Radoń, A.; Deregowska, A.; Ciuraszkiewicz, A.; Hawełek, Ł.; Spałek, H.; Warski, T.; Hudecki, A.; Łukowiec, D.; Piotrowski, P.; Krogul-Sobczak, A.; Rost-Roszkowska, M. M.; Chajec, Ł.; Pieszko, W.; Bukala, J.; Rzeszutek, J.; Wnuk, M.; Lewińska, A. In: ACS Biomaterials Science and Engineering, vol. 12, no. 5, pp. 2619-2635, 2026, (0). Ziętara-Krzyk, P.; Flasz, B.; Augustyniak, M. Effects of resveratrol and nanodiamonds on sirtuin activity, oxidative stress and DNA damage in Acheta domesticus Journal Article In: Biochemical and Biophysical Research Communications, vol. 816, 2026, (1). Gorol, H.; Chajec, Ł.; Wilczek, G.; Student, S.; Ostróżka, A.; Rost-Roszkowska, M. M. Bisphenol A disrupts epithelial homeostasis in the midgut of the freshwater shrimp Neocaridina davidi Journal Article In: European Zoological Journal, vol. 93, no. 1, pp. 563-582, 2026, (0). Ahmed, M. I.; Nowak, A.; Dulski, M.; Strach, A.; Zielińska, A.; Paul-Samojedny, M.; Potocka, I. W.; Matus, K.; Wasilkowski, D. In: Materials, vol. 19, no. 8, 2026, (0). Pietruszka, M. A. Temperature-dependent bistable electrical switching in hydrated barley genomic DNA Journal Article In: BioSystems, vol. 263, 2026, (0). Ziętara-Krzyk, P.; Flasz, B.; Augustyniak, M. Evaluation of Molecular Responses and Longevity Markers in Acheta domesticus Following Combined Resveratrol and Nanodiamond Exposure Journal Article In: International Journal of Molecular Sciences, vol. 27, no. 6, 2026, (0). Cieplok, A.; Orpych, B.; Chrobak, K.; Rusinek, K.; Czerniawski, R.; Spyra, A. Evaluation of non-opioid analgesic toxicity and their effects on native and invasive species of crustaceans (Gammaridae, Gammarus) Journal Article In: BioRisk, no. 24, pp. 85-102, 2026, (0). Yücel, G.; Skinderowicz, R.; Demir, O.; Kolano, B. A.; Tuna, M. Nuclear DNA Content Analyses by Flow Cytometry of Saffron (Crocus sativus L.) Populations Obtained from Safranbolu, Türkiye Journal Article In: Current Issues in Molecular Biology, vol. 48, no. 3, 2026, (0). Musielak, M.; Serda, M.; Chavhan, M. P.; Małecka, M. A.; Chajec, Ł.; Małota, K.; Szubka, M.; Rost-Roszkowska, M. M.; Sitko, R. Cr(VI) detoxification from water using a regenerable fullerene-based hybrid adsorbent with in vivo safety assessment Journal Article In: Water Resources and Industry, vol. 35, 2026, (0). Flasz, B.; Babczyńska, A.; Tarnawska, M.; Ajay, A. K.; Kędziorski, A.; Napora-Rutkowski, Ł.; Świerczek, E.; Rozpędek, K.; Augustyniak, M. Response to multigenerational graphene oxide exposure in acheta domesticus strains selected for longevity Journal Article In: Scientific Reports, vol. 16, no. 1, 2026, (0). Battaglino, C.; Bodnaruk, I.; Czyszczoń, P.; Skurnik, M.; Filip-Psurska, B.; Jarych, D.; Maciejewska, A.; Gadzinowski, M.; Malik, K.; Potocka, I. W.; Migdał, P.; Kruszakin, R.; Cedzyński, M.; Kasperkiewicz, K.; Łukasiewicz, J.; Świerzko, A. S. Yersinia enterocolitica O:3 Outer Membrane Vesicles as a Platform for Complement Activation Journal Article In: Journal of Extracellular Vesicles, vol. 15, no. 4, 2026, (0). Wieczorek, K.; Chłond, D.; Ball, K.; Zawisza-Raszka, A.; Zielińska, A.; Baert, D.; Elliot, M. In: Insects, vol. 17, no. 2, 2026, (0). Nowak, A.; Wojcieszyńska, D.; Pacwa-Płociniczak, M.; Guzik, U. A synergistic-like interaction of phenol and iohexol on the structure and functioning of the activated sludge microbiome Journal Article In: International Biodeterioration and Biodegradation, vol. 212, 2026, (0). Noszczyńska, M.; Mitera, M.; Zając, G.; Rudnicka, M.; Pacwa-Płociniczak, M.; Nycz, A.; Wojtkowska, M.; Przybyla-Kasperek, M.; Skinderowicz, R.; Płociniczak, T. In: International Biodeterioration and Biodegradation, vol. 212, 2026, (0). Kwiatkowska, D.; Borowska-Wykręt, D. Auxin–cytokinin crosstalk and differential growth: complex relationships driving carpel initiation Journal Article In: Journal of Experimental Botany, vol. 77, no. 6, pp. 1520-1524, 2026, (0). Wójcikowska, B. The role of nitrilases in auxin biosynthesis, plant development and stress responses Journal Article In: Plant and Cell Physiology, vol. 67, no. 2, pp. 115-129, 2026, (1). El-Aradi, A.; Nowak, A.; Kasim, T.; Webb, D. J.; Nowakowski, D. J. Advancing soil health with biochar - Effects on soil microbial activity and diversity Journal Article In: Science of the Total Environment, vol. 1031, 2026, (0). Karczmarzyk, A.; Wojcieszyńska, D.; Nowak, A.; Smułek, W.; Guzik, U. Berberine Toxicity Profile in Experimental Models as a Basis for Assessing Its Biological Safety Journal Article In: Molecules, vol. 31, no. 8, 2026, (0). Piekarz, S.; Płociniczak, T.; Noszczyńska, M. Microbial Transformation of Agriculture-Related Microplastics in Aquatic Environments Journal Article In: Agriculture (Switzerland), vol. 16, no. 9, 2026, (0). Gaj, M. D. The transcription factor LEC2 as an epigenetic regulator of plant totipotency: from Arabidopsis to crop improvement Journal Article In: Journal of Applied Genetics, 2026, (0).@article{2-s2.0-105038435739,
title = {Fabrication of Electrospun PCL/PEO Microfibers with Fe3O4 Nanoparticles for Magnetic Hyperthermia: Immunocompatibility Assessment Using CD14+ Monocytes, CD4+ and CD8+ T Cells, and CD56+ NK Cells In Vitro},
author = { A. Radoń and A. Deregowska and A. Ciuraszkiewicz and Ł. Hawełek and H. Spałek and T. Warski and A. Hudecki and D. Łukowiec and P. Piotrowski and A. Krogul-Sobczak and M.M. Rost-Roszkowska and Ł. Chajec and W. Pieszko and J. Bukala and J. Rzeszutek and M. Wnuk and A. Lewińska},
url = {https://www.scopus.com/pages/publications/105038435739?origin=resultslist},
doi = {10.1021/acsbiomaterials.5c02218},
year = {2026},
date = {2026-01-01},
journal = {ACS Biomaterials Science and Engineering},
volume = {12},
number = {5},
pages = {2619-2635},
publisher = {American Chemical Society},
