages = {330-332},

publisher = {Allerton Press Incorporation},

abstract = {The source velocities (β = ν/c) extracted from rapidity plots of the fragment invariant probability distribution in terms of the longitudinal versus transversal velocity components has been studied for 4He + Au collisions at 4 and 14.6 GeV. It was found transition from broad range source velocities distribution in case of 4He(4 GeV) + Au to fixed source velocity in case of 4He(14.6 GeV) + Au. © 2016, Allerton Press, Inc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85019563519,

title = {Effect of low frequency pulsed magnetic field on gravitropic response & cell elongation in coleoptiles of maize seedlings},

author = { A. Kościarz-Grzesiok and K. Sieroń-Stołtny and M. Polak and A. Sieroń and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019563519&doi=10.4149%2fgpb_2016010&partnerID=40&md5=6fbc94be4b8918fefa64e13031548b99},

doi = {10.4149/gpb_2016010},

issn = {02315882},

year  = {2016},

date = {2016-01-01},

journal = {General Physiology and Biophysics},

volume = {35},

number = {4},

pages = {417-424},

publisher = {Slovak Academy of Sciences},

abstract = {The effect of pulsed magnetic field (PMF) on gravitropic response, endogenous growth and growth in the presence of indole-3-acetic acid (IAA) was studied in coleoptiles of maize (Zea mays L.) seedlings. Medium pH changes, measured simultaneously with growth of coleoptile segments, were also determined. In seedlings grown in the presence of PMF, elongation growth of coleoptiles was inhibited by 16%, while growth of roots and mesocotyls did not depend on PMF. Magnetic field also inhibited (by 36%) the gravitropic response of maize seedlings. However, when PMF was applied only during gravistimulation (within 6 h), it suppressed the gravitropic reaction only by 8% at 6 h. It was also found that endogenous growth and IAA-induced growth of maize coleoptile segments excised from seedlings treated with the PMF was stimulated by 52% and 30%, respectively, as compared to control (segments untreated with the PMF). Values of medium pH, measured simultaneously with growth, indicated that PMF-treated coleoptile segments extruded much more protons than untreated segments. In contrast, coleoptile segments treated with the PMF and subsequently incubated in the presence of IAA extruded 2.5-fold less protons as compared to segments treated with IAA only. The data presented here have been discussed with consideration of mechanisms by which auxin (IAA) regulates plant cell growth.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85016081207,

title = {Effects of juglone and lawsone on oxidative stress in maize coleoptile cells treated with IAA},

author = { R. Kurtyka and W. Pokora and Z. Tukaj and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016081207&doi=10.1093%2faobpla%2fplw073&partnerID=40&md5=5c587fc547e25b1e15221aea5c214717},

doi = {10.1093/aobpla/plw073},

issn = {20412851},

year  = {2016},

date = {2016-01-01},

journal = {AoB PLANTS},

volume = {8},

publisher = {Oxford University Press},

abstract = {Naphthoquinones are secondary metabolites widely distributed in nature and produced by bacteria, fungi and higher plants. Their biological activity may result from induction of oxidative stress, caused by redox cycling or direct interaction with cellular macromolecules, in which quinones act as electrophiles. The redox homeostasis is known as one of factors involved in auxin-mediated plant growth regulation. To date, however, little is known about the crosstalk between reactive oxygen species (ROS) produced by quinones and the plant growth hormone auxin (IAA). In this study, redox cycling properties of two naphthoquinones, juglone (5-hydroxy-1;4-naphthoquinone) and lawsone (2-hydroxy-1;4-naphthoquinone), were compared in experiments performed on maize coleoptile segments incubated with or without the addition of IAA. It was found that lawsone was much more effective than juglone in increasing both H2O2 production and the activity of antioxidative enzymes (SOD; POX and CAT) in coleoptile cells, regardless of the presence of IAA. An increase in the activity of Cu/Zn-SOD isoenzymes induced by both naphthoquinones suggests that juglone- and lawsone-generated H2O2 was primarily produced in the cytosolic and cell wall spaces. The cell potential to neutralize hydrogen peroxide, determined by POX and CAT activity, pointed to activity of catalase as the main enzymatic mechanism responsible for degradation of H2O2. Therefore, we assumed that generation of H2O2, induced more efficiently by LW than JG, was the major factor accounting for differences in the toxicity of naphthoquinones in maize coleoptiles. The role of auxin in the process appeared negligible. Moreover, the results suggested that oxidative stress imposed by JG and LW was one of mechanisms of allelopathic action of the studied quinones in plants. © The Authors 2016.},

note = {16},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84943329529,

title = {Effect of trimethyltin chloride on slow vacuolar (SV) channels in vacuoles from red beet (Beta vulgaris L.) taproots},

author = { Z. Trela and Z. Burdach and A. Siemieniuk and S. Przestalski and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943329529&doi=10.1371%2fjournal.pone.0136346&partnerID=40&md5=de049663a59a03f2b71dada43afadf3c},

doi = {10.1371/journal.pone.0136346},

issn = {19326203},

year  = {2015},

date = {2015-01-01},

journal = {PLoS ONE},

volume = {10},

number = {8},

publisher = {Public Library of Science},

abstract = {In the present study, patch-clamp techniques have been used to investigate the effect of trimethyltin chloride (Met3SnCl) on the slow vacuolar (SV) channels in vacuoles from red beet (Beta vulgaris L.) taproots. Activity of SV channels has been measured in whole-vacuole and cytosolic side-out patch configurations. It was found that addition of trimethyltin chloride to the bath solution suppressed, in a concentration-dependent manner, SV currents in red beet vacuoles. The time constant, τ, increased significantly in the presence of the organotin. When single channel activity was analyzed, only little channel activity could be recorded at 100 μM Met3SnCl. Trimethyltin chloride added to the bath medium significantly decreased (by ca. threefold at 100 μM Met3SnCl and at 100 mV voltage; as compared to the control medium) the open probability of single channels. Single channel recordings obtained in the presence and absence of trimethyltin chloride showed that the organotin only slightly (by <10%) decreased the unitary conductance of single channels. It was also found that Met3SnCl significantly diminished the number of SV channel openings, whereas it did not change the opening times of the channels. Taking into account the above and the fact that under the here applied experimental conditions (pH = 7.5) Met3SnCl is a non-dissociated (more lipophilic) compound, we suggest that the suppression of SV currents observed in the presence of the organotin results probably from its hydrophobic properties allowing this compound to translocate near the selectivity filter of the channel. © 2015 Trela et al.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84940063294,

title = {Expansion time of hot nuclei produced by a relativistic deuteron beam},

author = { V.A. Karnaukhov and S.P. Avdeyev and W. Karcz and V.V. Kirakosyan and P.A. Rukoyatkin and V.I. Stegaylov and H. Oeschler and A.S. Botvina},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940063294&doi=10.1134%2fS1063778815040079&partnerID=40&md5=2eddfb95fe2c880b752aeab807b5a4ac},

