2018
Joachimiak, A. J.; Hasterok, R.; Sliwinska, E.; Musiał, K.; Grabowska-Joachimiak, A.
FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa Journal Article
In: Protoplasma, vol. 255, no. 5, pp. 1363-1372, 2018, ISSN: 0033183X, (6).
@article{2-s2.0-85043690137,
title = {FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa},
author = { A.J. Joachimiak and R. Hasterok and E. Sliwinska and K. Musiał and A. Grabowska-Joachimiak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043690137&doi=10.1007%2fs00709-018-1238-9&partnerID=40&md5=6d557cd07897efaa2df1b3fd9cc4b72d},
doi = {10.1007/s00709-018-1238-9},
issn = {0033183X},
year = {2018},
date = {2018-01-01},
journal = {Protoplasma},
volume = {255},
number = {5},
pages = {1363-1372},
publisher = {Springer-Verlag Wien},
abstract = {The location of 5S and 35S rDNA sequences in chromosomes of four Aconitum subsp. Aconitum species was analyzed after fluorescence in situ hybridization (FISH). Both in diploids (2n = 2x = 16; Aconitum variegatum; A. degenii) and tetraploids (2n = 4× = 32; A. firmum; A. plicatum), rDNA repeats were localized exclusively on the shorter arms of chromosomes, in subterminal or pericentromeric sites. All analyzed species showed similar basal genome size (Cx = 5.31–5.71 pg). The most striking features of tetraploid karyotypes were the conservation of diploid rDNA loci and emergence of many additional 5S rDNA clusters. Chromosomal distribution of excessive ribosomal sites suggests their role in the secondary diploidization of tetraploid karyotypes. © 2018, The Author(s).},
note = {6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The location of 5S and 35S rDNA sequences in chromosomes of four Aconitum subsp. Aconitum species was analyzed after fluorescence in situ hybridization (FISH). Both in diploids (2n = 2x = 16; Aconitum variegatum; A. degenii) and tetraploids (2n = 4× = 32; A. firmum; A. plicatum), rDNA repeats were localized exclusively on the shorter arms of chromosomes, in subterminal or pericentromeric sites. All analyzed species showed similar basal genome size (Cx = 5.31–5.71 pg). The most striking features of tetraploid karyotypes were the conservation of diploid rDNA loci and emergence of many additional 5S rDNA clusters. Chromosomal distribution of excessive ribosomal sites suggests their role in the secondary diploidization of tetraploid karyotypes. © 2018, The Author(s).
2008
Golczyk, H.; Joachimiak, A. J.; Hasterok, R.
Pericentromeric gc-rich chromatin in rhoeo (commelinaceae). Evidence from soma and germ-line Journal Article
In: Caryologia, vol. 61, no. 4, pp. 388-391, 2008, ISSN: 00087114, (4).
@article{2-s2.0-70249083748,
title = {Pericentromeric gc-rich chromatin in rhoeo (commelinaceae). Evidence from soma and germ-line},
author = { H. Golczyk and A.J. Joachimiak and R. Hasterok},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-70249083748&doi=10.1080%2f00087114.2008.10589651&partnerID=40&md5=3985e23aa885dc9b09e6390770fdca61},
doi = {10.1080/00087114.2008.10589651},
issn = {00087114},
year = {2008},
date = {2008-01-01},
journal = {Caryologia},
volume = {61},
number = {4},
pages = {388-391},
abstract = {— CMA/DA/DAPI technique revealed the presence of GC-rich regions in mitotic/meiotic chromosomes and within meiotic collective chromocenters of permanent heterozygote Rhoeo spathacea (2n = 12). CMA-bands were detected within some NOR-related chromosome ends and within all twelve pericentromeric regions. It can not be excluded that GC-rich segments associated with AT-rich pericentromeric heterochromatin may serve as landmarks of the putative translocation breakpoints in this species. The persistence of two cytologically well-defned chromatin domains (AT and GC-rich) within pericentromeres of all chromosomes suggests spreading and homogenization of their sequences during evolution of Rhoeo karyotype. © 2008 Taylor & Francis Group, LLC.},
note = {4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
— CMA/DA/DAPI technique revealed the presence of GC-rich regions in mitotic/meiotic chromosomes and within meiotic collective chromocenters of permanent heterozygote Rhoeo spathacea (2n = 12). CMA-bands were detected within some NOR-related chromosome ends and within all twelve pericentromeric regions. It can not be excluded that GC-rich segments associated with AT-rich pericentromeric heterochromatin may serve as landmarks of the putative translocation breakpoints in this species. The persistence of two cytologically well-defned chromatin domains (AT and GC-rich) within pericentromeres of all chromosomes suggests spreading and homogenization of their sequences during evolution of Rhoeo karyotype. © 2008 Taylor & Francis Group, LLC.