abstract = {Magnetite nanoparticles (Fe3O4 NPs), due to their unique physicochemical properties, are considered as promising nanomaterials for multiple biomedical applications. However, the development of novel strategies for surface modification and coating of Fe3O4 NPs is needed to fabricate Fe3O4 NPs with improved biocompatibility. In the present study, two polymers, namely, poly(ε-caprolactone) (PCL) and poly(ethylene oxide) (PEO), were applied to produce PCL/PEO microfibers containing Fe3O4 NPs (PCL/PEO/Fe3O4 MFs) using the electrospinning method. Their physicochemical properties, especially magnetically induced hyperthermia effects, were compared to Fe3O4 NPs. The biocompatibility and immunocompatibility of PCL/PEO/Fe3O4 MFs were then tested using four types of human immune cells, namely, CD14+ monocytes, CD4+ helper, CD8+ cytotoxic T cells, and CD56+ NK cells. Monocytes were the most sensitive to PCL/PEO/Fe3O4 MFs as judged by the induction of cell death (apoptosis and necrosis) and micronuclei production, whereas other immune cells were less or not affected by the stimulation with PCL/PEO/Fe3O4 MFs. PCL/PEO/Fe3O4 MFs also did not lower the viability of normal human fibroblasts. Furthermore, a mild immunogenic response was revealed in PCL/PEO/Fe3O4 MF-treated helper T cells based on the analysis of transcriptional activity of 92 genes involved in the NFκB pathway. Observed elevated mRNA levels of NFKB2, TNF, TNFAIP3, TRAF1, and TBK1 may have context-dependent immunomodulatory effects in PCL/PEO/Fe3O4 MF-stimulated helper T cells that should be taken into account while designing novel drug-delivery systems based on PCL/PEO and Fe3O4 NPs. © 2026 American Chemical Society},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105034668070,
title = {Effects of resveratrol and nanodiamonds on sirtuin activity, oxidative stress and DNA damage in Acheta domesticus},
author = { P. Ziętara-Krzyk and B. Flasz and M. Augustyniak},
url = {https://www.scopus.com/pages/publications/105034668070?origin=resultslist},
doi = {10.1016/j.bbrc.2026.153698},
year = {2026},
date = {2026-01-01},
journal = {Biochemical and Biophysical Research Communications},
volume = {816},
publisher = {Elsevier B.V.},
abstract = {The quest for an elixir of longevity has long inspired research into molecular mechanisms that govern aging. The present study investigated the effects of resveratrol (RV) and nanodiamonds (NDs) on sirtuin activity, oxidative stress, and DNA damage in two strains of Acheta domesticus: wild-type (H) and longevity-selected (D). Analyses of total SIRT and SIRT1, SIRT6 activities, antioxidant markers (CAT; SOD; LPO), and DNA damage indicators (pATM; γH2A.X; DSBs) revealed strain- and stage-dependent variations without a consistent pattern, suggesting long-term modulation of lifespan or sirtuin activity. RV and NDs induced only transient and adaptive effects, including a short-term increase in sirtuin activity exposed to NDs. The long-lived strain displayed stability in sirtuin response and survival, indicating that longevity-regulating mechanisms are genetically conserved and resistant to external modulation. These findings offer new insights into the limited and context-dependent influence of nanomaterials and bioactive compounds on aging-related molecular pathways. © 2026 Elsevier Inc.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105035915051,
title = {Bisphenol A disrupts epithelial homeostasis in the midgut of the freshwater shrimp Neocaridina davidi},
author = { H. Gorol and Ł. Chajec and G. Wilczek and S. Student and A. Ostróżka and M.M. Rost-Roszkowska},
url = {https://www.scopus.com/pages/publications/105035915051?origin=resultslist},
doi = {10.1080/24750263.2026.2652113},
year = {2026},
date = {2026-01-01},
journal = {European Zoological Journal},
volume = {93},
number = {1},
pages = {563-582},
publisher = {Taylor and Francis Ltd.},
abstract = {Bisphenol A (BPA) is commonly used in the production of plastics and epoxy resins. Hence, it is used to produce many everyday items. It is classified as a hazardous chemical due to its potential to cause serious eye damage, allergic skin reactions, and respiratory irritation or can affect reproductive function, cognitive function, and metabolism. Furthermore, BPA, due to its ability to mimic the human hormone estrogen, is an endocrine disruptor. However, aquatic organisms are most vulnerable to BPA. While changes in the abundance and fecundity of freshwater invertebrates have been analysed, data on the cytotoxic effects of BPA are scarce. Cytotoxicity triggers numerous degenerative or regenerative processes that attempt to maintain homeostasis in the body. Thus, to determine which cytotoxicological processes could be activated after BPA exposure, we used the freshwater shrimp Neocaridina davidi, a species widely cultured worldwide. Individuals of N. davidi were exposed to three concentrations of BPA: 1, 5, and 10 mg/L for 24, 48, and 72 h. Isolated from the specimens, the intestine and hepatopancreas were prepared for qualitative and quantitative analysis. The present findings demonstrate that BPA disrupts multiple cytotoxicity pathways, triggering a cascade of cellular interactions, including damage to mitochondria, nuclei, and DNA. We conclude that the oxidant-antioxidant imbalance induced by BPA compromises epithelial cell homeostasis, leading to midgut barrier dysfunction and impairing the regenerative capacity of the digestive system. © 2026 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105040669267,
title = {Metal Oxide Nanocomposites as Next-Generation Antimicrobial Agents Against Oral Cariogenic Pathogens: Mechanistic Actions of Ag–ZnO and Cu–ZnO on S. mutans and S. sobrinus},
author = { M.I. Ahmed and A. Nowak and M. Dulski and A. Strach and A. Zielińska and M. Paul-Samojedny and I.W. Potocka and K. Matus and D. Wasilkowski},
url = {https://www.scopus.com/pages/publications/105040669267?origin=resultslist},
doi = {10.3390/ma19081634},
year = {2026},
date = {2026-01-01},
journal = {Materials},
volume = {19},