doi = {10.1134/S1063778815040079},

issn = {10637788},

year  = {2015},

date = {2015-01-01},

journal = {Physics of Atomic Nuclei},

volume = {78},

number = {5},

pages = {574-578},

publisher = {Maik Nauka-Interperiodica Publishing},

abstract = {The multifragmentation time scale is measured for d(4.4 GeV) + Au collisions by the analysis of the relative angle correlation function for the intermediate-mass fragments. The experiment was performed with the FASA 4π setup installed at the external beam of the superconducting accelerator Nuclotron. A combined approach of intranuclear cascade prescription followed by the Statistical Model of Multifragmentation is used for the analysis of the data. Multifragmentation of a target spectator is measured to be 100 fm/c (CL > 99.5%) delayed in relation to the collision moment. The latter is fixed by the registration of the fast fragment with Z = 4, produced at the collisionmoment. © 2015, Pleiades Publishing, Ltd.},

note = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84939809954,

title = {Effect of K+ and Ca2+ on the indole-3-acetic acidand fusicoccin-induced growth and membrane potential in maize coleoptile cells},

author = { A. Siemieniuk and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939809954&doi=10.1093%2faobpla%2fplv070&partnerID=40&md5=0836136a16471c58c62077558f2284ed},

doi = {10.1093/aobpla/plv070},

issn = {20412851},

year  = {2015},

date = {2015-01-01},

journal = {AoB PLANTS},

volume = {7},

number = {1},

publisher = {Oxford University Press},

abstract = {The role of potassium (K+) and calcium (Ca2+) in the regulation of plant growth and development is complex and needs a diverse range of physiological studies. Both elements are essential for satisfactory crop production. Here, the effects of K+ and Ca2+ ions on endogenous growth and growth in the presence of either indole-3-acetic acid (IAA) or fusicoccin (FC) were studied in maize (Zea mays) coleoptiles. Membrane potentials of coleoptile parenchymal cells, incubated in media containing IAA, FC and different concentrations of K+ and Ca2+, were also determined. Growth experiments have shown that in the absence of K+ in the incubation medium, both endogenous and IAAor FC-induced growth were significantly inhibited by 0.1 and 1 mM Ca2+, respectively, while in the presence of 1 mM K+ they were inhibited only by 1 mM Ca2+. At 10mM K+, endogenous growth and growth induced by either IAA or FC did not depend on Ca2+ concentration. TEA-Cl, a potassium channel blocker, added 1 h before IAA or FC, caused a reduction of growth by 59 or 45 %, respectively. In contrast to TEA-Cl, verapamil, the Ca2+ channel blocker, did not affect IAA- and FC-induced growth. It was also found that in parenchymal cells of maize coleoptile segments, membrane potential (Em) was strongly affected by the mediumK+, independently of Ca2+. However, lack of Ca2+ in the incubation medium significantly reduced the IAA- and FC-induced membrane potential hyperpolarization. TEA-Cl applied to the control medium in the same way as in growth experiments caused Em hyperpolarization synergistic with hyperpolarization produced by IAA or FC. Verapamil did not change either the Em of parenchymal cells incubated in the control medium or the IAA- and FC-induced membrane hyperpolarization. The data presented here have been discussed considering the role of K+ uptake channels in regulation of plant cell growth. © The Authors 2015.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84922881434,

title = {Role of chloride ions in the promotion of auxin-induced growth of maize coleoptile segments},

author = { Z. Burdach and R. Kurtyka and A. Siemieniuk and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84922881434&doi=10.1093%2faob%2fmcu170&partnerID=40&md5=7ef4c10f60d347b453e47c8f373ba816},

doi = {10.1093/aob/mcu170},

issn = {03057364},

year  = {2014},

date = {2014-01-01},

journal = {Annals of Botany},

volume = {114},

number = {5},

pages = {1023-1034},

publisher = {Oxford University Press},

abstract = {Background and Aims: The mechanism of auxin action on ion transport in growing cells has not been determined in detail. In particular, little is known about the role of chloride in the auxin-induced growth of coleoptile cells. Moreover, the data that do exist in the literature are controversial. This study describes experiments that were carried out with maize (Zea mays) coleoptile segments, this being a classical model system for studies of plant cell elongation growth. • Methods: Growth kinetics or growth and pH changes were recorded in maize coleoptiles using two independent measuring systems. The growth rate of the segments was measured simultaneously with medium pH changes. Membrane potential changes in parenchymal cells of the segments were also determined for chosen variants. The question of whether anion transport is involved in auxin-induced growth of maize coleoptile segmentswas primarily studied using anion channel blockers [anthracene-9-carboxylic acid (A-9-C) and 4;4' -diisothiocyanatostilbene-2;2'-disulphonic acid (DIDS)]. In addition, experiments in which KCl was replaced by KNO3 were also performed. • Key Results: Both anion channel blockers, added at 0.1 mM, diminished indole-3-acetic acid (IAA)-induced elongation growth by ∼ 30%. Medium pH changes measured simultaneously with growth indicated that while DIDS stopped IAA-induced proton extrusion, A-9-C diminished it by only 50%. Addition of A-9-C to medium containing 1 mM KCl did not affect the characteristic kinetics of IAA-induced membrane potential changes, while in the presence of 10 mM KCl the channel blocker stopped IAA-induced membrane hyperpolarization. Replacement of KCl with KNO3 significantly decreased IAA-induced growth and inhibited proton extrusion. In contrast to the KCl concentration, the concentration of KNO3 did not affect the growth-stimulatory effect of IAA. For comparison, the effects of the cation channel blocker tetraethylammonium chloride (TEA-Cl) on IAA-induced growth and proton extrusion were also determined. TEA-Cl, added 1 h before IAA, caused reduction of growth by 49.9% and inhibition of proton extrusion. • Conclusions: These results suggest that Cl - plays a role in the IAA-induced growth of maize coleoptile segments. A possible mechanism for Cl- uptake during IAA-induced growth is proposed in which uptake of K+ and Cl- ions in concert with IAA-induced plasma membrane H+ -ATPase activity changes the membrane potential to a value needed for turgor adjustment during the growth of maize coleoptile cells. © The Author 2014.},

note = {18},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84897114508,

title = {Electrical properties of deuteron irradiated high resistivity silicon},

author = { J. Krupka and W. Karcz and S.P. Avdeyev and P. Kamiński and R. Kozłowski},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897114508&doi=10.1016%2fj.nimb.2014.01.021&partnerID=40&md5=351418c864f1fdfcb1e864bdfdcb2106},