2005
Golczyk, H.; Hasterok, R.; Joachimiak, A. J.
FISH-aimed karyotyping and characterization of Renner complexes in permanent heterozygote Rhoeo spathacea Journal Article
In: Genome, vol. 48, no. 1, pp. 145-153, 2005, ISSN: 08312796, (25).
@article{2-s2.0-19944402530,
title = {FISH-aimed karyotyping and characterization of Renner complexes in permanent heterozygote Rhoeo spathacea},
author = { H. Golczyk and R. Hasterok and A.J. Joachimiak},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-19944402530&doi=10.1139%2fg04-093&partnerID=40&md5=6b77a7b69dd27d2277e15ab56eded9cf},
doi = {10.1139/g04-093},
issn = {08312796},
year = {2005},
date = {2005-01-01},
journal = {Genome},
volume = {48},
number = {1},
pages = {145-153},
abstract = {Fluorescence in situ hybridization (FISH) using 25S rDNA, 5S rDNA, and telomere sequences as probes was carried out in the complex permanent heterozygote Rhoeo spathacea. Telomere sites were exclusively terminal. All 10 25S rDNA loci were located distally and appeared transcriptionally active after silver staining. Six distal and 2 interstitial 5S rDNA sites were detected; 2 of the distal sites strictly colocalized with 25S rDNA loci. The 2 intercalary 5S rDNA loci occurred in short arms of 2 chromosomes that conjoined at meiosis. Chromosomes differed as to the amount of AT-rich centric heterochromatin, suggesting involvement of pericentromeric regions in translocations. The possibility of Robertsonian-like rearrangements was discussed. Double target FISH with ribosomal probes along with DAPI fluorescence gave the basis for full chromosome identification in mitosis. The 2 Renner complexes are structurally balanced, both having 5 25S and 4 5S rDNA sites. Centromere clustering, telomere association, a high number of NOR sites, and a strong tendency for formation of joint nucleoli contribute to the preservation of highly polarized Rabl arrangement at interphase. These findings were discussed in relation to meiotic catenation in Rhoeo. © 2005 NRC Canada.},
note = {25},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Fluorescence in situ hybridization (FISH) using 25S rDNA, 5S rDNA, and telomere sequences as probes was carried out in the complex permanent heterozygote Rhoeo spathacea. Telomere sites were exclusively terminal. All 10 25S rDNA loci were located distally and appeared transcriptionally active after silver staining. Six distal and 2 interstitial 5S rDNA sites were detected; 2 of the distal sites strictly colocalized with 25S rDNA loci. The 2 intercalary 5S rDNA loci occurred in short arms of 2 chromosomes that conjoined at meiosis. Chromosomes differed as to the amount of AT-rich centric heterochromatin, suggesting involvement of pericentromeric regions in translocations. The possibility of Robertsonian-like rearrangements was discussed. Double target FISH with ribosomal probes along with DAPI fluorescence gave the basis for full chromosome identification in mitosis. The 2 Renner complexes are structurally balanced, both having 5 25S and 4 5S rDNA sites. Centromere clustering, telomere association, a high number of NOR sites, and a strong tendency for formation of joint nucleoli contribute to the preservation of highly polarized Rabl arrangement at interphase. These findings were discussed in relation to meiotic catenation in Rhoeo. © 2005 NRC Canada.