number = {8},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {Oral infections caused by antibiotic-resistant bacteria represent an emerging biomedical hazard and growing challenge for modern dentistry. To address this issue, Ag– and Cu–ZnO nanocomposites (NCs) were synthesized using ZnO carrier to combat the oral pathogens Streptococcus mutans and Streptococcus sobrinus. A comprehensive analysis of chemically synthesized metal oxide nanocomposites (MONCs) was performed, combining physicochemical characterization (TEM; XRD; ζ-potential; DLS; pH; and PFO/PSO kinetic models) with biological toxicity assessment (MIC; ATR–FTIR; SEM; and FAMEs) to better understand their antimicrobial mechanisms. The results confirmed that the synthesized nanoproducts fulfill the criteria for nanomaterials (NMs) (particle size < 100 nm). Among them, Ag–ZnO exhibited the highest antibacterial activity against both strains (MIC = 50 mg L−1). Kinetic modeling revealed faster and more efficient Ag ion release from Ag–ZnO NCs compared to Cu from Cu–ZnO NCs. Molecular analyses indicated strong MONC–bacterial interactions at the cell surface, leading to changes in protein secondary structures, alterations in lipid composition, and disruption of Gram-positive bacterial membranes. Additionally, Ag–ZnO inhibited chain and cluster formation in both bacterial species, while Cu–ZnO affected only S. sobrinus. Overall, Ag– and Cu–ZnO NCs show strong potential as antimicrobial agents against oral pathogens. © 2026 by the authors.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105033605438,
title = {Temperature-dependent bistable electrical switching in hydrated barley genomic DNA},
author = { M.A. Pietruszka},
url = {https://www.scopus.com/pages/publications/105033605438?origin=resultslist},
doi = {10.1016/j.biosystems.2026.105777},
year = {2026},
date = {2026-01-01},
journal = {BioSystems},
volume = {263},
publisher = {Elsevier Ireland Ltd},
abstract = {Hydrated genomic DNA exhibits complex collective behavior mediated by its proton-rich hydrogen-bond network. Here, we report temperature-dependent bistable switching in hydrated barley genomic DNA under a weak electrical bias and a moderate magnetic field (0.5 T). The hydrated sample was prepared in a mixed DNA–saline environment at approximately a 1:1 volume ratio (50/50) with physiological ionic strength (0.9% NaCl), providing controlled ionic screening conditions. After normalizing the transverse response by the longitudinal current to remove drive drift, the system displays telegraph-like switching between two preferred macroscopic states during cooling at a fixed magnetic field. The switching rate and dwell-time statistics vary strongly with temperature, with characteristic changes occurring near 18 °C and 14 °C to 15 °C, temperature ranges previously associated with functional hydration regimes in barley DNA. These results demonstrate that bistable collective electrical dynamics persist under physiologically relevant ionic conditions, indicating robust temperature-tuned electrical behavior of hydrated genomic DNA under ambient conditions. Copyright © 2026. Published by Elsevier B.V.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105034168058,
title = {Evaluation of Molecular Responses and Longevity Markers in Acheta domesticus Following Combined Resveratrol and Nanodiamond Exposure},
author = { P. Ziętara-Krzyk and B. Flasz and M. Augustyniak},
url = {https://www.scopus.com/pages/publications/105034168058?origin=resultslist},
doi = {10.3390/ijms27062786},
year = {2026},
date = {2026-01-01},
journal = {International Journal of Molecular Sciences},
volume = {27},
number = {6},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {Sirtuins are conserved proteins regulating oxidative stress and lifespan. While they enhance cellular adaptability, the long-term biological consequences of combining bioactive compounds with nanomaterials remain poorly understood. This study examined the effects of combined resveratrol and nanodiamonds (RV+NDs) in two Acheta domesticus strains: wild-type (H) and longevity-selected (D). The impact was assessed across developmental stages, focusing on survival, total sirtuin activity, specific isoforms (SIRT1; SIRT6), oxidative stress, antioxidant enzymes, and DNA damage markers. RV+NDs exposure did not result in consistent lifespan extension or sustained oxidative stress. Molecular responses were strongly dictated by genetic background and age, as reflected by significant survival differences between strains H and D (p < 0.001) Notably, a persistent increase in total sirtuin activity (~60% ↑ across developmental stages) occurred exclusively in the longevity-selected strain, though no stable activation of SIRT1 or SIRT6 was detected. While classical redox parameters showed only transient changes, DNA damage response markers emerged as the most sensitive indicators of RV+NDs exposure. Overall, the findings demonstrate that RV+NDs treatment induces context-dependent, adaptive molecular responses. This highlights the critical role of genetic background and age in shaping ageing-related pathways, suggesting that nanodelivery systems do not produce universal effects across different genotypes. © 2026 by the authors.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105036415373,
title = {Evaluation of non-opioid analgesic toxicity and their effects on native and invasive species of crustaceans (Gammaridae, Gammarus)},
author = { A. Cieplok and B. Orpych and K. Chrobak and K. Rusinek and R. Czerniawski and A. Spyra},
url = {https://www.scopus.com/pages/publications/105036415373?origin=resultslist},
doi = {10.3897/biorisk.24.176556},
year = {2026},
date = {2026-01-01},
journal = {BioRisk},
number = {24},
pages = {85-102},
publisher = {Pensoft Publishers},
abstract = {Humans widely use pharmaceuticals due to their availability, effectiveness, quick pain relief, and ability to improve well-being and health conditions. Their presence in aquatic environments, even in small doses, leads to serious ecological disturbances and alterations in trophic chains. This study aimed to assess the mortality and mobility of native Gammarus pulex and invasive Gammarus tigrinus under exposure to the nonopioid analgesics paracetamol, ibuprofen, ketoprofen, and a pharmaceutical mixture, as well as to determine their acute toxicity and impact on both species. At the 24th and 96th h of exposure, the highest increase in mortality was observed, most likely linked to shock caused by the sudden appearance of pollutants and the duration of exposure. Mortality was influenced not only by exposure time but also by the type of pharmaceutical. Paracetamol was the most lethal for both native and invasive species, though 100% mortality was recorded after 96 h of exposure. At a concentration of 5000 mg/L, all organisms died within 24 h. G. tigrinus, despite its broader ecological tolerance to pollution and resistance to environmental changes, responded to the presence of pharmaceuticals like G. pulex. Regarding survival, the ibuprofen–ketoprofen mixture in a 1:1 ratio was the least lethal for G. tigrinus, while ketoprofen alone was the least lethal for G. pulex. The availability and widespread use of over-the-counter pharmaceuticals, combined with the lack of systematic monitoring of aquatic environments, pose serious challenges to aquatic organisms in the context of pharmaceutical pollution in water habitats. Copyright: © Anna Cieplok et al. This is an open access article distributed under terms of the Creative Commons Attribution License (Attribution 4.0 International - CC BY 4.0).},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105034399978,