doi = {10.1016/j.nimb.2014.01.021},

issn = {0168583X},

year  = {2014},

date = {2014-01-01},

journal = {Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms},

volume = {325},

pages = {107-114},

publisher = {Elsevier B.V.},

abstract = {We have investigated resistivity changes introduced on the high-resistivity p-type silicon wafer by the irradiation with deuteron beam with an energy of 4.4 GeV performed in the NUCLOTRON superconducting accelerator. Two contactless techniques were used for the measurements of resistivity changes: namely the microwave split post dielectric resonator (SPDR) technique and capacitance measurements in the frequency domain. The first technique allows resistivity measurements in the plane of the wafer, while the second one in the direction perpendicular to the wafer. The resistivity map obtained with the SPDR technique enabled us to obtain a permanent fingerprint of the accelerator beam intensity profile. It has been shown that after the irradiation, the material resistivity increased to ∼3.9 × 105 Ω cm in the wafer region exposed to the maximum beam intensity. Complementary studies of the properties and concentrations of radiation deep-level defects were performed by the high-resolution photo-induced current transient spectroscopy (HRPITS). These studies have shown that the irradiation of the high resistivity silicon with 4.4-GeV deuterons results in the formation of several types of deep-level defects responsible for the charge compensation. © 2014 Elsevier B.V. All rights reserved.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84895745980,

title = {Cellular responses to naphthoquinones: Juglone as a case study},

author = { M. Rudnicka and M. Polak and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84895745980&doi=10.1007%2fs10725-013-9855-y&partnerID=40&md5=314ebb826b8ec7b8e6e84e571cd491fe},

doi = {10.1007/s10725-013-9855-y},

issn = {01676903},

year  = {2014},

date = {2014-01-01},

journal = {Plant Growth Regulation},

volume = {72},

number = {3},

pages = {239-248},

publisher = {Kluwer Academic Publishers},

abstract = {The effects of juglone (JG) on the endogenous growth, growth in the presence of either indoleacetic acid (IAA) or fusicoccin (FC) and on proton extrusion were studied in maize coleoptile segments. In addition, membrane potential changes were also determined at chosen JG concentrations. It was found that JG, when added to the incubation medium, inhibited endogenous growth as well as growth in the presence of either IAA or FC. Simultaneous measurements of growth and external pH indicated that inhibition of either IAA-induced growth or proton extrusion by JG was a linear function of JG concentration. Addition of JG to the control medium caused depolarization of the membrane potential (Em), value of which was dependent on JG concentration and time after its administration. Hyperpolarization of Em induced by IAA was suppressed in the presence of JG. It was also found that for coleoptile segments initially preincubated with JG, although subsequently removed, addition of IAA was not effective in the stimulation of growth and medium acidification. Taken together, these results suggest that the mechanism by which JG inhibits the IAA-induced growth of maize coleoptile segments involves inhibition of PM H+-ATPase activity. © 2013 The Author(s).},

note = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84855236421,

title = {Effect of thiosulphinates contained in garlic extract on growth, proton fluxes and membrane potential in maize (Zea mays L.) coleoptile segments},

author = { M. Polak and W. Zaborska and Z. Tukaj and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855236421&doi=10.1007%2fs11738-011-0803-z&partnerID=40&md5=d206b6dc2f70b59b753d6b789e333eb0},

doi = {10.1007/s11738-011-0803-z},

issn = {01375881},

year  = {2012},

date = {2012-01-01},

journal = {Acta Physiologiae Plantarum},

volume = {34},

number = {1},

pages = {41-52},

abstract = {The effect of thiosulphinates contained in garlic extract (GE) on endogenous growth, growth in the presence of either indoleacetic acid (IAA) or fusicoccin (FC), and proton extrusion in maize coleoptile segments were studied. In addition, membrane potential changes at some GE dilutions and the protective effect of dithiothreitol (DTT) against GE toxicity were also determined. It was found that GE at almost all dilutions studied, when added to the incubation medium inhibited endogenous growth as well as growth in the presence of either IAA or FC. Simultaneous measurements of growth and external pH indicated that the administration of GE resulted in a complex change in the pH of the external medium; after an initial transient acidification, pH increased and reached the maximal value followed by a gradual decrease of medium pH. When IAA or FC was added after preincubation of the segments in the presence of GE the changes in medium pH were not significantly different from these obtained with GE only. If the coleoptile segments were first preincubated with GE and subsequently GE was removed, the addition of IAA induced strong growth and medium acidification. Dithiothreitol added together with GE neutralized the toxic effect of GE on growth of coleoptile segments incubated in the presence of IAA. The addition of GE to the control medium caused a depolarization of the membrane potential, the value of witch depended on GE dilution. These results indicate that the toxic effect of GE on growth of plant cells might be caused by disruption of the catalytic function of the plasma membrane H +-ATPase on formation of the disulfide bonds. © 2011 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84872154905,

title = {Effect of trimethyllead chloride on slowly activating (SV) channels in red beet (Beta vulgaris L.) taproots},

author = { Z. Trela and Z. Burdach and S. Przestalski and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872154905&doi=10.1016%2fj.crvi.2012.11.004&partnerID=40&md5=123a4c36295b86d469fe5585a393af80},

doi = {10.1016/j.crvi.2012.11.004},

issn = {16310691},

year  = {2012},

date = {2012-01-01},

journal = {Comptes Rendus - Biologies},

volume = {335},

number = {12},

pages = {722-730},

abstract = {The patch-clamp technique was used to examine the effect of trimethyllead chloride (Met3PbCl) on SV channel activity in red beet (Beta vulgaris L.) taproot vacuoles. It was found that in the control bath the macroscopic currents showed the typical slow activation and a strong outward rectification of the steady-state currents. An addition of Met3PbCl to the bath solution blocked, in a concentration-dependent manner, SV currents in red beet vacuoles. The time constant τ increased several times in the presence of 100 μM trimethyllead chloride at all voltages tested. When single channel properties were analyzed, only little channel activity could be recorded in the presence of 100 μM Met3PbCl. Trimethyllead chloride decreased significantly (by about one order of magnitude) the open probability of single channels. The recordings of single channel activity obtained in the presence and absence of Met3PbCl showed that organolead only slightly (by ca. 10%) decreased the unitary conductance of single channels. It was also found that Met3PbCl diminished significantly the number of SV channel openings, whereas it did not change the opening times of the channels. Taken together, these results suggest that Met3PbCl binding site is located outside the channel selectivity filter. © 2012 Académie des sciences.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84869187279,

title = {Time scale of nuclear multifragmentation induced by light relativistic ions},

author = { V.A. Karnaukhov and S.P. Avdeyev and A.S. Botvina and V.V. Kirakosyan and O.V. Strekalovsky and P.A. Rukoyatkin and W. Karcz and E. Norbeck and H. Oeschler},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869187279&doi=10.1088%2f0954-3899%2f39%2f12%2f125104&partnerID=40&md5=b2a09b84fc93148bca80bf96b86eda69},