title = {Nuclear DNA Content Analyses by Flow Cytometry of Saffron (Crocus sativus L.) Populations Obtained from Safranbolu, Türkiye},
author = { G. Yücel and R. Skinderowicz and O. Demir and B.A. Kolano and M. Tuna},
url = {https://www.scopus.com/pages/publications/105034399978?origin=resultslist},
doi = {10.3390/cimb48030262},
year = {2026},
date = {2026-01-01},
journal = {Current Issues in Molecular Biology},
volume = {48},
number = {3},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {C. sativus (saffron) is the source of the world’s most expensive spice. Despite its economic significance, the genome structure is poorly studied. C. sativus is a sterile triploid (2n = 3x = 24) species, traditionally considered to exhibit minimal genetic variation. In this study, we analysed 45 individuals representing 15 accessions of C. sativus obtained from farmers in the Davutobası and Yukarıçiftlik villages of Safranbolu—an important centre of saffron cultivation in Türkiye. These populations represent an underexplored reservoir of germplasm with potential implications for biodiversity, conservation, and genetic improvement. Flow cytometry based on propidium iodide staining was used to assess nuclear DNA content, a key cytogenetic characteristic relevant to taxonomy, breeding, and molecular research. Nuclear DNA content among individuals ranged from 10.45 pg/2C DNA to 10.9 pg/2C DNA, all sharing the expected triploid chromosome number (2n = 3x = 24). Although variation was subtle, the observed polymorphism suggests the presence of detectable genomic diversity within these genotypes. These findings highlight the importance of analysing genotypes in understanding the genetic landscape of C. sativus. Selected individuals exhibiting variation in genome size may serve as valuable material for further molecular and breeding studies aimed at improving this culturally and economically significant crop. © 2026 by the authors.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105037093423,
title = {Cr(VI) detoxification from water using a regenerable fullerene-based hybrid adsorbent with in vivo safety assessment},
author = { M. Musielak and M. Serda and M.P. Chavhan and M.A. Małecka and Ł. Chajec and K. Małota and M. Szubka and M.M. Rost-Roszkowska and R. Sitko},
url = {https://www.scopus.com/pages/publications/105037093423?origin=resultslist},
doi = {10.1016/j.wri.2026.100366},
year = {2026},
date = {2026-01-01},
journal = {Water Resources and Industry},
volume = {35},
publisher = {Elsevier B.V.},
abstract = {Hexavalent chromium contamination remains a serious challenge for industrial water and wastewater treatment due to its high toxicity, mobility and resistance to conventional remediation methods. Here we report an adsorption-based detoxification approach using a regenerable hybrid nanoadsorbent (GO-C60-PEI) that integrates graphene oxide, [60]fullerene and branched polyethyleneimine. Microscopy and spectroscopic analyses (SEM/TEM; FT-IR; XPS; CHNS) together with N2–sorption confirm successful construction of the hybrid architecture. Cr(VI) uptake is rapid (equilibrium within 45 min) and best described by a Langmuir monolayer model, giving a maximum capacity of 380 mg g−1 at pH 1.5. The GO-C60-PEI adsorbs quantitative Cr(VI) even under an unfavorable aqueous environment of high ionic strength of the solution (up to 3M of NaNO3), and in the presence of million-fold co-existing ions in relation to Cr(VI). The material can be regenerated within 5 min using 1.5 mL of 2M NH3(aq) assisted by ultrasonication, allowing efficient Cr(VI) recovery. Finally, in vivo toxicological studies using the Drosophila melanogaster model confirmed the absence of toxicity for both the pristine nanomaterial and its Cr(VI)-loaded form, indicating effective detoxification of the treated water. Therefore, the obtained GO-C60-PEI represents a promising and eco-friendly platform for the purification and detoxification of Cr(VI) in water treatment systems. © 2026 The Authors.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105030486320,
title = {Response to multigenerational graphene oxide exposure in acheta domesticus strains selected for longevity},
author = { B. Flasz and A. Babczyńska and M. Tarnawska and A.K. Ajay and A. Kędziorski and Ł. Napora-Rutkowski and E. Świerczek and K. Rozpędek and M. Augustyniak},
url = {https://www.scopus.com/pages/publications/105030486320?origin=resultslist},
doi = {10.1038/s41598-026-37623-7},
year = {2026},
date = {2026-01-01},
journal = {Scientific Reports},
volume = {16},
number = {1},
publisher = {Nature Research},
abstract = {The development of new nanotechnologies and their use in everyday life always carries the risk of environmental hazards and consequences for human health. Among them, graphene oxide (GO) is a promising material. Due to its excellent physicochemical properties, GO is attractive not only for industrial applications but also in medicine. There is still a lack of sufficient reports on the long-term effects of GO on organisms, including studies of a multigenerational nature. We investigated the health status of two strains of Acheta domesticus: the wild type and the long-lived. The strains were exposed to GO for five generations and a sixth recovery generation. We investigated parameters that may indirectly explain the mechanisms involved in transmitting the informational pattern of the stress response to subsequent generations: DNA stability, mitochondrial potential, apoptosis, and autophagy. GO intoxication induced multilevel cellular responses in five subsequent generations. GO cessation in recovery F5 acted as a new stressor. Across five generations, variation in the response to GO was observed. GO is most likely responsible for changes that persist over generations. We believe that epigenetic inheritance is a likely mechanism underlying the multigenerational adaptation observed in GO-exposed insects, and future research should aim to elucidate this phenomenon in more detail. © The Author(s) 2026.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105035872755,
title = {Yersinia enterocolitica O:3 Outer Membrane Vesicles as a Platform for Complement Activation},
author = { C. Battaglino and I. Bodnaruk and P. Czyszczoń and M. Skurnik and B. Filip-Psurska and D. Jarych and A. Maciejewska and M. Gadzinowski and K. Malik and I.W. Potocka and P. Migdał and R. Kruszakin and M. Cedzyński and K. Kasperkiewicz and J. Łukasiewicz and A.S. Świerzko},
url = {https://www.scopus.com/pages/publications/105035872755?origin=resultslist},
doi = {10.1002/jev2.70270},
year = {2026},
date = {2026-01-01},
journal = {Journal of Extracellular Vesicles},
volume = {15},
number = {4},
publisher = {John Wiley and Sons Inc},
abstract = {Lipopolysaccharide (LPS) is the major component of the outer membrane vesicles (OMVs) of Gram-negative bacteria. We hypothesized that OMVs-induced complement activation contributes to the development of systemic inflammatory response syndrome (SIRS). Yersinia enterocolitica O:3 (YeO3) variants synthesizing LPS of various chemotypes (S; Ra; Rd1; Re) were used as model microorganisms. OMVs activated complement more potently than parental bacteria or homologous LPS, independently of chemotype. A high molecular weight polysaccharide fraction, distinct from LPS, was recognized by serum mannose-binding lectin (MBL). In vivo experiments demonstrated that complement depletion weakened the hallmarks of OMVs-induced SIRS in mice. LPS chemotype affected the biodistribution of OMVs and long O-specific polysaccharide protected them from clearance. Chemotype influenced OMVs secretion with their highest release by bacteria with LPS reduced to the inner core and lipid A (Rd1). The shift from environmental to host's temperature stimulated secretion of smaller OMVs, with less-toxic, tetra-acyl lipid A. Our data are consistent with a contribution of OMVs to Yersinia pathogenicity through complement activation. Their potency as complex virulence factor is influenced by size, length of oligo-/polysaccharide chain, and lipid A form. This study comprehensively characterizes OMV-complement interactions in YeO3, extending the knowledge of mechanisms previously established for other Gram-negative bacteria. © 2026 The Author(s). Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105031368064,
title = {Aphids (Hemiptera: Aphididae) in Living Collections of Selected European Botanic Gardens: Diversity, Biosecurity Challenges, and Sentinel Insights},
author = { K. Wieczorek and D. Chłond and K. Ball and A. Zawisza-Raszka and A. Zielińska and D. Baert and M. Elliot},
url = {https://www.scopus.com/pages/publications/105031368064?origin=resultslist},
doi = {10.3390/insects17020196},
year = {2026},
date = {2026-01-01},
journal = {Insects},
volume = {17},
number = {2},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {Botanic gardens host diverse living plant collections and are increasingly recognized as sentinel sites for documenting insect biodiversity and detecting biological invasions. Aphids (Hemiptera: Aphididae) are well suited to such monitoring due to their close host associations, rapid population growth, and importance as horticultural pests and virus vectors. Here, we document the aphid fauna recorded in five European botanic gardens—Zabrze (Poland), Meise (Belgium), and the Royal Botanic Gardens in Edinburgh, Benmore, and Logan (UK)—based on surveys conducted in 2022 and 2023. Sampling approaches included short-duration expert bioblitz-style surveys and extended seasonal monitoring. In total, more than one hundred aphid species were recorded across all sites. Observed species lists differed among gardens and survey periods, reflecting variation in sampling timing, intensity, and host plant composition. Several alien aphid species to Europe of Oriental and Nearctic origin were detected, including multiple new national records. Alien taxa were found both in outdoor living collections and in controlled environments such as glasshouses, nurseries, restricted areas, and plant shops, which may function as entry points as well as locations for early intervention. These findings illustrate the value of botanic gardens for documenting aphid diversity and supporting early detection of non-native species relevant to plant health and biosecurity. © 2026 by the authors.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105037827470,
title = {A synergistic-like interaction of phenol and iohexol on the structure and functioning of the activated sludge microbiome},
author = { A. Nowak and D. Wojcieszyńska and M. Pacwa-Płociniczak and U. Guzik},
url = {https://www.scopus.com/pages/publications/105037827470?origin=resultslist},
doi = {10.1016/j.ibiod.2026.106359},
year = {2026},
date = {2026-01-01},
journal = {International Biodeterioration and Biodegradation},
volume = {212},
publisher = {Elsevier Ltd},