doi = {10.1088/0954-3899/39/12/125104},

issn = {09543899},

year  = {2012},

date = {2012-01-01},

journal = {Journal of Physics G: Nuclear and Particle Physics},

volume = {39},

number = {12},

abstract = {Nuclear fragmentation in a d (4.4 GeV)+Au collision was studied with a 4π setup FASA on the external beam of the Dubna Nuclotron. The IMF-IMF relative angle correlation function was analyzed using the statistical model of multifragmentation (SMM) with the main goal of estimating the total time scale of the process. It was found that the fragmentation of the hot nucleus was ∼100 fm/c delayed in respect to the collision moment. © 2012 IOP Publishing Ltd.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-84861099047,

title = {Interactive effects of temperature and heavy metals (Cd, Pb) on the elongation growth in maize coleoptiles},

author = { R. Kurtyka and E. Małkowski and Z. Burdach and A. Kita and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861099047&doi=10.1016%2fj.crvi.2012.03.012&partnerID=40&md5=4346ff3f622d042a44a2211c75624cb4},

doi = {10.1016/j.crvi.2012.03.012},

issn = {16310691},

year  = {2012},

date = {2012-01-01},

journal = {Comptes Rendus - Biologies},

volume = {335},

number = {4},

pages = {292-299},

abstract = {The effect of Cd and Pb on endogenous and IAA-induced elongation growth and medium pH of maize coleoptile segments incubated at 20, 25 and 30 °C was studied. It was found that the elongation of coleoptile segments and proton extrusion increased with the temperature and reached its maximum at 30 °C. For Cd, the maximal inhibition of endogenous and IAA-induced growth as well as medium acidification of coleoptile segments was observed at 25 °C. Meanwhile, Pb, irrespective of the temperature, diminished the growth of the segments by ca. 20%, increasing the acidification of the incubation medium. It was also found that in contrast to Cd, Pb accumulation in maize coleoptile segments did not correlate with temperature. The results suggest that the toxic effect of Cd on elongation growth of coleoptile segments is connected with the decrease of the PM H +-ATPase activity and probably with Cd-induced high acivity of IAA oxidase, whereas the effect of Pb did not depend on activity of any of the enzymes. © 2012 Académie des sciences.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-81755171203,

title = {Fusicoccin counteracts the toxic effect of cadmium on the growth of maize coleoptile segments},

author = { R. Kurtyka and A. Kita and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-81755171203&doi=10.1007%2fs00244-011-9662-2&partnerID=40&md5=4a1349e5ff8e2335f2c1ddcb81ccd1d2},

doi = {10.1007/s00244-011-9662-2},

issn = {00904341},

year  = {2011},

date = {2011-01-01},

journal = {Archives of Environmental Contamination and Toxicology},

volume = {61},

number = {4},

pages = {568-577},

abstract = {The effects of cadmium (Cd; 0.1-1000 μM) and fusicoccin (FC) on growth, Cd 2+ content, and membrane potential (E m) in maize coleoptile segments were studied. In addition, the E m changes and accumulation of Cd and calcium (Ca) in coleoptile segments treated with Cd 2+ combined with 1 μM FC or 30 mM tetraethylammonium (TEA) chloride (K +-channel blocker) were also determined. In this study, the effects of Ca 2+-channel blockers [lanthanum (La) and verapamil (Ver)] on growth and content of Cd 2+ and Ca 2+ in coleoptile segments were also investigated. It was found that Cd at high concentrations (100 and 1000 μM) significantly inhibited endogenous growth of coleoptile segments and simultaneously measured proton extrusion. FC combined with Cd 2+ counteracted the toxic effect of Cd 2+ on endogenous growth and significantly decreased Cd 2+ content (not the case for Cd 2+ at the highest concentration) in coleoptile segments. Addition of Cd to the control medium caused depolarization of E m, the extent of which was dependent on Cd concentration and time of treatment with Cd 2+. Hyperpolarization of E m induced by FC was suppressed in the presence of Cd 2+ at 1000 μM but not Cd 2+ at 100 μM. It was also found that treatment of maize coleoptile segments with 30 mM TEA chloride caused hyperpolarization of E m and decreased Cd 2+ content in coleoptile segments, suggesting that, in the same way as for FC, accumulation of Cd 2+ was dependent on plasma membrane (PM) hyperpolarization. Similar to FC, TEA chloride also decreased Ca 2+ content in coleoptile segments. La and Ver combined with Cd 2+ (100 μM) significantly decreased Cd content in maize coleoptile segments, but only La completely abolished the toxic effect of Cd 2+ on endogenous growth and growth in the presence of FC. Taken together, these results suggest that the mechanism by which FC counteracts the toxic effect of Cd 2+ (except at 1000 μM Cd 2+) on the growth of maize coleoptile segments involves both stimulation of PM H +-ATPase activity by FC as well as Cd 2+-permeable, voltage-dependent Ca channels, which are blocked by FC and TEA chloride-induced PM hyperpolarization. © 2011 The Author(s).},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-80054026320,

title = {Temperature dependence of growth in maize seedlings and excised coleoptile segments},

author = { R. Kurtyka and Z. Burdach and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80054026320&partnerID=40&md5=81d96c75404438c30eec0a973b6ba843},

issn = {12301485},

year  = {2011},

date = {2011-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {20},

number = {5},

pages = {1221-1225},

abstract = {The effect of temperature in the range 22-32°C and 25-40°C on elongation growth of maize seedlings and coleoptiles excised from them was studied, respectively. It was found that elongation of maize seedling's organs (root; mesocotyl and coleoptile) increased with increasing temperature in the range 22-32°C. However, when coleoptile segments excised from seedlings grown at highest temperature (32°C) were incubated at various temperatures (25; 30; 35; and 40°C) and in the presence of IAA their elongation growth was always lower compared to segments excised from seedlings grown at 22 and 27°C. Simultaneous measurements of elongation growth and external medium pH indicated that proton extrusion increased with increasing medium temperature from 25 to 35°C. The results presented here show that the temperatures at which the maize seedlings were cultivated are crucial for temperature dependence of a coleoptile segment's elongation growth.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-80052821764,

title = {Electromagnetic properties of sapphire, ruby, and irradiated ruby at frequencies of 30-40 GHz},

author = { J. Krupka and T. Shimada and I. Ueda and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052821764&doi=10.1063%2f1.3635787&partnerID=40&md5=61370c9600bc7949b79433598a57f5b7},

doi = {10.1063/1.3635787},

issn = {00036951},

year  = {2011},

date = {2011-01-01},

journal = {Applied Physics Letters},

volume = {99},

number = {10},

abstract = {Permittivity and electromagnetic losses of sapphire, ruby, and irradiated ruby samples were examined at frequencies of 30-40 GHz and temperatures of 4-300 K employing the whispering gallery mode technique. At room temperature, the dielectric properties of the sapphire, ruby, and irradiated ruby samples were similar. Significant differences in electromagnetic losses took place at cryogenic temperatures. Losses were related to the imaginary part of the susceptibility associated with the presence of paramagnetic Cr3+ ions as well as those introduced by structural defects caused by the irradiation of ruby samples. The presence of impurities and the structural defects result in the dielectric losses being larger than those of pure sapphire. © 2011 American Institute of Physics.},

note = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79953688700,

title = {Effect of cadmium and lead on the membrane potential and photoelectric reaction of Nitellopsis obtusa cells},

author = { R. Kurtyka and Z. Burdach and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79953688700&doi=10.4149%2fgpb_2011_01_52&partnerID=40&md5=a317763ee317c849a0eafef55067489b},

doi = {10.4149/gpb_2011_01_52},

issn = {02315882},

year  = {2011},

date = {2011-01-01},

journal = {General Physiology and Biophysics},

volume = {30},

number = {1},

pages = {52-58},

abstract = {The effects of Cd and Pb on membrane potential (Em) and photoelectric reaction of Nitellopsis obtusa cells were investigated. It was found that Cd and Pb at 1.0 mM caused a depolarization of the Em, whereas both metals at lower concentrations changed the Em in a different way. Pb at 0.1 mM and 0.01 mM hyperpolarized the Em, whereas Cd at the same concentrations depolarized and did not change the E m, respectively. In the presence of 0.01 mM Pb, the light-induced hyperpolarization of the Em was by 18% higher as compared to the control, whereas at 1.0 mM Pb it was by 40% lower. Pb at 0.1 mM and Cd at 0.01 mM or 5 x 0.01 mM did not change the light-induced membrane hyperpolarization. However, in the presence of Cd at 0.1 mM and 1.0 mM this hyperpolarization was 2-fold lower or was completely abolished, respectively. These results suggest that at high Cd and Pb concentrations both depolarization of the Em and decrease of light-induced membrane hyperpolarization in Nitellopsis obtusa cells are probably due to inhibition of the plasma membrane H+-ATPase activity, whereas both metals at lower concentrations differ in mechanism of membrane potential changes.},

note = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79951771728,

title = {Effect of temperature on the dose-response curves for auxin-induced elongation growth in maize coleoptile segments},

author = { M. Polak and Z. Tukaj and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951771728&doi=10.1007%2fs11738-010-0563-1&partnerID=40&md5=cf663620e1344c7efed3f330850bd4d5},

doi = {10.1007/s11738-010-0563-1},

issn = {01375881},

year  = {2011},

date = {2011-01-01},

journal = {Acta Physiologiae Plantarum},

volume = {33},

number = {2},

pages = {437-442},

abstract = {The dose-response curves for IAA-induced growth in maize coleoptile segments were studied as a function of time and temperature. In addition, the kinetics of growth rate responses at some auxin concentrations and temperatures was also compared. It was found that the dose-response curves for IAA-induced elongation growth were, independently of time and temperature, bell-shaped with an optimal concentration at 10-5 M IAA. The kinetics of IAA-induced growth rate responses depended on IAA concentration and temperature, and could be separated into two phases (biphasic reaction). The first phase (very rapid) was followed by a long lasting one (second phase), which began about 30 min after auxin addition. For coleoptile segments incubated at 30°C, the amplitudes of the first and second phase were significantly higher, when compared with 25°C, at all IAA concentrations studied. However, when coleoptile segments were incubated at 20°C, the elongation growth of coleoptile segments treated with suboptimal IAA concentrations was diminished, mainly as a result of both phases reduction. In conclusion, we propose that the shape of the dose-response curves for IAA-induced growth in maize coleoptile segments is connected with biphasic kinetic of growth rate response. © 2010 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.},

note = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-79551701926,

title = {Collective flow in nuclear fragmentation induced by 4.4 GeV deuteron on gold target},

author = { V.A. Karnaukhov and S.P. Avdeyev and H. Oeschler and V.V. Kirakosyan and P.A. Rukoyatkin and A. Budzanowski and W. Karcz and E. Norbeck and A.S. Botvina},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79551701926&doi=10.1134%2fS1547477111010067&partnerID=40&md5=0ab35164e89c084dd802437bb724419e},

doi = {10.1134/S1547477111010067},

issn = {15474771},

year  = {2011},

date = {2011-01-01},

journal = {Physics of Particles and Nuclei Letters},

volume = {8},

number = {1},

pages = {19-22},

abstract = {Nuclear multifragmentation in d (4.4 GeV) + Au collision was studied with the 4π setup FASA installed at the external beam of the Dubna Nuclotron. Data obtained are analyzed within the statistical model of multifragmentation. It is found that the kinetic energy spectra of intermediate mass fragments deviate from the predicted ones. It is explained by the collective flow caused by the thermal expansion of fragmenting nucleus. © 2011 Pleiades Publishing, Ltd.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77953850580,

title = {Multifragmentation in collitions of 4.4 GeV-deuterons with gold target},

author = { S.P. Avdeyev and V.A. Karnaukhov and H. Oeschler and V.V. Kirakosyan and P.A. Rukoyatkin and A. Budzanowski and W. Karcz and E. Norbeck and A.S. Botvina},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953850580&doi=10.1142%2fS0218301310015618&partnerID=40&md5=5f91fa352d236a41a703aafdeb3e19dd},

doi = {10.1142/S0218301310015618},

issn = {02183013},

year  = {2010},

date = {2010-01-01},

journal = {International Journal of Modern Physics E},

volume = {19},

number = {5-6},

pages = {1155-1161},

abstract = {The relative velocity correlation function of pairs of intermediate mass fragments has been studied for d+Au collisions at 4.4 GeV. Experimental correlation functions are compared to that obtained by multibody Coulomb trajectory calculations under the assumption of various decay times of the fragmenting system. The combined approach with the empirically modified intranuclear cascade code followed by the statistical multifragmentation model was used to generate the starting conditions for these calculations. The fragment emission time is found to be less than 40 fm/c. © 2010 World Scientific Publishing Company.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77949916345,

title = {5-Hydroxy-1,4-naphthoquinone (juglone) and 2-hydroxy-1,4-naphthoquinone (lawsone) influence on jack bean urease activity: Elucidation of the difference in inhibition activity},

author = { M. Kot and W. Karcz and W. Zaborska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77949916345&doi=10.1016%2fj.bioorg.2010.02.002&partnerID=40&md5=6cd4e38a38b09b529ba4145fd7b04157},