abstract = {The increasing occurrence of pharmaceutical residues and industrial contaminants in wastewater poses a growing challenge for biological treatment systems. Understanding how these compounds interact is essential for predicting their impact on microbial communities and treatment efficiency. Iohexol, a widely used iodinated contrast agent, is regarded as a compound of low toxicity to microorganisms when present alone; however, its impact under co-occurrence with other wastewater contaminants remains poorly understood. Phenol is a ubiquitous component of municipal and industrial wastewater and a well-known microbial substrate, yet its role in modulating microbial responses to co-existing micropollutants has not been sufficiently investigated. This study evaluated the non-additive effects of phenol and iohexol on the microbiome and functional performance of activated sludge from the Klimzowiec wastewater treatment plant (Poland). Although phenol was completely degraded during each reactor cycle, indicating strong microbial adaptation to aromatic compounds, its presence did not improve iohexol biotransformation compared to phenol-free systems. Only a minor improvement in iohexol removal was observed during subsequent cycles, suggesting limited enzymatic adaptation. Co-exposure to phenol and iohexol disrupted nitrogen conversion, causing nitrite accumulation and increased total nitrogen, indicating strong nitrification inhibition. Long-term exposure altered the sludge microbiome, increasing biodiversity and metabolic richness as a sign of stress rather than improved function. Changes in carbon utilization suggested disturbed substrate transport and metabolic regulation. At the molecular level, transient monooxygenase induction, followed by reduced catechol-2,3-dioxygenase activity, indicated non-additive shifts in the pathway. Overall, phenol and iohexol negatively affected pollutant biotransformation, nitrogen cycling, and microbial metabolic functions. © 2026 Elsevier Ltd},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105035398286,
title = {Verification of the suitability of plant growth-promoting bacteria to support the removal of bisphenols from contaminated waters using Phragmites australis based floating treatment wetlands},
author = { M. Noszczyńska and M. Mitera and G. Zając and M. Rudnicka and M. Pacwa-Płociniczak and A. Nycz and M. Wojtkowska and M. Przybyla-Kasperek and R. Skinderowicz and T. Płociniczak},
url = {https://www.scopus.com/pages/publications/105035398286?origin=resultslist},
doi = {10.1016/j.ibiod.2026.106339},
year = {2026},
date = {2026-01-01},
journal = {International Biodeterioration and Biodegradation},
volume = {212},
publisher = {Elsevier Ltd},
abstract = {Bisphenols A (BPA), F (BPF), and S (BPS) are increasingly detected in water, posing a serious threat to living organisms. Although plant growth-promoting bacteria (PGPB) are considered a promising tool for bioremediation, it remains unknown whether immobilized root-associated PGPB can improve phytoremediation of bisphenol-contaminated water and help plants maintain physiological performance under pollutant stress. Thus, this study examined the potential of Phragmites australis and an immobilized bacterial consortium composed of two plant growth-promoting and bisphenol-utilizing bacterial strains to remove BPA, BPF, and BPS from contaminated water and assessed their effects on macrophyte performance under controlled microcosm conditions. The strains were immobilized in alginate beads and tested in bacteria-only, bacterial-assisted phytoremediation, and non-inoculated phytoremediation microcosms containing these three bisphenols. After 21 days, the planted microcosms achieved high removal efficiencies for all bisphenols (93.6–99.9%), whereas the bacteria-only treatment showed only slow BPF removal and no measurable removal of BPA or BPS. In addition, the inoculated bacteria survived poorly in the bacteria-only treatment but increased in abundance in the bacterial-assisted phytoremediation microcosm. Bacterial inoculation also did not reduce H2O2 or MDA levels in P. australis leaves, nor did it improve leaf biomass under bisphenol exposure. However, inoculated plants showed altered chlorophyll fluorescence parameters and higher expression of PGK, PRK, and RbcS genes, suggesting physiological support to the photosynthetic apparatus. Plant inoculation did not significantly enhance bisphenol removal efficiency, indicating that the intrinsic plant-associated microbial community was already highly effective in degrading these compounds under the tested conditions. © 2026 Elsevier Ltd},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105033113733,
title = {Auxin–cytokinin crosstalk and differential growth: complex relationships driving carpel initiation},
author = { D. Kwiatkowska and D. Borowska-Wykręt},
url = {https://www.scopus.com/pages/publications/105033113733?origin=resultslist},
doi = {10.1093/jxb/erag009},
year = {2026},
date = {2026-01-01},
journal = {Journal of Experimental Botany},
volume = {77},
number = {6},
pages = {1520-1524},
publisher = {Oxford University Press},
abstract = {[No abstract available]},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105031631538,
title = {The role of nitrilases in auxin biosynthesis, plant development and stress responses},
author = { B. Wójcikowska},
url = {https://www.scopus.com/pages/publications/105031631538?origin=resultslist},
doi = {10.1093/pcp/pcaf152},
year = {2026},
date = {2026-01-01},
journal = {Plant and Cell Physiology},
volume = {67},
number = {2},
pages = {115-129},
publisher = {Oxford University Press},
abstract = {NITRILASEs (NITs) are enzymes that have been identified across kingdoms. NITs are industrially important hydrolases, which are widely used in the production of valuable chemicals and medicines. In plants, NITs are phylogenetically divided into two groups: NIT1 and NIT4. The NIT1 (NIT1–3) subfamily that detoxifies nitriles is specific to the Brassicaceae and catalyzes the conversion of indole-3-acetonitrile, derived from indole glucosinolates or indole-3-acetaldoxime, into indole-3-acetic acid, the principal auxin, which provides an evolutionary advantage as it is a growth hormone. The NIT1 subfamily has been implicated in the catabolism of indole acetamide, although this has yet to be confirmed in planta. NIT4 appears to function in cyanide detoxification and exhibits strong specificity toward β-cyanoalanine. Additionally, it is hypothesized that NIT4, as well as enzymes of the NIT1 subfamily, might be involved in phenylacetic acid formation from phenylacetonitrile/benzyl cyanide. Crop plants, such as Zea mays and Oryza sativa, have been used to study NITs sporadically, consequently, our understanding of the role of NITs is primarily derived from studies of the model plant Arabidopsis thaliana, including single or sparse multiple mutants, reporter lines, or overexpressing lines. This review mainly focuses on the NIT1 subfamily, which plays a role in root and flower development. However, NITs expression and activity have been demonstrated mainly under plant stress conditions, including both biotic and abiotic stresses, such as saline, drought, sulfate deficiency, and thermomorphogenesis, during which NIT-dependent auxin biosynthesis is activated. In addition, the role of NITs has been confirmed in morphogenetic processes in in vitro cultures, highlighting their role in stress-induced developmental reprogramming. © The Author(s) 2025. Published by Oxford University Press on behalf of the Japanese Society of Plant Physiologists.},
note = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105036453792,
title = {Advancing soil health with biochar - Effects on soil microbial activity and diversity},
author = { A. El-Aradi and A. Nowak and T. Kasim and D.J. Webb and D.J. Nowakowski},
url = {https://www.scopus.com/pages/publications/105036453792?origin=resultslist},
doi = {10.1016/j.scitotenv.2026.181808},
year = {2026},
date = {2026-01-01},
journal = {Science of the Total Environment},
volume = {1031},
publisher = {Elsevier B.V.},
abstract = {Improving soil quality is essential for sustainable agriculture and biochar is a promising soil amendment because its effects on soil chemistry and microbial functioning depend strongly on feedstock and production conditions. This study compared wheat straw biochar (WSBC) and pine wood biochar (PWBC) by characterising their physicochemical and safety properties and evaluating their effects on agricultural soil during a six-month incubation at 1, 2.5 and 5% (v/v) amendment rates. Soil physicochemical properties, total heterotrophic bacteria, dehydrogenase activity and community-level physiological profiles were determined. Both biochar samples met international safety standards for the parameters tested. WSBC had higher ash and nutrient contents, higher pH and higher electrical conductivity than PWBC and exerted the stronger short-term effect on soil properties. WSBC increased soil pH from 6.6 to 7.1 at the 2.5% application rate, raised electrical conductivity from 300 to 570 μS/cm at 5% and increased plant-available K from 289 to 849 mg/L. PWBC caused only minor changes in soil pH, electrical conductivity and K. Total Kjeldahl N, extractable P, total organic carbon and organic matter remained relatively stable across treatments. Total heterotrophic bacteria declined by about three log units by day 30 in all soils, indicating no specific biochar effect on bacterial abundance. Dehydrogenase activity peaked on day 90, increasing 4.3-fold at 5% WSBC and 1.95-fold at 5% PWBC relative to the control. By day 180, microbial metabolic profiles showed increased utilisation of more chemically complex substrates, particularly polymers and surfactants. These results indicate that WSBC acts primarily as a liming and nutrient-supplying amendment that stimulates microbial activity, whereas PWBC behaves as a more inert, carbon-rich material with limited short-term effects on soil chemistry. Biochar addition did not disrupt soil biological functioning, but promoted time-dependent shifts in microbial metabolism. © 2026 The Authors},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105037042048,
title = {Berberine Toxicity Profile in Experimental Models as a Basis for Assessing Its Biological Safety},
author = { A. Karczmarzyk and D. Wojcieszyńska and A. Nowak and W. Smułek and U. Guzik},
url = {https://www.scopus.com/pages/publications/105037042048?origin=resultslist},
doi = {10.3390/molecules31081350},
year = {2026},
date = {2026-01-01},
journal = {Molecules},
volume = {31},
number = {8},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {Berberine, a natural alkaloid, is a substance widely used in natural medicine. However, there is a significant knowledge gap regarding the potential negative effects of higher environmental concentrations of berberine resulting from its use as a supplement. Therefore, the aim of this study was to assess its toxicity towards microorganisms and organisms from various trophic levels. The results indicate that berberine may influence the reorganization of bacterial membranes, thereby negatively impacting the environmental microbiome. However, oxidative cell damage, a phenomenon commonly described in the literature, was not demonstrated. At the concentrations used, berberine may even have a protective effect. The analysis of toxicity towards Tetrahymena, Selenastrum, and Heterocypris indicated a similar level of berberine toxicity across these organisms, suggesting that the toxic effect is not species-dependent and that the mechanism of toxicity is probably based on universal cellular mechanisms. © 2026 by the authors.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105038287612,
title = {Microbial Transformation of Agriculture-Related Microplastics in Aquatic Environments},
author = { S. Piekarz and T. Płociniczak and M. Noszczyńska},
url = {https://www.scopus.com/pages/publications/105038287612?origin=resultslist},
doi = {10.3390/agriculture16090921},
year = {2026},
date = {2026-01-01},
journal = {Agriculture (Switzerland)},
volume = {16},