doi = {10.1016/j.bioorg.2010.02.002},

issn = {00452068},

year  = {2010},

date = {2010-01-01},

journal = {Bioorganic Chemistry},

volume = {38},

number = {3},

pages = {132-137},

abstract = {The aim of this study was elucidation of the difference in inhibition influence of 5-hydroxy-1,4-naphthoquinone (juglone) and 2-hydroxy-1,4-naphthoquinone (lawsone) on jack bean urease activity. It was found that juglone acted as a strong, time and concentration dependent inactivator of urease. On the contrary, lawsone showed an inconsiderable inhibition influence. The reactivation of juglone modified urease showed the participation of reversible and irreversible contribution in the inactivation. In the presence of an excess of DTT, urease inactivated by juglone regained 70% of its activity. The reversible inactivation was attributed to oxidation of the essential urease thiols by reactive oxygen species (ROS) realizing during reduction of juglone to seminaphthoquinone. Presence of hydrogen peroxide in the incubation system was proved by direct determination and by application of catalase. The irreversible contribution in the inhibition was assumed as an arylation of urease thiol groups by juglone. The insignificant urease inhibition by lawsone was concluded as an effect of a low hydrogen peroxide generation and lawsone resistance for reaction with protein thiols. It was found that lawsone well reacted with l-cysteine, poorly with glutathione and hardly with urease thiols. The observed sequence was arranged according the rule the more complex thiol the less susceptible for reaction with lawsone. On the other hand, juglone displayed an excellent reactivity towards both thiols and urease. Thus, this indicated a significance of a steric hindrance which appeared when the hydroxyl group changing position from 5 in juglone (5-hydroxy-1;4-naphthoquinone) to 2 in lawsone (2-hydroxy-1;4-naphthoquinone). © 2010 Elsevier Inc. All rights reserved.},

note = {46},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-77953349299,

title = {Emission time of the intermediate mass fragments in collisions of relativistic deuterons with the gold target},

author = { S.P. Avdeyev and V.A. Karnaukhov and H. Oeschler and V.V. Kirakosyan and P.A. Rukoyatkin and A. Budzanowski and W. Karcz and E. Norbeck and A.S. Botvina},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953349299&doi=10.3103%2fS1062873810040295&partnerID=40&md5=fc9306f9ac860716b00f5b0e2a7d2d0e},

doi = {10.3103/S1062873810040295},

issn = {10628738},

year  = {2010},

date = {2010-01-01},

journal = {Bulletin of the Russian Academy of Sciences: Physics},

volume = {74},

number = {4},

pages = {559-563},

abstract = {The relative velocity correlation function of pairs of intermediate mass fragments has been studied for d + Au collisions at 4.4 GeV. Experimental correlation functions are compared to that obtained by multi-body Coulomb trajectory calculations under the assumption of various decay times of the fragmenting system. The combined approach with the empirically modified intranuclear cascade code followed by the statistical multifragmentation model was used to generate the starting conditions for these calculations. The fragment emission time is found to be less than 40 fm c-1. © Allerton Press, Inc., 2010.},

note = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-85027942706,

title = {Superstatistics analysis of the ion current distribution function: Met3PbCl influence study.},

author = { J. Miśkiewicz and Z. Trela and S. Przestalski and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027942706&doi=10.1007%2fs00249-010-0594-y&partnerID=40&md5=fddf5c354d389882370c2257a76279be},

doi = {10.1007/s00249-010-0594-y},

issn = {14321017},

year  = {2010},

date = {2010-01-01},

journal = {European biophysics journal : EBJ},

volume = {39},

number = {10},

pages = {1397-1406},

abstract = {A novel analysis of ion current time series is proposed. It is shown that higher (second; third and fourth) statistical moments of the ion current probability distribution function (PDF) can yield new information about ion channel properties. The method is illustrated on a two-state model where the PDF of the compound states are given by normal distributions. The proposed method was applied to the analysis of the SV cation channels of vacuolar membrane of Beta vulgaris and the influence of trimethyllead chloride (Met(3)PbCl) on the ion current probability distribution. Ion currents were measured by patch-clamp technique. It was shown that Met(3)PbCl influences the variance of the open-state ion current but does not alter the PDF of the closed-state ion current. Incorporation of higher statistical moments into the standard investigation of ion channel properties is proposed.},

note = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-47749141457,

title = {Modification of jack bean urease thiols by thiosulphinates contained in garlic extract: DTNB titration studies},

author = { W. Zaborska and W. Karcz and M. Kot and A. Juszkiewicz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-47749141457&doi=10.1016%2fj.foodchem.2008.05.029&partnerID=40&md5=dac671bec0e7f0f37fadc4eac06c01e0},

doi = {10.1016/j.foodchem.2008.05.029},

issn = {03088146},

year  = {2009},

date = {2009-01-01},

journal = {Food Chemistry},

volume = {112},

number = {1},

pages = {42-45},

abstract = {The reactivity of the thiol groups in urease with thiosulphinate contained in garlic extract was spectroscopically characterised. The enzyme was incubated with the garlic extract and the reaction progress curves were recorded in the presence of thiol-selective reagent 5,5′-dithiobis(2-nitrobenzoic acid). Simultaneously the enzyme residual activity was also determined. The process was studied in 50 mM phosphate buffer, at pH 7.8 and ambient temperature. It was found that thiosulphinates act as time- and concentration-dependent inactivators of urease. The observed decrease of the enzyme activity corresponds to the number of the urease thiols modified by thiosulphinates. The modification of half of all the urease thiols (18 of 36) causes a slight (only 8-10%) decrease of the catalytic activity. The modification of the remaining 18 thiols results in significant disturbance of urease action until complete loss of the catalytic function occurs. This provides the evidence that Cys592, the critical residue for urease activity, belongs to the enzyme thiols, which are less reactive and more resistant to chemical modification than the other thiols. © 2008 Elsevier Ltd. All rights reserved.},

note = {7},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-58449131037,

title = {Critical temperature for the nuclear liquid-gas phase transition (from multifragmentation and fission)},

author = { V.A. Karnaukhov and H. Oeschler and A. Budzanowski and S.P. Avdeyev and A.S. Botvina and E.A. Cherepanov and W. Karcz and V.V. Kirakosyan and P.A. Rukoyatkin and I. Skwirczyńska and E. Norbeck},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-58449131037&doi=10.1134%2fS1063778808120077&partnerID=40&md5=97358935d422f443f1f40abd0bf1a997},

doi = {10.1134/S1063778808120077},

issn = {10637788},

year  = {2008},

date = {2008-01-01},

journal = {Physics of Atomic Nuclei},

volume = {71},

number = {12},

pages = {2067-2073},

abstract = {Critical temperature T c for the nuclear liquid-gas phase transition is estimated from both the multifragmentation and fission data. In the first case, the critical temperature is obtained by analysis of the intermediate-mass-fragment yields in p(8.1 GeV) + Au collisions within the statistical model of multifragmentation. In the second case, the experimental fission probability for excited 188Os is compared with the calculated one with T c as a free parameter. It is concluded for both cases that the critical temperature is higher than 15 MeV. © 2008 Pleiades Publishing, Ltd.},

note = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-42349112580,

title = {The upgraded FASA setup for studying nuclear multifragmentation},

author = { V.V. Kirakosyan and A.V. Simonenko and S.P. Avdeev and V.A. Karnaukhov and W. Karcz and I. Skwirczyńska and B. Czech and H. Oeschler},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-42349112580&doi=10.1007%2fs10786-008-2001-9&partnerID=40&md5=be9f036da74e60b6578781017d1597e3},

doi = {10.1007/s10786-008-2001-9},

issn = {00204412},

year  = {2008},

date = {2008-01-01},

journal = {Instruments and Experimental Techniques},

volume = {51},

number = {2},

pages = {159-165},

abstract = {The upgraded FASA 4π setup for studying target multifragmentation induced by relativistic light ions on the Nuclotron superconducting accelerator at the Joint Institute for Nuclear Research is described. Basic attention is concentrated on a new detector module composed of 25 telescope-spectrometers. This detector array is used to measure correlations between the relative velocities of fragments with intermediate masses, which is necessary for investigation of multifragmentation dynamics. © 2008 MAIK Nauka.},

note = {9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-38949197090,

title = {Effect of calcium and cadmium on growth and accumulation of cadmium, calcium, potassium and sodium in maize seedlings},

author = { R. Kurtyka and E. Małkowski and A. Kita and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-38949197090&partnerID=40&md5=82815e4249ff6d2a432ee031e3d217bc},

issn = {12301485},

year  = {2008},

date = {2008-01-01},

journal = {Polish Journal of Environmental Studies},

volume = {17},

number = {1},

pages = {51-56},

abstract = {We examined cadmium (0.1 mmol/1) toxicity and effect of different calcium concentrations (0.1;1.0; and 10.0 mmol/1) on the growth of organs (root; mesocotyl and coleoptile) of 4-day-old maize seedlings in hydroponic cultures. The influence of both metals on distribution of cadmium, calcium, potassium and sodium in the organs of maize seedlings was also studied. Calcium at a concentration of 1.0 mmol/l increased the growth of all organs in comparison with the growth in 0.1 and 10.0 mmol/ l CaCl2. Cadmium inhibited maize organ growth in the presence of 0.1 and 1.0 mmol/l CaCl2. whereas cadmium toxicity was not observed at 10.0 mmol/l CaCl2. It was found that the content of both metals in maize seedling organs rose with increasing concentrations of Ca or Cd in the hydroponic solutions. Cadmium administration together with 10.0 mmol/l CaCl2 led to an increase in calcium concentrations in roots and coleoptiles. The highest calcium concentration (10.0 mmol/1) caused a decrease in the cadmium concentration in roots. The addition of cadmium with 0.1 or 1.0 mmol/l CaCl2 diminished potassium accumulation in the roots. By contrast, potassium content in the roots was not altered by cadmium when calcium was applied at a concentration of 10.0 mmol/l CaCl2. Sodium content in maize seedlings was not affected in the presence of both cadmium and calcium at a concentration of 0.1 or 1.0 mmol/l. whereas cadmium with 10.0 mmol/l CaCl2 decreased sodium accumulation in the roots. Thus, we conclude that the positive effect of highest concentration of calcium on the growth in the presence of Cd relay on the maintenance of high concentration of potassium in the roots.},

note = {43},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-36448954684,

title = {Effect of cadmium on growth, proton extrusion and membrane potential in maize coleoptile segments},

author = { W. Karcz and R. Kurtyka},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-36448954684&doi=10.1007%2fs10535-007-0147-0&partnerID=40&md5=ee785612d5a5ef5bb8bb65233cf2a9ab},

doi = {10.1007/s10535-007-0147-0},

issn = {00063134},

year  = {2007},

date = {2007-01-01},

journal = {Biologia Plantarum},

volume = {51},

number = {4},

pages = {713-719},

abstract = {Cd accumulation, its effects on elongation growth of maize coleoptile segments, pH changes of their incubation medium and the membrane potential of parenchymal cells were studied. The Cd content increased significantly with exposure to increasing cadmium concentrations. Coleoptile segments accumulated the metal more efficiently in the range 10-100 μM Cd, than in the range 100-1000 μM Cd. Cd at concentrations higher than 1.0 μM produced a significant inhibition of both growth and proton extrusion. 100 μM Cd caused depolarization of the plasma membrane (PM) potential in parenchymal cells. The simultaneous treatment of maize coleoptile segments by indole-3-acetic acid (IAA) and Cd, counteracted the toxic effect of Cd on growth. Moreover, our data also showed that 100 μM Cd suppressed the characteristic IAA-induced hyperpolarization of the membrane potential, causing membrane depolarization. These results indicate that the toxic effect of Cd on growth of maize coleoptile segments might be, at least in part, caused via reduced PM H+-ATPase activity. © 2007 Institute of Experimental Botany, ASCR.},

note = {39},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34250668034,

title = {Effect of temperature on growth, proton extrusion and membrane potential in maize (Zea mays L.) coleoptile segments},

author = { W. Karcz and Z. Burdach},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250668034&doi=10.1007%2fs10725-007-9184-0&partnerID=40&md5=9119ad97aaab51c5ae2b8cf60e2dd54f},

doi = {10.1007/s10725-007-9184-0},

issn = {01676903},

year  = {2007},

date = {2007-01-01},

journal = {Plant Growth Regulation},

volume = {52},

number = {2},

pages = {141-150},

abstract = {The effects of temperature (5-45°C) on endogenous growth, growth in the presence of either indoleacetic acid (IAA) or fusicoccin (FC), and proton extrusion in maize coleoptile segments were studied. In addition, membrane potential changes at some temperatures were also determined. It was found that in this model system endogenous growth exhibits a clear maximum at 30°C, whereas growth in the presence of IAA and FC shows the maximum value in the range 30-35°C and 35-40°C, respectively. Simultaneous measurements of growth and external medium pH indicated that FC at stressful temperatures was not only much more active in the stimulation of growth, but was also more effective in acidifying the external medium than IAA. Also the addition of either IAA or FC to the bathing medium at 30 and 40°C did not change the kinetic characteristic of membrane potential changes observed for both substances at 25°C. However, the increased temperature significantly decreased IAA and FC-induced membrane hyperpolarization. IAA in the incubation medium, at 10°C, brought about additional membrane depolarization (apart from the one induced by low temperature). In contrast to IAA, FC at 10°C caused gradual repolarization of membrane potential, which correlated with both FC-induced growth and FC-induced proton extrusion. A plausible interpretation for temperature-induced changes in growth of maize coleoptile segments is that, at least in part, these changes were mediated via a PM H+-ATPase activity. © 2007 Springer Science+Business Media B.V.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-34247473954,