number = {9},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {Microplastics (MPs), defined as plastic particles ranging in size from 0.1 μm to 5 mm, have gained significant scientific attention worldwide due to their widespread occurrence and potential risks for human health and the environment. MPs can accumulate in water and soil, affecting organisms across multiple trophic levels and negatively impacting agricultural productivity and animal husbandry. Agricultural practices, such as plastic mulching, compost, and sewage sludge application, contribute to environmental plastic contamination, while irrigation and wastewater reuse facilitate their transport and deposition across ecosystems. Given the limited efficiency and high costs of physicochemical remediation methods, microbial biodegradation has attracted growing attention as a potentially sustainable strategy. This review focuses primarily on the metabolic potential of bacteria and fungi and the mechanisms underlying MP degradation. In the context of environmental safety, such studies are of particular importance. Under optimal laboratory conditions, reported microbial degradation efficiencies varied with microplastic type, microbial strain(s), and experimental conditions, ranging from 4% to >97%. Moreover, the literature review identifies key barriers to practical application, including environmental variability and the limited transferability of laboratory findings to field settings. Future research should therefore prioritize testable, application-oriented approaches. Addressing these gaps is essential to developing effective microbial degradation strategies for mitigating microplastic pollution. © 2026 by the authors.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
@article{2-s2.0-105039514061,
title = {The transcription factor LEC2 as an epigenetic regulator of plant totipotency: from Arabidopsis to crop improvement},
author = { M.D. Gaj},
url = {https://www.scopus.com/pages/publications/105039514061?origin=resultslist},
doi = {10.1007/s13353-026-01065-8},
year = {2026},
date = {2026-01-01},
journal = {Journal of Applied Genetics},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Plant cell totipotency—the ability of a differentiated somatic cell to regenerate a complete organism—is one of the most remarkable features of plant development and a cornerstone of biotechnology. Somatic embryogenesis (SE) represents the most striking manifestation of this potential, serving as a powerful system for clonal propagation, genetic transformation, and functional genomics. At the molecular level, SE is governed by transcription factors (TFs) that integrate hormonal and metabolic signals with chromatin remodeling to activate embryogenic programs in somatic cells. Among SE-related TFs, LEAFY COTYLEDON2 (LEC2) of Arabidopsis thaliana has emerged as an essential regulator of embryogenic transition. LEC2 functions both as an auxin-responsive developmental switch and as an epigenetic modulator that recruits chromatin regulators to reprogram transcriptional networks controlling the totipotency of plant cells. This review (i) outlines the utility of Arabidopsis as a model for studying SE and totipotency, (ii) highlights the dual role of LEC2 as both a target and an architect of epigenetic regulation, and (iii) explores translational perspectives for TF-based strategies in plant regeneration and crop improvement. The evidence demonstrating that LEC2 coordinates transcriptional regulation with epigenetic remodeling, acting as a central hub of plant totipotency, is summarized and illustrated graphically. LEC2-centered insights into SE not only deepen our understanding of the molecular basis of plant totipotency but also provide a conceptual framework for improving regeneration efficiency across plant species. © The Author(s) 2026.},
note = {0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In: ACS Biomaterials Science and Engineering, vol. 12, no. 5, pp. 2619-2635, 2026, (0). Effects of resveratrol and nanodiamonds on sirtuin activity, oxidative stress and DNA damage in Acheta domesticus Journal Article In: Biochemical and Biophysical Research Communications, vol. 816, 2026, (1). Bisphenol A disrupts epithelial homeostasis in the midgut of the freshwater shrimp Neocaridina davidi Journal Article In: European Zoological Journal, vol. 93, no. 1, pp. 563-582, 2026, (0). In: Materials, vol. 19, no. 8, 2026, (0). Temperature-dependent bistable electrical switching in hydrated barley genomic DNA Journal Article In: BioSystems, vol. 263, 2026, (0). Evaluation of Molecular Responses and Longevity Markers in Acheta domesticus Following Combined Resveratrol and Nanodiamond Exposure Journal Article In: International Journal of Molecular Sciences, vol. 27, no. 6, 2026, (0). Evaluation of non-opioid analgesic toxicity and their effects on native and invasive species of crustaceans (Gammaridae, Gammarus) Journal Article In: BioRisk, no. 24, pp. 85-102, 2026, (0). Nuclear DNA Content Analyses by Flow Cytometry of Saffron (Crocus sativus L.) Populations Obtained from Safranbolu, Türkiye Journal Article In: Current Issues in Molecular Biology, vol. 48, no. 3, 2026, (0). Cr(VI) detoxification from water using a regenerable fullerene-based hybrid adsorbent with in vivo safety assessment Journal Article In: Water Resources and Industry, vol. 35, 2026, (0). Response to multigenerational graphene oxide exposure in acheta domesticus strains selected for longevity Journal Article In: Scientific Reports, vol. 16, no. 1, 2026, (0). Yersinia enterocolitica O:3 Outer Membrane Vesicles as a Platform for Complement Activation Journal Article In: Journal of Extracellular Vesicles, vol. 15, no. 4, 2026, (0). In: Insects, vol. 17, no. 2, 2026, (0). A synergistic-like interaction of phenol and iohexol on the structure and functioning of the activated sludge microbiome Journal Article In: International Biodeterioration and Biodegradation, vol. 212, 2026, (0). In: International Biodeterioration and Biodegradation, vol. 212, 2026, (0). Auxin–cytokinin crosstalk and differential growth: complex relationships driving carpel initiation Journal Article In: Journal of Experimental Botany, vol. 77, no. 6, pp. 1520-1524, 2026, (0). The role of nitrilases in auxin biosynthesis, plant development and stress responses Journal Article In: Plant and Cell Physiology, vol. 67, no. 2, pp. 115-129, 2026, (1). Advancing soil health with biochar - Effects on soil microbial activity and diversity Journal Article In: Science of the Total Environment, vol. 1031, 2026, (0). Berberine Toxicity Profile in Experimental Models as a Basis for Assessing Its Biological Safety Journal Article In: Molecules, vol. 31, no. 8, 2026, (0). Microbial Transformation of Agriculture-Related Microplastics in Aquatic Environments Journal Article In: Agriculture (Switzerland), vol. 16, no. 9, 2026, (0). The transcription factor LEC2 as an epigenetic regulator of plant totipotency: from Arabidopsis to crop improvement Journal Article In: Journal of Applied Genetics, 2026, (0).