title = {Quinone-induced inhibition of urease: Elucidation of its mechanisms by probing thiol groups of the enzyme},

author = { W. Zaborska and B. Krajewska and M. Kot and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34247473954&doi=10.1016%2fj.bioorg.2006.11.001&partnerID=40&md5=e10214f86111b099bd03d79f3dbb008f},

doi = {10.1016/j.bioorg.2006.11.001},

issn = {00452068},

year  = {2007},

date = {2007-01-01},

journal = {Bioorganic Chemistry},

volume = {35},

number = {3},

pages = {233-242},

abstract = {In this work we studied the reaction of four quinones, 1,4-benzoquinone (1;4-BQ), 2,5-dimethyl-1,4-benzoquinone (2;5-DM-1;4-BQ), tetrachloro-1,4-benzoquinone (TC-1;4-BQ) and 1,4-naphthoquinone (1;4-NQ) with jack bean urease in phosphate buffer, pH 7.8. The enzyme was allowed to react with different concentrations of the quinones during different incubation times in aerobic conditions. Upon incubation the samples had their residual activities assayed and their thiol content titrated. The titration carried out with use of 5,5′-di-thiobis(2-nitrobenzoic) acid was done to examine the involvement of urease thiol groups in the quinone-induced inhibition. The quinones under investigation showed two distinct patterns of behaviour, one by 1,4-BQ, 2,5-DM-1,4-BQ and TC-1,4-BQ, and the other by 1,4-NQ. The former consisted of a concentration-dependent inactivation of urease where the enzyme-inhibitor equilibrium was achieved in no longer than 10 min, and of the residual activity of the enzyme being linearly correlated with the number of modified thiols in urease. We concluded that arylation of the thiols in urease by these quinones resulting in conformational changes in the enzyme molecule is responsible for the inhibition. The other pattern of behaviour observed for 1,4-NQ consisted of time- and concentration-dependent inactivation of urease with a nonlinear residual activity-modified thiols dependence. This suggests that in 1,4-NQ inhibition, in addition to the arylation of thiols, operative are other reactions, most likely oxidations of thiols provoked by 1,4-NQ-catalyzed redox cycling. In terms of the inhibitory strength, the quinones studied formed a series: 1,4-NQ ≈ 2,5-DM-1,4-BQ < 1,4-BQ < TC-1,4-BQ. © 2006 Elsevier Inc. All rights reserved.},

note = {60},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33750942477,

title = {Nuclear multifragmentation and fission: Similarity and differences},

author = { V.A. Karnaukhov and S.P. Avdeyev and A.S. Botvina and A. Budzanowski and L.V. Chulkov and B. Czech and W. Karcz and V.V. Kirakosyan and E.A. Kuzmin and E. Norbeck and H. Oeschler and V.K. Rodionov and P.A. Rukoyatkin and A.V. Simonenko and I. Skwirczyńska},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33750942477&doi=10.1016%2fj.nuclphysa.2006.09.017&partnerID=40&md5=1bbd0faae3eecb42bae5a24b5d23bf45},

doi = {10.1016/j.nuclphysa.2006.09.017},

issn = {03759474},

year  = {2006},

date = {2006-01-01},

journal = {Nuclear Physics A},

volume = {780},

number = {3-4},

pages = {91-99},

abstract = {Thermal multifragmentation of hot nuclei is interpreted as the nuclear liquid-fog phase transition inside the spinodal region. The experimental data for p (8.1 GeV) + Au collisions are analyzed. It is concluded that the decay process of hot nuclei is characterized by two size parameters: one density at the transition state and one at the kinetic freeze-out. The similarity between the dynamics of fragmentation and ordinary fission is discussed. The IMF emission time is related to the mean rupture time at the multiscission point, which corresponds to the kinetic freeze-out configuration. © 2006 Elsevier B.V. All rights reserved.},

note = {15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33746401517,

title = {Liquid-fog and liquid-gas phase transitions in hot nuclei},

author = { V.A. Karnaukhov and H. Oeschler and A. Budzanowski and S.P. Avdeyev and V.V. Kirakosyan and V.K. Rodionov and P.A. Rukoyatkin and A.V. Simonenko and W. Karcz and I. Skwirczyńska and E.A. Kuzmin and E. Norbeck and A.S. Botvina},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33746401517&doi=10.1134%2fS1063778806070076&partnerID=40&md5=6eb5c2fa997a1d4f6bfdef8d98d25a9e},

doi = {10.1134/S1063778806070076},

issn = {10637788},

year  = {2006},

date = {2006-01-01},

journal = {Physics of Atomic Nuclei},

volume = {69},

number = {7},

pages = {1142-1148},

abstract = {Thermal multifragmentation of hot nuclei is interpreted as the nuclear liquid-fog phase transition inside the spinodal region. The exclusive data for p(8.1 GeV) + Au collisions are analyzed within the framework of the statistical model SMM. It is found that the partition of hot nuclei is specified after expansion to a volume equal to V t = (2.6 ± 0.3)V 0. The freeze-out volume is found to be twice as large: V f = (5 ± 1)V 0. The similarity between multifragmentation and ordinary fission is discussed. © Pleiades Publishing, Inc., 2006.},

note = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2-s2.0-33749334664,

title = {The effect of auxins (IAA and 4-CL-IAA) on the redox activity and medium pH of Zea mays L. root segments},

author = { H. Lekacz and W. Karcz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749334664&doi=10.2478%2fs11658-006-0031-5&partnerID=40&md5=479d95ba2868cac3272e7c662d10b3cf},

doi = {10.2478/s11658-006-0031-5},

issn = {14258153},

year  = {2006},

date = {2006-01-01},

journal = {Cellular and Molecular Biology Letters},

volume = {11},

number = {3},

pages = {376-383},

publisher = {BioMed Central Ltd.},

abstract = {Indole-3-acetic acid (IAA) and 4-chloroindole-3-acetic acid (4-Cl-IAA) were tested at different concentrations and times for their capacity to change the redox activity and medium pH of maize root segments. The dose-response surfaces (dose-response curves as a function of time) plotted for redox activity and changes in medium pH (expressed as ΔpH) had a similar shape for both auxins, but differed significantly at the optimal concentrations. With 4-Cl-IAA, the maximal values of redox activity and medium pH changes were observed at 10-10 M, which was a 100-fold lower concentration than with IAA. Correlations were observed between redox activity and medium pH changes at the optimal concentrations of both IAA and 4-Cl-IAA. The results are discussed herein, taking into account both the concentration of the auxins and the effects produced by them. © 2006 by the University of Wrocław.},

note = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}